LOCUS       DACDBG010000001        86050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDBG010000001 DACDBG010000000
VERSION     DACDBG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.001"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..519)
                     /locus_tag="IVZ90_000001"
     CDS             complement(79..519)
                     /locus_tag="IVZ90_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8261083.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(648..1574)
                     /gene="dnaI"
                     /locus_tag="IVZ90_000002"
     CDS             complement(648..1574)
                     /gene="dnaI"
                     /locus_tag="IVZ90_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8261084.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(1574..2947)
                     /locus_tag="IVZ90_000003"
     CDS             complement(1574..2947)
                     /locus_tag="IVZ90_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8261085.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(2970..3461)
                     /gene="nrdR"
                     /locus_tag="IVZ90_000004"
     CDS             complement(2970..3461)
                     /gene="nrdR"
                     /locus_tag="IVZ90_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8261086.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(3773..4105)
                     /locus_tag="IVZ90_000005"
     CDS             complement(3773..4105)
                     /locus_tag="IVZ90_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8261087.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(4157..4786)
                     /locus_tag="IVZ90_000006"
     CDS             complement(4157..4786)
                     /locus_tag="IVZ90_000006"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8261088.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(4750..5586)
                     /gene="mutM"
                     /locus_tag="IVZ90_000007"
     CDS             complement(4750..5586)
                     /gene="mutM"
                     /locus_tag="IVZ90_000007"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8261089.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(5631..8276)
                     /gene="polA"
                     /locus_tag="IVZ90_000008"
     CDS             complement(5631..8276)
                     /gene="polA"
                     /locus_tag="IVZ90_000008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8261090.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(8402..9085)
                     /locus_tag="IVZ90_000009"
     CDS             complement(8402..9085)
                     /locus_tag="IVZ90_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8261091.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(9078..9563)
                     /locus_tag="IVZ90_000010"
     CDS             complement(9078..9563)
                     /locus_tag="IVZ90_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8261092.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(9991..11325)
                     /locus_tag="IVZ90_000011"
     CDS             complement(9991..11325)
                     /locus_tag="IVZ90_000011"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8261093.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(11456..12217)
                     /locus_tag="IVZ90_000012"
     CDS             complement(11456..12217)
                     /locus_tag="IVZ90_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8261094.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(12233..12715)
                     /locus_tag="IVZ90_000013"
     CDS             complement(12233..12715)
                     /locus_tag="IVZ90_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8261095.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(12735..14915)
                     /locus_tag="IVZ90_000014"
     CDS             complement(12735..14915)
                     /locus_tag="IVZ90_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8261096.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(15059..15790)
                     /locus_tag="IVZ90_000015"
     CDS             complement(15059..15790)
                     /locus_tag="IVZ90_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8261097.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(15859..16800)
                     /locus_tag="IVZ90_000016"
     CDS             complement(15859..16800)
                     /locus_tag="IVZ90_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8261098.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(16980..17347)
                     /locus_tag="IVZ90_000017"
                     /pseudo
     CDS             complement(16980..17347)
                     /locus_tag="IVZ90_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(17958..19349)
                     /locus_tag="IVZ90_000018"
     CDS             complement(17958..19349)
                     /locus_tag="IVZ90_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8261099.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(19562..19948)
                     /locus_tag="IVZ90_000019"
     CDS             complement(19562..19948)
                     /locus_tag="IVZ90_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8261100.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(20004..20627)
                     /locus_tag="IVZ90_000020"
     CDS             complement(20004..20627)
                     /locus_tag="IVZ90_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8261101.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(20691..21359)
                     /locus_tag="IVZ90_000021"
     CDS             complement(20691..21359)
                     /locus_tag="IVZ90_000021"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8261102.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(21371..21811)
                     /locus_tag="IVZ90_000022"
     CDS             complement(21371..21811)
                     /locus_tag="IVZ90_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261103.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(21870..22542)
                     /locus_tag="IVZ90_000023"
                     /pseudo
     CDS             complement(21870..22542)
                     /locus_tag="IVZ90_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(22727..23125)
                     /locus_tag="IVZ90_000024"
     CDS             complement(22727..23125)
                     /locus_tag="IVZ90_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8261104.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(23122..23895)
                     /locus_tag="IVZ90_000025"
     CDS             complement(23122..23895)
                     /locus_tag="IVZ90_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8261105.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(23892..24575)
                     /locus_tag="IVZ90_000026"
     CDS             complement(23892..24575)
                     /locus_tag="IVZ90_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8261106.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(24742..25629)
                     /locus_tag="IVZ90_000027"
     CDS             complement(24742..25629)
                     /locus_tag="IVZ90_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8261107.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(25626..26495)
                     /locus_tag="IVZ90_000028"
     CDS             complement(25626..26495)
                     /locus_tag="IVZ90_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8261108.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(26632..27918)
                     /locus_tag="IVZ90_000029"
     CDS             complement(26632..27918)
                     /locus_tag="IVZ90_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8261109.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            28191..28769
                     /locus_tag="IVZ90_000030"
     CDS             28191..28769
                     /locus_tag="IVZ90_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8261110.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            28769..29698
                     /locus_tag="IVZ90_000031"
     CDS             28769..29698
                     /locus_tag="IVZ90_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8261111.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29695..31431
                     /locus_tag="IVZ90_000032"
     CDS             29695..31431
                     /locus_tag="IVZ90_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8261112.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(31494..32330)
                     /gene="pheA"
                     /locus_tag="IVZ90_000033"
     CDS             complement(31494..32330)
                     /gene="pheA"
                     /locus_tag="IVZ90_000033"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8261113.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(32327..32836)
                     /locus_tag="IVZ90_000034"
     CDS             complement(32327..32836)
                     /locus_tag="IVZ90_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8261114.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(32829..34124)
                     /gene="aroA"
                     /locus_tag="IVZ90_000035"
     CDS             complement(32829..34124)
                     /gene="aroA"
                     /locus_tag="IVZ90_000035"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8261115.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(34137..35228)
                     /locus_tag="IVZ90_000036"
     CDS             complement(34137..35228)
                     /locus_tag="IVZ90_000036"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8261116.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(35245..36411)
                     /gene="aroC"
                     /locus_tag="IVZ90_000037"
     CDS             complement(35245..36411)
                     /gene="aroC"
                     /locus_tag="IVZ90_000037"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8261117.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(36413..37477)
                     /gene="aroB"
                     /locus_tag="IVZ90_000038"
     CDS             complement(36413..37477)
                     /gene="aroB"
                     /locus_tag="IVZ90_000038"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8261118.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(37510..38532)
                     /gene="aroF"
                     /locus_tag="IVZ90_000039"
     CDS             complement(37510..38532)
                     /gene="aroF"
                     /locus_tag="IVZ90_000039"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8261119.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(39191..40165)
                     /locus_tag="IVZ90_000040"
     CDS             complement(39191..40165)
                     /locus_tag="IVZ90_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8261120.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(40146..42029)
                     /locus_tag="IVZ90_000041"
     CDS             complement(40146..42029)
                     /locus_tag="IVZ90_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8261121.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(42047..44272)
                     /locus_tag="IVZ90_000042"
     CDS             complement(42047..44272)
                     /locus_tag="IVZ90_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8261122.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            44387..45421
                     /locus_tag="IVZ90_000043"
     CDS             44387..45421
                     /locus_tag="IVZ90_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8261123.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(45488..46501)
                     /locus_tag="IVZ90_000044"
     CDS             complement(45488..46501)
                     /locus_tag="IVZ90_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8261124.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(46579..46767)
                     /locus_tag="IVZ90_000045"
     CDS             complement(46579..46767)
                     /locus_tag="IVZ90_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8261125.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(47207..47335)
                     /locus_tag="IVZ90_000046"
     CDS             complement(47207..47335)
                     /locus_tag="IVZ90_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8261126.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(47338..48711)
                     /gene="rlmD"
                     /locus_tag="IVZ90_000047"
     CDS             complement(47338..48711)
                     /gene="rlmD"
                     /locus_tag="IVZ90_000047"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8261127.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(48822..49877)
                     /locus_tag="IVZ90_000048"
     CDS             complement(48822..49877)
                     /locus_tag="IVZ90_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8261128.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(49887..51317)
                     /gene="gatB"
                     /locus_tag="IVZ90_000049"
     CDS             complement(49887..51317)
                     /gene="gatB"
                     /locus_tag="IVZ90_000049"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8261129.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(51314..52786)
                     /gene="gatA"
                     /locus_tag="IVZ90_000050"
     CDS             complement(51314..52786)
                     /gene="gatA"
                     /locus_tag="IVZ90_000050"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8261130.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(52786..53091)
                     /gene="gatC"
                     /locus_tag="IVZ90_000051"
     CDS             complement(52786..53091)
                     /gene="gatC"
                     /locus_tag="IVZ90_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8261131.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(53197..55233)
                     /gene="ligA"
                     /locus_tag="IVZ90_000052"
     CDS             complement(53197..55233)
                     /gene="ligA"
                     /locus_tag="IVZ90_000052"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8261132.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(55250..57484)
                     /gene="pcrA"
                     /locus_tag="IVZ90_000053"
     CDS             complement(55250..57484)
                     /gene="pcrA"
                     /locus_tag="IVZ90_000053"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8261133.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            57639..59198
                     /locus_tag="IVZ90_000054"
     CDS             57639..59198
                     /locus_tag="IVZ90_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8261134.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(59309..61240)
                     /locus_tag="IVZ90_000055"
     CDS             complement(59309..61240)
                     /locus_tag="IVZ90_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8261135.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(61258..62172)
                     /gene="pfkB"
                     /locus_tag="IVZ90_000056"
     CDS             complement(61258..62172)
                     /gene="pfkB"
                     /locus_tag="IVZ90_000056"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8261136.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(62169..62921)
                     /locus_tag="IVZ90_000057"
     CDS             complement(62169..62921)
                     /locus_tag="IVZ90_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8261137.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(63080..63289)
                     /locus_tag="IVZ90_000058"
     CDS             complement(63080..63289)
                     /locus_tag="IVZ90_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261138.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(63300..64628)
                     /locus_tag="IVZ90_000059"
     CDS             complement(63300..64628)
                     /locus_tag="IVZ90_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261139.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(64993..65580)
                     /locus_tag="IVZ90_000060"
     CDS             complement(64993..65580)
                     /locus_tag="IVZ90_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8261140.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(65663..66913)
                     /gene="clpX"
                     /locus_tag="IVZ90_000061"
     CDS             complement(65663..66913)
                     /gene="clpX"
                     /locus_tag="IVZ90_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8261141.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(67160..68449)
                     /locus_tag="IVZ90_000062"
     CDS             complement(67160..68449)
                     /locus_tag="IVZ90_000062"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8261142.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(68603..69535)
                     /locus_tag="IVZ90_000063"
     CDS             complement(68603..69535)
                     /locus_tag="IVZ90_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261143.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(69692..70351)
                     /locus_tag="IVZ90_000064"
     CDS             complement(69692..70351)
                     /locus_tag="IVZ90_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8261144.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(70354..71379)
                     /gene="sppA"
                     /locus_tag="IVZ90_000065"
     CDS             complement(70354..71379)
                     /gene="sppA"
                     /locus_tag="IVZ90_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8261145.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(71392..71682)
                     /locus_tag="IVZ90_000066"
     CDS             complement(71392..71682)
                     /locus_tag="IVZ90_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261146.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            72091..73896
                     /gene="glmS"
                     /locus_tag="IVZ90_000067"
     CDS             72091..73896
                     /gene="glmS"
                     /locus_tag="IVZ90_000067"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8261147.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(73968..75323)
                     /locus_tag="IVZ90_000068"
     CDS             complement(73968..75323)
                     /locus_tag="IVZ90_000068"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8261148.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(75347..76516)
                     /locus_tag="IVZ90_000069"
     CDS             complement(75347..76516)
                     /locus_tag="IVZ90_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261149.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(76513..77400)
                     /locus_tag="IVZ90_000070"
     CDS             complement(76513..77400)
                     /locus_tag="IVZ90_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8261150.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(77589..81281)
                     /gene="nifJ"
                     /locus_tag="IVZ90_000071"
     CDS             complement(77589..81281)
                     /gene="nifJ"
                     /locus_tag="IVZ90_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8261151.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            81480..82001
                     /locus_tag="IVZ90_000072"
     CDS             81480..82001
                     /locus_tag="IVZ90_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8261152.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(82700..83257)
                     /locus_tag="IVZ90_000073"
     CDS             complement(82700..83257)
                     /locus_tag="IVZ90_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261153.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(83436..84047)
                     /locus_tag="IVZ90_000074"
     CDS             complement(83436..84047)
                     /locus_tag="IVZ90_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261154.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(84160..84417)
                     /locus_tag="IVZ90_000075"
     CDS             complement(84160..84417)
                     /locus_tag="IVZ90_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261155.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(85014..85292)
                     /locus_tag="IVZ90_000076"
     CDS             complement(85014..85292)
                     /locus_tag="IVZ90_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261156.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(85326..85577)
                     /locus_tag="IVZ90_000077"
     CDS             complement(85326..85577)
                     /locus_tag="IVZ90_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261157.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        23800 a        18659 c        15113 g        28478 t
ORIGIN      
        1 attcccatat ctccttaaaa aatatacctt ttcgttctat aaaacaacat tttcttattt
       61 ttgaaaaaat gttattggtt atttgttcaa tgaaacatca ataatcgaat ctccaggaac
      121 ttgcggtggg atagacatcg tcatgatcgg catctcaact aatgtaaatt ggactttgtc
      181 accgattttc aattcattta tgtccacgct tattttttca gcagtagcat ttaagatcac
      241 tccgtcattg ctgaatgacg ttcccatatt cgctggatct tctttggcca tgacatcgac
      301 gagtttcaac tgaagcgttt cttcttctgc ttctacttga gtaatacttt caatcgttcc
      361 gacaaatatg ctttgtctct ccgttttcgt actgcttgtt tttactgttt gacccgcatc
      421 aatcgaatca acagaacctg tgttgctatc tgcgctaccg cagcctgcta aaagaaaaaa
      481 tagaccaact acagatatac tagaaaaaat agccctcata aaaaatcctc cgtttaagtt
      541 tctgcattac ttgttgcggt gattgtctcc ggtcatttct gtccatataa tgattcgctg
      601 acctgctttt actactaggt caaatgtcag ttggacttgt caactgttca accattgcgt
      661 cggttgcgac cagtcatttc gatttctttt gtcaaataac gtatgcgttc cattatccgt
      721 ttcgctttta gcggctcttc atctccacgt tgagtgacgg acaaatgctg ttccaattca
      781 tccattgtaa aattcgatga gaagaaggtt ggtaactgtt cctgcatccg atattgaagg
      841 ataacaccga atacttcgtc tcgtatccaa ctactcatcg catctgcacc aatgtcatcg
      901 atcatcaaaa taggtgaacg tttcactgca tctaactttt ctgccacttg atcttttccg
      961 attgcctgtt tcatctcaac ggcaaatgtc ggaaaatgga ctagcgtcgt ggtgaatcca
     1021 gcaatcgcta attctctcgc tgttgctcct aataaatatg tttttccaat accaaaactt
     1081 cctactagat acagtccttt atgataccct tttggatcag cagtatattt ctctacaaaa
     1141 tctagggctt ctaagctcgc atatcctcgt ccatctgtac gttcaaagtt ttcaaaagaa
     1201 gccgattgaa tatcttttgg catatccatt gcttttactc ggcttctgat ttcttcttgt
     1261 ctttgccgtg ctaacaatgc ttccgttggt acataagtga catccacata atgaaaattc
     1321 aacgtcagct tcggctcata gccgggagcg atcatcgttg gatcgttttt ttgatattta
     1381 cgcttttctt gcacgaattc atacaattta gcgtaacttt tttcaatatc cgcttgtgtc
     1441 aattcttctt ggtgtgcgct tagaaaagct tgaacatccg catcatttaa gacttcagcg
     1501 atcatttcag catacttttc ttgataattg cgtttgctga tgatcctctt caattctttg
     1561 cctacgtctt ccactagata tcaccctcct tttttgccag gtattcttta aaacggcggt
     1621 caatctctgc ttttttctcg ctgctgatct gtttttcatc ttttggctgg ttgacccaat
     1681 caggaagctt ttcccgtcga acatttcgct tgccagtttg ataatttgtt tgcttctttt
     1741 gttttgattg ttttgcttga ttacttattt gccgaatgtg ctggatcgct tcttctgctg
     1801 tggcgatttt cttttgtgcc cattcatttg ccactgtatt tacataactg gcatttaagc
     1861 tagcattatt cttgatcact agtacataat tcagcaaaac attgatcacg ctactggaaa
     1921 gacctgattg agaaacaaga tcttgcagta accagttttc ttgtttagaa acatatccgc
     1981 ctttttctga cttgattgct tccagatatt tcatcggtgg aatgctctgt gcttctttta
     2041 tcagctgaag cgctgcgttt gtcaaatgtt cgtttgacga gggttcatta ctgttttcag
     2101 aaaccgcaac tgtttctttt gtttgcttca ccggacgtct ctgcattgcc aaaatggctt
     2161 tttgcagttc tttttcagat actttttgtt cactaaagtc ataagattga ctgatcagtt
     2221 cgccaagtgt caattcatcg aaaccgtaaa gctgatgata catgtcgact tgtttgatga
     2281 ttggatcatc aagacttgca atatgttttt ttgccagcca atcattcaaa aactgccaat
     2341 cgaatgtcga atctgcttca agtaatgcta ctgattcaga ttcttcaaaa gatttattca
     2401 gcgattcgat gttttcttga ttagcagcgt acactgcttc attgaagcga taaacatcta
     2461 aaaatttttt tgtgattttt tgatatccct ctgctgtatg ccttttaggt tcaaagaatt
     2521 gggtaagctt ctggaatcgt cgttcaccaa ctttttccag aagaagaaaa gccagaacag
     2581 agtctttgaa aaatgcttta ggaatgagtg gttctaacag ctcataaata aaacgaggtc
     2641 ccagatcact atcttcttta aaataaacat ccagtaagcc gattccttct agcttttttc
     2701 ttgcatcata aaattgcggg ataccgcagc ctagactact aagcagatct gcatgcatcc
     2761 cttcaggacc tttgccagat tcttgagaga tttctgataa cagggtgaaa tagatactca
     2821 aggcttctcc gccgattagt ggctgataaa gatacagcaa ggcgttcttg ctgtcttctc
     2881 tcagcggagc cgcttttctt ccttggtaaa tattttttgg ttgaagctct ttccaagcat
     2941 tttccaagga aatgcctcct ttttgtttct taattgtttt ctttattatt ctctttatta
     3001 ttttcttttg ctttatccac gatatcttgc aactctttta agaaaacaga catatctttg
     3061 aactggcgat aaacacttgc aaaacgaata taagcgattt cgtctaaatc aactaaatct
     3121 tccatcacat attcaccaat tagattcgtc gatacttcgt tttctccgag actgcgtacg
     3181 cggttttcga catggttgac gatttgttcc atttgttcca tagctacagg tcttttttct
     3241 gctgaacgga tgagtcctct taaaatttta tcgcgattga actcttcgcg ttccccgttt
     3301 ttcttgatta ctaataacgg agcagcttca attcgttcaa atgtagtgaa tcggaacgaa
     3361 caattctcac attcgcgacg gcgacggatc gcacgtccat catctgcttg acggctgtcg
     3421 attactcgtg aattattatg ttgacatctg ggacatctca ttttgtctca cctcttgcat
     3481 cgcattttat ataaaggatg tgtctttctt tacaactagt tgtattataa cacgaaacaa
     3541 atcacaaacg cgactgtcct acccaaaaaa cacggatata agaacaattt tttctacttt
     3601 catgcgtaat tgtgtcccct gtccttacta aatttcaatt ttccaatact gatcaccttg
     3661 attactgaga aataacttac acaaaagctg atttagtcat ctagcacaaa aaacttgaag
     3721 cagatgaaca ccttctcatc tgcttcaagt cacatctcga ttttttacta ttttataact
     3781 tctcttcgat tcctgtatag tgtttcattc gctgttcctc gattttttgc tcatcggaaa
     3841 tgtggtcact ttctgggagc ggtgttttac tacgatcagg tgtcggattt tttcgttctt
     3901 gagtcaaatc ttgccgcttc gcttcttctt ctgcctgctg gtcttctatt tcatcgattc
     3961 ttgcttggta ctgcttttct ttttcttctg ctgtttctga ttgcgtacga ccttcatcga
     4021 ttagattttc tttattgagc atctcgccga ttttttcttt tgcgttacct acgactttat
     4081 ccttttgatt ttttaatgat cccatcgcta tccctcactt tctatatact caggatagca
     4141 aaattgacac agatactcaa agaaagcgat tagcaaaaag ccaattttcg atttgtccca
     4201 caagtttttc ttttgttcca ttgttgtcga acaaaatatc cgcacgctgc tttttttctt
     4261 cgattggaag ttgactacta attctctgct gcgcttgttc tagcgtcagt tgattacgtt
     4321 tcatcaaacg ctgaagctga gtttctggcg tagtataaac gacagctact gcatccatat
     4381 agtggtcata atgaccttcg tagagcaatg ggatatctac aattactaaa ggatgattgg
     4441 aacttagaga ggcgatttga tcttttatcg cttttcggat aaacggatct aatagctgat
     4501 caagtttttg ccgtttttcc tttgatggaa aaacaatcat cccaagtttc cctcgatcta
     4561 gttgcccatc aggctgcaat acctcttggc caaacactga tacaattttc ctcagagcaa
     4621 gcgttccagg ctcaacagtt tctctggcaa tcacatcgcc atcgatcaaa ggaaatccat
     4681 gagactgaaa gactttggct gccgtacttt taccggtagc tattccaccc gtcaaaccta
     4741 aaacaaatcc tactttgcga tttttagcct ttgacaattc ggacaatagt gcgtccccct
     4801 ttgtgccact tttgttttca cgatcggcgt tccacaacgt atacatggtt tgccagtttg
     4861 ctgatagaca tgcagtgcta cttgaaatcc gcctgattct cctaatgcat tcaaataact
     4921 gcgaatcgtg gtacctccgg cttctattgc acgatccaaa acatcaatga tcgatagacg
     4981 taattgttct acctctttgg ggcgcagtgt gttcgccggt tgttctggat ggatcttcgc
     5041 ttcccataat gcttcatcta cataaatatt acctagacca gtcactagac gttggtctag
     5101 cagcaatggc ttgattgctt tgtgggactt tttcagtcca tttgcgaaat ctgctaataa
     5161 gaagctatct ggcaaaggct caggtcccaa tttcatgatt cctcggtaag ttgcacttgc
     5221 tcctttttcg acgattgtca tacgaccaaa ttttcgtaca tcgttgtagc gaagttggct
     5281 tccatcctca aaaaagaaaa gtacatgcgt atgtttatcg attggtgtat tttctttcgt
     5341 aaattgatat tttccttcca ttcgcaagtg tgagatcagt tcacaatgat cgagatgaaa
     5401 aattaaaaac ttccctctac gtccaatgga ttggatggtt tcgccaacaa gagaagcctc
     5461 aaagatgggt gtctctggtt gctcgatgat cttgggccac aagacctgaa ctttttgaat
     5521 cgtttttcct actactaacc gttccaatcc tttacgaacg gtttctactt ctggtaattc
     5581 tggcattttt tcttttcacc ttcattatat tgctctgtct taaacaatgt ttattttgct
     5641 tcgtaccacg tttttcccca actgctgtca gtgatcagtg gtacatgaag agaaacagct
     5701 tgttccataa cttccttcac caatttatct aatgattcaa gttctttttc cggtacctcg
     5761 aagacaagtt catcatgaac ttgtaacagc atcgtagctt gaagacctgt ttcttttaat
     5821 cgtttatcca attcgatcat cgcgattttc aaaatatcag cagcacttcc ttggattggt
     5881 gtattgatag ctgttcgttc cgcaaaagaa cggatattgt aatttcttga attgatgtct
     5941 ggcagatatc gacggcggtg atacagcgtt tcgacgtagc cttggtcttt tgcttcgcgt
     6001 acgattcttt ccatatattc tttgacacct gggtattttt caaagtacgt atcgatgtat
     6061 tgctgtgctg cttttcggct gattcctaga ttttgtgaaa gtccgtaatc agagatgcca
     6121 tacacgatac caaaattcac tgcttttgcc tgcctacgca tatttggtgt gacatcctct
     6181 gccttttcga ttccaaatac acgcatcgct gtgctggcat ggatatcttg accttctagg
     6241 aaggcagctt tcaaatgttc atcatttgag atatgcgcaa gtacgcgtaa ctcgatttgt
     6301 gaatagtcag aagaataaat cagccagtct ttttctctag gaacaaatgc ctgacgtatc
     6361 tttctccctt catctaatcg aatcgggatg ttctgcaaat taggatcaac agagcttaat
     6421 cgtcctgttt gggtcaatgt ctggacataa cgggtatgca cttttccatc tgattggatc
     6481 actttcagca gtccctcgac ataggtagat tgaatttttg caatttgacg ataggtgagg
     6541 atatcctcta cgattggtgc ttgttcacga agctgttcca gtacatctac ggctgtggaa
     6601 tatccagttt ttgttttttt gataacgggt aatcccattt tttcaaataa gatcactcct
     6661 aattgctttg gtgaattcaa attgaattct tcgccggctt cttcgtagat tttttgttcg
     6721 atctctctta agcgagcaga gaactcgccc ttcatttctt gtaagcgagt cgcatctact
     6781 ttgatcccgc ttatttccat ctctgccaaa atgatagaca atggcagttc cattttccgg
     6841 aaaagatctt cttggttttt atctgccagt tcttggctca attttggtgt caacgcattg
     6901 attgcagcta cttttcgcgc taaatgagca aaaaagagtt cctcttcttc tggtaaacct
     6961 tttttcgccc ctttgccgta tacagcttca tctgattgga tatcgttgta tccgtaatga
     7021 gcagctattc cttcgatatc tgtgcttttt tcattcgtat cgagaaggta tgcacctagc
     7081 aagacatcaa aatcgattcc tttcgttttt ccagtatagc ggtttaaagc aacataagtt
     7141 cgtttcgcat catatacttt tttcagccga gtcggatcag tgatccaaga atggaacgct
     7201 cggttttcga atacagatag atcgttcgtg acataaattt tcttttctgt cccccacgct
     7261 actccgacga tttcctctgt atgataattg tcgcccatca tttccacgta caacgccata
     7321 tccgtagtaa acatttcctc tttgaattca tgaacgactt caaacaaaat atcatccatt
     7381 tttacttctt cttctacgat attcaattta gaaaggaact gattgaaatc catttcttta
     7441 taaaacggaa cgagtttatc gagatttttc ccttcatact tcaatgagtc gatagagaca
     7501 tccacaggcg ctgaagtatt gatggtcgcc aattttttcg ataagaaagc ctgctctttg
     7561 tcatttatca gattttcttt cattttgctt tgcttcattt catcgatatg ttcataaatt
     7621 ccttcgactg agccatattg tttcaatagc ttgatagctg ttttttcacc gattttagtc
     7681 acaccgggga tattgtcaga ggcatctccg gctagtccct tcatgtcaat aatctgtttt
     7741 ggcgtcaacc catcatattt ttccgctaca tgttctggtg tatagctttc gatatcgctg
     7801 acacctttta cagtgatatc cacttttact tctttactag ctaattgagt caaatcccga
     7861 tcacctgaaa gcacgacgac atcaaattga tctttgtcta ctttttctgc caaagtaccg
     7921 ataatatcat ctgcttcata attgggcaat tcgtaatact gtacccctag tcctgtcaac
     7981 aaatcacgta tatatggcat ttgttctttg aattcgccag gtgttttgga tcttccacct
     8041 ttgtactccg catacatttc tgtccgaaaa gtcgtttttc ctgcatcgaa cgcaaccaga
     8101 acatgagtcg gttgctcttt ttgcataaca ttttcaaaca tcgtattgaa tgcatagatt
     8161 gcatttgtat gaagaccgtt tctatttttg aaacgttcaa gagaattgtg aagcgcaaag
     8221 aatgcgcgaa aagccacact gttcccatca acgagtaata atttattttt tgtcatcttc
     8281 ttaactcctt actgtaaagt tcttattcat tctaacaaag aatcctgcgg aatgcgaaaa
     8341 gaaaaagcgg gaccatacca aataattcgg tatagcccca actgatgaat ttttttcttt
     8401 tttagttgtt ccgtccaaag attctcagaa acgccaagaa caagttgata aagtccaaat
     8461 acagcttcaa tgccatgaat acagcaattc cactatgtgc tgttccgttt gtctggacat
     8521 agatattacg gatgctttgg ttatcataag ctgtaatccc tgcaaagatg acgactaaca
     8581 gtagggagat gaacagctca actacgctac ttctcaaaat gaagatgttc aagactagtg
     8641 caatgatcac tccgattaaa gctgcctgtc ctgctcttcc aaccccgcta agatctcttt
     8701 ttgttgttac tccgacaaca gacatcccca tgaacatcaa agtagctgtc aagaaagctt
     8761 gcgttactgt tccaatatcg tagtatgcaa gagtaaccgc taatgttatg ccattcaaaa
     8821 gagagtaggc aataaatcca ccgattgcta agctcggatt cttttgtgct tttacgccta
     8881 gaaccattac taaaatcagt tcaacgatcc acagacccac aaagcctagt ggaaaattat
     8941 tgataaaagt tgctaattgc aaaggaaata cccaaagagc caagtaagaa gtgactgcac
     9001 taagggcaag tcccatacct aaaaatccat aaacttttgc ataaaaacga ttgatacctg
     9061 acattgatgt acttggatta ttcattcaat tcaccttcac tttcgattgc tatttccaat
     9121 tcaggaactt gtggcggttg gaccataacg acagcatcca acacacgatc ttcgttcgta
     9181 ttctccgctt ctacagaaat cgtcaccaca tccttcatct tatctacttt tatgacctca
     9241 aactgaaaag tcagtgtttc ataatggaaa accggttcaa taaaattgac agaaaaattg
     9301 acaacatgtg atcctggtcc aggaagatgc tttgaaattg cacttgtgat gatccccatc
     9361 agcatcactg atggaacgat tggctgttga tattctgttt tttgtgcaaa gtcatgttgg
     9421 atatacaaag gatttgcatc attcgtcaat cccaaataca gcaaaagctg gttatcttca
     9481 atagattctg ttaaggataa cgagtcgcct tcctcgattt catttattgt ttttcctagt
     9541 tttctcggct tacctatgtt caagatagaa accctccagt ttatttttaa tcatacatta
     9601 ttgtaccaaa agtagcgtaa aaggcaagtt ctgctccctt gatttaacag attcctatag
     9661 tttacagagg tcgtgaaaaa gctgtcctgc atcaaataat aagaacagcc aagaaaaaac
     9721 agcttctcat atttttcatt tggctggact tattaccgca gatgcaagct tttgaacacc
     9781 gtttatctga ggtcgtgaaa aagctgccct gcatcaagta ataagaacag ccaagaaaaa
     9841 cagcttctca tgtttttgat ttgattgggc ttattaccgc agatgcaagc ttttgaacac
     9901 cgtttatcat aagttgcaaa aaagggggct tggcaagatt tttgccactc cccctctttc
     9961 tatttttata aggacactgc agtaattgta ttacagtaca tttttcgtcg tgctgcttaa
    10021 aagtttttca tacgcttgtt caaatttttg aacatctcca gcacccatga agattactac
    10081 tgcatcatga taatctaaca atggagatac attattttct ttgatgactt ctccaccttt
    10141 tttgattttc gctccaaggt cttcaatctt aacgtttcct tgttcttccc gagccgaacc
    10201 aaaaatatcg cataagtaaa ctttatcagc taaatcaagt gcttctgcaa attcatccat
    10261 caatgcaatc gtacgcgtaa acgtatgggg ttggaaaact gcaattattt ctttatctgg
    10321 atatttttgg cgtgcgccat caatcgttgc tttgatctct gcagggtgat gcgcataatc
    10381 atcgactacg gtcatgtctg ctacgatctt ttcactgaat cgacgtttta ctcctgggaa
    10441 agtcagcatt tcttctgcaa cttctttcag atccagtttt tcgaaataag ctactgcaat
    10501 aacacctaaa gcatttaaga tattgtgttt accgaatgct ggtacagtga agtgcccaac
    10561 aaattcatcc ccatgataga catcaaaagc agaaccagaa gttgtgcgtt cgatgtttcg
    10621 tgcttggata tcatcgtttt ctgtgacacc ataataataa atcggcacat ttgcttttaa
    10681 tttgcgtaaa taagcatcgt caccatacgc aaaaatagct tttttcactt gtccagccat
    10741 tgtttggaaa gctgtaaata catcgtcgat acttgtataa taatccggat gatcaaaatc
    10801 gatattggtc atgattgcat agtctggtga ataggcaagg aaatgacggc gatattcaca
    10861 ggcctcaaat gaaaagaatt ccgcttgcgg atcaccatgt cctgttccat ctccaattag
    10921 ataacttgtt ggacggacac cactcaatac atgagagagc agtccagtcg tacttgtttt
    10981 tccgtgagag cctgttacag cgatactcgt ataattttgg ataaaatgac caataaagtc
    11041 gtgatagcga atcacttcca aacctaattc ttttgctcgc tggatttctt catgtgaatc
    11101 aggaaatgca tttcctgcaa tgatcgtcat ccctggtttt acattatctg cgttaaatgg
    11161 cagaatcgtg atatttgctt tttctaaatc tctttgtgta aagaaatatt tttcaatgtc
    11221 tgatccttgg acatttagac cttgttcatg taaaacaaga gccaaggagc tcattcctga
    11281 acctttgata ccaacaaaat gatacaattt attttgattt tccatttgaa tcctcttctc
    11341 ttatctagcg tccattcttt tagctattac attcaacacg ctcctcatta tcttataaac
    11401 gattgaaaat ttcaaccaaa tttatttgga aaggaggatt cgcttgctaa aatggtcaag
    11461 tacgggaaaa atccgttttt attgactctt taattctggt gtaagaagac caaacttcct
    11521 ctgatatatc aatgtgagga ataaaatctt ttcctttcgg accatagccg ttctcatcgt
    11581 catggagacg ttccatttct cctaatacta gcgcatggaa atattcctta ggccattgat
    11641 gattcagttc ttttgctgta aagatgatct cttcagaaaa gtgaaggatt tccggcacaa
    11701 atggcacagc attcgcgaaa atagtttgtt cattttgcca tactttctca aaagttgctc
    11761 gagtcctgcg ttgcagtgtc ggatcattca tcaataagat tttttctggt acagctccta
    11821 aggaatgaag aatctccagt gaaaaagtgg cgttctctcc agaatttgta gacttcgatt
    11881 ctatcagaaa agccttatcg ggcaaatcat agacttcttt tagatactga cgacagattt
    11941 cactttcact catgccatct tcaaaatgaa atccttgctt ttcaaaattc tcataaagga
    12001 ttctagtcgc atgccccacc cctccgacca aaaacaattg atctacttgg ccatttaaat
    12061 acatttgtgc tgctttatca gctaagatag gaagacagtt tccagctaaa ataagtgtag
    12121 gtgccgtcag ttcctcaaaa tttccctctt caggtgcggc taaaaaattt aatagctgat
    12181 tccagtcttt tagattcctc tgctcacttt tttccaaagg ccttctatcc tttcatctga
    12241 ttttttgtgc ttcattaaag tcttctcttt ttgtctcaat ccatttgatt tctccatgac
    12301 atcttccaca acgatatttg cgtacatcga ttcttctctt gcggcagatc gtggcagaac
    12361 aatcttggca tcgataatac tccatcttta tctgtttctt cgtcaaggct ggtgcatagc
    12421 gcaaccctcc tgtttttttc aaaagatact tgaaatccat atctttatgc tgataccctc
    12481 ttccagctag atgaagatga taatggcaaa gttcatgttt gatgattcct gcaatcgtct
    12541 cttcgtctga ctcttcaaat agcttcgcat taaaatctaa atgatgatcg tttaaatgat
    12601 agcgcccgcc agttgtacgt aagcgtggat tgaacattgc ttggtgcttg aaaggaagct
    12661 ggaagctttt taatgatatc tcttctacta actgctgcag ttcttcttgt gtcaattttt
    12721 gttactcctc attgttactc ttcataaggg gaaagcatcg tcaaactgat tctgcctttt
    12781 ttcgtatcga cttgttctat ccaaactgtt acgatatctc ctactgacac gacatctgta
    12841 ggatgcttga cgaatttttt gctgagtttc gatatatgga caagtccgtc ttgcttcaca
    12901 ccgatatcaa caaatgcgcc gaagtcaatg acattacgga ccgttccagt caattccatc
    12961 cctggtttca agtcttccat actcaataca tctgtacgta ataagggcgc tggcatctca
    13021 tcacgcatat ctcgtccagg ttgtgtcagc ccagctaaaa tatcggctaa cgtttcttct
    13081 ccgactgata ggctttcagc cagtttctct acggataatc gtgtcaattt ttctactgct
    13141 tcttctgtac caagctcttt ttctgaaagt tggactgaag ttaatatttc ttttgcaata
    13201 gaataacttt cgggatggat acctgtgttg tccaaaatgt ttttcccacc agggactcgc
    13261 aagaatccga ttgcttgttc atatgctttt ggtcctaagc gtggcacttt tttcaattga
    13321 gttcgtgcgg tgaattctcc gttttcttca cgataaatga caatgttttg ggcagttgtt
    13381 ttattcaatc ctgatatatg ctgcaagagc tgcgggcttg ctgtattcac atccacacct
    13441 acttggttaa ccgctgtttc cacaacaaaa tccaattgtt cagccaaacg tttttgagaa
    13501 acgtcatgtt ggtactggcc tactccgaca gctttaggat cgattttgac taattcagct
    13561 aaaggatctt gcaggcgacg ggcaatagaa acagcacttc tttcttcaac ttgtagatgc
    13621 ggaaattctt ttcgtgcaat ttcgctagcg gagtaaacag aagcgcctgc ttcgttgacg
    13681 atagcgtaat aggttttatg gtcggcggat ttcaactgct cagccacaaa aagttcagat
    13741 tcccgactgg ctgtaccatt tccgatagct accatatcta cttgatattt attaattaat
    13801 tggataaaag ctggtccagc agcttctctt tttgcttgcg ctgcaggttt atgaggataa
    13861 ataacttcga ttgctaaaac tttccctgtc gcatctacga ctgctagttt acatccagta
    13921 cgataagccg gatcaaatcc caggacaact ttacctttta gtggcggttg caacaaaaga
    13981 ttacggaggt tttcgccgaa aatagcgatt gcttgctcat ctgccttttc agtcaattca
    14041 ttgcgcagtt ctcgctcgat tgctggctga ataaagcgtt tataactatc ctgataggct
    14101 tctctgacga atgaagaaga aggagaggca ggatttttga ctatttggcg atccaagtaa
    14161 gctaaaattg ctgcttcatc tacttgaaag aatactttca gaatatcttc tttttctcca
    14221 cgattagtag ctaatattcg atgagaaacc attttatgga cgggttctgc aaagtcataa
    14281 tacatttcat atacgccttt ttcatctgct tgctcatcct tgacattgct gacatacata
    14341 cctttgttat acgtataaga ccgaatccaa gtccgaaatt ttgcattatc gctgacttgt
    14401 tcagcaataa tctcatgagc accgtgcaac gcttcttcgg cagaagagac ttctttttct
    14461 ttgtcaatat atttttctgc ttcagattgc acctctccat ctgtaagctg catcagccac
    14521 ttcgcgagtg gttcaagtcc tttttcttta gcaatcgttg cttttgtccg tcgtttttgt
    14581 ttgtatggac gatacaaatc ttccacttgt tgcattttca cggcttgggt gatttctgct
    14641 tctaactcgg gagtcagttt cccttgttca tcgattagcc gaatcacttc agttttacgt
    14701 ttttctaagt tttcaagata ggcataccgc tcttcgattt cacgaatctg gacttcatca
    14761 agacttccag tcatttcttt tcggtaacgc gcgataaaag gaacagtgtt tccttcattt
    14821 aacaaattaa gtacagtagt caactgctta ggacgatagt ctgaaagttc tttttgtaca
    14881 agctgaatga tcgtctggtt caaattttcc gtcattaaaa tgcctctttt ctgctaaaaa
    14941 ttgcttcttt attctatcat actttttatc ctgggttaaa ctactgaaag aaacaatatt
    15001 ctctataaaa ataaaaagag ccgcaacaaa gtcacagctc cttcattctt tccattagtc
    15061 agattccatg ccaaggtcct tcgttgttct gatattcaat atcttgaaga ataccttcct
    15121 tgtcaaaaac aaccccatgc aatgaccctc cgaatacagc cccaccgtcc attccgattt
    15181 tatgatcctg aatccagagg tctgttgtct gcatatctcc atggagcatt ggtgtaatcg
    15241 tatgtccaaa tacaattgtc ttacctgtat tgtttttccc ttcatgaaat ggttccctta
    15301 tccaaataaa gtctcttaga cttgtcttac gccagtcttt ctttgtcaaa tccacaccgg
    15361 catggacaaa aagataattt tcccattcat aatacaaggg gcgctggact aaaaatttaa
    15421 tcagttctga atatctcgat tggatcatca tcgagatttc agttggggag tattcctccg
    15481 atgcccctgg gtgaatcagg ctttcaaacg tttcttttcc accatttctc atataaggcg
    15541 cataccagtc ctccggtgat tgcagaaatt gcaaaaaata ttcttcatgg tttccccgga
    15601 ggtaaatggc tcctgtttct tcgactaatt gctttcctaa taaaaaacat tcttttgtac
    15661 gaggacctcg atcattgaga tcccctaata aaacaagctg atggatcttt ggatcataat
    15721 agtgaataag tttttcaaaa agctcatgtt tgccatggac atccccaata gcataaatca
    15781 atgatttcat gtggatcacc atcctcaacg ctagtatagc gcatattgga aatggtcgac
    15841 aatcaaaact attgttttct atttaattaa agctgtaaac cgcagttctg cttcttctgg
    15901 agagattcca tggtcttgaa catagcgatt gcgcggatgt ttcgcacact ctatgctaca
    15961 tgctcctaaa tgtttttctt cattttcaat ggaagcaaga atctgacgat tgcactcagg
    16021 attgctacaa ttgatatatc gttcacatgg tgttccgtca aaccagtcgc gaccgacgat
    16081 ttgtttatct acttggttga tatcgacagc gatccgttca tcaaacacat acatcttgcc
    16141 gtcccataat tctccttgga cttcaggatc cttaccataa gtagcaatac cgccatgcag
    16201 ctgtccgaca tctttgaatc cttcacggac aagccaacca gaaaacttct cgcaacgaat
    16261 gccaccagta cagtaggtca ctacccgttt ttccataaat tcttctttgt gctcgcggat
    16321 ccagtctgga agctctcgaa aactgcgtat atctgggcgt acagcccctc gaaaatgtcc
    16381 caagtcatat tcataatcat tccgtgcatc aatcaccact gtctgttcat ctaaaatagc
    16441 ttctttaaat tcttgcggtg aaagataggc acctgtcact tggtttggat caatgtcctc
    16501 ttctagcttt aaacttacaa gctctggacg gtatcttaca aacatcttct taaaggcatg
    16561 tccttcgctt ggatcgatct tgaacatcgt atcagcaaat cgaggatcag ctttcattgc
    16621 tttcatatag gcagtcgtgt tttcaacggt tccagacaca gtaccattta ttccttcttc
    16681 agctactaga atacgacctt taagatccaa agatttgcaa aaggctaaat gatttttggc
    16741 aaattcctct ggttcttcga tttttgtata attgtaataa agcagtactt gataatccat
    16801 gtatttcttc cttccttctt ttcgttccta aacagtataa agaaggcgtt cgcatttgac
    16861 gatttatttg cttgtgtata attacgcatg gaaagttttt tgtttacata atattattat
    16921 ttgaattaaa aagacttcgc tttcctgttt cgcacagaaa aacaaagtct ttatttctat
    16981 tatttacctg taaccgcaaa gtagttgcca tcgggatcag caaagttaaa gacatattct
    17041 tctccaagct gtaccatatc acctaatgtg actgattgcg tctgcatttt tttatatagt
    17101 ccaaagatat ctttactgta aaacatcaaa gatggggcac tagtagctgt gccaggtgaa
    17161 tgggactcaa caaatgctcg atcatataac agaaatgagg tttgactgtc ctcaactcct
    17221 acttcaatta taagcgtccc atccatttct tgacgatctt tttccgaaag ccaactgctc
    17281 tccaaaacgt tgcatttttt tctacatcgt ccacataaag catgattttt atttcattcg
    17341 taaacatctg tttcctccta aacgatctgt tctaacattc tcaactttgt cagtttacca
    17401 taggaaaatg ggaaaatcta cgaaagctct caacagaatc gtcctactta aatacgcttt
    17461 tctttaataa taaataagtt ggttgttttt gttatttacg ctttcacttt tcggaataaa
    17521 taatgtgtat ttatatggaa taaataaaaa aagaggtcgt gactaaaaac aagtcacaac
    17581 ctctggtaaa cggtgttctc ctgacaactc ctcggtcaca agacacaata ttcaacaaac
    17641 atgagaagct attttttgaa attgctcttt gtgactcaga gtgaaacggc aggctcacga
    17701 cctctttctt aaacggtgtt caaaagcttg catctgcggt aataagctca accaaacgta
    17761 aaatatgagg aactattttt gtttggctgt tcttattact tgatgcaggg cagctttttc
    17821 acaacctctt attcacggtg tttcagaagt agcttctcgg aaataagccg aaatttcaca
    17881 aaaatttgag aaactatttt taggaaactt cttcttattt cttaaagctg accacttcta
    17941 tcacaacctc tttttgacta tgcattcaca tcgatcactt tagaatcatc attgatgaca
    18001 tcgcgatcta aatctttgat gcgtgcactt gtcacgatac cagctaccat gctaccgctg
    18061 acgtttactg ctgtacgtgc catatcgatt aatggttcaa cagagataac taacccaacg
    18121 attgctactg gcaagttcat tgcccctaaa acgatcagtg aagcaaatgt tgcgccccca
    18181 ccgactccag ctacaccaaa agaactgatt gttacaattg ccacaagcat caaaataaat
    18241 tccaaactaa agacattcac tccagctgct ggtgcgacga tcgttgccaa catagctgga
    18301 taaacgccag cacagccgtt ttgtccaata gacaaaccaa aacttgctga gaaattagct
    18361 gttgcatcat ctactcctaa agcttttgtc tgagtctcga tattcaatgg caaagctcca
    18421 gcacttgaac gtgaagtaaa cgcaaagctc aataccggaa aggctttttt gaaatagttg
    18481 attggattga cttttgcacc aatcaaaatc agtgagtgga caatcaaaac agcaagcaat
    18541 gctacgtaag aagcaacgac aaatttacct aaattgacga tagctgcaaa atcacttgtt
    18601 gccaaaacgt ttgtcatcaa agcaaatact ccataaggtg tcaaacgcaa aacaagcgtc
    18661 acgatccgca taatgatttt atacagactg tcgactaaat ctgaaaagaa tgctgcttct
    18721 tttggcgctt ttcttttcac gcctagataa gcgattccga caaatgccgc gaaaataacg
    18781 actccaatcg tacttgttgc acgactacct gctaaatcag caaaaacgtt tttagggata
    18841 aaggataaaa tctgctgtgg aatcgataag ttttcaactg tttcctgacg tgttgccagt
    18901 tcagagattc gtgctgtttc tgcagctcct tgtgtgaatt tagctccttg gagtccgaac
    18961 agcattgttg aaccaattcc gatcaatgct gcaatagctg ttgttccaag taaagaagtc
    19021 aatacagtcg cactaatttt ccctagcttt tcagaaactt tcatttttgt gaatgcacca
    19081 atgatcgaaa taaacactaa aggcataact agcatttgca agaatgatac atatccttga
    19141 cctacaatcc cgatccattc cattgattga gtaacgactt tgtcgtctac accaaagatc
    19201 aattgaagca gaccgccaaa gacgatacca actcctaatg ctgtaaagac gcgtgttgaa
    19261 aattttgtat ggcgcttttg catacgatag aacacatata ataatgccgc aaaaactaga
    19321 accactaaaa ctgtgatgag tgttgtcatt tttctcctcc tcatattatt aatgacaagt
    19381 ccatcgaagg aagtaaaggt tcccgagaat gattcttcaa ccatcctcct gaaaaaaatt
    19441 ttttcagtga cttttatatt ttagacgatt tttatcgaaa tgacattctt tttgcttaca
    19501 attagtaata aaaaaacata cctgtgatag ctttaatcta tcgcaggtat gtttttactg
    19561 tctattcttg tttttctgat tgaaaattca attttacccc aggctgatct aaggcgatct
    19621 ctgtcacctc ataagacatt cggcgtacga tatcgaataa cggtgctact agctcgtcca
    19681 cttcttcttg tgtgacatca ttttgacctt caattttccg attgaggata tgattgatct
    19741 gactcacact tccagataaa acatatgtat caaacactaa acggaactct aagcgtgctc
    19801 ctacgatgct atgttctttc ggatagtttt cgatcgtttc ttttaaaggc gagaagccta
    19861 ctttcaaatc tgtttctact tcttggttcg gcaatctcat atcataatga aatgcttcta
    19921 caacttcttt ttgtcttctg atttccatat aattttatca ctcctttatg aaaattataa
    19981 catatctcct ttgaaagggg atactacttt ttaatgatgc tctgcaaggt aaggatttca
    20041 ttcagatttc ctaccacata atctgccggg atctcaccca gaaaatgatc gatgtcgtaa
    20101 agacaagtag cgactcctgc atttttccct gcttcgatat ctaaacgacg atcgccgatc
    20161 atcatcgtgt ctgttctttc taaatgaaaa gtatcgatca aataatttaa agatgctgga
    20221 tcaggtttac gaggaaagtc ctgatcgatc cctactactt cctcaatcag atgtgccaat
    20281 cgatgttctt ttaacaggcc ccatgttgat tctgtcaaac ggtgggtcag gatgaaatga
    20341 cgaccgccgt tgtcttttag catttccagt acttgtttcg tttctggaaa tggacgggaa
    20401 acttccgtac tttctttttc atatcggtga aataattcat taaactctct ggcattcaag
    20461 tttgattcat tgatcaaata actagtagaa tactttttca ttttgaagta gatttctttt
    20521 ttgtccatac tgataccaaa atcttttagt gcttgccatg caccatctac cattgcggga
    20581 tacgtatcaa acaatgtacc atcaaagtcc catatataat ttttcatgtt ccttttgttc
    20641 cattcctttg cttctttttt ctatctctca cttttagata aaggtgttct ttaaagtatt
    20701 tttttgtcca gaattgtcaa atcatctgct aatgtatact ggattggttg tgcatttggg
    20761 atatcgatcg tatccatttg atcttcagga acatcttcta aatattttgt cagtgctcgg
    20821 atgctattac catgacccac aactagtaaa tcttgccgat cttttagttt tggtgctaga
    20881 tgatcttgcc ataagggaat gatccgattc actgtcattt ccagactttc tccgtatggg
    20941 atcaaatgcg gatccaagtg atcatatctg cggtcaaatc tattttctgc ggttaacggc
    21001 ggaacttccg tatatcctct gcgccatgtt tttacttgat cttttccgta tatttctttc
    21061 atcttatctt tgtttttacc cacaagatcc ccataatgcc gttcatttag acgccatgtt
    21121 tttattatag gaagccataa ctgacccatt tcttctagtg cgacttgtcc ggtaagaatc
    21181 gcacgtttca aaacagaggt ataaacggca tcgaattgaa gtccggcttc aagcattttt
    21241 tgaccggcaa ctgctgcttg ttcttttcct ttttctgtta aggcgacatc tagccatcct
    21301 gtccagtaat tttcaaaatt tgcttcgctt tcgccatgac gtaataatac taatttcatt
    21361 cttttctccc ttaaacattc gtttcgaatt ttataccaca tgttttgcat gtcacacgta
    21421 ttcgtccttt tcctttaggt gcacgctgct tttgatgaca attcgggcac tcaaaaaaag
    21481 tataactttt tcgatccatt ttgacttgct ttttgttttt tatctgatga tatttttctt
    21541 tgactctatc aagatatttc aaataacgtt gattctcatt tagtctgggg tagatttttt
    21601 ttgataggaa acgaaatatc atccatccga aaaagacgat gaacaacaat tgtccgattc
    21661 tatagggcaa ccaacgactt agcaaactaa agacaagcgc agcgatcaat agaccgcgat
    21721 tcaactgatc aaatttcgca tatcttccct gcataagttt gtgatagttc tgctgaaatc
    21781 gtattagcct ttctaaccat gtttgcatca tattatctcc tccatcatct aatatttatt
    21841 atgacaaaaa aatccaaaaa gaaaaagcct taattggcct tttccattgc aacttggtga
    21901 aggacattcc gatttctggt aaagtcaatc acaatcactt tctttttatc ttctctgttg
    21961 actactcgaa atgtgtccgt caatggaaga aacagcttct tagatggtag ctgttgcata
    22021 cgtataaaac gatggatcat cgtaatcaat tgaggaaatt gaatagacgt gtgaagcaga
    22081 cctggatatt ttttcaacag ctgtgtcgtt ttgatcagat tccgtttttt tactgatcac
    22141 ttttaaaaag ctttttagaa tcttttgctg acgtctaaaa atagaaaaag aattttcgtc
    22201 atctatttga taacgcattt gtttttgcag ttctgctgca gtcagatgga tcttcccatc
    22261 gtttgtatcg atcattaaac cttctggaaa cagttcaacc actgctcgat cccaagattc
    22321 aagagaaagc tcgatataat cttttactgg taattccaat tctaccttaa aaaagtgcaa
    22381 aacagccgac agcccttctg cttgatagat ttctcctaat gttagcccct ctagatggaa
    22441 ctctggtgaa aaatgcagca atgttttatt ttcctgcaat tgaacatata gaaatgtaga
    22501 tcgctcaatc agagtatctt ttaaaacaac taaaaaattc atttttctcc cccttttttc
    22561 atcattttta aaatagcata ttttgaaaag aaagtctgat gcttagagta gaatttcata
    22621 aaaatttact atttcataag tattcacgag aaaaatacaa acaaaaaagc ggaaataacg
    22681 ctcatccgcc ttcttgtttg tgtttatttc ctcttcaaac gcttgtctat gattcagact
    22741 tttctctatc gttattttca tctaaaagtt cttgcattcc gataatttct cgatattgat
    22801 gaatcttagt gataccaatg actttgtcta catcaatact aaaacaaata ccattacctg
    22861 gaatatcgat gttgatgcct tgaccaatcg catccattac tttattcgtc aaactatcgg
    22921 gtactaatgt caacacaata tctttttctg gcgaaatgtc caaacctaaa aatttccctg
    22981 aatcatgtac accagaacct cttccatgta agatcgttcc gcctgctgca cctgcttctt
    23041 ttgaaaagtc gataacatct tcgcccattc ctccgtcaac gatcgtcaca atcatgtgaa
    23101 gattcaatgc tacaagatct ttcatttgct ttcccccaat ttttgcaatc tttctttttc
    23161 gattctgtct ttttcatctt ttcgtttata aatatatcct agtactaaag tggataatat
    23221 tggtgtcaat gcaatcattg caaccacacc aaacccgtca atgatcggat ctcttccttc
    23281 aatttgtgca gcgattgcca cgctcattga taaaataaat gtcgaacaca tcgggcccgt
    23341 tgctactcca ccgttatcaa aagccattgc taaaaagatc ttgtccactt ttcgtcctaa
    23401 aataaatgct agcccgtatc ctgggatcaa gaaataataa agagagaatc cgacgagcaa
    23461 acgccacata gacaatccta ctgctgcgcc gatcccaatt gaaattacca tcagcatgat
    23521 tttcgatcgg attctcccct cgctcaattc ttcaacctgt ttgatcatta catgaatcgc
    23581 cggctcagca aaccctacta agaaccccat taaaaagcca aggggaacca gtatataatt
    23641 gtaaggcaat cccgcaatag cactgcctaa atgctgtcct accggtacat aagccatatt
    23701 gactccgtgc aggaaaagga tcaacccaat agtcgttatc aagaaacctt tcataatgtc
    23761 aaagaaacgc tttttgcgca tacgaaaact aatgatattc attaaaacaa ataggattac
    23821 gataggtgcc actgccaaaa tgacttccat ggtaacgatt tcgattccat ctatcacttg
    23881 ctgccagaaa ctcattggaa gatcaccccc aaaatcatca cggcaagaat cggccctaac
    23941 gaagcaatac cgaccattcc aaaactgtca ttttcatttt tcttttgttt gatcatacta
    24001 gtcatccctc cggcaagggc caagataaat ggaacagtta ctggaccagt agtcactccc
    24061 cccgaatcaa aagcaatcgg cataaattcg gaactggtga aaaatgaggc gatcaaaacg
    24121 cctatgtagc ctatagccat caattgataa taggatagct tgaatacgac ccgaagcagc
    24181 gcaaaggcta gaaatactcc agtgccgacg ctgacgatcc caatcagcaa cactctgcca
    24241 atctctccgt cagatatatc atatacttgt tgtcccaaaa cttgcacgga aggttcagca
    24301 atcgtgatca cgatcccgat agcaaatccc aaactgacca aaacggtcaa acttttcttt
    24361 ttgatcatat atttccctac tagatctccg acttccatca tagaataatc tgctccaaac
    24421 aaaaagagcg tcattccgat catcatgatt gccgcaccaa ccagaaatga agcgaggtct
    24481 tctccttcta acggtgcaaa aacaaacgtt aaaatgacaa tcaggacagt catcggcaat
    24541 atagcagcaa tcacttcttt aaaatttttt agcatgtatc ctcctccttt actctttaag
    24601 tttaccttaa aatgaaagag ttttcgagca caaacttaaa acgttatcat ttaatttttc
    24661 tcaacataaa ataacacacg tcccaaaagt atctttttac ttggaaaaat gactttgaag
    24721 gaacgcgctt attgtgtttt tttattttac agagccgatc attccttgaa cgaattgctt
    24781 ttgcaaaaca aaaaatacga tggcagttgg taaagtagcg atgactaatc cagccatgat
    24841 cattccatag tctggtgtat aggaagcacc catagcagat aaaacaagcg gaaccgtccg
    24901 tttatcagga gattgcaaag ctactaaggg ccataaataa ttgttccagc tactcatgaa
    24961 tgtgatgatt gcagccgcgg cataggtatt tttagccgtc ggcatataca ctctaaaaaa
    25021 aatacttagt tcttttagac cgtctaatcg agcggcttct actagatctt ttggaaaaga
    25081 tttaacattt tgtcgaaaga agaagatcaa aaaagcagtg ctaacagatg gcaggatgac
    25141 aaccaagaaa ctgttgattc ccaaaaccgt tccgttcaat tgtgaaaaca ttcgatataa
    25201 agggatcatc aatgccgcaa aaggaaccat catcgatagc agtagaatat tgaaaattag
    25261 gtcttttcgt ttactttgat aaatctcaaa tccatatcct gccattgacg aaatcagcaa
    25321 tgcaaacaca gtggtcacca aagcgatcat cgctgaattc caaagggaac gagtaaaatt
    25381 cagctcattg gaaaacaaat tttgaaaatt gatgatcaag ttactgccaa tctttatttt
    25441 tcccgatgtg atatccacgg gtgtattcgt cattcccaaa accatccaga caaaagggaa
    25501 aatggaaata aatgatagga tgcttaatgc tgtatacttt aatactgacc caattgtcga
    25561 acgaagttct aatgcttgtt ttttgacgtt ggggcgttcg attgtttctt tttctaccat
    25621 atttttcatc gactttctcc tcctactttc gtttggatta tcgacaatat cacaataaag
    25681 gaaacaataa caaaggaaac tgctgccgca tatccaaaat taggcgtgaa tttataactc
    25741 aaattataga tgtattgaga tagggtggtc gttgcatttg ctggtccccc gccagtaata
    25801 ttttgtactt catcaaaaag ctgcaatgtc ccgatcgtcg aagtaatgga agtaaacaaa
    25861 atgattggtt ttaaatttgg gatcgtcact ttgataaact gctgccattt agaagcaccg
    25921 tcaatagatg ctgcttcata aatctccgaa tcaatatttt gcatgcctga aaggaaaaaa
    25981 atcatattgt atccagtcca acgccaagtg atcgaaagaa tgatcagaac tctcgcccaa
    26041 attggatgag tcaaccagcc aatcggtgtg tcaatcatac caatgcccaa caacaagttg
    26101 tttacaaggc cagattgaga aaatagactt ttcatgatca gagaataact gaccatcgaa
    26161 gtgatacatg gaagaaagat agcggttcga aacaaccctt tatatttcaa ctttttatca
    26221 tttaaaaggt tggaaataaa aagtgctaga aacaacatga tcggtacttg tacaagtaaa
    26281 tacaggaatg tattaaaaaa agctttttta aaagttgcat ctgtcaacat ccgctgataa
    26341 ttacctaacc cgttaaatgt catattgaca gggggaccac tttgaaaaga cgtgatcaaa
    26401 gccaaaacca tcggaataaa aaccatgatt gatatcaaaa tgactggcaa gattaaaaaa
    26461 gcatgcccgt ttttgaataa atctttctgc ttcattcttt tccctccaat tctatcattg
    26521 attatctaca tgaaaagaca agaaagacaa tatctgtaag gcagacgtga tcttttttgt
    26581 cttttcgttt gtctgatgaa aaagcgaaga aattcacttt tttaccaccg atcaattagc
    26641 caattgtgct tctacttgtt tttgtgtatc tgctagtacc ttgtctgtct cttctccatt
    26701 gatgattcga tgaagagctt cagctacgac tgcttcaatt gcataggtct catgtccgta
    26761 attcacttct ggtatttcag atgtccatgt tgaaaaatcg tcaaagatct tttgaccact
    26821 gtaaaattca gaagcttctt gataagcttc ttgttcattc gcgctaagca tcgtagatac
    26881 tagtccgatt tctttcgcaa gagtgcccat cagttcttca ttcgaagcaa acgttgcttc
    26941 taggaaatct ttcgctgaat cttctccagc tactcctttg atcacatacc agccagcgcc
    27001 gccaaggtta gaagcttgcg ctttttgcag attttctgga agtgcaggta ttggtgcaat
    27061 cttccatttt ccagattggt tttctgctcc ttggatcgtt gaagaatacc aagcgccagt
    27121 tgggctagaa gctactgctc ctgattgtat cgcagttact ccagcattcc agtcagaggt
    27181 ttgttcgact agatcttctt tcaaaagagt ggcaaacaat tcaagaccat actttaaaga
    27241 tgcattgtct tggatcgtga ctgtctcccc atcttcagat gtataccatt ctcctgcctg
    27301 ttgcataatc atccttactc gaccaaggtc agaaggattg acttcagcga ttttctttcc
    27361 cgtcttttct ttgacatctc gcgctacttg aatatactcg tcccacttca gattctccat
    27421 atcctcttct gtataacctg cttctgccat cagatccgta cgatagaagt ttgctgttac
    27481 gcccgaatcg aaaggaacac cgtatatttt atctcctact ttgtttaccg caaatttata
    27541 cggtgcaaaa tgatcttcct ttacaatagc agacaaatta gcgaatgcat ctggataaga
    27601 agtcaaatag ccttgtatcc tataatcttc aatcaagacg atattgggca atccttctgt
    27661 attcccgctc gctaaagcgg tgttcagttt ctgaacgata tcatcctgag acatcgtaac
    27721 aacttctatc tctgtttctt cgttctcata aacttttttt gcttcattca ctgctttgat
    27781 attgaatgtc tcatcccacg cccagacagt gactttttct tctttgtctg ctgtactact
    27841 agcacttccg catcctgtta agaaacctga tgctgccgct aaacaaacga ctccagcaat
    27901 taactttctg atgttcattt ttatccgctc cttttccatc cattagttgt ttacgtttgc
    27961 tacattcatc ataccaaaat ttgaaaacgg tttctttatt attcagatac ttcaaaaatt
    28021 tctcctaaaa taataaaaaa atacaaatca actatgacaa aaggaataat cttagcatct
    28081 agaccaaaga tcgaaatggt gaggtttctt taaaataaaa atatccttgc attttctcct
    28141 agaaataagt atttttaaat tacaagacta ttcttaaggc gggtaaaaaa atgccaatac
    28201 gcgtaattct tatcgatgat gaacagacga ttttagatgg aatgaaacag ttgatcaatt
    28261 ggtctgagca cgggtttcaa ttaataggga ctttttccag tccgctgaaa gcgcttgatt
    28321 tttctttggt acatgcaatc gatatcgtgg tcacagatgt aatgatgcct gaattaaatg
    28381 gaatcgaatt atcgcggcta ttaaaacagc aacaacctga aacacagatc cttatcctct
    28441 ctagctatga tgaatttgat atggttaaag attcttttaa agaaggcgtc gctgattatc
    28501 ttttaaagcc tcgattgagt cctgattttt ttctaaattc cttaactgcc gtttcacaaa
    28561 aaatcaaaaa aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag atctgcgaga
    28621 atctttcaca gcagattgct ggacaaactc gtccattgct tgaccctctt ctttttgaac
    28681 acaccttgtt tttctgttat ataccgaaaa acggccattc agttcaaaac aaggctctaa
    28741 acaaaaacag tcgatcaccc attcattgat ggatagcgac gattcgttta ctatttatcc
    28801 tgtttctacc ttttcggaag aatccggcta tgtaatcaat actgattcca aagatacact
    28861 taggaagttt gttcaaaagt ttcgtcaaga agtttttcca ggtgattttt ttgttgtttc
    28921 tgattctttt gcaatccaag atatcaatga aatattccaa gagatcagaa attgcaccaa
    28981 aggacaacgt ttctatcata agggaaagaa ccttgcttat cagcatgaac ttcttccgtt
    29041 attaaccaac cacgagcagc aagcaagtag ttatttaaca aaaatcgttc ataaagacta
    29101 cgctttagct gtcaaagaac ttgagaattt cttcacgacg atattggaac agcctattga
    29161 tccagcttat ttgaaacatg aggcaatcaa tcgtttctat gcattactca acgctttggc
    29221 tgacgagcat gttgggaatg aagaactttc tttgctcaag atcgcccttc cttctttatt
    29281 agccgatgct gagtatttcg aagatttttc tattcttgtc aaagaaacac ttgagaaact
    29341 cacccacttt tctgcaaatg caaaaaaaga caatttcgat atcttgactt tgatctacga
    29401 gttcgtccag gaaaattatg agaaagaaat caatttacaa cttttatccg aaaaatatca
    29461 tttttcatat agctatctct cagccatctt tactgaaaaa tttggtatca acttttcaaa
    29521 atatctaaaa aaagtccgta tcagtaaagc caagctcttt ttgacggaaa cacggcttac
    29581 tttgacggaa atctgtgaga aagtcggcta taccgaactg ggttatttct ccagagtttt
    29641 taaagaagag acaggaatga caccttctca ataccgtaaa ggaacttttg taaaatgaga
    29701 aaaaaaatca aagagcatga actctttgcc aaacttctgt tcattttgtt catcggtctt
    29761 tttctgcaag ccatcgtcat ttctttgttt atttatcatc gatcaaggga tgcctatatc
    29821 cagctgttca atcaatcgaa tgatgtggta ctaaaaaaga ttcaatctga atttgaaagt
    29881 ttgaatgata caatcgagaa tacattagcc gcttttgatt ctaatccagc tgtcaaaagc
    29941 tatttttcca atgatccagc ccaacatatg gaacagcttc agcaactaag aacaatccaa
    30001 aaaatgaatg actctctttc aaaaatccat ccaatgatcg attacgatgt attgatcttt
    30061 ggagaaaatg gaagaacttt tgtcggaaac gacatgctca ctgccgtatc tgccgattct
    30121 ttttttcaat ctgcgattgc tcagagagtg aatgaacgtg ctgctgatac acaaatgctt
    30181 tttgcacacc acggattgac tcttcgcgat aagaaagcac ccagcgtctt ctttgtcaaa
    30241 aaactgaaaa atacattgaa tcacgtttac ggatatgctg ttttatcgat ctcatccaag
    30301 caactagcga atctttttca atctgtagta aatcctgaga tatcgaagat ctccttcatg
    30361 aacgaagaac agacgatcat cgcatcaaat gaagaaaata caattgggag ccgatcaatg
    30421 attttcaaaa aactaacagt aggtgaaaca acgatttcaa atggacagca gttgacccgt
    30481 cttcctctgt accgccagga ttgtacatta ctcagtcaag tcgatcttca ttctttatct
    30541 aaccaaatgg gcgtgatttt tccgattatt ttgtataata tcgtgacact tgcttttgct
    30601 ggctcattgg tatttatgta tttggatcga cacacaaaat cgatctaccg cttgattgat
    30661 tcgctaaaac ggctagagga agatggctta aaagctactg ttcctgtaca aggaagccat
    30721 gaagtccaat tgctagggaa tacgatcaat caattgctaa gtaatgctca gcatcattat
    30781 gaacagacga tccaagacga aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca
    30841 cagatacagc cccattttat ttataatacg ctgactagct tgaaattttt gatttggcag
    30901 aaaaaaacag atgaagcagt tcaaggaatc gacagtttca ttactttact aagaagtacg
    30961 attgggaaaa aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt acaaagttac
    31021 atcgatcttt tgagtatgcg atatggcgaa agaatctcgg ctaaaataat gattcctgat
    31081 gaactgcagt cattatctat gccgaatatg atccttcaac caattatcga aaacgcctat
    31141 cttcatgctt ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat
    31201 cagcttcttt tattcgaagt gattgataac ggcgatggct tcgatactga aaataccaaa
    31261 caaaaaaatg atcatttttc aggaatcggc atcagccatg tagatgagcg gatacgccta
    31321 atgtatggcg atgcatatgg cttaacgatc caatcagtga ttgggacagg aacgactgta
    31381 acgatttgtc taccgcttct tcctaaaaat caatcagaat atcaaaaata aagaatagcc
    31441 aagcgctgat ttttttattt taagttaaat aaatcaatca gtattcttga ctttcatcta
    31501 cgcgtctctt ctactgtcag cactggataa cagcccaata tttgactttc tcctccgagc
    31561 atcttgattt cttggagcgc attctctact aatgtcattg gacggtccaa caacaaatcg
    31621 atcacgaaaa aatattctcc caagctagtt ttcaatggtc gggattcaat cttacttaag
    31681 ttgatttccc gccagccaaa agctgccagc attttatgaa gcatccctgg ccgattagca
    31741 ggtagtgtca agatgacact catttttact ggagctgact gctggctggt catctttcgg
    31801 tcaccgacga tccaaaaacg tgtctggttc aactcattat cttggatatt ctcagctaag
    31861 atctccaatc caacatgctg tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc
    31921 ggatgttctg ctacatacat tgctgcagca gttgttgatt ctgttgctac taaaggaaca
    31981 ttaggataat gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg
    32041 atttttgtaa tctttgttgc tggattgcct aaaagctgct gcttgatagg aagaacgatt
    32101 tcacttttaa cttcgacttc tggatgtttg gaaagaagat cgattgtgtg atgaacagct
    32161 ccttccaatg aattctctac aggaacgaca gccagatcga cttgtttgcg aaacaaagcg
    32221 cgcaaacaaa cagggatgga agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa
    32281 gccgcctgat aagtgaatga actttctggc cctagatagc tgacgttcat atgcttttga
    32341 tcctttctat aatggtatcg atgatctttt ctggtgtttg attggtcgtg ttgattcgat
    32401 gtgtcgcact ttcttcatac caagaaagtc gttgtgccaa taatgctttt atttcttctc
    32461 ttgtttttcc aagagctaaa ggtcgaatag acgtttcatc ttgttggata cgatcaacta
    32521 gacagtccgc ttctgcctct aagtaaagaa caatcggctg tgtttttaaa aaacgccgat
    32581 tttctgcact ttggacaatc cctccaccag tcgcgataat tttgtcttca tttgacagct
    32641 ttcttaaaat atctgactcc acttttcgaa agcttttttc accgtaatag tgaaaatatt
    32701 cggcaatcga caattggata tgttcctcaa tctttgtatc taaatctaca tactcttttt
    32761 gtaaacattg agctaaccct ttaccgattg ttgtttttcc agcgcccatg aaaccaatca
    32821 gcaaaatgct agccatgagc ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat
    32881 gccggataag agattgaaac agcttcagct ccttctagtt cgcaaggtcc atcagctatc
    32941 aaagcagcta cttgcagcat cataccgatt cgatgatcgc ctctgctgga cacttttgcg
    33001 ccatgcagag gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt tatctttgct
    33061 cccattttct gtaattcttc tgccaccgca tcaattctgt tggtttcttt tacttttaat
    33121 tcttctgcgt ctttgatgat cgtgattcct tctgcttgag tagccactaa agctagaatg
    33181 ggcaattcat caatcagacg cggaatgatt tctccaccaa tcgttgcttt cttcaaatga
    33241 ctcgtcttga cacttaaatc agccgattgg ttctgttcat tgtgattcgt ctgggtaatc
    33301 gctgcgccca tctcctccaa cacatctaag ataccagttc ttgttggatt gaccccgaca
    33361 ttttttaaca acagttggct ttccggaaca agaagtcctg ctgctagaaa gaaagcagct
    33421 gatgaaatat cacctggaac agttatctgt tgaccagtta atttttgcgg tccggtcact
    33481 atgatcgttt tatcttccac tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta
    33541 tggttacggg aacgttcttt ttcaacgatt acggatgtgc cttctgcttg taatgccgca
    33601 aacagcaaag cagatttgac ttgcgcactg gctacaggca tattgtatcg gataggtgat
    33661 aaagagcggg ttcctgtcac agtgattggt agatactggt cattttcttc tccttgcaag
    33721 tcagcgccca tctcgcgtaa aggttccatc actcgcccca ttggacgttt agacaacgaa
    33781 gcatccccga acagagtagt cgtaaaaggc tgtccagcca aaatacccat caataaacgt
    33841 atggtcgtac cggagtttcc catgtcaatt gattcggttg gttggacaaa tgagtggcta
    33901 ccgcgaccat gaatcttgat cacctcttcc tcttcttcga tctcgacacc taattgttgg
    33961 aaggcatgta aagtactcag acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt
    34021 ccttcagcaa ttgctccaaa catgatgctg cgatgggaaa ttgatttatc tgctggtatc
    34081 ctaacagtcc ctcttaatcc atgtatttgt tgtaataatt gcatttatcc agcctcctat
    34141 ttgatccagc aatgataact ggtattagac ataatgattc tttttgcttg taacaaatcc
    34201 tgttggtttt taaaactgat ttgcagtatt cctaaaatat cttccctggt ctcttggatc
    34261 ttcaaattaa tcaaagaaac tcccgctttt ccgatcagtc cagttacttc tgcaataacg
    34321 ccaggttcat ctggcacatc gataaataaa tcaaaaaagg caggaatcgc gccttgctta
    34381 tggacaggca actggtctcg actttctttt gcccgattga aaaaagaaaa gatttccgat
    34441 tggttctcag tcatgatcca ttctgctatt tgtgtcattt cttgttgcca atctgccaat
    34501 cgttccaata atatctgctt attgctcaac aaaatatctg tccacatgac aggatctgag
    34561 gaagcgatcc tagtgatatc tcgaaagcct cctgctgcca gttgtttggc tcgcggatac
    34621 tcttgattga attgttctgc ctgattgact aagccagctg ccagaatatg tggaaaatga
    34681 cttagcattc ctgttatacg gtcatgttct cttgctgtaa gagttacata ttttgctttg
    34741 gttccgcaaa atagttcttg cagtttttca acatcttttt tcgtcttttc caaaggtgta
    34801 aagatgtaat aggcattttc aaatagttta gcatctgctg cctgtatacc tgatttatga
    34861 gaaccagcca ttggatgtcc accgacaaac cgaagatttt tctgctcagc ggatgtcacg
    34921 acttgctgct tcgtactgct gacatccgta atgatgacgt gatcagacaa gttgattctt
    34981 tcaaggacat caagataagc taaggttgta cggacgggaa cggctaataa gataacgtct
    35041 gcttgttctg cacctgtttc aaaatcactt gcaacgtaat caacgatctg ctgttctact
    35101 gcttgctcac gggtttttga ctgactatcc caaccaacta ctatcagttc tggattggtc
    35161 tgacgaatac aaagtgccat agaagcacca atcagtccaa gccccacgat aaaaacatgt
    35221 tgtgccatct tatttacccc ctttttagta atgtttcaag tagtcacggt actccttcac
    35281 tgcagctttc aactcaacaa gcgaatcact tgggaatttt gacaatagct ctgtcgctac
    35341 cacagtggct acaacatttt ccgcaacgac gctagctgca ggaactgctg tactatccga
    35401 acgttctaca cttgctttgt aaggttcttt tgtatcgata tcaacacttt gaagtggctt
    35461 gtacaatgta ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc cattggtcat
    35521 tccgccttcg aagccgccta gattattggt acgtcggaaa tagcctcttt cttcttccca
    35581 taggatctca tccatcactt ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa
    35641 ttctacgcct ttaaaggcat tgatactaac cacagcttgt gcgattttgg catctagctt
    35701 cgtgtcccat tgtacgtagc ttcctaatcc agctggaacg cctccaacga gcacttctac
    35761 aattcctccg atcgtatctc catttttctt tgtctgatcg atcaaatctt ttattctttg
    35821 ctcaactgct ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt tgatttcttg
    35881 aatcgagata tttccaggta tttctgcttg gattccacca agacaaatca cgtgtcctgc
    35941 tacgatgatg tcaagttctt ccagaatttt cttggcaatt gcgccgattg ctacacgcat
    36001 ggtcgtctct cttgcagagg accgttcaag cacattccga agatctcgat gatggtattt
    36061 cataccgcct accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg
    36121 gtctttttca ggcacttctg ctacagacat gacagttgtc caatttttcc aatctttatt
    36181 ttctaccact aaagtgacag gtgaaccaat tgttttgccg tgacggatgc cagacgtgat
    36241 ccgaacacga tctttttcga tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg
    36301 agcgagttcg tgattgattt cttcttctga cagaggtaat cctgctggta gtccttcaat
    36361 aatggctgtt aattctggtc catgggattc tcccgcagtc atataacgca tcttacagtc
    36421 ctcctaagta atctttgaat gtctctgtgg gaacaggaac gatttttgct tgcccaatct
    36481 tttctagcaa gatgatattc aattggcttc ctcttgcttt cttatcatgg gttatcgcgt
    36541 ggaataattc atctggatga cttagatcgt acgtaatcgg cagatggaat ttttgaagta
    36601 tcctttctaa agcagctgtc gatccagttg gagacaagcc gattttttct gcatgagaag
    36661 tgatggctac cattccaata gctactcctt ctccatgact gactacccca tatcctgctg
    36721 tattttcaat tgcatggcca attgtatgac caaaatttaa aataagtcga tttccttggt
    36781 caaagacatc ttcttccact acttttcgtt tgatttccaa cgtagcagta atgattggaa
    36841 cagcatgttc cagaagatct ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg
    36901 catctgctat agctgctgat ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg
    36961 gcaaagtttt tagaacctca ggatcaatca atacaccagc tggctggcta aatgttccga
    37021 caaggttttt cgctttgttt gtattgactg ctgtcttccc gccaatacta gaatctactt
    37081 gtgcaagtag cgtagtcgga atttgaacga aatggatccc acgcatgaac gtcgaggcca
    37141 cgaatgctgc tagatcaccc accattcctc caccaagtgc aagtatccca tcgctgcggg
    37201 taaaatcatt atctgctaaa aaatcgtata actcagctgc ttgttctaaa gatttactgg
    37261 tctcacccgc cggtacgatt gaaaggactg gttcaaaatt agcttgtctc aattggaaca
    37321 tgattttttc tgcatatagt ggagcaacgt ttgtatcagt gatcacagct actttttgag
    37381 gtctccaaat cgtttgaagc catgatccag cctgttctag tgcgcctttt tcaatatgaa
    37441 tatcgtattt ttggttatca acatgtacag taagcattcg tttcacttct ttcttttaaa
    37501 aagctggaat taatcgagtg tctgaatctt ttccattgct tctttcatga tcgcaacttc
    37561 tttcatcatg cgtgagtaag tcttttcatt caacgattgg ggaccgtctg accaagcatt
    37621 tgcaggatca ggatggatct ctacgatcat gccatctgct ccgcttgcca cacccgcacg
    37681 agccattggt ggtacaagtt cccaaattcc tgtaccatga cttggatcaa caatcacagg
    37741 gaaatgagtt aattttttca tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc
    37801 tgtttcgtaa gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt cttcgactgc
    37861 aatgtattct gcagcattaa gccattcatt gatcgtaccg gaaattccac gtttcaatcc
    37921 aacaggttta cctgttttac cgacagcaga tagcagttta aaattttgca tattacgtgc
    37981 gccaatctgt aggatatcgc tgtactcagc caccatatcg atatgccctt catccatcac
    38041 ttcagtaatc actttcattc caaattcatc agccgcttga cggatgtatt tcaatccttc
    38101 ttcttctaaa ccttggaaag catatggaga agtacgtggt ttgaaggcac cacctcgcag
    38161 aatttttgcg cccccggctt tggccattcg cgcacattct ctgatttggt ctaatccttc
    38221 gatagaacaa ggaccagcca tcgtcacgaa actgccgtca ccaattttta ctccgtctac
    38281 atccacgact gtattgccag gatggaattc tcgagaagtc aatttgtatg tgtttaaaat
    38341 acgcacagca ttttctactc cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt
    38401 atcacctttt agtccaatca ctactcgttc tgttccttga tcgattgaga ctttgaatcc
    38461 ttctttttcg atatgatcga cgactttttt tatttgttct tccgttgctt cttttttcat
    38521 aattactatc attgtttttc ccgctttctt ttgtttactt atctatcttg gctgattttc
    38581 tctacttttt caaggtggtt ttacattaat tcctactaaa tgaccgtgtc ctgttgttct
    38641 cattttcgct ccttctttcc tacttatgac ccagataatc aacaaaaaaa gatgtccctg
    38701 taggaaattg cctctacagt gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat
    38761 aaaatagcca ctatagatga atttctctac gtggctgtaa gtcgtgttta aaaatcttta
    38821 gccatcatag atacaaacct ggtttgtttg tacccacgaa gtcatgaata caaacctatc
    38881 taaaatagct aaagtaatac gtattcagtt ttgtaagttt tctagatttc ataaaaaatc
    38941 gctccaaact gatgtaagaa ttgtacctag tatagacata aaaaaatcga ctgtcaatca
    39001 tttttatgaa acttttttta attttctgat agttaacttt gtttttcaga aaaataacaa
    39061 ttgattttat tgaagaaaat agtcatgaac aaaagtatct gagaactttt ttactctctt
    39121 gttcatgaca tatagaataa agcagctttg tcttgtaaac tccgtttttc aaataataat
    39181 ctaaagattg ctactgaata ataaagccga tttcatggtc ctcttcgctg ctgattcggt
    39241 aaggtgactc aacatccgct ccccagctat tgataccccc gactcctctt acttttgcaa
    39301 aaatcgtaag aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt gcctgttcca
    39361 gttcttctgc tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc
    39421 gcaacgaaaa atcttcttga tctttatccg tattatccaa cgtttgattt cttgttagtt
    39481 ctacccattt cgtttccata tgcatgccgc attcttgggg aacaaggtat ggggaaacgg
    39541 ggagtcctgc cactttataa ataccttcga caccaccagc catccgatcc ggatatgttt
    39601 cccctgatag gccttcatac tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac
    39661 ccaatgcggg taattccggt aatccttttt gtccataata atgcatattg acaagcattt
    39721 taccctctcc agcgatttgg taggaaattg tcacttgggt ttttggtact gtgttgattt
    39781 ccagtgtaaa aatgatttca accgtttcag cgtattcatt acttgaatat tgattattta
    39841 ctggagcaat cggcaaatcg atctttttat tgtccaccag tacttctatt tctttgactt
    39901 ttggatacaa tccggctcct aaccacatac cggatttaaa tccaaatccg ctacctctgt
    39961 cattatcggt cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc cattcttttc
    40021 cgtttttatt gagcgattcc agtccgcctc ttgtataaga aaaaatataa tgcgtattcc
    40081 ccgatttgac acctaatgta cagtcgccga agacgattgc aactttttcc gtatccatat
    40141 gacttttatt tactgtattt ttcataataa tatcttacct cctgcatggc gggtttttct
    40201 tgtccatctg caaaaacgat cccattccct gagaattcat agtcacttgg tttatcgtca
    40261 aagtcaccgc catagcgcat cacttctctg cctgtgattg gatcagtcac ttggatcgcc
    40321 tgatctttaa aatcccatat atagcctcct tggtacattt cataacgatc aagcaacgtc
    40381 atgtaagaat ccattccacc taacgaattc cccatgtcat gcatatattc acataagatg
    40441 aacggttttt ccgctttatt ttcaaggtag tcaatgatct cttctggtgg cgcatacatg
    40501 cagcttttta catctgaaat cgaatcttcg aatgctggat ttggaaaatt cccttcataa
    40561 tggacaagtc gatcggggtc tacttttttg aagtaatcgt tcatttgtcg aatgttttct
    40621 tccgcataag attcatttcc aagagaccaa aaaagaatac ttggatggtt ttttaatgtt
    40681 tcgaaattcg tttttgctcg atcaagaaca gccgcctgcc actgaggtac tgcacctggt
    40741 acgttgtaag aaggctcaaa tgcgcccatt ttttgccatg agccatggga ttcgaggttc
    40801 gtttccgcca tcacatggat tcctgcttgg tcgcataaat aataccaagt gatctggttg
    40861 gggtaatgag atgtccgtac gccgttgatc tttgcatttt ttatgatagc caaatctttt
    40921 tccatatctt ctatagtgat ggcgcgtcct tttttcggat gccactcatg tcgatttacc
    40981 ccgttgataa tcatccgttt tccattgaag tagatgattt tatctttgat ttcgatttta
    41041 cgaaaaccga aggaatagtc aatatactcc gttactcttt tttcttcgtc tatcgtctcc
    41101 aattcaagct tatataaatt gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca
    41161 tcgatgcgaa tcgtcatttg ctcttttaac ggatattgct gttgatagat ttccttccca
    41221 tcaggatcta gtatcgctac attcagctga ctgtttttgg ctacttgctc aaaagacaaa
    41281 tctacttcaa gtttgccatt ttgacatttg tcatctaagc gaggtttgat ccaaagatct
    41341 tctaaatggt tttgtcctcg agcaactaag aagacatctc tgaagatccc aaaaaatcgg
    41401 aaaaagtcct gatcttccaa gaatgcagct gtacttcttt tgtaaacctc cacagctaga
    41461 atattttctt cttccaaata aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg
    41521 gcatacccga taaaatgacc attcaaccaa ataaacattg cttgttctac accagcaaaa
    41581 tgaatcgaga tttgttttcc ctttagacct tcttctaatc gaaaagtcgt caaatacgaa
    41641 ccaaccggat tgtctttagc agctgaaaag atcccttccg cctctttctc agggcttgtc
    41701 gtgaaagggg gacggcgaaa tacatgtccc tcccacggat acatcgtatt gatgtaatgg
    41761 atcttgtcat aatttgctaa ttcaatatga ctaggaacag tgatttcatc aaaagaacag
    41821 gcatcaaaat cttttttata aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa
    41881 aaacgccatg atccatttaa agactgttta agggaggatg tggtttctac ttcttccctt
    41941 gttgcataac aaacatgatc gctatgtgca tttgattggt tcactctaaa aacttgtgga
    42001 tcatctaacc attttatatt tgctttcata cttttttcct tctctctcat aactttttga
    42061 aatgaagcag ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca
    42121 tcaattcggc ccccgaaaat atccgcttcg aggtctgttc ctgataaagt gcctcatcat
    42181 ctaaccccat cagatatact tcatggaacg ctggttgagc tgaagctagt atcctcccta
    42241 aaaacacaat agcttcatct tgtttaggag acacaaacat ccatgcggca atattttctt
    42301 caaaaggact cgccaaacga taataatggc catactggat caccggacga atcgtttgat
    42361 aggatataat ttggtctttt acttgctttt tttcttcagg agacagcttt gtcaaatcca
    42421 gttcataacc aaaaacagcg ctcatagctg tttctcctct tgttttcaac gaagtagatc
    42481 tgtcggtctg atgatttggt acagctgata catgagctgt gatggacgaa atcggataag
    42541 caaggcttgt tccatattgg attttcatac gctccactgc atccgtgtta tcacttgtcc
    42601 atgactgcgg catataataa ataaacccgg catcaaaccg tccacctcca ccagaacagc
    42661 cttcccataa gatttccgga taatcagttg tcaaatgttc aagtagttca taaagcccga
    42721 gcatataacg atgcgctact tctccttgtc tctgtgggtc aagggtaatc gaatagacat
    42781 ctgaaagcga acggttcata tcccatttga tgtagtcaag aggaatcgta tctaaaatct
    42841 ttctcatttg tttctcaatc gtctgccgaa catctaacct tgtaaaatcc agtacatgtt
    42901 gtgatctgga agcagaaggc attctacctg gctcctgcat aagaaaatca ggatgtgttc
    42961 gatacagttc agaatcaacc gagatcattt caggttcaaa ccataaacca aatttcaatc
    43021 ctttgctatg agcataatct gctatttcac gtaatccatt tgtcaacttg ccttcatact
    43081 cgaaccagtc tcctaatgaa gaattgtccg catctctttt tccaaaccag ccatcatcta
    43141 atacgaacat ttcgatgcct agctcctttg cttcatcaat gatcgccttg attttttcac
    43201 tattgaagtc aaagtaagtg gcttcccagt tattgaccag aatcggccgt tcagcaaatt
    43261 gatgacgacc tcttgcgatt ctttcacgta gcaactcatg gtggatctga ctcattttgt
    43321 tcaatccctg acttgagtaa gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat
    43381 tcaattccca cgaaaaacga tggctgttga tcccaccgac gactctcaat tgattgatct
    43441 gatctttttc aagttcgaaa gaatggttgc cggaataaac aaattgaagt ccgatcgctt
    43501 ctccagtatt ttctgtcgta tgatgatgaa ctaaagcaat aaagttattc atatggtgac
    43561 tgctcgttcc tcgacggctt tcaaatactt ttgttccaga taaaacagat tgccgtgaaa
    43621 tctgtctttc attgacatgt gctcctggca aagcaatgac ttcatcaaat cgcctagtat
    43681 gggcaaagtc tagctggaaa ctagccgctt tttctagttt gattgtttct ccggtttcat
    43741 ttcttatctt tacagatcgt gtgatcactg ctctatcagt aaatatggta tagaacaaat
    43801 cgaaataaat cgctgtttct ctatccttaa gtgaaatcac caacgtttgt gcctctgact
    43861 tgtccatcac ataagtttga ggcaagcctt cgatctctgg cttcccatct gtaatataat
    43921 ggctgtcata tctcagatcc agtaagtctg atccattctt gcgctgaatg atcgatgcgg
    43981 gcgttcgaaa gtccatgctg ccatgagaag agtattcttg cggtagtgta tctttcgaca
    44041 gagaccggtc aggatttaag ggaacatttc ctgaaaatcc acgatctctt cggggataat
    44101 ttttattgtc tttgtactgc ttgactggtt tgccaaaata aatatgcgcc aatacattgc
    44161 tttcttctat ctttatcaga taagaaagaa atgtatttcg taaatggaag actcggctct
    44221 tttcatcaaa taagatgaaa tttttatctg tttttctatg cgtaatatcc atcatttcac
    44281 tcccaacaaa acggaaaccg tttacttttt tttataattc ctgtatactg tttactaaac
    44341 aattttgtca ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag
    44401 agatgtcgca cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga
    44461 tcagacgatc catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt
    44521 ggattatgac atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat
    44581 ctctacgatc ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg
    44641 gatggaagaa gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga
    44701 taatcctaaa caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt
    44761 tacgaaagaa tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa
    44821 ccctgacatc cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac
    44881 cgtactggaa ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa
    44941 agtcgatatg gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc
    45001 ctatcagcat tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg
    45061 ctggacagaa aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct
    45121 tcctactgcc attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa
    45181 acaaaaaaat atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac
    45241 aatcgcaaac ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca
    45301 agcagctgtt cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt
    45361 gacttatcct gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg
    45421 aaagagaggt tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca
    45481 caacctctta cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt
    45541 cttttagcgt gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa
    45601 acaaggattg cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct
    45661 cctggaatga gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat
    45721 ggtcagccac actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca
    45781 acgaccacgc aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg
    45841 ctgttagctt caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg
    45901 gaagtagctg tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat
    45961 attgcaatgc gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta
    46021 gcaatttgtc gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa
    46081 cttgattagg gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt
    46141 tggcgtctga aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga
    46201 atcctccacc atgtacccaa aatagaacag gaacaggccc cttgctgttt tttggatagt
    46261 agatgtcata agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca
    46321 cgtttttttc tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca
    46381 tatggttgat caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa
    46441 acgcgatccc aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca
    46501 taaggctccc tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat
    46561 caagctctcc tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa
    46621 aagaaatttt ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc
    46681 acattgagga cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg
    46741 gtcaatcact tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga
    46801 caactaattt atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat
    46861 cgtattctaa ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg
    46921 tcatcccccc tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta
    46981 gtgttgaatc atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat
    47041 tgaaataacg aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca
    47101 caactcaaca gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa
    47161 gaaacaataa caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga
    47221 ttttagggcc ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc
    47281 ggcctccacc ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta
    47341 ttctccttta acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac
    47401 aggctgaact ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa
    47461 agttgccgga ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc
    47521 aataaaatga tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat
    47581 ccctttttct tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt
    47641 ttcgatttta ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc
    47701 catcccatac accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga
    47761 ataagcgtcg atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta
    47821 cagatactct gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt
    47881 ttttcctaat agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc
    47941 taaaatcaca tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa
    48001 ttcttcatga atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac
    48061 taaaacgacc atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg
    48121 tccttcattt ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac
    48181 tactttgatt gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag
    48241 atcatggcta ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg
    48301 gatctgtgct ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg
    48361 aacttctggc atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac
    48421 aagctgttgt tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt
    48481 gttttctaca ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc
    48541 aaacttattg cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc
    48601 gataaataat ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac
    48661 gatttcgact gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc
    48721 ctttaagcct atttttcaat cctactgaac tctactataa atcagctaat tacacaacta
    48781 aaaccggcac tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt
    48841 gtttcgtctg ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct
    48901 tttacgaatt ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca
    48961 tcaggaatct gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc
    49021 atcggtgctt ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca
    49081 tcttctgttt caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca
    49141 tgtcgaccgc cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc
    49201 ttcacaatga ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg
    49261 aatcctccaa cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc
    49321 ccttcatcat acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct
    49381 ttcgcaagat aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc
    49441 aattctgtca aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg
    49501 atgtccattt ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct
    49561 ctaggaattt ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt
    49621 ttaggacggt gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca
    49681 ccagctgcca ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct
    49741 gaatctggtt ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc
    49801 tgtaggatat ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt
    49861 acgcgtgctt ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta
    49921 ttcacgacac caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag
    49981 ccaaccgcac gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa
    50041 atttcattaa tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt
    50101 tcaactactt cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg
    50161 atttttgagc tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt
    50221 gtcaattttg tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc
    50281 atcaaccagt tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa
    50341 tcagacattt cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca
    50401 cggatatagc gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga
    50461 atccattcgt catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca
    50521 ctagaccctt ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt
    50581 gtttcttgtt ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa
    50641 gctaaacctt ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg
    50701 aactcttctt gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct
    50761 tccattttca catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa
    50821 gcgtatgctt cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg
    50881 ccttgtcggt tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca
    50941 tcttcttcta aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt
    51001 tcgatccatc cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga
    51061 ttatctgggt aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt
    51121 aatgccaaag ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg
    51181 cctggataac cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct
    51241 ggcgctggag agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg
    51301 actgtttcaa aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt
    51361 ctgttgcttg ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac
    51421 cgatgatctg caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg
    51481 gaagtccagc caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat
    51541 cgttaatgtt ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt
    51601 cataatctgc aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt
    51661 ttttgaagta agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc
    51721 gttttacttc ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt
    51781 tcacattttc agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg
    51841 cttctgatga ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga
    51901 cttcttccac aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt
    51961 ttacgccagg ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt
    52021 tgatgtcgcc tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt
    52081 ctttttcgtc ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg
    52141 tcattggtcc gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac
    52201 ggccataagt aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc
    52261 ccccagtatc actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac
    52321 caccagaaga gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat
    52381 aagaagtttc tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga
    52441 tcatatctgc ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt
    52501 aaaggatctt agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac
    52561 caattggaat accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg
    52621 cctgttccaa tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt
    52681 ctttgattcg ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa
    52741 gcatatgatg cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc
    52801 cccattatcg atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt
    52861 caataattca tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt
    52921 ttcaaccaca ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac
    52981 catatcgatg attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc
    53041 aagttttgct aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct
    53101 tctttctaaa cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa
    53161 attgtttatt gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt
    53221 agccaatgca tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt
    53281 ggtcagcttg ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct
    53341 accagtcact ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg
    53401 tgttagttta ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa
    53461 ttgggcattt cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat
    53521 caattcatgg acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc
    53581 gatcgtatca agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc
    53641 gaaatgttct gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa
    53701 aatcaaccgc tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc
    53761 tgatttttct tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc
    53821 agcaacatct gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc
    53881 atcgatattc atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg
    53941 gcatttagga ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc
    54001 acaaactgga caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa
    54061 cacgacttgc gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt
    54121 caagcggata tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt
    54181 cgttcctgct actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc
    54241 gactgtccat tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc
    54301 tgctgcccat ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt
    54361 cactttgatg acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc
    54421 ttcaatgtat gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg
    54481 gaatccaatt ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc
    54541 gaaatcagct actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc
    54601 taactgacgc aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc
    54661 tgcttctctg tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct
    54721 tacttcgatg ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt
    54781 ttcagtgatg ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc
    54841 atcttcataa cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac
    54901 tacacgtccc actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa
    54961 aacgtcgttc aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac
    55021 ttttccgcct actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc
    55081 gattgctact aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc
    55141 ttctacgtag tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt
    55201 agcagcttcg tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt
    55261 caattggcgc aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta
    55321 gttctagatc cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt
    55381 gtttgacttt atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg
    55441 gaacaaccgc tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg
    55501 tttttttagg cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta
    55561 caaaacgaga aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg
    55621 ttaaatacaa cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct
    55681 ccagttcgct ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa
    55741 tcaggaatac aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt
    55801 gggaagcatc ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga
    55861 ccgttagttt ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt
    55921 cgaattcttg tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta
    55981 atgtattttg gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca
    56041 attctgtcac tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg
    56101 cacctaattg ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt
    56161 caagcagtgg ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc
    56221 cgatgcctcg tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta
    56281 ccagcacatt caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc
    56341 ctccaaccat tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt
    56401 gtgcgttcgt ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct
    56461 cttcttgcat ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt
    56521 aagtgatctt gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat
    56581 tgttttcaat gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt
    56641 ctagcaaaat tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat
    56701 ctgcaccgcg ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa
    56761 atctacctgc caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat
    56821 ctacatgaat ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa
    56881 ataatcgaat cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct
    56941 ctttttggta agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt
    57001 ttgcaaaatc ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac
    57061 ttcgaggatc atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg
    57121 tcagctgttc agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat
    57181 ctcttcttaa aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc
    57241 cagatgcgag aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag
    57301 taatcgctag gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt
    57361 gtgtcagcac tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag
    57421 tgtgaagaac agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg
    57481 acattaacaa catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca
    57541 ttaaactagc tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt
    57601 tgatttcgcg ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca
    57661 agaaattaga taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga
    57721 caggaatcgt cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa
    57781 gtatctatga aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca
    57841 tccttgcagc atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg
    57901 ggattccaca agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt
    57961 tatggaaaaa gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag
    58021 aaggaccttc tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga
    58081 gtccatcttc agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag
    58141 cattcaatgc tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt
    58201 ctccacttgt ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt
    58261 actttttcgg cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat
    58321 attatggttc cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga
    58381 atgtcttgct ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg
    58441 ggttcgttcc tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg
    58501 gaggcaatca aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg
    58561 gtttgtttgt tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg
    58621 ggattttcct tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta
    58681 tggttgattt cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg
    58741 gttatttcac agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg
    58801 ttggctctct taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca
    58861 atgaccttct cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata
    58921 ctcagaccgc ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga
    58981 gttctttaga cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct
    59041 ctatacgcag aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag
    59101 atattttggt tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg
    59161 ctaaatcttc tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta
    59221 caacaactat tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg
    59281 ggaaaaccca gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca
    59341 ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga
    59401 ccgggtgact cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc
    59461 cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac
    59521 gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca
    59581 cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg
    59641 tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga
    59701 cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt
    59761 tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag
    59821 cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca
    59881 tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa
    59941 agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa
    60001 atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc
    60061 ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac
    60121 ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt
    60181 ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca
    60241 cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga
    60301 aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac
    60361 catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag
    60421 taccactaag ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac
    60481 gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga
    60541 ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac
    60601 cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt
    60661 ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc
    60721 tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt
    60781 ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt
    60841 ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca
    60901 aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc
    60961 cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt
    61021 taactgcact gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt
    61081 gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat
    61141 caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca
    61201 tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta
    61261 atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa
    61321 gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg
    61381 atctttcgta cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac
    61441 tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag
    61501 caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt
    61561 tttcccatac ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa
    61621 ttcgtgattg tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc
    61681 acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg
    61741 ataaaatcct tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg
    61801 tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc
    61861 taagccattg atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc
    61921 ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc
    61981 gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat
    62041 acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa
    62101 atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac
    62161 tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat
    62221 tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag
    62281 gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg
    62341 gctaaacgtt taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg
    62401 gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca
    62461 ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca
    62521 agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt
    62581 tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca
    62641 tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt
    62701 ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt cttagctccg
    62761 ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt
    62821 gattcagaag cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca
    62881 agcaagtcta ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata
    62941 caaataatag catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa
    63001 attataaatc agtcaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt
    63061 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
    63121 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
    63181 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
    63241 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
    63301 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
    63361 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
    63421 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
    63481 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
    63541 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
    63601 acagtcggat cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga
    63661 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg
    63721 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
    63781 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
    63841 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
    63901 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
    63961 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
    64021 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
    64081 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
    64141 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
    64201 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
    64261 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
    64321 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
    64381 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
    64441 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
    64501 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc
    64561 tgtttttttt cttcttcatt taagtctgtg agctggatag tcatcaataa tttatcttcg
    64621 ccaatcattt tctcacctct ttcaaatctt tttataattt aattataacc aatgataaag
    64681 aaaaactaaa actttatgtc ttagaaacca atttggacga ttacatgata gactaagtaa
    64741 aaagaaacaa agaggtgttt acaatggggt tgaaatttga acgtacagat aatttagata
    64801 aacttttcga atcatttgct gtcgatccgg aaaaaacagt tgcaccaaaa gaagagatcg
    64861 atcgatttct aaaaccacag ccacaaaatg aaaagaaaaa agacaagaaa caaaagtaag
    64921 ttaagctcaa aaaaagactt accagccaaa cttttatgaa agagtttgac tggtaagtct
    64981 tttttattct ttttatgatt caattgcttg ttcgattgct gcccacgctt cttcttttcc
    65041 ctgttttgta acggaagaaa agagaataaa cgtgtcattg cgatcaaaat ccaacgcttt
    65101 tttgatttcg ctctcatgct tgttccattt accacgaggg atcttatctg ccttagtggc
    65161 aacaacaatt acagggatat cataatactt caaaaattga tacatctgga tatcttcttg
    65221 agaaggctta tgacgcaaat ctactaaaga aacgaccgca cgtagctggt ctctagtcgt
    65281 taaatatgtc tcgatcatct ttccccattt tgctcgctca gttttggata cttttgcata
    65341 cccatatccc ggaacatcga caaaatgtaa tgcgttctcg atcaaataaa aattcaatgt
    65401 ctgggtcttt cctggcttac cggaagtacg ggctaaattc tttcgatcaa tcaatgtgtt
    65461 gataaaagaa gatttcccta catttgatcg tcctgccaga gctatttctg gcaattgtgt
    65521 atctggatat tgttttggtg ccactgcact tatgataatt tcagcttgat ggactttcat
    65581 ggtctctctc cttaacaagg tgatgaataa agcaagcggc tgtcgcaaaa gccaaaatga
    65641 cttttacggc agcctctttt catcaacccg cttttttctc tgaatcttta tagattactg
    65701 ttggctcacc agacaattca gctgcttctt ttgtgataat cactttttcg atgttttcat
    65761 ttgaagggat atcaaacatc acatccatca tgatttcttc aatgatggaa cgaagtcctc
    65821 gagcaccggt attgcgttcg attgcttttt tcgcaatggc tctcaacgct tcaggttcga
    65881 attcaagttc ggtattatct agagaaagaa gtttttgata ttgtttgact agcgcatttt
    65941 tcggctcagt caagatccgt accagatcat ctgttgtcaa tttctctaaa gctgccataa
    66001 caggtaatcg tccgataaac tcagggatca atccgaattt tagtaaatct tcaggaatga
    66061 tatgctgcat gacactttcg tcttcagaaa gtttttggtt ttgtgtacca aatccaatcg
    66121 tcttttcgcc catacgattc ttcacaatcg tttcgatgcc atcaaatgca ccacctacga
    66181 tgaacaatat gttcgtcgta tcaatctgaa taaattcttg atgtggatgt ttacgtccgc
    66241 cctgaggtgg cacacttgca acagttcctt ctagaatttt cagcaaggct tgttggacac
    66301 cttcgccgga aacgtcacga gtaatagata cattttcgct tttacgcgca attttatcga
    66361 tctcatcaat atagatgatc cctttttctg caagttccac attgtaatca gcagattgaa
    66421 gcaatttaag caaaatgttt tctacatctt cccccacata accagcttct gtcaaacttg
    66481 tcgcatctgc gattgcaaaa ggtacattca atgtttttgc taatgtttgt gctaaaaatg
    66541 ttttaccaga ccctgtaggt ccgatcaagc agatattgct tttttgcagt tcaacatcat
    66601 ctgtgttttc ttgtgcttgc tgattgacac gtttatagtg attgtatacg gcaacagcta
    66661 acgagcgttt tgcgcgatct tgtccaatca cgtagttatt aaatacttca agaatttcta
    66721 gtggtttagg gacttcagtg aattcgcgta cagcttcttc ataaaattct tcatcaatga
    66781 tttctttaca taggtcgata cattcattac agatgtagac ccctggacca gctacgatct
    66841 ttttcacttc ttcttgggtt ttcccgcaaa aagaacagcg tacggtctca ttgctacttg
    66901 gattgtcata catggtcttc accccttaac ttgcaaaaag tatgcaagaa actatcaaag
    66961 actttacagt cttttactat cataacatag tttagtaaaa agaaaaagtg tttaacaaag
    67021 gatcaactga cctatacctt tgcatttttt actcttaaca aaaaataagg aactgtgaca
    67081 aaatcgtgtc acagtccctt atttcgaata aacaattttc tagaagtagc ttcttcagaa
    67141 atgatttatt acttgatttc tattctttcg cttctgtcgc tgtacctgtg atcaattcga
    67201 ttgctttttt cattgtcaca tcatgttcca gcatatcttc tgtcaacacg cgtttgattt
    67261 gttcgattgg catgttgtat gtttctgata attcttggat ttctgcattg atttcttctt
    67321 ctgttgcttg aagattttca gcagctgcaa ttgcttcgat cacaagattt gttcttgtgc
    67381 gcatttctgc ttcaccttcg aattgtttat gcaagtcttc ttctgtagaa ccagttaatt
    67441 gatagtacat ttcaggagag ataccttgac gttgcatgtt gttcaagaat tcgtccattg
    67501 aacgatgtac ttcatcatga accatcacgt gtggcaattc aacgatttct gcgttttcaa
    67561 cagccatacg gatcgctgct tcatcttttg catcgtccgc tgctttttct tttgtttcta
    67621 ctaattcttt gcgatatttt tctttcaatt cgtctaatgt tgatacttcg tcatcaacat
    67681 cttttgcgaa ttcgtcatct aattcaggca cttcttttgc ttttacttca tggattgtca
    67741 ctttgaagac tgcttctttt ccagcaagat cttctgcttg ataatcttct gggaaagtta
    67801 catttacatc tagactttca cctgatttat gaccaactaa ttgatcttca aaacctggaa
    67861 tgaatgagcc tgaacctaat tcaagtgagt aattttcgcc ttttccgcca tcaaacgctt
    67921 cgccatctac aaagccttcg aagtcgatta ctactgtatc gccattttca gctgcagcat
    67981 cttctttgat aactaattca gcttgtgctt cttgttcacg tttgatacgt gcatccacgt
    68041 cagcatctgt tacttcacgg tcttgttttt caacaactag atctttatat tgtcctaatt
    68101 ttacttctgg tttcactgtt acttcagcag tgattaccca gtcttgacct ttttccatgc
    68161 tttcaacatc gatttttggt tgagaaacag gatcgatgcc agcttctttg acagcagctt
    68221 cataagcttc tggtaataca gcattcaatg catcttcata taaagcttct tcaccgtaca
    68281 tacggttgaa tacttgacga gaaactttcc ctttacggaa tccaggtacg ttcaagcttt
    68341 ttttcacttt attaaacgca gttgttaatc ctttttggat ttcgtcttgc gcaatcgtaa
    68401 attttaaaac accatcattc gtgccttttt tttcaaatgt tgcagtcatt taattccctc
    68461 cgagtttaga aattcatttt atacatcaaa aacgtttcat gcataccttt aaatagtaca
    68521 ctaacccttt caaattgtaa acatattgaa tggatagaat ccttgttttt cataacattt
    68581 ttatcttttt cactgataaa atctaatgga agaagatcat ttgctgcaaa attttttctc
    68641 ttttttttaa aaaatcttgt ttttcatctt ctgtaccttc atacatcgtt tttcctgtac
    68701 acgcctctac agtcagttga aaccagcgct tcgggtcttc gatgtatgta tcatagacag
    68761 gataaacaat tgcagattgc aacgtgaatt cttcttttaa cacggaacta agcagcgcat
    68821 cttcattccc gtaacgatct gaaaatacac ataattgctg gtaacttgcc tgctgctgcg
    68881 gctttggcag atcttttggt atgactttga ctaacttttc ttcaatcgtc aaatagaaaa
    68941 caggatggca ttcgttcaat tgcctaagtg tctccagtaa ttttgccctt gctaaaatat
    69001 ggacctctgg cgatacaata agtttactcg ctgtttggat aaactcatga taaggaagct
    69061 gttcgatctc atcaatagac gcaagctgac gtgcaggttc caacgaagga agttcctccg
    69121 atttattgtg gagtgccttg atcttctgcc tctccatttg tccggataat tcttctgcaa
    69181 attggatttt ttcaatgacc cgctgctcga aagcttcata aaaatctgta cttgctatca
    69241 attttctagc atataaaaaa tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa
    69301 actcctcatt ttgaagatac tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt
    69361 tctcgtccaa ctcgtaaaga caaaaaacaa gcaggctatt agcctcaaag ctttctttca
    69421 actcgtaagc tcgttgaaat aattcttttg cctgctgata ttgctggttt gctagtgctt
    69481 gttgtgccac tcgcattaaa ttctcatatt tttctggaaa atcaatcggc ttcacgcagt
    69541 gtcctcctct tgctcatatt cagctcatgt tcaatagtag caaaacaaaa aaagcatgac
    69601 aattaaaaat aactgaagca ccctctaaaa gctagatttt agatctgact tttagaaggt
    69661 gctttcaact gttactgctt tttattgctc atcagctgtt gacttcctgg ttgaacgctt
    69721 tgatcagatt gatcgtaaca acacttgtat tagtgaaaaa atcgcccaga tgttgctttt
    69781 tctgattaaa gactgctgga agatacccta acatcagcaa aggaaatttt agaatgaacc
    69841 ggcaagcacc ttctctaact aaaacggttg accagctaag ttcttcttca tcaaagctga
    69901 tgactcgaat accaaagatc attttcccaa tcgtttggcc atgattgaat ttcgtcaata
    69961 aaataaaata cgctagataa ataagtaagg ctgtgaatcc gtagagactc agataagaat
    70021 ctgaaaaatt aagccaacct agatttttgg caataccaag tgttaaacgc gtaatagctc
    70081 cgatacacaa aagatcgacc aagaaggcaa acattcggat aaaaaaacct gcatagaaat
    70141 acttaggaaa atcatttgct tttggcggtt catttcttgt gtccgtatac tttttccatt
    70201 tacgttgttt ctttttgatt tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta
    70261 ccttttggga aaagttggag tcaggtccta cagcaggctg ctgttgctta tctttttctt
    70321 gttccttatt ttcatttgaa agctctgaca tgcttactca cctccgtaat aatacattgc
    70381 ttttggtgat tccgctgtcc ctagattctc aagaacggat aagatctgac tagtctcaga
    70441 aggcttcaat ccctggaatt ctgctagttt acttcctaac catgtactag caaacccaga
    70501 tggcgtggaa gaatattcta ctagttccgc atcttcaagc tgttgttcct ttcgtatagc
    70561 ttttaaagca tcttcaggaa atcccaattc atcgactaaa cctacttctt ttgcttggac
    70621 accatcataa atccttccat cagcaatttt tttgacttct tcttcagatt tattccgtcc
    70681 ctcgctgacg attgctacaa aacgactata tgcgctgtcg atatatgctt gaagcaccgc
    70741 ttcttcttct tttgtctctg gtcgcgttcc tgaacccata tctttcaacg ctccgctttt
    70801 aactgttgta tcttccacac ccagtttttc tagaagtcct gaataattca atcctgacat
    70861 gatcacacca attgagccgg tcactgtttc ttctgttgca aagattttat ctgcttgagc
    70921 agaaatatag tagccaccgc tagctgccat gtttttcatg ctgacataca ttgggatatc
    70981 atgttcttta cgaatcgtat ccaatttctt agcgatttcc gcactttcgt agacaccgcc
    71041 accaggagaa tttacttcta aaaagatacc tttgactgtt ggatcttctt caatcgcctt
    71101 cagttgttcc ataaaatttt tatgattgta accttcagaa gcaaataaac ctgaagaccc
    71161 tcccgcagca atcgttccat ctactgataa ttttacgatt ttttgcgtac tgtctccctc
    71221 ttccaacaca tttggagcta gttcttcatc cccatataac caactgttca atccctcaat
    71281 ttgatcgttt tcctgaggct tagtcaaact agctgaaact aacgaaacca caaataatcc
    71341 tgcagcaatc agaactgctg cccaacgtct tttattcatt ctttctcctt tctaatacaa
    71401 gttttttatg acgatcacag tttctgactg ctcagaagtg tcgaaccgct ctgctttttc
    71461 ttcccgcgca tacccggaaa acgactcttt tgttaattca tcttctaccg taaactcacg
    71521 agcttgagca acttccaagg catcaggtgc gatttcttgg atgctatccc agccagtatc
    71581 taaaaatatt ttcgtatcat ctgatacacc agctgatgct tcgaatttgg acaattgttt
    71641 ttttaacatt cccagtgtca tgatctcaat ttttgaatcc atttttactc ctcctctatc
    71701 cgattacttt ctattgtacc gaactcctga aaaagatgat agataaaaac ccattatttt
    71761 ttgacataaa aaggacattt gacaaagaat ttgatatata ccaatttatt ttttattctt
    71821 tttttgttga caagaacatt cgcaaaaggt tatgatttta aatgtttcca gataaaaaac
    71881 aaaaaggagg atagttatac cttttaggat agcgccaggt ctgtgtaaac agatgacgag
    71941 gagagggttt atcgaaagat tcggcggatg gccctaggga ctgcactcta cagaaaatcc
    72001 acaaaaaatg tctgcaaaga taaaacaaaa ggattttcct gcagctggaa gcattcatac
    72061 gaattcatta tagaaagtag gatgtttttt atgtgcggaa ttgttggaat gattggatta
    72121 aaaaatgtaa caccaggatt gattgacggt ctagaaaaat tagaataccg tggttatgat
    72181 tcagctggta tctttgtctc agatggcaca actgattatc tggtcaaagc acaaggcaga
    72241 attcaaaatt tgaaaaacaa aataaatatt gatactacag gaaatatagg tattggtcat
    72301 acacgatggg caactcacgg gcaaccttct gaagaaaacg cccatccaca tacttcccaa
    72361 agtggacgct tcgtgctcgt tcacaacggc gtaatcgaaa actttgaaga acttaaaatt
    72421 gcttatttag caaatgacca ctttattggt gagaccgata ctgagattat cgctcatcta
    72481 attgaaactt tcgcgaagga tactacaacg caagaggcgt ttttaaaagc tttgcgggtc
    72541 atcaaaggtt cttacgcttt cgctttgatt gatcggacag cacctgatgt tatttacgtc
    72601 gctaaaaata aaagtccact attaatcgga ctcggcgatg gattcaatgt gattgccagc
    72661 gatgcaatgg ctatgcttgc tcatacgaag gaattcgttg agattgaaga tgaagagatg
    72721 gttaccgtca catcagaaaa agttattatt caaaattttg caggtgacat cgtagaacgt
    72781 tcttcattcg aagcacaagt agatgcttca gatatcgaga aaggtaccta tcctttttat
    72841 atgctgaaag aaatcgacga acagcctatt atcatgcgtc gtatcgctca aaaatacgta
    72901 acagaagaca atcgtgtctc tcttgatcaa aagctggtcg atacattgtc tgatagcgat
    72961 cgaatctata tcgttgcttg cggaactagt taccatgcgg ggttagcagg taaacagact
    73021 ttggaaaaat tgacacagat tcctgttgag gttcatttag ccagtgaatt tggctataac
    73081 acacctcttc tttcaaaaaa accattcttt attttcttga gtcaaagtgg cgaaacagca
    73141 gacagccgcc aagtgctggt aaaaatcaac cgtctcggct acccttcact tacgattaca
    73201 aatgttgctg gttctactct ttcgcgggaa gcatccttta cactgctatt gcatgctgga
    73261 cccgaaatcg cagtagcatc aacgaaagct tatactgcac aaatagctgt actaacttta
    73321 ttagccaaag ctatcggtga taaaaaagaa tttgaaactt ctcttgtatt cgatatcact
    73381 catgaattga gtttagttgc taatgcgatg gaatcggtca tcgcccaaaa agattattta
    73441 gaagagttgg cagaagtata tctcagcgat acaagaaatg ccttttacat tggacgtggt
    73501 gctgactaca acgtttctct tgaagctgct ttgaaattga aagaaatcag ttatatccag
    73561 gcagaaggtt ttgccgctgg cgaattgaaa catggaacga ttgctttgat cgaagaagga
    73621 acaccagtga tcggaatcat cagtgaagaa gtgactggtg cgcatactcg aggaaacctg
    73681 aaagaagtcg aaagccgtgg cgctaaaaca ctagttattg tctcagaggg attagaaaag
    73741 gaaagtgacc agctggtgct accaactgtc cacccttatc taactactct aaccacagtc
    73801 gtaccgactc aattacttgc atattacgca acgctattac ggggattcga cgttgacaaa
    73861 ccacgtaatt tagcgaaatc agtgacagta gaataataaa agaacaagga ccagtgtcag
    73921 acatctgaca ctggtccttg ttcttttatt ttttatgaaa atatctctta atccagtccg
    73981 atttcttctt tgactactgc cgcgatttta tcgacatagt aattaacttt ctcatcagta
    74041 ggcgcttctg ccatcactcg aagtaatggt tctgtaccgg aaggacgaac taggattctt
    74101 ccttctgatc ccatttcagc ttctgcctct tcaatcactt ttttgattgc cggaacatcc
    74161 atcgcaccgt ttttatcact tacgcggata ttcactaatt tttgcggata gattgttacc
    74221 tctgctgcaa gctctgataa tttcttacct gtctgtttca tgatattcag taactgaatt
    74281 cctgaaagca tcccatctcc agttgtgtta aaatctaaga aaatcatgtg cccagactgc
    74341 tcgccgccaa agttataatc gtttttgcgc atttcttcta caacgtaacg atcaccaact
    74401 tgtgtaacga catctttcaa accgattcct tccaccgctt tgtggaatcc taagttactc
    74461 atgacagtcg taacgatcgt atcttttttc aatcgatttt tttctgccaa gtatttagca
    74521 cagataaaca tgattttatc accgtcgaca atgtttccaa gttcatccac cgcaataata
    74581 cgatcgccat cgccatcgaa agctagtcca gcatctgctc ctttttcaac gaccatctca
    74641 gctaaacgtt ctggatgggt agagcctacg ccgtcattga tatttaaccc gtctggattt
    74701 gtgcccattg tataaaaatc tgtttcaaga tcggcaaaca aacgattaac acttgttgaa
    74761 gttgctccgt ttgctgcatc gacacaaatg gtcaaaccgg aaaggtcacc gttgatcgtt
    74821 tgtacaagga attgagaata tttcaataag ccttctggaa attcttctac agttcccaat
    74881 ccttcagctg atggacgagg caattcatcc acttcagcat ctaataatgc ttcaatttct
    74941 agctcttggt catctactag tttaaaacca tctgcaccga aaaatttgat tccattatct
    75001 tgtgctggat tatgagaagc agaaatcatg acccctgcgc ttgctttttg caaacgtgtc
    75061 agataagcaa cacctggtgt agagatcaca cctagctgga acacttcgat acccaccgat
    75121 aaaagtcctg aaatcagcgc ttcttctaac atttgacctg agattcttgt atcccgccct
    75181 actaacacgc gtgggcgttt tccttcttct tcatgctggc ttaggacata acctccgcac
    75241 cgtcccagct taaatgcaag ttctggcgta agttccttgt tggcttcacc gcgtacgccg
    75301 tctgttccaa aatatttacc catagttgta aaattactcc tttaacttaa ttttctgagg
    75361 aatttgttct gctcatttgt gttgattcgc ttgtttctga tgtttcttct gtacttgtgg
    75421 tcgagctgct tgtcccagat tccgaactgg aagaaatggt tgttgtcgat ggttccgttc
    75481 ctgaatcagc agatgaaata cttctactcg tacttgtatt ttcttgtgtc cgactgctcg
    75541 tcgaagcaga gctactgctt gacgtttgca acacgggtgt aatctccaca ctaactgatt
    75601 cgggcttgat acttaacccc tcttctactg gtatttcaat tttacgagtt gttcgatgat
    75661 tgatacctga gacattgatc ggtacagcaa tactgtcacc gatcgatgca agcaaatcgc
    75721 tagagccgct tacttctgct tccagttttg aaaaactgaa atcatagctt tctaccccgg
    75781 cagctggtgt tccttgctgt atcccataaa gtcgtaccga cttgcttggc ttgatcgaat
    75841 cagctctcac tgttacttgc actggatcag agacgatact taatacttcc cctgctgcat
    75901 tcagcgcctg tattgggacc tcactattaa tcccatcatc tgtgaccatc gaagcgtcca
    75961 catcagctac tactcgtttg atttctgcca atgttttgtc acctgtagtg atagagactt
    76021 ttttaggact aacggaagta tcatcaagtt catatccaga tggtgtgaac gttgaaggaa
    76081 tcaacgtttc cacatcgaaa tcttttatta cctttttctc aaccgttact gtaatagtcg
    76141 atggttcgat ctttgctgtg acagcactgc tcaaattttg aatttttagc ggaacttcat
    76201 gtgttccttc gcccagctta gtgatatctg ctgtgacgcg gaacatccgt gtatcttcgt
    76261 ttgcctctgc attcaactga actcggtttg cactggaaag tttgacagtc actgcatttt
    76321 catatccatg gatatagtag cgctttgaat cataagaaat cttgatcggc acccccgtaa
    76381 ctgtttgatt atacgcttca tttccagaaa gcgtatttgg aacggactgg ccatttgcat
    76441 tgaagtacag cgcaatactg aacagcaatg caataaaact gtaaagaatg cttgtccgtt
    76501 tctcttttga gatcatttgc gatcccctcc cttagtcata ccgtctagga aagattgaat
    76561 cacgtttttc ttgttttttg ttttctcttc agcaattagt tctctacgca agattgccaa
    76621 atattcttgc tgggataagc cagccatcag ttcattatct aacgtgatgc tgacgtcccc
    76681 ggtttcttca gagacaacga tggtaatcgc gtcactgact tcgcttatac cgacagctgc
    76741 ccggtgtcga gtcccgaatt ctttagggat caacatactt tctgatagag gaagataggc
    76801 gcttgctact gcgatctttc cttcttttac aataactgca ccgtcatgca gcggtgtatt
    76861 aggaataaag atattgatca gcagctcccc agtgatatct gcatctaaag caatccctgt
    76921 ctcaatatac tcatctaatc cagtatgacg ttcgatagtg atcaacgcac cgatttttcg
    76981 cttggacata tattggatgg ctttgtcaaa agacaagatc atcttctcat cttcctgctg
    77041 ttcgcttttt gattgtttga aaagcgaact gcgtccaaga tgttccagtc ctctccggat
    77101 ttccggctga aaaatcacga tagcagctat caccccgtac gtaatgactt ggttcatcag
    77161 ccaagacaag gtgtgcagac caatgatttc cgctacgatt cggataacaa taaaaatagc
    77221 tacacctttc agaagctgaa tcgcttttgt gcctcgcagc agttggatca gtttgtatac
    77281 aagataccag acaaccagaa tatcaagtat gttcaccaaa aaattcagag agaacaagtc
    77341 accggaaaac atttgctgcc aatagtccaa attaaaaagt tccccgattt gaaatgacat
    77401 aaaaagcctc tcttctcttt tgagatttcc tctctagtat atcatatgtt ttaattttag
    77461 aggctgatat gctcgtattt ttcttgagaa aaacgcataa ttttcaagaa aattgcaagt
    77521 atgcgtttta tttttctctg caataaatat ttttaaactt cagagaatta ttcattatat
    77581 tttttctgtt atttttcctt tttttctttt gtagcttgtt ccattttttt acgtcgttct
    77641 gctctggcag ctcttctagc agctcttctt tcttctgctt caaggtccgt cgttttttct
    77701 tttctagtct taacgggtac atcgatagat tcctctggtt caagtcgttt cttgattttt
    77761 tctaattctc ccgtcagtgc agcatagttg taaaaacgtg ttttagcgtc gtggacagtc
    77821 ttttcaaata aagcttttgc aagatttgga tttgcgttat ctagcgagga aaagcgaact
    77881 tgtttcctca tgaaatcagg catagcagag aaatcaggtt ttttgtagtc caaagtcatt
    77941 gggttcttgt ttttttcgac cattgtaggg ttatatcgat ataatgacca ataaccagaa
    78001 gaaacagctt cttttgcttc tttaagcgat tctttcattc cgccaagcaa gccatgtgtg
    78061 atacaaggcg tataggcaat aatcagagaa ggaccaggat acttttctgc ttcttcaaat
    78121 gccttgatcg tttgcatttg attggcacct gaagcaattt gcgccacata aacatttcca
    78181 tatgtcatcg ccatcattcc tagatctttc tttgaagcat actttccact agccgaaaat
    78241 ttagcgattg ccgaagctgg tgttgctttt gaggtttgac cgccagtatt ggaatagact
    78301 tcgttatcca aaacaaggat attcacgtct gctccgctgg caagtacatg atcgatcccg
    78361 ccaaaaccaa tgtcgtaagc ccatccgtct ccaccaatca tccattggct gttttttacg
    78421 aatagatcct ttctcttgta tatatcctcc agttcaggga aaattgatcg ttcgctttcg
    78481 atcgcagctt gcagttttgc tgctctttgc tgcgttcctt cactctcaaa catgtgtact
    78541 aaccagtctt ccatcaattc tcgtagttct tctgatactt ttggcaatac ttctttgatt
    78601 gaacttgcca aagcctcgcg acgagtctgt gccgctagaa gcataccata accaaattca
    78661 gcattatctt ccaataaaga atttgaccat gcaggccctt gaccacattc attggtcgta
    78721 taaggagaag caggagaagc accgccccaa atagaagaac agcctgtcgc attcgcgatc
    78781 atcattcgat caccgaacat ttgtgtcaat aattttacat aaggtgtttc gccacagcct
    78841 gagcaagcgc ccgagaattc aagaagcggt tgattgaatt gtgtagacaa gacagtattc
    78901 tttcctttga ctggatttgc tttttgtctt aatgtcattg caaaagccca attcacagct
    78961 tgttctttct gttcttcata aggcttcatc actaaagctt tttcttttgc gggacaagcc
    79021 tctacacaca gaccacaacc tgtacagtct tccactgaaa cttggatacg gtattttacg
    79081 ccgtctgcgc ctcgcatttc tctcacgata aatccttctg gcgcttcttc catttcctct
    79141 tcatctgcta aaaatggacg gatggcagca tgaggacaaa caaaggcaca ttcattacac
    79201 atcgtacagc gatcactaat ccactcaggt acttctaaag cgacgccccg cttttccata
    79261 gaagccgttc ctaaaggcat ttcgccagtt gtcattccat ttgctacaag tgtaccaaca
    79321 gagagttgat ttccttcctg tgcattcact ggttcaagta tttcttgtat ataaccagtg
    79381 ttccttttat tgattttttc aggcatctcc aacgttctcc aatggtctgg gatcgcgact
    79441 tgatgaagca gttcggtcgt ccgatcgatt gcttgtatgt ttttttctac gatagccatt
    79501 gatttatgtc catagctttt cagggcttct tcttttagca gcggaagcac ctcatcaaaa
    79561 ggaatgatgt cagctaattt aaaaaaagcc gtttccattg ccgtattgat ccggcgtccc
    79621 aaaccgactt cttgggccaa tctcatggca ttgatcgtat agaatctaat gttattttca
    79681 gccaagtaac gttttaattt taagggcaga tgcgtttcta attgttcgtc gctccaaaga
    79741 gtattcaata aaaaaatgcc acctggcttc aatcctttga ccaagtcata gctatgcagg
    79801 taagctggtg tgtgacaggc aacaaggtcc gcatgctcga caagatacgc tgaacgtatc
    79861 ggcgtatctc caaaacgaag atgagagaca gtcagaccgc cagatttttt cgaatcataa
    79921 taaaaatagc cttgcgcata tttatcggtg tgatctccaa taatcttgat agctgattta
    79981 tttgctccaa ctgttccgtc agatccaaat ccccaaaact tggcctggaa tgtttgcggt
    80041 tctgtcaaat ccagactttc ctttggttcc aaagattgat aagtcacatc atccacgatc
    80101 cctatggtaa accgtttttt gcgtatgctt gggtcttttt tcaattcatc aaaaactgct
    80161 gagatttggt cgggagtcac atctttagac cccaatccgt agcgaccacc aataacagcc
    80221 gggcgaagtt cagaatcata taacgcactt tgaacgtcta ataagagagg ttctccacct
    80281 gcccctggtt ctttacttcg atcaagaaca gcaattgttt ttactgtatg aggcaacttt
    80341 tgcacgaatg tttccactgg aaaaggacga taaagatgga tattcaaaaa acctgttttc
    80401 cgtccgtttg cattcaagta atcaatcgtt tgttcaatcg tctgtgcaac tgatcccata
    80461 gaaacaatga cttcttccgc atcgtctgct ccgtaataag tcaccaagtc ataagaagtt
    80521 cctcgtaatc ggttgatctc tttcatatat ttttgaacga tttcaggtaa tttatcataa
    80581 taggggttga tcgtttcccg ttgttgaaaa tggatgtctg gattctggtt cgtaccacta
    80641 acagtcggat gattgggatt catccctcgt ttacgaaatg aagctaattt ctcctgattg
    80701 atcagaggag ctaattcttc atattccaaa acttcgattt tttgaatctc gtgactcgta
    80761 cggaatccat caaagaaatt caaaaaaggg acttctcctt caatagaagc aagatgggca
    80821 acaggtgcta aatccatcac ttcttgtaca ctgctttcag ctaacatagc aaatcctgtt
    80881 tgtcttgcag ccatcacgtc tccgtgatct ccgaaaatat tcaacgcatt tgtcgtcact
    80941 gcccgactag caacgtggaa aacacttggc agtagttccc cagcaatctt gtacatattc
    81001 ggtatcatca aaagcagccc ttgtgaagcg gtatatgtag ttgtcagtgc accagttttc
    81061 aatgatccat ggaccacacc agctgctcct gcttcggatt gcatttctac tacttttact
    81121 ggttgaccga atacgttttc ttttcctgct gctgaccatt gatctactaa ttcagccatg
    81181 gtcgaactgg gtgttattgg atatattgca gcaatctcgg taaatgcata agaaatatat
    81241 gcggctgccg tattgccatc cattgttttc atttttcgca tgattcatca tccttgttca
    81301 tattctctct tattatctgt tccattataa cgtaaaacat gccatttttt tcttttattt
    81361 ataaaaatat tttacatgta tctatttgaa ttggtcatct aatttttaga caaacagtct
    81421 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    81481 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    81541 catcgaatgc tcctaaaaaa atggtcgaat tcatcaactt agcttgtccg tattgccgac
    81601 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    81661 gagtgatcaa attgttcgat aaagaaaaag aaagcttaca gcgagggaac gtgatgcacc
    81721 ggtatctcac aatcagcgat ggccaaaaag cgatcaaaga gatcaaacaa atattcgata
    81781 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    81841 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    81901 aagcacatat tcgatttgtt ccaactgtca tcttgggtaa agaaatcttt gatgaatcta
    81961 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtggga
    82021 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    82081 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    82141 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctaagcaaa
    82201 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    82261 ttttcataca aaatttctca atttccctta acgagtaata tttatgtacg gtgattctca
    82321 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    82381 ttttatgtat gctgttagtt gattagatgg tcatttgttg agctaggtcg tttattttaa
    82441 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    82501 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact attaactcct
    82561 ctcagctaat agttgctctc ttttttattc agattcattt ttgctgatca cagcctagta
    82621 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    82681 atggattagt agatttcgat tattcaaagc gattttctat tggtggatcg tgttcccaac
    82741 taataagacg gattccaaac ctatcgtaca gatcttctaa accacttata aattttgcac
    82801 ctatttcagg tgttttattg cgtggatatt ctattgttac gccattaaat ggataccaat
    82861 taggattagg ctctcttttt ttctgtacat ctcccataga atagttagca ctttctggga
    82921 cataatatat tcttcgacca tcctctaatt catctaataa gatatttcgt gatcgtctat
    82981 cacttgctaa atgtagacgc cacgtgcgat accatgccat gctacctgca tctgcagaaa
    83041 atccatcaaa taatgcaaca attgtatctt ctatcacaat cctttcccca tctacttcat
    83101 attctaatct aaaatcaaac tcccctcggg taatttctgg tcttggtggc ttaggaccca
    83161 atattattct acttaaaacc gcattcgttc ccagatgaaa cacagctata acaacaataa
    83221 tgattaatat tttgtttcta tttgataaat ttctcattat tcgcacatca cctctacatt
    83281 ctcacatatt agttcgtaat actcctttaa tatcaaaata tctactggtc gtaataatat
    83341 tcgctatgaa ttcctactac tcgtataaga aaggccaaat catcagcttt tacttgctga
    83401 tgatttggct tgtttctatt tatcgtaatt gttttctaat caccaaaatt gttaacaatc
    83461 ggttcagata tttcccagtt gattaaggta attccgtatt gtgtcaaaac ttcttgcgct
    83521 tctccgaaat taagtacagt tcttatgtat ccactttctc ctaatataaa gtctacctct
    83581 cttttaaaac cttcctcgag atttctaagg gctatatgtg gttttaatga cattgattgt
    83641 acattttccc ccatataata ttgcgcactt cctcgactaa aatagattcc tagatcgtcc
    83701 ccaaaaggta gatcttctct accactagct aatctacttc tccatcttcg ttctcttttc
    83761 atagaacctg cgctgaactc acacccgtta aactcagcta tgacagtatc ttcaattatg
    83821 ttaatctgtc catctagttc gtattcaatc ctaaatggaa attctccata ggtgatttcc
    83881 ggttcgggta ttttaggtga gattaatgca aagaaaggaa atattaagct tggcaacatc
    83941 aaaaaaactc ctcctatgat cacaattccc gatgtaatcc aaaatataat ttgtacaatt
    84001 ctcattgatc cattgtattc cattttattc cccctattac tattcatatc cgcctatcta
    84061 tatcgatctt ccaatgcaga gataattgct tccatacctt gattttttac tgagttacct
    84121 atgcgagata tcattctcca ctaaggtgca tcatattgtc tatctaacat gattaattct
    84181 ccaataggtg tatactcctc caaaaacaac atttaatgca tctccctcga caacataagc
    84241 taccatgttt gctgtatatg tgtttgagtc taattcataa gcagataaat ttactgtcgc
    84301 tggattaaat ataatcgcat tcgtatttat agctactgaa tttgctgctt cagcaccacc
    84361 ttttgaatgt ccgatcattg tgatttcaat agtaggattt acgtggacag ccctcaccgt
    84421 ttaccctgac cattatagaa atcgcttact tttttgaact acgcaacaaa ggatacaatg
    84481 ctcctaactt cactgtctcc atttttgtcc actaaggaat ataaaaaata ggcattaatt
    84541 catttaattc ggtcacaaca cgttaccttt taaatcatta tactgtatct catacattat
    84601 tatcaataaa ctattataaa aatgcaaaaa atgctttatc aatctatttt gtaattggtc
    84661 ataatatcat tgaaatcata ccctgtatcg atttttccta tgaatctcgt ctatttgtga
    84721 ttgacgaaaa agaggcaaga cagacactgg aatggtaatt ttacccacta ttttgctcca
    84781 actatttgta atcgtaccca cttttgaaat accgagatga tcattttaat tgatatgctg
    84841 attcccaagc aaaaccccaa gaaattaaat gctctatcgc atctagcttc ttgggttttg
    84901 cttatataaa ttccaacatt taaatttttt atgctgttcg gtctcagaaa tgccgttttt
    84961 gcttatatca caatatcaag atgtcaaatt attcgtgttg ccgatgactt tgattaattt
    85021 ttttcaaatt gtttattaag actaatgctg tctttttgca gataccaatg ttcgaacttt
    85081 ttaacattat catatgatcc tgcaagtttt aacttcttac cattagatgt tatcaaagtt
    85141 ataattaacc ttgccttttt cccattagga tcttttattt tatgaatttt ctgcaggtaa
    85201 tctctcctaa tggcagctat ttttacctca tcagtgctga taaaccattg atcatccaca
    85261 tacatactgc ctgttatgtt gtgtttttcc atttcttccg agaacgaaaa gtttaaggct
    85321 cgttcttact tccaaagttg aatgagtcca tggattggac ttagtgtgag aattgagaac
    85381 aacaataaga atccatatcc tcccgcaaac atccctaagg ctatactgtc tctccatcca
    85441 taagtaggat tagaagagaa aagagtcacc gtttgtaata atatgaaaag gaataatcca
    85501 gcacaaacac caaagataac aactccaaac attgaatttt tatacaatga ccatttattt
    85561 ataggctttt ctttcatttt acttccccct actgttctgt tcacacggaa tgaattcaaa
    85621 gttcataagc cctcctcttt gaacatatga atacgacttg ctaactatac tttaaaaaag
    85681 cttgttttaa gcggttatag gcgagttgta atctctattc atctcattag caaataactt
    85741 gccgaaaagt ctactgaaat taaagcagat agtggaagaa ttatttagcc aggatagaca
    85801 ctatttgtga aacacatgtc tttacttttg aaattctatg atgaaaagcc ttggaataca
    85861 gtagctctta attggtaata ctttttccat tataaataac ctcttttcat tttatattta
    85921 tcccctctaa aaataatatc taatctaata ttttaaactt atatttccct cgctgtgcta
    85981 atagagcatt actaaataaa tagaaactag aaaaaagcat tacgcctgcc agaataaacc
    86041 aattatttgt
//
LOCUS       DACDBG010000002        82313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDBG010000002 DACDBG010000000
VERSION     DACDBG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.002"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(216..2351)
                     /locus_tag="IVZ90_000078"
     CDS             complement(216..2351)
                     /locus_tag="IVZ90_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8261158.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2434..4089)
                     /locus_tag="IVZ90_000079"
     CDS             complement(2434..4089)
                     /locus_tag="IVZ90_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8261159.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4091..4405)
                     /locus_tag="IVZ90_000080"
     CDS             complement(4091..4405)
                     /locus_tag="IVZ90_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8261160.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4575..4835
                     /locus_tag="IVZ90_000081"
     CDS             4575..4835
                     /locus_tag="IVZ90_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8261161.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4849..5163
                     /locus_tag="IVZ90_000082"
     CDS             4849..5163
                     /locus_tag="IVZ90_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8261162.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5893..6447)
                     /locus_tag="IVZ90_000083"
     CDS             complement(5893..6447)
                     /locus_tag="IVZ90_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8261163.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6573..8501)
                     /locus_tag="IVZ90_000084"
     CDS             complement(6573..8501)
                     /locus_tag="IVZ90_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8261164.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8807..9523)
                     /locus_tag="IVZ90_000085"
     CDS             complement(8807..9523)
                     /locus_tag="IVZ90_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8261165.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9524..11347)
                     /gene="glpO"
                     /locus_tag="IVZ90_000086"
     CDS             complement(9524..11347)
                     /gene="glpO"
                     /locus_tag="IVZ90_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8261166.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11361..12857)
                     /gene="glpK"
                     /locus_tag="IVZ90_000087"
     CDS             complement(11361..12857)
                     /gene="glpK"
                     /locus_tag="IVZ90_000087"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8261167.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(12993..14453)
                     /locus_tag="IVZ90_000088"
     CDS             complement(12993..14453)
                     /locus_tag="IVZ90_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8261168.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14443..15738)
                     /locus_tag="IVZ90_000089"
     CDS             complement(14443..15738)
                     /locus_tag="IVZ90_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8261169.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16070..16747)
                     /locus_tag="IVZ90_000090"
     CDS             complement(16070..16747)
                     /locus_tag="IVZ90_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8261170.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16777..17868)
                     /locus_tag="IVZ90_000091"
     CDS             complement(16777..17868)
                     /locus_tag="IVZ90_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8261171.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(17969..18403)
                     /locus_tag="IVZ90_000092"
     CDS             complement(17969..18403)
                     /locus_tag="IVZ90_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8261172.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(18987..20444)
                     /gene="ascB"
                     /locus_tag="IVZ90_000093"
     CDS             complement(18987..20444)
                     /gene="ascB"
                     /locus_tag="IVZ90_000093"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8261173.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20441..22336)
                     /locus_tag="IVZ90_000094"
     CDS             complement(20441..22336)
                     /locus_tag="IVZ90_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261174.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22394..24169)
                     /locus_tag="IVZ90_000095"
     CDS             complement(22394..24169)
                     /locus_tag="IVZ90_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8261175.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYTVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24219..25715)
                     /locus_tag="IVZ90_000096"
     CDS             complement(24219..25715)
                     /locus_tag="IVZ90_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8261176.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25909..27531)
                     /locus_tag="IVZ90_000097"
     CDS             complement(25909..27531)
                     /locus_tag="IVZ90_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8261177.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(27980..29293)
                     /locus_tag="IVZ90_000098"
     CDS             complement(27980..29293)
                     /locus_tag="IVZ90_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8261178.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29538..29762)
                     /locus_tag="IVZ90_000099"
     CDS             complement(29538..29762)
                     /locus_tag="IVZ90_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261179.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29782..30150)
                     /locus_tag="IVZ90_000100"
     CDS             complement(29782..30150)
                     /locus_tag="IVZ90_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8261180.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30348..31058)
                     /locus_tag="IVZ90_000101"
     CDS             complement(30348..31058)
                     /locus_tag="IVZ90_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261181.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31076..31573)
                     /locus_tag="IVZ90_000102"
     CDS             complement(31076..31573)
                     /locus_tag="IVZ90_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261182.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31610..32062)
                     /locus_tag="IVZ90_000103"
     CDS             complement(31610..32062)
                     /locus_tag="IVZ90_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8261183.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32197..32802)
                     /locus_tag="IVZ90_000104"
     CDS             complement(32197..32802)
                     /locus_tag="IVZ90_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8261184.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33252..33872)
                     /locus_tag="IVZ90_000105"
     CDS             complement(33252..33872)
                     /locus_tag="IVZ90_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8261185.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33874..34743)
                     /locus_tag="IVZ90_000106"
     CDS             complement(33874..34743)
                     /locus_tag="IVZ90_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8261186.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34766..35173)
                     /locus_tag="IVZ90_000107"
     CDS             complement(34766..35173)
                     /locus_tag="IVZ90_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8261187.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35192..36481)
                     /locus_tag="IVZ90_000108"
     CDS             complement(35192..36481)
                     /locus_tag="IVZ90_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8261188.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36508..36810)
                     /locus_tag="IVZ90_000109"
     CDS             complement(36508..36810)
                     /locus_tag="IVZ90_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8261189.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36835..38928)
                     /locus_tag="IVZ90_000110"
     CDS             complement(36835..38928)
                     /locus_tag="IVZ90_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8261190.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39417..40181)
                     /locus_tag="IVZ90_000111"
     CDS             complement(39417..40181)
                     /locus_tag="IVZ90_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8261191.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40292..41161)
                     /gene="mdcB"
                     /locus_tag="IVZ90_000112"
     CDS             complement(40292..41161)
                     /gene="mdcB"
                     /locus_tag="IVZ90_000112"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8261192.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(41186..42262)
                     /locus_tag="IVZ90_000113"
     CDS             complement(41186..42262)
                     /locus_tag="IVZ90_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8261193.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(42286..42879)
                     /locus_tag="IVZ90_000114"
     CDS             complement(42286..42879)
                     /locus_tag="IVZ90_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8261194.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(42883..44559)
                     /gene="mdcD"
                     /locus_tag="IVZ90_000115"
     CDS             complement(42883..44559)
                     /gene="mdcD"
                     /locus_tag="IVZ90_000115"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8261195.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPLDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(44549..44848)
                     /locus_tag="IVZ90_000116"
     CDS             complement(44549..44848)
                     /locus_tag="IVZ90_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8261196.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(44950..46596)
                     /gene="mdcA"
                     /locus_tag="IVZ90_000117"
     CDS             complement(44950..46596)
                     /gene="mdcA"
                     /locus_tag="IVZ90_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8261197.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(46603..47514)
                     /locus_tag="IVZ90_000118"
     CDS             complement(46603..47514)
                     /locus_tag="IVZ90_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8261198.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            47634..48491
                     /locus_tag="IVZ90_000119"
     CDS             47634..48491
                     /locus_tag="IVZ90_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8261199.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(48678..49040)
                     /locus_tag="IVZ90_000120"
     CDS             complement(48678..49040)
                     /locus_tag="IVZ90_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261200.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            49386..50825
                     /locus_tag="IVZ90_000121"
     CDS             49386..50825
                     /locus_tag="IVZ90_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8261201.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(50832..53372)
                     /locus_tag="IVZ90_000122"
     CDS             complement(50832..53372)
                     /locus_tag="IVZ90_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8261202.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQQT
                     ILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQQL
                     IGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(53486..54817)
                     /locus_tag="IVZ90_000123"
     CDS             complement(53486..54817)
                     /locus_tag="IVZ90_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261203.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(54783..56261)
                     /locus_tag="IVZ90_000124"
     CDS             complement(54783..56261)
                     /locus_tag="IVZ90_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261204.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(56566..58401)
                     /gene="tdc"
                     /locus_tag="IVZ90_000125"
     CDS             complement(56566..58401)
                     /gene="tdc"
                     /locus_tag="IVZ90_000125"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8261205.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            58871..60298
                     /locus_tag="IVZ90_000126"
     CDS             58871..60298
                     /locus_tag="IVZ90_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8261206.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(60390..60755)
                     /locus_tag="IVZ90_000127"
     CDS             complement(60390..60755)
                     /locus_tag="IVZ90_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8261207.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(60752..61117)
                     /locus_tag="IVZ90_000128"
     CDS             complement(60752..61117)
                     /locus_tag="IVZ90_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8261208.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(61309..61557)
                     /locus_tag="IVZ90_000129"
     CDS             complement(61309..61557)
                     /locus_tag="IVZ90_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261209.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            61556..61684
                     /locus_tag="IVZ90_000130"
     CDS             61556..61684
                     /locus_tag="IVZ90_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8261210.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            61741..63435
                     /locus_tag="IVZ90_000131"
     CDS             61741..63435
                     /locus_tag="IVZ90_000131"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8261211.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            63502..63708
                     /locus_tag="IVZ90_000132"
     CDS             63502..63708
                     /locus_tag="IVZ90_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261212.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            64086..65219
                     /locus_tag="IVZ90_000133"
     CDS             64086..65219
                     /locus_tag="IVZ90_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8261213.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            65250..65570
                     /locus_tag="IVZ90_000134"
     CDS             65250..65570
                     /locus_tag="IVZ90_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261214.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(65599..66063)
                     /locus_tag="IVZ90_000135"
     CDS             complement(65599..66063)
                     /locus_tag="IVZ90_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261215.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(66195..67448)
                     /locus_tag="IVZ90_000136"
     CDS             complement(66195..67448)
                     /locus_tag="IVZ90_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8261216.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(67477..67794)
                     /locus_tag="IVZ90_000137"
     CDS             complement(67477..67794)
                     /locus_tag="IVZ90_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8261217.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(67820..68140)
                     /locus_tag="IVZ90_000138"
     CDS             complement(67820..68140)
                     /locus_tag="IVZ90_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8261218.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(68623..70038)
                     /locus_tag="IVZ90_000139"
     CDS             complement(68623..70038)
                     /locus_tag="IVZ90_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8261219.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(70055..71371)
                     /locus_tag="IVZ90_000140"
     CDS             complement(70055..71371)
                     /locus_tag="IVZ90_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8261220.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(71521..72219)
                     /locus_tag="IVZ90_000141"
     CDS             complement(71521..72219)
                     /locus_tag="IVZ90_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8261221.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(72508..73501)
                     /locus_tag="IVZ90_000142"
                     /pseudo
     CDS             complement(72508..73501)
                     /locus_tag="IVZ90_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(73582..74415)
                     /gene="msrA"
                     /locus_tag="IVZ90_000143"
     CDS             complement(73582..74415)
                     /gene="msrA"
                     /locus_tag="IVZ90_000143"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8261222.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(74649..75392)
                     /gene="truA"
                     /locus_tag="IVZ90_000144"
     CDS             complement(74649..75392)
                     /gene="truA"
                     /locus_tag="IVZ90_000144"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8261223.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(75583..76218)
                     /locus_tag="IVZ90_000145"
     CDS             complement(75583..76218)
                     /locus_tag="IVZ90_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8261224.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(76365..77606)
                     /gene="sstT"
                     /locus_tag="IVZ90_000146"
     CDS             complement(76365..77606)
                     /gene="sstT"
                     /locus_tag="IVZ90_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8261225.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(77927..78604)
                     /locus_tag="IVZ90_000147"
     CDS             complement(77927..78604)
                     /locus_tag="IVZ90_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8261226.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(78855..79397)
                     /locus_tag="IVZ90_000148"
     CDS             complement(78855..79397)
                     /locus_tag="IVZ90_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8261227.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(79402..79629)
                     /locus_tag="IVZ90_000149"
     CDS             complement(79402..79629)
                     /locus_tag="IVZ90_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8261228.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(79708..81588)
                     /locus_tag="IVZ90_000150"
     CDS             complement(79708..81588)
                     /locus_tag="IVZ90_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8261229.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            81874..82209
                     /locus_tag="IVZ90_000151"
     CDS             81874..82209
                     /locus_tag="IVZ90_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261230.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT        24178 a        17381 c        13860 g        26894 t
ORIGIN      
        1 accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt
       61 ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa
      121 tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt
      181 tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca
      241 atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct
      301 ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc
      361 aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc
      421 gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc
      481 aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt
      541 tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt
      601 ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg
      661 atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta
      721 aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc
      781 aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat
      841 gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca
      901 tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt
      961 tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga
     1021 tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa
     1081 atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc
     1141 acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt
     1201 ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt
     1261 tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc
     1321 ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc
     1381 aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca
     1441 ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga
     1501 tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt
     1561 gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca
     1621 atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt
     1681 tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc
     1741 ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct
     1801 acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct
     1861 ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta
     1921 ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca
     1981 tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct
     2041 acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt
     2101 tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc
     2161 gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt
     2221 tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt
     2281 tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt
     2341 gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct
     2401 tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc
     2461 aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga
     2521 ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata
     2581 ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt
     2641 ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt
     2701 tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc
     2761 aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg
     2821 tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt
     2881 tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat
     2941 ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata
     3001 gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc
     3061 tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt
     3121 tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc
     3181 ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt
     3241 tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc
     3301 tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc
     3361 aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag
     3421 tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac
     3481 tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag
     3541 aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc
     3601 catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa
     3661 tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc
     3721 tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc
     3781 gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa
     3841 gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc
     3901 tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa
     3961 gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc
     4021 cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac
     4081 gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta
     4141 cattcactac atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc
     4201 ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt
     4261 gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa
     4321 catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt
     4381 ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc
     4441 atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt
     4501 tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata
     4561 gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag
     4621 agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca
     4681 tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag
     4741 ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac
     4801 tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt
     4861 ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata
     4921 aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat
     4981 ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc
     5041 aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta
     5101 ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct
     5161 tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga
     5221 agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt
     5281 aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc
     5341 gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct
     5401 aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat
     5461 accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt
     5521 tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt
     5581 aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt
     5641 gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag
     5701 ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt
     5761 aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg tttggttggc
     5821 ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt
     5881 cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa
     5941 agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa
     6001 ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc
     6061 ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa
     6121 cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca
     6181 tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt
     6241 ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa
     6301 acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag
     6361 ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc
     6421 tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt
     6481 tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac
     6541 agccactgat attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt
     6601 ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct
     6661 cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg
     6721 taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg
     6781 tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact
     6841 aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa gagctggcgt
     6901 tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt
     6961 gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata
     7021 tccattttat gagcaatcca caaagcttgg ctttctcctg tttctccaac ccgcaactga
     7081 tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga
     7141 tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc
     7201 tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca
     7261 ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg
     7321 atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg
     7381 acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc
     7441 ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct
     7501 ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat
     7561 ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat
     7621 ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg
     7681 atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata
     7741 gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac
     7801 tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta
     7861 tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc
     7921 gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga
     7981 tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg
     8041 cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg
     8101 tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag
     8161 ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa
     8221 tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc
     8281 agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag
     8341 cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc
     8401 atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg
     8461 atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa
     8521 gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct
     8581 taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc
     8641 gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac
     8701 cagctacact cacaaaacat accccattct tctaggtctt atttattttc cacttaacta
     8761 tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg
     8821 aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat
     8881 atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac
     8941 caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg
     9001 ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa
     9061 aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg
     9121 gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg
     9181 ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta
     9241 aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca
     9301 tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag
     9361 ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt
     9421 tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg
     9481 tcccaataaa ctctcccatg atttgagtca tatcactgtt catttatttc tcccccttta
     9541 gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct
     9601 cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat
     9661 caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg
     9721 ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac
     9781 caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa
     9841 aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta
     9901 attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt
     9961 ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca
    10021 atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca
    10081 gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt
    10141 catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt
    10201 tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc
    10261 cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg
    10321 cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca
    10381 catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt
    10441 aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc
    10501 gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat
    10561 ctacaactaa atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat
    10621 tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga
    10681 tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat
    10741 catataaaaa gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct
    10801 ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg
    10861 cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca
    10921 gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg
    10981 ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa
    11041 gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa
    11101 ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa
    11161 gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt
    11221 tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga
    11281 tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg
    11341 aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa
    11401 ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct
    11461 tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa
    11521 aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag
    11581 atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat
    11641 acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa
    11701 gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca
    11761 aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga
    11821 acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct
    11881 ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata
    11941 cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa
    12001 ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca
    12061 taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg
    12121 tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa
    12181 ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa
    12241 tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt ccgactggca
    12301 ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg
    12361 atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca
    12421 agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg
    12481 tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg
    12541 gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt
    12601 gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg
    12661 atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc
    12721 acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt
    12781 cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata
    12841 tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc
    12901 ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt
    12961 tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct
    13021 attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca
    13081 cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca
    13141 taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa
    13201 gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata
    13261 taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta
    13321 tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac
    13381 tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag
    13441 acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct
    13501 actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc
    13561 cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga
    13621 taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt
    13681 tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt
    13741 gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta
    13801 cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct
    13861 gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt
    13921 tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca
    13981 taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat
    14041 ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg
    14101 atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg
    14161 agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca
    14221 tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc
    14281 atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt
    14341 tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc
    14401 aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt
    14461 attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc
    14521 atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag
    14581 aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc
    14641 aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta
    14701 tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc
    14761 taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg
    14821 tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga
    14881 aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata
    14941 aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa
    15001 tgttaattgt tcttcccaca tctgattgtc ggggcgagaa tgattagcca gcaaaacaag
    15061 atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga
    15121 cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg
    15181 ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg
    15241 gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc
    15301 aataattgca attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat
    15361 ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga
    15421 accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt
    15481 tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt
    15541 cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat
    15601 caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc
    15661 acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc
    15721 gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct
    15781 caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc
    15841 agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg
    15901 acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga
    15961 aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg
    16021 agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta
    16081 atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa
    16141 gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt
    16201 tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct
    16261 cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat
    16321 ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc
    16381 gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc
    16441 cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta
    16501 aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt
    16561 catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa
    16621 aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc
    16681 acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata
    16741 ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa
    16801 attatataat gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac
    16861 aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg
    16921 ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg
    16981 gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag
    17041 aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac
    17101 atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa
    17161 acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt
    17221 tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata
    17281 acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa
    17341 ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat
    17401 cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat
    17461 tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg
    17521 ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc
    17581 ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc
    17641 atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc
    17701 agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt
    17761 gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt
    17821 caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc
    17881 cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg
    17941 tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat
    18001 tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat
    18061 gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat
    18121 aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt
    18181 caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg
    18241 gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg
    18301 tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa
    18361 agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg
    18421 cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc
    18481 ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga
    18541 caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt
    18601 atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
    18661 gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca
    18721 agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg
    18781 ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc
    18841 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt
    18901 gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt
    18961 ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt
    19021 ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct
    19081 tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata
    19141 tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc
    19201 atctgctcaa tatgctgttt caaatagtca atgcggtaat cgtcttgaac ggttccatct
    19261 tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac
    19321 ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat
    19381 tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct
    19441 tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa
    19501 tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct
    19561 ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta
    19621 aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa
    19681 tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca
    19741 tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc
    19801 gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt
    19861 tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg
    19921 aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca
    19981 tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc
    20041 ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt
    20101 gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta
    20161 aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg
    20221 tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc
    20281 gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac
    20341 acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc
    20401 gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac
    20461 ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac
    20521 tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca
    20581 aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt
    20641 gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc
    20701 cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt
    20761 ttacaggact gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc
    20821 cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag
    20881 tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa
    20941 aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc
    21001 cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg
    21061 ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg
    21121 gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg
    21181 tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc
    21241 caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac
    21301 gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa
    21361 tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat
    21421 aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa
    21481 ctaataaagt aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa
    21541 tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta
    21601 aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc
    21661 gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa
    21721 taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata
    21781 taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt
    21841 cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc
    21901 ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta
    21961 aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt
    22021 gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc
    22081 ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct
    22141 tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat
    22201 tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta
    22261 cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag
    22321 aaaacggttt ctccattcaa ttagtacccc ttataatagc gaacgaaact gtacaatagt
    22381 gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt
    22441 ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa
    22501 gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc
    22561 acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt
    22621 gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat
    22681 caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat
    22741 aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact
    22801 caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt
    22861 gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt
    22921 gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta
    22981 tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc
    23041 tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa
    23101 tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg
    23161 catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca
    23221 ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct
    23281 tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat
    23341 tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt
    23401 ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt
    23461 cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg
    23521 aaacagtata attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga
    23581 tattggaaaa aattgtactg atttgttgag gcaaccttgc gtgttggatc accgtttctg
    23641 ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt
    23701 ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt
    23761 ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac
    23821 tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc
    23881 tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa
    23941 ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga
    24001 tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag
    24061 ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt
    24121 gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac
    24181 ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat
    24241 tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact atcttttttc
    24301 aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc
    24361 ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca
    24421 atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac
    24481 tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg
    24541 ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa
    24601 cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag
    24661 ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag
    24721 acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt
    24781 tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa
    24841 gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc
    24901 agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag
    24961 ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg
    25021 gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca
    25081 atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata
    25141 cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt
    25201 tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc
    25261 ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg
    25321 atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt
    25381 ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct
    25441 tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc
    25501 gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct
    25561 tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta
    25621 ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc
    25681 cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa
    25741 tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga
    25801 agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata acaaatacat
    25861 aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc
    25921 caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg
    25981 ataatttgac acaataaccc ttaaagcttc acgtggaaca gtgaacgtct gcttttcact
    26041 ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac
    26101 atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct
    26161 acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc
    26221 ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa
    26281 cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc
    26341 attgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat
    26401 attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc
    26461 taactcttct ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa
    26521 catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg
    26581 aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca
    26641 tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt
    26701 gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct
    26761 tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt
    26821 catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt
    26881 aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat
    26941 acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg
    27001 aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata
    27061 ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt
    27121 aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt
    27181 atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc
    27241 catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat
    27301 cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt
    27361 cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc
    27421 caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact
    27481 tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct
    27541 cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa
    27601 attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa
    27661 acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg
    27721 ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa
    27781 aaaacggcaa aatatgagga actatttttt cgtttttttc tctaatactc aggccagaac
    27841 actgatttca caacctcttt ataaacggtg ttcactcagc tgtccctcgg tattagctca
    27901 aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa
    27961 cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa
    28021 agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa
    28081 ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc
    28141 cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca
    28201 aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa
    28261 caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag
    28321 ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa
    28381 caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt
    28441 tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta
    28501 aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt
    28561 gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg
    28621 gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg
    28681 ttgtagctgc tatcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag
    28741 ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta
    28801 ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa
    28861 atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca
    28921 tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg
    28981 ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat
    29041 tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac
    29101 agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag
    29161 cacctaatac agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc
    29221 ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta
    29281 ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat
    29341 ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca
    29401 aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa
    29461 agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt
    29521 cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct
    29581 ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg
    29641 accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc
    29701 ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc
    29761 actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt
    29821 gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg
    29881 ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg
    29941 tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc
    30001 taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc
    30061 agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac
    30121 ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct
    30181 ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac
    30241 tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac
    30301 aaaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct
    30361 tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt
    30421 ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat
    30481 tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca ttgttctttg ggcttcacgc
    30541 cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt
    30601 gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct
    30661 aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac
    30721 aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga
    30781 tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg
    30841 gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg
    30901 taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct
    30961 tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat
    31021 acttcccaga aattggtatc cactattact tttttcatct gttcacacct cttgattaga
    31081 ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt
    31141 cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc
    31201 aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta
    31261 gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt
    31321 ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac
    31381 ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt
    31441 ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat
    31501 tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat
    31561 ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc
    31621 ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta
    31681 aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg
    31741 agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga
    31801 gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta
    31861 atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt
    31921 cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta
    31981 aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga
    32041 tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag actagtttac
    32101 ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact
    32161 agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt
    32221 gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc
    32281 ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc
    32341 accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt
    32401 ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc
    32461 ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc
    32521 aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt
    32581 gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc
    32641 ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg
    32701 gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga
    32761 aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt
    32821 agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata
    32881 taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac
    32941 ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac
    33001 caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca
    33061 gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca
    33121 caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg
    33181 aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag
    33241 ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg
    33301 taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg
    33361 tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct
    33421 ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga
    33481 cgatctgatt cacgatcacg tttgtaccga cgttgacata acacttcaaa gtctgctgtt
    33541 aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt
    33601 tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa
    33661 tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc
    33721 aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca
    33781 tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga
    33841 ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt taattggact
    33901 ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc
    33961 tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc
    34021 tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt
    34081 gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac
    34141 atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa
    34201 ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa
    34261 tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc
    34321 ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata
    34381 ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata
    34441 catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg
    34501 gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac
    34561 taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga
    34621 acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa
    34681 agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata
    34741 catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa
    34801 tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac
    34861 gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga
    34921 tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca
    34981 attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg
    35041 tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg
    35101 gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa
    35161 aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga
    35221 tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta gcattgatga
    35281 atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat
    35341 gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg
    35401 ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca
    35461 tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga
    35521 cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa
    35581 ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac
    35641 cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga
    35701 tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga
    35761 tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga
    35821 aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa
    35881 gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat
    35941 catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga
    36001 aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat
    36061 agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt
    36121 acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca
    36181 gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag
    36241 aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac
    36301 ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg
    36361 tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca
    36421 atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca
    36481 tgtttttacc ctcttttctt tttgtattta gataactccc ttatcttgca gaactggggt
    36541 gattttttct tcaatttcat tttttgaaac aatatttttc aaatagatca tcgccgtttt
    36601 aggatcaact gcgaactttt cgaactgcga acgaaaattt tctgctgtga tgatcacatc
    36661 tggtttcatg gaaactgcgg aagaaatatc acaatggtcc agtttggctt ctactcctaa
    36721 tttgttcaaa acatcctcag cagacatctg tgctgcaaaa ctacttccta aacctgcacc
    36781 gcatacgaat aaaatgttca atttgttcat ctttgttctc ccccgtatca tttattattt
    36841 ctcaactact gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt caatcaatcg
    36901 atccaacttc tcttcgttgt tgatcaattc aattagttct ttcatgatgt tcagatgcga
    36961 gtaggagtct actgctgcca aacaaaaaat aatctttact ggatccattt caggattacc
    37021 gaaatcgatt ggttgctcaa tcgtagctac gctgactcct aatttattga ccccatcttc
    37081 tggtcgggca tgggctagtg ccatgtgttt gccaatcacg atatacggtc cgtaatcatt
    37141 cactgcacga atcatcgctt cgacatagcg ctcttcgaca atgttttctt taaccaatgg
    37201 tctagcagct agttcaatcg attcctccca agtagtggct ttttctttga tcatgatatt
    37261 gctgtccgtc aaaatatctt ttaacatagg ctggatctcc cttttgttga tttctaaatt
    37321 gtttgtttcg aataattttt ctaacttgcc atagatgatt cgatctactc ggccaccact
    37381 ttctttcatg acatctatta tttgagtgaa taactccgtc gattcatgac tgttaggctc
    37441 taaccgctgt agttcttcat ttcttttcaa aaagtcttct ataaccaaga tattcgtctc
    37501 agttaaaatc ggatcgacaa ctaaaagcgg cttatttcga taattcaatt gaaaggtgct
    37561 gaaaaccaga ttgacatcca atttatctac taattcgatt tcttttgaac ttaaaactgc
    37621 agtaatctcg atctgaggaa acttttcttt cagattctct gctaacagat ttccagtagc
    37681 catcccatgg ttacagacaa cgactgattt ataaacataa ttcaagtctc gcttgattac
    37741 actggcgatc gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc
    37801 accaattact tcttcgatcg taggaacaaa atgagtgact gcgcggtaga ttgggaaata
    37861 atttgttcgg atgttttctt tcaatggatt catgacttgg atatgattct tgattcttac
    37921 aatcagtgca gccatgtgct tatagagatt ttcgaataaa gcttcttcct ttagatggaa
    37981 aggtacgtgt gtctcgttct ctacaaattg gatcaattgg atcgttagta attgagcttg
    38041 tacccactcg attgaattgg agatatctct agcattgaac gtctcaatag tgaaacgaag
    38101 ataatttctt tctacttgcg ggacattgtc caattgaaat tcctcgatca tgtggtcaat
    38161 aaatgcagaa aagctactgt cttttatttc tgtatttttc caattaatgg cagagataaa
    38221 ttcatgtttc ctgattcgct ggatccagat caatgtaaaa agcaggattt gttttttata
    38281 aatatcatct tcttgtttca acaacttatt ggaatagatc tcctgtattt ttttgatttc
    38341 caccaaagga aaataatgga gaaagatttt ttgagccggt gttaattgtg catcttgatt
    38401 cataaagaag tcggtcattc ccagattctg attgataaaa tcatagatca tcgtacggat
    38461 agatcgttct gaacctttat aaaccagtcc ttgctttcca taactgacga tctctatccc
    38521 ataactgacc agtgttgctc gtaaccgtct catatcttca tctaatgtac ttttagagat
    38581 ttgaaattct tcttcttttt tgtttagatg gatcgggatt cggctgtaag caacggctaa
    38641 aactagatca aaaatacgtt cgcgccgatt caaaaagccg tctttcacat cttttatttc
    38701 agtttctaat cgagcttgct tttccttttc cagcttcaac aaaaaaactt tgttgcgaat
    38761 tgtacggatc tccggaagct ttttactgct caaccagata ttcgcttcaa gaacttcctg
    38821 acggatcgtc cgctgactga cattgaactc acttgctaga tgttttgctt ttactggata
    38881 ttgggctgtc atgattctgt gaagtatttc aattgttctt gttttcatag aaatgatctc
    38941 cttctgattt caagtatatg gttccgctta cataataaaa agtagatatt tctgccactt
    39001 gaagtggaaa gaaagtaagc aaagcaaaca aaaaataagc catatgcttt ttcatctcct
    39061 caacgaaaaa gacatacgcc tattattaca aaaattttgc ccatctatac aaaaaagagg
    39121 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacaact aaactaaaat agtgcttcct
    39181 atttttcgtt tggttgttct tattatcgca gatgcaagct tttaaacacc atttagtgga
    39241 gaggttgtga cagagatgtt cagccttgag cgataagaag cgattttgaa aaatcgcttc
    39301 ccatatttta caaaatcgtg ccttattgcc gataggctat ctctggcaca ctgtttattc
    39361 agaaaaagga gctgcgacaa ggcttttgtc acaactccgt tcataaatat ttcctgtcaa
    39421 tccagcataa tcggctgaat aaaatcggct tgccatcttt tttctttctc agaaaaatca
    39481 aaataccata tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat caatcgccca
    39541 tcttttcgat cgactgaata atcacagcat ctgaaatcaa ttcggatggc aaaactattt
    39601 tcgtggatct gtcgataatt tcctacttcc tgaatagtta tttttttagt atgatttctt
    39661 acaccgtctg ctttgttttt ttgcagaact ttctgatgtt cggcaaacag cttgtctgta
    39721 taacagcttc tggctttttc aggagtttct tcttcccaag cggattggat cttcatgaat
    39781 atcttttgga tttccagaaa ctgtgctttc ttttctttag ccgttcctga catgcgggac
    39841 aatatctccg ctttcagcct tttcttttgt cttttattct tgacgataga aaaagcagaa
    39901 acccctccta ctgacaaaac agctaatgca agaaatcccg tttgctcata actagaagga
    39961 cctgtacctg gaccataata cccataagaa tctctgccgt aagaacgact gccaaaataa
    40021 ctgcccccat atgaacgacc accaaatgaa cctctgcccg aacttcgacc gctagatgta
    40081 cctccaccat gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa caaacatagg
    40141 cccactaaca aaatgattgc taaataattg atttttttca ttgtcttcct cgttatcttt
    40201 tattacaaaa aactattttt atgatagcct tgctaaaagg aagagtcaat aaccagatgc
    40261 ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacaaatgt
    40321 atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat
    40381 ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg
    40441 gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg
    40501 ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca
    40561 acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct gcttggattg
    40621 caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt
    40681 tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag
    40741 cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga
    40801 ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat
    40861 tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc
    40921 tttctcttaa ctcctgatca atagcatgat gccatgctat ttgagccatt tcttcaaaaa
    40981 aaggagttag cgtcttagca gacttaagaa ataaagcata gtccatatcg gaatgtgcac
    41041 ccgcatccag tgcatccact aatccaggtt ttggacttaa tgttacttct gcgatcaatg
    41101 cttcctctac caacgcagcg agttgctgag tcactgcttt ttttcgttcg tcatttatca
    41161 tgacactcac tccttcttcc tactattact tgctgaaacc ttttttgata gctacccaaa
    41221 tgatatgaag taaccagctt aaagcaaatg cacaacttgc cgtcattaga taagctacaa
    41281 gaatcatgac tcctgaaggt aactgcagcc aactatcttt aaacaataat aacagtactt
    41341 ggaaaacaaa tacattggct aaatacgtac gataagcata aacagacaac catttgaaca
    41401 atggcaattt tggcgaatcg tttacaatca ttttggaagc gcctatgaag acagcaaaga
    41461 tgatgactaa actgtataaa ctttggatcg tattcaagta aggtgcattc gcaaagctca
    41521 agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa cgcgataact aaggcaacag
    41581 gtaagaaaag ataacgtact ctgtccaaat attgatagac tttttcgtga taaacaaatg
    41641 cggctacccc ataaatcatg aaactagcaa caaaccgatc taataagtac cagctgtcat
    41701 gataaggccc agtcaatact tgtgtttgat agaacagata ccatgcgaca tataaaatcg
    41761 ttgaaccaat caatactggc catacatatt ttttattagc aaatactttg taaccgagcc
    41821 aaacaaaaaa cggcatgaac aattggatct gcagcatcat aacggtatac cataaatgag
    41881 gcgctccatc acctgtgaca tatttcagta aaaagcttgc agtatccgta tacggagttt
    41941 gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga tacggaacga
    42001 ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctga cttgttttta
    42061 ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa
    42121 aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca aagctcaata
    42181 tcgtttgcat cataacagcc gttacagctg atactttctg gaagtcactt acgttgattt
    42241 tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc aaagttcttt
    42301 ggttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct ctctcaaata
    42361 ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa
    42421 agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg cgtacagcag
    42481 caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt caaatcctaa
    42541 gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat gaaattgctg
    42601 ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga tgatgtccca
    42661 cggatgaatg acttgtttga cggcatgttt tgagatttct gttgaccatc tctgattgcg
    42721 ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac
    42781 tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc cttctatttc
    42841 atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat cccattcttc
    42901 gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt
    42961 tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt
    43021 gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg tgatatcttc
    43081 taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag cgatagctgg
    43141 aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac gtgcagcact
    43201 ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca agcggcttga
    43261 ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca atccaataac
    43321 tggatgtcca gcttgacgga gttttgcata agcagcagca ctattagcca aagccacatg
    43381 gataccgatc aattcttcgt tatagccgta tgcttggctt ggtacatcga tcacaacgat
    43441 gattggccgt ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc
    43501 catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca tcgggttttt
    43561 aggatcaggt acgattgatg tcacgatggc ttcttctatg aatttacttg atttaccata
    43621 atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg caaaccaatc
    43681 gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta agtcaattga
    43741 ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg gttttgaaag
    43801 atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt
    43861 tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat cagtcactaa
    43921 ttcatcaatg atttttgatt ctacccgttg acgagatcct aatgtattcc aaatcaagtc
    43981 tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt
    44041 caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg aagtaatcga
    44101 cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta catattgttt
    44161 tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt ttgcttcttg
    44221 caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc cttttttatt
    44281 ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt caccgatacc
    44341 gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat tgatcgtgcc
    44401 acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg gtaaacttgg
    44461 cgcgatgatt tgatcgagtg gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac
    44521 tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc tccattgctt
    44581 gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc acgatatctg
    44641 ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact tcgccgaagc
    44701 catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga aacatcaaga
    44761 tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc ggctgtttcg
    44821 ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa acagagcata
    44881 cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa aaggtttttc
    44941 tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc catctgacca
    45001 ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt cactacgttt
    45061 gatgcctaga tcttctggta atttcacgat gccggctgca cgcaagctat ctagtgattc
    45121 tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg
    45181 ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca cgatatgtgt
    45241 agtatcttcg ctataaatca tcactggtgc attggctaag cctgcttgtt tttgaacagc
    45301 tagtgcatct aattcttcga caaagactgg ttttttattt gaaccatatg tttcaaccat
    45361 ttgaacaact aatttttggc ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca
    45421 tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg caccgccaaa
    45481 accagataaa cgtccttttg ttacggtaga agagtttcct tcgtaatcta attgcaaggt
    45541 tgaaccgata aacatatcga tggcatattg tcctgcagct tgtgataatg ttcggttact
    45601 gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa tatatttttc
    45661 cataccaact tcgccaccaa aactatggat gctttcaacc caacctgatt cgatggctgg
    45721 aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt ttaaccctag
    45781 tgattcacca taagttggca gtaacaattc gatagctgct gtattgaatc caataccgtg
    45841 attcaaagat tgtacgccgt gttctgcgta gattcctttg attgccatca ttgccattag
    45901 aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta attgataagg
    45961 tttatctgct ttcacgacaa cgtctaccca gtcaccaggg atatctacac gtggtaaaga
    46021 atcgacaact tcgttgactt gaacgatcac gataccatct ttgaaagctg cagcttcgat
    46081 gattgttggt gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt
    46141 atctgcagct actaaaacaa catttggaat caaatcgatg aataagcgtc cataaagttc
    46201 caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt gggcgacacg
    46261 caagctttgt gcaccagcat aagagaaatc gatcttgctt gcgattcctt tttcaaaaat
    46321 attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat tcactttttc
    46381 tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat tgtcgccttc
    46441 taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga ttttttcagt
    46501 ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg cttttttttc
    46561 gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc atttttgata
    46621 tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga tccctttatc
    46681 tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa attcaatcgt
    46741 cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca cagggaaagc
    46801 taggttttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt
    46861 attttttata tacaaacatc tagcatcaga tagatggaca gactgcatat acggcttgaa
    46921 tttcttgacc gtatcattca tcaaaagaca gtgagatggc acagggactt tcagcagctt
    46981 cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac cttcgatact
    47041 cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg gatggtcagg
    47101 atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc gtcacaccta ctactacgcc
    47161 cattgcataa ccgcttggat aagcttcttt cattaagact gcgcggtgat agactagttc
    47221 cgctgcattc tcaaatctca aagatcgaca agcaacagct gcacaaaaag caccaatcga
    47281 atgtcctgct gcaatatcag gctgaatacc gtttttttcc atttcatcta aataaaatac
    47341 tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt cttctttatc
    47401 aactaaccga taccccgtca cattttctaa acgttccatg tacatttcag ggatatccgc
    47461 taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac tcatgagcct
    47521 cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct ataataaaaa
    47581 atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata attatgaaat
    47641 tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca
    47701 aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg gaaaacgaat
    47761 taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt tatgcagaag
    47821 aattttatcc atatgcagtc aagacattag acacattaga agaaggaatc aaattattgt
    47881 cttcggtcaa tgagaagatc gttacttccg tcatattgta tttagaggta gcctctgttt
    47941 ctattccgaa cttggtccga atttttatgg aaaagcatcc tgatatccaa ttaacgatca
    48001 tgcaacatca tttaccagat gatattaccg atcctgtact gtacatcact tcggagatga
    48061 agcctggcat gaccaatatt cctatcatga aagaacctgt ttatgtcgcc attcctaaga
    48121 aacatcctct tgctaaaaaa gaacatctgc aattagaaga tatccaacgc acccccatct
    48181 tgatgctgtc aaagagcaac gccttccgtc atacactgga tgcagcactt gaagcgaaaa
    48241 gtatcgaatt gacgatcagt tcgacgacag atgatcctgc gacattacgt tctattgtca
    48301 agcaaggatt agctgttagc tttttcccaa aagtttcttg gtcttacgat aaaaatgatc
    48361 ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg aacaatttat ttatcttcta
    48421 gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt
    48481 taggaaaata aaaaaaaaag agctgggaca aaattcttgt cgcagctctt ttttcgaaca
    48541 aacggtgttc cagaagtaat ttcttagaaa ataagccgaa atttcccaaa aatttgagaa
    48601 gcaattttcg taaattcctt cttatttctt gaagctaaac atttctgtca caacctcttc
    48661 tctattgtcc tagacattca ttgcgttgat ttaaaacttc catcaatcaa atctgctagt
    48721 tcatctagct tttctatttt acgtagatca atagagatcc aatgttcttt attcatgtga
    48781 taggctggaa aatacccctc ttttcctcgc aaaattccgc caagttcagg atcgaccttc
    48841 aaatcaagaa tattgatgat cctatggtca cttgcatcat catagatttt ttcttctgga
    48901 acagacatca ggaccccaaa ccattttttg cttttttgat gtctgaatac gcaataatca
    48961 ggaaattttt taaaaatata ttctggagac aaattgtatg tttcattgat gtgatcgatt
    49021 agttcttttt tcgttagcat ctgcttccat ctctttctta ttgattcgct ttattcaaag
    49081 ataccacgta agaatagaaa aatgaataca agtagttgct aatcaaatac ttcgatattt
    49141 ttctttttta tcaactctta aatgttcaat ccgcctgtgt tggtcttgct accatctact
    49201 attaaaatgt ttattattgt ttgcccacct attttttgat tttagtggta tcatttttca
    49261 agttcatgat gaaattctta tcatcagtaa atgctccaga aaaataaaca atgattgaaa
    49321 aatcagttga aacttgacca ccgtttattt tctccgtatt cttagaatag aagggagtct
    49381 gattgatgta ttcactaatg aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt
    49441 ctttagtaga acaattgctg aatcatacaa agataaccgt aaatgaatta gctgaaatca
    49501 tagggacaac cgaacgaacg attttttctg accttcaatc tattcgctct cagctgcccg
    49561 aaggctggga catcatcagc gatcaagcag gaatcagttt gcaaaaccag caaaatttat
    49621 taacaaatga cctttgggag atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa
    49681 atctgctttt tacaaaaaaa gttgctgtcc ctgattttct agccgaccat gggctttctt
    49741 atgggacatt gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc
    49801 agatcgattt gacgaaatat acagcttgta ttcttggaaa agagcgtgcg attcgtactt
    49861 tttatcatcg attgctgatt cctttcactc ataacaacta ttttttcgag gattattcga
    49921 tccatgaatc ccattatttt caatttttaa gaaacctcag tcaaactgaa ctagcggttg
    49981 aaacagaaga gattttcgga acatgttggt tctttatcaa tacgatccgt ataaaagcga
    50041 attgccgatt agattcttct atccatacca acagcacact ctcctccctg tatgactctg
    50101 cattgaaaaa attgtattta acggaaggta tttatcttaa agacactgag ctttcttttg
    50161 cttctttctg ctttctggaa agttggaatt acaacaataa ctatggacaa gaaaccgctc
    50221 gctgcctgca tcattctcca tttttagagg tcctcgaaac gtttgtagag gaactagctt
    50281 cagagctgtc tcttgatcaa ctaaaaaaaa catctctgac tgataacttg gctctcctca
    50341 tattaaaata tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt
    50401 ttttagaaac atatgatcag caatatgcac acttgtatca acagaaagag aatttactga
    50461 acgatctaaa aaaagaaata gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc
    50521 tactgatcca acaaacgatt ttttctatca aaccgaattt gttcaatgtc tattttattt
    50581 tccaaggaga accttcatgg aaggcgttct tgcagcagga attaaaagat tacttaggaa
    50641 aacgtgtctg cttcttacct atcgaactaa ccgatctttc cactatttct tttgaaaaaa
    50701 cagatatctt gatttccaat ttccctctag attctctaga tatccctatt gtatacattt
    50761 cttctattcc aacgaaaaat gagctcaatc gtttgactga actcactttc aaaacttttt
    50821 tatagtaacg gctagcgact gtattctcta gctgtttttt cttttttgcg aaacagtttg
    50881 ttcatgatcc ctttttctat ttctacaact aagaatagaa gaatcacatt tgctccaatc
    50941 aataactgtt gtgtcatcgt taaacttact gtaccgatca gctgctggcc gactggtaga
    51001 aaaataacca tcccttgcaa taaagcaagg atacctaatg aaagaaaaag cgctttgttt
    51061 tgcaaaagac cttggttcaa tgacggcttg gacaactctc tgcaattgat catgtaaaca
    51121 gcttgagcta agacgatatt ttgcagaaga atggtttgct gtaagctttg cccttcaaac
    51181 tgcatagcca ataaataggc aggaaccatg atcagtaggg agacatagat gatccgaaac
    51241 aaatgataac cagataggat tccttgcttt ggatctcttg gtgggcgctt catgatttct
    51301 ggatctggcg actcgaacga taaagtgatt gtcgttacca tattgaccca taaaatctgt
    51361 actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga
    51421 gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg
    51481 ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct
    51541 gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc
    51601 aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc
    51661 aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt
    51721 ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact
    51781 ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc
    51841 cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca
    51901 ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc
    51961 tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga
    52021 ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga
    52081 gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc
    52141 tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt
    52201 tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg
    52261 ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata
    52321 atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt
    52381 gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa
    52441 ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc
    52501 atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc aatcgtagcc
    52561 gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg
    52621 ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga
    52681 ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg
    52741 tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa
    52801 gccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca
    52861 gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt
    52921 aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc
    52981 agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc
    53041 atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg
    53101 agtccattga cgatcactac gaataaaata atacttcctt caatatattc actagaagca
    53161 aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct
    53221 atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc
    53281 aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat
    53341 acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg
    53401 ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa
    53461 ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata
    53521 caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt
    53581 cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc
    53641 tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag
    53701 accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac
    53761 tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa
    53821 taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt
    53881 cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca
    53941 aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt
    54001 gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata
    54061 aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg
    54121 cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa
    54181 ttccagcaaa actgaataaa accataggaa atgagactac cattccagca aagccaaacg
    54241 gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat
    54301 ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga
    54361 ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta
    54421 agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga
    54481 tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag
    54541 tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag
    54601 agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag
    54661 ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg
    54721 ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc
    54781 gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca
    54841 agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt
    54901 actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct
    54961 ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa
    55021 actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca
    55081 tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata
    55141 acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa
    55201 aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca
    55261 aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct
    55321 gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag
    55381 acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc
    55441 gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca
    55501 actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga
    55561 atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca
    55621 gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt
    55681 attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc
    55741 cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca
    55801 ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt gatgatccaa
    55861 taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg
    55921 ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt
    55981 tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc
    56041 caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag
    56101 attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt
    56161 gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag
    56221 gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat
    56281 agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca
    56341 tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg
    56401 gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg
    56461 gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc
    56521 tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc
    56581 gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc
    56641 atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt
    56701 ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc
    56761 ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat
    56821 ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca
    56881 gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac
    56941 ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg
    57001 agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca atggcaatac
    57061 acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata
    57121 agctcctaac atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga
    57181 aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata
    57241 gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc
    57301 atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc
    57361 agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata
    57421 gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc
    57481 tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac
    57541 agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa
    57601 agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc
    57661 gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca
    57721 tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat
    57781 ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag
    57841 ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat
    57901 cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc
    57961 aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc
    58021 ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat
    58081 cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta
    58141 acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa
    58201 gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct
    58261 aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat
    58321 cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc
    58381 cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag
    58441 atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga aatttttttt
    58501 tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg
    58561 ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac
    58621 ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac atatttttat
    58681 ttttttatat tgttaaaaat tcacaattta ttttttaata gaattatctg ttttccaaat
    58741 tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa attaactgat
    58801 gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa tacaaggcgg
    58861 aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc tgtttttatg
    58921 tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt
    58981 ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg aagaacagat
    59041 cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac atatacctcg
    59101 atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta
    59161 tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca tctctgtcag
    59221 caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg ggatcgaaat
    59281 cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc
    59341 gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg aacgtgccga
    59401 tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag aaaataaaga
    59461 aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg ctgtgacaat
    59521 agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc
    59581 atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct ttattttgac
    59641 actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg aagactttca
    59701 acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag ttgctacctt
    59761 tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg cgctgattca
    59821 tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat tttatctttt
    59881 aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca gctggtccag
    59941 ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg tgatcgaatt
    60001 gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca caaattcgga
    60061 tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg attttcaagt
    60121 tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc agtcctcaaa
    60181 ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg aaaatattat
    60241 tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa acaaataagg
    60301 ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat ttctgtaaca
    60361 atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag gggtccgatc
    60421 tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc tagttggaaa
    60481 atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac aagaatttcc
    60541 aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc gcttactcgg
    60601 tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat tgatagacaa
    60661 acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag tttttcgaaa
    60721 tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt ctttgttttt
    60781 ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg ataataataa
    60841 gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg atatcagcaa
    60901 cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt gtatctgcat
    60961 tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa agtttgaata
    61021 gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa atagcaatga
    61081 tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac tttcttttaa
    61141 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
    61201 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
    61261 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
    61321 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
    61381 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
    61441 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
    61501 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
    61561 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
    61621 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
    61681 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
    61741 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
    61801 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
    61861 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
    61921 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
    61981 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
    62041 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
    62101 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
    62161 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
    62221 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
    62281 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
    62341 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
    62401 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
    62461 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
    62521 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
    62581 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
    62641 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
    62701 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
    62761 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
    62821 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
    62881 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat
    62941 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
    63001 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
    63061 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
    63121 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
    63181 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
    63241 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
    63301 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
    63361 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
    63421 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    63481 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
    63541 gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga
    63601 gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg
    63661 caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga
    63721 agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga
    63781 aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact
    63841 attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac aactatttca
    63901 atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac
    63961 tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt
    64021 aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat
    64081 ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg
    64141 atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg
    64201 gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag
    64261 gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag
    64321 ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc
    64381 acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat
    64441 ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg
    64501 cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc
    64561 tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg
    64621 atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta
    64681 tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg
    64741 ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat
    64801 tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat
    64861 tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc
    64921 cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt
    64981 atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca
    65041 agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag
    65101 gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag
    65161 catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa
    65221 ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga
    65281 tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc
    65341 aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt
    65401 ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg
    65461 ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt
    65521 acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca
    65581 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
    65641 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
    65701 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
    65761 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
    65821 atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac
    65881 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
    65941 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
    66001 attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
    66061 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
    66121 aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc
    66181 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
    66241 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
    66301 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
    66361 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
    66421 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
    66481 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
    66541 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
    66601 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
    66661 ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
    66721 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
    66781 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
    66841 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
    66901 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
    66961 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
    67021 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
    67081 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
    67141 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
    67201 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
    67261 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
    67321 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
    67381 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
    67441 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
    67501 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
    67561 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
    67621 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
    67681 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
    67741 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
    67801 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
    67861 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
    67921 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
    67981 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
    68041 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
    68101 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
    68161 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
    68221 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
    68281 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
    68341 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
    68401 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
    68461 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
    68521 tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca
    68581 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
    68641 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
    68701 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
    68761 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
    68821 ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc
    68881 atcttgcacg attcctttct catccatata ctgttcttca tattctcttc ccataccgtt
    68941 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
    69001 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
    69061 aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg
    69121 ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
    69181 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
    69241 caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa
    69301 caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat
    69361 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
    69421 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
    69481 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
    69541 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
    69601 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
    69661 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
    69721 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
    69781 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
    69841 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
    69901 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
    69961 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
    70021 tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat
    70081 ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg
    70141 taataactgc accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg
    70201 gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa
    70261 tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga
    70321 tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca agggataact
    70381 tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga
    70441 tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata gtaataatgt
    70501 tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca
    70561 acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt
    70621 gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc
    70681 gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa
    70741 atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag
    70801 cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca
    70861 accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg
    70921 tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta
    70981 attcataaac agttggatta acagataaat aagacatcac tgcgatgata ccagggaata
    71041 caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg
    71101 tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa
    71161 aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca
    71221 atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca
    71281 ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt
    71341 tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct
    71401 ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt
    71461 accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg
    71521 tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg
    71581 gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc
    71641 tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa
    71701 agcaattgat tcattaatga ttgggataat atgcagcaag gctatgtttc cgctagcttc
    71761 tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa
    71821 agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata
    71881 ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg
    71941 tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg
    72001 cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag
    72061 agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata
    72121 ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt
    72181 gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc
    72241 tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtataa
    72301 aaaatcgatt aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac
    72361 taggataaaa ttcactccta gccttcgatc atttttattt tgatcgacaa aaaatatgat
    72421 gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata
    72481 tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc
    72541 ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg
    72601 acttgctccg atcagtacac ttgtaacaga tttttgtcgt aaattccatg caagggctat
    72661 ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag
    72721 agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc
    72781 ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt
    72841 gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg
    72901 agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat
    72961 ttcagtcatt tttttgtatc ttctccatta tagttggata taccaatata taatgctttt
    73021 ccttgtctta caagttgcat caacgcttct gctgtttctt ccaaaggtgt atcagggtct
    73081 gggcgatgat gatagaagat gtcaacataa tctagcccca ttctttggaa actttggtca
    73141 caacttgata tgatggattt acgagagccc cattccccat atggcccttc ccacatatga
    73201 taacctgctt tgctagaaat aatcagttca tcacggtagg cttgaaaatc ttctctaaaa
    73261 atacgcccaa agtttttctc tgcagctcca gcagggcctc cataattatt cgcaaggtcg
    73321 aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt atggatagga
    73381 tcataatcac caaaattttg ccataatccg agaggagaga taaaatcggt aattttaaac
    73441 ctgaatgacc ggatttacga tattccattt tttcataacg tgcactatct gcaatataca
    73501 atttttctcc tcctcatcaa atctttttaa aacgctttct tgttttctat ttttagttta
    73561 ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt
    73621 gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt ttttatagcg
    73681 tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc
    73741 tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt
    73801 tgattgttcc gctaattctt tttgtcgttc gtcttgataa aaaatcactg gacgatattg
    73861 cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat
    73921 tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac
    73981 atgacctgtg taaccaccac ttacttgatc ataattcggt ttttcaacat gtccgcccgt
    74041 gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca
    74101 gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt
    74161 ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat
    74221 tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttactt ctagttttga
    74281 taactgttct ttaacggata tattttctct tgtgtcattc tttgtctgaa tcaatacttt
    74341 tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat
    74401 ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg
    74461 taatttatca taaagtgagg aaataatcga ttgaatcgta tttattcaaa gaagataagc
    74521 agcttcagat gatataagat gatctttatc ttaaaaagca tactctatgg tttggtttaa
    74581 ctaaaaaacg acctctgaaa aaagggatgt tctccaattc tcagaagtcg agtaaagatg
    74641 attctttttt atgagcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
    74701 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
    74761 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
    74821 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
    74881 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
    74941 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
    75001 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
    75061 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
    75121 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
    75181 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
    75241 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
    75301 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
    75361 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
    75421 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
    75481 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
    75541 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
    75601 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
    75661 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
    75721 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
    75781 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
    75841 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
    75901 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
    75961 tagggtagct aacacacgag atgtacttac aaatgcagca tacaatgcag gcaatgacag
    76021 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
    76081 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
    76141 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
    76201 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
    76261 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
    76321 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
    76381 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
    76441 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
    76501 ataacaccga taataaatcc aataccaacg atttgcatcg cgacatcatt agagatacca
    76561 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
    76621 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaatgg tgcttcaatc
    76681 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
    76741 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
    76801 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
    76861 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
    76921 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
    76981 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
    77041 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
    77101 atagatatct gatccaccaa ctcttttgtc ggttctgaac gtgctcgaaa agctagtccc
    77161 aacaatacag cccagaataa aacgccaagg taattcgcat caataagaga ttgaacagga
    77221 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
    77281 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aatgactgct
    77341 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
    77401 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
    77461 gcgattgcct tcaagcaact gacaaacaac gtccccaata ttgaaataaa tgtccacgtg
    77521 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
    77581 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
    77641 atcattttat ctaaagaaac taaaggtgca attttctaaa aattctaata atacaattgg
    77701 caattcatgc tcttgcaaca ttcaatttta gtattggagg gtttattcaa tctctaaaga
    77761 agtcgagaga aaactattta tcgttattat tatagttatc caagtaaaac gtaactaaga
    77821 tgaaggacga aactagaaat ttcatccttc atcttaattc ttagacatag caatcaatta
    77881 atattttcca ctgagtctct tctttctctc actctcactg gtttacttac ccataaacga
    77941 tttgattaaa gccatttttc aattcattat tcgctgtctc gttaaagtaa agataattat
    78001 cgggattccc ctgattttgt tgtgtcacca atacttttgg ctgattttgc tgaaacccga
    78061 atagatagac gtgctttttc agatatggtt ctgtctctgc atcggaatag acggctgtga
    78121 tttgatagtc tgcttgacct gtcccacttt ctgaccattc tgcagaaaca ggcactccac
    78181 caatagccat cttccactcc ccgttcaaaa taacttgagg gactttaaga ccatataagt
    78241 ccacactcat ttgattcgta tagcttttgt attgttgtcc cattgtttgg ccccatgtag
    78301 ccataaatgc ctctaattgt gcggatttgt ctgcattcca cagtgtttgt tcgacttgtt
    78361 cgctgctttc tgaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
    78421 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttccgta cttgctgcag
    78481 ttggcttgct ctctgtgcta gttgttgtag tagttgcaat tgttgctttc gctgtttctt
    78541 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
    78601 caattgttac taaaatcctt tttttaaatt aaaaaactcc tttcgattac tcccacttct
    78661 ttattctatc aaataagcaa tgtgatttta ttgatttttt gtattttctc atccatcttt
    78721 taaacctttt aaaaactaag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
    78781 ttcttttatt ttaatcttcg cgattttcca atttacctca cgctttatat ttgtatttga
    78841 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
    78901 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
    78961 aattgtttca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
    79021 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
    79081 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
    79141 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
    79201 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
    79261 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ccaatacatg ccccaccatg
    79321 gcgccagcag ttggtttatg atgatcgata tctgcttgtt ttgtttcttg agcaaatttt
    79381 tcttccacta tattcactga tttacgcctc ttttactttt gttgattgga cttcataacc
    79441 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
    79501 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
    79561 cgcatgttct attttttgca tacagcttgg acaagctagt gtatctaatt gcataatcat
    79621 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
    79681 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
    79741 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
    79801 cccactagcc atgtaaataa acccggcaaa taagccgatg agtaaaaata agacgacacc
    79861 aatggcaatc actacatttt cacgggtatt caaaacagtt ttcttagcca aacggtaagc
    79921 atgaaccaac gatccaaaac tagattgcat caagacaacg tcactcgttt cgatagcgac
    79981 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    80041 gattccatct ccaatgaaag caacgtgcaa tccttgttct tggtattttt tcacaaattg
    80101 aaccttttca tctggcaata attcagcatg gacttcatca atgcctaaca tgtcagctac
    80161 ataatcagca gtcatctgat tatcacctgt cagcatcact aaatgttttg ctcctgcttg
    80221 tttcaacata gctaattgtt cagcgacttc tggtcgaatc acatctgaca caccaatgat
    80281 ttgagtgatt tttttgtcga ttgctacaat aatgactgag ctgcctcttt gttgtaattg
    80341 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    80401 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgatcgt
    80461 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    80521 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    80581 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    80641 tgtaccggtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    80701 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    80761 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgatgg ctaaacgaaa
    80821 atcacgactg atcagaccaa caagcaacgc aataagtaaa acggttggag tataatactg
    80881 agaaaattga tcaatgaatt tttccgcatg tgatttggta tcttgtgctt cttcgactaa
    80941 ttctataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcga tcgtcacgta
    81001 gccatctgct aaaatcgttc cagaaaatac gttatctcca ctgattttga cgacttgtcg
    81061 tgattcaccc gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    81121 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    81181 ttccacttct tcggtcgttc catcttcttg gacaacgctg gcagtcgatg gggccatctc
    81241 ggtcaaagcc ttgattgatt cccgcgtttt ggtcaatgtt ttttgttcca gataactacc
    81301 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    81361 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttta ctgcctgata
    81421 cgcatggatt ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    81481 gaacaagaca aatccagtaa atttcccgat aaaaccaaaa ataatcaaca cagctgataa
    81541 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    81601 tttccttttc attcttacga gactaagtat agatacttat ccatcataaa aaattgacca
    81661 ctatcaagtt tattcaaaaa ggcggttttc ttttggtata ctgaattcct acagtcatta
    81721 aacgatcata tagaaaaagg aagcgccgaa acgcttcctt aggtagttct atttttttat
    81781 tcaaagataa gatgctttgt catcttacga ctgaagaaca acaatcaaaa aagggcagca
    81841 gccactcatt cacaagaaat atcgtttttt gatatgggag ttgaagcagt ggacaaatat
    81901 ctctacttat tagctgggaa taagatccaa aaaagcttaa tggattttat ccaagaattg
    81961 gaatgtactt tccacaaaaa attcactcat tccattcttt taaaactttt gattcataca
    82021 gcgtgtctaa ttgagcgtac attgataaac ggtcatgaac ttaaaattat atcagaagat
    82081 gacacaagac cttctcatga aacaattttt catgtaaaaa aggccttcaa aaatattgaa
    82141 acagaatttg ggatcactgt ttcttatgat gaatgtttct ttatctatga tattatcgca
    82201 tctaaataat ctttttttat gtaaacgagt attttgggag cgtcaataat tttgtgtaaa
    82261 taaattgtcc tcctgcaaaa taattagtta ctcagtaaac attgaaacta atg
//
LOCUS       DACDBG010000003        76426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDBG010000003 DACDBG010000000
VERSION     DACDBG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.003"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..624
                     /locus_tag="IVZ90_000152"
     CDS             <1..624
                     /locus_tag="IVZ90_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8261231.1"
                     /translation="HIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNA
                     VMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTE
                     LLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRND
                     LTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            722..910
                     /locus_tag="IVZ90_000153"
     CDS             722..910
                     /locus_tag="IVZ90_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8261232.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(968..1321)
                     /locus_tag="IVZ90_000154"
     CDS             complement(968..1321)
                     /locus_tag="IVZ90_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8261233.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1852..2274
                     /locus_tag="IVZ90_000155"
     CDS             1852..2274
                     /locus_tag="IVZ90_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8261234.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2271..2501
                     /locus_tag="IVZ90_000156"
     CDS             2271..2501
                     /locus_tag="IVZ90_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8261235.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2999..3199
                     /locus_tag="IVZ90_000157"
     CDS             2999..3199
                     /locus_tag="IVZ90_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8261236.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3226..4419
                     /locus_tag="IVZ90_000158"
     CDS             3226..4419
                     /locus_tag="IVZ90_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8261237.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4487..6052)
                     /gene="guaA"
                     /locus_tag="IVZ90_000159"
     CDS             complement(4487..6052)
                     /gene="guaA"
                     /locus_tag="IVZ90_000159"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8261238.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6111..7034)
                     /locus_tag="IVZ90_000160"
     CDS             complement(6111..7034)
                     /locus_tag="IVZ90_000160"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8261239.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7230..7835
                     /locus_tag="IVZ90_000161"
     CDS             7230..7835
                     /locus_tag="IVZ90_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8261240.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8143..9159
                     /locus_tag="IVZ90_000162"
     CDS             8143..9159
                     /locus_tag="IVZ90_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8261241.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9300..10601
                     /locus_tag="IVZ90_000163"
     CDS             9300..10601
                     /locus_tag="IVZ90_000163"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8261242.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10647..11309
                     /gene="deoC"
                     /locus_tag="IVZ90_000164"
     CDS             10647..11309
                     /gene="deoC"
                     /locus_tag="IVZ90_000164"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8261243.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11330..11728
                     /locus_tag="IVZ90_000165"
     CDS             11330..11728
                     /locus_tag="IVZ90_000165"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8261244.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11794..12876
                     /locus_tag="IVZ90_000166"
     CDS             11794..12876
                     /locus_tag="IVZ90_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8261245.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13088..14650
                     /locus_tag="IVZ90_000167"
     CDS             13088..14650
                     /locus_tag="IVZ90_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261246.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14643..15776
                     /locus_tag="IVZ90_000168"
     CDS             14643..15776
                     /locus_tag="IVZ90_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261247.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15776..16732
                     /locus_tag="IVZ90_000169"
     CDS             15776..16732
                     /locus_tag="IVZ90_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261248.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16831..17997
                     /gene="deoB"
                     /locus_tag="IVZ90_000170"
     CDS             16831..17997
                     /gene="deoB"
                     /locus_tag="IVZ90_000170"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8261249.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18021..18839
                     /locus_tag="IVZ90_000171"
     CDS             18021..18839
                     /locus_tag="IVZ90_000171"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8261250.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18856..19563
                     /gene="deoD"
                     /locus_tag="IVZ90_000172"
     CDS             18856..19563
                     /gene="deoD"
                     /locus_tag="IVZ90_000172"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8261251.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19861..20547)
                     /gene="gpmA"
                     /locus_tag="IVZ90_000173"
     CDS             complement(19861..20547)
                     /gene="gpmA"
                     /locus_tag="IVZ90_000173"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8261252.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20719..21300)
                     /locus_tag="IVZ90_000174"
     CDS             complement(20719..21300)
                     /locus_tag="IVZ90_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8261253.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21433..22116)
                     /gene="rpiA"
                     /locus_tag="IVZ90_000175"
     CDS             complement(21433..22116)
                     /gene="rpiA"
                     /locus_tag="IVZ90_000175"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8261254.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22435..22848
                     /gene="rpsL"
                     /locus_tag="IVZ90_000176"
     CDS             22435..22848
                     /gene="rpsL"
                     /locus_tag="IVZ90_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8261255.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22932..23402
                     /gene="rpsG"
                     /locus_tag="IVZ90_000177"
     CDS             22932..23402
                     /gene="rpsG"
                     /locus_tag="IVZ90_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8261256.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23496..25583
                     /gene="fusA"
                     /locus_tag="IVZ90_000178"
     CDS             23496..25583
                     /gene="fusA"
                     /locus_tag="IVZ90_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8261257.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25755..26942
                     /gene="tuf"
                     /locus_tag="IVZ90_000179"
     CDS             25755..26942
                     /gene="tuf"
                     /locus_tag="IVZ90_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8261258.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27349..27657
                     /gene="rpsJ"
                     /locus_tag="IVZ90_000180"
     CDS             27349..27657
                     /gene="rpsJ"
                     /locus_tag="IVZ90_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8261259.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27688..28317
                     /gene="rplC"
                     /locus_tag="IVZ90_000181"
     CDS             27688..28317
                     /gene="rplC"
                     /locus_tag="IVZ90_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8261260.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28345..28968
                     /gene="rplD"
                     /locus_tag="IVZ90_000182"
     CDS             28345..28968
                     /gene="rplD"
                     /locus_tag="IVZ90_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8261261.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28968..29258
                     /gene="rplW"
                     /locus_tag="IVZ90_000183"
     CDS             28968..29258
                     /gene="rplW"
                     /locus_tag="IVZ90_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8261262.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29297..30130
                     /gene="rplB"
                     /locus_tag="IVZ90_000184"
     CDS             29297..30130
                     /gene="rplB"
                     /locus_tag="IVZ90_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8261263.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30173..30451
                     /gene="rpsS"
                     /locus_tag="IVZ90_000185"
     CDS             30173..30451
                     /gene="rpsS"
                     /locus_tag="IVZ90_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8261264.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30474..30821
                     /gene="rplV"
                     /locus_tag="IVZ90_000186"
     CDS             30474..30821
                     /gene="rplV"
                     /locus_tag="IVZ90_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8261265.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30835..31491
                     /gene="rpsC"
                     /locus_tag="IVZ90_000187"
     CDS             30835..31491
                     /gene="rpsC"
                     /locus_tag="IVZ90_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8261266.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31494..31928
                     /gene="rplP"
                     /locus_tag="IVZ90_000188"
     CDS             31494..31928
                     /gene="rplP"
                     /locus_tag="IVZ90_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8261267.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31918..32106
                     /gene="rpmC"
                     /locus_tag="IVZ90_000189"
     CDS             31918..32106
                     /gene="rpmC"
                     /locus_tag="IVZ90_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8261268.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32131..32397
                     /gene="rpsQ"
                     /locus_tag="IVZ90_000190"
     CDS             32131..32397
                     /gene="rpsQ"
                     /locus_tag="IVZ90_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8261269.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32455..32823
                     /gene="rplN"
                     /locus_tag="IVZ90_000191"
     CDS             32455..32823
                     /gene="rplN"
                     /locus_tag="IVZ90_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8261270.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32860..33168
                     /gene="rplX"
                     /locus_tag="IVZ90_000192"
     CDS             32860..33168
                     /gene="rplX"
                     /locus_tag="IVZ90_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8261271.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33196..33735
                     /gene="rplE"
                     /locus_tag="IVZ90_000193"
     CDS             33196..33735
                     /gene="rplE"
                     /locus_tag="IVZ90_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8261272.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33754..33939
                     /locus_tag="IVZ90_000194"
     CDS             33754..33939
                     /locus_tag="IVZ90_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8261273.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33975..34373
                     /gene="rpsH"
                     /locus_tag="IVZ90_000195"
     CDS             33975..34373
                     /gene="rpsH"
                     /locus_tag="IVZ90_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8261274.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34405..34941
                     /gene="rplF"
                     /locus_tag="IVZ90_000196"
     CDS             34405..34941
                     /gene="rplF"
                     /locus_tag="IVZ90_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8261275.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35102..35458
                     /gene="rplR"
                     /locus_tag="IVZ90_000197"
     CDS             35102..35458
                     /gene="rplR"
                     /locus_tag="IVZ90_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8261276.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35479..35979
                     /gene="rpsE"
                     /locus_tag="IVZ90_000198"
     CDS             35479..35979
                     /gene="rpsE"
                     /locus_tag="IVZ90_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8261277.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35994..36173
                     /gene="rpmD"
                     /locus_tag="IVZ90_000199"
     CDS             35994..36173
                     /gene="rpmD"
                     /locus_tag="IVZ90_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8261278.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36217..36657
                     /gene="rplO"
                     /locus_tag="IVZ90_000200"
     CDS             36217..36657
                     /gene="rplO"
                     /locus_tag="IVZ90_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8261279.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36657..37952
                     /gene="secY"
                     /locus_tag="IVZ90_000201"
     CDS             36657..37952
                     /gene="secY"
                     /locus_tag="IVZ90_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8261280.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38014..38661
                     /locus_tag="IVZ90_000202"
     CDS             38014..38661
                     /locus_tag="IVZ90_000202"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8261281.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38852..39070
                     /gene="infA"
                     /locus_tag="IVZ90_000203"
     CDS             38852..39070
                     /gene="infA"
                     /locus_tag="IVZ90_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8261282.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39103..39219
                     /gene="rpmJ"
                     /locus_tag="IVZ90_000204"
     CDS             39103..39219
                     /gene="rpmJ"
                     /locus_tag="IVZ90_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8261283.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39237..39602
                     /gene="rpsM"
                     /locus_tag="IVZ90_000205"
     CDS             39237..39602
                     /gene="rpsM"
                     /locus_tag="IVZ90_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8261284.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39630..40019
                     /gene="rpsK"
                     /locus_tag="IVZ90_000206"
     CDS             39630..40019
                     /gene="rpsK"
                     /locus_tag="IVZ90_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8261285.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40099..41037
                     /locus_tag="IVZ90_000207"
     CDS             40099..41037
                     /locus_tag="IVZ90_000207"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8261286.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41065..41445
                     /gene="rplQ"
                     /locus_tag="IVZ90_000208"
     CDS             41065..41445
                     /gene="rplQ"
                     /locus_tag="IVZ90_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8261287.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41717..42556
                     /locus_tag="IVZ90_000209"
     CDS             41717..42556
                     /locus_tag="IVZ90_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8261288.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42532..43401
                     /locus_tag="IVZ90_000210"
     CDS             42532..43401
                     /locus_tag="IVZ90_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8261289.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43391..44188
                     /locus_tag="IVZ90_000211"
     CDS             43391..44188
                     /locus_tag="IVZ90_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8261290.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44391..45137
                     /gene="truA"
                     /locus_tag="IVZ90_000212"
     CDS             44391..45137
                     /gene="truA"
                     /locus_tag="IVZ90_000212"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8261291.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45470..48139)
                     /locus_tag="IVZ90_000213"
     CDS             complement(45470..48139)
                     /locus_tag="IVZ90_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8261292.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48377..48565)
                     /locus_tag="IVZ90_000214"
     CDS             complement(48377..48565)
                     /locus_tag="IVZ90_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8261293.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48835..49197
                     /locus_tag="IVZ90_000215"
     CDS             48835..49197
                     /locus_tag="IVZ90_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8261294.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49249..50931
                     /locus_tag="IVZ90_000216"
     CDS             49249..50931
                     /locus_tag="IVZ90_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8261295.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            51048..53084
                     /gene="recG"
                     /locus_tag="IVZ90_000217"
     CDS             51048..53084
                     /gene="recG"
                     /locus_tag="IVZ90_000217"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8261296.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            53133..54134
                     /gene="plsX"
                     /locus_tag="IVZ90_000218"
     CDS             53133..54134
                     /gene="plsX"
                     /locus_tag="IVZ90_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8261297.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54256..54498
                     /gene="acpP"
                     /locus_tag="IVZ90_000219"
     CDS             54256..54498
                     /gene="acpP"
                     /locus_tag="IVZ90_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8261298.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54766..55770
                     /locus_tag="IVZ90_000220"
     CDS             54766..55770
                     /locus_tag="IVZ90_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261299.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55771..56715
                     /locus_tag="IVZ90_000221"
     CDS             55771..56715
                     /locus_tag="IVZ90_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261300.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56715..57677
                     /locus_tag="IVZ90_000222"
     CDS             56715..57677
                     /locus_tag="IVZ90_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261301.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57704..58618
                     /locus_tag="IVZ90_000223"
     CDS             57704..58618
                     /locus_tag="IVZ90_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261302.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58644..60425
                     /locus_tag="IVZ90_000224"
     CDS             58644..60425
                     /locus_tag="IVZ90_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8261303.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60773..61459
                     /locus_tag="IVZ90_000225"
     CDS             60773..61459
                     /locus_tag="IVZ90_000225"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8261304.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61479..65060
                     /gene="smc"
                     /locus_tag="IVZ90_000226"
     CDS             61479..65060
                     /gene="smc"
                     /locus_tag="IVZ90_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8261305.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            65069..65878
                     /locus_tag="IVZ90_000227"
     CDS             65069..65878
                     /locus_tag="IVZ90_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8261306.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65889..66887
                     /gene="ftsY"
                     /locus_tag="IVZ90_000228"
     CDS             65889..66887
                     /gene="ftsY"
                     /locus_tag="IVZ90_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8261307.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            67074..69341
                     /gene="gshAB"
                     /locus_tag="IVZ90_000229"
     CDS             67074..69341
                     /gene="gshAB"
                     /locus_tag="IVZ90_000229"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8261308.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69430..69882)
                     /locus_tag="IVZ90_000230"
     CDS             complement(69430..69882)
                     /locus_tag="IVZ90_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8261309.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            70032..70982
                     /locus_tag="IVZ90_000231"
     CDS             70032..70982
                     /locus_tag="IVZ90_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8261310.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70975..71934
                     /locus_tag="IVZ90_000232"
     CDS             70975..71934
                     /locus_tag="IVZ90_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8261311.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71931..72686
                     /locus_tag="IVZ90_000233"
     CDS             71931..72686
                     /locus_tag="IVZ90_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261312.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72709..73662
                     /locus_tag="IVZ90_000234"
     CDS             72709..73662
                     /locus_tag="IVZ90_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8261313.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74306..74602)
                     /locus_tag="IVZ90_000235"
     CDS             complement(74306..74602)
                     /locus_tag="IVZ90_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8261314.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74957..75313
                     /locus_tag="IVZ90_000236"
     CDS             74957..75313
                     /locus_tag="IVZ90_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8261315.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75323..76222
                     /locus_tag="IVZ90_000237"
     CDS             75323..76222
                     /locus_tag="IVZ90_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8261316.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        24888 a        13818 c        16394 g        21326 t
ORIGIN      
        1 cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt
       61 ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca
      121 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
      181 aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact
      241 ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga
      301 acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga atatctttca
      361 cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat
      421 aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat
      481 ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt
      541 accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga
      601 tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat
      661 taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt
      721 tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat
      781 acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac
      841 tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca
      901 gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt
      961 ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta
     1021 aggatagttc tttgtctgta aagctatcca tgtatttttc tacctgtaac cttcgagtgc
     1081 ttttaatcaa attatctgta ggtaagaaaa attcatcaat agatacatgg agtaatgata
     1141 caagatcata cagcacttgt gtacttgggt gttgtccttt gttttctata tttgttaaat
     1201 aacgtgggtc aatttcaatc attgttccca cttgttcacg agtgagaccc cgtttcattc
     1261 gagcctcttt aatcgctaaa ccaacggctc taaaatcgta tttatcttct tttttacgca
     1321 tactaaatca cctctataca ttttactgtt cctatataac cataaacagg tatagaaaaa
     1381 catgttatat agtttatgag ttcctataga acactgtgga atttattaat gcgatgaatc
     1441 caatttcaaa tagaaaggtc gctgatttta tttacctata caactttata aaatttagtc
     1501 atactcctag aaagtagata aaattggcga cttaatggag ggaaaagtcg ccatgagaaa
     1561 gataatctgt catctagttc tcttaattaa gttcttatca aaaaaactta tccacctgta
     1621 ctggatagat atgaccatca atgtggtttt gcagattaca tagatagaaa aatcaaagca
     1681 tatgaacaga gaaaacaatc tgtttatgtg cttttttggt cacttaataa atatctttta
     1741 ggatttctct tcatttatgc aacaaatccc ccgttcgtgt cggggtttag tatgaaggga
     1801 gataaagaag cacatgccac tccctttcac gacagaaagg gggtgaaaag catgaaacca
     1861 tcttcttttc aagaaaccat agaaaatcag tttgactata tctgcaaaca agttattgaa
     1921 gatgaacgaa aagattactt caaacatcta gacagactat cagcaaaaga agtctcgttt
     1981 tctgatatag ataactacct tgtcaactct ttttctactg ttgatacgta cgtcactgat
     2041 ttccaactat ttaaattatt tggattaaga atcaatgttg aaagtgattt attaagtgaa
     2101 gccctgcgtc atctttcaga gaaaaaacgg aacattattt tacttcatta ctttatggat
     2161 atgagtgatg tggagattgc ggagttacta aatttgaatc gttcgactgt ttatcgtcat
     2221 cgaataagtg gactagccat gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag
     2281 cttatccaag ggttcccttc cccctcattg taaaggcgac agatggcgac gtggaagcaa
     2341 ttaatcagat tgttaaacat tatcgagggt atacctcaaa acgttcatta cgtcgcatga
     2401 cagacgaata tggaaattct catatggtta ttgatgaaac cctacgtggc agaatggaaa
     2461 cgaaactcat ctctaagatt ttatcgttcg aaattaaata gtcccatttt ttcgtggaag
     2521 cgtgtcgaat cgtatccacg cttcccgatt tgggaggttt gttattccat caaagcatat
     2581 tgtgtgttca gtaggttttg ataggctaac aagccaaacg ttctttgaca accaaataaa
     2641 ggcaatcgaa tacgtttcgg taagaaaaga gctgatgaac taacacgcca tgacctacaa
     2701 aattagtgag cgattcatgt tagtaattcg aaagtaatct ttagcaggat tgctgccatg
     2761 actttcttat cgtgataatg atactcccgt atagccatag tccgagcgtt agaagcgtca
     2821 caggcaatgg gtacggctac atgagaacca tgcagaagtg aaattcctgt gagctttgct
     2881 agagctgttc gattgctaag atattcttct cttgtcagtg tattattata aaactatttg
     2941 atataattat aggtgaatat tggagttttc ctagcctata attgggaaag gagtaataat
     3001 gataacggtc acaaaaaagg atttagttga gctaggatat ggaacttctt tttcagcaga
     3061 catcataaaa aaagcaaaag aattgatgat tacaaaaggg catatctact atcaatctcg
     3121 aaagctagat agagtccctc gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa
     3181 tgaaaacagg tctgagtagt ttttgcacag taaggagtaa aaactatggt taaagaccca
     3241 ataaaaaaag caaagaatgg aacctattat tttcgtgcaa atctaggtta tgattctaaa
     3301 gggaaaaaaa ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc aagagaagaa
     3361 tattcgaaac tgctattgat gaaaccagaa gaattaagtg agaataagga taaaatgacg
     3421 tttaaacatt atatctttga aattttcctt ccatggtata agacacaggt aaagctcaga
     3481 acatatgaaa atcgcttacc aactattaag aagcatttct cttattttga taaaatggct
     3541 gtttccgata ttgagcctat tgatgtgcaa aattggcaac taaaattatc aaagaagtgt
     3601 aagtcttctt atgtaagagc tgttcaagga ctattttcag ttgctatgga tcgagcaatt
     3661 gtccttggga taaccaccac aaatccatct aagattattg gcaatgtgaa aaagcaaaag
     3721 tctaaaattg aattttggac aaaagaagaa tttgaaaaag taatctctct aatttacaaa
     3781 gaagattact atcaacactt tttatttatc tctctgtggt ttttatttat gactggaatg
     3841 cgaattggtg aagctactgc cattcaatgg gaagatattg attttgactc aggagtacta
     3901 accatcaaca aaacactcta ttacaagaat caatcgaatt atagatttgt tgaaccaaaa
     3961 acaaaggcga gcgttcgtca tattgtatta gataaatgca cattaactta tttatccgaa
     4021 tggaaggaag tacaacaaaa tctaattcag acagactttg taatgagcta taacggtatt
     4081 cctacccaaa aacatacact atcatatgct attgaacgct atgctaagct ggctggtatc
     4141 catcgaatca gaatccatgg gttacggcat tctcatgctt ccttgttaat cagtatggga
     4201 gaaaatccac ttatcattaa agatcgtcta ggacacgaag atattgagac gactttagga
     4261 acgtatggac atctttatcc taacagtaat ttcgaggtag ctaacaaatt aaatggagcc
     4321 atttccttta aagaagcaaa acaaaattat gatacttcac caaagaatca atttactgta
     4381 gactacttgc gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga
     4441 agagccagaa acccttggta taagaggatt tctagctctc ctacaactat tcccactcca
     4501 ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt
     4561 ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg
     4621 tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt
     4681 cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac
     4741 ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt
     4801 ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg
     4861 gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt
     4921 ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac
     4981 ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag
     5041 tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt
     5101 cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag
     5161 atacaccttt caacttgtct aagaagcgat ctcttgcatc gactttgatg atgttcaagc
     5221 cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt
     5281 ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga
     5341 cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac
     5401 ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac
     5461 aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg
     5521 catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat
     5581 ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc
     5641 tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta
     5701 cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt
     5761 tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga
     5821 tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa
     5881 tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt
     5941 cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt
     6001 agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta
     6061 cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt
     6121 cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt
     6181 cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct
     6241 tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct
     6301 ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc
     6361 agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc
     6421 tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg
     6481 atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt
     6541 atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa
     6601 ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa
     6661 agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc
     6721 gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata
     6781 ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat
     6841 tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca
     6901 tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa
     6961 ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc
     7021 attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa
     7081 ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat
     7141 agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg
     7201 tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag
     7261 atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg
     7321 taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg
     7381 atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt
     7441 atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca
     7501 ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg
     7561 ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg
     7621 taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat
     7681 atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc
     7741 cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata
     7801 aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg
     7861 aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt
     7921 ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt
     7981 ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata
     8041 aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt
     8101 tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga
     8161 gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa
     8221 ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat
     8281 tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg
     8341 atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat
     8401 ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag
     8461 atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca
     8521 aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt
     8581 ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag
     8641 ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg
     8701 aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg
     8761 aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa
     8821 ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc
     8881 gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc
     8941 ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg
     9001 caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg
     9061 atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta
     9121 attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg
     9181 taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag
     9241 tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa
     9301 tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa
     9361 tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat
     9421 tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg
     9481 cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata
     9541 agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta
     9601 gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac
     9661 tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac
     9721 gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg
     9781 acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg
     9841 ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga
     9901 ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca
     9961 tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag
    10021 aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac
    10081 aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg
    10141 ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg
    10201 aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag
    10261 cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta
    10321 aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc
    10381 taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc
    10441 ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg
    10501 acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg
    10561 caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta
    10621 ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt
    10681 tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc
    10741 atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag
    10801 gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga
    10861 caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca
    10921 tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag
    10981 ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag
    11041 atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca
    11101 aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg
    11161 aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag
    11221 caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg
    11281 tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact
    11341 caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc
    11401 ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa
    11461 cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc
    11521 agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc
    11581 tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc
    11641 ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc
    11701 ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa
    11761 aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta
    11821 ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact
    11881 gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca
    11941 ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg
    12001 aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa
    12061 tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt
    12121 attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac
    12181 tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa
    12241 gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta
    12301 gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa
    12361 gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat
    12421 gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa
    12481 aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca
    12541 aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa
    12601 tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca
    12661 ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc
    12721 cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga
    12781 caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt
    12841 gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga
    12901 cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc
    12961 cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa
    13021 cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga
    13081 aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg
    13141 aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt
    13201 gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga
    13261 gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa
    13321 agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac
    13381 tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa
    13441 aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga
    13501 tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact
    13561 ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga
    13621 aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt
    13681 gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg
    13741 tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat
    13801 gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt
    13861 cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa
    13921 cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg
    13981 gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa
    14041 gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg
    14101 acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa
    14161 tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc
    14221 agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga
    14281 acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga
    14341 agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg
    14401 tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt
    14461 actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca
    14521 tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct
    14581 attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc
    14641 acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt
    14701 taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt
    14761 attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc
    14821 aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt
    14881 tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac
    14941 ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct
    15001 tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg
    15061 aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac
    15121 atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa
    15181 atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag
    15241 gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt
    15301 taggatttga aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga
    15361 gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg
    15421 gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta
    15481 ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt
    15541 tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag
    15601 gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca
    15661 acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg
    15721 ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc
    15781 gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat
    15841 tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga
    15901 gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc
    15961 acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt
    16021 ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac
    16081 agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa
    16141 aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga
    16201 tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat
    16261 tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg
    16321 ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg
    16381 ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca
    16441 gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc
    16501 tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg
    16561 ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc
    16621 agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg
    16681 gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag
    16741 cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc
    16801 aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg
    16861 gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat
    16921 acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt
    16981 ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg
    17041 acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg
    17101 ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc
    17161 aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca
    17221 gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact
    17281 tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta
    17341 tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga
    17401 atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat
    17461 gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag
    17521 gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct
    17581 gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat
    17641 ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg
    17701 gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt
    17761 gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg
    17821 tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg
    17881 aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag
    17941 aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct
    18001 ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt
    18061 ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag
    18121 gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct
    18181 ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca
    18241 agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag
    18301 tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg
    18361 catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata
    18421 tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat
    18481 ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag
    18541 aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg
    18601 aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga
    18661 ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta
    18721 ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa
    18781 ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat
    18841 aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag
    18901 attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat
    18961 ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa
    19021 cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa
    19081 ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca
    19141 aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg
    19201 atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg
    19261 aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg
    19321 gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt
    19381 ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag
    19441 acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca
    19501 gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa
    19561 taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag
    19621 ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt
    19681 ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa
    19741 cagcttttca tgtttgatga atattgtgac ttgtgaccga gaagttggca ggagaaaacc
    19801 gtttatacga aaaaagggac tgtgacaaat cgtgtcacag tccctttttt atagtgtttt
    19861 ttataggtag tattttttag caactgttaa gtcatcattt aattcataaa caagtggttg
    19921 acctgttgga atttcaagat ccatgatgtc ttcgtcagag ataccttcga tatgtttagc
    19981 tagggcgcgt aatgagttac catgtgcagc tactaaaaca gttttgttgt ctagtaatgc
    20041 tggagcaatt tcatcttgcc agaatggcaa agcgcgttct aatgtcactt tcaagttttc
    20101 tccaccagga atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc
    20161 ttcgtcagtt tcttccatta atggaggaag agtatcgtaa gaacgacgcc agatgtgcac
    20221 ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc aaaccttgta atttaccata
    20281 gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc caaagttgat ctgaatattc
    20341 taagatcaag ttacatgttt tgattgcacg agttaaaaca gaagtgtaag caacatcaaa
    20401 ttcgattcca gcttctttga ttttgcgtcc gccttctttt gcttcttcga ttccttctgg
    20461 tgctagattt acatcagccc aaccagtaaa ttggttcaat gcattccatt cactaagacc
    20521 gtgacgagaa aaaactaatt ttggcatgtt tagctctcct tttaagatag gtttatattt
    20581 ctgaaatttt cagttctgtt ttattgtaca attttttgga agagatttcc atcgaaaaat
    20641 tagataagat tctatgagaa aacaagtaga gaatataaaa aaacgaacag tgcccacgca
    20701 actgttcgtt tttaggtatt agatgcataa ttgctcgatt tctttttcag gtaaattgat
    20761 atatttataa catgttccaa ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc
    20821 ttttttttca taatagagac gtcggatttt tttgatcgtt tttgcatcga tttttggtct
    20881 tccgccgatt tttccttttt tacgagcttc atctaatcca actaatgtac gttctttgat
    20941 caatgcgcac tccatgtttg ctaggttttc cattaattgg aaatagattt ttcccatttc
    21001 gcgcgtatcg attccttcat caagactaat caagtgcagc ccgcgatctt tgaataattg
    21061 cgtgagttct gttaattgcc gcgtagattt gcctaatcga tgaagttggc aaatgactag
    21121 tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa tgaacagcag tagaatcatt
    21181 ttgttccact tcatatgtct catggaaaat ttcttgacaa ccaaagtcag ataattgatc
    21241 aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa taattttcat
    21301 ggtgattcct ttctagcagt gattttctaa ctggatataa cagtaaaaat gtagcataaa
    21361 gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt ctttttttat tggatgatca
    21421 agcagttttt ctttatttac gacggattgc gtcaagaatc tcaacgcctt tttctgagcc
    21481 gacaactact ttagaaacca tgtttaggaa aagcccatgt tctactaccc caacaagttc
    21541 atctaaatag gtagctaagc tttctggctg ttcgatgact tctagatgca gatcaatgat
    21601 gtaatgacca ccatctgtgg ttaagacttc tccgttttcg tctgtacgca agacgggttg
    21661 gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag ccataaggga tcacttcaac
    21721 tgggagtggg aacttcccaa gtttatcgac taatttagaa gaatcaacga tccaaatcgt
    21781 ttcttttgag tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat
    21841 cccttgaaaa tcagcactga tttcatcagc accgtctatt gtcaaatcga ctacggggac
    21901 ttcatcaata ctctttaacg gtattcctaa tttttccgct tgttcttttg tcgcatttga
    21961 agtagtaact cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa tctcttctac
    22021 catataatag gcggttgaac cggtcccgag acctacgatc atcccgtcct ttacgaattt
    22081 cgcggcttca atgccagcta attgttttaa attcatcttt gcgtccacct ttttttagtt
    22141 tgagcatcgt gctttcctca ctattttagc gtgatttacg gtaaaaagat accattcttt
    22201 tttattccaa taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt
    22261 cgtgatattc taacaaaggt gctttatcta caggtttctg tgaagaaacg tgctgactgt
    22321 ttatcaaagc atatttgata aggatgcaga cattgcattt attttttatc acaaaaaaag
    22381 aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct
    22441 acaattaacc aattagtacg taaacctcgt aaatcaaagg tggaaaaatc taattcacct
    22501 gctttgaaca aaggatataa cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa
    22561 aaacgtgggg tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta
    22621 cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag ttactgctta tatcccaggg
    22681 attggtcata acttacaaga acacagtgtt gtattattac gcggtggtcg tgtaaaagac
    22741 ttaccagggg tacgttacca tatcgttcgc ggtgctttag atactgctgg tgttaacgac
    22801 cgtaaacaaa gccgttctaa atacggaact aaaaaaccta aagcttaatt ttaattatta
    22861 aacaaatgaa acttgcttct actatataga agtaattact atgaacaata tttcggaagg
    22921 aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta
    22981 tttataactc aaaattagta actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg
    23041 ggattgcagc taacattatc tataatgcct ttgacttgat caaagaatct acaggtaacg
    23101 atccattaga agtgtttgaa caagcaatga aaaacgttca acctgttctt gaagttaaag
    23161 cacgtcgtgt tgggggttca aactaccaag taccagttga agttcgccct gaacgccgcg
    23221 ttactctagg tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg
    23281 aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa
    23341 aacgtgaaga cactcataaa atggctgatg ccaaccgtgc gtttgctcac tatcgctggt
    23401 aagattctct ctgtttggtc ggcttttata agtcgacctc agatagtgtc cttattttta
    23461 gcaactagat agtaaagaga ggagaaacaa ggagaatgac cagagaattt tctttacaaa
    23521 acactcgtaa tatcggtatc atggcccaca tcgatgctgg taaaacgact gctactgaac
    23581 gtatcttgta ctatactggt aaaatccata aaattggtga aactcatgaa ggagcttcac
    23641 aaatggactg gatggaacaa gaacaagaac gtggtattac gatcacttct gctgctacta
    23701 cagcacaatg gaagggtcac cgaatcaaca tcatcgacac accaggacac gtagacttca
    23761 cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac
    23821 aatcaggtgt tgaacctcaa actgaaacag tttggcgtca agcaacaact tatggtgtac
    23881 ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc tgacttctta tattcagtat
    23941 caactttaca tgaccgtttg caagcaaacg ctcacccaat ccaattacca attggtgcgg
    24001 aagacgactt cacaggaatt atcgaccttg taacaatgaa ggctgaaatg tatacaaacg
    24061 atcttggaac agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag
    24121 aatggcgcga aaaattggtt gaagctgtag ctgaaactga tgaagaactt actttgaaat
    24181 acttagaagg tgaagaaatc actgaagctg agttaaaaga aggtatccgc cgcgcaacag
    24241 ttaacgtaga attctaccca gtactatgtg gttcagcatt taagaacaaa ggggttcaat
    24301 tactattgga cgctgttctt gattacttgc catcaccgtt agatatccca gctatcaaag
    24361 ggattgaccc taaaactgat gaagaagtag aacgtcctgc agacgacagc gctcctttct
    24421 cagctttagc atttaaagtt atgactgacc cattcgtagg tcgtttaact ttcttccgtg
    24481 tgtactctgg tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg
    24541 aacgtgttgg tcgtatccta cagatgcatg ccaactctcg ttcagaaatc tctgaagttt
    24601 acgctggaga tatcgctgct gctgttggtt tgaaagacac aactactggg gacacactat
    24661 gtgatgaaaa gagtctagtt attcttgaat ccatggaatt cccagaacca gttatccaag
    24721 tcgctattga acctaaatca aaagctgacc aagataaaat gggtgtggct ttacaaaaac
    24781 tatctgaaga agatcctact ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca
    24841 tcgctggtat gggtgaactt cacttagata tcatcgttga ccgtatgcgt cgtgaattca
    24901 aagttgaagc taacgtaggt gcgcctcaag tatcttaccg tgaaactttc cgtgctggca
    24961 ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat
    25021 ggattgaatt tacaccaaat gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg
    25081 gtggggttgt tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa gatgctatgg
    25141 aaaatggtgt cttagctggc tatccattag tagatatcaa agctaaactt tacgatggtt
    25201 cataccatga tgtcgattca aatgaaactg ccttccgtgt tgctgcttct atggcattac
    25261 gtgcagcagc taagaaagct aatccagtaa tccttgaacc aatcatggct gtagaagttg
    25321 ttatccctga agattattta ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg
    25381 ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg gttccacttg
    25441 ctgaaatgtt tggttatgct acaactcttc gttctgctac tcaaggtcgt ggtacattta
    25501 caatgacatt tgaccactac gaagatgtac caaaatcagt acaagaagaa atcattaaga
    25561 aaaacggcgg aaaagctgaa taatttctta aaaccggata gtcctgcaaa gagattatcc
    25621 aaagagacta tatttttaat atagtttcgt gtaaaataat tatcgatgat atgggcatgt
    25681 ggtgtacctt gtgtacatca tatacctatc agacaaataa aaaatatatt gtatttttag
    25741 gaggacattt taaaatggca aaagaaaaat ttgaccgttc taaaccacac gttaacattg
    25801 gtactatcgg acacgttgac catggtaaaa ctacattaac agctgcaatc acaactgtac
    25861 tatctaagaa aaacggcggc caagctatgg cttacgatca aatcgatggt gctccagaag
    25921 aacgcgaacg cggaatcaca atctcaacag ctcacgttga gtatgaaaca gatacacgcc
    25981 actacgctca cgttgactgc ccaggacacg cggattacgt taaaaacatg atcactggtg
    26041 ctgctcaaat ggacggagct atcttggtag tttctgctgc tgacggccca atgcctcaaa
    26101 ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga
    26161 acaaagtaga catggttgat gacgaagaat tactagaatt agttgaaatg gaagttcgtg
    26221 acctattaac agaatacgaa ttccctggtg acgatgttcc tgtagttgct ggatcagctt
    26281 tgaaagctct agaaggcgac gcttcatacg aagaaaaaat tcttgaatta atggctgcag
    26341 ttgacgaata catcccaact ccagaacgtg acaacgacaa accattcatg atgccagttg
    26401 aagacgtgtt ctcaattact ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac
    26461 aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa
    26521 cagttactgg tgttgaaatg ttccgtaaat tgttagacta cgctgaagct ggagacaaca
    26581 ttggtgcttt actacgtggt gttgcacgtg aagacatcca acgtggacaa gttttagcta
    26641 aaccaggtac aatcacacct catacaaaat tctctgcaga agtatacgtg ttgacaaaag
    26701 aagaaggtgg acgtcatact ccattcttca ctaactaccg tccacaattc tacttccgta
    26761 caactgacgt aacaggtgtt gttgaattac cagaaggaac tgaaatggtt atgcctggcg
    26821 acaacgtaac aatggaagtt gaattaatcc acccaatcgc tatcgaaaac ggtactaaat
    26881 tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa atcaaagctt
    26941 aattcactta agttttaaag taaagcaaca tttattcgga cgtaatcgct cggaacgaag
    27001 gtcttaggat ctttgttccg agctttttta tttattgcag gatcaggaaa aaagacagga
    27061 gtattataaa tagaagaaac acgaaataaa agagtcagta ttttctaaat gaaactttat
    27121 gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt gagtttatat atattataat
    27181 gacaaaggtg cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct
    27241 aagaaacgag aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt
    27301 ttcgtggagc tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa
    27361 aaaattcgta tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa
    27421 atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact
    27481 gaacgcagtc tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa
    27541 ttcgaaatgc gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt
    27601 gatgctttga tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac
    27661 taaaacaaaa ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga
    27721 atgacacaaa tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact
    27781 ccaaacgtag tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt
    27841 ggttaccaag atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa
    27901 gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat
    27961 gaagtaggaa aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca
    28021 ggtactacga aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga
    28081 ccaatggctc acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct
    28141 aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc
    28201 caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat
    28261 gttcctggag ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc taaataattg
    28321 taggaagaaa ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc
    28381 caaaacggtg aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt
    28441 gtttacgatg caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa
    28501 aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt
    28561 gcacgtcaag gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca
    28621 acaccacgtt cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct
    28681 gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac
    28741 gcaccaaaaa caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta
    28801 ttggttgttc tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac
    28861 gtttctgtag taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg
    28921 ttagcaacac aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag
    28981 acgtaatcaa acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat
    29041 acacttttga agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg
    29101 cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta
    29161 tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag
    29221 attcaaaaga aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa
    29281 attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa
    29341 catgacaggt tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca
    29401 accattgaaa aaccatgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg
    29461 tggcggacac aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt
    29521 tgcaactgtt caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca
    29581 ctatgaagat ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa
    29641 acttgtttca ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat
    29701 tccagttggt actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat
    29761 ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg
    29821 cttgaactct ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt
    29881 aggtaacgaa caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg
    29941 taaacgccca actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg
    30001 tgaaggtaaa gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct
    30061 tggttacaaa actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa
    30121 aaataaataa ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg
    30181 tagtttaaag aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc
    30241 aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag
    30301 ttttgttgga tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca
    30361 agaagacatg gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca
    30421 tgctgctgac gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag
    30481 aacaaatcac atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc
    30541 gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat
    30601 tcacaccaaa caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg
    30661 cagaaaataa ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg
    30721 aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca
    30781 aacgtacaag tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt
    30841 caaaaagtac atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg
    30901 tatgctgaaa aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt
    30961 tcaactaaac ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc
    31021 gtaaacattt caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa
    31081 gtcgaaagcc taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc
    31141 gtggaaatca aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa
    31201 ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt
    31261 gctggcgctc aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct
    31321 cgttcagaag gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat
    31381 tacgcatggg aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat
    31441 cgtggagaaa ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag
    31501 tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag
    31561 gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca
    31621 caaaccgcca aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga
    31681 aagtatggat taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta
    31741 tgggttctgg taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca
    31801 tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc
    31861 acaaattacc aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg
    31921 aaggttaaag aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta
    31981 aaagaagaat tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca
    32041 cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct
    32101 aagtaattat ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt
    32161 tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca
    32221 aagaaaaacc accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat
    32281 gatgaaaaca acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta
    32341 tcagctacaa aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa
    32401 cacgagattt tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc
    32461 caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc
    32521 aaagtccttg gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg
    32581 gttaaacaag caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc
    32641 gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat
    32701 gctgcagtga ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt
    32761 gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta
    32821 taatcttgag acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat
    32881 aaagttaaag ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc
    32941 ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct
    33001 tctcaagctg ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac
    33061 gtgatggtgg ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc
    33121 aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa
    33181 ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca
    33241 ttgatggaaa aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc
    33301 atcaacatgg gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa
    33361 gaattaactt tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct
    33421 ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga
    33481 atgtacgaat tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat
    33541 ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa
    33601 ttgatcttcc cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt
    33661 attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg
    33721 ccattccaaa aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa
    33781 aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt
    33841 ccacattcag tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat
    33901 aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag
    33961 gaggtaaaag atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta
    34021 acgcaaacat ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca
    34081 tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca
    34141 aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa
    34201 acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa
    34261 aagtattgaa cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata
    34321 aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta
    34381 gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct
    34441 gaaggagtaa cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa
    34501 ttgactcgta ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc
    34561 actcgtccaa acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc
    34621 aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt
    34681 gggtaccgtg cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca
    34741 gtagaaatca caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt
    34801 gttaaaggtg cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt
    34861 cctccagaac cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa
    34921 gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa
    34981 aggcatttgg ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta
    35041 tttttcgaaa cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat
    35101 tgtgattaca aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa
    35161 caatatctct ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat
    35221 ctacgctcaa attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga
    35281 taaagaaatt tcaggtggaa caaaaacaga agcagcacaa gctgtcggta aactagttgc
    35341 tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg gataccttta
    35401 ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa
    35461 gaaggaggaa taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc
    35521 gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct
    35581 gctttagttg ttgtcggtga caaaaacgga cacgtagggt ttggtacagg taaagctcaa
    35641 gaagttcctg aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta
    35701 cctatggttg gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc
    35761 ctaatgaaac ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta
    35821 ttagaattag ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc
    35881 aacgttgttc gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca
    35941 gaacttcgcg gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg
    36001 aattaaaagt tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag
    36061 ttaaagcgtt aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa
    36121 ttaaaggcat gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa
    36181 aaatcataac tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct
    36241 gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga
    36301 aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta
    36361 ggatttgaag ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac
    36421 gtgaaccgta aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga
    36481 gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga
    36541 atcaaagtat tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc
    36601 tctaaagcag ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt
    36661 ttagactatt aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg
    36721 tatttgtgtt gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg
    36781 cttcattgca aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta
    36841 gtgcgatgca aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta
    36901 tcgtcattca gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag
    36961 gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat
    37021 ttatccaatc tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga
    37081 ataatcctaa cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt
    37141 ttgttacttg gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga
    37201 tcatttttgc aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt
    37261 actttataaa tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac
    37321 tagtagcggt cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga
    37381 tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga
    37441 aagtaaatgc agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa
    37501 atgctgtttt acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga
    37561 tggagatctt taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg
    37621 ccttcacttt cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc
    37681 aaaagcaagg aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct
    37741 caagattact gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc
    37801 ttccaatcat tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa
    37861 gcttactgat cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc
    37921 tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca
    37981 cacgttctca cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca
    38041 ggcgcaggta aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc
    38101 tcaacaggag atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca
    38161 aagtcttata tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa
    38221 gagcgactag ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg
    38281 ctagatcaag caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct
    38341 gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc
    38401 tgcagcaatt gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca
    38461 tgtgatcgtt gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt
    38521 aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa
    38581 caaggtttgc taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta
    38641 caaaaaatca ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc
    38701 ttgtcaagtt caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag
    38761 atgatccatt ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa
    38821 aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga
    38881 aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca
    38941 ccaagttctt gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg
    39001 tgacaaagta acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg
    39061 ctttaaataa ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca
    39121 gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt
    39181 tgcccagcaa atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg
    39241 ctcgtattgc aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata
    39301 tctatggtat tggtaacact actgctaaaa agatcttagc tgatgctggt gtatctgaag
    39361 atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata
    39421 aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg
    39481 aaatcggttc ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca
    39541 cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat
    39601 aattcttaag tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc
    39661 gtgtgaagaa gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta
    39721 tcatcatgat cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag
    39781 gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta
    39841 caaaagcagc aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt
    39901 ctggacgtga agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc
    39961 gtgacgtgac tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat
    40021 gagtgccgct cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg
    40081 aaaggggtaa agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa
    40141 gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta
    40201 gggaattccc tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt
    40261 caaatcgatg gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact
    40321 caaatcatct tgaacattaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc
    40381 cttgaaatcg atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt
    40441 gatgtagaaa ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc
    40501 catgctcgct taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa
    40561 gaagatatgc caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg
    40621 aactatcaag ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg
    40681 gagatttgga cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc
    40741 atgactgaac atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc
    40801 atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta
    40861 gacttgtctg ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa
    40921 cttacaaata aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta
    40981 gaagaagtta aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac
    41041 cttttacgaa ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa
    41101 cgtaaagcga tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact
    41161 actgaagctc gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa
    41221 cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc
    41281 gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat
    41341 attgcacctc gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca
    41401 agacgcggtg atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac
    41461 atcactttat agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct
    41521 agctcagcgc tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt
    41581 ttttttatac cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt
    41641 taagcgagaa ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga
    41701 tgaagaagga gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca
    41761 gccagatgat cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg
    41821 gatcgctatt attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg
    41881 attgcttctg ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa
    41941 tatttggacg atccgacaga tggttggaat gatttttcaa aatccagaca atcagtttgt
    42001 tggttcgaca gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga
    42061 agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt agaatggctg agtttgcttc
    42121 acgcgagcct gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt
    42181 tgccttacga cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg
    42241 tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat
    42301 atctatcact catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca
    42361 aggcgaactt gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat
    42421 cgacttaggt ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg
    42481 ggttgatgtg cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc
    42541 cgttttgaac aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta
    42601 tttgacatca atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga
    42661 agcgggaaat caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca
    42721 gtccatattg gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc
    42781 cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta
    42841 gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc
    42901 ctagcaaaag aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca
    42961 tttgaacttt caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg
    43021 ccggaagtac ttgtattgga tgaaccaacg gcaggacttg atccccaggg cagaaaagaa
    43081 atgatggaga tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg
    43141 catttaatgg atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga
    43201 atcgttaata gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa
    43261 caactaggcg tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac
    43321 tttgctacct tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga
    43381 ggaaatgccc atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat
    43441 ccatcgattg gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt
    43501 tttagcaaat aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt
    43561 cttgtcaaaa gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat
    43621 cctcttcact gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg
    43681 gggtgttttc catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt
    43741 cgtgttgatc atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc
    43801 ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat
    43861 ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa
    43921 gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa
    43981 aatgaaagca gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga
    44041 agatcttgcg actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata
    44101 tcgtgtattg cattggcata atagagacac gatggttatc attgcttttg tattattaac
    44161 tgtgctactg atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt
    44221 gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt agggtctgtg
    44281 gcagactaag gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaattttat
    44341 agaaaaatca ttattccaga taacctttta ggaacaaagg agagaaaaac atggtacgtt
    44401 acaaagcaat cattgcgtac gacggaacaa attttaatgg attccaaaaa cagccaaatg
    44461 ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa
    44521 tcacggtctt tggttcggga aggacagatg ccggagtaca tgcgatcggg caagtaatcc
    44581 attttgatta tccagaggag cgtccgttag aacgaatgcg tttcgcgcta gatacccagt
    44641 caccagagga tattgctgta agacaagtag aaattgtttc agaagacttc cacgcaagat
    44701 atttggtcaa agaaaagacg tatcaatttc gagtggatat agggaaacca cgcagtcctt
    44761 ttcgacgcca ttatgcaagt tattttcctt acccacttga tttggaaaaa attcagcggg
    44821 cgcttcctga tttattaggg actcacgatt tcacctcttt ttgtgcatca ggaagctcga
    44881 tagaggataa aatacgtacg atctacgagg ctaagatgga ggtaaatgaa acaaaagacg
    44941 aattgttgtt tacatttagg ggaaatggct tcttgtataa gatgatacgg atcttagtag
    45001 gaacgctttt gaaaattggc aatggccgat tgcctgaaga tagtatccca gatattattg
    45061 cgaaaaaaga tcgtaatgca gcaggaccta cagcacaccc tgaaggactt tatttgtatg
    45121 aagtgaaata tgaataagag gttatgatag aagtggtcag cttcaagaaa taagaaggta
    45181 tttacgaaaa ttacttctca aaattttgca aaatttcggc ttattttcga agaagctact
    45241 tctggaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaaaga
    45301 gcaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt
    45361 gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt
    45421 gttttgattg cccttggtgc ggacagtctg aacaggttcc atcgtttctt tattttctac
    45481 gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc tggatcaatc gtgccataaa
    45541 aggggcgatt gcggtcaagg taccagacat aaagaataaa caatgcaata cataggacaa
    45601 aaaggattgc acctatcgta aatccttcag gcaagtaact gaaagtgaca gtatgtttac
    45661 ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt gctgaccttg actggtttcc
    45721 catcaatttt agctttccat cctttgtcat aaggaatagt tgtcaccaac atggaatctt
    45781 cagcggtatt tacagtccca gaagcttttc tgccagtggc ttccatttcg acaccttttt
    45841 ctttgatctg attcattgcg gcttgatagg ctacaacatc tagaccaacg actttaggct
    45901 ccatgaaact tatttctttc gtcccataaa aacttgcagt aaatgtcact gttgtctctt
    45961 gaggataata gccaagatca tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg
    46021 taccattgac agtgatcgtc gcactagaac tttctaattg cgcaaaattg gttgggaaca
    46081 aacttaagta agcttgtgta tttgcaggca catggacttc ccaagtgagc tcttttggct
    46141 ggttatctgc tgtttctgag taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca
    46201 actgattctt caaagtaggt gtatagaatg taaaatactg ttcatttaga ccagacaatg
    46261 tattgatcaa gtttgtctga ctggtcaaat tatctgtcac tggctgctca acatcattaa
    46321 cggacaacgg tgcagtaaat gctagtggca atgcattgac attctgatac agtgtatagt
    46381 cgccgctttg accagcttgg cggaagccat atttactgaa cgtgctttcg tttttagaaa
    46441 tgttatattt gattcctgta aacccatcca tcagcaaagt attattgtta taacggatat
    46501 tcaaatttgt ccctcttgaa cggaaaccta atttatccag gtaagaggaa gaattacgat
    46561 ttcttataga agaaaacaaa ctgataccac tgtaaccata gttgatcgaa tcgtttgcag
    46621 atactggatc cagattttcc aagcggtaaa acgagtcgga atcttttttt gtttcattta
    46681 caagagcttt caagtcagga taaggatcag tatataaact gcgggaagca taattccaat
    46741 catcaagaat gccatggatc atactgttcg tattgaccag tgcttcagca gacatgatca
    46801 ataataacaa tatactcaga tgattgaatg aaagtttttt atgctgataa aatcccacag
    46861 caaaagcata aatccctaag aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg
    46921 tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa gagagcagct acgaccaaaa
    46981 tgattcctaa caacagccca ccatcgtctt gttctatctt ttcccatcct tttcctgcaa
    47041 gcaggagcac taagaaagag aacagatagg cataacggaa taaaaacata ttcggtgcat
    47101 gcataccatg ccagaacaaa ttcaatggaa caagataaaa acttgcgata agtatggcga
    47161 aaagactgcc gtacaaaaat ttatttttca acggtacttt ccgactggca aaataaaaca
    47221 gacagaggac taatggtagc agacctgcgt aaacgaacgg aatcgatccg tattttgtcg
    47281 tgtcataaac accaatcata tttttcatga ttagatccag ataagaagtc gcttctgttt
    47341 tcaacgtagt gatctgactc agtgcctcgc catttgaccg aagatccagc actgctggca
    47401 atacaatgat cattgaagcg ccgcctgcca gtaaagaagt gataccataa ggaacaatcg
    47461 tttttttata ttctgaccag tttgtcaata gacgggcgat aaaatataag aaagaaaata
    47521 cgccaatcat aaaacccata taaaaactgg ataaaaacaa gagtaaataa ctgataaata
    47581 aaagtttcgg ccgtttaaga tccataacac gatgaatccc caaaatcact aatggaagat
    47641 aaacaaatgc atccagccac atgatgatct ccgaatgtgc agtgataaat gagatcaatg
    47701 cgtaactgac gctaagtgca acatgccccc agcgaggaat cttgaacgta cgtgcaagaa
    47761 accaaaaagc caatccggca cagccgacct taagcaaagt cagaacatac agtgcatctg
    47821 gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa gatcccgcct agatagtaag
    47881 aaattaaaga taaataattc aatcctagag aagcattcca tgtataaaaa atactttgct
    47941 ttccaagaag aacatttcgg aagctggcat gaaaattaga aaactgtgaa aaggcatcac
    48001 ttgccaaaat acttctgctg cttccaggat agattccgat acttagataa acaagtgcta
    48061 aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg tccgttttct ctaaagaatg
    48121 actttaaatt ttttttcata tttattttcc accaaatttc tatgaagttg tattcagttt
    48181 atcatatcct tgtttttctc tgcttaaaaa gagtctagtt ttcacaaaaa aataaaattt
    48241 agaataaata acttcataat ttgttcgtat aacaaaaata aacgcaaaaa aagggtagga
    48301 atcaaaagtc ccacccttaa aaatttttat taaataaaaa accgttcact atctgaacgg
    48361 tccaagtatc attcaattaa acgcgttcaa cttttcctga tttcaaagca cgagttgaca
    48421 cccaaacttt tttaggtttt ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa
    48481 cagtacgttt tgttgagttc atagcgtgtg aacggttgtt tccacttttt gtcttgcgac
    48541 ctgtaaagta acaaactttt gccatggtaa tttcctcctt tgccttgatt tggagcaata
    48601 gaataattca gctcatacca gactaacata tcatatagaa tgggaatttg caagtacatt
    48661 ttcatagata caattgacaa ttgagaagaa taattgtaaa ttaaacaagg ttatacctga
    48721 aattttgcaa ggtttttgag tactttgggt aactgccttt tatttcaagt tgcgctatga
    48781 taaaatatga tagaagaaaa atgggagttt tttacccgtt aaggaggctc attcatggct
    48841 gtaaaaattc aaacgtcagc tggaaccatt gaaattacca atgatgtaat tgcaacagtt
    48901 gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt
    48961 aaagataata ttactgagat tttgcgtaaa gaaaattact caagaggagt agttgtccgc
    49021 caagaagata atggtattgc tgtagatgtt tatacgatta tcagctatgg tacgaaaatc
    49081 tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc attattaggc
    49141 gtaactgcca attcagttaa tgtctttgta caaggtgttc gtgtactgcc agactaggca
    49201 ggagactggc tggtaactca gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca
    49261 gaaataagtg caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta
    49321 aatgcagaat acgtcaattc attaaatgta ttccctgtgc cagatggcga tacgggaaca
    49381 aatatgaatc tatctatgac tagtggagct aaggcagtag tagattcaac ctcagaaaaa
    49441 gtaggagaat tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc
    49501 ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc aaattccaga tgttgtctca
    49561 ttaaatgctt ctgatcttgc tgcagccttc actcatggag ttgaaactgc gtataaagca
    49621 gtaatgaaac cagtagaagg aacgattttg acagtagcta gagaagcagc gaaagctgga
    49681 gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga
    49741 gcaaagcgtg ctttagctaa aactccagac ttgcttccag tcctaaaaga agtcggtgtc
    49801 gtagacagtg gagggcaagg actgctgttt gtttatgagg gctttttaag tgcattgaat
    49861 ggagaattcc aagcggatga aacatatgaa ccttctccag cagaaatgga tgatatggtc
    49921 aatgcagaac atcatcgcag tgtccaaggt caaatggcga cagaagatat ccattttggt
    49981 tactgtacag aaatcatggt aaaaatcgga gaaggaccta cagtcgatag tacattcgat
    50041 tacgaagaat tccgtaacta tttagatggt ataggagatt ctttacttgt ggtcaatgat
    50101 gacgagatca tcaaagtcca cgtgcataca gaaaacccag gagaagtctt gaattatgga
    50161 caaaaattcg gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg
    50221 attttggaac acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg
    50281 tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga aagtttaggc
    50341 gctaattatg ttatcagtgg tggacaaaca atgaatccaa gtacggaaga cattgttaaa
    50401 gcgattcaag aagtaaacgc agataaagtg attgtcttgc caaacaataa gaacatcttc
    50461 atggcagctg atcaagcagc agaagtagca gatatcccag tagcagttgt tccttcaaga
    50521 acgatttccc aaggattgac agctatgctt tcattcaatg atcaagcttc tttagaagaa
    50581 aacaaagaaa cgatgacaga aatgttgtct agcgttgtga gcggtcaagt cactcacgcg
    50641 atcagagata cctcaattga cggtgtagag atcaaagaag gagacttttt gggcatgatc
    50701 gatggaaaaa tcctgatctc taacccagat atcacagcaa cagcgttaag tacactagaa
    50761 caaatgatca atgaggacac agagatcgta acgatcttgg caggtgatga aggaacagtt
    50821 gaacaagcaa atgaatttgc tgagaaacta acagaaaaac atcctgattt ggaaatcgag
    50881 atccaccaag gagatcagcc tgtctatcct tatctatttt ctgctgaata atttctgtgt
    50941 cagaatatct ttctaacaca gtattcattc ataaaaggcc ttcggttagt gatcaggtat
    51001 gatcgctgac caagtccttt ttttgttaaa ggagggagga aaaatttatg cgttctttag
    51061 atgatccaat cactatgctg gcaggagtcg gagaaaaaag agcagctagt cttgccagct
    51121 tgggcatcca aacgattgaa gacttactga cttattatcc ttttcgctac gaagatattc
    51181 aggaaagaaa tctaaacgag atccaggatc aagaaaaggt tactttgaaa ggaatcgtag
    51241 tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga
    51301 tgcaagaaca tgatgtgttc aacgtttctt tttttaatca accttatcta aaagaaaaag
    51361 tcattttatc ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt aaagccttga
    51421 acgggatgaa gattttaggt tctcaaacaa tggatgactt ttcgccgata tatcacgtca
    51481 acaaatcaat tcgtcaaacg acattagttg atctaatcaa aaaagggttt caagaatacg
    51541 gcacattgat tgaagaaaat ctgccaaaca accttgtgga gaaatatcga ctgcttgacc
    51601 gagcttctgc tgtaagaagt atgcattttc caaaagacca cgaagaaaac catcaagcca
    51661 aacgacgtgt cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa
    51721 aagcagaaaa agcagaaacg aatggtttgg agatccttta tgatgtccaa aaattgaaac
    51781 aatttactca aaagctacct tttgaactga caaatgcaca aaaacgtgta acgaatgaaa
    51841 tctgtcggga tcttcgcagt ccgcagcata tgcagcgact tttgcaggga gatgtaggta
    51901 gcggaaaaac agtggttgcc gcgattgcgt tatacgctac cgttacagca ggtttccaag
    51961 gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc
    52021 tgtttgatcc caatgaagta accatcgcat tgattacggg gtcaacgaaa gcaaaagaac
    52081 gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg
    52141 cattgatcca agatggagtc gaatttcaga atttaggtct agttattacg gatgagcagc
    52201 accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct
    52261 tatttatgac agcgactccg attcctagaa cattagctat tacagcatat ggtgagatgg
    52321 atgtatctat cattgatgaa cttccagctg gacgtatccc gatcgaaaca cgctgggtaa
    52381 gaacacctca attggacagt gtactggatt ggacgaaaaa agaactggca agaggacatc
    52441 aaatgtatgt catctgtccg ctgattgaag aatcagaagc attagatgta aaaaatgcag
    52501 tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac
    52561 atggtaaaat gaagaatcag gaaaaagaag taatcatgga agccttcaaa gacaaccaga
    52621 tgcagatatt agtttcgact actgtaattg aagtaggggt aaacgtgccg aacgcgaccg
    52681 tcatgctgat catggatgcg gatcgatttg gattggcaca gctgcaccaa cttcgtggac
    52741 gtgttggacg cggaagtgat gcttcctact gtatcttagt tgctaatcct aaaaatgaac
    52801 taggtgtgga acggatgaaa atcatgacag agaccaccaa tggttttgta ctaagcgaaa
    52861 aagatctgga attacgtggt cctggtgaag tatttggctt ccgtcaatct ggtctgcctc
    52921 aatttgcgat tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc agagaagagg
    52981 ctgcagccct ttggcaaata aaggattggc agatgcttcc tgagtatcag cctttagcgc
    53041 aaagcctcca aatagaaaat caggataatc gttatttcga ttagcagact gtgctatact
    53101 ttatactgat gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg
    53161 gagatcacgc acctcaagca attgtagaag gcgtggcttt agcgcagcaa gattttccag
    53221 aagtagaatt cttactttac ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa
    53281 aaaacatcac gattatccat acagacgaaa aaatcaatag tgatgatgag ccagtaaaag
    53341 caattcgacg aaaaaagact gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg
    53401 aagcagatgc tgtattttca gcagggaata caggcgcgct actagcagca gggttgttca
    53461 tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg ccagtagtag
    53521 ggaaagaagg tgccggtttc gatatgctgg atcttggcgc taacgcagaa aacaaaccag
    53581 aacatttgtt gcaatacggt attttaggtt ctttttatgc acgtaaagtc cgaggtatcg
    53641 gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga
    53701 cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt gaattttatt ggaaacgtag
    53761 aagctcgtga gctactcaat ggtgtggctg atgtagtggt cacagatgga tttacaggca
    53821 acgctgtttt gaaatctatt gaaggaacag caatgaatat gatgaatcta ttaaaatctg
    53881 ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac
    53941 gttctttgaa agcagaaatg gattattcca aacatggcgg tgccgttttg tttggcttga
    54001 aagcaccagt cattaaaact catggagcaa caggccccga tgctgttcgc tatacgatcc
    54061 gccaaatcca cacaatgctt gagacagatg tagttggaca attagtcgaa caatttgaaa
    54121 aaagcgaaga atagtatttg gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac
    54181 attcctgaaa aatgccagta aaattagcca tggtgtaggt aattgacaag tgtatgattc
    54241 ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa aattatttca
    54301 aatcattttg agatagatac cgataaagtg acggatgaat tgaacatcaa agatgattta
    54361 aaagctgatt caattagtat tatggaattc gttttagagc ttgaagatga attcggtacg
    54421 gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt agattatatc
    54481 agcagccatc ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct gcatgcagct
    54541 ttttatcctt ctttttttaa tctttttgac acgtaaaagt catttttctg ataattctgg
    54601 cgataaacag aaattatgtt gcaaagtaaa gcggttttct atataattat taataattca
    54661 ttacacaaag ataagaataa cctttgttat aaaacgattt attctgtgta atgtcctttt
    54721 ttattaagaa aaacttccat ttaggaagca gagaggggta ttcgattgtc cgataaccaa
    54781 ttattagaag ttcagaacct acatacagga ttccgactca aagatgacta ttacgatgca
    54841 gtagatgacg tttcatttac tttagacaaa aacgagattt tagcaatcgt tggggaatct
    54901 gggtgcggga aaagtacctt ggctactacg attatgggat tacttgatcc taataacaca
    54961 aaaattacag gtgagatttt atataacgat ctgaatttaa tcgacctgaa cgaaacgctc
    55021 tataataaaa tccgcggaaa tgatatcgga atgattttcc aagatccgtt gtctgcatta
    55081 aatcctctca tgagaatcga agaccagatc aaagaaggac tttcctatca cacaaagatg
    55141 aatgcagaac agcgtcaagc tcgtgcacta gaattgctag aacaagtagg gattcctaat
    55201 cctgcgagag ttggacgcca atatccccat gaactatcag gcggtatgag acaacgtgtc
    55261 atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca ttgcagatga accgacgaca
    55321 gccctagatg taaccatcca agcacagatt cttgatttac tgaaagacct tcaggaagaa
    55381 acaaaaacag ggatcatctt gatcactcat gacttaggag tcgtagcaga aatggctgat
    55441 cgagtggcag tgatgtatgg cggacagttc gttgaagtag caggtgtaaa agaactgttt
    55501 gatcatccta agcatcctta tacacgctct ttactgaact ctataccaca agaaggaaat
    55561 catgatgcag aactccatgt gatcgaaggt gtcgttcctt ctctgaaaaa catgcctcgt
    55621 aaaggttgtc ggtttgcacc acgtattcca tggataccag caagcgatca tgaagaagag
    55681 cctatccttc atgaagtcgc accgaaccac ttggtacgtt gctcctgcta taagcatttc
    55741 cactttagag acgaaaaagg agaggtgtag atgacagaat taatcacaat caaagacctg
    55801 aaagttcatt accctattcg cagtggattc ttcaatcgag taacggatca tgtatatgct
    55861 gtagatggtg tggattttat tatcgaaaaa ggaaagacat atggtttagt gggagagtct
    55921 ggctctggaa aatctactac gggaaaagcc gttgtgggat tagaaaaggt aacagccgga
    55981 caaatcatgt atgaaggaaa agatgtaacg aagcgaagca accgcaaaaa aatgggttat
    56041 aacaaggatg tgcagatgat cttccaagat tcaatgtcca gtttgaatcc taaaaagcga
    56101 gtgttggata tcattgctga acccatccga aattttgaac ggttaagcga tcaagaagaa
    56161 aagaaaaagg taaaaggact gctagatatc gttgggatgc cggaagatgc gctatataaa
    56221 tatcctcatg aattttcagg cggacaacgt caacgtctag gagtagcccg tgctgttgct
    56281 accaatccaa aactgatcgt agccgatgaa cctgtatctg ctctggatct atctgttcaa
    56341 gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat acggcctcag ctatttattc
    56401 atttcacatg atttaggtgt agtaaaacac atgtgcgaca atatcgcaat tatgtacaaa
    56461 ggtcgatttg tagaaatcgg tacaagagaa gacatttata atgacccgcg acatatctat
    56521 acgaaacgtc tgctttcagc tattccgcaa atcgatgtag aaaataggga aagccacaaa
    56581 gaaaatcgac gaagagtaga acaagagtat atccaaaacc aaaaagaata ctatgatgcg
    56641 actggtcgag tctacgattt gcgtacgatc acaactacac acaaagtggc gttgaaagat
    56701 ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt tttattgatg atcccgcaag
    56761 ttattatttt aagtgttctg atcttcttat tagcccaagc gatgccgggg gatccattta
    56821 ccggtttgat caatccaaat caggatccgg cagtgatcga acagatgcga caagcagctg
    56881 gcttgaatga cccatggtat gtccagtact tccgttggat cggaaatgct ttgcaaggcg
    56941 attttggtca aagtttctta tacaaacgct ctgttgctga cttgatcggc gaacggatca
    57001 tcaacacttt atatctatcg atcttgacag ttgtccttac ttatttgatt gctgtgcctc
    57061 tggggatgct tgctggccgg tatcaaaatt caatcttgga taagattgtc gtaatctaca
    57121 acttctttag ttttgctgtt cctttattta tcttcggctt gatcatgcta tttgtttttg
    57181 gttatcgttt aggctggttc ccaacaagtg gatcacaaac actaggtttg caaggtggat
    57241 ttatcactca atggatagac cgtttgaaat atattctgtt accatcaatt acccaagcat
    57301 ttttggcgac tgccgtcaca atccaatatc tgcgaagtga agtaatcgat tcgaaatcgc
    57361 tcgattttgt cagaacagcc cgttccaaag gggtaccgac gaataaagta ttcagtcgac
    57421 atattttccg aaatgcagcg ttgccgatgg cttctcaaat ggggtatgaa atcacttcgt
    57481 tgattgctgg ttctgtagtt atcgaaaaga tttttggtta tccgggagtc ggaaaactat
    57541 ttattgattc catcggccaa cgggactata ccgtgatcac atccttagta ttgattttag
    57601 gaatcgcgac gctagtgggg acattacttt cagatatcat catgagtatc gttgacccgc
    57661 gtattcggat tcaataaaac caatcagaaa ggagaatgga tcgatgagtg aaaaaaacaa
    57721 tacagcagtt caagaaagta ttcctcctat ggggatacga atgattgctc gagaatttaa
    57781 aaaagataaa attgctattt tttcattagg tttgctagtg gttttattat tagtgatctt
    57841 tatcggtgcg atgctgaccg atcaagacaa agtgatgtat gttagtatct tcgacaaata
    57901 cgctgcacca ggaacagttt cgacacaagg acaaaagttc attctgggag cagatgaagg
    57961 cggacgtgat gtattaggac aattgatcat aggtgctaga aactctgtta cgatcggatt
    58021 tgcgatcacg atccttactt cgatcatcgg ggttggatta gggatcatct ctggctacta
    58081 cggagggatc attgacaaca tcttgatgcg gatcgttgat tttatcatga ttctaccgat
    58141 catgctgatc attatcgtct tcgtatcgat tatttccaga tacaatgtct ggtcctttat
    58201 attcatcatg agtgcatttt attgggtagc taaagcccga ttattccgaa gcaagacatt
    58261 atctgaagca agaagagatt atgtcagtgc ctcaaaaaca atgggaacca atgattttaa
    58321 aatcatgttc cgcgaaatca tgccgaatct aagttcatta atcattacta atttgacgat
    58381 gaactttgcg gcaaacatcg gtatcgagac aactttgact ttcttaggtt tcggcttgcc
    58441 ggcaaatgta cctagtttag gtacattgat cggttatgca agtaacggag aagtcttagt
    58501 gaatcggcaa tggatctggt tacccgcatc aatccttatt ttagttttga tgttgagtat
    58561 caattatgtt ggacaagcat tcaagcgttc tgcagatgcg cgccaacggt taggctagta
    58621 tagaagcgaa gggggaaata ggaatgaaga aaacgatttt tggatttgca gcgctgatgt
    58681 ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga tacctctgga aacaactctg
    58741 gaggcaacgg caataacaaa gaggaattaa cttttccaat tgcaacaaaa aataatgatg
    58801 atgcaatcga agacggcgaa ttagaagctg cggtcgcaac ggacagtcag tttaaaggat
    58861 tgttccagtg ggagttttac caagattcat atgatgcagc ttttatggct ccttctcatg
    58921 aaccgctatt tgcaaacgat gacgatttca gaatcacaga cgacggagct gcaacgcttg
    58981 atctagatga agatacgaaa aaagcgacaa tcaaagtaaa agacaacgtc aaatggtcag
    59041 atggtgaaga tgtaacggcg gacgatgtga ttttccctta cgaaatcatt gggaataaag
    59101 attacactgg tatccgttat gacgacactt tccgtaacat cgtaggtatg gaagaatatc
    59161 atgatggaag tgccgacaca atctccggaa tcaaaaaaat agacgacaaa acagttgaaa
    59221 tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg tggtggtatc tggtcatatg
    59281 cagcaccaaa acatacgtta gaaggtgtag caatcaaaga tatggaatca agcgatcaag
    59341 tacgtaaaaa tccagtgact tttggccctt attacatgag taatatcgtt gcaggagaat
    59401 cagtagaata cttgccaaac gagcactact ggaatggtac gcctaaattg aaaaagatca
    59461 cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt gaactctaaa ttatatgata
    59521 tgattttcca aatgccaaca gatacttacg atacctataa agaaattgct ggctatacaa
    59581 acttaggccg tgaacaaact tcttatactt atctaggatt caagttaggt aaatggaatg
    59641 cagatacaaa gagtgtggaa tacaatccag attcaaaaat ggctgataaa tcattacgtc
    59701 aagcaatggg ctatgcatta gataataatg cagtaggtga acgtttctat gcaggtttga
    59761 gaagcaatgc gacttcattg attcctccag tattccagtc ataccatgat gatgagatga
    59821 aaggattcac acaagatcta gacaaagcga aaaaactatt agatgatgct ggctacaaag
    59881 atgtggatga cgatggttta agagaagaca aagatggaaa taaactaaca atcaactttg
    59941 cttcgatgtc cggtggggaa acagcgcaac cattggcaga ttactatatc caacaatgga
    60001 aagaaattgg tttggatgtc caatacacaa caggtcgtct gatcgaattc aattctttct
    60061 atgataaatt agaaaacgat gatcctgaaa ttgatgttta ccaagctgct tggaatacag
    60121 gaactgatcc aaatccaagt ggactatggg gaccaaatgc tgcgttcaac tacactcgtt
    60181 atgaatcaga agaaaataca aaattgatca acgatatcag ttcaagcaaa gcatttgatg
    60241 aagagtacca aaaagaagca tatcaaaaat ggcaacaata tgcatttgaa gaagcatttg
    60301 cgattccaac gttgttccgt aatgaagtat tgccagtcaa cgaccgtgtc aaagactggg
    60361 attggtctta tgatgtacca aacccatggg caaatgtttc tgttacagca gattcacgtt
    60421 cataatcact aaaaatgacc gaaaaagggc gatgtccttt ttcggttttt tgatattttt
    60481 ctgtttattt ctctaatatt caggtcaagg cgctgatttc atgacttctc caataaacgg
    60541 tgttcaaagt ccagtttctc ggtattaagt caaatttatg caaaaatatg gaaagccatt
    60601 ttcacaaaat tttttcttca tactcgaaac taaacgaatt tttcacaact tctcgtcaat
    60661 cactaggatt attaaagctt tacttacctt ttgaccgaac catttcaaat ttgacaaaaa
    60721 accagtataa taaggaagta tcgaaaaaat atccataaaa ggaatgtgga aaatggacaa
    60781 ccagctgaca aaagaactaa aagaaaacta taacatcgtt tttcacgatc taaatttgct
    60841 tgaacaagct tttacccatt catcctatgt gaatgagcat cgcaatctgc aattatcgga
    60901 taatgagcga cttgaatttt taggagatgc cgttttagaa ctgatggttt ctcagtattt
    60961 attccgttta tatcctcatt tgccagaagg aaaattgaca aaaacacgag ctgcgatcgt
    61021 tcgcgaagac agcttgtcga aatttgccaa agaatgtcac tttgatcaat atgttttgtt
    61081 gggcaaggga gaagagaatt caggaggacg tacgcgacca gcattgcttt gcgacctatt
    61141 cgaagctttt ttaggcgcgt tatatcttga ccagggattc gacacgaccc atgcattcat
    61201 cgaaaaagtg atttttccta aagtcaaagc aggtgctttt tcacatgaga tggatcataa
    61261 aacaaaacta caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat accgattgat
    61321 caatgaagaa ggtccagctc atgaacgagt attttggatc gaagtttatg tagatgatca
    61381 attgattggc actggacaag ggaaatcgaa aaaattagca gaacaagctg cggcggaaaa
    61441 tgcattggca gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa cgaattgaaa
    61501 tagccggttt taaatcattt gcagacaaga cagtcattga ttttgaaaat agcgtcacag
    61561 cagtggttgg accaaatggc agcggaaaaa gcaatattac agaggctatc cgctgggtat
    61621 taggagaaca atctgctaaa agtctgcgag gcggaaaaat gccggatatc atctttgccg
    61681 gttccgatac ccgtaagcag ctgaatattg cagaagtcac agtgatttta gacaatagtg
    61741 atcattatct gccattggag tacaatgaga tcagtgtaac ccgccgttat cgacggactg
    61801 gggaaagcga gtttttcatc aataaacaaa gctgcagatt aaaagatatc caagaattgt
    61861 tcttagattc tggattagga aaagaatcgt tctcaatcat ttctcaagga aaagtagaag
    61921 cgattttcag tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca gctggtgtct
    61981 tgaaatacaa gcagcggaaa aagaaagcag aacagaagct ttttgaaaca gaagacaacc
    62041 tcagccgcgt ccaagacatc atccacgaat tggaagaaca gttaactcct ttggccgccc
    62101 agagtgaggc agcaaaggag tttttacggc taaaagaaac actgactcaa accgatgtct
    62161 ctttgatggt tgctgaaatc aagacagcaa aaaaagattg ggacaacaaa caagcacagc
    62221 ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc gatccaagaa caagaatcca
    62281 ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa aaaaatcagc
    62341 aagtattatt agatctttcg gaaaaactga agcagacaga aggacaaaaa gatgttttgc
    62401 aggagcggac aaagcataca caaaaaagct cgcaagaata tcaaacttca ttagcagaag
    62461 cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag cttgatgaaa gcagcagcag
    62521 aaaaagaaac agagatccaa aaagctgaag caaatctcat aaaaacacag caagaattag
    62581 aaaaatacca aaaatcaacg aaagaattat tggcagaatt aagagatcaa tatgttgatt
    62641 tgatgcaaga acaagcagct gtaggaaacg aactgaagta tctagaacgt caatatatac
    62701 aagaaacagc aaaaagcaag cagacgttag ccaagcaatc ggaagtagaa gcatcagtag
    62761 accgattgat cttacaaaaa gaagaattga cacaaaagca ggcgcagcta aaatcatctt
    62821 taactgaaac aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag ttccaagaag
    62881 cattagcaaa agaacagcct aaaatgtatc aattaatgaa tcaagtccag cagctacgtg
    62941 ctcgtaagaa aagccttcaa gaaatccaag aaaactattt tggtttttac caaggggttc
    63001 gattggtctt gcagcataaa cagcagcttt cgggtattgt cggagcagtg gctgaactga
    63061 tcgatgttcc tgcagatttc acgttagcta tcgaaacagc tttaggtggg gcagcccagc
    63121 atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg aaacagcaaa
    63181 gaggaggaag agcaacattc cttccactga cgacaatcaa accaagacaa ttaccggctc
    63241 atatattgac acaagcagca gctgtagaag ggtttatagg gattgccagt gaacaagtca
    63301 gttatcccga tcaaatccaa actgtggttc aaaacctgct aggaacgatc cttttagcca
    63361 aagacttaac tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat cgagtggttt
    63421 ccttagaagg tgatgtgatg aatgccggag gatccatgac aggtggagcg aataaacgag
    63481 gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa acagttgact agtgaatttg
    63541 aagaagcaga taaacaactc caagcacaag aaaaaaaagt tcaggagctg caacaagaaa
    63601 cagctcgttt agccgaagaa caagaagttt tgcgtacacg aggcgaacaa ttacgttttg
    63661 aagaacaaga agcgacaaat cagctacaaa acatcataaa tgaacttgaa cgttttgaaa
    63721 aagaaaagca aatctcaact tttgagacac gtgaactgca gcagtttatc gaagattacg
    63781 aaaagcaaac gaacgaattg aaagacaaac aaacagatct tgaatcgcaa cgacaaaaaa
    63841 tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc agaagagcac
    63901 aagtacaaag tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa gagcagttta
    63961 atcatctgca gatccagctc cgcggtgccc gagtccaaaa agcagaggca accgaacgtc
    64021 aagaagcgat agaaaagcag ttggcgacat tgacagcaga cttttctgat catgaagtca
    64081 cagaagaaag tttggaaaaa caaattaacg aattatctgc tcagcgagaa acattaaaag
    64141 ccgagttggc aaaagccaaa gagcagcgag atagaaggca aaaggagatc gataagcttg
    64201 aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa caatccaaat
    64261 tagaagtcca aaaagaccga gctgaaatga ttttagataa tcatttgacc tatctgcagt
    64321 cagaatatca gctgacattt gagaaagctt cccaagacta tcaagaaaca accgatatcg
    64381 aagacagtag aacaaaggtt tcatccttaa aagaacagat tgaaaaactg ggaccagtaa
    64441 acttaaattc aatcgaacaa tacgaacaag tcagtgaaag acatacattc ttagcaacac
    64501 agcgagatga tctgttagca gcgaaaaacc aattgtttga aacgatggat gagatggatg
    64561 atgaagtgcg gacaagattc aaagaagtat ttgaagctat ccgtcaagaa ttcaaaattg
    64621 tctttccaaa tatgtttggc ggcggtaaag ctgaattagt actgacggaa ccaactgatt
    64681 tgctgaaaac aggtattgag attgaagtac aacctcctgg caaaaaactg caaagtctaa
    64741 gtttgctttc tggaggagaa cgagcactta cagccatcgc gttgctcttt tccattatcc
    64801 gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga agcagcgtta gatgaagcca
    64861 acgtgaaacg ttttggtcgt tacttgagcg atttccaaga tgacacacaa tttatcgtag
    64921 tcacacaccg caaaggaacg atggtcgcag cagatgtact gtacggagtg acgatgcagg
    64981 aatccggtgt atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa gaggcgaaaa
    65041 tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg atttggacgg
    65101 tacattatta aatgaacaaa agctgatttc agatgagaat aaacaagcat tagcacaagc
    65161 aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc cccttagctg cgatggcgca
    65221 ttatctgcaa gaattaggac tggtagatga aggtgactac agcatcactt tcaacggcgg
    65281 tttggtacaa aaaaatgata ctggtgaaat catggaaaag aaagtgatgg aagtatcaga
    65341 tatccaacgc ttatacaaac tggctcagca attggatctg ccactagatg ttttgtctga
    65401 caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca atgtgttgaa
    65461 taagctgctg caattccaac cagcggatat aagcgatatt acagatgaat ttgtgcttaa
    65521 taaggcagta atcgcttatg accaagaaga attagatcct aaaatcaaag aaataccagc
    65581 agaattccat gaaatgtatg agatcatcaa aacaagaagt atgcttttgg aatttatgcc
    65641 aaaaggtgtt acaaaagctt atggtatttc tttattggca aaagatcttg gtttgaaaca
    65701 agaagaaatc atggcgatcg gtgatgagga aaatgattta ccgatgatcc aatatgctgg
    65761 actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag atttcgtgac
    65821 agccagcaat gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat tagagtaggg
    65881 ggagtcaaat gggatttttt gataaaatca agcgtgcttt tactggcgaa gagaaaacag
    65941 aagaagttga agtacaggag aagtacgaca agggtttaga aaagacgcgt aaaacatttg
    66001 gtgaacggat gaatgagttg tttgccaatt ttcgaacagt cgatgaagac ttttttgaag
    66061 aattagaaga aacacttatc ggtgcagatg taggttttga aacagcgata cgaattaccg
    66121 aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg
    66181 cgatcatcga gaaacttgtg gatctctatg aagcggaagg aatcaatgaa gtaaatgaac
    66241 taaatatcca agaagacgga ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga
    66301 aaacaacaag tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga
    66361 tggcagcagc agataccttc cgcgctggag caatcgacca gttagttgtc tggggcgaac
    66421 gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga tccagcagct gtcgtattcg
    66481 atgctttgga aagagcaaag acagagcagg cagatgtttt actagttgat actgcaggcc
    66541 gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa aataaaacga gtaatccagc
    66601 gggaattacc agaagcaccc caagaaacac ttcttgtatt agatgcaacc actggtcaaa
    66661 acgcgatggt tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt cttgttttga
    66721 caaaattaga tggtacagca aagggcggta tcgtacttgc tatccgtaat gaattgcatt
    66781 taccagtgaa attggtcggt cttggtgaag gaatcgatga cttggagccg tttgatccaa
    66841 atgattttgt cgttggattg ttcaaaggat tactaaaaga agaataaccc atgtctaaaa
    66901 agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt aacatagctg
    66961 tgtttttgat gccttcattt ttatacttat ttgtaagcgg ttctcaaaca atttcgttct
    67021 ttttcttaga ctttttgatt ataattagat tcatatgaat tggaggtcac ttaatgatga
    67081 attttaagca attattattg catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg
    67141 gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc
    67201 cagcaatctt tggcgatcga tcctatcacc cctatatcca aacagatttt agcgaaacac
    67261 aaacagagat gatcacccct gttaccgatt ctattcccga attatttcag tatctcgctg
    67321 ctgtttatga tgtgactgct cgttctatac cgaaagaaga gatgatctgg ccattaagta
    67381 tgccacctgc cttaccggaa aaagacgaag agatcattat tgcaaaatta aaaaatttcg
    67441 aagatgtctt gtatcgacgt tatttagcaa aagaatacgg gaagcgaaaa cagatggtaa
    67501 gcggtattca tttcaatttt gaatttggcg atgagttgct aagaacattg ttcagccatc
    67561 aggaagaatt tcaagatttt tccgaattca aaacagaact ttatttgaaa acagcgagaa
    67621 actttatgcg ctatcgctgg atgatcactt atttattcgg tgcttcacca atgagtgaga
    67681 agaactactt tttagatgaa tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg
    67741 cgctgggcta tacgaatcat ccaaatgtga aagtttccta tgcttctatg aaacagtatt
    67801 tagcagatat cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa gaattttata
    67861 caccgcttcg tttccgaggc ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt
    67921 atattgaatt gcggaatatc gatttgaatc cttatgcaag attgggtatc aatccagagc
    67981 aagttcggtt cttacaatta ttcctgatgt acatgttatg gacagaagaa aaagaagact
    68041 gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa agtagcacta gaacaaccat
    68101 cagatcaaac agaatttcat caagaaggta gagaaattct tgaaggaatg aagcaaatgc
    68161 tggttgaatt ggattggttg gattctcttt atttagtaga ggaagcattg actcaaatgg
    68221 atcatcctga acaaacgtta gcagcgaaac tctatcaaga agcacagcta tcgagtcagc
    68281 aagaagttgc tgtggcattg ggacatcaat attataaaga aagccatgaa cgtccctatc
    68341 agttggccgg ttttcgcgaa atggaacttt ctactcagat atttatgttc gatgccatcc
    68401 aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca atttttgcgt ctgcaattcc
    68461 aagaccatgt agaatatgtg aaaaatgcaa acatgacaag taaggacagc tatatcgtcc
    68521 cacttatcat ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct gggttccggg
    68581 taccaggagg cgcagaattt tcatccatgg aagaagcggt aaaagcttat ccgagatttg
    68641 ccgagcaggc atttgtgatc aaacctaaat caaccaatta cgggttaggt atcacgattt
    68701 tcaaggaagg agctagtctg gaagattatc aagcaggttt agccattgct tttcgtgaag
    68761 acagttcggt tttagtagaa gaatttatgc cgggaacgga atatcgtttc tttgtgatcg
    68821 acggagaagt ccaagccatc atgttgcgag tccctgctaa tgttattggt gacagtatcc
    68881 gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc
    68941 gtgcaccttt agaattgatc caattaggtg aattagaaca attgatgttg aaagaacaag
    69001 ggttgacgat cgaatcagtt ccccaagcca atcaaattgt ttatctaagg gagaactcaa
    69061 atatcagtac aggtggtgat tcgatcgata tgactgatga attttctgaa gcttacaaaa
    69121 aaatcgccgt ttctgcggta gaagcactag gagctaagat tagtggtatc gatttgatta
    69181 taccagataa agaaatcgat cctacaactg ataaaaaagc atatgggatc atcgaagcaa
    69241 atttcaatcc agccatgcat atgcatgttt atccttttgc aggaaaaggc agaagattga
    69301 cgatgaatgt attgaaattg ttatatcctg aagtatttta aaagaaaaag cttttgacag
    69361 attgtcctat ttgatgtcaa tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt
    69421 tttttcgttt taaaataaac gatcccagaa gctttttttc ttttttggtt tttctttttc
    69481 agctggcgaa gagttttctt ctgggtattt tttttcgata tcaatcacag gttcatttac
    69541 tgcttctttt gctgttaaga gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt
    69601 gactacagtg aaacggctat tgtttttcgt tgctagctgg atatattggt tttggacaga
    69661 aaggggtaga gccccattca agagtatgga agcatctgga tgttttttga gttcttttaa
    69721 aaagttttct ttgttttttg gttggatcat ttcagcaaca gtcatactaa gatagcagcg
    69781 ttccctgaat gtccctaaat atttatgctg ttcttcaggg ttcactaaag gtgtaccata
    69841 aagtccctta tccaaatggc tttttacatc atcttgtgcc ataaaaaata ccctccttct
    69901 tctctttgtc tttcttacga ttatagcata ttcccaataa tacctaaaac atatgaaaaa
    69961 gcggttgctt ttgttcaacg attcacttaa aataagaatg aaaatcattc tcaattagaa
    70021 aggtggattg gatgcgctac ggacgactag tagcattatt agttatttta agtagtattt
    70081 cattgttcat cggagtatcg aatgtacaat tgtcagaact gctccagtta gatgggcaaa
    70141 gcttacaaat cttgctagtg actcgtttgc cacgaacgat ttgtttgatc cttgtgggag
    70201 cgaccagtag tatttgtggg ttgatcatgc aacatttgac ccaaaataaa tttgtttcac
    70261 caacgactgc gggcacgatg gatagtgcgc gtttaggtat tttagtggct atgattttcc
    70321 tacctggtgc atccttgatt gtccgatcct tgacagcatt ttgctttgct tttgcaggaa
    70381 cattgctttt tttatccctt acgcgattgt tcctccagaa aaatcaattg atgcttccgt
    70441 tgattggagt catgttaggg aatatcattg gttcagttgc tacttttttt gcttatcaat
    70501 tccagttggt acaaaatatg acttcatggc ttcaagggaa ttttgcgact gtgatacgag
    70561 gaaattatga gttattgtat gctgttgttc cattgttgct cttggtttgg ctatttgctt
    70621 accgatttac gatcattggt ctgggcgaat cgtttgcagt aaatctggga atagattacc
    70681 agaagatgca gtttttaggc attggcttag tatcattggc tagtgcagtc atgttgatca
    70741 tggtaggaag cattcctttt atcggagtga tcattccgaa tcttgtctct agaatatatg
    70801 gagatcaagt gcacaaaaca attggcgtaa ctgctttagc aggtagtttg ttcttagtat
    70861 tttgcgatct tgtcgctcgc gtgttgattt ttccttatga aataccagtc agtgtgatcg
    70921 ttggaatcat cggtggggct atcttccttt atcttctgat aaggggacga cgatatgctt
    70981 aaaaaaaagc catctatttt tattggtatg cttgttcttg ttttatttgt tacaggaatg
    71041 tacttgacga tccatacata tggaaactgg cattttgcat gtgcattgag gagtaagaaa
    71101 atttttgcat ttatccttgt ctcgctagct gtcagcagtg ccacaatcag ctttcagaca
    71161 attacgcata accagttttt gactccttca gtattgggac ttgagtcgtt atatgtcctt
    71221 gtccaaacgg tccttttctt ctttatcgga ggagtgacga tgcttcagca gacaggtaca
    71281 gtttttttct tattgaacgt tgggctgatg gtcgggctga gtctattgtt atttttcttt
    71341 cttttgaaaa aaatgagagc gaatcttttt ttattactga tgtcagggat gattttaggt
    71401 acgctttttg gtagtgtcag taccttttta caagtcatca tggatccgaa tgaatatgat
    71461 ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc agagtcagta tcttttgcct
    71521 gcttctttga tcattatcgg ctgctggtac ctgttatttc gatatcgaca tgtgctagac
    71581 gttttccatc tcggaaatga acaagcagta aacctcggga tagacacaga tcgttttgaa
    71641 tttttgattt tatgttgtgt cagtcttttg acagggacat caacggctct tgtgggtcca
    71701 gttacttttt tagggtttat tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt
    71761 catggtcagc tatttatcgg aagctttctg gtagctatgc tgtttctgac aggaggacag
    71821 ctcgcagtag agcagatttt cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc
    71881 ggaggaattt attttgtcac aaaattgatt cgcgaaagga agagtatcct atgattgatt
    71941 tacaaaaggt caataaactg taccatggaa aatcagtggt taaagatgta cagttaacga
    72001 ttcctgaaaa gaaattgact gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt
    72061 tgtcgatgat cagtcgtttg ataccaaaag atacaggtga aatctattta gaccataacg
    72121 aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt atcgatccta cgacaatcga
    72181 atgaaataaa tgtccaaatc accgtgaaag aattagtaga atttggacga tttccttata
    72241 cgaaaggaaa attgacgaca aaagacgagg aaatcgttca ggaatcactg gaacaccttg
    72301 ggttagaaga aatagcggat caatcgattg acactctttc aggcggtcaa ctccaacgtg
    72361 cgtatattgc aatggtgcta gcgcaagaca cggattatat tttactggat gaacctttga
    72421 acaatctaga tatgaatttt gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag
    72481 aaatcgggaa aacaattgtt attgttcttc atgatatcaa ctttgctgca agttatgcag
    72541 acgaaattgt tgcaatgaaa gatggacagt tatttacgca aggaacgacc caagaaatga
    72601 taaaaaaaga tgtattggat gaattatacg aaatggacat acggatttgt gagattgaag
    72661 gccgcagatt ctgtctttat ttctaaaact aacattagga gagaagagat gaaaaaatta
    72721 gtagtaggta tcttgattct agcatcattt ggtttagcag catgtggaaa tagcaacaac
    72781 accagtcagg cagacacaaa aaaaagcagc acacaaactg aaactacatt gacgataaca
    72841 gatagtaatg gagaccaaat cgaggtgcca aataatccag aaaaagtcgt cgtattcgac
    72901 aatggttctt tagatacgat ggatgctctt ggtgttggag ataaggtagt tggagcagct
    72961 acaagcagcc ttcctgagta tctttcatcc tataaaaaag tagaatcagc aggtggaata
    73021 aaagaaccag acttagaaaa aattaatcag cttcagccag atctaatcat catttctggc
    73081 cgacaaagag attttcaaaa agatctaagt caaattgctc cgaccatttt cctttcttta
    73141 gatgcgaaaa atccatggga atctttccag caaaatgtca ctgctttagg ggagatcttt
    73201 ggtaagcaag aagaagctaa aactcaatta gaggaattat ctagtgcaat cgatcagacg
    73261 aaaaagaaag cagaagctac tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa
    73321 ttatcagcct acggatctgg ttctagattc ggtttcatcc atgatttatt tggctttgaa
    73381 caagcagatg atcaaatcga agcatccact cacggtcaaa gtgtttctta tgaatatgtg
    73441 ctggaaaaaa atccggatat cttatttgta gtagacagaa caaaagctat tggtggagac
    73501 gacagcaaag atgatatttc tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat
    73561 cagcagatca tctcccttga accagatgtc tggtatttaa gcgggggcgg tttagaatca
    73621 atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat agcataaata acagaggatt
    73681 aaactctcat actatgtaac aataatgttt cttacattct taaatagcaa gagcctatcc
    73741 tcaaagaagt tatcaaaaac catccgtaaa atcgtattga tgattttacg gatggttttt
    73801 atttatttga gaaatctttt ttcaacgtga attcattatc tacatcatac gggttgattc
    73861 tagaatgggg agtggcaaaa aatagtgtga tccatttttt cagcatcaag atcgcctcct
    73921 ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa cttaaattta gatattttta
    73981 ttttgtaccg atctcttctt taaacgatgt tccatctgct gcttctcggc aataagtcca
    74041 ctagactaga aaatatgaga agctattttc tgtccagcgt tcttattact caaagcaaat
    74101 cgcagttgtc acaatctctt ctaaacgatg ttccatctgc tgcttctcgg caataagccc
    74161 actagactag aaaatatgag aagctatttt ctgtccagcg ttcttattac tcaaagcaaa
    74221 tcgcagttgt cacaatctct tctaaacaat gttcaatcag ctgaccttcg gtattagctc
    74281 aaagcactga tttcacaacc tctatttagg catgtgtctc agcaagcggt tgatagttga
    74341 tgatctgcat ttttcgataa aaatcggcgg gaagcttact ccaccgtttt aataattgat
    74401 agaaataact ctcttctgct ttgattttat actcgcttaa tatcaatcca aaactattcg
    74461 ttttttttga agctaatttt ttataaatat ccaatgcttt ggttttatct tcattgacaa
    74521 agagtgtttt tacttcaata taagaaccct cgatattttg tgtttcttcg ataaaataga
    74581 tgttttttat atattccttc atagtgattc ctccaaagaa gtcttttatt tgataagttt
    74641 actctaccga agagttgtta ataatccgtg aacaaaaagt aattcttttt ccaatagttg
    74701 ttaaaggttt tttatttttt atagtatttt ttgttattta agaaaggaat aaggttaaac
    74761 aaataacagg ttgctttttg ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa
    74821 agttgtctct tttgtgataa gagaaatcat aataaacgaa tgacttactt tccaataaat
    74881 tgagaacagt cagaaccaga caaattagtt gcatctgatg aataaagtga ttattctatg
    74941 acatgaggtg atacagatga aattagaagt gacaaaagaa gcacaagaag tattgaagaa
    75001 taaattagaa gcagacgatc aacttttatt agatatcgaa aacggtgacg gtccatttgc
    75061 agaaagccaa gtcagttgcc aattggatac ggcatttcga ttgatcattg tcaaaaaaga
    75121 tacacaaaca gatctaagtc tttattctgt agtggctgat acaccagttg gaccaatcaa
    75181 aatcaaagac agtgcaatac tttacatgga tgatccatcc acacttgctt tagaaccaac
    75241 ttataatagt ttacaattaa aaggaccaag cggcttgaga aaaggcaact tgcaagttgt
    75301 tcgaaaagac tagagggata aaatgggaaa attggcgatc atcagcgacc tacatgtgga
    75361 catcaataaa ttcggcgaat cagagctgat gcagctttgg caagttttgc aaaagcacaa
    75421 gatcactcgg cttcatctag ccggagatac ggctaataaa gtagaccgtt gcctagctgt
    75481 tgtcgaattt ttcgtcaata aaggattatc tacgacattt catttcggca atcacgagtt
    75541 ggcagatgtg cccaatgaac agatgatgga ccattttcca gacccacatt ttttaaatga
    75601 acggtatatt gctttaaatg agcagaccgt tcttttaggg atgaatggct ggtacgacta
    75661 tcagttttcc gaattgaaag attacgaggc aatcgtacga atgaagcgtt tgtattggta
    75721 tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc
    75781 tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt gaggtgattt tatccactca
    75841 tttcgttcct aaacgggcat ttatcatgta tcaatctgct ccttatgagc gttggaacaa
    75901 attaaatgca tttttaggat cagaatcatt cggagaatta cttgaccgtt attcaaatat
    75961 caaacaagta gtttttggcc atacgcatcg tcgctttgaa gataaatcca tcggtcacac
    76021 gatttacagc tgcagacctt ttggttatta ttatgaatgg cagctgaccc gagattttgt
    76081 cttgaaagaa cagttgctag acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc
    76141 ccatcaccca gcttttcagg cctatcaaac acaacatttg caagaggaat ttgaacaagc
    76201 gatgacaatc gttccgtatt gagacagcat tagtaatgtg taaaagaaaa taccttgtaa
    76261 aacataagga taagtaagag cagtagatcc atttatcgtg gatatgctgc tttttttgcg
    76321 gaaaataatc agcgaatgtt tatttgatcg gatgatttgt aactcgcaga gtactagatg
    76381 caagcgtgta ttcttttttt gaacgataat cacaaaaaaa aatata
//
LOCUS       DACDBG010000004        70961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDBG010000004 DACDBG010000000
VERSION     DACDBG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.004"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IVZ90_000238"
     CDS             162..392
                     /locus_tag="IVZ90_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8261317.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IVZ90_000239"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IVZ90_000239"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8261318.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IVZ90_000240"
     CDS             1803..1979
                     /locus_tag="IVZ90_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8261319.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IVZ90_000241"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IVZ90_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8261320.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IVZ90_000242"
     CDS             2474..2941
                     /locus_tag="IVZ90_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261321.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IVZ90_000243"
     CDS             3026..3910
                     /locus_tag="IVZ90_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8261322.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IVZ90_000244"
     CDS             4027..4260
                     /locus_tag="IVZ90_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8261323.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IVZ90_000245"
     tRNA            4348..4428
                     /locus_tag="IVZ90_000245"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IVZ90_000246"
     tRNA            4435..4506
                     /locus_tag="IVZ90_000246"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IVZ90_000247"
     CDS             5021..5728
                     /locus_tag="IVZ90_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8261324.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IVZ90_000248"
     CDS             complement(5788..6432)
                     /locus_tag="IVZ90_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8261325.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IVZ90_000249"
     CDS             6559..6846
                     /locus_tag="IVZ90_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261326.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IVZ90_000250"
     CDS             6954..7274
                     /locus_tag="IVZ90_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8261327.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IVZ90_000251"
     CDS             7355..8668
                     /locus_tag="IVZ90_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8261328.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IVZ90_000252"
     CDS             8665..9384
                     /locus_tag="IVZ90_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8261329.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IVZ90_000253"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IVZ90_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8261330.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IVZ90_000254"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IVZ90_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8261331.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IVZ90_000255"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IVZ90_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8261332.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IVZ90_000256"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IVZ90_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8261333.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IVZ90_000257"
     CDS             14821..15714
                     /locus_tag="IVZ90_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261334.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IVZ90_000258"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IVZ90_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8261335.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IVZ90_000259"
     CDS             17651..18508
                     /locus_tag="IVZ90_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8261336.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IVZ90_000260"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IVZ90_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8261337.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IVZ90_000261"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IVZ90_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8261338.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IVZ90_000262"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IVZ90_000262"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8261339.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IVZ90_000263"
     CDS             21264..22022
                     /locus_tag="IVZ90_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8261340.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IVZ90_000264"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IVZ90_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8261341.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IVZ90_000265"
     CDS             22950..23981
                     /locus_tag="IVZ90_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8261342.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IVZ90_000266"
     CDS             23978..24745
                     /locus_tag="IVZ90_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8261343.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IVZ90_000267"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IVZ90_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8261344.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IVZ90_000268"
     CDS             26713..27030
                     /locus_tag="IVZ90_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8261345.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IVZ90_000269"
     CDS             27030..27380
                     /locus_tag="IVZ90_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8261346.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IVZ90_000270"
     CDS             27547..28485
                     /locus_tag="IVZ90_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8261347.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IVZ90_000271"
     CDS             28500..29330
                     /locus_tag="IVZ90_000271"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8261348.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IVZ90_000272"
     CDS             29374..30399
                     /locus_tag="IVZ90_000272"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8261349.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IVZ90_000273"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IVZ90_000273"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8261350.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IVZ90_000274"
     CDS             31477..31917
                     /locus_tag="IVZ90_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8261351.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IVZ90_000275"
     CDS             31920..32522
                     /locus_tag="IVZ90_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8261352.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32581..33687)
                     /locus_tag="IVZ90_000276"
     CDS             complement(32581..33687)
                     /locus_tag="IVZ90_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8261353.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IVZ90_000277"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IVZ90_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8261354.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35235..37580)
                     /locus_tag="IVZ90_000278"
     CDS             complement(35235..37580)
                     /locus_tag="IVZ90_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8261355.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37859..38761
                     /locus_tag="IVZ90_000279"
     CDS             37859..38761
                     /locus_tag="IVZ90_000279"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8261356.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38974..40749
                     /locus_tag="IVZ90_000280"
     CDS             38974..40749
                     /locus_tag="IVZ90_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8261357.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40815..42401)
                     /locus_tag="IVZ90_000281"
     CDS             complement(40815..42401)
                     /locus_tag="IVZ90_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8261358.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42652..42876
                     /locus_tag="IVZ90_000282"
     CDS             42652..42876
                     /locus_tag="IVZ90_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8261359.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43018..44769
                     /locus_tag="IVZ90_000283"
     CDS             43018..44769
                     /locus_tag="IVZ90_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261360.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44769..46553
                     /locus_tag="IVZ90_000284"
     CDS             44769..46553
                     /locus_tag="IVZ90_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261361.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46879..47730
                     /locus_tag="IVZ90_000285"
     CDS             46879..47730
                     /locus_tag="IVZ90_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261362.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47784..49535
                     /locus_tag="IVZ90_000286"
     CDS             47784..49535
                     /locus_tag="IVZ90_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261363.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49539..49709
                     /locus_tag="IVZ90_000287"
     CDS             49539..49709
                     /locus_tag="IVZ90_000287"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261364.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49749..50651)
                     /locus_tag="IVZ90_000288"
     CDS             complement(49749..50651)
                     /locus_tag="IVZ90_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8261365.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50882..52285
                     /locus_tag="IVZ90_000289"
     CDS             50882..52285
                     /locus_tag="IVZ90_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8261366.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52285..53718
                     /gene="ascB"
                     /locus_tag="IVZ90_000290"
     CDS             52285..53718
                     /gene="ascB"
                     /locus_tag="IVZ90_000290"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8261367.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53863..54789
                     /gene="trxB"
                     /locus_tag="IVZ90_000291"
     CDS             53863..54789
                     /gene="trxB"
                     /locus_tag="IVZ90_000291"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8261368.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55003..56730
                     /locus_tag="IVZ90_000292"
     CDS             55003..56730
                     /locus_tag="IVZ90_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8261369.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56802..57095
                     /locus_tag="IVZ90_000293"
     CDS             56802..57095
                     /locus_tag="IVZ90_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8261370.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57187..58047)
                     /locus_tag="IVZ90_000294"
     CDS             complement(57187..58047)
                     /locus_tag="IVZ90_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8261371.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58139..58567
                     /locus_tag="IVZ90_000295"
     CDS             58139..58567
                     /locus_tag="IVZ90_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8261372.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58729..58905
                     /locus_tag="IVZ90_000296"
     CDS             58729..58905
                     /locus_tag="IVZ90_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8261373.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58932..59378
                     /locus_tag="IVZ90_000297"
     CDS             58932..59378
                     /locus_tag="IVZ90_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8261374.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IVZ90_000298"
     CDS             complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IVZ90_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8261375.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61617..62588
                     /locus_tag="IVZ90_000299"
     CDS             61617..62588
                     /locus_tag="IVZ90_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8261376.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62612..64804
                     /locus_tag="IVZ90_000300"
     CDS             62612..64804
                     /locus_tag="IVZ90_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8261377.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64821..65297
                     /gene="ybeY"
                     /locus_tag="IVZ90_000301"
     CDS             64821..65297
                     /gene="ybeY"
                     /locus_tag="IVZ90_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8261378.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65275..65676
                     /locus_tag="IVZ90_000302"
     CDS             65275..65676
                     /locus_tag="IVZ90_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8261379.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65691..66590
                     /locus_tag="IVZ90_000303"
     CDS             65691..66590
                     /locus_tag="IVZ90_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8261380.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66733..67545
                     /gene="recO"
                     /locus_tag="IVZ90_000304"
     CDS             66733..67545
                     /gene="recO"
                     /locus_tag="IVZ90_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8261381.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            67929..68846
                     /gene="glyQ"
                     /locus_tag="IVZ90_000305"
     CDS             67929..68846
                     /gene="glyQ"
                     /locus_tag="IVZ90_000305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8261382.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68848..70923
                     /locus_tag="IVZ90_000306"
     CDS             68848..70923
                     /locus_tag="IVZ90_000306"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8261383.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23221 a        12429 c        15180 g        20131 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctgggaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32581 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32641 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32761 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    32941 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33001 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33181 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33301 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33361 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33421 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33481 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33541 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34081 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34141 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34201 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34261 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34501 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34561 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34681 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34741 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34801 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    34921 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    34981 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35041 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35101 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35161 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35221 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35281 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35341 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35401 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35461 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35521 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35581 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35641 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35701 acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35761 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35821 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    35881 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    35941 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36001 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36061 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36121 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36181 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36241 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36301 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36361 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36421 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36481 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36541 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36601 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36661 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36721 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36781 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36841 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    36901 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    36961 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37021 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37081 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37141 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37201 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37261 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37321 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37381 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37441 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37501 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37561 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37621 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37681 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37741 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37801 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37861 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    37921 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    37981 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38041 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38101 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38161 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38221 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38281 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38341 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38401 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38461 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38521 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38581 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38641 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38701 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38761 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38821 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    38881 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    38941 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39001 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39061 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39121 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39181 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39241 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39301 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39361 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39421 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39481 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39541 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39601 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39661 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39721 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39781 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39841 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    39901 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    39961 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40021 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40081 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40141 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40201 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40261 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40321 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40381 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40441 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40501 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40561 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40621 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40681 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40741 tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt
    40801 ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt
    40861 ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg
    40921 attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag
    40981 aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag
    41041 aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg
    41101 ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc
    41161 tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt
    41221 gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt
    41281 agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta
    41341 ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag
    41401 ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa
    41461 gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca
    41521 cccatgatca atagcagtgc attgactaga aaagccattg acaactggat attagaatcc
    41581 caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga
    41641 gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc
    41701 cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga
    41761 ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga
    41821 tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga
    41881 aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca
    41941 atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca
    42001 atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta
    42061 cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct
    42121 gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa
    42181 tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca
    42241 actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc
    42301 tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata
    42361 agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta
    42421 tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac
    42481 gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta
    42541 aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt
    42601 tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca
    42661 acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt
    42721 ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac
    42781 atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag
    42841 atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg
    42901 ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag
    42961 caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
    43021 tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
    43081 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
    43141 atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc
    43201 ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt
    43261 tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca
    43321 gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg
    43381 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
    43441 gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca
    43501 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa
    43561 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
    43621 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
    43681 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac
    43741 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
    43801 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
    43861 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
    43921 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc
    43981 gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc
    44041 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa
    44101 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa
    44161 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
    44221 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
    44281 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
    44341 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
    44401 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
    44461 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc
    44521 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
    44581 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
    44641 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
    44701 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
    44761 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
    44821 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg
    44881 ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc
    44941 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
    45001 ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt
    45061 atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
    45121 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
    45181 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
    45241 ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg
    45301 ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat
    45361 tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga
    45421 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
    45481 aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga
    45541 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt
    45601 tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
    45661 ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
    45721 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
    45781 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
    45841 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
    45901 ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca
    45961 tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
    46021 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
    46081 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
    46141 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
    46201 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
    46261 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
    46321 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc
    46381 gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
    46441 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
    46501 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt
    46561 tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt
    46621 tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt
    46681 cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata
    46741 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
    46801 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat
    46861 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc
    46921 agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa
    46981 gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg
    47041 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
    47101 tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat
    47161 attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta
    47221 ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa
    47281 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    47341 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
    47401 ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    47461 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    47521 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    47581 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    47641 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    47701 cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat
    47761 gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag
    47821 aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa
    47881 atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa
    47941 aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag
    48001 ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag
    48061 caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc
    48121 ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga
    48181 tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca
    48241 catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg
    48301 gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag
    48361 cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa
    48421 cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc
    48481 cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag
    48541 ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg
    48601 ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat
    48661 tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag
    48721 ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt
    48781 gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga
    48841 cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc
    48901 ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga
    48961 ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct
    49021 gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc
    49081 aaggtggact aggcatcttt acaattccca actatattag accagatggt gatttaacag
    49141 atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt
    49201 ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag
    49261 aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag
    49321 cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac
    49381 gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg
    49441 tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag
    49501 ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc
    49561 tacgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtgatcg
    49621 tcaccaatta cacggattat ttggatattt tggaaaacca aggggagacg gtcgggccag
    49681 acgatgcact tatcaccgca ctgacctgaa cggttaggtg aagaatggac taggcttaac
    49741 tattcttttt attccacttc tttattttca aaattgaaag gctctttcat gatggctgag
    49801 ctgatagggc gtttatcgaa tttttgaccg acagaaatta gatggactaa attctgttga
    49861 taattttctg caaaaacaca actatataac acgtctagaa gataacaaat tgatgtattg
    49921 atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct
    49981 gtactcattg cagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga
    50041 ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc
    50101 agaatcccgc acatatcact cgtgcaattg tacgcttcat agaatgattc accaaaagtg
    50161 gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc
    50221 aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg
    50281 gctttttgca gttggtcatg atgaatgaga gaaagtgtat catgaatcgt catctgttct
    50341 aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc
    50401 gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga
    50461 aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt agaggggtgg
    50521 acataacatc gctcagcaat cgcttggatg gtcatttcgc caagtgctgg agaatcgctt
    50581 aagagaaact ggatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca
    50641 cgtaataaca tgtagatacc ttcttcttat ttttttacgt aaattataac ttttttgaaa
    50701 atgatttgta aacggattta tactatataa cttaaaatag acattttttg acaagatatt
    50761 tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga aacagatttc
    50821 tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa
    50881 catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt
    50941 gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga
    51001 tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc gtatgtggga
    51061 tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag cattgaaaga
    51121 tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga
    51181 gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt
    51241 caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg
    51301 tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag aagcagttat
    51361 cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga tggcgatgat
    51421 gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct
    51481 aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca cggcagaatt
    51541 gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg atatcccgtt
    51601 tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta
    51661 tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg gtacaagagc
    51721 gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc cagaattaga
    51781 ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg ctgcctgtga
    51841 aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta caaagaatga
    51901 tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc
    51961 acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag gctggttgca
    52021 agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg attatgggac
    52081 agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag caaaacggat
    52141 catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg agacaatcag
    52201 ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat tatcaggtaa
    52261 tacacatata atggaggaag tataatggga ttcagaaaag attttttatg gggcggtgct
    52321 acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact agcaaacgta
    52381 gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa aaaaatgttt
    52441 gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta tcatcgttat
    52501 aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc
    52561 tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg cttgaaattt
    52621 tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt gacgattacg
    52681 cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc cagagaactc
    52741 gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa
    52801 tattggctga cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg
    52861 ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag
    52921 ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta
    52981 ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg
    53041 gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa
    53101 tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga aatggaacca
    53161 ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc ttattatgct
    53221 tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa tatctttgct
    53281 tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga tccattaggc
    53341 ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt tattgtagaa
    53401 aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga tgattaccga
    53461 atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact agacggagtc
    53521 gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg taccggtgag
    53581 atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa tggcacatta
    53641 gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc aaatggagaa
    53701 aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag cggatttttc
    53761 gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc
    53821 tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt
    53881 attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc
    53941 gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc agaaatcgaa
    54001 aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat gtatgaaagt
    54061 gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa ggatcacggc
    54121 agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt cattatcgca
    54181 acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgaggc
    54241 gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct agtagtcatc
    54301 gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta
    54361 gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt
    54421 gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa
    54481 atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca
    54541 gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt tagagaagca
    54601 ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac gaatatgcca
    54661 ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt
    54721 ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt agaagaaaca
    54781 acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt
    54841 cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa
    54901 tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct
    54961 atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg
    55021 tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat
    55081 ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact
    55141 gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga
    55201 caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga
    55261 ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc
    55321 aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca
    55381 gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct
    55441 cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct
    55501 cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc
    55561 gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa
    55621 gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa
    55681 acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg
    55741 ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct
    55801 gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt
    55861 gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct
    55921 gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact
    55981 gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc
    56041 actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca
    56101 attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct
    56161 gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa
    56221 agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt
    56281 ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg
    56341 caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc
    56401 ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg
    56461 acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca
    56521 gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca
    56581 ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt
    56641 tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa
    56701 gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa
    56761 actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat
    56821 caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa
    56881 ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa
    56941 tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt
    57001 ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt
    57061 tgaaccagac actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact
    57121 ggagtgtgac aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa
    57181 aaatgattaa tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc
    57241 taaagcattc aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac
    57301 tacacatcct agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc
    57361 gatatcggaa taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat
    57421 attatttaaa atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca
    57481 cagctgtttg ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt
    57541 atttgctaaa aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac
    57601 atctgcttct aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat
    57661 tgctttgatc cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat
    57721 tggcttgata ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact
    57781 aaataattga tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa
    57841 tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt
    57901 ttcatctgtc acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat
    57961 agtagcttgc gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt
    58021 aacgatttct gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt
    58081 attataccat actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat
    58141 gaaaaaacag acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata
    58201 tactaaaaaa agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc
    58261 taaagaagtt tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta
    58321 ccggaatctc catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga
    58381 aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc catcaccatc attttatctg
    58441 tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca
    58501 gctaggtgga tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg
    58561 tcaataagga tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg
    58621 acggtcaaaa cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt
    58681 tcaataacaa ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca
    58741 gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca
    58801 aaagcgggta ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga
    58861 cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg
    58921 gagttggtag tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag
    58981 cgaaagataa ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc
    59041 agattaaaga aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa
    59101 tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg
    59161 aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg
    59221 aagaaattcg acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag
    59281 aatttggcaa agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc
    59341 aagtgaatgc gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac
    59401 agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa
    59461 gaataagatt ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt
    59521 gtttgatttg gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga
    59581 taggcgtaat aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca
    59641 agtcctaatg cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca
    59701 gtttgctgac tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca
    59761 gaaaagaaca tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag
    59821 gaaagcaaga ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat
    59881 tggatgatat tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc
    59941 agccatgtca tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca
    60001 acgagtccca tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca
    60061 ccgaagttac ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat
    60121 aaaggcatct tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact
    60181 tctaacgcaa cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta
    60241 aaaccaagat cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca
    60301 tcaaccgtgt tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct
    60361 gcagcagatt ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct
    60421 aataatgcaa gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga
    60481 tgtacagtaa ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat
    60541 gaatcatatt tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc
    60601 cgcggtgtac caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca
    60661 taccattttc cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca
    60721 gctaagatag ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg
    60781 atccagtttg atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg
    60841 aagaacatcc cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt
    60901 gtttcttcca taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc
    60961 agaaactaga aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa
    61021 aaaagccctt ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc
    61081 gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata
    61141 atggaggcag ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat
    61201 tcgatttatt gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta
    61261 ctgttttcct cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt
    61321 tttgaaatgc aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc
    61381 cagagaatac ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat
    61441 gatcagcttt ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt
    61501 ttcttggtac ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt
    61561 cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga
    61621 cggaagagtc tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt
    61681 taggtgcaca cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata
    61741 cacgtggaga aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag
    61801 tcattcggac attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg
    61861 tgacggcact gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg
    61921 aagagatcgt caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga
    61981 aatatgtcga atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag
    62041 gaaaaacttt tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa
    62101 aaatcatttt gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg
    62161 atctaaaaga aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt
    62221 ttggattaga tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag
    62281 cttacatgcg ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca
    62341 cgacagttgc tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg
    62401 tcaatggaga tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg
    62461 cagaacgaac attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg
    62521 ttgttcggca ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc
    62581 aagaataagc tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca
    62641 agaaaaactt ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg
    62701 cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc
    62761 agaagaaagt atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag
    62821 aaaactggca gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga
    62881 ccagaacaat cggatcaagc aactatttga attgatcgat aaaacgaatg atgccatcaa
    62941 caaatcttat gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac
    63001 tacagaagag cggatcgccg cgttgaagaa aaattttgaa aatgtgaatg cagaaaatgt
    63061 tgcgttttat caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac
    63121 tgagatccat actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca
    63181 agttcgtgaa agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt
    63241 gaatgtcaca ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt
    63301 gaatgacgta ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt
    63361 acaaccagtg atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc
    63421 ttcagctatg aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc
    63481 gctaattgcc atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact
    63541 tcaattccaa aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc
    63601 agtcagcata gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat
    63661 cccgctgttt tatcccgctg cttttgcacc acttgtttta agctttttct tgaaccgaag
    63721 agcagggatc atggctggta ttttccaagc agtatctgca cttttcattt tttatgattc
    63781 gattggcacg aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc
    63841 aacgattgtc agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat
    63901 tatttttcct gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga
    63961 tgcaacgact tggttaatgc ttgtctgcgg gttcactggt gcactcttct cattcttgct
    64021 gacgatcggt ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt
    64081 aaatgaactc agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg
    64141 tacttatcat catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg
    64201 cggacgttct ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca
    64261 tgctagcttt ttcgttgaga acttgccttc aggagcggaa aacccgcaca atttcttatt
    64321 gccagaagac agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga
    64381 agattataat atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct
    64441 aatgcgttat ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt
    64501 ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag
    64561 ttgtgaggct gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt
    64621 ccataatctt atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt
    64681 gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt ttaagtagca ccttccattc
    64741 tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag ataaaggaag aacaagaaga
    64801 atagaaagaa gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag
    64861 aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc
    64921 cagaggaaac agaaatgtct gttactttca tggacaatgc ggcaatccaa gtaatcaatc
    64981 gtgattaccg tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag
    65041 gcgaagacga aatcccagtg atttttgaag atggagagaa tccattgcct cgtgtactag
    65101 gagacatcat gatttctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat
    65161 atgatcggga acttggcttt ttagcggtac atggattctt gcatatcaac gggtatgacc
    65221 atatgacacc agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct
    65281 atggactcaa aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt
    65341 gctgtccaag gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg
    65401 ttcggtatcc ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta
    65461 tggctgcttt tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa
    65521 aatgtagtgg acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat
    65581 atggcagctg gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta
    65641 tttgtcccta aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac
    65701 ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga
    65761 accgaatcgt gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata
    65821 aaatccaagg agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa
    65881 tccataaacc aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga
    65941 aagaagtgga tgcagtctta tttatgatca gtgctgatca aaaacgcggc cggggagacg
    66001 attttattat tgaacgtcta aaaaacgtgc attctccagt ttatctgatt atcaataaaa
    66061 tcgataagat ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc
    66121 cttttgcaca agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg
    66181 atgtattagt cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg
    66241 atcatcctga atactttatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac
    66301 gagacgaagt gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg
    66361 ataaaatcca tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta
    66421 tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa
    66481 atcttttagg agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag
    66541 acaaaaaagt ctatctgcaa gactttggct accgaaaaga cgaatactga tattctgaac
    66601 ttcttatagg ttaagtatag aaataaactg gataaaaatc cgcacgttga gaaaaacggc
    66661 ggaagatcga aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt
    66721 aggagggaga atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat
    66781 tacaaagaaa aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt
    66841 tatatcaaag gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc
    66901 gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa
    66961 gacgtccatg cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacctat
    67021 ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa
    67081 tttacgcaac aagctctgca gcggatggat caaggagatg atgcagaaat catcacgaat
    67141 atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc
    67201 gtatgcgggg aaacaaaagg aaaatttgac tattcttcca aatctggcgg tgtgatatgc
    67261 gagaaacact ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc
    67321 gttcgaatgt tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa
    67381 acgaagcagg caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat
    67441 ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca
    67501 gagaaaaata tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca
    67561 aaaaaatcaa aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa
    67621 atagatcgtt cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta
    67681 cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga
    67741 cccaaacgat ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat
    67801 tcggaagtag ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga
    67861 ctttttgtcg ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg
    67921 caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt
    67981 ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac agaaaaagga gcaggtacga
    68041 tgagcccata tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag
    68101 agccatcccg ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc
    68161 atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag
    68221 aaagcttgcg tcttttagga attgatccat tagaacacga tattcgtttt gttgaagaca
    68281 actgggagaa cccatcgatg gggtgtgccg gtgtgggttg ggaagtatgg cttgatggga
    68341 tggagatcac acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta
    68401 cttctgagat cacttatgga ctagaacgtc ttgcttcata tattcaagaa gttgaaagtg
    68461 tctatgatct ggaatgggct gatggtgtga aatatggcga gatcttcaaa caaccagaat
    68521 atgaacattc caaatattct tttgaagtaa gtgaccaaga cttgctacta agcaactttg
    68581 accgttttga aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg
    68641 attatatttt gaaatgcagc catacattca atttattaga tgctcgcggt gcagtatccg
    68701 taacagaacg agcaggttat ctttcccgta tccgcaacat ggctcgtgct gtagccaaaa
    68761 tctttgtggc agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc
    68821 cagaaaacat gaaggaggct gaataagatg gcaaaagact tattacttga aatcgggctg
    68881 gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac aattagaaga aaaaacagca
    68941 aaatttttag atgaaaacca attgacttat gatacgatcg aaacgttttc tacaccgcgc
    69001 cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag cagacagtga agaagaagtc
    69061 aaaggaccag ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagctgaa
    69121 ggatttgtcc gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc
    69181 gtggaatatg tctatgtaac gaaatacact cacggcaaag aagcgaaaga agtattgaca
    69241 ggtttagcag atgtcattaa aagcctcact ttcccagtaa cgatgcattg ggcagaatac
    69301 gattttgaat atatccgccc tattcactgg atcgtggcat tgttagatga cgaagtaatc
    69361 ccgtttgaga tcttagatgt caaaacaggc agagcctctc gaggacatcg tttcttaggc
    69421 gaagatgtga catttgcgca tgcaaaagaa tatgaagaaa aattgaatga acaattcgtt
    69481 attgcttcac cgcaaaaaag acaagaaatg atcgttacac aagcaaatga attagccgaa
    69541 aaaaatcact gggttattga tctagacgaa gatttactag aagaagtcaa caatttagtc
    69601 gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat atctttctgt tcctgaagaa
    69661 gtactagtca cttctatgaa agaacatcaa cgttatttcg aggtacggaa cgatcaaggt
    69721 atgctgcttc ctcattttat ttctgctcgt aatggtgatc gggtaaaatt ggaaaatgtg
    69781 gttcgaggca atcaaaaagt attgacggct cgtttagaag atgcggaatt cttttataat
    69841 gaagacaaga aagcttcaat cgaccaatac gttgaaaaac taaaaactgt tactttccat
    69901 gaaaaaatcg gtagtatcta tgagaaaatg cagcgggtac aagtcattgc ccgtatcatt
    69961 ggcgaacaag taggactatc tgaggaagaa ttgatggatc tgcaacgagc tgctgaaatc
    70021 tataaatttg atctagtaac caacatggta ggggaatttc ctgaactaca agggatcatg
    70081 ggcgaaaaat atgctctgct gaaaggcgag aaacctgctg tagcaacagc gattcgtgag
    70141 cattacatgc ctatatccag tgaaggagag ctacctcagt ctacagtagg ttctgtttta
    70201 gcgattgcag ataaattaga tagcattttt tctttctttg cagttgggat gattccaagc
    70261 gggtcaaacg atccttatgc cttacgtcgc caagcatacg gtgtcgttcg gatcatcgaa
    70321 tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga tgttatcagt
    70381 aaagatacgg atcgtttcgg cattggttta tcagaagggc aacaacaggt gatcgacttt
    70441 atcaaaggcc gtctgcgcca attattgaca acgaagaata ttcgtcatga tgtcatcgaa
    70501 gctgttttga atgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc
    70561 aagcagcatc ttgcagatga agacttcaaa ccatcgatgg aagctttgac acgtgtcgtg
    70621 aacttagcga aaaaagcgga gcttgaaaaa caatcagaag tagaccctga actgttcgaa
    70681 aatgaagcgg agaaagaact gcacaaagca gtagaggcaa tgaaaaaaag ctttgctgac
    70741 caaacgatca acgaaaatta tgatcagctt gtaagccttc gcccactgat cgaaaattat
    70801 tttgatcaga cgatggtcat ggcagaagaa ccagcagtac gtaagaaccg attaacgcaa
    70861 ttaagtcaaa ttgctgccat ggctttgtcg ctagcaagtt tagatcaaat catcacaaaa
    70921 taatttctaa tatgtaaata aaccagagaa taggcacaaa a
//
LOCUS       DACDBG010000005        70234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDBG010000005 DACDBG010000000
VERSION     DACDBG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.005"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IVZ90_000307"
     CDS             complement(12..1535)
                     /locus_tag="IVZ90_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8261384.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IVZ90_000308"
     CDS             1787..2968
                     /locus_tag="IVZ90_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8261385.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IVZ90_000309"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IVZ90_000309"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8261386.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IVZ90_000310"
     CDS             complement(5053..7764)
                     /locus_tag="IVZ90_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8261387.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IVZ90_000311"
     CDS             complement(7774..8475)
                     /locus_tag="IVZ90_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261388.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IVZ90_000312"
     CDS             8661..9260
                     /locus_tag="IVZ90_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8261389.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IVZ90_000313"
     CDS             9341..10354
                     /locus_tag="IVZ90_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8261390.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IVZ90_000314"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IVZ90_000314"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8261391.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IVZ90_000315"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IVZ90_000315"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8261392.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IVZ90_000316"
     CDS             11802..13175
                     /locus_tag="IVZ90_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261393.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="IVZ90_000317"
     CDS             13532..15676
                     /locus_tag="IVZ90_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8261394.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="IVZ90_000318"
     CDS             complement(15728..16768)
                     /locus_tag="IVZ90_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8261395.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="IVZ90_000319"
     CDS             complement(17045..18847)
                     /locus_tag="IVZ90_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261396.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="IVZ90_000320"
     CDS             complement(18861..19613)
                     /locus_tag="IVZ90_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261397.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="IVZ90_000321"
     CDS             complement(19622..19984)
                     /locus_tag="IVZ90_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8261398.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="IVZ90_000322"
     CDS             complement(20160..21032)
                     /locus_tag="IVZ90_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8261399.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="IVZ90_000323"
     CDS             complement(21058..21570)
                     /locus_tag="IVZ90_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8261400.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22538)
                     /locus_tag="IVZ90_000324"
     CDS             complement(21708..22538)
                     /locus_tag="IVZ90_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8261401.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22554..23162)
                     /locus_tag="IVZ90_000325"
     CDS             complement(22554..23162)
                     /locus_tag="IVZ90_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8261402.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="IVZ90_000326"
     CDS             complement(23155..23472)
                     /locus_tag="IVZ90_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8261403.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="IVZ90_000327"
     CDS             complement(23628..24356)
                     /locus_tag="IVZ90_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8261404.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="IVZ90_000328"
     CDS             24515..25396
                     /locus_tag="IVZ90_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8261405.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="IVZ90_000329"
     CDS             complement(25633..26676)
                     /locus_tag="IVZ90_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8261406.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="IVZ90_000330"
     CDS             complement(26679..27347)
                     /locus_tag="IVZ90_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8261407.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="IVZ90_000331"
     CDS             27489..29825
                     /locus_tag="IVZ90_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261408.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="IVZ90_000332"
     CDS             complement(29940..30251)
                     /locus_tag="IVZ90_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261409.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="IVZ90_000333"
     CDS             30432..31907
                     /locus_tag="IVZ90_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8261410.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="IVZ90_000334"
     CDS             31954..32538
                     /locus_tag="IVZ90_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8261411.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="IVZ90_000335"
     CDS             32540..32959
                     /locus_tag="IVZ90_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8261412.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="IVZ90_000336"
     CDS             complement(33240..33524)
                     /locus_tag="IVZ90_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8261413.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="IVZ90_000337"
     CDS             complement(33876..34586)
                     /locus_tag="IVZ90_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8261414.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="IVZ90_000338"
     CDS             complement(34583..36055)
                     /locus_tag="IVZ90_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8261415.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="IVZ90_000339"
     CDS             36193..36666
                     /locus_tag="IVZ90_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8261416.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="IVZ90_000340"
     CDS             complement(36819..37370)
                     /locus_tag="IVZ90_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8261417.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="IVZ90_000341"
     CDS             37475..38005
                     /locus_tag="IVZ90_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8261418.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="IVZ90_000342"
     CDS             complement(38488..38931)
                     /locus_tag="IVZ90_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261419.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="IVZ90_000343"
     CDS             complement(38947..40107)
                     /locus_tag="IVZ90_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8261420.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="IVZ90_000344"
     CDS             40614..42314
                     /locus_tag="IVZ90_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261421.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="IVZ90_000345"
     CDS             complement(42416..43249)
                     /locus_tag="IVZ90_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8261422.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="IVZ90_000346"
     CDS             complement(43553..45718)
                     /locus_tag="IVZ90_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261423.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="IVZ90_000347"
     CDS             45978..48272
                     /locus_tag="IVZ90_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8261424.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="IVZ90_000348"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="IVZ90_000348"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8261425.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="IVZ90_000349"
     CDS             48923..49930
                     /locus_tag="IVZ90_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8261426.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="IVZ90_000350"
     CDS             49952..50971
                     /locus_tag="IVZ90_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8261427.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IVZ90_000351"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IVZ90_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8261428.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="IVZ90_000352"
     CDS             complement(52729..54150)
                     /locus_tag="IVZ90_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261429.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IVZ90_000353"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IVZ90_000353"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8261430.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="IVZ90_000354"
     CDS             complement(56561..57817)
                     /locus_tag="IVZ90_000354"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8261431.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58397..59698
                     /locus_tag="IVZ90_000355"
     CDS             58397..59698
                     /locus_tag="IVZ90_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8261432.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="IVZ90_000356"
     CDS             complement(59767..60366)
                     /locus_tag="IVZ90_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8261433.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="IVZ90_000357"
     CDS             60620..61501
                     /locus_tag="IVZ90_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261434.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="IVZ90_000358"
     CDS             61514..63124
                     /locus_tag="IVZ90_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8261435.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="IVZ90_000359"
     CDS             complement(63201..63860)
                     /locus_tag="IVZ90_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8261436.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="IVZ90_000360"
     CDS             complement(64143..64808)
                     /locus_tag="IVZ90_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261437.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IVZ90_000361"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IVZ90_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8261438.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IVZ90_000362"
     CDS             complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IVZ90_000362"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8261439.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IVZ90_000363"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IVZ90_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8261440.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="IVZ90_000364"
     CDS             67122..67442
                     /locus_tag="IVZ90_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8261441.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="IVZ90_000365"
     CDS             complement(67554..68423)
                     /locus_tag="IVZ90_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8261442.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69793)
                     /locus_tag="IVZ90_000366"
     CDS             complement(68519..69793)
                     /locus_tag="IVZ90_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8261443.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(69819..>70234)
                     /locus_tag="IVZ90_000367"
     CDS             complement(69819..>70234)
                     /locus_tag="IVZ90_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261444.1"
                     /translation="VALLGWMIVVLPFIWVFFPLTRPERAKDFSLYAFLSEKKMLYFL
                     IGFFVLIFFSFLITSFVLTRWNNRNLYKKVNKSFEYEDEKLKKRQELLEEVYTERFGT
                     KEERETVRFYSVSEEKNLDTTFIADLYKENGVELK"
BASE COUNT        20898 a        14399 c        12657 g        22280 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc acta
//
LOCUS       DACDBG010000006        64118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDBG010000006 DACDBG010000000
VERSION     DACDBG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.006"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(87..614)
                     /locus_tag="IVZ90_000368"
     CDS             complement(87..614)
                     /locus_tag="IVZ90_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8261445.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(681..2183)
                     /locus_tag="IVZ90_000369"
     CDS             complement(681..2183)
                     /locus_tag="IVZ90_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8261446.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2441..3562)
                     /locus_tag="IVZ90_000370"
     CDS             complement(2441..3562)
                     /locus_tag="IVZ90_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8261447.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3688..4629
                     /locus_tag="IVZ90_000371"
     CDS             3688..4629
                     /locus_tag="IVZ90_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8261448.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4779..5675)
                     /locus_tag="IVZ90_000372"
     CDS             complement(4779..5675)
                     /locus_tag="IVZ90_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8261449.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTSFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5944..7284
                     /locus_tag="IVZ90_000373"
     CDS             5944..7284
                     /locus_tag="IVZ90_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261450.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7502..7945
                     /locus_tag="IVZ90_000374"
     CDS             7502..7945
                     /locus_tag="IVZ90_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8261451.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8210..9628)
                     /gene="nhaC"
                     /locus_tag="IVZ90_000375"
     CDS             complement(8210..9628)
                     /gene="nhaC"
                     /locus_tag="IVZ90_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8261452.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10031..11254)
                     /locus_tag="IVZ90_000376"
     CDS             complement(10031..11254)
                     /locus_tag="IVZ90_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8261453.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11285..12124)
                     /locus_tag="IVZ90_000377"
     CDS             complement(11285..12124)
                     /locus_tag="IVZ90_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8261454.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12141..12821)
                     /locus_tag="IVZ90_000378"
     CDS             complement(12141..12821)
                     /locus_tag="IVZ90_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261455.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(12823..13896)
                     /locus_tag="IVZ90_000379"
     CDS             complement(12823..13896)
                     /locus_tag="IVZ90_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261456.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     gene            complement(14542..15213)
                     /locus_tag="IVZ90_000380"
     CDS             complement(14542..15213)
                     /locus_tag="IVZ90_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8261457.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(15300..17978)
                     /gene="mgtA"
                     /locus_tag="IVZ90_000381"
     CDS             complement(15300..17978)
                     /gene="mgtA"
                     /locus_tag="IVZ90_000381"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8261458.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            18637..19074
                     /gene="ndk"
                     /locus_tag="IVZ90_000382"
     CDS             18637..19074
                     /gene="ndk"
                     /locus_tag="IVZ90_000382"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8261459.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19096..19962)
                     /locus_tag="IVZ90_000383"
     CDS             complement(19096..19962)
                     /locus_tag="IVZ90_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8261460.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20122..22215)
                     /locus_tag="IVZ90_000384"
     CDS             complement(20122..22215)
                     /locus_tag="IVZ90_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8261461.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
     gene            complement(22691..23314)
                     /locus_tag="IVZ90_000385"
     CDS             complement(22691..23314)
                     /locus_tag="IVZ90_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261462.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23473..24870)
                     /locus_tag="IVZ90_000386"
     CDS             complement(23473..24870)
                     /locus_tag="IVZ90_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8261463.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(24902..25873)
                     /locus_tag="IVZ90_000387"
     CDS             complement(24902..25873)
                     /locus_tag="IVZ90_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8261464.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26247..26672
                     /locus_tag="IVZ90_000388"
     CDS             26247..26672
                     /locus_tag="IVZ90_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261465.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(27160..27861)
                     /locus_tag="IVZ90_000389"
     CDS             complement(27160..27861)
                     /locus_tag="IVZ90_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8261466.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27877..28317)
                     /locus_tag="IVZ90_000390"
     CDS             complement(27877..28317)
                     /locus_tag="IVZ90_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8261467.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28317..28892)
                     /locus_tag="IVZ90_000391"
     CDS             complement(28317..28892)
                     /locus_tag="IVZ90_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8261468.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(29739..30218)
                     /locus_tag="IVZ90_000392"
                     /pseudo
     CDS             complement(29739..30218)
                     /locus_tag="IVZ90_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30377..31078)
                     /locus_tag="IVZ90_000393"
     CDS             complement(30377..31078)
                     /locus_tag="IVZ90_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8261469.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(31186..32595)
                     /locus_tag="IVZ90_000394"
     CDS             complement(31186..32595)
                     /locus_tag="IVZ90_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8261470.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(32643..34508)
                     /locus_tag="IVZ90_000395"
     CDS             complement(32643..34508)
                     /locus_tag="IVZ90_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261471.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(34629..35480)
                     /locus_tag="IVZ90_000396"
     CDS             complement(34629..35480)
                     /locus_tag="IVZ90_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261472.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(35790..36503)
                     /locus_tag="IVZ90_000397"
     CDS             complement(35790..36503)
                     /locus_tag="IVZ90_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8261473.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(36571..36897)
                     /locus_tag="IVZ90_000398"
     CDS             complement(36571..36897)
                     /locus_tag="IVZ90_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8261474.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(37020..37247)
                     /locus_tag="IVZ90_000399"
     CDS             complement(37020..37247)
                     /locus_tag="IVZ90_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8261475.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(37569..38822)
                     /locus_tag="IVZ90_000400"
     CDS             complement(37569..38822)
                     /locus_tag="IVZ90_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8261476.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            38883..39623
                     /locus_tag="IVZ90_000401"
     CDS             38883..39623
                     /locus_tag="IVZ90_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8261477.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(39709..40467)
                     /locus_tag="IVZ90_000402"
     CDS             complement(39709..40467)
                     /locus_tag="IVZ90_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8261478.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(40464..41492)
                     /locus_tag="IVZ90_000403"
                     /pseudo
     CDS             complement(40464..41492)
                     /locus_tag="IVZ90_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(41512..44679)
                     /locus_tag="IVZ90_000404"
     CDS             complement(41512..44679)
                     /locus_tag="IVZ90_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261479.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(44903..45787)
                     /locus_tag="IVZ90_000405"
     CDS             complement(44903..45787)
                     /locus_tag="IVZ90_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8261480.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(45882..46319)
                     /locus_tag="IVZ90_000406"
     CDS             complement(45882..46319)
                     /locus_tag="IVZ90_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261481.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(46536..47447)
                     /locus_tag="IVZ90_000407"
     CDS             complement(46536..47447)
                     /locus_tag="IVZ90_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8261482.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(47904..48332)
                     /locus_tag="IVZ90_000408"
     CDS             complement(47904..48332)
                     /locus_tag="IVZ90_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261483.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(48360..48998)
                     /locus_tag="IVZ90_000409"
     CDS             complement(48360..48998)
                     /locus_tag="IVZ90_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261484.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(49002..51038)
                     /locus_tag="IVZ90_000410"
     CDS             complement(49002..51038)
                     /locus_tag="IVZ90_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8261485.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            51164..51829
                     /locus_tag="IVZ90_000411"
     CDS             51164..51829
                     /locus_tag="IVZ90_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8261486.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(53269..57666)
                     /locus_tag="IVZ90_000412"
     CDS             complement(53269..57666)
                     /locus_tag="IVZ90_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8261487.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
     gene            complement(57644..58411)
                     /locus_tag="IVZ90_000413"
     CDS             complement(57644..58411)
                     /locus_tag="IVZ90_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HAP8261488.1"
                     /translation="MVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGIKEACEE
                     LQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGVELLEDN
                     AQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKRTPDDNP
                     IIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLDLFSFDD
                     EVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            complement(60185..60961)
                     /locus_tag="IVZ90_000414"
     CDS             complement(60185..60961)
                     /locus_tag="IVZ90_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261489.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            complement(61162..61488)
                     /locus_tag="IVZ90_000415"
     CDS             complement(61162..61488)
                     /locus_tag="IVZ90_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261490.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(61534..61896)
                     /locus_tag="IVZ90_000416"
     CDS             complement(61534..61896)
                     /locus_tag="IVZ90_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8261491.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(61933..62148)
                     /locus_tag="IVZ90_000417"
     CDS             complement(61933..62148)
                     /locus_tag="IVZ90_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8261492.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            62274..62609
                     /locus_tag="IVZ90_000418"
     CDS             62274..62609
                     /locus_tag="IVZ90_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8261493.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(62685..>64118)
                     /locus_tag="IVZ90_000419"
     CDS             complement(62685..>64118)
                     /locus_tag="IVZ90_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261494.1"
                     /translation="EDNMLDITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKN
                     DDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNLRCLFHDDHNPSASI
                     FTSKTGHSIFYCHSSNCGISENFIGVVMLILEKDSRVETFNWLLENLDLYPTHFRKLK
                     EESKVLFDTLESMEDKLFRTINNYLEEMRRVYDALLSDVNFFDDSKESVTCILSGEQL
                     AKKMSSYYGKTYDIDKWNKLLSFMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNF
                     NHKRSNVYRLDVFNATSVIQKLNEDILPLLKKVHFTYQYFSYEWVKICFNEKEAKRVF
                     PQNFRSELSKEKQLILDEAHEVLQDLSWQGTYLMTEKELINTVCERLRNVGNNSVENA
                     LKENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGIDEVIKELIASGVEL
                     VPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT        18666 a        13300 c        10528 g        21624 t
ORIGIN      
        1 caaaagaatt gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat
       61 tttatttaaa taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc
      121 atgaatgtaa agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc
      181 aaggcattat ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat
      241 aaaggaacga tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg
      301 aacgctaaac gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca
      361 aaatccattt ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact
      421 gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca
      481 ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc
      541 ggcacatctg cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc
      601 gttacataaa gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt
      661 tgttcttgag attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta
      721 tccaggaagg tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt
      781 tgttctaatt gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat
      841 agttcagctg gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc
      901 atctgctcga tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc
      961 aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg
     1021 tcttcataat tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct
     1081 ataaccttgc cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg
     1141 ttaggacctg aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat
     1201 aacagatttt cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac
     1261 gcttggctat tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc
     1321 tcgctgatct gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca
     1381 gcatcagcac ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact
     1441 tgtttctttg ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt
     1501 ttctttagcc tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca
     1561 cgaagttttt tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt
     1621 ttttcgattg ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc
     1681 acgatgaagc cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata
     1741 tccaaatgat tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt
     1801 gccagtagga cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt
     1861 tcaggatctg tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta
     1921 atatcattta acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag
     1981 gcaaattctt gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc
     2041 agtaaagttg tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca
     2101 aaatttagat ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga
     2161 ttttttattt cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt
     2221 cagaactggc taaagaggat acaagggttg gatacacaca aaaagaccca tacatcgaaa
     2281 aaaccaagcc aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg
     2341 aatgcagctt tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat
     2401 atttgaaatt acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga
     2461 aaaatctgct aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca
     2521 ttgcttcttc taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag
     2581 aaagtccttc tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt
     2641 tttttgcaag cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc
     2701 gcccttctcc tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt
     2761 ggctgactaa atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa
     2821 taccgcctgc tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta
     2881 tttgctgagc aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc
     2941 ctttttgtct tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat
     3001 ctgcagcgat aatcaatttt actttagaaa caagaacgtt tcttcgatca aaatgaagtt
     3061 ttttagtaga taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt
     3121 ggagtaatcc tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt
     3181 ctacttcttg aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc
     3241 ggatagtttg atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga
     3301 tgactgcggt gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga
     3361 ataagagatc catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt
     3421 ctgcagtatt ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt
     3481 cccagccatt tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat
     3541 caattgccgc caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt
     3601 ttcatttttt tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga
     3661 actcattgaa taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg
     3721 ggcagccgaa tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt
     3781 tggcctgctc acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa
     3841 gaagtggtcg caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac
     3901 gtggacttga tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc
     3961 caatccatta ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa
     4021 gatgtactgg aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca
     4081 gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag
     4141 aacttggaac cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca
     4201 aatttaaatc cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta
     4261 aatggaacat tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg
     4321 caaaaagcgg cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca
     4381 aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat
     4441 cctgacaaca tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt
     4501 ttgactgaaa tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt
     4561 gctacaccat attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc
     4621 gcagaataaa aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta
     4681 agcccaataa aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg
     4741 tttgtttcag tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag
     4801 aggtcatatt cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa
     4861 ttttctgcat cataaaattc caatagctct gataggattg gcttaaatga tgtatgcaga
     4921 aattccggta attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa
     4981 aaatgataat gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat
     5041 tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc
     5101 gattcccctg tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct
     5161 cgatgtggaa gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag
     5221 ctttctgctc gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt
     5281 tcgctagaag aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt
     5341 tcttttgctt ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc
     5401 tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt
     5461 gcattggtaa atgggcgcac gcgtcgagcc atctccatat taaatccata atctaaaaaa
     5521 acgatcagct gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt
     5581 aaatcttttc cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta
     5641 ttttttgcaa ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca
     5701 ctttttccta tgatagcctg tttaaatagt aaaaagccag taaaacgctt cctatatttg
     5761 tatgaaaatt taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt
     5821 cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt
     5881 taaatattgc tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca
     5941 tttatggaag ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct
     6001 ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc
     6061 ggcccttctg tattgattgc ttttttgatt ggcgggctga ttttatatat cgtgatgaaa
     6121 ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata
     6181 cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg
     6241 attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg
     6301 cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt
     6361 tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc
     6421 attatcttac taatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc
     6481 gttttcccta cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag
     6541 ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct
     6601 atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagccat caaaggtgtc
     6661 atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct
     6721 tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat
     6781 atcccatttg cagcggatat cgtcaatttt gttattgtcc ttgctttgtt ctcatctatc
     6841 aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt
     6901 tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt
     6961 tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt
     7021 ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg
     7081 gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt
     7141 gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta
     7201 actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat
     7261 gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata
     7321 gatggaacta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa
     7381 taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg
     7441 attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta
     7501 aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg
     7561 tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt
     7621 catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat
     7681 gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg
     7741 ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat
     7801 gattcctcat cattctatcg ctatcctgac aagtaaacga gctgatttaa aagatcctga
     7861 agtaaaaaaa ttagcggaag aaatcatcca ggcacaagaa gaagagatcg acaaaatgaa
     7921 aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc
     7981 agaagtgatc agcttcaaga aataagaaga agctacttct ggaacaccgt gaataagggg
     8041 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct
     8101 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcgt
     8161 aaaagggatt gtgacaaaaa aagtcttgtc acaatccctt tttatgtttt tattgttcta
     8221 attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac
     8281 ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca
     8341 agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag
     8401 gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat acagttggat
     8461 caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga
     8521 attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaatt
     8581 ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt
     8641 cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac
     8701 tttcgattcc tccgcgacta agcagtgaat cgatttgttg gtttccagta tttgaaacaa
     8761 accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa
     8821 tcattcctac tgctacagcg atattcaaca aaatcgtcaa aattgccggc acctttttcc
     8881 atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg
     8941 cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgcc
     9001 ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg aacagttgac cacatcatat
     9061 gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg
     9121 ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg
     9181 ctggatttag gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa
     9241 cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga
     9301 caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg
     9361 cttgtatcca tacagcaatc aatgaaccta ctaaaataaa aataaagatt gggatgattc
     9421 ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca
     9481 ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta
     9541 atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta
     9601 aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt
     9661 tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgctatt cctcctttat
     9721 aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta
     9781 aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat
     9841 tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac
     9901 cgctactgcg tgaaaagttg acttgtatga agtttacctt ccataagatc atttgtcaat
     9961 gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc acacacaagt
    10021 ttatttttta ctagaaagta gcaccctctt gtttttttct tgctactatt tcttgatgtt
    10081 cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga
    10141 gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga
    10201 attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg
    10261 gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt
    10321 ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag
    10381 aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc
    10441 ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg
    10501 tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg
    10561 cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc
    10621 gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt
    10681 caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg
    10741 ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga
    10801 catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt
    10861 cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaata aattgctcca
    10921 ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa
    10981 tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggtccgggt ttattggaaa
    11041 tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccctt
    11101 ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag
    11161 gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat
    11221 ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt
    11281 tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct
    11341 cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat
    11401 ttttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca
    11461 ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc
    11521 tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt
    11581 cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt
    11641 ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat
    11701 ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca
    11761 aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag
    11821 ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa
    11881 tcgatccatc tgctaaagcc gcatttggtt gattgtaatc tgtaaatttc acgtattcca
    11941 aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc
    12001 caataattcc tagttttacg gtagtttctt ttttgtcagt tgtatctgct tttccttggc
    12061 tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt
    12121 tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat
    12181 tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg
    12241 aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca
    12301 gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca
    12361 ggcaaacctt ctaacaaata cacacgtaaa ataataccga ttggactggt tcccatcgct
    12421 tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca
    12481 aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt
    12541 ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa
    12601 cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca
    12661 ttagggcgtg tcactactaa cgtcacacct aagatgattc cgatcatccc gccaatgaat
    12721 gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc
    12781 aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat
    12841 cactttgata cgactatcta attcgttttt ttgtaaaata tttattttta caccagattt
    12901 gatcaaataa tccaatgctt tttgccgctg tttggtttct ccagctaaag taacgatcaa
    12961 actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac
    13021 ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact
    13081 taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt
    13141 ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc
    13201 aggattgctg aagatctgga tgctactacc tttttcaatg attcttccat cttccataac
    13261 cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt
    13321 taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg
    13381 atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg
    13441 tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt
    13501 ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga
    13561 ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag
    13621 attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga
    13681 gagctgactc aaattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa
    13741 attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac
    13801 atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg
    13861 tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa
    13921 agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac
    13981 ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag
    14041 atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca
    14101 ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt
    14161 ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaatccttt tactaacata
    14221 aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc
    14281 ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg
    14341 aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac
    14401 tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa
    14461 atcgatattt tgttgttggg attaatctac atgaggaaaa ctaaaaagtc aagcacgaaa
    14521 ttcaaattat tttttctata tttatttttc tgttttttta tctaacattt caataacagt
    14581 tcgaagatct tttccttcag caagaagttt caatttttcc ttttcttcga cggtataatt
    14641 tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt
    14701 tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc
    14761 caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt
    14821 aaacgtgtca aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt
    14881 aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc
    14941 ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc
    15001 acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc
    15061 aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat
    15121 atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa
    15181 caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat
    15241 acacatagtt tagcacgact ggtccaaaaa taacagaagc aaagaaccag ccttctcgtt
    15301 tataagagtt cttggaatcg atggatatag attttcttca cagctgtcac aagcgccaga
    15361 taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct
    15421 aattcagcac caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg
    15481 ataacaaaca tggcaaatgt tacgttgctt tggataaatg gtactttcgg tgtacgcagt
    15541 gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat
    15601 gcgatgaaaa tcacttgctg tgaactatcc aatgtatgga aacttccgcc aactacagca
    15661 ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttggg
    15721 ccaaagtaaa ccatgaattt cccaatacta gatgcattcc atttttttgg ctcgtccaaa
    15781 tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata gattagattc
    15841 aaaaacagaa gttgtaaagg cagcattggt aaaaatggca ggaacatact tgcaatcagc
    15901 acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc
    15961 ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa
    16021 aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata
    16081 tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga
    16141 ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat
    16201 acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca
    16261 ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat
    16321 tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct
    16381 tgtttcgttg tttcttttgg tggatcaagg aaagtaagat aaccaatcaa aaccatgtct
    16441 gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca
    16501 atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt
    16561 tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt
    16621 tcttcaatcg cgccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca
    16681 ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta
    16741 tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc
    16801 cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct
    16861 tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc
    16921 gtgcataaca catcgattgc accaaagttt tggatggcat tcaaattttt gatcactgtt
    16981 ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc
    17041 atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc
    17101 ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc
    17161 agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat
    17221 agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc
    17281 gcacttccac ttatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt
    17341 tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat
    17401 gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata
    17461 tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg
    17521 tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc
    17581 gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat gaccatagtg
    17641 aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg
    17701 atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc
    17761 aatatttcta cgatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt
    17821 tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcgtg gaatagttca
    17881 aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt
    17941 ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct
    18001 ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat
    18061 agccttggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat
    18121 ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata gttattttcc
    18181 caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct
    18241 ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc
    18301 atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat
    18361 gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc
    18421 agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta
    18481 atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta
    18541 aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg
    18601 ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc
    18661 aagccagacg gcgtgagacg ccatttagtc ggaagcatca ttcaacgttt tgaagcaaag
    18721 ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat
    18781 tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt
    18841 cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt
    18901 ggggcaacaa aagctgtcga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg
    18961 gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc
    19021 cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa
    19081 ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga
    19141 cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt
    19201 cgtctttttt tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt
    19261 ttcatagatc actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac
    19321 actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa
    19381 agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat
    19441 tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat
    19501 agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca
    19561 ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag
    19621 actcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat
    19681 ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt
    19741 taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt
    19801 cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc
    19861 aatcccgact agagaagtct tagaacagat tccttccgct aattgggact gtgtcaactt
    19921 cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa
    19981 tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa
    20041 taaaaaaatg tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact
    20101 actgaaggtt ggtttgattg tttattgatt atctttttgc tgttcaacgt ctgttcttgt
    20161 tactggatat aagttggttg ttgttttatt gccatttttt gaaaagacac ttgtcgaatg
    20221 ttcgcctttt tgcttttcga tggctatgtt acgttctgct tcatttttat agtcatattt
    20281 tttcggatcg atcgtttcaa atccgtttgg cacataaaaa cgcaagagat tttcttgatt
    20341 cagcttatct gaaagactta aagcagattt gactttttcc tgatctttgg aaatttcctc
    20401 gtcgattcct gctgctttcg tatcgatgat ttctcctgtt tggttattgt aagcttttcc
    20461 tcctaagatg gtgtatttgg gtgtcacgaa gttcccattt cgaaaagcca ctacttgatc
    20521 atgctgagga gataataaat ccgtaccaaa atggagatag tctttatcat cgattcccaa
    20581 gagatgaagt aatgtcggca aaacatcgat ttctccaccg tattcgtgat tgactgaccc
    20641 tttagtataa ccaggtatat gaatcattaa tggcacacgc tgcatttgtg cgttgtcaaa
    20701 ttcattccat gtatctgggt cttttccaag agcacttgct aagtctttat tatctgtatt
    20761 tgagatgcca aaatgatccc cataaatcac aaagatcgaa ttttggtaaa tagcggattt
    20821 tttcaggtac gtaaaaaatt gttccaatga ctgatccaaa taatgtgccg ttcgaacata
    20881 gttatctact gtgctatttc cagtgttcaa agatggaaaa tcgaaattct tatcatccgt
    20941 ataataaggc gtgtgattgg ttacggaaag gaatttcgtg tagaacggct gttgtagatg
    21001 ttcaagatat tgggcagatt cactgaataa atctttatct aatattccat aacctagcgt
    21061 ttcatctgat tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata
    21121 tacatgatct ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc
    21181 ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca ttatctgacc caagttctgt
    21241 gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact
    21301 tgtctttccc tgtcctactt catgaaaaaa attatcaaag ctgatcgttg cttgattctg
    21361 gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa
    21421 ctgctggaaa ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt
    21481 ttcgggattt ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt
    21541 actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccat cataaatagt
    21601 atacgcatcg attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa
    21661 ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca
    21721 ggtcacagcg atagccatcg gtttgctgac aggttgactt ttgataggta ttttcttgat
    21781 cgtcaaccaa accagtatcc caatgaacgt agcaatatcc agccagtaga ggacatcatg
    21841 gggtttcatc aaagaaaagg agcttcctga taacccttgc gacactttcg aaaatcccaa
    21901 tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatgt tcaagtacaa
    21961 caaaagcgtg tttaaaatat caatgatcaa aagaatggct aatgctggct ttggcttttt
    22021 aatatacaaa gaaaggccaa aaaacaataa ggtagttgca atcgggttga tccacaagat
    22081 aaagtactgg atcgtacctt ccacacctaa cgaaaaatct gcatagtaag caataatcgt
    22141 cttcatccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt
    22201 tttaagttgt gtcacgtata tcacttctct ctattcacat ttttccttat tttgctcctg
    22261 aaaaatgaaa attccctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat
    22321 tattccgtgc acttttcaca gcataaaacc gctgtgatat aagcgattga aacagtaagg
    22381 tgataaaagt cttgtcgcag ctcctgttta gttgatgcag gacagctttt tcacaacttc
    22441 ttctttaaac ggtgttctcc tgccaactcc tcggccacaa gtcacaatat ttataaaaca
    22501 tgagaagctg ttttttgaaa ttgttccttg tggctcagag ttaaacggca agctcaagac
    22561 ctcttgatac acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat
    22621 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
    22681 cctctgtttt tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa
    22741 ttgaggcttg attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac
    22801 ttgttcctgt cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg
    22861 tctttttcca ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag
    22921 cggtgatcac ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca
    22981 aaacggtcaa atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt
    23041 catcgatatt aataaattga ttttcttctt ttccttttgg caaatagatg tccaattcgc
    23101 ctttttcgta attggcagtt attttatact ccgcccacca ataagttgag aattcttttt
    23161 tgaaattatc tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat
    23221 ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata
    23281 accaaaagag aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt
    23341 gactattcta ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta
    23401 aataattgaa cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc
    23461 aactattctt tcttaagcct caaatccgtt tttatctgat acgtctttga accagtgacc
    23521 cgattttttg attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg
    23581 attcttatat gcattggtcc atgaccagtt atccatacat gtccacatat gatagcctaa
    23641 acaattggca ccttcttgaa ttgcttgatg gacatatttc agatgagatt ggacaaattc
    23701 gatccgataa tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac
    23761 acccataccg ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat
    23821 caaaatatca tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg
    23881 accaggcatt tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat
    23941 tggtgtttct tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac
    24001 tgtattttct ttgatgaccg tcaaatcctc tggatggatc acaggcatga gatcaagttc
    24061 ttttactatc tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga
    24121 acgattgaag aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc
    24181 tcttggatag cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc
    24241 atggtaagat ttgatggcta acgctgaggc caacgcttca tgatagccaa cttggatcgc
    24301 ttttttcata tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg
    24361 aacgattggt tcattatgag taaaccattt ttttactttc gaaccgaaca attcaaaaca
    24421 acttttggca tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc
    24481 ttgcagatac atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc
    24541 aatcaactca tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga
    24601 ttcatcaggt atcaagcggc tccattggat agatgttcga aatgaattat gcccagtctg
    24661 tttcatcaac tgtacatcct cttgatactt cgtataaact tgtgaagttt tttcaggtcc
    24721 tacttggtca aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc
    24781 ttttccatca cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca
    24841 aaaatttgct ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt
    24901 tttagttgat tccatgtatg atattttcca tcacaattac tttttcattt tcttcctctt
    24961 cttttttgat tcgtttcaac agctgttctg cggcttgttc cccgatttta tctgttggtg
    25021 gtgtaatcgt gattttcgga aggatgctca aatcgttttt atccaaataa tcataatgac
    25081 taaacgcaat atccttcccg attctgatac cttcttgatt gaaacattcg atagcaccga
    25141 tcgacatatt ataattgctg acaaaaatcg ctgttactcc tttttctaat aattcttttg
    25201 ccccataata gccacttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg
    25261 gatcattttg taccgcttct tttgctcctg ctaaacgttc tcttgctacg tagtctgttt
    25321 gagtggcagc aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt
    25381 ttgctacact ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctgggc
    25441 aattcaatgc gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt
    25501 cttcggataa gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt
    25561 gttcaatttt ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt
    25621 cagctactct ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta
    25681 tcaaaagtcc aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt
    25741 tatagtccaa cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct
    25801 gaccattcaa gtaacgggaa actgttccca ctgaaacacc agccatctta gccacgtcac
    25861 ggatcgatgc catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt
    25921 cgattatgat gaccgctttc tttgcatagt gttgcttgtc atcaatatcc atataactaa
    25981 agtcttcagt agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg
    26041 gttcattttt aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttcct
    26101 aaaatggcga attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt
    26161 gttttgtgcg tttttctctt ccatccgttt gaagttttac tttccaatgg tataatttct
    26221 ttatcctatc aaatggggga agtattttga agaaattaat cgtactattt attttgtctg
    26281 cgggactttt gacattgact ggctgtaatt tgtttggcgg ctctgaccaa tcctcggatg
    26341 aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga tatcactaac agtgtatata
    26401 cgctaaaagt acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa
    26461 aaatggaatg gacacgcacg tatcagacac aagaaactga ttcttctgaa tcatctgaag
    26521 aaaaaacagt gctgacagac atcaagatta ctaccaaaga tgacgaatat attatttcag
    26581 gaaaagaaaa taaaaaagaa gcaaccatca catttaccaa aatcggtaat taccgtttga
    26641 aggatgagga tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc
    26701 tccccttctt ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg
    26761 atcgatagtc ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat
    26821 gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa
    26881 acatttgaat cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca
    26941 agcaaaactc actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa
    27001 gcttcatcag taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt
    27061 atttatttca aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca
    27121 cgtagtaatt tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt
    27181 tgcatgctgg ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctagttt
    27241 ttcttggatc tgatggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt
    27301 atccccatcc acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg
    27361 ggtgttcaac gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc
    27421 acgcccacgt tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat
    27481 caaatcaggc cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa
    27541 gctatcgtac tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa
    27601 caacaggcta tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt
    27661 tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc
    27721 aggatttgaa taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt
    27781 agaatcaatc tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc
    27841 tgaaatcaaa atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt
    27901 ttcaacaggc aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt
    27961 atggagactt tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat
    28021 cggttttttc aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg
    28081 atgctcaaag tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt
    28141 ttcgtatact tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg
    28201 acgtggagag tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact
    28261 tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat
    28321 tttgcctcct tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt
    28381 tccaatggat tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct
    28441 tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt
    28501 ccgattggct tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc
    28561 tccaatgtac ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat
    28621 acctttcttt catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat
    28681 tcagtttcaa aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact
    28741 gttttactaa tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct
    28801 tcttgtgcca tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca
    28861 aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct
    28921 atcttcattc tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg
    28981 tatataggat agacaccact atatatagta tttagaaaaa aacatttttc agctatttta
    29041 tagataaaac aaaaataaat aacgtgtttt catcgatgtg aaatgactta cttatagaag
    29101 atactgggat ttcgtcctat atgccaataa cgtgtattat tttaaacaac aaatacttgt
    29161 ttcttttatt aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga taaatgatgt
    29221 ttctctaaat aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt ttgttcatca
    29281 gcttctccta aaaatctttt tgttaacgat aaatagctgg atttgtaaaa atatcacttg
    29341 aatcgtcact gctggaagaa aattcagtga taaccgcacc tgttgcgcct gcctggaact
    29401 agtgtttgat attgggctcg aacgtgtact gatctctagc atgaagaata atttcttttt
    29461 ttactgtata ataactttct ttcctttttg gaatagtaca ggaaagtttt tttgtttcct
    29521 ttccttcgac ataaggtata ccagaccctt tcgacaacga aaggttctca tttttccttc
    29581 atcgtcattt caaggagggt gccgatgctc tggacagttt cgatcgctcc taaaccaaaa
    29641 tctgtatatt cgattctttc taactctgat tctttcatac cattcctgcc tcaaggtaca
    29701 tctattgtac ttttttcttg taatccactc tgcctctcct aactaattgt caaatcatcc
    29761 caagatatgt cttcttcctc tttttcttct tggatgattt ctgcattcgg atctactggt
    29821 tttttcaata ataaaatggt tattcctgtg acaaaagaac cagctaacaa tgcaccaatg
    29881 aatgcccatg gtctggtcat cgtagcgact gcaaacattc ccgatgccgg aatacggcta
    29941 tctgcgcccc aaaacatgct taatccgcca cctactgtcc caccgactcc cgttgctatg
    30001 acattgcgtg ccaagtcgtt caatacgata gggaaacatc cctctgtgat ctgacagatc
    30061 cccataggaa aagcggtctt caatgtatcg atttcttgac gattgagtat atttttacga
    30121 atgattttcc ctaagaaata agcgatcgtc aagccgaatg gggttaccat cgaagctaaa
    30181 atcaatacag tgattggttc atagattcct tctgaaaatc gtgccacacc cccttatcca
    30241 gaataaatgg taatccagaa acagccgtgt atctgcggta ataagcctaa ccaaacgaaa
    30301 aatatgagaa gctatttttg tttggctgtt cttattactt gatgcagggc agctttttca
    30361 caacctcttt ttttatttat gtttcataat caatatggta aggatgcagc tccgtatggt
    30421 agaccgcctc cgaatattca atcatttccc caatttgcgt agacgatttc cgtatccgtt
    30481 tgatgccgat ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt aaaaatactg
    30541 ccgagaaatc atcagaaaat gagtcaatag tatatccttt tttcgtcagc aaacgataca
    30601 aagattcttt gccaaattct tctctgctga attgcatcat ttcttgtgga agataatgaa
    30661 caaaataaat ataaggctga ttgttcaaat aatataagcg cttaaaacaa acagcttgct
    30721 caccgaaaaa ggaaaacaaa ggatcttcct tctttatatg gaccaattgc aaatccagcg
    30781 tttctttccg gacttcatat cgggagttct ccaagatttg cgtaaagctt gccgctttgg
    30841 ataatcgatt gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt ttctttgcga
    30901 catattcatc tgatgccagc agttcgattg cctttcggac agtgattttg ctgacttgaa
    30961 acagttcttc taattcactt tctgtcggca acatcgaacc gactggatat tttcctgaga
    31021 aaatatcttc tttaatttta tctgcgactt ccaagtatag tacacgtgtc cttttcataa
    31081 cgtcaccctt tgctttacta ttactaaaag aatagcacat aaaaaataaa aaaagaacac
    31141 aaatcacatg tgagaagtgt gttctttttc attcagctgc tttaattaca agtttacgcc
    31201 atttgaggca attacttctt tataccaatc aaaagatttt ttctttgatc gattcagtgt
    31261 cccatttcct tcatcgtcta gatccacata gataaagccg taacgtttac tcatttctcc
    31321 tgttgatgca ctgacaagat cgatacatcc ccaaggcgta tagcccatca aatctacacc
    31381 gtctgctact gcattagcca tcgcttcgat atgacgacgt aaataatcga tccgataatc
    31441 atcaataatc gtgccatcct cttccatatg atcaatggct ccaagaccgt tttcaacaat
    31501 gaataatggt ttctggtaac gattgtataa ttcattcaat gcgattctca gcccttctgg
    31561 atcgatctgc cagccccatt cacttgcctc aagaaacgga ttttttactc ctcccagcaa
    31621 gttcccttct gaagttgctg cttgtggatc agtctcattg attgccgtag acatataata
    31681 actaaatccg atgtagtcca ctggatattc ttttagcaac tggaagtctt tttccgtttg
    31741 ctctaatttt tccggatcga ctccatattt tttatgggta cgcgctgtgt atgcagggta
    31801 ttctccacgt acttggacat ccgtgcagaa aaaattgaat tcttggtttt ggatcattgt
    31861 tgctgcctga ttgactggat tggcatcgat gctgtaagtt gttgcataga taatcatgca
    31921 gccgatttgg atatccgaac gcaattcgtg gccgattttt acggctttgc tacttgcaac
    31981 gaattgatta tgccatgctt ggaaaatatt ttggtactct cctgctccat tgctttttac
    32041 taatccttga cttaaagctg gaaagtgaaa ggccgaattg atttcattga aagtcatcca
    32101 atatttgact ttactgctgt atctctctaa tactgtctga gcaaatcgtt catagaagtc
    32161 gatcagtttt cgatttttcc agccaccgta ttcttttgcc aaatgaagag gcatttcgta
    32221 atgagaaatc gtgatcacag gttcaatatc atattttaag cactcatcaa tcagctgatc
    32281 ataaaattct aatcccgctt cgttaggagt accttcgtct ccatttggaa aaattcgtgt
    32341 ccaggcaatc gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg caagatcttc
    32401 tttgaaacga tcgtaatgat cgattcctcg atgattcggg taaatatatt tttcttgatc
    32461 aatcgtccag tcaaattctt cactgccgat aatcgcaaag cgctgcttac ctccaggcat
    32521 agcatctgca acggatagtc cttttccgtc tcgatcataa gccccttcta attgatttgg
    32581 cagcagttgc tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct
    32641 ccttatttta caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca
    32701 agtatatcgg caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca
    32761 gctgctctaa ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc
    32821 ccttgtttga ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata
    32881 tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa
    32941 atagtcgtga tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg
    33001 ataccttttc ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt
    33061 actttacccg ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca
    33121 ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat
    33181 gtacctacga atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt
    33241 gactctacac catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg
    33301 ctggagaaag cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata
    33361 caagcggcaa tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg
    33421 attccaaata acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt
    33481 gttcgcaatg ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt
    33541 ggcatcaata catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc
    33601 caatgcatgc cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg
    33661 attggactaa atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc
    33721 ccgattggtc ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga
    33781 actaaaatca tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt
    33841 tgaacccata cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga
    33901 atcactggga tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct
    33961 ggatacacaa gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta
    34021 gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac
    34081 aatatttgat aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag
    34141 aatcctttca ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag
    34201 ataatatcga tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact
    34261 gcaagacctt cctcatccac gatcgattga taaacatctg ctacattact gccaatcact
    34321 acttgatact gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt
    34381 tgcgtatctg ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact
    34441 aaacttttga cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga
    34501 ttatccattg ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa
    34561 aaacagtcac aaacattcgt gaacctattt tttcaatcca ggttttgcct gccatacagt
    34621 aacaatcctt atgattggat gctttttttc tcacttacta ctctttgaat atgaatcgtt
    34681 aaataaatct gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag atactcattg
    34741 atccgttcga cacaatgaaa cgcttttgga tactttcctt gaactaatgc gtagagaaat
    34801 tcatcttgct cgtcagatcc ttcccctttg agataacgct gggcaaaaaa ctgcagatga
    34861 gtaatgatac gctgataatt caaagattct tcatcgaaca cgatgccgaa gaatttactg
    34921 atgatactca gtatatctct taccattgtg gtagcggtca tcgtcacttg catgtcttcg
    34981 tttccttgtt ggctattcac aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt
    35041 tccacacccg tcttttgttt cacgaacaaa agagcctctt tcgctgcttg atattctttc
    35101 ggataaaatt tttttgtttc aaataataat ggattaggca gttggatatt ttcttttgct
    35161 cgtgtgatcg cataatgaag atgatcggtc aatgtcagat aaatattagg cgaaaattca
    35221 acttttagtt tatcttcagc cagttcgact acttgtttga cccattcgat ctcctctgcc
    35281 ggaatatcag ccatcagctg ctggaattgg tttttagcaa aagaatctct taaaacaaat
    35341 tctttttgga ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct
    35401 tttcccatac aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc
    35461 aaaattttat caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat
    35521 tcgcctactg tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc
    35581 ttattcaaaa tcatcatacc atcgaatttt tatttatgca agccttttca tataaaaaat
    35641 aaaaaaactg gaacacaagt ctatctagaa aacaaccaaa tcagagatgc acctctttgc
    35701 gtttctcttt ttttcagcaa attggttttt ccttatcgat tcatatcctt tggctgtttt
    35761 taatctgtaa cgacttatgt cccagtatcc taaaatgttt ttgtctctag attgaaagca
    35821 gatctcatat catctgtcgc atcttttatc gatgtaacac tttccagttc tccttgtaca
    35881 actagtcgcg tgacggcgtc catgtcgcca caagtaatta gtgtgacaat ttcttttccc
    35941 tcgacttcat tcagtaattg ggtatctgta ggagcaactt tttctttaaa aaatgttttg
    36001 tatgtataca cattttctaa atctgtcaaa tagatcttgg cacccatctc taaattttct
    36061 agcggtgtaa acagcaattc agggttcgtt gcacgatggc ttgctaaggc ataatttcct
    36121 tttcccattt cttgatctgg agataatgtt cctgctccgt aaagtagtga cgtattatcc
    36181 aagcctttga aaaccggtaa attgattgaa actgaaggga ctgcgatact agcaatcact
    36241 ggtagatctg ttcctctcat ttgcgacctc atgacccctt ctgtcgtcat tggttctacc
    36301 gcatcaaagt caaaactgac atctttatcg ttattctttt tcagatcttc ttttgttaca
    36361 ttcgctatag catatttatc tcctgtctct ctgacaaaat aatccttgat ctgttcattg
    36421 aaaatcaaag ctagtcctac taataacaag aggaacaaga ggatattgac tagccatctt
    36481 tttgcttttc cttttttttt cattttctct ccctccagac tcattttact gtttttcttt
    36541 tgctctttcc aacagccgaa tgacacattt ctacaactct gtgtattttt ttcgtacacc
    36601 tgcgcttttt tctacaggat atttcaaatt atttttgatc aatttttctt caatgatctc
    36661 atctagatcc aggtgaaggt tgtctgccat catgtatgaa tagatcaata catctgccag
    36721 ttcttctttg attcgttcta attgtgtttc ttttacttct tccggcgttt tccattgaaa
    36781 aagttctaac aattcagatg cttccaaaga aatagagata gccaaatctt tttcgttatg
    36841 aaattgtcgc caattacgtt catctctaaa ctggttgact ttttccatac tattcatttg
    36901 cttgccttct tccaatcact tcttacttga ttaatcatac cattttttta cataaaaaat
    36961 agatgatgct tttaaaaaaa ctcctgtttt cttttattta aaataaatgt catagaaaat
    37021 caataattta ggtagccacc atgtgtatgc agtagcgtgt cttcttccct tgaggctttt
    37081 tgtttttcga tacgttcttc ttcccaaaca gacaacgcta ctagaatctc tcttaatgat
    37141 tttccttctt cggtgagcga gtactctact ttaggcggga cttggttata ggcttttcga
    37201 tggatacttt catcttgttc taactctctt aactgctggg tcagcatttt ttttgtgatc
    37261 gtagggattt gcttgcttct ccacgcctaa aataagcgat attgctcttc cctttatctg
    37321 gtgtaagcaa agcagttagc attttgacga tcgtgggttc cgcttttcac ttcttatcta
    37381 cttagtatca tgaagtatac tttcgtacta atactaagta gcggaagtgt caactacttt
    37441 gtttaactac atagcaaaag aaacagaagg gtgacaacgg tcatcacgct tctgtttctt
    37501 ttgaatatgc tgtgttgttg tctctatctc cgtgtagact ttcctcaaac ttatcctttg
    37561 acagtaagtc agtaggctga tttgctcttt ctagatgcca gatacatttc tacaaaaaaa
    37621 tagtgattct gttttgaaaa ttcgattttt agttctgcat tttcgtaaac atccaacatt
    37681 tttttcgcac tgtatagacc taacccgcca ttttttttag aggtttttcc tcttttgacc
    37741 atctccgata gcgtcaaatt cgttggattt gccttgttcg ctatactaaa acgtgatccc
    37801 tctttttctt ccagcagatt gatcgtaatc ggactctgat cggctacacg atgatcgaat
    37861 gcgttagaaa taaaaataga taaacagcga ataaaaacaa ccagatcaat cgggattgtt
    37921 ttcacttcga caggaataac tacttggatc gggattttct ccgattctgc tttttctaga
    37981 aattttaaaa agacacccct gacagctggg gatacaatat ttgttagctg ttgtatttgc
    38041 ggtttttcaa aaatagctgc agaatgatct tctaatattt ctaaaaattc caacgtttct
    38101 tttttatttt cctgaagagt aatttttaat gaaaacagta gacttttata atcatgtttg
    38161 aattcctcta gatgatggta atattcttca tttatttgat acagttgaag cgattcctga
    38221 tgttgtcttt cttttacttg gaaactgatc aacataatgg aaaaaaacaa agaaaaaaaa
    38281 ctgatgacta tcgacaaaag agatagaagc agataattag atgagcggaa gtgaacactt
    38341 cccaatacca agtaaatcac gccccagctt aataaaatac aaatacctcc aaaatagtgt
    38401 tcagttggat aagtaaaaag caagttgaga aatctcgtct gtttgtctat actttttact
    38461 aatagataga aaataaatgc gataagtagt gcaaaaaaac ttgctgttcc aagatgtctc
    38521 tttcctaaat aatccatcag atcaaaaacc agaaaaaagc tgagtccagt gatagcaaaa
    38581 aaaagattac tgatcaaaca gctgataaac aggtttttcg tagtaaaata aaaaagaaac
    38641 atcaataata agccaactag gtaaggaaag ctcactttag ggataggaag taaaagtgtc
    38701 accaccatca aagctataca accaaagctg ttttttacta gcagttgttt cgaaaagaat
    38761 aaatagatac ctaaatctaa aatcaaagta aaggtcaaat aaagtgtcac tgttgcgacc
    38821 atcttattcc tccattgttt tgtttcaaaa tgtacacata tttttttaat aggagcctgc
    38881 atatgaaaaa gtattttatc atagaagata accagtttca cagcgaatgg atcatcagag
    38941 aattggtcaa tcatcatatc gagaatatcg tgcagctcac ttctatctct caaatcaatc
    39001 agttgatcca gttgggcact attgataatc acgatgtttt ctttatcgat atcgacttga
    39061 agcaatcact cacaggtatt gatgtcgccg aacaaatacg taaaaaaaat gcacatagta
    39121 atattgtttt tttcaccgct tatacgaatt ttgcagtaga gatcatcaat cggaaaatcc
    39181 ttccgattgg atatatcaga aaagacagga atttatctga acaaattcgt caagtgctag
    39241 aacagatcaa ttggaaagaa cagcagatcg taaatgtcga taaaatcttt attgatcaaa
    39301 aaggtggaag tctcgtcctg tatcctcggg aaattttata tattgaaagt atcaaaggtt
    39361 tgaaaaacca agttatggtc aaaacaacta cagaagataa attagtgaat acttctttaa
    39421 aacaattgaa gacagagtta gaaaagtatg cttacttttt acttttaaaa tcttactgta
    39481 tcaacttatc gcaattgcag aaaattgatt ctgctcatta tgtgttagaa tttttcaacg
    39541 gtgactcctt actcgttggt cgaaagatct ttgaaaagac aaaggaacgt ttccatgact
    39601 tccaaaaaac tgcttcgagt tgatgaataa gagctgagac gtaagcatag cagatttcag
    39661 ggagtgggcc ttgaatcggc tccactccct gaaatcgttg atatgatttt acgcttcctc
    39721 ttctgtagga gcatctgtta acgaccatgt gaatgttgaa acgtagttgt ctacagcata
    39781 attggataca ggaacaaata attccactga ttgtctccct tgttccacat tctctcctaa
    39841 tgaataaatc catgtgccaa tcccttcatt tgctcctgca gttactagat ctactgcctg
    39901 atctgctggg atttcgattc cttcagcgcc agttccagaa acggtagatg gcattccact
    39961 gctattagtt gcgctatttc gaatcacggc gtttttgaat ttcacctttg aaccagttag
    40021 tttattcgta tcagatgttg taccaataaa ttctgttcgt ttgactgata atttccaacc
    40081 ggcataattt ccgcttttat ccgttacttg tacgaaattc tgtacttgtg tttgtgctcc
    40141 agtactgtcc gttccgcttt gtaatgcagc atatactgtt tttgctgtac ctaacttgat
    40201 tgttccaaaa tcaagatctg gagcttggtc aatcgtcagt aatccggatg ttccctgatt
    40261 tggttcgtca ggtgtaaaag gagtatcagg atcatttgga tcaacaggag gaactggaac
    40321 aacgtcttca ggatttgcag catctggttt ggtcacagaa atcgttcctt ctgtagtggc
    40381 tgagctctta ttctcagcaa aaacactttg tgtacataat aatagacttc cagctagaac
    40441 ggtacttaat gcaattgttt ttttcatttc tttttccttc tttcttttaa aataaataat
    40501 ccagcaaata agccaataag aatgactcca acaagtatca gccatcgcca gtcaaatgtt
    40561 tctggctgat tttttaaatc aatatccttt tcattcaatt ggtccgctgc atcatcagtt
    40621 atttcaaaat cggtatggag attccagcgt ttttctttcc aagcaccctt cacaaccgct
    40681 tgataatgtc ccggagctaa tttttttcct tctaatggga caagtaaatc aaaatttgta
    40741 ttgggtgcta catctatttt ttcttttttt attgaaaggt aaggctcttt tgtttctttt
    40801 ttgtagattt caaccgatgt agtcaagccg aacatataag aagcatttgg attttgaaga
    40861 ttcgccttaa tcacatttct tccatcgctt tgatccgcat aagctttttt taatttcaaa
    40921 tctggggaag actctttttc gctatttcaa atcaagattg ctttcacttg agaaaactga
    40981 ttgacgacac caactgtact tttgttagaa acgttctctt ttttttcttg aaacgtgatc
    41041 cctcctgcga ccacaccagc cagctctttt tctggcgctt tcagtgaaag tgtctcagtg
    41101 accgcacttt gaggaggaac agttatttct cccactgttt ttgctaagtt tttcatatca
    41161 taacttaatg tttcatcaaa tttacttttt gattctccat attcgatcac accgtttgaa
    41221 ttcgtcgtag cggagtggat caatgtctca actgttactg atttatctgt tcggttaaag
    41281 accgtgtagg ggatttcctg tgttttccca ggctccatcc ttacatttat atatgttttg
    41341 ttcttatcaa tctgattagc cggtaattca aaatctaccg caaaattgaa ttcggaggca
    41401 tggacaggtg tctgccaaag gatgaatcca aggagaagga tcataagtaa agagcagttg
    41461 tttcgatgac ttttattcca tattcgattc atcaatcatc ctccttgatc tttattctgt
    41521 cggcgcatct gctaatgtcc atgttatctc tgctgagtaa ttaccagcta tctgattttc
    41581 tttggttaag acaagtttaa acgcccgacc gttatttaaa tattctgaat aatctgcttg
    41641 cacattcgtt gaattttgtc ctctgacaat ttccagttct tcttcattca ctaattgata
    41701 ctcctctcct ttcttgaaat ataggacatc ctgcatagaa aatcctgcat catttgtgaa
    41761 aggctgagat aattttatcg ttaagatcca cggatttttc gtttctcgct gatcataaat
    41821 ccataatttt tcctgtatat ccatttggcc aaatgcttct tgtgtcaaac cagttgcttt
    41881 gatctgacca aaatcgagtt tggaaggggc aagaaatgag agaccacctt ctactacgat
    41941 cttcgtagca ggtgcaaaag gcggggcatc atgataaaca actggcgtat cacttggatt
    42001 gtgattccca atttcatcgt tggtacttgg cttgttctgc acacccttag cagtagcttc
    42061 tcctgcttgg tcgtttaata caatttctaa tacatctcct gcaaaaagtt gattttctgg
    42121 gatagaaaga acaaattccc ctgtatcatc aatagttgtt tctgacaaaa cagcttgatt
    42181 ttttttcaat gaaaccaagg ctcctgcttc acctgtacct gtgatcgtgc gagaagctgc
    42241 attgatcaat gtggtactta aattagcagg ttcaggagga atcacatcga ctgtcgtttt
    42301 ggcagagtcc agttctgtca cttcttggcc acctcttact tttttagcag caacaacttg
    42361 cacttgatcc tccgttttta atctctcatc attgggtact tgaacttcga acaagccgcc
    42421 ttgttcgcct tctccccatg ggttatatcc tgccccttca ttttcatctg tttgtccttg
    42481 cgtgagagca gtaatttcta cagatgattt cgtcgtatct gcgtaagtaa tacgtaaagt
    42541 cacttctact tcgcctgtcc aagcatctct tggctttgcc tcgacacctt cgggaacaac
    42601 gacatgccca tagatttttt tatctgcatt cgttggcact cttaaggatt caatcatcgg
    42661 gagctgatta ttggcactga ttcgattata atttttcatt ccggcacctg tctgagcaaa
    42721 ttcatttgtt aaacctgcac tagggcttga tacgaaattg cctaagtcgg tcccattgat
    42781 cgataagtct gtcatatcag aaaaataatc cgggtctttt tcaaagttat cgaatgttgc
    42841 gcctgttttt cgccataaag cgatttctga atgggcgatc tttaaagaag aatctccatt
    42901 tccttggaat acccagccgc ctatgccatt ggtctcgctt ccaatccgtt tgttttggaa
    42961 atcaaaatag gtaggagatt ctaagtccac attcaacaca tccccactga tgatgccggt
    43021 cttatcttcc cctgttttcc cgaccatgac taaataagtt ttttcacctg ccgtaaaatt
    43081 taaatcaata tctccagttg tatctacagt tgctccggaa tctgcgcgta tatcgatatt
    43141 tgatttttct ccagtgactg taaaatcagc tgaacctgga ctttctagaa aaccagaagc
    43201 gtcataaaat tggatcgctt gatttccttg attgagtctg ttcacgtaag atttgccatc
    43261 acctgtattt cttaccctca atgagcctcc attggttact gtcagctttt gcgtaccatt
    43321 ctcaaaacga actgcgctgg aacgcccact ctgcttatcg atgtcgataa ctgatccggc
    43381 tccttctacg atcaaagaaa ggccttttcc tacaaaacgc atcccattgt tcccgggttg
    43441 atcgtcatct ccagccattt tgaccgagat ttgtccacca cttgatacat ggatttgact
    43501 agaatctcct tgcaagcgaa atccgcccgt agttgctgta ttgatggtga ttttcccacc
    43561 attcgataca gtgactttcg atcctgaacc tctcatttgg atgattccgc gattgttgtc
    43621 attggtaata ttgatctttg tatttaccga aaattcgctt cccgaaccac tgacttcaaa
    43681 catgacatta ccaggtagcg tatcgactgc ctctccttca tcgctaacgc catctgcccg
    43741 atcataatca agagagacct ttgaaccgct taagatatgg attcctggcg ctgtttttcc
    43801 actaccagtc tcatctcctg aatctagaaa accgtagaat ttccctagat acggtccata
    43861 aagttgacta ccattgatca cattcaattg atagcttcta caaaaatagg ctttacccgg
    43921 agcggcattc agtccttcct tcactggttg gacatccaac acaccggaaa caccatctaa
    43981 tgtgacagtt gcacgaggag cgtaaatcag tcctaatttg gacccatcat acaaatcaag
    44041 tgtcccggta aattttattt ctcccgtaaa atgatatctg cttccccaaa aatcggtcat
    44101 ataagcaatg ataatggcat ttccatcgat atttggtgtc aatccacctg gatgtgcagc
    44161 tgttccttta aaattctgga actcgatcga ctgaggtatc tcattgatat aaatatggtg
    44221 tgtgccgaaa ttgtgagcga atccaccaaa atcaataacg atatctcgtt tgatctctaa
    44281 ttccctgtct aaagtcaaag gctgaatcag tcggatctta gttactgatt cgtctgcgag
    44341 aagctgcgcc agttcttctg ttgtggcagc ttcgctgtac ccatccgctg aaacagctct
    44401 tgccagtgcc gcttcactgc tatcagacgt ttgttcagct acttctgagg tctcacttgt
    44461 cttattttct tcttgccttt tattagtaga agcctcactt tgagattctg tagtttcaga
    44521 agtaatcgat tcttctgaaa aagtagtctt gtcaaaactt tgattctctg attgcgttga
    44581 ttcgtttgtt ttctctacat cggcaaaaac ggatgtcacc atcaagagag ggaagacaaa
    44641 caaaatcgac gcaatcagct tttttacaca ttgattcatt ctccaccatc ctctctgacg
    44701 ttttgacaat tttattttat cgttttaatc tatttccaat tgtactttca tatttaaaat
    44761 atgtattttt atggaaaaat ttttctatta ggtgtaaatt tcttttttgt acaaaaaaaa
    44821 ggctgtgaca aaagtcttgt cacagtccct tatcccgaac aagcggtgtt ctcccgccaa
    44881 ctcctcggtc acaacctcca ttttaacaat aaacctgtaa atgtgagggc aatactttga
    44941 ttgtcacagg taatttatcc ccttcatctc catcaacatt cgtcgtcaat tcttcctcat
    45001 cagctaatga cacaactaat tgctcacaag taaggtaggc tagatttttc gtagattcat
    45061 ctactccttt taataggtct ggcaatgttt taagcgtatc gatcaaagaa gaatctttta
    45121 aataaaccaa atggagctta ccatcatcca cttttgcatt tggaagtaat gtttcaaaac
    45181 cgccgactga gttagttagt cctatcagca aggtactgct ctcaatttct tccttctttc
    45241 catcaacttc tacgtgaaac gagtaggatt gtgtggaagc taactgtttg attcctgaca
    45301 tgaaataagc tagttttcca aatttcgtct tcttctccgc atctacatca ttgattgatt
    45361 cagggataga gccaattgcg acgacattca taaaatagtc gtcatttatt tttccaatat
    45421 ccaatggctt gactgattca atcgaaaaat gattgatcgc ttcctcaggt tctagcggta
    45481 tccctaatgc tcgagccaag tcattcaccg tacccaatgg gaaaaatccg aaatttggcc
    45541 gatgttcttg ttcagcgatt ccactgattc cttcgttgac tgttccatct ccgcccatga
    45601 caaacacact atgataccct tccgtagccg cttcgcgagt aaagtttttt gcatcccctg
    45661 ctttttttgt atgcaagacg accacttcat caaacaccga ttccaatttt gctatcgcta
    45721 attgctcaaa ttcctttgct tgttcaccgc ctgaactggg gtttaccact aataaaactt
    45781 ttttcatcac tttctttccc ttctgattga tttctttaat ataacgatag cataaagaaa
    45841 aaaattaggc tgtgatgaag tactgttcat gaaacattca tttatttttt gacaagataa
    45901 aacatccata ctatgaaact gaggaaaatt aaaacaatga tataggaaat aaaagaaaat
    45961 gataggcatt gtatacctaa taaagtaaaa atcttaggtg gcagataaaa acacaaaaat
    46021 agcaagagaa tgtaatagcg tttcttccac tttttgttat cagaccttat agcgatcaga
    46081 ggaataccaa atgcataact gaaaaactct tgatgtactg ttaatatgtc acggccttga
    46141 ataataaatg cgacaatccc cgctataatt agtgtgccta acaccaaaac ggaaaattga
    46201 tgaatatatt ttatatacaa atgatccatt gtacttcctg cctttttatt tgtgagaaaa
    46261 acaaatccgt atacgtgaat aacttctaga aataaaagaa atttgcaaaa ccactccatt
    46321 tatctacttc tacgtgcatt ttttgatatt gttttcttta tttaagatta tacagaaaaa
    46381 tatctagggt cgctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa
    46441 gtagtaatta aactactgtt gatttagttc taataaaaag atttagatga acagttttat
    46501 tgatttaaat agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa
    46561 tctgaagtga atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg
    46621 tctttcaata cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct
    46681 gttgcaattt ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt
    46741 ttcaccattt taaccacata atattccgta gtataagttg aagcattggt agaagtgatc
    46801 acatcagaaa cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc
    46861 gttgtagtag tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt
    46921 gacttgtctt tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct
    46981 tttgcatcat cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat
    47041 tcccaagccg tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga
    47101 aaggctagac tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc
    47161 aattgtgaat caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt
    47221 tctttggttg atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt
    47281 tgcacagcgg aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg
    47341 attgtaccgc ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat
    47401 aagccagtgc ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc
    47461 tacttttcgt tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga
    47521 acacctttac taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa
    47581 gttcgctcag cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg
    47641 ttaccgtttt tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa
    47701 tgttatcgat tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg
    47761 ttcgattttc cagacatttt tttgttgttt ttattatgat atatttttat acaactaaaa
    47821 atgaatatat taaaactacc aaaaataact tttaacgctg tttcattata tgttttttct
    47881 attttatata aacttttgga tacttaatac caaccggttg gattgtacca acatgagaat
    47941 gtgtcagttc tatttcctgt cgcacttgca taggcccata ctccttttgt tgcatttgct
    48001 gatcgaatag agtttccaac tttcgctttt gcaccgtgac gattatagac acgactatag
    48061 tggttagaat gacctgtttt tctcgtacaa gttgtatcac tataataaac ccatcttcca
    48121 gagctatgat taattgctgt tctagaaatt tcccggtcta ctggtaaatt tgacttaata
    48181 atattacctt tagcatcaaa atgaatctct cctggcttta aagatttgta atagatttca
    48241 ccgtgaactg aatttgcttc cacttcagat gacatagcgt ttcctccaac aaaacctatc
    48301 cctagtgcac aaataataac tgcttttttc ataaaacgac atcctttcct ttttgatttt
    48361 taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gactacgata
    48421 actaacgtac cttgggaagc ttgttgcgat aagatttcaa ctactttatc cttatttctt
    48481 ttatccaatg acccagtcgg ttcatcggct aaaatgattt tgctattctt caataagact
    48541 ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc
    48601 ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca
    48661 tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc
    48721 aacccaaaat cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg cttctctctc
    48781 ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact gaactgatca
    48841 ttcaatccta atatattcaa taacgtactt ttacctgctc cactttctcc aacaacagtc
    48901 acaagatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg
    48961 atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc
    49021 aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg
    49081 aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag
    49141 attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca
    49201 aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat
    49261 atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt
    49321 atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac
    49381 ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt
    49441 tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata
    49501 tttctttcca aatacatact gtttttcaat gtcagtattt caacaaccgg ataatgttcg
    49561 atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat
    49621 ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt
    49681 aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt
    49741 atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt
    49801 atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact
    49861 aatatacttc cgtttttatt caaagtttca tataattcct gatctgctgc tgatgcaaaa
    49921 cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga
    49981 tagtcctcac cagcgatttc ttttggtggt tcatgggaaa cagctaactg tgaagaagaa
    50041 aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca
    50101 aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca
    50161 actaataaaa gagaaatcaa agagatacca tatattccgc tgaacaagaa aacgtacatc
    50221 tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct
    50281 gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt
    50341 aatgaaaagt cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat
    50401 gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg
    50461 tctgtatttg aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg
    50521 gcataatcag tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat gacccgttca
    50581 taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc
    50641 gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa
    50701 gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc
    50761 caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt
    50821 tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa
    50881 tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga
    50941 tagagtacct gtttatttat actaaccaag gcaaaaccac tcatgatacc ggtcactatg
    51001 attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat
    51061 tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaagt gtataggtca
    51121 tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca ccggagaaaa
    51181 aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaattgg
    51241 tgtttccgat aaaacgattt ctagttggga aaattctcgt acaatgccag accttgaaat
    51301 gctgtcactt ttacatcata ctttaggtct gccgattgat tttccttcta ttgaatcagc
    51361 taccgaatca atagaaccaa cattgccaga tgaacctctt gttaacaaga aatcttattc
    51421 atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt
    51481 ggtctatctt tttcttatca attatcaata caaaaatgaa acgatcgatc gattcaatcc
    51541 ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg acaattccat
    51601 cgaacgttgg gtatgtaatg atatctttgg ccatggtaca tggctgtctt ttcacgctgg
    51661 agaggtagaa aaagagaaaa accatgctgt aatcaaacac aaaggaaaac acgtcatgaa
    51721 cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc tcagccaaga
    51781 ctatgacgac gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga
    51841 acgtagagat agaaaaaatc aatgcacatg atcttatcca aaaaacaggg gtacaaatta
    51901 attgaaaaag ccttgagatg aaagttccta aataaaaaag ttcagaatga taggtatttc
    51961 tggcttatag tataatattt acgccacagc ctcccaatga ttctttttgg aatcataatc
    52021 aaggatggac attcacaaaa atattttgct cattcgatcc gaatagatta acgaccaatc
    52081 tagtaagtaa tatagattgg tacgtagtca agctgcacat aatttagatc aattttagtt
    52141 cttttttata tttctatgat ctgctcattt acttttatat gatggcttac agtaacataa
    52201 ataaatatga taatcgctgg tagtttatcc aagagtactc aataaaatac aaaatggatt
    52261 gttcaagcgc ctatcataat gataaaatga aacaaataaa cgatacaagg aggaagagac
    52321 tttgagtaat ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt aatctatggg
    52381 atttcggtag catgggcatt tttttataga ggaatcacac caacctggcc gttttatctc
    52441 ttgttagtgg gaatattagt aggaaatttt gcccctcttc actatcgtac gaactttttt
    52501 ccaactgatt ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa cattattgaa
    52561 atgacaatta cctgtataat cattttctta gttatattcc taaacgtctt ctaataagaa
    52621 tctcttaaaa ttagcttgtt acagttagtt acctagttta tataacacag ttatcttatc
    52681 ttccttatca acatcttttg ccaactcttt gtcttaaatt cgaaacatat aagcagagct
    52741 acgaacacca agactgtttc ggctctgttt tttgcatcaa attcactctt atatagctga
    52801 taatctcggc tctttaaaag aaattatttt cgcttcattt tattataaaa aggctaggaa
    52861 ccttgatgtt atcaggttcc tagcctttat ttaagtatta ccccacagac ccttccatat
    52921 agatttttga attacacact agtttaaact aaaacaaaca aggaaaaact taccatttaa
    52981 tcataaaatt agctcatatg aatatattag aatgtaaaat agactggtca aaatctggtc
    53041 aaaaaagagg actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt
    53101 tttattggat tattcccttg ttcgatgtag actcaaatca atctctttac ttgctaacat
    53161 cctttatttt accttgacct gttgataata gacagttaaa tcttgattta aagtggggca
    53221 attaatcaag aaaacagggt tctaagcctc atttgttagc ttgttctctt agtaatattc
    53281 ctcgtgaaga atcgggacga ttgtttggtc aataaacaat atctcacttg catcgaaaga
    53341 atatttcttg aacaactgca tatcaacttc ttcaatacct acattccagt taatgtctga
    53401 acctgaggta aaatcttgta ctggtatgaa catgaactta tctttggata ggtgaataga
    53461 tgtcactgct tgaagtaata aaaatctgta aaaacgtgtt cgtaaatact tcagataatt
    53521 aattgcttcg gtttcggtat cagcagaaaa tagcactagg tatgaatctg taactacttc
    53581 attaggttta ataactctgt tagtagaaat caccttaaac ataccatttt tgttcggttc
    53641 acctgcatgt tcgctagtca gtgagctact aatgacttta tactttccaa ccatatcctg
    53701 tcctttagtg atatcttcca gtggaacaaa gcctacccca ccactagaga ttacttttat
    53761 tgaatttctg gaatcctgca cttcttcccc tcgaattttc gttggaagcc caaatgggtt
    53821 acgtgatata acaaagtcac caacactagc gtgttcccca attttgtgaa ttgcagaaat
    53881 ggcttcatta tatcgcacaa acactggaaa ctcgttcatt tttcgctctt gtgtatttga
    53941 tacaccatca tgttgattag tatactgtgt caatccatta tatttcttat cccacaagaa
    54001 atagctaaca ccgccaccaa tactggtgtt tgggaatatt tctttcccat ttactatatc
    54061 caccatcacc ttcaagctag agctgttaag cataaagtca cggtacttat ctaacccttt
    54121 accacctgca aaccacttcg aaggagtaat catcgaaaca tagcgttctg ataacttaac
    54181 agaggtttct acaaatagat tatatagcgg tgtagcacta gctcctgaac ctccatcttt
    54241 ctcctgataa ggtgggttac caattatcac atcaaatttc acgtttttat aaactccttt
    54301 caaattcgtt aaaccgtcat tgattgaacg tttcatactc gtaactaatt cacttgagta
    54361 ttcaacattt accttccatg cgttataacc aatcaaagtc ctcttcgtta tcgtcttagc
    54421 cattggagtt ttagaaatga caaaaatttg ctctgataat actttttccc atattgcctc
    54481 tgaatctacc cttccagcat taatatcgct ttgtttaagg agctgaacat agtacatact
    54541 cgttgctgca tataaaggat atagccctgt ttttgagttt atctcaagaa tctttctatt
    54601 ttccttaaaa atatcatctg tacagtcagt tttcctccat cgcacgattg gttgaccgtt
    54661 ctccgtagaa ttttggtatt cttcatcaaa gaaggaaagt ccaccgatag tgttaccaag
    54721 gtgcatatta acaactcgcc atggagtcaa tacggtttcc ttgtctggat ttttaaaagt
    54781 gctaaataat tcacctattt tcctagtacg ttcaataaca tctaactcat cataagactt
    54841 tgcacgtcta cggattatgc gtcctgcctc cacaaaaacg tctgcatcat agtactcaat
    54901 aaactctctg aatcgccctt tacttaaccc ctctggcatg aactcattcc atgaagcctc
    54961 atcaaccaaa ttaagaaaca tctcaatagt aacgtcatct ttaatatcaa tgtccatccc
    55021 ataaatcatc ataggtatgc gaatagaaac gcccctcaaa atggaaatca ttttatttcg
    55081 ctgctctcga agcctctttg ccctatctat tgcttcctgt tcctctttgg tacgttgacg
    55141 ttttggtttc ttttctgccc tcgaagcttt ttcatactcc tcttcactca taccgttatc
    55201 tgaaacaatg aagtctttca ctttggttgg tttacttgag ccaacaattt ttttaaggtc
    55261 ttcaaatttg gtaagatcag cttggtctaa atttaatagg ttgtcattgt aaagagagct
    55321 gtcttcaaag ccagtccgta cagctttttc tgcataagcc ttcttcagtg ccgtaagcat
    55381 tcggtcaacg ctaaattctt tcatcacatt cccctgttta ccaaggatag gtaagaaatt
    55441 tagcatgcgc tctagttttt tcttttgctc tgaactatta acctttccct tcttgctagt
    55501 tagtccgaca cttccactca taatttttaa agcacggtca ggtgcaaaat caaagatgta
    55561 gcagtttttc ttaattccaa gctctgcatg gttaaaaggt gtctgtgcac ggaacgctgc
    55621 ctgcagataa gatgtaggac tttctgtgtt tgaaaggaaa aatacccctg tccactctgg
    55681 aacattaact cctgttgtaa gcttgcgaac tgttaaggtt atcgtcttag acttagctgg
    55741 attaccaata gctttacgca ccaattctaa atctgaatca ctagcatgct cactgtcacc
    55801 tgtcttgact acgtttgcaa tattgtaatc tttgaatata gggtgcgttt cgagcactct
    55861 ttcaaaagca tttgcctctt taacccccgg catcagccat aaagtatgcc gtaagttttc
    55921 tctaaactct ggttttgaga aaggaaaatt agtcttttca tcgtttgtag taatataatc
    55981 aagaaacttg cgtacgtcac cttcatgaag caactttgtt tcatcatctt ccttaaccct
    56041 aaagaattct cggaagttaa atgccttatt ttcatcgaaa taatcaaatt tatttggtat
    56101 ttcaaaggtg aacatagata ctttcggtaa accttcgtat gggttaggtt cattgggatg
    56161 ctctactgac catttctcct tagcttcttg ctccatagta taatcccaag tgaaaatatt
    56221 atcctcatca tacttatcga aaaggttaaa tggtgtaccc gaaagctcta agaccttggt
    56281 atgctctttc cgcaacttag cctttacgtt attagctaat tccgtttcgt ttccttcgtg
    56341 agcttcatca ataataataa aatcccagtc aatcgctaag aattcatagt ttttatcccc
    56401 tgcttttcca cctgcccact cagaaccgcg taagtcctga atacttgcaa aatagatgaa
    56461 aggtttttcg gtatcaacaa ggttttgaat cttttctcca tcttgttttg aaccatagat
    56521 gtacgaacca tctgtgaaga tttttttgaa atccgtaaac caaccatcac ttacaacagg
    56581 tcgatgagtc atgataagaa cttttttgta ttggctctct ttaatcactt gaagagaaga
    56641 aagcgtctta ccaaaacgca tcttggcatt ccaaagcatc tcatctttcg ttttgaatac
    56701 agtcttagtc tgctcaatag catctttttg ttctggtcta aaattaattt tgaactcgtc
    56761 aaagtcatct ttttctgagt caagactacc tttaccatct ttaaccgctt ggattgcttt
    56821 cttggcaacg tcaactgtta cactgaacca ctcattaggc ttccctgtgt catcttcgcc
    56881 aatcgtttga ggattatagc ccgaacgctt taatacttca tgcacgtcat ggtcacggaa
    56941 aaattggttc ttcttattaa cagccaattg cgcccaatga aggacaaaag gtacccccgc
    57001 tgttgtcatg tactgcttta ttcttctact tgcatactct cttaaatact cgctattagg
    57061 agtaaagtct gcctcataat tctctggtac tgatgcatcc ccaatctttt ccagtccttg
    57121 gtacattttc catgtgccgt cagcatcctt ggcactctgg atgtaaataa gtttacggtc
    57181 aaaggtgtta taggtgataa caaacatctc atccgtaatt tcttcctttg ggtttaacga
    57241 tttatcccca gacagattgc gtgcaaagac tcttagcaag gtatcaagag taactaaaga
    57301 ttcgaccgcc tctgaaacag gtataattcc attttcatgt gctgcaacat ttccatattt
    57361 tctcaactta tgaaataagt cagctatcgt aggagtaata ttatttctga ccctaagctc
    57421 gcgaagtaca tcaacatact tcatactttg ctggtcaata cccacatttg aagcaatctt
    57481 ccgtgctgct acctccgaat aacgtctagt tccaatagca acttcattag gtatttgatt
    57541 aacggttaca cggtacgtgt taattgcatc tactaaatta ggaatcccaa ggaagtgttc
    57601 tagcttagaa ccatatttag gtaagtaatc gaagtattta gctttagatt tcaacgctgt
    57661 cgtcatcaat ttcctccatc tcttccttat aaacctcagt tatcggcatc gctacataac
    57721 gtaatattgg atcatttgaa acttcttcat cgacctcgtc atcaaatgaa aacaaatcga
    57781 gctgttcaaa tacaggcaga gctgagtatg cctctccaaa ctctcgtgtt acccctgaat
    57841 aaatatcctc caaagaatat tcagttcgat ttacagtgat ttttttctgt ccttttctta
    57901 gcgtcttagg tgtccactca ctaaaaataa tcggattatc atctggtgtt cgttttgtaa
    57961 gaaaatctcc ctgtttgata ttcctagaaa tcaaagtctt tgcactgtct tggactttcc
    58021 ttttcattgc ggtaccatgt tttttcgatt gttctctgta caaatcgttg aatacttgaa
    58081 acatgttcat tacacaggtc tgagcattgt cttcgagtaa ctccacgcca tatagagttg
    58141 aaagcgccag taaggagtaa ttttcatact gttctaggga ctcattatat tcacgagcaa
    58201 ccattgccat ttttctagtc aaaacctcaa ctaagaaagc accttctcca gctgaaggct
    58261 ctaagaaagt cgtctgcagc tcctcgcaag cctcttttat gcctggcaaa tccaacatat
    58321 ctttgacagt tctttgcggt gtaaatacct caccgtgctt ctggacacgt tttttacttt
    58381 tgattagtcg tccttgttcc tcttcaacca tcaccatatc ttcaaccact aaaaatcacc
    58441 catcttccca acacaaataa ccgaaacgga tgctacgaat cggttatcac tttcatatat
    58501 gggcttttgg gcagactgct ggctagtact gtgattgcgt gtcatcatgc ccttttcctc
    58561 cttacaagtc aagattttta cttgaaaaaa ctcgacaatt tctaattatg cttatgttca
    58621 attttaacac atttttgttt tttcctctat ctcaaaataa agaacaaaat atagcttaac
    58681 tggagaatta tagagtttgc ctcttgcaac acaggggagt tagcgaagat gttcgtctgt
    58741 cctattttat ttttatgaac atgaaaaaag accaccaaac taggcagtct ttgacttatt
    58801 attatttact tttagctatt cccattaaat tttaccactc gtcccttttt cgccctcttt
    58861 agatttagcc gagggtcgtg tggtaaaagc tcattattgt cttaacgtgg tcaaaaacct
    58921 ctttcctacg tttatttgat aacccatact gacgaaacac ctctttcatt atcgcaatca
    58981 tttgctcatt agataacttt tcatcatacc catcaatgaa gttctctaat gactgactat
    59041 ctaaagcttt ctttatttca aagaacaagt cataaacagg tttatcttgc ttcttcagcc
    59101 agtgaacata gccctccctg tgctcttttt cccgttcacc tgttactcct tgctgtttag
    59161 gtaatcacag aatgttgacc gactgacttc tatacctgta tcttcttata ttctcgataa
    59221 agacttctta atggtgagtc cttcttgata atacttcagc ccagcctgta aaacttcgtc
    59281 tggaaacctc tttcgatgtt ctgtcttgtt cgctgaaaaa ctcttgtact catcatcaaa
    59341 gctgagtgta aataccacaa caagatgttc gtacgatagt ctgaccagaa aactgacttc
    59401 cttgtcgttt gtacccaaat tttcatatga agtagataaa agaactgtat acctggaaaa
    59461 gacgtcttct tttacgaagt caatgcacaa tagacgtaga ataggctatc ttcttcgaca
    59521 gttagtgaac ctatatcgtc tgctaatagg atatccgact ttaaatctaa atatttttcc
    59581 tctgattttt taatttccct cttgaaataa tctattgtaa tttgtttatc tgaattgttt
    59641 gcaattaacc cctgcagaac ttctctagct tgttcaacat cccgtattac catggaaatc
    59701 actaggtatc aaactgagtc atacgaagac aaaaacataa aaattgacct gttaaagggt
    59761 cactgaataa catataaaat caataaaacg gagtcagaca cggagtcaaa aaacgctgag
    59821 agtaaaaaat atacagtccc ataggtaaag tgccacagga ctgtatattt ttttatttca
    59881 gattacctga aatgattcca tttacttttt atctcatttt tccctactag ttagtatcaa
    59941 atactctcct attttctcaa taaactattt tgggttacat ggtatctgaa acagctaaaa
    60001 ccttgtcatt acagcaaaaa atcactttat ctccgtttca tttcagatac atttcactta
    60061 agcggattcc caaaaaataa aagaaaaaga aacccctagt tagcctatgc tttctaatga
    60121 ttcctctcga aacacggtca aaaaacaacg gggtagggta gttaataaaa agatagaaaa
    60181 accattattc aatgactttc tcaaatattt cgttgtaatc agttaccttt ccgttcttaa
    60241 ataccacaga ataatctttt tttccatttt caagtaacct aagtggaata tctttaccga
    60301 cttgctctga caccgcatta ctgactcctg ttagattagc taataaatct gataaagatt
    60361 gagcatcagt cggaatctct gtatcagtat ttatcgttat tcccttatct tcatttacaa
    60421 gtttccaagt aggtggtata gccgcgtatg tttttatgaa cgtatcaatt tttttatcta
    60481 cagtttgtct tgatattgta tcgtttttct tttctacctt accaattgga ctctcatctt
    60541 tcgtagtcct ataaagagtg gctgttgata agtgttcatc caaatttacc aacacagtta
    60601 atttatcttt ttcaccgtca ataggcatga acgttatatt gcttattccc gtggtttcct
    60661 ttaattctgg aatcgcttta actaattcat cttttgatat catgattgaa cgaaatgtag
    60721 ttgaatcaaa ttttttatgt cttagagtat acttaaaatc caccatttca ccattttttt
    60781 gtagttctat tactctgtct ttagttattt taaaattata gtctacagga attttctcag
    60841 cagttcctga ccaatatcca tagaaatctt tatctttatt tctacaacct gataattgaa
    60901 taaccagtat agttcctata accaaaaata tacctgtttt taaataattc tttttcatca
    60961 taaagctcct cttttaaagc atttaatcaa caatgtaaga acccaatttt taaagctctt
    61021 taaaaacaat ttccatcaat tattataaca ttatttttct catctatatt agtattttat
    61081 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttggac
    61141 ggtatacata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
    61201 atatacacgg tctttctttg gatagtactc gacatttata ttgatagcac ttccattgtc
    61261 taacgtagct ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc
    61321 acatgaccta tttaagttgt actgttcttg taaaatctgt gtcaccaatg gtttagatga
    61381 cttctctaat ccagatgtac ctgtcatttt tgaaaaaatg atacctataa tagccaagac
    61441 aacgatacct ataatgacaa acttcttgtt catatttact ttattcaact ttttatctcc
    61501 tatctccaaa ataatttgct cttatccctc ttatcagtac attgacatcc ctaaaagtac
    61561 accaaagatt gctccagcta cccctgctat catcaaaatc gtacccattt tctcatcttt
    61621 ttctcgatat aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc
    61681 taacgatact accatagaaa tccaaccaat aatcaaatac agcttccctg tctcttgtcc
    61741 atgtacgatt tgttccttca tttcactaaa tgtctgctca gtacttttct ttacttcgtc
    61801 tgctccagtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat acttactttc
    61861 atctgttatt tctttaccac attttctaca atacatgtct tctcctccta aaatcattca
    61921 attgcttaat agttaactgt ttactttatt aaacacatca gaaaacagct cgtttattgg
    61981 tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg ttctccctgt
    62041 ttgccacgaa cttacacatt gctgtgtaac attcattttt tttgctaact ccgtttgaga
    62101 aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc
    62161 ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa aaacttcaaa
    62221 taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac
    62281 agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag
    62341 aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
    62401 cagagcctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
    62461 attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc
    62521 ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
    62581 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
    62641 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt
    62701 gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgataa
    62761 agaggaacta attcaacacc agatgctatc aattctttta ttacttcatc tatccctgct
    62821 ccttttgcta ggtagactaa aaaactactg cttgctttca cattgtatag tttcttaata
    62881 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttctttt
    62941 aaagcatttt ccacgctgtt attcccaaca ttacgtagac gttcacaaac agtgttaata
    63001 agttctttct ctgtcattag atatgtccct tgccacgata agtcctgtaa aacttcatgt
    63061 gcctcatcta aaatcaattg tttttccttg ctgagttctg accgaaaatt ttgaggaaat
    63121 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
    63181 tatgtaaaat gaactttttt cagaagtgga aggatatctt cattcaattt ttgaatcaca
    63241 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
    63301 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
    63361 tcatctaatt tttttatcaa tcctataaat gtcataaatg atagtaactt attccattta
    63421 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctagttg ttctcctgat
    63481 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaaggca
    63541 tcatagacac gtcgcatttc ctctagataa ttatttattg tcctgaaaag cttgtcttcc
    63601 atagattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
    63661 ggatacaggt ctagattctc taatagccaa ttaaatgttt ctacccgact atctttctct
    63721 agaattaaca tcacaacccc aataaaattt tcactgatac cacaattact ggaatgacaa
    63781 taaaaaatgg aatgcccagt tttactggta aaaatactag cagatggatt atggtcgtcg
    63841 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
    63901 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
    63961 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc agtttcattg
    64021 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
    64081 tcaatcggaa gctcagtaat atctaacatg ttgtcttc
//
LOCUS       DACDBG010000007        60039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDBG010000007 DACDBG010000000
VERSION     DACDBG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.007"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(66..1022)
                     /locus_tag="IVZ90_000420"
     CDS             complement(66..1022)
                     /locus_tag="IVZ90_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8261495.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1193..1561
                     /locus_tag="IVZ90_000421"
     CDS             1193..1561
                     /locus_tag="IVZ90_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8261496.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1731..2162
                     /gene="mraZ"
                     /locus_tag="IVZ90_000422"
     CDS             1731..2162
                     /gene="mraZ"
                     /locus_tag="IVZ90_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8261497.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2183..3142
                     /gene="rsmH"
                     /locus_tag="IVZ90_000423"
     CDS             2183..3142
                     /gene="rsmH"
                     /locus_tag="IVZ90_000423"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8261498.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3147..3548
                     /gene="ftsL"
                     /locus_tag="IVZ90_000424"
     CDS             3147..3548
                     /gene="ftsL"
                     /locus_tag="IVZ90_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8261499.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3549..5741
                     /locus_tag="IVZ90_000425"
     CDS             3549..5741
                     /locus_tag="IVZ90_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8261500.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5767..6729
                     /locus_tag="IVZ90_000426"
     CDS             5767..6729
                     /locus_tag="IVZ90_000426"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8261501.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6743..8104
                     /locus_tag="IVZ90_000427"
     CDS             6743..8104
                     /locus_tag="IVZ90_000427"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8261502.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8130..9218
                     /gene="murG"
                     /locus_tag="IVZ90_000428"
     CDS             8130..9218
                     /gene="murG"
                     /locus_tag="IVZ90_000428"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8261503.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9233..10453
                     /locus_tag="IVZ90_000429"
     CDS             9233..10453
                     /locus_tag="IVZ90_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8261504.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10609..11934
                     /gene="ftsA"
                     /locus_tag="IVZ90_000430"
     CDS             10609..11934
                     /gene="ftsA"
                     /locus_tag="IVZ90_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8261505.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11957..13198
                     /gene="ftsZ"
                     /locus_tag="IVZ90_000431"
     CDS             11957..13198
                     /gene="ftsZ"
                     /locus_tag="IVZ90_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8261506.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13215..13892
                     /locus_tag="IVZ90_000432"
     CDS             13215..13892
                     /locus_tag="IVZ90_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8261507.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13906..14505
                     /locus_tag="IVZ90_000433"
     CDS             13906..14505
                     /locus_tag="IVZ90_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8261508.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14520..14807
                     /locus_tag="IVZ90_000434"
     CDS             14520..14807
                     /locus_tag="IVZ90_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8261509.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14856..15638
                     /locus_tag="IVZ90_000435"
     CDS             14856..15638
                     /locus_tag="IVZ90_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8261510.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15659..16366
                     /locus_tag="IVZ90_000436"
     CDS             15659..16366
                     /locus_tag="IVZ90_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8261511.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16628..19426
                     /gene="ileS"
                     /locus_tag="IVZ90_000437"
     CDS             16628..19426
                     /gene="ileS"
                     /locus_tag="IVZ90_000437"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8261512.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19475..21001)
                     /locus_tag="IVZ90_000438"
     CDS             complement(19475..21001)
                     /locus_tag="IVZ90_000438"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8261513.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21016..21663)
                     /locus_tag="IVZ90_000439"
     CDS             complement(21016..21663)
                     /locus_tag="IVZ90_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8261514.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21847..22176)
                     /locus_tag="IVZ90_000440"
     CDS             complement(21847..22176)
                     /locus_tag="IVZ90_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8261515.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22353..23081
                     /gene="sfsA"
                     /locus_tag="IVZ90_000441"
     CDS             22353..23081
                     /gene="sfsA"
                     /locus_tag="IVZ90_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8261516.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23097..24110
                     /locus_tag="IVZ90_000442"
     CDS             23097..24110
                     /locus_tag="IVZ90_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8261517.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24110..25387
                     /locus_tag="IVZ90_000443"
     CDS             24110..25387
                     /locus_tag="IVZ90_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8261518.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25450..28152
                     /locus_tag="IVZ90_000444"
     CDS             25450..28152
                     /locus_tag="IVZ90_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261519.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28304..28621
                     /locus_tag="IVZ90_000445"
     CDS             28304..28621
                     /locus_tag="IVZ90_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8261520.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28651..28971
                     /locus_tag="IVZ90_000446"
     CDS             28651..28971
                     /locus_tag="IVZ90_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8261521.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29079..30539
                     /locus_tag="IVZ90_000447"
     CDS             29079..30539
                     /locus_tag="IVZ90_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8261522.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30607..30828
                     /locus_tag="IVZ90_000448"
     CDS             30607..30828
                     /locus_tag="IVZ90_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261523.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30859..31041
                     /locus_tag="IVZ90_000449"
     CDS             30859..31041
                     /locus_tag="IVZ90_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8261524.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31041..31454
                     /locus_tag="IVZ90_000450"
     CDS             31041..31454
                     /locus_tag="IVZ90_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8261525.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31577..32758
                     /locus_tag="IVZ90_000451"
     CDS             31577..32758
                     /locus_tag="IVZ90_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261526.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            33147..33220
                     /locus_tag="IVZ90_000452"
     tRNA            33147..33220
                     /locus_tag="IVZ90_000452"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33181..33183,aa:Arg,seq:cct)
     gene            complement(33257..33457)
                     /locus_tag="IVZ90_000453"
     CDS             complement(33257..33457)
                     /locus_tag="IVZ90_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8261527.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(33762..34994)
                     /locus_tag="IVZ90_000454"
     CDS             complement(33762..34994)
                     /locus_tag="IVZ90_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8261528.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            35248..35817
                     /locus_tag="IVZ90_000455"
     CDS             35248..35817
                     /locus_tag="IVZ90_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8261529.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(35995..36435)
                     /locus_tag="IVZ90_000456"
     CDS             complement(35995..36435)
                     /locus_tag="IVZ90_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8261530.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            36593..37357
                     /gene="map"
                     /locus_tag="IVZ90_000457"
     CDS             36593..37357
                     /gene="map"
                     /locus_tag="IVZ90_000457"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8261531.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            37389..38312
                     /locus_tag="IVZ90_000458"
     CDS             37389..38312
                     /locus_tag="IVZ90_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8261532.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            38388..39527
                     /locus_tag="IVZ90_000459"
     CDS             38388..39527
                     /locus_tag="IVZ90_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8261533.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            39520..40320
                     /locus_tag="IVZ90_000460"
     CDS             39520..40320
                     /locus_tag="IVZ90_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8261534.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            40320..41147
                     /locus_tag="IVZ90_000461"
     CDS             40320..41147
                     /locus_tag="IVZ90_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8261535.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            41125..41859
                     /locus_tag="IVZ90_000462"
     CDS             41125..41859
                     /locus_tag="IVZ90_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8261536.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            41959..42825
                     /gene="rfbA"
                     /locus_tag="IVZ90_000463"
     CDS             41959..42825
                     /gene="rfbA"
                     /locus_tag="IVZ90_000463"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8261537.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            42839..43411
                     /gene="rfbC"
                     /locus_tag="IVZ90_000464"
     CDS             42839..43411
                     /gene="rfbC"
                     /locus_tag="IVZ90_000464"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8261538.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            43433..44461
                     /gene="rfbB"
                     /locus_tag="IVZ90_000465"
     CDS             43433..44461
                     /gene="rfbB"
                     /locus_tag="IVZ90_000465"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8261539.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            44559..45410
                     /gene="rfbD"
                     /locus_tag="IVZ90_000466"
     CDS             44559..45410
                     /gene="rfbD"
                     /locus_tag="IVZ90_000466"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8261540.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            45445..47478
                     /locus_tag="IVZ90_000467"
     CDS             45445..47478
                     /locus_tag="IVZ90_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261541.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            47522..48802
                     /locus_tag="IVZ90_000468"
     CDS             47522..48802
                     /locus_tag="IVZ90_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8261542.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            49012..49818
                     /locus_tag="IVZ90_000469"
     CDS             49012..49818
                     /locus_tag="IVZ90_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261543.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            49830..51050
                     /locus_tag="IVZ90_000470"
     CDS             49830..51050
                     /locus_tag="IVZ90_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261544.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            51040..52626
                     /locus_tag="IVZ90_000471"
     CDS             51040..52626
                     /locus_tag="IVZ90_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8261545.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            52665..54803
                     /locus_tag="IVZ90_000472"
     CDS             52665..54803
                     /locus_tag="IVZ90_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8261546.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(55171..56154)
                     /locus_tag="IVZ90_000473"
     CDS             complement(55171..56154)
                     /locus_tag="IVZ90_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8261547.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            56532..57923
                     /locus_tag="IVZ90_000474"
     CDS             56532..57923
                     /locus_tag="IVZ90_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8261548.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            57939..58979
                     /locus_tag="IVZ90_000475"
     CDS             57939..58979
                     /locus_tag="IVZ90_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8261549.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            58998..>60039
                     /locus_tag="IVZ90_000476"
     CDS             58998..>60039
                     /locus_tag="IVZ90_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8261550.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVS"
BASE COUNT        19666 a        10159 c        12498 g        17716 t
ORIGIN      
        1 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
       61 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
      121 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
      181 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
      241 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
      301 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
      361 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
      421 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
      481 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
      541 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
      601 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
      661 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
      721 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
      781 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
      841 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
      901 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
      961 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
     1021 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
     1081 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
     1141 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
     1201 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
     1261 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
     1321 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
     1381 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
     1441 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
     1501 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
     1561 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
     1621 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
     1681 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
     1741 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
     1801 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
     1861 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
     1921 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
     1981 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
     2041 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
     2101 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
     2161 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
     2221 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
     2281 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
     2341 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
     2401 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
     2461 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
     2521 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
     2581 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
     2641 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
     2701 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
     2761 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
     2821 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
     2881 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
     2941 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
     3001 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
     3061 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
     3121 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
     3181 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
     3241 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
     3301 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
     3361 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
     3421 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
     3481 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
     3541 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
     3601 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
     3661 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
     3721 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
     3781 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
     3841 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
     3901 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
     3961 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
     4021 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
     4081 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
     4141 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
     4201 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
     4261 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
     4321 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
     4381 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
     4441 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
     4501 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
     4561 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
     4621 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
     4681 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
     4741 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
     4801 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
     4861 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
     4921 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
     4981 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
     5041 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
     5101 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
     5161 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
     5221 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
     5281 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
     5341 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
     5401 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
     5461 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
     5521 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
     5581 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
     5641 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
     5701 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
     5761 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
     5821 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
     5881 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
     5941 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
     6001 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
     6061 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
     6121 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
     6181 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
     6241 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
     6301 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
     6361 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
     6421 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
     6481 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
     6541 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
     6601 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
     6661 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
     6721 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
     6781 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
     6841 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
     6901 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
     6961 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
     7021 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
     7081 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
     7141 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
     7201 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
     7261 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
     7321 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
     7381 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
     7441 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
     7501 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
     7561 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
     7621 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
     7681 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
     7741 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
     7801 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
     7861 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
     7921 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
     7981 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
     8041 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
     8101 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
     8161 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
     8221 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
     8281 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
     8341 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
     8401 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
     8461 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
     8521 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
     8581 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
     8641 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
     8701 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
     8761 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
     8821 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
     8881 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
     8941 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
     9001 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
     9061 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
     9121 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
     9181 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
     9241 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
     9301 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
     9361 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
     9421 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
     9481 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
     9541 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
     9601 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
     9661 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
     9721 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
     9781 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
     9841 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
     9901 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
     9961 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    10021 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    10081 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    10141 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    10201 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    10261 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    10321 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    10381 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    10441 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    10501 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    10561 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    10621 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    10681 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    10741 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    10801 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    10861 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    10921 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    10981 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    11041 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    11101 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    11161 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    11221 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    11281 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    11341 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    11401 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    11461 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    11521 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    11581 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    11641 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    11701 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    11761 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    11821 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    11881 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    11941 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    12001 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    12061 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    12121 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    12181 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    12241 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    12301 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    12361 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    12421 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    12481 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    12541 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    12601 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    12661 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    12721 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    12781 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    12841 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    12901 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    12961 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    13021 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    13081 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    13141 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    13201 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    13261 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    13321 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    13381 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    13441 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    13501 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    13561 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    13621 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    13681 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    13741 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    13801 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    13861 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    13921 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    13981 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    14041 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    14101 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    14161 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    14221 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    14281 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    14341 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    14401 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    14461 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    14521 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    14581 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    14641 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    14701 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    14761 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    14821 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    14881 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    14941 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    15001 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    15061 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    15121 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    15181 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    15241 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    15301 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    15361 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    15421 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    15481 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    15541 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    15601 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    15661 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    15721 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    15781 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    15841 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    15901 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    15961 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    16021 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    16081 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    16141 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    16201 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    16261 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    16321 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    16381 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    16441 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    16501 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    16561 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    16621 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    16681 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    16741 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    16801 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    16861 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    16921 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    16981 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    17041 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    17101 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    17161 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    17221 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    17281 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    17341 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    17401 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    17461 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    17521 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    17581 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    17641 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    17701 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    17761 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    17821 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    17881 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    17941 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    18001 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    18061 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    18121 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    18181 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    18241 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    18301 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    18361 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    18421 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    18481 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    18541 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    18601 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    18661 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    18721 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    18781 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    18841 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    18901 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    18961 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    19021 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    19081 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    19141 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    19201 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    19261 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    19321 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    19381 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    19441 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    19501 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    19561 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    19621 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    19681 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    19741 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    19801 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    19861 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    19921 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    19981 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    20041 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    20101 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    20161 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    20221 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    20281 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    20341 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    20401 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    20461 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    20521 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    20581 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    20641 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    20701 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    20761 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    20821 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    20881 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    20941 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    21001 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    21061 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    21121 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    21181 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    21241 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    21301 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    21361 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    21421 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    21481 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    21541 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    21601 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    21661 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    21721 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    21781 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    21841 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    21901 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    21961 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    22021 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    22081 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    22141 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    22201 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    22261 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    22321 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    22381 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    22441 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    22501 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    22561 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    22621 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    22681 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    22741 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    22801 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    22861 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    22921 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    22981 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    23041 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    23101 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    23161 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    23221 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    23281 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    23341 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    23401 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    23461 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    23521 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    23581 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    23641 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    23701 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    23761 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    23821 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    23881 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    23941 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    24001 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    24061 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    24121 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    24181 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    24241 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    24301 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    24361 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    24421 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    24481 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    24541 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    24601 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    24661 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    24721 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    24781 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    24841 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    24901 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    24961 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    25021 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    25081 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    25141 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    25201 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    25261 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    25321 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    25381 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    25441 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    25501 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    25561 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    25621 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    25681 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    25741 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    25801 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    25861 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    25921 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    25981 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    26041 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    26101 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    26161 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    26221 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    26281 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    26341 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    26401 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    26461 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    26521 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    26581 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    26641 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    26701 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    26761 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    26821 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    26881 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    26941 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    27001 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    27061 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    27121 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    27181 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    27241 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    27301 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    27361 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    27421 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    27481 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    27541 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    27601 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    27661 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    27721 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    27781 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    27841 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    27901 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    27961 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    28021 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    28081 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    28141 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    28201 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    28261 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    28321 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    28381 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    28441 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    28501 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    28561 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    28621 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    28681 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    28741 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    28801 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    28861 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    28921 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    28981 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    29041 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    29101 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    29161 tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga
    29221 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    29281 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    29341 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    29401 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    29461 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    29521 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    29581 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    29641 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    29701 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    29761 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    29821 ggcaactgat cattgatttg gtgcaaaaat atattgcaga gccattccta ggattatctc
    29881 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    29941 ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    30001 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    30061 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    30121 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    30181 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    30241 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    30301 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    30361 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    30421 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    30481 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    30541 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    30601 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    30661 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    30721 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    30781 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    30841 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    30901 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    30961 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    31021 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    31081 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    31141 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    31201 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    31261 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    31321 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    31381 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    31441 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    31501 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    31561 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    31621 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    31681 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    31741 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    31801 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    31861 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    31921 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    31981 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    32041 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    32101 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    32161 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    32221 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
    32281 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
    32341 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
    32401 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
    32461 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
    32521 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
    32581 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
    32641 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
    32701 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
    32761 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
    32821 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
    32881 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
    32941 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
    33001 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
    33061 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
    33121 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
    33181 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tacaaataac ccctgtatcc
    33241 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
    33301 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
    33361 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
    33421 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
    33481 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
    33541 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
    33601 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
    33661 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
    33721 ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt
    33781 cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat
    33841 cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca
    33901 tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga
    33961 ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt
    34021 gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc
    34081 agttttgcta agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta
    34141 aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa
    34201 atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt
    34261 gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta
    34321 ggcaatccga taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct
    34381 cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca
    34441 gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt
    34501 ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg
    34561 acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca
    34621 ttcgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg
    34681 gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt
    34741 ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg
    34801 atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt
    34861 gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct
    34921 ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta
    34981 aaatcagata acataataaa aattcctcct caccattttt tctggtaact attagcatga
    35041 taccatttaa ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt
    35101 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
    35161 aactaatttt gcatatcaaa aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt
    35221 ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac tagagaacag
    35281 attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt tactgcgaga
    35341 aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt taaaaatatg
    35401 gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata tgaagtgttc
    35461 actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat ccattttgca
    35521 agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg aggaaaagag
    35581 atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc agcttatgtg
    35641 aatctttcag aagagcagat cgaatctttg tataatggga catggatcat agctactggg
    35701 atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat tgaaaagatg
    35761 atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat cgattgaata
    35821 tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc
    35881 gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga atgcaatgaa
    35941 taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag tatattaaat
    36001 ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt cttcagcatt
    36061 cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa aagcagcatc
    36121 gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc cgcagacacc
    36181 gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta cgatctcatc
    36241 aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg cctcctcgaa
    36301 ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca gattttcaaa
    36361 tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag catatacgat
    36421 ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta tgaattatac
    36481 caaaacatca ttgaaaagaa aataaaacct aaagacacct ggggaatgtc atttggctaa
    36541 atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa atatgattac
    36601 tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat tagcagatgt
    36661 acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata ttgaggtatt
    36721 tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct ttgaagggta
    36781 tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc ctcgtaaaaa
    36841 gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga aaggcggcat
    36901 ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg accgattgat
    36961 ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag ggaatcggat
    37021 tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag cgatcgttcg
    37081 tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag ttcctcatta
    37141 cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga tcgaacctat
    37201 ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg cgtatacaag
    37261 agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag atggtccaag
    37321 aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa taggtacggg
    37381 agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg cgctttattg
    37441 agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc gtcgttgctt
    37501 attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt ttaccgtttc
    37561 tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct gagaccgttt
    37621 accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga ggcttgttat
    37681 ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct ttgcaaattg
    37741 cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct
    37801 cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc gttcttgggt
    37861 tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg aactttatcg
    37921 atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc atcatgttct
    37981 tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt ccgggcacga
    38041 tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat gctaggtatt
    38101 ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc atgctttggc
    38161 ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt gttcaagaat
    38221 acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat
    38281 tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg taaatttata
    38341 tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg cttgtttctt
    38401 ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta acacctgtat
    38461 tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga agaataaata
    38521 aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt atatcgactt
    38581 tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg attgcttctt
    38641 tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct aaacaaaaaa
    38701 gtatagggat cttgattgct tctcttatta tttattttgt agcagatata cgtattgatt
    38761 ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt cccgtaacga
    38821 ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg gatgggctgg
    38881 cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat tttttcctcc
    38941 atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt attatcggtt
    39001 tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga gctctattcc
    39061 tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact tttgtttcat
    39121 cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt gctatcatta
    39181 ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt catcaccgac
    39241 taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg atggctttga
    39301 tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg atcgtattga
    39361 ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg atcaatgaga
    39421 aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac cgtacgcaag
    39481 taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa attaaaccag
    39541 ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac actagataat
    39601 tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga caacaattca
    39661 cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat cgatatctat
    39721 tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat agctagcgat
    39781 tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac agttacagca
    39841 ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa gattcttaat
    39901 gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga ttacgttttg
    39961 cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa
    40021 tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg tagttttatg
    40081 gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt cttcatgtac
    40141 tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt attgtacaca
    40201 cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc aaaactgttt
    40261 cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg ttttttctga
    40321 tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt caatctccag
    40381 cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt atttacgata
    40441 atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg
    40501 atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa gcagatgaag
    40561 gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat tattttgaac
    40621 tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg attttttctg
    40681 ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca gctcaagcag
    40741 tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt gttgcctggt
    40801 ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta gattttttgg
    40861 atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc cttcctgtac
    40921 gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat cgataccgtt
    40981 ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat ttatccagtc
    41041 accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa aaccggcata
    41101 tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg
    41161 gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga tagtatttta
    41221 tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt agatgataca
    41281 tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt ttcggggcct
    41341 cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg agatctaatc
    41401 tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat cactgactat
    41461 tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt ggacagcgag
    41521 ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg atggcttaat
    41581 aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc tttaaaaccc
    41641 gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg tttgctggga
    41701 ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca tgatttaaac
    41761 gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg
    41821 attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt taaatcctta
    41881 atatttccat gacaaatata acaattgatg agataataac aaagtgaaat ttgaaatcaa
    41941 aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg aacacgtctg
    42001 taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa accaatgatt
    42061 tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat tatttcaacg
    42121 ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt aggactgcag
    42181 atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat cattggagaa
    42241 gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta ctatggtggc
    42301 ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac agtatttggc
    42361 tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa tatgcaagct
    42421 ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac tggcctttat
    42481 ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga acgtggagaa
    42541 ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc cgtagaactt
    42601 atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct tgaagcatct
    42661 acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt
    42721 gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca gccattgaaa
    42781 aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt ccttacagat
    42841 gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg tctttggcga
    42901 tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac atggattgaa
    42961 atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc ttcgggggct
    43021 tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca ctggagcagt
    43081 tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat gggacggatt
    43141 tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg cccatggatt
    43201 cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact ataatgcaga
    43261 agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc caattgatcc
    43321 atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag aatttgaagc
    43381 agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc ttatgaaaaa
    43441 catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt acgtggtaaa
    43501 caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag gaaacaaaga
    43561 gaacttggcc ggacttccta gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc
    43621 agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg cggcagaatc
    43681 ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata tcattggtac
    43741 gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg tatctactga
    43801 cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg aaggcgaagg
    43861 agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt ctacaaaagc
    43921 tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg caaccatttc
    43981 aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc ctcgccaagt
    44041 aacgagtatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga atgttcgtga
    44101 ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga aaggcagaat
    44161 tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg tgatggaatt
    44221 gatcttagaa atgatgggcc aaccaaaaga cgcatacgac catgtaaacg accgcgcagg
    44281 gcacgatttg cgttatgcaa tcgattcgac aaaattgcga gaagagctag gttggcagcc
    44341 tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg aagaacatga
    44401 agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa atggacaata
    44461 atacagagga aatttcactg tattaatgga aaaacacatg tgaggcaggc gctcgcctgt
    44521 ctctttttta aaataaaaat caaggagcgg ataaccatat gtttttatta actggaggaa
    44581 atggacaatt aggtacagaa ttgcgccatt tattagatga aaaaaatctg ccatatgttt
    44641 caacagatgc aaaagacttg gatatcacag atgctgagaa aacaatggct tatattacag
    44701 aattaaaacc cgatgttatt ttccattgcg cagcttacac agcagtagat aaagcagaag
    44761 aagaagcaaa agaattagat gagaaaatca atgtcgatgg cacaagaaat gtagcacttg
    44821 cagcaaaagc tgtgaatgca caattcgtct atatcagtac agactatgtt ttcgatggaa
    44881 aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat gaatatggtc
    44941 gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat tatatcatac
    45001 gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg caacgcctag
    45061 cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca acttggacac
    45121 gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt ggtatttatc
    45181 atttgtcgaa cgaaaatagc tgttcttggt atgagttcgc aaaagaaata ttgaaagata
    45241 cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca acacgtccgc
    45301 agtattcagt catgagcctg aaaaagacag aagcgttagg ttttgtcata ccaacttggc
    45361 aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa tttccttttt
    45421 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
    45481 aagccaaaag cgaatcaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
    45541 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
    45601 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
    45661 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
    45721 tcacaagctg aagaggactt ttcgttggtg aacgaggatt attcattaaa cggacaaaac
    45781 atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac
    45841 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
    45901 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag gaaaaaagct
    45961 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
    46021 caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt ttatcattat
    46081 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
    46141 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
    46201 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
    46261 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
    46321 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
    46381 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
    46441 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
    46501 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
    46561 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
    46621 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
    46681 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
    46741 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
    46801 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
    46861 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
    46921 tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
    46981 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
    47041 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
    47101 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
    47161 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
    47221 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
    47281 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
    47341 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
    47401 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
    47461 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag
    47521 agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
    47581 gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt ttccagcaac
    47641 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
    47701 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
    47761 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
    47821 gtttcctttg gttattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
    47881 tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt
    47941 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
    48001 tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag
    48061 tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga tagcagtcgt
    48121 tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
    48181 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
    48241 tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
    48301 aaaagtcaat ttgtttaccg atcgagaaga tatttggcat aaattctatc agacgctagg
    48361 agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc gaggaaacca
    48421 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
    48481 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
    48541 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
    48601 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
    48661 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
    48721 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
    48781 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
    48841 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
    48901 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
    48961 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
    49021 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg
    49081 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
    49141 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
    49201 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
    49261 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
    49321 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
    49381 acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
    49441 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
    49501 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
    49561 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
    49621 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
    49681 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
    49741 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
    49801 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
    49861 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
    49921 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
    49981 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
    50041 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
    50101 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
    50161 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
    50221 tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact tattcaagcg
    50281 ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat attttgattg
    50341 tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg gaaaaattta
    50401 cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc agttcgattc
    50461 gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa tatggcgata
    50521 cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg
    50581 atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag
    50641 cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga gaaaaactat
    50701 acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt ttaggaattg
    50761 cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta gatggtatca
    50821 agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag ttaggtttgt
    50881 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
    50941 tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
    51001 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
    51061 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
    51121 gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg agcaaaacaa
    51181 tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag cgaatttgct
    51241 caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta tgctggacag
    51301 acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca
    51361 ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac
    51421 ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg ggcttcttat
    51481 ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt
    51541 gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt aggtgataca
    51601 gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt caaaatgcga
    51661 ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt agcacaaagt
    51721 tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg
    51781 aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca
    51841 ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct
    51901 agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat cgattcgaat
    51961 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt accagagttc
    52021 gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg
    52081 gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc tgaagtcaca
    52141 aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct tcttgaggaa
    52201 aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa
    52261 agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac ttcgaaaaaa
    52321 atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa
    52381 atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt gtcctataag
    52441 ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga agaagtcaat
    52501 caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat cctttgcgag
    52561 caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg gatcattgaa
    52621 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
    52681 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
    52741 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
    52801 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
    52861 ttaagaccca aagcggtttc aaggtacgtt tatcagggaa aatgagagga aaagccatct
    52921 tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac ggtaaatacc
    52981 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
    53041 taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga
    53101 agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca gatttgaaag
    53161 agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata cttatgcctg
    53221 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg gatcaaacgt
    53281 atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
    53341 gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga aaagaaaatg
    53401 gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa tttatagcac
    53461 tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa
    53521 acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat gcagatggca
    53581 atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg ggaaataatt
    53641 atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc ggtttccgta
    53701 aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg
    53761 cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa
    53821 ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa gcattgacag
    53881 atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt
    53941 atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga
    54001 atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa
    54061 attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa gaattatttg
    54121 cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac attccattca
    54181 attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac tttttattct
    54241 tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt tcttatgcac
    54301 agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc
    54361 atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc
    54421 gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg
    54481 cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat
    54541 tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca
    54601 atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag
    54661 atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc
    54721 cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagac ggaatcggcg
    54781 acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc
    54841 acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa
    54901 taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat
    54961 tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc
    55021 gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat
    55081 tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt
    55141 caaagaatcg atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc
    55201 ctctacaaaa gccactctat caattatcat gggtttaaaa taagaagtga tggagataac
    55261 cagattgttc ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat
    55321 ttcgttctca gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc
    55381 taaagcactg gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt
    55441 tcctttatac atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga
    55501 taaacacaaa ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt
    55561 tttaggagac ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt
    55621 gacatggttt ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt
    55681 tagcttactt gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt
    55741 gaattcgccg gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc
    55801 ttcacttgaa cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt
    55861 tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc
    55921 tggaaaataa tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat
    55981 gccaatcagc aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt
    56041 tttataacca ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg
    56101 acatatattt ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata
    56161 ttacctcact ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt
    56221 cttcacgtaa tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg
    56281 aaaaatataa aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt
    56341 taatttacca acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt
    56401 cttgattaga ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct
    56461 tataaactag gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta
    56521 ggagtgtata tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat
    56581 taatggatgt tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg
    56641 gagagatccc attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga
    56701 tttttattgc gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg
    56761 acatcgtgtt tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga
    56821 cttttgcggg ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg
    56881 gtacgctttt cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc
    56941 gagataaaaa ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt
    57001 ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg
    57061 caaacatcaa aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag
    57121 aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga
    57181 atacaacatt tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa
    57241 gtattattga agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt
    57301 ttgatatcat cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag
    57361 caattttagt caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa
    57421 aaaatcaaaa ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa
    57481 aatctggacc agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta
    57541 ttcgagctgt ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt
    57601 cgatcgtagg accacgtcct gaacgttctt tcttcgtcga tcagttcaat gcagaaaacc
    57661 cttattacta cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg
    57721 gaaaatatgt ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat
    57781 attcattgat gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa
    57841 tgtcctctca aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc
    57901 cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca
    57961 taatcgtacc agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag
    58021 aattggatta tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac
    58081 aggaaatcat tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca
    58141 atcaagggcc cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct
    58201 tattcttcga tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc
    58261 tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg
    58321 agggaagaat cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg
    58381 aagagtttat aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga
    58441 acaaatgcta tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata
    58501 gttgtgagga tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga
    58561 acccaaaagt agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc
    58621 gacctaacaa gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg
    58681 atattaataa agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt
    58741 ctatttatag tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat
    58801 tgagtgatcc agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa
    58861 aaataacaaa gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg
    58921 gaagcttcgt tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt
    58981 taatggagga aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg
    59041 gagcagagtc tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg
    59101 attttttgat ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat
    59161 taggaggccg tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag
    59221 aagtgaataa gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg
    59281 gcgggggaat taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata
    59341 gccatgcaac tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt
    59401 taaccaaatt gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg
    59461 aaagtctgtt tcaaaaccat ccctttgaga tagttcataa tggtattgat ttagagcttt
    59521 atagtaaaaa ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta
    59581 cttttgttgt gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag
    59641 agattttttt agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag
    59701 ggtcacttag aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa
    59761 aatttttagg agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa
    59821 tgccttctct ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa
    59881 aattagtctt agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg
    59941 tatcattaga tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta
    60001 ataaaataac acaagaacta aagagttatg atgtttcgt
//
LOCUS       DACDBG010000008        59483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDBG010000008 DACDBG010000000
VERSION     DACDBG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.008"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..502
                     /locus_tag="IVZ90_000477"
     CDS             68..502
                     /locus_tag="IVZ90_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261551.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(570..1892)
                     /locus_tag="IVZ90_000478"
     CDS             complement(570..1892)
                     /locus_tag="IVZ90_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8261552.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(2046..3686)
                     /locus_tag="IVZ90_000479"
     CDS             complement(2046..3686)
                     /locus_tag="IVZ90_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8261553.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4016..4486
                     /locus_tag="IVZ90_000480"
     CDS             4016..4486
                     /locus_tag="IVZ90_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8261554.1"
                     /translation="MQEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQVIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILTGLFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            4870..5973
                     /locus_tag="IVZ90_000481"
     CDS             4870..5973
                     /locus_tag="IVZ90_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8261555.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            6257..6937
                     /locus_tag="IVZ90_000482"
     CDS             6257..6937
                     /locus_tag="IVZ90_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8261556.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVGPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            7027..8061
                     /locus_tag="IVZ90_000483"
     CDS             7027..8061
                     /locus_tag="IVZ90_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8261557.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            8075..8410
                     /locus_tag="IVZ90_000484"
     CDS             8075..8410
                     /locus_tag="IVZ90_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261558.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            8394..10874
                     /locus_tag="IVZ90_000485"
     CDS             8394..10874
                     /locus_tag="IVZ90_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261559.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            10930..12489
                     /locus_tag="IVZ90_000486"
     CDS             10930..12489
                     /locus_tag="IVZ90_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8261560.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYRDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASIFRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTFKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            12486..13850
                     /locus_tag="IVZ90_000487"
     CDS             12486..13850
                     /locus_tag="IVZ90_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8261561.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTPRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            14023..15093
                     /locus_tag="IVZ90_000488"
     CDS             14023..15093
                     /locus_tag="IVZ90_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261562.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            15108..15776
                     /locus_tag="IVZ90_000489"
     CDS             15108..15776
                     /locus_tag="IVZ90_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261563.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            15796..16374
                     /locus_tag="IVZ90_000490"
     CDS             15796..16374
                     /locus_tag="IVZ90_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8261564.1"
                     /translation="MIHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLIDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            16776..17687
                     /locus_tag="IVZ90_000491"
     CDS             16776..17687
                     /locus_tag="IVZ90_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8261565.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKA
                     KESSDDTEQETLVKIGKNDRTLYTDDSLTTPLATINGGELTEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKVLEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(17775..18692)
                     /locus_tag="IVZ90_000492"
     CDS             complement(17775..18692)
                     /locus_tag="IVZ90_000492"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8261566.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            18894..19331
                     /gene="zapA"
                     /locus_tag="IVZ90_000493"
     CDS             18894..19331
                     /gene="zapA"
                     /locus_tag="IVZ90_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8261567.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            19390..19938
                     /locus_tag="IVZ90_000494"
     CDS             19390..19938
                     /locus_tag="IVZ90_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8261568.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            20053..22413
                     /locus_tag="IVZ90_000495"
     CDS             20053..22413
                     /locus_tag="IVZ90_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8261569.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTFT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQKKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            22500..22814
                     /gene="trxA"
                     /locus_tag="IVZ90_000496"
     CDS             22500..22814
                     /gene="trxA"
                     /locus_tag="IVZ90_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8261570.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            22996..24774
                     /gene="uvrC"
                     /locus_tag="IVZ90_000497"
     CDS             22996..24774
                     /gene="uvrC"
                     /locus_tag="IVZ90_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8261571.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            24919..25818
                     /locus_tag="IVZ90_000498"
     CDS             24919..25818
                     /locus_tag="IVZ90_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261572.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            25811..27073
                     /locus_tag="IVZ90_000499"
     CDS             25811..27073
                     /locus_tag="IVZ90_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261573.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            27234..29132
                     /locus_tag="IVZ90_000500"
     CDS             27234..29132
                     /locus_tag="IVZ90_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8261574.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(29431..30150)
                     /locus_tag="IVZ90_000501"
     CDS             complement(29431..30150)
                     /locus_tag="IVZ90_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8261575.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            30487..30819
                     /locus_tag="IVZ90_000502"
     CDS             30487..30819
                     /locus_tag="IVZ90_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261576.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            30806..32809
                     /locus_tag="IVZ90_000503"
     CDS             30806..32809
                     /locus_tag="IVZ90_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8261577.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            32828..33298
                     /locus_tag="IVZ90_000504"
     CDS             32828..33298
                     /locus_tag="IVZ90_000504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8261578.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            33319..33903
                     /locus_tag="IVZ90_000505"
     CDS             33319..33903
                     /locus_tag="IVZ90_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8261579.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            33918..34904
                     /locus_tag="IVZ90_000506"
     CDS             33918..34904
                     /locus_tag="IVZ90_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8261580.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            34894..35205
                     /locus_tag="IVZ90_000507"
     CDS             34894..35205
                     /locus_tag="IVZ90_000507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8261581.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            35227..37008
                     /locus_tag="IVZ90_000508"
     CDS             35227..37008
                     /locus_tag="IVZ90_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8261582.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            37001..38377
                     /locus_tag="IVZ90_000509"
     CDS             37001..38377
                     /locus_tag="IVZ90_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8261583.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            38384..39019
                     /locus_tag="IVZ90_000510"
     CDS             38384..39019
                     /locus_tag="IVZ90_000510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8261584.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            39049..40404
                     /locus_tag="IVZ90_000511"
     CDS             39049..40404
                     /locus_tag="IVZ90_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8261585.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(40596..41990)
                     /locus_tag="IVZ90_000512"
     CDS             complement(40596..41990)
                     /locus_tag="IVZ90_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8261586.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            42082..43053
                     /locus_tag="IVZ90_000513"
     CDS             42082..43053
                     /locus_tag="IVZ90_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8261587.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            43050..43831
                     /locus_tag="IVZ90_000514"
                     /pseudo
     CDS             43050..43831
                     /locus_tag="IVZ90_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(44000..44371)
                     /locus_tag="IVZ90_000515"
     CDS             complement(44000..44371)
                     /locus_tag="IVZ90_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8261588.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            44505..44948
                     /locus_tag="IVZ90_000516"
     CDS             44505..44948
                     /locus_tag="IVZ90_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8261589.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(44991..45800)
                     /gene="yidA"
                     /locus_tag="IVZ90_000517"
     CDS             complement(44991..45800)
                     /gene="yidA"
                     /locus_tag="IVZ90_000517"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8261590.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(45819..47186)
                     /locus_tag="IVZ90_000518"
     CDS             complement(45819..47186)
                     /locus_tag="IVZ90_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8261591.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(47170..48000)
                     /locus_tag="IVZ90_000519"
     CDS             complement(47170..48000)
                     /locus_tag="IVZ90_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8261592.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            48163..48600
                     /locus_tag="IVZ90_000520"
     CDS             48163..48600
                     /locus_tag="IVZ90_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8261593.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            48676..49296
                     /locus_tag="IVZ90_000521"
     CDS             48676..49296
                     /locus_tag="IVZ90_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8261594.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            49485..49991
                     /locus_tag="IVZ90_000522"
     CDS             49485..49991
                     /locus_tag="IVZ90_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8261595.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            50008..50907
                     /locus_tag="IVZ90_000523"
     CDS             50008..50907
                     /locus_tag="IVZ90_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8261596.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            50994..51879
                     /locus_tag="IVZ90_000524"
                     /pseudo
     CDS             50994..51879
                     /locus_tag="IVZ90_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(52071..52142)
                     /locus_tag="IVZ90_000525"
     tRNA            complement(52071..52142)
                     /locus_tag="IVZ90_000525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52107..52109),aa:Arg,seq:ccg)
     gene            52202..53536
                     /gene="rpoN"
                     /locus_tag="IVZ90_000526"
     CDS             52202..53536
                     /gene="rpoN"
                     /locus_tag="IVZ90_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8261597.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            53739..54776
                     /locus_tag="IVZ90_000527"
     CDS             53739..54776
                     /locus_tag="IVZ90_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8261598.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            54817..55818
                     /gene="gap"
                     /locus_tag="IVZ90_000528"
     CDS             54817..55818
                     /gene="gap"
                     /locus_tag="IVZ90_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8261599.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            55928..57118
                     /locus_tag="IVZ90_000529"
     CDS             55928..57118
                     /locus_tag="IVZ90_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8261600.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            57222..57977
                     /locus_tag="IVZ90_000530"
     CDS             57222..57977
                     /locus_tag="IVZ90_000530"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8261601.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            58093..59391
                     /gene="eno"
                     /locus_tag="IVZ90_000531"
     CDS             58093..59391
                     /gene="eno"
                     /locus_tag="IVZ90_000531"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8261602.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        19743 a        10408 c        12505 g        16827 t
ORIGIN      
        1 tgaaaaaaag agtatactat gataaaaaaa ttcattttaa aaaatcagaa attggctggt
       61 gatttggatg ttacgggaaa tgcgtttaga agatgcagaa gcactacgtg aaatcaatgc
      121 ggatcaatta gggtatgata taccattaac cgttacggtg agacaaatga agaaactgct
      181 gaaggagcca gagaaaaatt ttttcttggt ttatgaagat cctacttcta aaaaagcagc
      241 tggctatgta caagcggagt cctatgaatc gatttttagc gatccaatgt tcaatattat
      301 ggcacttgct gtgtctaaat atgctgaaaa aagaggcatc ggaaaggctt tgatgactgg
      361 cgtagaagaa gaagcgaaaa aacgaggaat aacgacaata aggcttaatt ctgcagaata
      421 cagagtagaa gcacaccaat tttatgaaca catcggttat cattccgata aaatgcaaaa
      481 aagatttttg aaaatcctgt aaatctttat caaaaaaata cccgatcacc aagccgatac
      541 ttgatgatca ggtatttttg tttttttgac taagaaagta attgcatcaa gaaaccagag
      601 atgatcaaaa cgatggcacc accaagtcga ttgcccattt gagcaaaagc aataagttcc
      661 atccgattag ctgcagataa gacggctaca tttcctgttc cgcccatcga gttgttgcat
      721 aagccagcag taatagttga ttcaattgga tataaaccaa acaaacgacc gacaaatcct
      781 gaaacaactg aaatcgcgat aacacttgtc agacataaga caacaaattg ccatgtcaaa
      841 gcagaagcta atacattcag attcagcaac gcaataccga taccggcaag aacagctgct
      901 gtcaagtttt tgacaatcaa gttattgaac atgatagctg catcttcata atactctggt
      961 aataggttgg cgattttaca gatgacaacg ataataatca taaatgcgta tgcatggact
     1021 ttaggaacaa aatagttacc aatttgtcct aagatgaaga aagtcaatga taccatcaaa
     1081 ccaacaccta actgtggaat ggaaggtttc acagttcgag gttttccatc gccaagatcg
     1141 cctttttcca ttaatttacc attgccgttg aattttggca ttgattgtcc agcacgagca
     1201 actaatgctg caccgatgat agctaagaca tttgtcattg ctgtagcagg aatcaggcgg
     1261 gaaatgatag aagaagcatc cgtacctaaa acatttgcat aaatcgttga taaaggaacg
     1321 gctccggctc caacaccacc agccatcgca ggtaatgaga tgtataaaac ggaatttgca
     1381 aaaccatttc cgataagcat gcccacgagt cctacggcaa aaaaggagac gaccattgaa
     1441 ataagtgcaa cgggaataaa acgaacagaa gctttcatca atagattacg gttcatacca
     1501 aggatgctac cagtaatcaa ggcagcaata taaaagtcca aaaagcccat attgctgaca
     1561 aagttttctg tggatttcac tacatattca ggtaagatcc caaatgtagc aatcgcagca
     1621 gaagcgaaga tcgcaaaaac ggaaccacca cccaaataac ttttgacgat agggattttt
     1681 gtcccgataa aatgaaataa attaccgaaa ataaccaaaa ctgcgattgg tccaatcata
     1741 ttcgtcggta atttccctaa agccattgca atgatcagga caaggaccat gatcaaataa
     1801 agtggcaaac ttacgccggt gatttttgtg gaccaaaaac cttctttttt cttttcctca
     1861 actgcagttg cagttgttgt tttttgtgcc atttttacag atcctttctt attctgtatc
     1921 atatgaaaaa agccaaagaa aagcgacaaa agagcaggag ttggctggcc gctgttttgc
     1981 cgcagtcgct tttttctttt tagacgtcat acaaagtttc tgtatgttct tttactataa
     2041 cgatcttaat cttctaaaga tttatattca ggaacccatt tagcagattc aactgcttct
     2101 ttgatatccg tgatttcttc acgattcaag ttttgtgcga caactgattt tgcgacagtt
     2161 tcagcaatcg tttgagaaaa ttcagtgatt tttgctactg gaggcaagat agctgctcct
     2221 ggttttgtta catcaacgat tccgcctaat gctcgactag cttgagaaat gatttcagca
     2281 tttactcgtg tagctgttga agcaatcaaa ccaagtccta aacctgggta catcaaagca
     2341 ttatttgctt gaccgatttg gtaagttaca ccattatatt caacatcagc agcagggata
     2401 cctgttccaa ctaatgcttt tccgtccgtc cattctatta aatcttttgc ttttgcttct
     2461 gccaattttg ttggattaga caaaggcatg atgatcggac gtggtgtatg agcagccatt
     2521 tctttcacga tttcttcagt gaatgctcca ggttgggtag aagtaccaat catgattgtc
     2581 ggatggatcg cttttacaac tgcttctaag tttgtcaatt catcagcatt agtgaaatct
     2641 gagcgtttgc gagcgaatgg gatttgacca ggagtcaatc cttctgtatc ttcaaacaat
     2701 aaaccttgtt tatctacttg atagaaatgt tggcgtgctt cttcttcagg tacaccttga
     2761 cggatcatct catctaaaag gatgtttgcg atacccacac cagcagtacc agcaccaaat
     2821 gtcaaaatta tttgatcttt caattgttca ccggatatat tcaatccgcc taatacacct
     2881 gctaggacaa caattcctgt accttggata tcgtcgttga aagtagtgat ttgatcttcg
     2941 tatttttcta agatcgctgc agcatttcct cgtccaaaat cttcccaatg caataacaat
     3001 tctgggaata attcggtagc tgattgaacg aattgatcga tgaattcata gtaagtttcg
     3061 ccttctacac gagcatgacg attgcctaag tataagggat tattcaataa ttcttcatta
     3121 ttagtaccag cgtcgatcga tacaggcaaa acttgtgctg gattaatacc agcagcagca
     3181 gtatagacca tcaatttgcc gatagcgatg tcaacaccgt tcacacccca gtcgcccata
     3241 cctaagattc cttctgcatc agtcacaaca atcaaacgaa tgtctcgtcc atctgccgcg
     3301 ttcttcaaac ttgctttgat gttttctggt tcgtcgattg aaaggaatgc agcatcttgc
     3361 ggtttcaaga aaatttcgtt gtattgttcg attgaatccg cgacgacagg atcataaacg
     3421 acaggcatga attcaactaa atgttcgccc atcaatttat aaaacaacgt ccgattcgta
     3481 ttgaatagat tcattaagaa gatacgtttt tccaaatctg tttgtttgct taagtattga
     3541 ccataagctt gaacagattg ttgttccagt gtttggacag tacttggaag cataccagtg
     3601 attccatatt ttgcccgttc ttcttttgta aaagctgttc ctttatttag aaaaggatta
     3661 tttaataagt ttaaccctgt aagcatgaat gattcctcct aagttttaat acaaacgaag
     3721 tatagaagtc agatgatgat atagtcaaaa agagcaggta tgataaattt tatttatatg
     3781 taatatagcc aaaagaaatc aaaatagcag tatttgttct ttttatatcg acaatccttg
     3841 cttgaatact atgcgtatct cttatatgct agagaaagaa aagtagaaga ataagggata
     3901 gatggaatgg ataaatagga tttctgagga aaaaagcaca tgatagcgtt gataggagtc
     3961 acctatgtca ggtgataaaa atacataagt agaaaattaa gaggaggaac agaaaatgca
     4021 agaagttcaa ttgatcagaa aaagcgaact atcagaaggc ggttgcaatg catgtggcgt
     4081 agtagaagcg acaagctata cattaaaatt agattccaat caagtaataa tctctgaact
     4141 gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa ggttttactg gagaagatat
     4201 ttatgagatg ttttcagaag tccgtcagtt aaaaaaagga gaaaaatgca tagaggttca
     4261 tcatgagagc ccgaatgtga ggttcaaacg tggtgataat gagatgatct tcaagaatca
     4321 tgtgtctgat cacactgaac tatatgaaat cgtgaatcaa atactgactg ggctttttga
     4381 attaggacct tatgagttca aggaagaaaa cggtaatcca aaattaaatg aagaatggca
     4441 agaaacgatt gaaacacaaa gaaacaatcc acacttattt caatagtctt ccacagtatt
     4501 tcgtaaaaat gttgatgaaa aagaaacacc gtgaatcaga ggttgtgaaa aagtcgctta
     4561 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga
     4621 cttaataccg agaaaatggc ttttgaacac cgtttattca aaaaaaggga ctttgacacg
     4681 aattttgtca aagtcccatc ggttttttat ctatttttat tacaatcatt tcttccagag
     4741 tagtgacatc tggaatggcc gaaatgtaaa cagattattt gcctgtatca atgtcgaaga
     4801 gtaatataaa aaagtgtaaa cgctatcata ttgttcgtca ttcaattttt atagagagaa
     4861 ggcattgaga tggaaaaaac atatcaagca ggtacaaatg aaggaatagt agattttatc
     4921 aatatggaag atttagaaat cgcggcttca caagtgattc cagctggtgg ttatggttac
     4981 atcagcagtg gtgctggcga cttatttact tatcaagaaa atgaacgggc attcaatcat
     5041 cgtttgatta ttcctcatgt attaagagat gtagaattac ccgatacaac tacacatttt
     5101 gacgaagaaa tgctgactgc accaatcatc atggcaccag ttgctgctca tggattagct
     5161 catgtgaagg cggagaaagc ttctgcaaaa ggtgtagcag attttgggac gatctataca
     5221 gcaagttctt acgcttcttg tactttagaa gagataagag aagcaggcgg ggagaaagca
     5281 ccgcaatggt ttcaatttta tatgagcaaa gataatggaa tcaatttgga tattcttgaa
     5341 gtggccaagc gtaatggagc aaaagcaatc gtattgacag cggatgcaac agttggcgga
     5401 aaccgtgaaa cagaccgcag aaacgggttt accttcccgc tgccaatgcc gatcgttcag
     5461 gcttatcaat caggagtagg ccaaacgatg gatgctgtat ataaatcttc caaacaaaaa
     5521 cttagtccaa aagatgtaga atttatcgct gctcattctg atttgccagt ctacgtgaaa
     5581 ggtgttcaat cagaagaaga tgtctatcga tcattagaat ctggtgctgg tggaatttgg
     5641 gtatctaacc atggtggccg tcaattagat ggggggcctg ctgcgtttga ttcgttgcaa
     5701 tatgtggcag aagcagtaga taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgt
     5761 ggacaacatg ttttcaaagc aatcgcatca ggtgccgatc ttgttgctat tggcagaccc
     5821 gttatttatg gattgtcttt aggaggaagt acaggagtcc atcaagtgtt tgatttcttt
     5881 aaaacagaat tggaaatggt catgcaatta gctggaacac aaacagtaga agatatcaaa
     5941 aaaataaaat tgcgtgagaa tcgttttatt taagaaataa atgacgaaaa aaataggagt
     6001 gcggcagatg atgccgtact cctattttta tagttcaagg atgtcactaa tagaataatt
     6061 tgaaacgaaa aaataaaatt gaaatatttt ttcatactta ataaaaaagg aagaatattc
     6121 atgtaaacga ttttattgtt tttttctctt ttatacaaaa agggtcataa tttcaagtaa
     6181 gcggatgaat tcatgaggat tttttgcata ctgtctctac tatacttagg tcactaagaa
     6241 aaaggagtga tttattatga aaaagaacat gattgggaca ttattacttt caacacttct
     6301 tctaggcggc ttggcaacac cagcttttgc tgagggtcaa gcgacttcaa aaggagatat
     6361 tacctttact gaaccaacaa ataccgttga gcctcttaac cctactgatc cttcaaaacc
     6421 agtagggcca gcagatccgg aaaatccagc aactggccag acaggttctt taacattgga
     6481 tgtagttcct gaattgccat ttggtacaca cgagattgaa agcggaacaa aaacgtatca
     6541 agtagacgct tcaaaaaacg atacacctta tctccaagta tcagatcgtc gaggtgtagg
     6601 tgcagatggc caagcacaag ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg
     6661 ttcacaagtt ttgcaaggcg ctgaattaga ttttggaaca tccactgtta aaagtacgtc
     6721 agataacgaa tccactggac caactagtca aactgtaact gggttgagta aagcaagtgc
     6781 agcgacacca atatttacag cagcaaaaga tcaaggtctg ggaacatggc tttctgttta
     6841 tgatccagca aatatcacac tgaaagtgcc gaaagcagca gctggaacat tcacggctga
     6901 tttgacttgg aacctcgtgg ctggcccagt agcatagagt atgaaataaa ttcaacaaac
     6961 tgcggtgaaa atactatagg tgaaggattt tttaccgcag ttgttgttga aaggatgtgg
     7021 tggacaatga atcaaagtaa acgactgttt gtttcgtttt tttctatttt aagtctattt
     7081 ttcatttttc cgtcaatcag ccaagcaaag gattcagcag gagactttgg aatcaaaccg
     7141 gtatttccag agaaccaaat agataaagca atcggttatt ttgatttatt agtggcaccg
     7201 gaacaaaatc agacactaga agtaattatc agcaattctt cagatgaaga acgaacattt
     7261 gaggtctctg tcaatccggc ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa
     7321 aacccaacgt tagatgagac attgccgttt gatgtccgtg acgttttgct cattgcgaaa
     7381 aaggaaatca atgtatcagc acatgcagaa acaactatac ctatagaagt gaaaatccca
     7441 gcaaaaagct ttaagggacg tgtattagcg ggtattcatg tttcacctaa agaagaagct
     7501 gaaacagaaa atgctacaga aggagcacaa atcaaaaatc gtattgctta taatctcgca
     7561 gtcgttttac aagaaagtca agagacgata gaaccggatc tgaaactttt atcaggagat
     7621 ttggacgagg tcaacgcaaa gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg
     7681 attatcagca atttgatatt tacgagcaag atcttttatg aaaatcagct ttatattgaa
     7741 aatacgtcta atgaattttt agttgcacca aattcgaatt ttcacttgaa tcttgattta
     7801 gctggtgata aagcaaaagc cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac
     7861 agtaatgaat ggcgatttac acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta
     7921 aatgaaaatt ccgtttttgc agtacaagaa caatctttcc catggcttta tgcaggattg
     7981 gttgctgcag ttctagtgtt atggttgatt ttgctgtttt tgtgggtcca caagaaaaag
     8041 aaaaaaggag aaacaacatg atgaggtgat ttcgttgaaa acatcgatct tattgggagg
     8101 gattattgca agtttgctgt tcttcagctg gcaagagcca gtccaagcag ataccaaaag
     8161 ttcaggtacg attacatttt atgatctaag tccagttccg catccaagtg gttcagtcaa
     8221 tggcgcatca aaacagcaga cacagctacc gatttataaa gaatctaatc aacatggcgt
     8281 attcccggag acgaatgaag aaaagatccg ttttctttat ttgatcattg gtatagaatt
     8341 atggcttctt gcgtggttgt tgttatggag aagaaaggaa aaggaggatg aacatgttgc
     8401 tacaaaataa attttcattt tttcgctcct tcttttttac ttcgcttttt gttttaagta
     8461 tatttatctt ttcaccttcg gcagaagctg ttggagcaac gcctcctgcc atttcttatg
     8521 atccatacac gtatgaaaat atcgtacctc caaatggtgg acgtagtgca gtcaatggcg
     8581 ggaagcttgt ccaactaact ggggctaacc aattgcctaa attgactgct acttatttga
     8641 acgatggttc aaatgatcct tggaaaggtg gcacggtccg ttttcattgg gaaagccatg
     8701 tgaaactaac aaatgtactt gatttggtga atatcactgc acaaggattt gcatttgtgg
     8761 cgatcatcaa tttgccggaa catgtatcaa gcagtgatgt attaaaagca attgactgga
     8821 acaacgccta cttgtatatt gccggtgccc ccatcaaggt tactagcggt agtttattat
     8881 cagcaggaag ccataccttg cgttttggga tggggtcgtg ggatacaaat actctattgg
     8941 cagatattat tgctattatt acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat
     9001 ttgatgttac agctaatgtc gcgaagatga cagaaaacgg agataaatat gatacgagtc
     9061 caaacaaaat tttgaccaag ggtaaattac aaccttctat gtctaaaaag gccgtttttt
     9121 ctgttgattt ttatgattcg aataatatta tccaagatcg tatgttcaat tcgattttag
     9181 ctccagctgc aggtagattt tatcctaaat taactggcgg aaacttgaat ccaggagcat
     9241 atgcattttc tgcaagtgca gagataaata cgtggaatag ttacatctca acaagtgatt
     9301 caacaggaaa atataataca tttgctgata ccacagaata cgtggatgga aataaccaga
     9361 accttccagg ctctaccaat agtcggacat tgacgatggg gctgactgaa ggacgttttg
     9421 atcagttgcc atttagtcgc tttgatcgcg tagtcaacta ttttacagga aaaaacgtta
     9481 cacaaggagc aatactgact catactccag caagcacaag tggattgaat cagacagcca
     9541 atatcgtgta cagtgggaaa gatgcgcaag gcggattgct ttctcctgtt gctttaaagg
     9601 tattggagca gtacaagcta gacggaacgg ttttaccaac aaacttaaca gccgatagtc
     9661 agtggggaaa gctcctttct tctgcatcgt accaagtcac ggccaattgg tcagcaactg
     9721 aactaaaaaa gggttcgatc cattacaggc tttattcgaa aggaacccaa aaattagcga
     9781 caggatggac agatcaactt ttccaaacga tccaatcaaa taacgggggt gtaaatacag
     9841 ctacttctac actacctgca ttggcttcag ggcaatatta ttttgattat cgcttggtgg
     9901 acgatgtact tactaacatg tatccgtctt ttgcgtacaa atggcagtct gaacaagctg
     9961 gaatcaccac tcttcctcaa ataacagtgg ctaattttcc atctgtgagt gcagatagtc
    10021 agctaaaaaa ccttagcaga caacctgaga caggagatcc cctgtctgct ctatcaggag
    10081 atacgattca agaaaatgtg acattcacgt taacaaaatc tggggatgcg ataaccgaca
    10141 aaaaaatcac gatctcactt cctgaaaata ctacttatat caatggttcc ttaaaattaa
    10201 atggtacaac tatcgcagat accggcattc aacaaggagt ttctgtccca gcagatctat
    10261 tgagcaaaat aggagatacc attcatctta cttacaacta tcaattaaat actgtagata
    10321 cttctgtaca atccgtcagc atactgacaa aggcagctgt actttccagc aatattacat
    10381 tagcagatgg tgctaagctt cctaacccag ttgtccaaac atctgctaaa acaattcttg
    10441 tccccaaaca agaattaaca ttagttaatg taccagatga ttttacattt ggtaacgatc
    10501 tccccaaacc gttgaagaca agttattatg aagcaaaagg cgatttttct tttgatgtgc
    10561 gggatactcg gcttccaagc acgtctcctt ggcaactgac agggacattg acttctttat
    10621 ttaagaatga tcaaggtcaa gaattaagcg gaacaaaatt atacttcaat cattctggat
    10681 caaaacaatt gattcagcaa ggtcaaaaca cgttgattta tgagagtgat ggaacagcta
    10741 aaggagaggt tctcgttgac tttcccgata ccgatggttt actcttagag gtaaatagca
    10801 gtacgaatgc tcaacctggc gcaacgtatc aaggcatggt cacatgggaa ctgacagctg
    10861 ggccaaccag ctaagtgatt agaatttgta tggatctgtg ataactgtca cagatcctct
    10921 ttttcaagga tgagaaggaa acttctgctg ttaaaatcag aaatgcttgg aggagtggac
    10981 ttggaacttt tcagtttgct tgaaaaaaat gaacaacggc aaatcaaact tttccaactt
    11041 ttattagagg aaaaaggaga gatttccgtt aataacgtac ttacccgctt atcagttgat
    11101 cgggctacct tgaaagaaga tctgttttat ttacaagcta acttggaaaa tatcaaacaa
    11161 ttggaaattg ttatccaaga agggaatatt ttattaatca gacatggaaa tatagcagca
    11221 gtcgatgttt attatcattg ttacttgcgc tattctacaa aatatcagat acttctttat
    11281 ctgttggaaa atggctcatt tgatcggcag aaacttgtca tggaacttaa tatcagttcg
    11341 gctactttgt ctcgcagagt gaaggaatta aatgattctt taaatgagtt tcatctgaaa
    11401 ataaaaagcg ggcggctgac cggtccagaa agtcaaattc gttattttta tttccaatta
    11461 ttttggtttg gacgtcccta tcttgaaaac aaaaaagaat acagggattt atcaggaaac
    11521 acttttttag atctgctgca aaaagagttt agttttgcta ttacagaaga aggacgattg
    11581 aaatttgaaa tatggctttc cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa
    11641 ccagattata gcaatcttcc tgttggattg tttcaaacga cacaattttt attggacctc
    11701 catacatttt tgacccgtta cttctttcaa tatgcctttc cttggaatca aaatgaaacg
    11761 ttggtttttt atctcttcat gatcagtaac tttacattag atacacataa ctcgcatgtc
    11821 caggagtttt tgataagcga aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg
    11881 tttaaacaat cgatagaaga atatttctct ttttatgttt ttccagaaga gatttcgcaa
    11941 aagattgcgt tgacagtgat gcaaaaccac gctcattttg cctattttag aggatggatg
    12001 aatatttttg gccaagaagg attaaaacag cggttaactg aagtagaaga taaacaattc
    12061 ctggttttat gtgaaagatt ggtcagacaa acatttgcta ttgttgattt tactgaagta
    12121 gacgaggaaa atgcccgctt tgaattattt gctcgctatg catctatttt tcggacgctg
    12181 atcaagcagt tagaattacg tctgcaaatt gcttatgatc ttccttatga aaggctaatg
    12241 gcggatatcg tgaaagaaat catccaggaa caattgctga atacgttcaa atatgattta
    12301 gtcgaatacg agaaagaaaa gcagtatgat atcattttga ctagccaatt gaaagagtac
    12361 ccgtcacaga agtccgctaa agtttttgta ttcactagca acgaatataa ttatgatttc
    12421 ccctatttga accaattttt aaaagaatgt tatttagaaa aattgaattt aaggatgaat
    12481 aaaacatgaa aatcgtgatc attggtgctt cccatgcagg catcacagct gccctaaatc
    12541 taagaaaact tcaaccgaaa gcagaagttt tactgatcga tgaagatcat aaagatggac
    12601 taggctatgt ttcaaatgga atcaaccttt atttaaaagg aaagatacgt agtttagctg
    12661 aagttgccca taacatgcgg actcttcaag aaagtggggc aaaactgatc accgaatgga
    12721 gagtaactga acttgatcca gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa
    12781 acgagaccat aacctatgat aaattaatcg tagcgacagg ttcctctcct gtcaccttgt
    12841 ataaacaaat tgaagctgaa aatgtctaca cctataaaaa tctagtgcaa tctaagcaag
    12901 tacttgccgc tttaaaagaa gcaaaagaag tagtgatctt cggtgctggt tacattggat
    12961 tggaattagc agatgctttg agaaataaag gatacattat tcatctagtt gattatatgc
    13021 cgaatgtttt atcaagatat ttcgataagg atatgatcaa cagttttcaa aatcagctgc
    13081 aaactaagca aataaatttt tatccaaatg aattcttgat cgattggaaa aaatcagaag
    13141 agaaagtagt atccgtacaa cttctgtctc aaacaataaa ggcagatatg gtcatttttt
    13201 ctgcccaaac tcgtcctaat acgacattgt taaaagaaaa agtggctttg tatgaggatg
    13261 agactgtgat agtcaatgaa tatctgcaga caagtgatcc ggacatctat gcgattggag
    13321 atatcgtccc agtcagtttt gacaaaaata aacggcatct ctttttgcct ttagttactc
    13381 gagctgtaca tatggcaaga gcagtagcgc taactttgag tggtcagcca accgcttacg
    13441 acctcagaca aaagataaca gcagcagtca ttatagacta ctttttagga actgtagggc
    13501 tgacagaaga tgaagcgcca ttcttggaac aaaataccgg ttcgtgttca ggagaatttg
    13561 atctatttcc acaatacgat gaagaaaata aaacagtaaa tgcaaagctg atctaccatc
    13621 cggatacatt agaaataatt ggcggacagc tgataagcca agaattctta ttatcagatt
    13681 tgaacctgct tgcggatatc gtgaaacaca aaactacgat tccacagcta gcagttgagg
    13741 attttggatt tctgtctgaa tatacccctc gttttcacta ccttaatgag ttagctttta
    13801 aagtactagc taaaagagca aatagcaata gagtagataa aagactgtga gaaatatcac
    13861 ggtcttttat tgtgtgggga tataataaat attaatcaat cgagaataaa aaaaattcat
    13921 ataatgaaat agcggaagaa ttttttgagc ttaatattcg ccaaaaattt atcaagtgat
    13981 aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat
    14041 gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgattgcc
    14101 tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca
    14161 gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg
    14221 atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt
    14281 gcccaaacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt
    14341 agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct
    14401 tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc
    14461 gtaggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg
    14521 gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagag
    14581 atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga
    14641 ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat
    14701 gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc
    14761 gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat
    14821 atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg
    14881 gcacaaacat ttattttatc atttgttggt attcttcttg gtctattagg aacagtggga
    14941 acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc
    15001 attagcttgt tgatgctggt agtagctgtg ttaggcgcac tattttctgt tcggacaatc
    15061 gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagaacatg aaagcaatag
    15121 aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag
    15181 aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag
    15241 gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat
    15301 ttatcaatga tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa ctacgtttta
    15361 aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc
    15421 agttattatt agtagataag atcaaacatg aacatcgtca aaaagaagca aacgaattat
    15481 ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag
    15541 aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag
    15601 atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa
    15661 aagaatcaaa agacaaaaat aaagcaacaa tcatggttac tcacgataca cggctgattg
    15721 acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa
    15781 aaagcaggtt gaaaaatgat ccatcgatac aacaaagata cacagcaaaa aattttgaaa
    15841 gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag agagatcgca
    15901 caaaaagttg ggatcaccca gcccaatctc tatcattatt tcggtgataa agaaaaacta
    15961 tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt
    16021 caaacaagcg aaacgggatt tcaagtgacc ttgacaaaaa tggcgcagtt tttgattgag
    16081 acacatctga tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa
    16141 acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc
    16201 ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat
    16261 tttttcttag tgttagcacc ttacatcacc cggtcgaaaa gacctgtgga agaaccgcta
    16321 aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt
    16381 tatataggga gaaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt
    16441 gcttttttat ataggtagtt tttcataaat acaaatttgt tattctctca tgattatcag
    16501 ggtatatttt tatttattat gtcggtgttc caggaagtgt tttaatttaa taacgaagaa
    16561 attaattgtt tgtagaatga aggaaactat ttattgggta gatgaatcac cacgaatagt
    16621 caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc attttagcag taatgttatt
    16681 ttctacggtt atatctttta cagccccttt tttgcgtact taagctgatt ttaagttttg
    16741 gcccttagtc tggatctacg aggtgagagt caagaatgaa aaaaataatt ggagcttttg
    16801 tatttgcaat cattatcatt attatctgtt ttcaaataga tgtttttggt caaaaaaaga
    16861 tagttggaca aacggtcagc agcacgaaca gtacggttac aaaggcaaag gaatcatctg
    16921 atgacacaga acaagagaca ttagtcaaaa tcggaaaaaa tgaccgtaca ctatatacag
    16981 atgactcgct aactacccca cttgcaacta taaacggcgg agagctgacg gagtttcttt
    17041 ctgaaacaaa aaatgcttat caaatcaaga caaatgatgg ctatactgga tatttagccc
    17101 tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa accggaagag ctatctgatg
    17161 cagtgattgt attagatcct gggcatggag gaaatgatac tggggcatta agcaatgatg
    17221 aacaaacaga agaaaaagat ataacattaa gtacagccat aaaagtaaaa aaggtgctag
    17281 aggatgctgg agcaacggtt tatttgaccc atactacaga tgaattagta cagcttggag
    17341 atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta cacgcagact
    17401 ctacggaata tgcgaatgaa gcaactggaa tcacgacata ttattactat ggacaagagg
    17461 aaacattagc acagacaatt gctgactcct tcactgatct gcctctggat tcaagaggaa
    17521 tctccactgg gaactatcaa gttcttcgag aaaatcttca accttctatt ttaatcgaga
    17581 tgggttatat gaataatgat agtgatttaa aggaattagt aacagacagt tatcagcagc
    17641 agattgctgc aagtttgaca caagcattga ccacttattt tcagtaataa ataagcccag
    17701 ccaaacaaaa atagctcctc atatttttcg tttggccggg cttattatcg cagatgcaag
    17761 cttttgaaca ccgtttattt ttttagtagc ttcaaagctt tttctgtatt tgcaaagtga
    17821 agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt tgccgctact
    17881 tggtcagatt tcgttccagc tcctgatgga agggtgattc cagcttcagc tgatgctttg
    17941 tgtaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac agctaaatga
    18001 tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt ttctaaacgc
    18061 gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc ttctggtttt
    18121 tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc
    18181 cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa gacaagcagt
    18241 ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct atcttttaac
    18301 tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg tttcacataa
    18361 gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc actacctaaa
    18421 acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg cttggcttgt
    18481 ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc ttgaaacatc
    18541 acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc aaagctagta
    18601 taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg actaagttgg
    18661 tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt atacataaaa
    18721 ctataccgca actcacgact tttcgttttc taagagattg cgagacatag cattttttta
    18781 aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg tgaaagaacc
    18841 aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt cgtatggcgc
    18901 acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt atcggacggg
    18961 aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta gcagaattga
    19021 aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct gtcaatgcgc
    19081 tttcggatca attgaagaaa caagaaagaa tcttggagct ggaagaagaa acagcagaat
    19141 taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa
    19201 tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat accattaaaa
    19261 atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt aaacaaaaat
    19321 cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaaagtta gaagagaaag
    19381 gaacgtagca tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga
    19441 gcacgtcgcg ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt
    19501 gctgcccagc atttttacaa accattagca agtcatttgg aattatacat tccataccca
    19561 gctgtcacgc caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat
    19621 gaagcttttt atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc
    19681 cgatttgtgg gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac
    19741 tggattgcag gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg
    19801 cagttacttg cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc
    19861 gctcgcttta tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta
    19921 acgaatattt tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta
    19981 gattatttaa gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga
    20041 taaggtgata aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag
    20101 atggtcagac aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca
    20161 gtgagcgagg aacaaacgtt cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg
    20221 caacgtctgc gaggcgggat ccctatacct aaacttgaaa atatccgtcc ccatatgaaa
    20281 cggattgaga tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg
    20341 tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt
    20401 gcacgtttgt atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta
    20461 aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc
    20521 ataagacaga acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc
    20581 cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac
    20641 gttatcccag tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt
    20701 gcttcaggac agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg
    20761 cgtcaatacc aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct
    20821 gaacttgttc cccatcgtca agaaatcatc cacaatgctt atgtgattgg taaaatggat
    20881 ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag ctatcgtgcc gggaatcagt
    20941 gaagacaatc atgtcgtctt gaaacaagca agacatccac tgatcgatca gaaaaaagta
    21001 gtttctaatg acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac
    21061 acaggtggga aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca
    21121 ggattaccga ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg
    21181 gatataggag acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg
    21241 aacactgtag atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc
    21301 ggtgcaggaa cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt
    21361 ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga
    21421 tataatcggg caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca
    21481 acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga
    21541 ttaggtcttg atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa
    21601 gatttaaacg aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat
    21661 ctggaaatgc gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct
    21721 tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat
    21781 gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg
    21841 caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa
    21901 ttagcaaatc tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa
    21961 gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg
    22021 acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat
    22081 gtgtccgaag acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc
    22141 catgctgtac gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa
    22201 cgatacgaag aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt
    22261 tatcctcaag tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact
    22321 gattatctta aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc
    22381 ggaaatggtg cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc
    22441 gctatccaag tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata
    22501 tgtcacaagt aattacagat aagacattta atgaagaaac agataaagga cttgtattga
    22561 ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt
    22621 tggaacaaga atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc
    22681 cagaaactcc tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg
    22741 gtcaagtagt agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg
    22801 atcaatattt ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc
    22861 gtcctcggtt tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat
    22921 tacgttgtca ggctggattg tttttttgac agacatgcta taataacttt aattgaatat
    22981 gaaggggagt accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag
    23041 cccggctgtt acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa
    23101 atattaaaaa atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg
    23161 cttgttagtg aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg
    23221 ctcttagaga tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat
    23281 gataaaacct atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact
    23341 cgtaaggtac taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg
    23401 aatgagacaa agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag
    23461 acgaaggagc cttgtcttta ctatcatttg ggacaatgcc tgtgtcctta ttatttcgac
    23521 gtagatccga aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga
    23581 cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag
    23641 tttgaaaaag ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc
    23701 caaaaaatga caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa
    23761 ggatggatgt gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt
    23821 tcaatgtttc ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt
    23881 tatcaggaaa atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag
    23941 cccagtgtag aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa
    24001 aaattagttg aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg
    24061 attgccagaa aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat
    24121 atcccgaccc caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct
    24181 gtggctgcga tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat
    24241 aaaataaaaa cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc
    24301 agatattcgc gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga
    24361 ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt
    24421 cctattgctg gcatggcaaa aaacgataaa cataaaacaa atgagttgct attcggtaca
    24481 gatttggctg tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc
    24541 caagatgaag tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt
    24601 tttgcttccc gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt
    24661 aaagagttca aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc
    24721 ggactgccga aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag
    24781 ggcactttac aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta
    24841 cgttttgttg gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct
    24901 tgaagaagga gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac
    24961 acagctgtcg atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc
    25021 caaaacgggg ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac
    25081 caaggagaag tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt
    25141 tatcttccgg aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat
    25201 tttgcagaac ttagagggaa atcaagaaaa gagattgaac caaaaatcga tttttggatg
    25261 gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat
    25321 cagcaaaaag tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat
    25381 gagccattca gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa
    25441 ttgaaaaaac atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa
    25501 atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat
    25561 gagattcgtg aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag
    25621 gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag
    25681 attaccttag aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc
    25741 tacattccta tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca
    25801 ggtgaaatcg atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa
    25861 gtcgatttct ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt
    25921 agcaggaaac tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa
    25981 tgagcaaatc agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt
    26041 tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt
    26101 agtctcaact gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca
    26161 aagtacacaa ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcaag
    26221 tcaattaggc atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg
    26281 acaaaaagtc aattttgatt caaaaggaaa aatgacagtt ggagaagata actcgacagt
    26341 ccaatatgtc gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc
    26401 gcagatcatt gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct
    26461 gtccagtacg actgcacaaa agcattttta cggaaaacta acaggtgttt tacttgttgc
    26521 attaacgcaa atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga
    26581 tatggaaatg gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt
    26641 cttgtggtat tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc
    26701 tttatgtggc tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac
    26761 ttatctatct ctaggcggat atatgcttgg attgatacta ggagcttcag atccaaacaa
    26821 tatcgttatt cggatcactt cgtatattcc attcttatct tcatatatta tgccggttcg
    26881 cttagcaaat gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc
    26941 cgtaacattc ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgtgta
    27001 tagcgaaggc ggattatgga gttcattgaa acaatcgatt tcaattatga gaaatgaacg
    27061 aaaaaaagca taaaatatag aaaaatatag aaggaagctg gacatttatt gttcccagct
    27121 tcttttttgt atagacctat ttctattttt ttattatatt ggatatatat tcattgaaaa
    27181 tagagcataa caactttata atgttgagta taccaatttt tggaggcgtt ttaatggacc
    27241 gctatttaga tttactttca gagaagttcc ctacaacgga agatgtcatc acggaaatca
    27301 tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg agcgatatac
    27361 atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa
    27421 agatcaaaga ccttttctct gaacaaatga cggaaacgga gatcagccaa ttcactattt
    27481 ttacggctta tccagaatat gtgttgacga ctgattggta caaaaaacaa gataaatccc
    27541 agctgatcca tcgtttgatc gatcttttac ggtttacgac agtcaaatac actcgatcaa
    27601 aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg
    27661 atgatcgtat caacgggaaa aaagactatg taaaaaaaat cattgaacaa cttgtcttga
    27721 tgaaggaaga agaaaaattt ttaacaaaac ttgcgcagac gatccaaaaa tctgtcatcg
    27781 atcatttgca tatcgtgggt gatatttttg atcgaggaac tcaagcagcc aaagtaatgg
    27841 atcgaatcat ggatttttcc tctgtagata tccagtgggg gaatcatgat attttgtgga
    27901 ttggtgcgta ttgcggctct gaggcatgtt tattgacgct tttacggatt gccgcacgct
    27961 acaactattt atttgaatta gagaaagaat atggattaaa tttacgtcca ttattttctt
    28021 ttgcccatca aacctatcag aagaatccag tatttacccc taaaaacagg aaagatctat
    28081 ctgaaagaga gcaagaagaa ttagaaaaga tccatcaagc actttctatc ctccaattca
    28141 aatcagaaca tcagttgttg aatcgacgac ctgaatttaa aatggacgat cgtaaattgt
    28201 tggaaaaaat caactatgag gaatcaacga tcgttttaga aggtcagctc tatgagttga
    28261 aagggacttg ttttcaaacg atccagccag atgatcctaa aaaattcttg gaagaggaac
    28321 aaaaagtgat cgatagtttg atgtattcat tccagcactc gttaagaatg caaaaacaca
    28381 tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtactgttcc
    28441 atggctgtat acctgtagat gaaaaggggc agctcttagc atttgaatta gaccaatctt
    28501 atcgcggaaa agaactgctg ggattttttg aaaaacagat aactgaaagc gcaaaagatt
    28561 tgactgcaaa tgaagatttg gcgaccgatt tgatttggta tgcgtggagc gggccatttt
    28621 cgcctttatt cggcaagagt aaaatggcaa cttttgagcg ctattttctc acagagcctc
    28681 atacgcacgt cgaaaaaagc aatccctatt atcacttgcg agatgaagag tggttcagtg
    28741 aaaaaatttt ggcagaattc gatgcaaaag aagaaggttc agcaatcatc aatggacaca
    28801 cgccagtaaa agtaaaaaaa ggagagtcac cgatcaaagc aaacggaaaa gcatttgtta
    28861 ttgacggagg tctatccaaa gcttatcaaa aaacaaccgg gattgctggc tactcattac
    28921 tatgcaattc ttacggtttc caaatcgtga cacattatcc gttcttgcct attgaacaac
    28981 aatttgcaaa aaaatctgat caaacgaatg taaaaaaagt gatcgagcag cagctcccac
    29041 gcaaacttaa tcggaatacg acaaaaggaa tggaattaca gcaacagatt actcaactcc
    29101 aaaggctgtt ggattatcga aaggacgatt gagaaaaatg ctagccaaca ttgctcaata
    29161 gactaaaaag cgactgcaac aaaaggcgtg tcgcagtctc cttcggaatt agctcaaaaa
    29221 acggtaaaat attagaagct atttttctgt ttatttctct aatactcagg tcagggcgct
    29281 gatttcacga cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    29341 atatccaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttgaac
    29401 ggcaggctca cgacctaatc agaaaaacga ttaaaattca ttcacaaaga aatcgaataa
    29461 acgctgttca gatgaatcta cacgatgaga aagttccgga tgccattgga caccaaggat
    29521 tttgtgcata ttatctttac tttggactgc ttcaatgagt ccatcgttgg aaaaggcgat
    29581 ggcttttaac aaaggagaaa gatctttgac tgcctgatga tgataggaat tcacaaagta
    29641 tgaatcaggg agaaggtcac ttaataaact gtccgaaacg attttgactt cgtgagttgc
    29701 aaactgcggg gcagtgggct gttgttcgtg ttttacggac cattttggat ataatgaaag
    29761 atcttgataa agtgttccct ccaacacgac attcaatagt tgaagtcctc ggcagactgc
    29821 aaaaatcggt tttctttgtt tcaatgcttc caagatcaat gcctgttcga attggtctcg
    29881 ttgaatattt gtttccgtca gttttggatg agggtcttcc atgtaaagtt gcggactaac
    29941 atcctgaccg cctgcaagaa tgagtttgtc gattttctca attagttgag gcgctgaatc
    30001 tggagacatg actggcagaa taagaggtaa tccttgagca tctaaaactg catccacaaa
    30061 tccctgcggt gtatagctta cttgatttcc ttggaaagta tctgtcgctc gaatcagttg
    30121 attcccggca atgccgatga ttggtttcat ttttattccc cctaaagtag actaattgta
    30181 acaaggattt aaaaaaattt aaactatgta agacttgccc ctatacttat ctgttcttgc
    30241 tgtgttacta tctgtaaaga gaattttcgt gtgaaattca tgacgatagt tgaaaatatt
    30301 gcttaatttg ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg
    30361 atctagttct tcaaggggtg agaaggacat tttattgaag gggcctacct atactaagtt
    30421 atctttagag gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga
    30481 aaggaagtga agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat
    30541 gagaagcgtg ctcgaaccct acggcagcag ttgaagacct atgcagaaga aaaagagcaa
    30601 gaattggaga taatccaaac gaacagtcgg caacagctga aacagttggt agaagaaaaa
    30661 gaacaattcg aaaagcaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac
    30721 aaggtgcaag caactttgaa agaccaatat caaaagcatc agagtcaagc aatcggtgca
    30781 atcattgaaa gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat
    30841 ttcagataaa agaaatcagg agaggatttt acaagcagta caaggaatcc aaaatgttga
    30901 aatccgggat ctgtatcaaa atagaacaaa caaccagtgg gtagaaaaat attttccaga
    30961 taccacgatt attgataaag aagcaaaatt aagcgcttta ggcaatcggc tgaaagacat
    31021 ccaagaagct atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt
    31081 gaaacgtcaa gaactttcct tacaagactt ggaagcaaat tattccgaag aaaatttcat
    31141 gaataaatta gaagaagtcc ttgatttaaa agaaaagtgg gaacagctaa ctgaaaaacg
    31201 tgatcgttta gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc
    31261 aagtatgtat gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt
    31321 tgaaagtttc aaggaagaac taagtaagct tgatgacgtt tatatggaag aaatagatta
    31381 tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgctataga
    31441 agaaattgct gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa
    31501 agaaaaattg caagaaacca aacagcaatt atccattgtt cacgagcagc aaaaaaagat
    31561 aacgacagcg ttaggtcagt gcagcggcta tatccaagaa tttgaatggg tagaagaagt
    31621 cacacttgcc gttgcagaac gggaaaaagt aaaagaacgg tttatccatg ccacttatct
    31681 gatcgttttg caaggttggg tagatgcaga agaaaaacag aatttgatgc atgtgttaaa
    31741 cgaaactgta tcagaagaag atatatacgt tagtttcgaa acaccaaact cagaagagat
    31801 tcagcaggaa gtccctacta aattgaaaaa ccatccgctt atcgaacctt ttgaattgct
    31861 gactgaaatg tacagtctgc caaaatatga agaagtagat ccaacgcctt ggatgacacc
    31921 attttatctt gtcttcttcg ggatgatggt cgcagatgtt ggttacggat tgctgatgct
    31981 cattgggaca atactagctc aaaaactatt tgttttaccc cgcgggatga aacgtttcgt
    32041 aaaattcttt gagattctct cggtaccatc gatcatctgg ggattgatct acagttcatt
    32101 tttcggacag gcattaccaa aagaactatt tggtatccgc ttgcctttcc caatcctatc
    32161 tacgacggat gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat
    32221 cttggttgga ttattcgtgg cagcaaagga aaatatcagg aggaaagatt acttgagtgc
    32281 tgtcagtgat ggttttgcct ggcaagggat cctgattggg atcgttattg cattgattgg
    32341 tgtcatgttg ataaaaaata cagcatttgt ttatcttggc gcggggctat cgatcattgc
    32401 tgctttatgt atcttaatcg tgccgattat ccaatcgccg tctaaagtca agggtgctgc
    32461 caaaggagcg tataatcttt acggattgac tggttatatc ggtgatttag tcagttatac
    32521 acgtttgatg gcactaggta tttcaggcgg aagtattggc gcggcattca atatgctagt
    32581 tgctttcatg ccgccagccg ctcgattcag tgtagggatc cttttgatca tcgctttaca
    32641 cgcactgaat atgtttttga cactgttaag tgcctatgtc catggtgcac gattgcagta
    32701 tgtcgaattt tttggaaaat tttatacagg aggcggacgt gcctttgacc cgctgaaaac
    32761 ggcggaaaaa tacgtcaaca tcaatcataa gaaaaaaaac aataaataat tggaggattt
    32821 ttatacaatg atggattact taattagtca aaatggtgga atggtatttg cagtattagc
    32881 gatggcaaca gcgacgattt tttcagggat tggttcagct aaaggtgttg gaatgacagg
    32941 tgaggcagca gcagcgttaa caacaagtca acccgaaaaa ttcggacagg ctttgattct
    33001 gcaattgctt ccaggtacgc aagggttgta tggattcgtt atcgcctttt tgatttttat
    33061 caacttagga aatgatatgt cagttgtaca aggattgaac tttctaggcg cttctctgcc
    33121 gattgctttt acaggtttgt tttcaggtat cgcccaaggg aaagttgcag cagcaggtat
    33181 ccaaatttta gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt
    33241 tgaaacatat gcgattttag gtttcgttat ttccttccta ttagtattga atgcctaagc
    33301 ataaaggagg aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaatgaagca
    33361 gcagagcaag aaagagcggc gttagagcaa gaagagcgaa tgaagatcga tcaaaacttt
    33421 gaacaaaaac gtacgcaaat cgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa
    33481 ttacttgaaa aaaaataccg tcagcttagg aatcgccaac aagtagaagt acgacaagaa
    33541 aatttgaatg cgaagcagga atttttacga cgattgtttg cagatgcaat agctgaaatg
    33601 gagaattggg atgaatctga acaaatccag tttatcaaaa attcattgta ttcattacct
    33661 ttgacaggca aagtaacatt cattgctgga gaaaaatcag cagcttatct ttctcagaca
    33721 ttgctggatg aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt
    33781 gctgatcaag cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc
    33841 agccttgtac aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa
    33901 taaggaggga tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc
    33961 ttgaactttt gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac
    34021 ttggtgaaat attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg
    34081 acttcgaaca gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag
    34141 caccggagcc tgaaatcgtt tggatctata cgatgcgcta tactttccat aatctgaagg
    34201 ttttgacaaa agccgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat
    34261 tttattcatt agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaactccctg
    34321 aaagcattat ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc
    34381 agggaattga cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata
    34441 agctaggata tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca
    34501 caacgatggc tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt
    34561 ccagctcggg aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag
    34621 attacacgaa ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg
    34681 agggaaatct tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt
    34741 atcaagaagc acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa
    34801 aagaggtaga aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaac
    34861 cagaaacaat cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc
    34921 gacaaagatt ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc
    34981 aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat
    35041 gtcacagagc aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg
    35101 atacctgcta tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa
    35161 attcaaaaaa acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata
    35221 gaaattttgc aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac
    35281 atgtctgatg ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa
    35341 attatcgaga tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt
    35401 ggtccaggcg aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg
    35461 atcatttcac aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca
    35521 caaagtaatt tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg
    35581 tggttcgaac caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc
    35641 gtagaagaaa caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggtacg
    35701 atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc
    35761 gaaaatggtg acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca
    35821 atcaaagaaa aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca
    35881 ttcttcccag tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa
    35941 acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga
    36001 tgtggcgagc gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac
    36061 cctaatacag gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg
    36121 ccagtagcag ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt
    36181 gacatgggtt atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt
    36241 cgtgagatga gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt
    36301 ggttcacggc ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa
    36361 ggtcgcgaag gaagtataac cgcaatcagt gctgtatcgc cgtctggagg ggatatttcg
    36421 gaaccagtca cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt
    36481 ttagcccaaa aaagacattt tccttcgatc gattggatac aaagctattc tctttattca
    36541 acagaagtag ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc
    36601 gagggcatgc gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg
    36661 attgattccc tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa
    36721 gattatttgc agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa
    36781 ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta
    36841 ggtgcttatt taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga
    36901 agcaaatata tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag
    36961 caaacgatgc aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac
    37021 gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga
    37081 agaattaatc gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt
    37141 ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga
    37201 ctcttctgta cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg
    37261 tcgagtattc gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa
    37321 gcatttggat attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt
    37381 tatccaaaca ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt
    37441 accagtattt tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca
    37501 agcaacggta ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac
    37561 ttttgaagaa gcagaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc
    37621 tgtcatgttt atgaatttag caaacgaccc tgcaatcgag cgtatcgcta ctcctcgtat
    37681 ggctttgact gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat
    37741 gacagacatg accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt
    37801 tccaggaaga agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg
    37861 agcaggaaga atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc
    37921 agaagacgat aaaactcatc caatccctga cttgactggt tatattacag aaggacagat
    37981 catcttgact agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc
    38041 tctttcgcgg ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc
    38101 tacgatgaat cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattggccgt
    38161 agttttaggt gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg
    38221 gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact
    38281 tgatctaggg tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga
    38341 cgacatgctg gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt
    38401 gaatccgacc cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg
    38461 gcataaatta ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg
    38521 aaagaacaac cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt
    38581 tgtattggca aatgcttcga tcacggagcc tttcatcgaa gaattatttg ttttgccggc
    38641 ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat
    38701 gaattttgaa tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc
    38761 cagtgccgaa ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga
    38821 actgacagaa atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg
    38881 gcgagtcaat gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat
    38941 tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa
    39001 tatgggtacc actgactaag tggtatcctt ttttaaaaga ggaaaacgat gattaaacaa
    39061 agcaaacaaa taaagaaacg cttgccggct gttcagcttc tcgcattagg attttttctc
    39121 ctgattttag ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg
    39181 acaaatttta tcgatgcgct tttcactgca acttctgctg tctgtgtgac gggattgaca
    39241 acattgaata cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt
    39301 gagatcggcg gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag
    39361 aaagtcagtc tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca
    39421 ggtgtgatga agctcatgct ttacatcctg aaattggcct ttgtgatcca actgattggt
    39481 acggttgctt tatcttttgt attcgtgcct gaatttggct ggttgaaagg tatctggtac
    39541 agtttgttcc atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt
    39601 ttagccaatc accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct
    39661 ggtgggttag gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagata
    39721 acgattcatt caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt
    39781 gtgttctttt tcacagagcg caatggggaa acgctagtgg aagggaatat gtttcaacga
    39841 ttggcaaaca ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat
    39901 tacctccata tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggtgga
    39961 acatctgggt cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag
    40021 atgcgttcga tgcttaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa
    40081 ggcgcagtgt tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc
    40141 atcatgattt tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct
    40201 tttgaggtct tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg
    40261 actgcaatag ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg
    40321 actgtggtct tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca
    40381 gaagaaagtg tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa
    40441 ataagaaaat aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt
    40501 attttttgag gcactatccg ttgttgaacc tcgggtagtg cctctttctt attgaaagga
    40561 atcaataaga tttgattcct ttatttatca ataggttata attcatattt ttttattttt
    40621 tcttttacga tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt
    40681 cgaacgaatg catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata
    40741 ccgttagcaa gaaagtcatt ctcaattatc ttaggatcaa tctcaggatc aactggaaga
    40801 attcgatagt aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc
    40861 tgattggctc ttttcgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa
    40921 tggaggattt gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa
    40981 attgcttgtt ctatcttgtt ttggacatgt actggaacta caagataagc aactcgcaat
    41041 ccagaacaaa taaattttgt cattccagct aagtagattg tttggttagg taataatttt
    41101 tggaaaggag gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct
    41161 tcaataaccc atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga
    41221 gagatttgaa accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc
    41281 ttttttctac attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt
    41341 ggaacgatct ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg
    41401 tctgttgcta ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt
    41461 tgaacaccgg aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac
    41521 caatttgctg cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa
    41581 ccaacaagat ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat
    41641 tcttcaaaag aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag
    41701 atggtgatgg actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac
    41761 agcaaacctt tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct
    41821 aaataatcag ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt
    41881 aaaatatctt gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt
    41941 ttgtctggtc gccaagttaa cgggtaattg tcataagaat taataggcat tttctcaact
    42001 ccttaaaatt gtataccata caattttacc attgattgta ttgattttag aagttaatct
    42061 tttgtgagga gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt
    42121 agttttgaag aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa
    42181 atcagccaaa atcctcgatt ggtggaaatt ttacctgacg gaaaagacaa aaatatgttt
    42241 gcgatgatgc ctgcttattt gggggagaac agatattttg gaacaaaagt cattaccgct
    42301 tttccggata atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat
    42361 gctcaaaacg gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca
    42421 gctgctgtat ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct
    42481 ttaattggag ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc
    42541 atcactacag tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa
    42601 gctaaaaaac agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta
    42661 cgtccagcgg aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag
    42721 tgggtaaggg aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag
    42781 ataagtacat cgttgatgga aacttctcaa atctatgtag atgattactc agctacctta
    42841 acagaaagcg gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt
    42901 gttggtacat taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg
    42961 ccgacgattt tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt
    43021 tataacaaat tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat
    43081 ttgcagatga ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc
    43141 cagaaggtac gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag
    43201 tgaagaaaaa ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt
    43261 gtggtcacgc gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg
    43321 aaacgattaa attttctact caaagcggta atctatttgt gacaagaatt aaagattatt
    43381 attacatgga ttttcctagt attatgccga aaaaagtgcc gatattgact gaatatgaag
    43441 aagcaattgg ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag
    43501 aagatgaaaa aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat
    43561 taggaataga gtcatcgtga cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt
    43621 ctttccaaaa ttaacaatca atgaagatcc agtatgtgga tcagcacatt ctaatcttat
    43681 tccttattgg gcagaaagaa tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag
    43741 gggcggggat ctagaatgtg aattaaaaga agatcgtgtg ataataggag gaaaagcagt
    43801 tttgttttct aaaggtacct cgtttatttg atttctttca aatgaattaa aaataaattg
    43861 gtggagagtg gcagccttat agctacaatg gttgttaaaa aaagacgcaa actttttcaa
    43921 tttaatgaaa agtttgcgtc tttttttaat tattggtgtt tgttttttat tcaatcaaga
    43981 gtaaaatgcc ccatactaac tatcctcgga tgacttcgat tttataacca tcagggtcaa
    44041 tgacaaagta gtaagaaggc gtagtgccag gtaatccttt taagtcagta atctcaagac
    44101 ctgcagcttt atgttgctcg tgtaattttt ctaaatcatc agctgcaata gcgatatgac
    44161 catagccgtc tcctagatca tacgcaccat gatcatagtt gtaagtcaac tctaattcat
    44221 agtcatctcc aggaagtgtc aaataaacaa gtgtaaattt gttttctgga aaatcacggc
    44281 ggcggctttc ttcaaaacca aatgcctttt gataaaattc gattgatgct tcaagatctt
    44341 tcacccgaac acatgtgtga gccattttca taaattcatc tctccttttc cttttactca
    44401 atcataacaa aaagaagtaa gaaaatagtt gatttgtgct tgttcatgag aaatttctca
    44461 tgtaaaatgg atagaaaata atactgttaa ggtggaaaca attcatggat aaaccaattt
    44521 ttggtgaaaa aaagcaatct gctaattatc aaacccgtta tgcagcatat attgtcatag
    44581 aaagagacaa gcggattgcg gtgatcgagg ctccaaatgg tgcttttttc cttcctggtg
    44641 gggaaatcga aggagaagag acgaaagaag aagcgattgt ccgtgagctg atagaagaaa
    44701 tgggaattgt ggcagaaatt tcttcttatc tgggagaagc tgatgagtat ttttactcga
    44761 attatcgtgc aacttattat tataatccag gatatttctt tgttgctgat tcttggaagc
    44821 gtataggaga accaactgaa aagaccaata aaatttggtg ggtgagccct caagaagcgc
    44881 ttgtgaaatt gaaacgtggt agccaccgat gggcagtcga gagatggatc gaaaaaaata
    44941 aaaactgaaa gaaagcgagt agatcagctt tctctcagtt tttttattta ttacagtaca
    45001 tattttttga ttgcttcagc tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct
    45061 gcttctttga caagagaaac ggcatttccc attgctactc ctagaccagc atattcaatc
    45121 atggataagt cgttctcgtt atctccgata gccatgattt catcttgatc aataccaagg
    45181 tgctgtgcga gattcgcaac tgccgcacct ttacttgctt ctttatttag tacttcgaag
    45241 taatagggcg cacttttgac cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca
    45301 attgctgcat ctaaaatttc tggttcgtcg atcatcatca ttttgacgat gttcatgtct
    45361 ggtgtcattt cttcaggtgt tcgatatttt agcggcatct tgactaaact tgcttcgtgg
    45421 atcgtataag cgctgatatc tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg
    45481 agatgactgc cgacacggcg tgccatcact tcgatctcta ggaaatcttc atgagtcagt
    45541 ccgtgacggg cgatgatttt acctgatttc gtttgttgta cgagtgcccc gttataagta
    45601 ataacgtaat cattatcttg aaaaaggttc aactcgttta actgttcctg gacacctggt
    45661 aagggacggc cagtgcataa aacgatcgaa acaccttttg caacagcagc ggcaatcgct
    45721 tcttttactt cttttgttac ttctcttttc tcatttaata aagtaccatc gatgtcgata
    45781 gcgactagtt tgatagacat aatttcctcc aatatatttt aatttatttc aatcaatgcg
    45841 ccattacgga tatggctggc aaattcttga taagtttcgt caaataaatc ataatgatca
    45901 cgcaaggagg gatcaaccat ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt
    45961 cccgctaatg cagcaacaag ctgactaacc tgtgataatt ctatcaaagt tccgtcattt
    46021 cgcaaaatct cgatttgtgt gcgatgtctg ccttgttctg ggcgataaaa atcataaggc
    46081 agatcgtagc tagaattcac agcagtgtaa taaacagggt tatagccaac cttttctact
    46141 aacaaagtga gttcttggac taattttgaa tcccggttgc cagaaaaagt agcagatttc
    46201 agtggtttac gattcaaaaa acgtttagct aaatcattta aaataggatc agcagattca
    46261 gaccattgag taaagtaagt gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa
    46321 gtaaagcttc cgtcaagaaa tggcagaagt aattgagaat ggagttcaaa actgctggca
    46381 tcttcttgat acaactcgtg tgcgcgatga agcagatgct ccaaaatcac ttccatacca
    46441 cgggaaacag gatggaagta cacttgcaca tacatttgat atcggctaac gatataatct
    46501 tccactgcat gcatgccact catggaaaaa gcgatcccgc cttcataagg acgaatcaca
    46561 cgtaatattc gggtaagatc aaatgttcca tattctgttc cagtgaaata agcatcccga
    46621 agcaaatagt ccattcgatc tgcatcgatc tgactagaaa tcatttggac aacttgtggg
    46681 ttggggtaag ttttttggat cacactagct accttttcag gaaaaccttc ttctactcgg
    46741 ttaagaatct gatacacttc tgtttctgga gaagtgatga tctctacagt gatggcttca
    46801 tgattcgtat ggaagatatg ttcaaatgta tgagagtaag gaccatgacc gacatcatgc
    46861 aacagtgccg cacataaagt caccagccgt tcctgatcat cccagcctcc gttgcctatt
    46921 ttttcttttg aaaaatttcg agaaaagata tcgcagattc gtcttgaaat ctcataaacg
    46981 cctaatgaat gggtaaaacg gctatgttct gctccgtgaa aagtgaaaga agaagtgcct
    47041 aattgtttaa tgcgtcgtaa acgttggact tctttcgaat tgatcaaatc taagattact
    47101 ttatgctgga catggatata gttatggact ggatcacgaa aaactttttc cataggcagc
    47161 atttgatatt tataaggggt tgtcataatg tagctccttt atgttattac gttgttccat
    47221 tctctggatt tggttgttcc attgctccag ctggatttct ttttttagcc agtcggtaga
    47281 atcctgtaaa tcacttgcag aatacagctt ttcaaaagct tttgcaaatg cttctttgac
    47341 ttcttttaca aaaaggggtt ggtcaagcag ttcttcaagt gttgccattg tagcaggatc
    47401 cactgcagga tagccatttg taccaaagtc ttcttttaaa gcagattgat aaaatcgctt
    47461 catgacttgc ccgcgttttt cctgattacc gttgacactt aagtacatca taacagaaat
    47521 cccatttttg atccgacgct gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc
    47581 ataagttcca ggacaataag aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa
    47641 agcgtattta gtcaatagga gcatttgctc gtaagcgtca tctatggtta ttttttttgt
    47701 gtcactttga gggaaaatca aagaaatatt taaaattcct tcatctgata cgatccctaa
    47761 tccacctgaa tttctgatca ctggagcgta acctgcatct ttcagtgttt ggatcccgtc
    47821 acttagaaaa ggagttcgaa catccttcat acctaagatg actgtgcagt catattgcca
    47881 gaaatgtaaa aagatttgct ggtgttcttt agaatattcc gttaatatat ctgttaatgc
    47941 aaaaggggaa aagtcgtttt tttgataaat gtgctgatct agtcgaatcg cagggttcat
    48001 tcaaccggct cctttgtata aattcatatt ttctacaata ttatactcta aaatcaaaga
    48061 gttgttttgg aaaaaaggtt ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa
    48121 actaggaaca atgtagctat gagaagtaaa ggagaatgaa taatggaatt atcacaagga
    48181 acaccgattt caatcaaatt gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt
    48241 tattttgatt tgaaagggca aatggtgaca attggcgata cgctgtacat acgttataaa
    48301 gagattcagg aggagacggg cgatgagatt cctgtaacga tcaaattagt tccagatggt
    48361 catgtacagc tgatccgtgc aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa
    48421 cggttagaaa catcctatcg aacaccatac ggtatgatcc agattgctac ttttacgaaa
    48481 gaactgcatg ttagtttgaa agaccgtccc acagcaggga aggttcgaat cgattatgat
    48541 ctgttcatgg gaccggaaaa aatcggcgaa tattatttga ccttggattt tacagcgtaa
    48601 atataaaaga aaagtttgat tttttgtgtc tgattttgta tgataaggag tagactgtga
    48661 aaggacgtgt cctgattgga aattagtgta tttgaaggtg caaacaaaag cgaattatca
    48721 atgatcgaag tagcacacgc gatcttagaa caacgtggcg atgtcatgga tttttctgat
    48781 ttagctaacc agatccaaaa ctatcttgag aaatcagaca gtgaaattcg tgattcactc
    48841 gcacaatttt atactgactt gaatattgac ggcagcttta tctcattagg ggataatcgt
    48901 tggggcttgc gttcatggta tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg
    48961 gacgaagagg atgaagatac tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc
    49021 aatgatgatg aagacgcgat cgattacaat gacgatgacc cagaagatac agatctgaca
    49081 gaagaagatg acgatgatct gtttgatgac gatgatgatg aagatgaaga aatcgctgct
    49141 tataattctg acttgcaaga aatcggagca gatgacccga cagatgatga agaagacttg
    49201 ccaggaaata tcgaagaaga tttaagtatc atagacgaag acgatgatga tgaagattat
    49261 gaagaggaag aattttctga ctctgaaaaa gaataaacat tattttctta cgagaggttg
    49321 tgacagagac gatcagccta tcagaataag gaggtgtgac acgatttttg tcacaccttc
    49381 ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg aaacttttag
    49441 cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt tttactggat
    49501 ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc atcgaaactg
    49561 attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa gaggccttag
    49621 atgtgatgca agatagtttt cataaagcct tgaactattt tgaaaaagaa aaagagatcg
    49681 agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat agttggcgca
    49741 gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa cagttacctt
    49801 tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt ccggaaaaag
    49861 agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgca actatcttgg
    49921 atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa
    49981 tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt aaaaaaacta
    50041 aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat ccgccagcgt
    50101 tttattatcg aagagcaatc atttataaaa gcaaggcgga gaaagagacg tctccaaggg
    50161 ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt taatagtcaa
    50221 gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt gatcttaggc
    50281 gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat caatacacaa
    50341 aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg cgaaggacaa
    50401 gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca ttaccaagtt
    50461 ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga aaggcagatc
    50521 acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga caagaaaaac
    50581 agtgtccagc tctcacttcc acttttattc aaagatgaca cttatctatc tgttttgaca
    50641 aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa gtactattgg
    50701 actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac gcctacacga
    50761 agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata cgaaattgct
    50821 cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa aacgattttg
    50881 gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca gtttcaattt
    50941 tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat atagtgaaaa
    51001 gaaattattt agtcatcagt attattgctt tatccgtgct tttgtttttc gaaattactt
    51061 cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag cattcgtcac
    51121 aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa acagctaacg
    51181 tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcaatcatca caatcaacac
    51241 aagacagtac agaagcaatc caagacccga ctgtcaaaag cgtcgcaatc agctttgatg
    51301 atggaccagg agcgaccaca acgccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc
    51361 atgtcacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga
    51421 cagctgaagc agggcacgag attggcaatc acacatacga tcatcagaat ctggccactc
    51481 tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc aaaaaaagtc
    51541 actggcaaaa cccccgcttt tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca
    51601 acgatcatcc agcgaccgat catcgaatgg tctgttgatt cagaagattg gaagacaagg
    51661 aatcctgatt tgattttgca gaagatccaa gcaacagtat acgatgggtc aattatttta
    51721 ttccatgata tctatcctga aacgattcgt gccgttcctc aagtgatcga ttatctgaaa
    51781 gagcaaggct atcgcatcac gacagttagc gatttattag ggcatccaac ggctgtcgag
    51841 aactattatg gcagaaacga tcatcggcca gttcagtaag tgagacaagt atctttaata
    51901 aataacacat tttaataagt agttgaacac ataactacct tacttgcaaa ggcttaacaa
    51961 tatctataca gatacaacaa aaaactgtcc accaattctc tttggcggac agttttcact
    52021 taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga
    52081 gggaatcgaa cccccgtctc aagaaccgga atcttacgtg atatccacta cactaaggac
    52141 gcaagcactg ggataattgt aactaaacag ataaaaaatt acaaggggat tggacaaaaa
    52201 aatgaaattt gagcaaaatt tttcacaaaa acagcagcag acacagaaac tagccatgac
    52261 gcaacagttg caacagtcca tccaagtttt acaatataac agtgaggagc tgttggcatt
    52321 cgttgagaat caagcaatgg aaaatccatt ggtggaagtt gttgagccgg aatggcagcc
    52381 agattacata aaagcttctt catcttctta tgaaggagaa gagacaaatt acctaaatca
    52441 gattcctgat acaaaagggt ctttatttga ttcattgatc gaacaagtcc atttgaatta
    52501 ccgagatact tttttgagga aaatcgttct ttatcttgta gaatacatcg atttgaacgg
    52561 atttttgacg attagtttag aagaagccgg aaaagagatt ggagctacgc ctatccaaat
    52621 gctcgatgcg ttgactctga ttcagcaatt agaaccagct ggagtaggtg cacgtagttt
    52681 gcaagaatgt ctcatgcttc aaacagaacg agatgattat gcgccggaat tagcttatat
    52741 cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg
    52801 tttttctgtt gatctacatg ctgttcagca gatattcgac tatttacaaa cgttgagtcc
    52861 aagccctggc cggattttcg accgtagcag cgaattgtac attattcctg atgtccgggt
    52921 attagtggac gacgataaaa atgtacaagt tatctcaaac cgaaaaaatc agccaaatat
    52981 acgctttcaa gagagttact tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa
    53041 ttatttaaaa gaacggaaac aggaattcga atggttgaag aaaacgatcc tccaacgtgg
    53101 ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg caaaaagctt tctttatcga
    53161 caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca
    53221 tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg gaaacagatt tcggtgtatt
    53281 tgaattaaaa aaatttttca ctacacgaat tcctacgaat agtacagaac agacagaaga
    53341 cctttcagca gatacagcga agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa
    53401 caagccgttg tcggaccaga aattggttga attgctgaaa aaagatgaga tagcgatctc
    53461 aagacgaaca gtagcaaaat atcgtgattt attaggaatc ccgagttcat caaaaagaaa
    53521 acgttatgat aactaatcag tcaaaagact agaaaaagta tttcactttt tctagtcttt
    53581 ttagttgaaa aacgagagca aaactgttag acttacagtc gtgaggttga gatttccctt
    53641 tattgatgtg aaattcgaat agttaatttc tttttttatc gtatatgggt cgttttaagt
    53701 ctacattgga cgtaaagtgt ccaactaagg agatcgctat gctggatgaa ataaaaatga
    53761 ttgaatctgt tgcaccagat attatcgaag tgttgcaaga acgctataaa attttacgaa
    53821 atatctattg gatgcagccg attgggcgtc gaagtttatc ggaaagtatg gggatcacag
    53881 aacgagtctt acgaacagaa acagaccttt tgaaaaatct gaatttgatc gacacctcaa
    53941 aaagcggtat gacgctgaca actaagggcg aagaagttta tcaaagcctt gaaaacttca
    54001 tggatcagct tttaggtacg catcaaaccg aacaacagtt ggcagaatat tttggtaacc
    54061 aacgctgtat cgttgtttca ggaaacagcg aagagcagac caaagtggct gatgcattcg
    54121 gcgaagcgct gagcgaagcc ttagaccgac tacttccaga aggcgagaat atcatcgctg
    54181 tgatgggagg aactactatg gctgtggtag ccgaacaatt gtctaatctg gaaaataaga
    54241 aacggcataa cctgtttgtc cctgcaagag gcgggattgg tgaagcgata actgtacagg
    54301 caaattctgt cagtgcaaga atggctgcta aagcaaacgg taaccaccgt gcactttatg
    54361 ttccggaaca attaagtctg gcaacttaca attctttact caatgagcca tctatccaag
    54421 aagtcttaaa cttgattagt gaagcgaatt gtgttattca tagtattggg cgtgcattac
    54481 acatggcagc acgtcgtaag atgactgaaa aagaattagt catgctgaaa caagcaaatg
    54541 ctgttgcgga atcttttggc tactttttca atgaaaaagg tgaagtcgtt tataaggtcc
    54601 ctagaatcgg catagaattg caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg
    54661 gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa aaatgcacca aaacaaacgt
    54721 ggctcatcac tgatgaagct gctgcaaatg agattttaaa aggggtaacc ctttaaaata
    54781 aatatttttt tgattttcat aaggaggaaa tcttaaatga cagttaaagt aggtattaat
    54841 ggttttggac gtattggacg tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa
    54901 gtagtagcaa tcaacgactt gacagatgct aaaatgttgg cacacttgtt aaaatacgac
    54961 acaactcaag gacgtttcaa cggaacagtt gaagttcatg aaggttcatt caacgtaaac
    55021 ggaaaagaag ttaaagtatt agctaaccgc aacccagaag aattaccatg gggcgaacta
    55081 ggcgtagata tcgttctaga atgtactggt ttcttcactt ctaaatcagc agctgaaaaa
    55141 catttaacag ctggtgctaa acgcgttgtt atctctgctc ctggcggaaa cgatgttcca
    55201 acaatcgttt ataacacaaa ccacgaaaca ttaactggta aagaaacagt tatctcaggt
    55261 gcttcatgta ctactaactg tttagctcct atggctaaaa cattgaacga caaatttggt
    55321 gttgttgaag gattaatgac aactatccac gcttacacag gtgaccaaat gactctagac
    55381 ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct
    55441 aactcaacag gtgctgctaa agctatcggt ttagtaattc ctgaattgaa cggtaaatta
    55501 gacggagctg ctcaacgtgt tcctgtacca acaggttcat taacagaatt agtaacagtt
    55561 cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa
    55621 tcttacggat acaacacaga cgaaatcgtt tcttcagata tcgttggtat gacttacggt
    55681 tcattatttg atgctacaca aactaaagta atgacagttg gcgacaaaca attagttaaa
    55741 actgttgctt ggtacgacaa cgaaatgtca tacactgcac aattagtacg tactttagaa
    55801 tacttcgcta acttataaga tagacaaacg tacaagtgta aattatagac atgtacattt
    55861 aaaagcgggg aagcaatcgc gcttcttccg ctttttttga taataagttt attcaggagg
    55921 aaataaaatg gctaagaaaa cagtgaaaga tatcgattta aaagacaaaa aagttcttgt
    55981 ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa
    56041 agcagcattg ccaacaatca actacgttct agaacaaggc ggaaaagcaa tccttttctc
    56101 tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga aaatctttag ctccagttgc
    56161 aaaacgtcta ggcgaattat taggtaaaga agtaacattc gtacctgaaa ctcgcggaga
    56221 acaattagaa gaagcaatcc gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac
    56281 acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat gatacagaac taggtaaata
    56341 ctgggcttca ttaggcgatg tatttgttaa cgatgctttc ggtacagctc accgtgcaca
    56401 tgcttctaac gtaggtatcg cttcaactgg tatcccaaca gttgctggat tcttgatgga
    56461 aaaagaaatc aagttcatcg gtgaagcagt agaagaacca aaacgaccaa tgattgcaat
    56521 ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc
    56581 agataaaatc ttgatcggtg gcggaatgac ttacacattc tacgaagcaa aaggaatcaa
    56641 gatcggtaac tctctagtag aagctgataa agtggaacta gcaaaagaat taatcgaaaa
    56701 agcaggcgac aaacttgtct tgccaatcga caatgtctgt gcaccagaat tttcaaatga
    56761 tgttgaaaca caagtaatcg aaggagacat tcctgatggt ttgatggctt tagacatcgg
    56821 cccagcatca gtaaaattat ttgctgatac attacaaggt gcaaaaacag ttgtttggaa
    56881 cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga
    56941 agcaatcgct aacttagaag gcgcaacaac aatcatcggt ggcggagact cagccgcagc
    57001 agcagaacaa ttaggatttg ctgacaaatt cacacacatc tcaacaggtg gcggcgcaag
    57061 cttggaattg cttgaaggta aagaattacc aggactagca gcaatcaacg ataaataaac
    57121 gaccaagttt gctcaattct gacgagtaat cgatcaaaag gtgaagggag ttttcatgac
    57181 tccctagcca tttttcaata aattaaggag tgtgttcctc aatgcgtaaa cctattatcg
    57241 caggtaactg gaaaatgaac aaaactgcat cagaagcaaa agaatttgct gaagcagtaa
    57301 aaaacaaaat cccttcaaat gacaaagttg attcagtcat cggatctcct gcattattct
    57361 tacaagaatt aatgacatca gctgaaggta cagaactaaa aatctcagca caaaactgct
    57421 actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc agcagctctt gctgatctag
    57481 gtgttcatta tgtgatcatc ggacattcag aacgtcgtga atatttccat gaaacagacg
    57541 aagacatcaa taaaaaagcg aaagctatct ttgcaaatgg tatgacacca atcctatgct
    57601 gtggcgaatc tttagaaacc tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa
    57661 ttacagctgg tttagctgat ctttcagctg aacaagtgtc aaacatggtt attgcttatg
    57721 aaccaatctg ggcaatcggt actggtaaat cagctgacgc acaaatcgct gacgaaattt
    57781 gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa agaagtatca gaagcagttc
    57841 gtatccaata cggcggttct gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa
    57901 atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc agattcattc ttgtcattgt
    57961 tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat
    58021 gagcaaaatt aactaaaatc gtatatgagg gaaatccctt aggaaaaaac aaactcaaag
    58081 gagagacaaa acatgtcaat tattactgat gtttacgctc gcgagatctt agactcacgt
    58141 ggtaacccaa caatcgaagt agaagtatac acagaaagcg gagcttttgg ccgcggaatg
    58201 gttccttcag gtgcttcaac tggtgaatac gaagcagttg aattacgtga cggagacaaa
    58261 gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca acgtaaacaa catcattgct
    58321 gaagcaatca ttggctacga tgttcgtgac caaatggcta tcgacaaagc tatgatcgct
    58381 ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg ctattttagg tgtttctatc
    58441 gctgttgcac gtgcagctgc tgactaccta gaagtacctt tataccacta cctaggcgga
    58501 ttcaacacaa aagtattgcc aacaccaatg atgaacatca tcaacggcgg atcacatgct
    58561 gataactcaa tcgacttcca agaattcatg atcatgcctg ttggcgctcc aacattcaaa
    58621 gaagctctac gtatgggtgc tgaagtattc cacgcattag cttcaatctt gaaagctcgc
    58681 ggattagcaa cttctgttgg tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa
    58741 gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac
    58801 gttgttcttg ctatggatgc tgcttcttca gaattctacg acaaagaaaa aggtgtttac
    58861 gtactagctg attcaggcga aggcgaaaaa acaactgacg aaatgatcaa attctacgaa
    58921 gaattagttt ctaaatatcc aatcatctca atcgaagacg gattagacga aaacgactgg
    58981 gacggattca agaaattaac tgacgtatta ggcgacaaag ttcaattagt tggtgacgac
    59041 ttgttcgtaa caaacactca aaaattatct gaaggtatcg aaaaaggaat tgctaactca
    59101 atccttatca aagtgaacca aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg
    59161 gctaaagaag ctggctacac agcagttgta tctcaccgtt ctggtgaaac agaagattca
    59221 acaatctctg atatcgctgt tgcaacaaac gctggccaaa tcaaaactgg ttctctatca
    59281 cgtactgacc gtattgctaa atacaaccaa ttactacgta tcgaagacca acttggtgaa
    59341 gttgcagaat acaaaggttt gaaatctttc tacaacttaa aaaacaaata attcttttct
    59401 aatgaagata catgatcgac tattgaaaga tagtcgagta tgatcactta gttaagtaat
    59461 cgaactctct acttgctgaa ata
//
LOCUS       DACDBG010000009        57756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDBG010000009 DACDBG010000000
VERSION     DACDBG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.009"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     regulatory      <1..69
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            185..766
                     /locus_tag="IVZ90_000532"
     CDS             185..766
                     /locus_tag="IVZ90_000532"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8261603.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            769..2076
                     /locus_tag="IVZ90_000533"
     CDS             769..2076
                     /locus_tag="IVZ90_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8261604.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2369..2857
                     /gene="purE"
                     /locus_tag="IVZ90_000534"
     CDS             2369..2857
                     /gene="purE"
                     /locus_tag="IVZ90_000534"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8261605.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2841..3974
                     /gene="purK"
                     /locus_tag="IVZ90_000535"
     CDS             2841..3974
                     /gene="purK"
                     /locus_tag="IVZ90_000535"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8261606.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            3987..5282
                     /gene="purB"
                     /locus_tag="IVZ90_000536"
     CDS             3987..5282
                     /gene="purB"
                     /locus_tag="IVZ90_000536"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8261607.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5462..5839)
                     /locus_tag="IVZ90_000537"
     CDS             complement(5462..5839)
                     /locus_tag="IVZ90_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261608.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6095..6823
                     /locus_tag="IVZ90_000538"
     CDS             6095..6823
                     /locus_tag="IVZ90_000538"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8261609.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6823..7077
                     /gene="purS"
                     /locus_tag="IVZ90_000539"
     CDS             6823..7077
                     /gene="purS"
                     /locus_tag="IVZ90_000539"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8261610.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7079..7750
                     /gene="purQ"
                     /locus_tag="IVZ90_000540"
     CDS             7079..7750
                     /gene="purQ"
                     /locus_tag="IVZ90_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8261611.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7751..9973
                     /gene="purL"
                     /locus_tag="IVZ90_000541"
     CDS             7751..9973
                     /gene="purL"
                     /locus_tag="IVZ90_000541"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8261612.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            9958..11397
                     /locus_tag="IVZ90_000542"
     CDS             9958..11397
                     /locus_tag="IVZ90_000542"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8261613.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11429..12472
                     /locus_tag="IVZ90_000543"
     CDS             11429..12472
                     /locus_tag="IVZ90_000543"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8261614.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12469..13047
                     /gene="purN"
                     /locus_tag="IVZ90_000544"
     CDS             12469..13047
                     /gene="purN"
                     /locus_tag="IVZ90_000544"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8261615.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13044..14579
                     /gene="purH"
                     /locus_tag="IVZ90_000545"
     CDS             13044..14579
                     /gene="purH"
                     /locus_tag="IVZ90_000545"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8261616.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14595..15869
                     /gene="purD"
                     /locus_tag="IVZ90_000546"
     CDS             14595..15869
                     /gene="purD"
                     /locus_tag="IVZ90_000546"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8261617.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            15991..16905
                     /locus_tag="IVZ90_000547"
     CDS             15991..16905
                     /locus_tag="IVZ90_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8261618.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17038..18603)
                     /locus_tag="IVZ90_000548"
     CDS             complement(17038..18603)
                     /locus_tag="IVZ90_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8261619.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18596..20047)
                     /locus_tag="IVZ90_000549"
     CDS             complement(18596..20047)
                     /locus_tag="IVZ90_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8261620.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20061..21077)
                     /locus_tag="IVZ90_000550"
     CDS             complement(20061..21077)
                     /locus_tag="IVZ90_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8261621.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21297..22385
                     /locus_tag="IVZ90_000551"
     CDS             21297..22385
                     /locus_tag="IVZ90_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8261622.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22439..23965
                     /locus_tag="IVZ90_000552"
     CDS             22439..23965
                     /locus_tag="IVZ90_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8261623.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            23967..25172
                     /locus_tag="IVZ90_000553"
     CDS             23967..25172
                     /locus_tag="IVZ90_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8261624.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25429..26406
                     /gene="guaC"
                     /locus_tag="IVZ90_000554"
     CDS             25429..26406
                     /gene="guaC"
                     /locus_tag="IVZ90_000554"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8261625.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26670..28094
                     /locus_tag="IVZ90_000555"
     CDS             26670..28094
                     /locus_tag="IVZ90_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8261626.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28555..29664
                     /gene="pdhA"
                     /locus_tag="IVZ90_000556"
     CDS             28555..29664
                     /gene="pdhA"
                     /locus_tag="IVZ90_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8261627.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29667..30644
                     /locus_tag="IVZ90_000557"
     CDS             29667..30644
                     /locus_tag="IVZ90_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8261628.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            30669..32312
                     /locus_tag="IVZ90_000558"
     CDS             30669..32312
                     /locus_tag="IVZ90_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8261629.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            32320..33726
                     /gene="lpdA"
                     /locus_tag="IVZ90_000559"
     CDS             32320..33726
                     /gene="lpdA"
                     /locus_tag="IVZ90_000559"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8261630.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            33888..34163
                     /locus_tag="IVZ90_000560"
     CDS             33888..34163
                     /locus_tag="IVZ90_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8261631.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            34169..34936
                     /locus_tag="IVZ90_000561"
     CDS             34169..34936
                     /locus_tag="IVZ90_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8261632.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            35104..36933
                     /gene="typA"
                     /locus_tag="IVZ90_000562"
     CDS             35104..36933
                     /gene="typA"
                     /locus_tag="IVZ90_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8261633.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            37194..38966
                     /gene="recQ"
                     /locus_tag="IVZ90_000563"
     CDS             37194..38966
                     /gene="recQ"
                     /locus_tag="IVZ90_000563"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8261634.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            39080..39382
                     /locus_tag="IVZ90_000564"
     CDS             39080..39382
                     /locus_tag="IVZ90_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8261635.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            39385..40548
                     /locus_tag="IVZ90_000565"
     CDS             39385..40548
                     /locus_tag="IVZ90_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8261636.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            40574..44002
                     /locus_tag="IVZ90_000566"
     CDS             40574..44002
                     /locus_tag="IVZ90_000566"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8261637.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            44023..45147
                     /locus_tag="IVZ90_000567"
     CDS             44023..45147
                     /locus_tag="IVZ90_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261638.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            45137..45574
                     /locus_tag="IVZ90_000568"
     CDS             45137..45574
                     /locus_tag="IVZ90_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8261639.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            45558..45896
                     /locus_tag="IVZ90_000569"
     CDS             45558..45896
                     /locus_tag="IVZ90_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8261640.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            46015..46575
                     /gene="rsmD"
                     /locus_tag="IVZ90_000570"
     CDS             46015..46575
                     /gene="rsmD"
                     /locus_tag="IVZ90_000570"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8261641.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            46572..47063
                     /gene="coaD"
                     /locus_tag="IVZ90_000571"
     CDS             46572..47063
                     /gene="coaD"
                     /locus_tag="IVZ90_000571"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8261642.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            47053..48096
                     /locus_tag="IVZ90_000572"
     CDS             47053..48096
                     /locus_tag="IVZ90_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8261643.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            48255..48983
                     /locus_tag="IVZ90_000573"
     CDS             48255..48983
                     /locus_tag="IVZ90_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8261644.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            49099..49344
                     /locus_tag="IVZ90_000574"
     CDS             49099..49344
                     /locus_tag="IVZ90_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8261645.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            49341..49559
                     /locus_tag="IVZ90_000575"
     CDS             49341..49559
                     /locus_tag="IVZ90_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8261646.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            49695..50234
                     /locus_tag="IVZ90_000576"
     CDS             49695..50234
                     /locus_tag="IVZ90_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8261647.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            50227..52500
                     /locus_tag="IVZ90_000577"
     CDS             50227..52500
                     /locus_tag="IVZ90_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8261648.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            52538..53584
                     /gene="holA"
                     /locus_tag="IVZ90_000578"
     CDS             52538..53584
                     /gene="holA"
                     /locus_tag="IVZ90_000578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8261649.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(53673..53927)
                     /locus_tag="IVZ90_000579"
     CDS             complement(53673..53927)
                     /locus_tag="IVZ90_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8261650.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(54327..54596)
                     /locus_tag="IVZ90_000580"
     CDS             complement(54327..54596)
                     /locus_tag="IVZ90_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261651.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(55066..55521)
                     /locus_tag="IVZ90_000581"
     CDS             complement(55066..55521)
                     /locus_tag="IVZ90_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8261652.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(55659..57587)
                     /locus_tag="IVZ90_000582"
     CDS             complement(55659..57587)
                     /locus_tag="IVZ90_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8261653.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        18885 a         9922 c        12676 g        16273 t
ORIGIN      
        1 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
       61 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
      121 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
      181 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
      241 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
      301 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
      361 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
      421 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
      481 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
      541 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
      601 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
      661 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
      721 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
      781 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
      841 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
      901 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
      961 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
     1021 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
     1081 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
     1141 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
     1201 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
     1261 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
     1321 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
     1381 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
     1441 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
     1501 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
     1561 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
     1621 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
     1681 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
     1741 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
     1801 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
     1861 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
     1921 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
     1981 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
     2041 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
     2101 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
     2161 gtttgtttga gctaataccg agggtcagct gattgaacac cgtttatcca taaaaaggaa
     2221 tgtgacctaa cttttgtcac attccttttt ttcatcttta ataaacacga acaataaaat
     2281 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
     2341 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
     2401 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
     2461 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
     2521 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
     2581 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
     2641 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
     2701 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
     2761 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
     2821 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
     2881 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
     2941 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
     3001 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
     3061 atgtcattac ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg attttaccca
     3121 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
     3181 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
     3241 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
     3301 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
     3361 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
     3421 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
     3481 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
     3541 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
     3601 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
     3661 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
     3721 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
     3781 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
     3841 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
     3901 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
     3961 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
     4021 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
     4081 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
     4141 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
     4201 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
     4261 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
     4321 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
     4381 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
     4441 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
     4501 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
     4561 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atctctactc
     4621 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
     4681 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
     4741 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
     4801 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
     4861 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
     4921 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
     4981 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
     5041 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
     5101 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
     5161 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
     5221 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
     5281 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
     5341 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
     5401 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
     5461 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
     5521 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
     5581 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
     5641 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
     5701 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
     5761 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
     5821 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
     5881 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
     5941 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
     6001 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
     6061 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
     6121 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
     6181 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
     6241 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
     6301 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     6361 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     6421 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     6481 tccatttatc aatgaagagc atatctattt cttacaactt gcaaaaccgg aagaaatcag
     6541 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     6601 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     6661 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     6721 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     6781 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     6841 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     6901 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     6961 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     7021 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     7081 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     7141 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     7201 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     7261 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     7321 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     7381 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     7441 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     7501 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     7561 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     7621 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     7681 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     7741 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     7801 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     7861 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     7921 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     7981 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     8041 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     8101 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     8161 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     8221 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     8281 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     8341 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     8401 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     8461 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     8521 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     8581 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     8641 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     8701 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     8761 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     8821 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     8881 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     8941 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     9001 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     9061 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     9121 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     9181 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     9241 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     9301 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     9361 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     9421 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     9481 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     9541 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     9601 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     9661 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     9721 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     9781 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     9841 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     9901 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     9961 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    10021 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    10081 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    10141 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    10201 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    10261 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    10321 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    10381 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    10441 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    10501 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    10561 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    10621 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    10681 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    10741 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    10801 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    10861 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    10921 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    10981 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    11041 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    11101 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    11161 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    11221 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    11281 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    11341 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    11401 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    11461 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    11521 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    11581 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    11641 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    11701 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    11761 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    11821 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    11881 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    11941 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    12001 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    12061 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    12121 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    12181 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    12241 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    12301 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    12361 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    12421 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    12481 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    12541 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    12601 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    12661 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    12721 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    12781 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    12841 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    12901 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    12961 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    13021 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    13081 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    13141 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    13201 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    13261 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    13321 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    13381 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    13441 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    13501 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    13561 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    13621 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    13681 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    13741 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    13801 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    13861 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    13921 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    13981 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    14041 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    14101 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    14161 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    14221 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
    14281 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    14341 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    14401 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    14461 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    14521 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    14581 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    14641 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    14701 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    14761 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    14821 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    14881 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    14941 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    15001 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    15061 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    15121 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    15181 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    15241 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    15301 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    15361 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    15421 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    15481 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    15541 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    15601 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    15661 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    15721 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    15781 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    15841 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    15901 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    15961 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    16021 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    16081 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    16141 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    16201 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    16261 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    16321 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    16381 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    16441 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    16501 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    16561 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    16621 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    16681 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    16741 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    16801 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    16861 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    16921 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    16981 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    17041 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    17101 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    17161 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    17221 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    17281 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    17341 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    17401 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    17461 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    17521 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    17581 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    17641 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    17701 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    17761 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    17821 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    17881 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    17941 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    18001 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    18061 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    18121 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    18181 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    18241 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    18301 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    18361 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    18421 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    18481 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    18541 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    18601 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    18661 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    18721 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    18781 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    18841 ctttgaattg gatcatctca tcaattttgc atgcggtaat acgaataaat cctttgtgac
    18901 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    18961 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    19021 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    19081 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    19141 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    19201 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    19261 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    19321 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    19381 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    19441 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    19501 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    19561 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    19621 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    19681 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    19741 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    19801 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    19861 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    19921 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    19981 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    20041 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    20101 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    20161 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    20221 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    20281 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    20341 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    20401 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    20461 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    20521 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    20581 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    20641 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    20701 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    20761 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    20821 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    20881 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    20941 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    21001 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    21061 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    21121 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    21181 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    21241 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    21301 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    21361 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    21421 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    21481 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    21541 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    21601 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    21661 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    21721 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    21781 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    21841 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    21901 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    21961 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    22021 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    22081 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    22141 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    22201 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    22261 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    22321 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    22381 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    22441 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    22501 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    22561 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    22621 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    22681 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    22741 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    22801 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    22861 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    22921 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    22981 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    23041 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    23101 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg ggatggtggg
    23161 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    23221 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    23281 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    23341 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    23401 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    23461 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    23521 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    23581 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    23641 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    23701 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    23761 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    23821 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    23881 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    23941 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    24001 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    24061 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    24121 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    24181 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    24241 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    24301 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    24361 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    24421 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    24481 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    24541 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    24601 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    24661 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    24721 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    24781 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    24841 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    24901 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    24961 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    25021 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    25081 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    25141 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    25201 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    25261 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    25321 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    25381 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    25441 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    25501 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    25561 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    25621 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    25681 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    25741 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    25801 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    25861 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    25921 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    25981 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    26041 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    26101 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    26161 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    26221 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    26281 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    26341 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    26401 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    26461 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    26521 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    26581 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    26641 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    26701 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    26761 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    26821 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    26881 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    26941 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    27001 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    27061 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    27121 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    27181 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    27241 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    27301 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    27361 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    27421 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    27481 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    27541 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    27601 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    27661 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    27721 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    27781 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    27841 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    27901 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    27961 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    28021 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    28081 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    28141 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    28201 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    28261 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    28321 atgcagatga aaaataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    28381 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    28441 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    28501 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    28561 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    28621 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    28681 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    28741 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    28801 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    28861 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    28921 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    28981 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    29041 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    29101 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    29161 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    29221 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    29281 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    29341 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    29401 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    29461 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    29521 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    29581 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    29641 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    29701 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    29761 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    29821 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    29881 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    29941 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    30001 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    30061 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    30121 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    30181 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    30241 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    30301 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    30361 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    30421 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    30481 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    30541 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    30601 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    30661 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    30721 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    30781 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    30841 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    30901 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    30961 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    31021 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    31081 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    31141 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    31201 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    31261 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    31321 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    31381 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    31441 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    31501 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    31561 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    31621 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    31681 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    31741 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    31801 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    31861 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    31921 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    31981 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    32041 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    32101 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    32161 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    32221 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    32281 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    32341 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    32401 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    32461 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    32521 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    32581 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    32641 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    32701 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    32761 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    32821 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    32881 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    32941 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    33001 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    33061 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    33121 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    33181 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    33241 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    33301 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    33361 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    33421 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    33481 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    33541 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    33601 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    33661 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    33721 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    33781 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    33841 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    33901 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    33961 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    34021 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    34081 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    34141 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    34201 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    34261 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    34321 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    34381 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    34441 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    34501 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    34561 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    34621 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    34681 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    34741 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    34801 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    34861 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    34921 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    34981 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    35041 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    35101 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    35161 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    35221 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    35281 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    35341 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    35401 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    35461 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    35521 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    35581 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    35641 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    35701 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    35761 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    35821 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    35881 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    35941 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    36001 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    36061 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    36121 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    36181 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    36241 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    36301 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    36361 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    36421 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    36481 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    36541 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    36601 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    36661 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    36721 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    36781 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    36841 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    36901 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    36961 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    37021 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    37081 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    37141 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    37201 acttattaaa acaatacttt ggttatgatg agtttagacc tggtcaaaaa gaaattatcc
    37261 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    37321 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    37381 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    37441 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    37501 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    37561 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    37621 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    37681 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    37741 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    37801 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    37861 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    37921 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    37981 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    38041 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    38101 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    38161 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    38221 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    38281 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    38341 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    38401 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    38461 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    38521 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    38581 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    38641 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    38701 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    38761 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    38821 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    38881 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    38941 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    39001 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    39061 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    39121 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    39181 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    39241 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    39301 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    39361 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    39421 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    39481 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    39541 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    39601 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    39661 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    39721 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    39781 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    39841 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    39901 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    39961 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    40021 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    40081 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    40141 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    40201 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    40261 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    40321 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    40381 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    40441 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    40501 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    40561 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    40621 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    40681 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    40741 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    40801 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    40861 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    40921 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    40981 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    41041 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    41101 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    41161 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    41221 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    41281 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    41341 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    41401 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    41461 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    41521 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    41581 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    41641 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    41701 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    41761 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    41821 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    41881 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    41941 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    42001 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    42061 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    42121 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    42181 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    42241 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    42301 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    42361 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    42421 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    42481 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    42541 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    42601 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    42661 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    42721 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    42781 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    42841 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    42901 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    42961 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    43021 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    43081 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    43141 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    43201 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    43261 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    43321 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    43381 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    43441 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    43501 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    43561 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    43621 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    43681 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    43741 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    43801 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    43861 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    43921 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    43981 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    44041 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    44101 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    44161 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    44221 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    44281 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    44341 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    44401 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    44461 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    44521 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    44581 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    44641 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    44701 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    44761 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    44821 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    44881 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    44941 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    45001 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    45061 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    45121 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    45181 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    45241 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    45301 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    45361 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    45421 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    45481 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    45541 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    45601 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    45661 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    45721 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    45781 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    45841 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    45901 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    45961 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    46021 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    46081 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    46141 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    46201 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    46261 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    46321 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    46381 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    46441 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    46501 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    46561 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    46621 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    46681 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    46741 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    46801 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    46861 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    46921 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    46981 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    47041 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    47101 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    47161 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    47221 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    47281 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    47341 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    47401 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    47461 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    47521 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    47581 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    47641 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    47701 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    47761 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    47821 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    47881 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    47941 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    48001 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    48061 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    48121 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    48181 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    48241 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    48301 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    48361 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    48421 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    48481 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    48541 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    48601 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    48661 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    48721 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    48781 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    48841 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    48901 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    48961 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    49021 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    49081 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    49141 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    49201 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    49261 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    49321 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    49381 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    49441 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    49501 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    49561 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    49621 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    49681 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    49741 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    49801 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    49861 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    49921 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    49981 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    50041 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    50101 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    50161 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    50221 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    50281 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    50341 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    50401 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    50461 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    50521 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    50581 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    50641 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    50701 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    50761 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    50821 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    50881 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    50941 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttatttcga
    51001 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    51061 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    51121 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    51181 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    51241 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    51301 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    51361 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    51421 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    51481 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    51541 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    51601 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    51661 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    51721 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    51781 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    51841 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    51901 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    51961 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    52021 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    52081 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    52141 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    52201 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    52261 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    52321 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    52381 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    52441 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    52501 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    52561 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    52621 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    52681 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    52741 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    52801 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    52861 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    52921 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    52981 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    53041 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    53101 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    53161 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    53221 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    53281 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    53341 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    53401 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    53461 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    53521 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    53581 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    53641 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    53701 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    53761 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    53821 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    53881 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    53941 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    54001 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    54061 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    54121 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    54181 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    54241 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    54301 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    54361 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    54421 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    54481 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    54541 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    54601 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    54661 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    54721 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    54781 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    54841 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    54901 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    54961 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    55021 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    55081 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    55141 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    55201 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    55261 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    55321 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    55381 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    55441 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    55501 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    55561 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    55621 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    55681 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    55741 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    55801 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    55861 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    55921 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    55981 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    56041 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    56101 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    56161 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    56221 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    56281 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    56341 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    56401 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    56461 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    56521 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    56581 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    56641 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    56701 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    56761 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    56821 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    56881 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    56941 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    57001 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    57061 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    57121 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    57181 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    57241 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    57301 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    57361 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    57421 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    57481 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    57541 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    57601 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    57661 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    57721 ttattattaa agaaaataaa aaaccagaac atttct
//
LOCUS       DACDBG010000010        52888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDBG010000010 DACDBG010000000
VERSION     DACDBG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.010"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..1183
                     /locus_tag="IVZ90_000583"
     CDS             122..1183
                     /locus_tag="IVZ90_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8261654.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1186..2082
                     /gene="murQ"
                     /locus_tag="IVZ90_000584"
     CDS             1186..2082
                     /gene="murQ"
                     /locus_tag="IVZ90_000584"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8261655.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2096..4078
                     /locus_tag="IVZ90_000585"
     CDS             2096..4078
                     /locus_tag="IVZ90_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261656.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4143..4589)
                     /gene="nrdI"
                     /locus_tag="IVZ90_000586"
     CDS             complement(4143..4589)
                     /gene="nrdI"
                     /locus_tag="IVZ90_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8261657.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4800..6224
                     /locus_tag="IVZ90_000587"
     CDS             4800..6224
                     /locus_tag="IVZ90_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261658.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6405..7178)
                     /locus_tag="IVZ90_000588"
     CDS             complement(6405..7178)
                     /locus_tag="IVZ90_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8261659.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7175..8080)
                     /locus_tag="IVZ90_000589"
     CDS             complement(7175..8080)
                     /locus_tag="IVZ90_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261660.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8234..8554)
                     /locus_tag="IVZ90_000590"
     CDS             complement(8234..8554)
                     /locus_tag="IVZ90_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8261661.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            9028..9714
                     /locus_tag="IVZ90_000591"
     CDS             9028..9714
                     /locus_tag="IVZ90_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8261662.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            9716..10660
                     /locus_tag="IVZ90_000592"
     CDS             9716..10660
                     /locus_tag="IVZ90_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8261663.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            10859..11503
                     /gene="pcp"
                     /locus_tag="IVZ90_000593"
     CDS             10859..11503
                     /gene="pcp"
                     /locus_tag="IVZ90_000593"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8261664.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            11582..12859
                     /locus_tag="IVZ90_000594"
     CDS             11582..12859
                     /locus_tag="IVZ90_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261665.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13043..13792)
                     /locus_tag="IVZ90_000595"
     CDS             complement(13043..13792)
                     /locus_tag="IVZ90_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8261666.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            14122..14502
                     /locus_tag="IVZ90_000596"
     CDS             14122..14502
                     /locus_tag="IVZ90_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8261667.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            14598..15107
                     /locus_tag="IVZ90_000597"
     CDS             14598..15107
                     /locus_tag="IVZ90_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261668.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            15184..15363
                     /locus_tag="IVZ90_000598"
     CDS             15184..15363
                     /locus_tag="IVZ90_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261669.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            15418..15981
                     /locus_tag="IVZ90_000599"
     CDS             15418..15981
                     /locus_tag="IVZ90_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261670.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(16028..16543)
                     /locus_tag="IVZ90_000600"
     CDS             complement(16028..16543)
                     /locus_tag="IVZ90_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261671.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(16569..17438)
                     /locus_tag="IVZ90_000601"
     CDS             complement(16569..17438)
                     /locus_tag="IVZ90_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8261672.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            17564..18244
                     /locus_tag="IVZ90_000602"
     CDS             17564..18244
                     /locus_tag="IVZ90_000602"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8261673.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            18397..19377
                     /gene="pta"
                     /locus_tag="IVZ90_000603"
     CDS             18397..19377
                     /gene="pta"
                     /locus_tag="IVZ90_000603"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8261674.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            19500..19973
                     /gene="tsaE"
                     /locus_tag="IVZ90_000604"
     CDS             19500..19973
                     /gene="tsaE"
                     /locus_tag="IVZ90_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8261675.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            19966..20490
                     /locus_tag="IVZ90_000605"
     CDS             19966..20490
                     /locus_tag="IVZ90_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261676.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(20980..21513)
                     /locus_tag="IVZ90_000606"
     CDS             complement(20980..21513)
                     /locus_tag="IVZ90_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8261677.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(22499..23251)
                     /gene="xth"
                     /locus_tag="IVZ90_000607"
     CDS             complement(22499..23251)
                     /gene="xth"
                     /locus_tag="IVZ90_000607"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8261678.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            23364..24284
                     /locus_tag="IVZ90_000608"
     CDS             23364..24284
                     /locus_tag="IVZ90_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8261679.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            24357..25292
                     /gene="murB"
                     /locus_tag="IVZ90_000609"
     CDS             24357..25292
                     /gene="murB"
                     /locus_tag="IVZ90_000609"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8261680.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            25590..27068
                     /locus_tag="IVZ90_000610"
     CDS             25590..27068
                     /locus_tag="IVZ90_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8261681.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            27345..29405
                     /locus_tag="IVZ90_000611"
     CDS             27345..29405
                     /locus_tag="IVZ90_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261682.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            29488..30057
                     /locus_tag="IVZ90_000612"
     CDS             29488..30057
                     /locus_tag="IVZ90_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261683.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30232..31038)
                     /locus_tag="IVZ90_000613"
     CDS             complement(30232..31038)
                     /locus_tag="IVZ90_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261684.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            31176..32690
                     /locus_tag="IVZ90_000614"
     CDS             31176..32690
                     /locus_tag="IVZ90_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8261685.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(33093..34076)
                     /locus_tag="IVZ90_000615"
     CDS             complement(33093..34076)
                     /locus_tag="IVZ90_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8261686.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(34081..34728)
                     /locus_tag="IVZ90_000616"
     CDS             complement(34081..34728)
                     /locus_tag="IVZ90_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8261687.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(34813..35445)
                     /locus_tag="IVZ90_000617"
     CDS             complement(34813..35445)
                     /locus_tag="IVZ90_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8261688.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(35461..36012)
                     /gene="coaC"
                     /locus_tag="IVZ90_000618"
     CDS             complement(35461..36012)
                     /gene="coaC"
                     /locus_tag="IVZ90_000618"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8261689.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(36009..36746)
                     /locus_tag="IVZ90_000619"
     CDS             complement(36009..36746)
                     /locus_tag="IVZ90_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8261690.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(36743..37732)
                     /locus_tag="IVZ90_000620"
     CDS             complement(36743..37732)
                     /locus_tag="IVZ90_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8261691.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            37990..38532
                     /locus_tag="IVZ90_000621"
     CDS             37990..38532
                     /locus_tag="IVZ90_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8261692.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            38549..39634
                     /locus_tag="IVZ90_000622"
     CDS             38549..39634
                     /locus_tag="IVZ90_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261693.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            39646..40452
                     /locus_tag="IVZ90_000623"
     CDS             39646..40452
                     /locus_tag="IVZ90_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261694.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            40453..41280
                     /locus_tag="IVZ90_000624"
     CDS             40453..41280
                     /locus_tag="IVZ90_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261695.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            41277..42350
                     /locus_tag="IVZ90_000625"
     CDS             41277..42350
                     /locus_tag="IVZ90_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8261696.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            42778..45474
                     /locus_tag="IVZ90_000626"
     CDS             42778..45474
                     /locus_tag="IVZ90_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261697.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIAKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            45551..46039
                     /locus_tag="IVZ90_000627"
     CDS             45551..46039
                     /locus_tag="IVZ90_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8261698.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            46051..46545
                     /locus_tag="IVZ90_000628"
     CDS             46051..46545
                     /locus_tag="IVZ90_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8261699.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            46566..47375
                     /locus_tag="IVZ90_000629"
     CDS             46566..47375
                     /locus_tag="IVZ90_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8261700.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            47372..48193
                     /locus_tag="IVZ90_000630"
     CDS             47372..48193
                     /locus_tag="IVZ90_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8261701.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            48244..50343
                     /locus_tag="IVZ90_000631"
     CDS             48244..50343
                     /locus_tag="IVZ90_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8261702.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETMNYFAQLFGQLSMSDDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTF
                     GEQFLEGIGDEEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDPLYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNH
                     QAFVTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIYIQRRKR"
     gene            50340..51458
                     /locus_tag="IVZ90_000632"
     CDS             50340..51458
                     /locus_tag="IVZ90_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8261703.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            51497..52375
                     /locus_tag="IVZ90_000633"
     CDS             51497..52375
                     /locus_tag="IVZ90_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8261704.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            52566..52718
                     /locus_tag="IVZ90_000634"
     CDS             52566..52718
                     /locus_tag="IVZ90_000634"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261705.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        17020 a         9211 c        11036 g        15621 t
ORIGIN      
        1 atataaactc aacctttaga aatgaaattt aattgtttta ataaaagtat tgaaattttg
       61 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
      121 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
      181 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
      241 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
      301 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
      361 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
      421 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
      481 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
      541 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
      601 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
      661 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
      721 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
      781 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
      841 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
      901 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
      961 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     1021 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     1081 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     1141 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     1201 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     1261 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     1321 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     1381 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     1441 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     1501 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     1561 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     1621 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     1681 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     1741 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     1801 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     1861 ttgatggtcg atgtcaaacc aacaaacgaa aaactaatcg aacgctcaaa acgaatcatc
     1921 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
     1981 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
     2041 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
     2101 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
     2161 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
     2221 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
     2281 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
     2341 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
     2401 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
     2461 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
     2521 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
     2581 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
     2641 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
     2701 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
     2761 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
     2821 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
     2881 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
     2941 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
     3001 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
     3061 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
     3121 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
     3181 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
     3241 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
     3301 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
     3361 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
     3421 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
     3481 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
     3541 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
     3601 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
     3661 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
     3721 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
     3781 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
     3841 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
     3901 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
     3961 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
     4021 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
     4081 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
     4141 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
     4201 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
     4261 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
     4321 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
     4381 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
     4441 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
     4501 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
     4561 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
     4621 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
     4681 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
     4741 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
     4801 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
     4861 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
     4921 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
     4981 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
     5041 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
     5101 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
     5161 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
     5221 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
     5281 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
     5341 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
     5401 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
     5461 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
     5521 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
     5581 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
     5641 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
     5701 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
     5761 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
     5821 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
     5881 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
     5941 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
     6001 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
     6061 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
     6121 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
     6181 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
     6241 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
     6301 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
     6361 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
     6421 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
     6481 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
     6541 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
     6601 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
     6661 gcaccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
     6721 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
     6781 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
     6841 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
     6901 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
     6961 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
     7021 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
     7081 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
     7141 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
     7201 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
     7261 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
     7321 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
     7381 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
     7441 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
     7501 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
     7561 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
     7621 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
     7681 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
     7741 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
     7801 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
     7861 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
     7921 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
     7981 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
     8041 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
     8101 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
     8161 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
     8221 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
     8281 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
     8341 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
     8401 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
     8461 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
     8521 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
     8581 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
     8641 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
     8701 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
     8761 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
     8821 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
     8881 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
     8941 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
     9001 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
     9061 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
     9121 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
     9181 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
     9241 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
     9301 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
     9361 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
     9421 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
     9481 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
     9541 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
     9601 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
     9661 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
     9721 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
     9781 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
     9841 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
     9901 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
     9961 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    10021 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    10081 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    10141 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    10201 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    10261 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    10321 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    10381 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    10441 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    10501 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    10561 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    10621 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    10681 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    10741 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    10801 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    10861 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    10921 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    10981 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    11041 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    11101 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    11161 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    11221 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    11281 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    11341 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    11401 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    11461 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    11521 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    11581 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    11641 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    11701 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    11761 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    11821 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    11881 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    11941 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    12001 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    12061 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    12121 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    12181 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    12241 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    12301 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    12361 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    12421 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    12481 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    12541 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    12601 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    12661 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    12721 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    12781 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    12841 tctgaaggaa ttactataaa aaatagaggc tgggacagaa gcgtttaact ccaagaaata
    12901 agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta tttccgaagg
    12961 agttgcttct gattccgccg tttatacgtg tttagagcgt gagacaaaag tgttctttac
    13021 ttttgtccca cgctcagttt ttttatgcgt gttgttgtgc gttgtagtat tcttcttcca
    13081 ttttttgcat acgttcacgt tctttttggt tataccaagg tgaaacggta aatgcaagga
    13141 catcattcag caagtaaaca gagctgttca cgaacatcgc caaacttgca gatccatgcg
    13201 taaatgaaat ataccaaagg accatttgag caagccctga agctaaccac cagtaatatt
    13261 ggttgttgta acgcaagaaa ctgatgatac cagcagtcaa gctaatagaa aaagcaatgg
    13321 catctaccca cggccgagga tcatctgtat aacgaccaat caggaatcct gaaacaagat
    13381 agacaaggaa agtactgatg attgcgataa accagctttt tccagtgaat tttcggattt
    13441 ttgaagccat attatgattc cattctttac ttaacaatac ggggatatcc aatgtgatca
    13501 tataagcaat ctgttcaccg atacttaaat agttctttgc tgaaaaccca acgataataa
    13561 aacaaatcgc agacaaaata cctaaaatac cgttgactga ttttgccgca ttgatggata
    13621 aaatacacaa tacaccaagc gtggtaccga taaatgtaat aacggacagc caagtgatgg
    13681 gactattgac aagagtcatg acctggcagc caagactgaa aaagaaaaga taataatttt
    13741 gttgcggcca gccttttagc tgatgagcta aaaactgaaa atatgacttc atgtatgagg
    13801 actccttaag acactaaatg tagtgtgttc tgtatttact aacacaatct attgtatgct
    13861 tttcttgaaa aaaaactagt ctttcaatta taaataattt gtgttatgat aattttctgt
    13921 ttgttttctt gaaaatataa tttcttactt tcatttatta tattgggaac aggaagagtg
    13981 tcttggagga cctactttct gttttttttg ccttttttct tataaaaagg aaaaacagct
    14041 atttttttca atctgacaca tttacaatgg aactggagat taatatagat ccgagcttaa
    14101 acgaagaata ggagtgctca tatgaacctt acaacgatcc atcatatagc catcattgtt
    14161 tcagattatc agaaatcacg tgatttttat gttaataaat taggatttga agtaatcaga
    14221 gaaaattacc gcaaagaacg agatgactat aaattagatc tgaaactagg aagtgctgaa
    14281 ttggagattt ttggaattgc taatgcgcca caacgaccaa actatccaga agcttgtggc
    14341 ttgcgtcatc ttgctttcaa agtagacgat attgaagaaa caattgcaga attgcagtcc
    14401 ttagggatcg aatcagaacc gattagagaa gataccttta caggaaaaaa aatgacgttt
    14461 ttctttgatc cagacggatt gccattagaa ctgcacgaat aaagataaca aaattgtcac
    14521 ttttttttcg tttcattcga tttttcattc gttcatttct tgatattctt taaaaaaaag
    14581 aaacaggaga atgaagaatg aaaaaaatga tgtttggtgt tgctgccctc atcgtaatag
    14641 ggattaccgg tatgacggga ctgctcaatg ccgcttttca catggatgat attagtgacc
    14701 aggaatatcg gatctcattg aaaaaagcag cagcaatcgt aaaaaacgaa acaaaagaac
    14761 ataacatatc tgctattcat tttgcagttg aaaaagattt ggagaatcct acgaatgcaa
    14821 catatgaata ccagtttttt tgtgaaggac aagtcatagc agtcaatcca tccactggga
    14881 aaacaaccgt acgaaatgac acggaaaagg ccgcagaaca tctttttgat atagaaaaaa
    14941 cggaagagtt caaacaaccg caagcagcga tgaaggaagc agtcagctac tacgggaaaa
    15001 aagcaaaagt gaaagagtgg acacttctag tcaaagatga acgtccttat tataaagtcc
    15061 gattaataga tggagaacag gaccgtcagg tatggatatc tgcatagaag aaggatattg
    15121 cttctctttt ttacaatata agagtaagct aagagagtaa agatgaaagg aagttgagat
    15181 aacatggaaa aaatgaaatg ccctaattgc ggaaaaaaat ttgcatacga agaagtcaat
    15241 aatgtagtcg aacataatga taaagaaatg ccagtcgtct gcccttattg ccgaactgaa
    15301 gcggcaagaa tcgttactca tggttatttc attactgaaa agattgaaga ttttttaaaa
    15361 taacgatttt gcgatttctt tttctgcatg ttagaatgga gaaaaggaga ggaaactatg
    15421 attcgttttg cgagaaaaga agatgctcca gaaatcgccc cattgatctt agtgattcta
    15481 aaagatatgg aactgccatt tttgaagcag acaggggaac aaaagacttt agagatttta
    15541 gaagaagcaa tcactgatcc aaattatcgt tacagttatt cacgaggtat cgtagaagaa
    15601 atagacggta aagttgcagg agttgcttac ggttatacag atgaagaaga atcaatcatc
    15661 gatcagccac tggtacctat cttagagaaa tatcagctag atgcgtctac acgtttgttc
    15721 attgataaag aaacgtttcc caacgaatgg tatttggact ctatcagtgt atcagaagat
    15781 tttagaggac aaggcatcgg ttcgcgatta ttagaagcat tacctaaatt ggcgaagaaa
    15841 gctaatcgct ccgtcatcgg tctaagtgtg gatgaaaaaa atccaaaggc taaaaaatta
    15901 tacgaacgcc atgacttcaa agttgtcggt cagcggatga tcagtggtca tctttatgat
    15961 catatgcaaa agaatatcta aaaagatgta cctgaaatgc gtatttccgc atttcaggta
    16021 catcttttta acttaagtta agtcggtaag aaacaggctt atcgcgactt ttgtctttac
    16081 ggtagagcca gtaatctaaa accgcagcaa tacaaataca aactggagca tcttcatcat
    16141 ttgagacatc caatacatag taatctcctg aaaaaagtgt tgctttatcc attttcatga
    16201 tttgctgatt gaaatgatgg atttgatatt gatgattata gatatcacct tggactgtcc
    16261 agtgaagtct acgaatataa taaaagtcac cgggccaatt gaatattttc tgcatggttc
    16321 ccaccttttc gaactcttta tacagctcaa aacgcgtacc gaatgtcagg cttatctgct
    16381 tgatgtgagc aacgagatcg ccattcatgg cataaagaga caatgcatcg ccctttgttc
    16441 cccagcgacc aaccatcaaa aacagggaat gtcctttttc gtttttgaca attgtccgag
    16501 ttgcacgtcc aagctgcttt tcctgaatga aaaattctga catctatatc cctcttttct
    16561 agataaaact acaagtcttc tgcaatcgcg cgcaatatgg cttttcctac gccagattca
    16621 aggtttgtgt ccgttgtata tttggcataa tcttttactt cgatttctgc atttcccatt
    16681 gcgaaactga ctcctgctac ttgcagcatg ctgacatcat tgaagttatc gccaatcgtc
    16741 atgacttctt ctaaagaaat cccacgctcg tgagcgacat gggcaacagc aattcctttt
    16801 tgagcgtttt tatggttcac ttcgatattg ttttgaccag aggaagtaac ggctagatcg
    16861 cctaaaagag aaatctgctt tccaactgga cctaatacag ctggcccttc tgtatgaaaa
    16921 caaataattt ttaaaacttc cagattcttt tcttccaaaa tagcacgaat actgtccacg
    16981 tatgtgatgt gcaataactc cagcttagct gaggccatgg caatcgccat tttatacgta
    17041 aggtgaggta aatgggtagc caccatggaa gcaaaacttt caattcgttt cgcttgattt
    17101 tcagaataaa gaccatcgtt ggttgctact tcatagtaaa taccgttagc atctagaata
    17161 tctaaaacag agattgcttg attatttgga atcgacacgg taaacaatga atttccttct
    17221 ttatcaaata cctgtgcccc gttcaaagtg atcatcgcac aatcgattcc tacctcatct
    17281 aaagctgcgc gagcttcttg atagtttcga ccagtagcga ccatgaactc aacaccggca
    17341 tcatttgcaa aacgtatcgc ctcggcattt tcaggagaaa cactcatatg ggcatctaat
    17401 aatgtgccgt ccatatctga tgcaattaat ttgatcataa tgattatcgt tcctttctgc
    17461 ttcttgttcc tagtttacca tattaatcaa gtgctgaggg tgaagattgt cccttgtgat
    17521 tgaagaaaat gtgagttatt ctttttatta ggaggtggag tttttgaaag aaatcattca
    17581 taacagttgg caagaagttt tgtcctcaga attttcaaaa gattattatc tgcatctaag
    17641 agaattttta aaaaaagaat atgcgtccca aaagatccat ccagatatgt atcatatcta
    17701 tgaagcattg gaattgactc cttatgaaga agttaaagtg gtgattttag gacaagatcc
    17761 ttatcatgga gaaaatcaag ctcatggctt atctttttct gtgcaacctg gcgtaaagat
    17821 cccgccatcg ttaagaaata tctataaaga gctttatgat gatcttggaa tcgcacctgt
    17881 tcaacatgga aatctagtgt catgggctaa acagggagtc cttttactca acaccgttct
    17941 taccgttcgg gaagggcaag cgtattctca tcgcgggaaa ggttgggaaa gattaacaga
    18001 cacgatcatt gaaaaattaa atgaacggga aaaaccaatc gtgtttatct tgtggggaaa
    18061 accggcacaa gaaaaaatca aaatgatcga taagagtcgc catatcatca taacatctcc
    18121 acatcccagt ccgttatctg caagtcgtgg cttttttggt tccaaaccct tctcgaaaac
    18181 gaatgatgcg ctccttgcgt taggagaaga accgattgat tggcaactgc ctgaaacagt
    18241 gtaataaaac agcgctttcc tgttatagtg cagttcgctt tgtatgtgaa aaacttaatt
    18301 tttccagcat ttttgactga tacagataaa ataaactgtt ctttttttct ggaatagtgt
    18361 atgataatac atataaaaac gtctggaggt catcttgtgg aattgtttga tagtttaaga
    18421 tttaaaattg ttcgccgtgg gatcaaaatc gttttcccgg aagctactga tgctcgtatt
    18481 ttaggcgcag ctgcccggtt gaaagcagaa gaacttgtgg aacccatttt agtagggaac
    18541 gaagaagaaa taagaaatgc agcgcatgcc agaggaatca aaacatctag ctttacgatc
    18601 atcagccctg ataattacga taagtgggat gaaatggtgg atacttttgt tgaacgtaga
    18661 aatggaaagg catccaaaga acaagcagaa aagattttaa aagatgtgaa ttactttggt
    18721 acgatgctta cgtatatggg gattgcagac ggaatggtct ctggagcgat ccattcgact
    18781 ggcgacactg ttcgcccagc acttcagatc atcaaaacaa agcctggcat cagtcggaca
    18841 agcggagcga tgatcatggt acgtggaaga gatcaagaaa aatatgtctt ttcggattgt
    18901 gcgatcaacg tgaatccaac tgcgcaagaa ttggctgaaa tcgccgtaga ttctgcaaaa
    18961 acagcagaat tgttcgatat cgaaccaaaa gtagcaatga tgagcttctc gacaaaaggc
    19021 tcagcgaaat cgccagaagt agacaaagta gttgaagcaa ctaaaatcgc taaaaaacta
    19081 gcgccacaat acatgatcga cggcgaattg caatttgatg cttcttatgt tccttcagtt
    19141 gcacaactga aagcgccaga ttcagaagta gctggtcaag caacagtctt tgtatttcca
    19201 gaattgcagt caggaaatat cggctataaa attgctcagc gtttcggtaa ctttgaagca
    19261 atcggaccaa ttctacaagg cttgaacaaa ccggtttctg atttgtctcg tggagcaaat
    19321 gaagaagatg tatataaatt atcgattatc acagcagcac aaacattgat tgattaacaa
    19381 atacacgcga actagccaaa aacagcagct gctgttcttg actagttcat tttttcttta
    19441 aaaaaacttc attacgcgat atactggcaa aaagaaagaa gagcgggaga agatgaaaaa
    19501 tgatcgaact atcaggatta acaatgacag aacaattcgc agaggcaatt ggtcattccg
    19561 caatgccagg tgacaatctg atattgactg gtgatctagg tgcgggtaaa acaacgttga
    19621 cgaaaggaat tgcccaagga ttagggataa cgcaaatgat caaaagtccg acatacacga
    19681 ttattagaga atatagtcaa ggtcggattc cgttgtatca tatggatatt tatcgggtag
    19741 cagctagcgg agcagattta gggttagatg aatattttga aggtgatggg ctttccgttg
    19801 ttgaatgggg aaatctatta gaagaagcac tgccggagga ttatttggaa ctgattttag
    19861 aaaaaagtga tacagattta gaaaagcggt atgtgaaact aatctcatac ggggagcaaa
    19921 gtgaaacctt caaacagcga attctagaaa aatgggctga aaatcatgag tgaagaagtt
    19981 gggatcatct tgcgggaagc agtgccagga gatgcaaaag acattctttt gatgatggga
    20041 caagttaata aggagacaga atttttagtt ttggatgaag cagaactcct gttaccacca
    20101 gaaaccttag aagaagagtt ggattatata tacgaaagca ataataattt attgctgctt
    20161 gcaatctatg aagggacgat tattggaaca gcatctgtca aggcagacag tcaatttcgt
    20221 ttgtctcacg tgggagaagt cggtatcagt attctgcagg aatattgggg aatgggacta
    20281 ggcactctta tgctagaaga aatcatcagt tgggcgaagg aaatgggtgt actttttcga
    20341 ttggagcttg acgttcaagt tcgtaatgaa agagccgtac acctttaccg aaaaatgggc
    20401 ttccaaatcg aagcggtcat gcctagaggg gcaagaacag atctaggtga atttttagat
    20461 gtatataaaa tgagttattt gattgaatag aagaaatagg aaaagagtga gaaactgtca
    20521 tccttttgag aaggcagaaa aacacacgaa gggactgtga caaaagtcgt gtcgcagtcc
    20581 attttttttg aataaacggt gttcaatcag ctgaccttcg gtattagctc aaaaaacggc
    20641 aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa acgctgattt
    20701 cacaacctct tggtaaacgg tgtccaaaag cttgcatctg cggtaataag tccaaccaaa
    20761 tgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt
    20821 tttcacaacc tctccaataa acggtgttct cctgccagct cctcggtcac cagtcacaat
    20881 atttaataaa catgagaagc tattttttga aattgctcct tgtgactcgg agtgaaacgg
    20941 caggctcacg acctctttgt gtgtttcata agtattcttt tactttatag tcacaagttt
    21001 tttcaatgga tctgtcccga attttttttc ttgataaagc aggatttccg cacatgcacg
    21061 actatcttcc aatgcatcat ggtgatgatc taattggatg ttcaaatttt cgcacacagt
    21121 gttcaatcga tgatttggaa attcaggaaa caggcgacgg ctacttttca ctgtacataa
    21181 agaaagataa ttaggctgtt caagatgata ataatctaag catcccgcta agacctttgt
    21241 atcaaaagca gcattatgtg caacgactaa acgatttttt tgaaaatatg gttggatagt
    21301 ttgccagact tctgggaatt tcggcgcatt gcgtacgtct tcttgatgga ttccgtgaat
    21361 ttggacattt cgccagaaaa aaggagtctc aggctgtatc aatgtataga attcatcaac
    21421 gatctggcta ttttttacca tgactagcgc aagagaacaa gcactataag gctgatgatt
    21481 ggcagtttca aaatccatgg caacaaaatt catttctgta aaacctcctt gggaaattac
    21541 tgtaacatac ttttgaaaaa agcaaataaa tttaacaaaa aacatagaat tttttatgta
    21601 atatttgttg agaaaagcta gtttttgaac tcattccttg tgatttggag ttagatgggg
    21661 atcatgactt ctcggtatta agtcaaattt atgcaaagtt gtgaggaacc attttcaaaa
    21721 aaatttctta cgactcgaaa gtaggcgact ttttcacaac ttctccacta aacggtgttc
    21781 aatcagctga ccctcggtat tagctcaaaa aatagcaaaa tatgaggaac tattttgcca
    21841 tttgtttctc taatactcag gtcaggacgc tgatttcaca acctctccac taaacagtgt
    21901 tcaatcagct gaccctcggt attagctcaa aaaatagcaa aatatgagga actattttgc
    21961 catttgtttc tctaatactc aggtcaggac gctgatttca caacttctca ataaacggtg
    22021 ttcaaaagct tgcatctgcg gtaataagtc caaccaaacg aaaaatatga ggaactattt
    22081 ttgtttggct ggacttatta cttgatgcag tgcagctttt tcacaacctc ttaataaacg
    22141 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgaggaacta
    22201 tttttgtttg gctgttctta ttacttgatg cagtgcagct ttttcacaac ctcttaataa
    22261 acggtgttca aaagcttgta tctgcggtaa taagcccaac caaacgaaaa atatgaggaa
    22321 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctcga
    22381 taaacagtgt tcaaaagcca gtttctcggt attatgccaa atttatgcaa aatgtggaaa
    22441 gccattttca caaaattttt cttaatactc gaaactaaac gactttttca caacctcttt
    22501 atttcaaatc taattctact ggacaatgat cactgcccat aatgtccgta tggatctttg
    22561 catcagctag tcctggctct aaatctttgc tcgtcaaaaa gtaatcgatc cgccaaccag
    22621 cattgttttt ccttgaattg aatcggtagt tccaccaaga gtagacacct tcctgggttg
    22681 ggtaaaaata acggaatgta tcgataaacc cgttatccaa cagtcgagaa agagccgctc
    22741 tttcttctgg agtgaatcca gcattttttt gattcgtttt ccagtttttc aaatcaatct
    22801 tttgatgagc gacatttaga tccccacaca cgatgactgg tttttgtttt tttagatttt
    22861 ccaaataatt ataaaaggct tcttcccatt ctaatcggta atctaaacgt tttaattcgc
    22921 tttgagaatt tggcgtgtaa caggttacaa gaaagaaatc ggaatattcc aaagtaatca
    22981 gacggccttc tgtatcatgg atatcgatac ccattccgta agtggcattc aatgctggtt
    23041 ttttagcaaa aatcgctgtg ccggaatagc cttttcgttc cgcgtaattc caatattgat
    23101 aataacctgg aagatctaaa tcaatttgtc ctgcttggag tttcgtttcc tgcagacaga
    23161 aaaaatcggc gtctaactca tgaaaaactt ctaaaaaatt tttatttacg atggcacgaa
    23221 gcccgtttac gttccaagag ataaatttca ataaaagcac actccttcga attaatttcc
    23281 ttacctttgc gatactattg tacctcattt tagtaaaaaa tggtgtaaga tgaaagtaac
    23341 taaatgatcc aggaggaaag tagatgaaga ttggtgtaat cggtgtaggg aatatcgcag
    23401 aaaaagcata tttaccaact tatgcgaaaa aacaaggaac acttgatttt tattttgcaa
    23461 caagaaataa agaaacaaaa gaaagaattc aaaaagagta cgggttccaa catctatatg
    23521 aaacgatcga tgagctttta gaagaaaaaa tcgaagcatg tatgatccac gcagcaacaa
    23581 aagtacatta tgaattagcc aaaaaatgtc tagaaaatgg tatccatgtt tatattgaca
    23641 aaccactgag tgtccatcta aatgaagttc gtgaattgca agaattagct gataagaacc
    23701 aaataatttt gatgatcggt ttcaaccggc gctttgctcc tatggtagaa gaattgaaac
    23761 gtatcccaga taagcggttg atccacctgc aaaaaaatcg aatagcggca aaagaaacga
    23821 cagagttcgt cctttatgat ttgtttttac atcttattga tacagctgtg tatttattag
    23881 atgaacccat tttgagaaca aaataccaga taagagaaac aaaggaattt ttggaatatg
    23941 ccattttaca attagaaaca caaaatcaaa cggctatttt aacgatggat ttagcaagtg
    24001 gcgctaatat agaaaaatat caagtaacaa gtaaaaatgg tacttatgaa gtaaacgatc
    24061 tgacccagct tgtcattcaa gatgctgatg gaaaaaaagt gaaagaattt ggcgactgga
    24121 caaacacgct agtcaaacgc gggtttgagc caatggtaga ggccttcttt tctcagatcc
    24181 gtttaggtaa atcggaaaca gaattgctta aacaaaaaga agttgtaaga agtcatgaat
    24241 tatgtgagga agtgcttaaa gatcactacc gtcatcaact ttgaacaaga ctggcaagat
    24301 tgtattaagc gtgatagaat aatgaggacg tttgagaaaa gaaaggaaaa tcctcagtga
    24361 ataaagaaga aatagttgaa agcttagcca cgattactat attgtttgat gaaccattga
    24421 tgaactatac gtttacgaaa acaggtggac cagctgatgt ccttgcattc ccgaaaaaac
    24481 aagaagaagt aaagcaaatc atcgattatt gccgaatcca tgatatacct tggatggtat
    24541 taggaaatgc aagcaatctg atcgttcaag atggcgggat tcgcggtgta gtcatcatgc
    24601 tgactgaaat gaaacagatt catgtcaaag gaacgatggt cattgcggaa gcaggtgcct
    24661 cactgattga tacgacttat gcagctttgg cagaatcatt gactggattt gaatttgctt
    24721 gcggcatccc gggaagtgtc ggtggagcgg tttatatgaa tgcaggtgcg tatggcggag
    24781 agatcaaaga tgtttttgct gaagtggatc ttttgcttga agatggaaca ttgaaaactt
    24841 tgacaaaaga cgaaatggta ttttcttatc gtcacagtaa agtccaagaa ttacgtgcta
    24901 tcgtattgga agctcgattc agtttgcaaa caggggatta tgaagcaatc aaagcaagaa
    24961 tggatgagtt gaccgaattg cgtcaatcaa aacagccatt agaataccct tcatgcggaa
    25021 gtgtctttaa gcgcccagta ggacactata caggtcagct gatccaacaa gcaggattgc
    25081 aaggcctgaa atggggcggt gcacaagtct ctgaaaaaca tgctggattc atcgtcaata
    25141 tcgatcatgc gacagcgaca gattatattg aattgattgc gcacatccaa gaagtaatca
    25201 aagaaaaatt tgatgtgtct ttagaaacag aagtgcggat cattggagaa aaacaagcaa
    25261 tatcagaaac aagcagaagt ttagccaatt aataagtaat ggaacttaaa ataagtaaaa
    25321 gagcaggacg gtggccgtcc tgctcttttt tgtggttgtt tacgcaaatg gcgcaaatct
    25381 tgtacatttt aacacaataa aacaaaaaat atagaatatt tcctcataaa tttttccatt
    25441 ttttgcaata ttctcaaaaa aaagataaaa aatgagaata actgatgaaa aaacaagcat
    25501 aatccatcat aaaatcagta taggtgaaaa gatgaacttt catctgcata tcaagtaatt
    25561 ggtatgaggc attttttgag gtgtaggata tgaagttgga ttaccttata gaagaggaag
    25621 atagagaaaa actgctgcta gctagatatt tagaatttga gaaaaagaat tatgtagtgc
    25681 tgcctaagtt atatgaaaca ttaggtttgt ctaaatttaa aacaagaaat tatatcatag
    25741 aattaaataa agaatttact gaattcaaaa caaagccaga gatccaaatt tcagaagaag
    25801 cagagatcca tgtcgaacgt ctgaatttga cggatatcaa aaatttaaga agaatctact
    25861 ttcaacgatc aaaaattgct cgactgttaa cggagatcgt ggattcaaaa gtaacgatcg
    25921 aaaaatttgc agagaatcat tttttaagca tatcaagagc ctatgtaaaa agaagagaat
    25981 tagtctcttt tcttgaacag catcaaataa agttaaagaa aaacatatta gttggggaag
    26041 aaataaacat acggaacgta ttatttgggg tatatttcgg ggtctttaat ggtttttcta
    26101 tgccatttag agaaagcgtc atgaatgaag caaagagtat cgtgcaatat tttgaatatc
    26161 tgttcaattt aaaactgtct gcaacaaaac gaatcaaatt caatttgcta accactatta
    26221 gtttgttgag ggcctatggg aatcaaccaa tcggaaaacg tttcttttca gatgaatact
    26281 ttaagagctc agaaggaatg aagatcatca aggatttatc acatatttct tttcttcata
    26341 tccctaaaga gcagatcatt gatgagattt cttatatcat gttgtttttg atcacggaag
    26401 aagaatttga attagatggc gcatttggac ttgcggaagc tcattttcat gatatccaca
    26461 tgattagtca aaaaatgtca gtggaattga ttgatcaatt accattttcg attgaaatct
    26521 ctgaagaaga aagactatat atacgaaaag aacttcgtat gggtatgatg catgtcaatc
    26581 gaaaaaattg gatattcgac tttgagatga ctagctttac gtctaaaaag cagctacagt
    26641 ttttcttcga gacgtatcca atattcagta cagcaatcca aaaggtcata caagagaata
    26701 aacgctattt cttgggaaaa gatgaacgtt tcatgattcg cttattttat gattatctct
    26761 ttttattggt cggtatttgt ccggtatcat attttgaagc accgattcat gtctgtattg
    26821 atttttcgca aggatctgct tataccgatt acattatttc tcaagtgaaa ggatttaaaa
    26881 attacaatat tgtgattgag agtcgaatca cttctcgtac gaatatttat ttatctgatt
    26941 gtttacttga aaaatttaat aaagaacaga taatttggaa aaatccaccg actccagatg
    27001 actgggaata ttttggaaat accatcatca gagaaaaaaa tagacattta actgaaaaaa
    27061 tcagctagta tttatggatg gcagctttgt ctcacatatt tattagctga tttatgtttt
    27121 tttcaaagaa aaggtaacta gatataacta gatataacta gatatattac ttaacaaaat
    27181 taaaaagtac ataagttatt attccgattt tcttgaaaca ataaaaaatc aaaaaaaata
    27241 ctctcctaat agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa
    27301 ttactacttc atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac
    27361 gttcaaaaca actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa
    27421 aacactacgt taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata
    27481 tgcatgtacc cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg
    27541 agaccactga ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc
    27601 tacttcctgc attgacttcc tataataaag tcagttatcc tccaacgata aaccactact
    27661 ggttaattcc taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta
    27721 tcttttccga tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt
    27781 tgaatcatgc tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg
    27841 aaaaagatta ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt
    27901 cgatatctga tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca
    27961 aagtaatttc aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt
    28021 ccttgtccta ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg
    28081 ttcgtgctga tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt
    28141 acgcccctaa agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag
    28201 aagaaatcaa aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat
    28261 taacttatat agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag
    28321 aagcatctgc tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag
    28381 aacttcaagc agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg
    28441 ctgaattttc tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag
    28501 tcacttctat ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg
    28561 agatccttga agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa
    28621 ttacctttac ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg
    28681 ttcatccaaa cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag
    28741 gtaaacccgt accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg
    28801 gttatttgac caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt
    28861 cactccaaga actccatgct cagtcactat tacatgtttt accattaaac aaacagatag
    28921 atcaaaaaga acaaaaaaga caagaagaaa gcttgtcaga aaaaaaacaa caaatggctc
    28981 atgcagtttc tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat
    29041 ctagaaaaat ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag
    29101 tttctggatt ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat
    29161 ttgttgctaa acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa
    29221 aacgcttgtc aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta
    29281 gtccgggaac acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc
    29341 gaggtatggc aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt
    29401 tctgatttat atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc
    29461 ataatggaga cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc
    29521 tggatgacaa gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa
    29581 tttcgatttt atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg
    29641 gaatttcaaa cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct
    29701 gaagaacttg aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc
    29761 tgtaccgctt ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca
    29821 gtgaaaccag attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa
    29881 gaatttccta ccctccatac ggacggacca atctatcttc acacacagac atggagccat
    29941 caggcgattg ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc
    30001 acacctaatc cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga
    30061 aatggtgaaa taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt
    30121 acttttttga gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg
    30181 ggcagtgaca aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt
    30241 gattttttta ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata
    30301 gaggtaacca aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat
    30361 agtatccatg attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc
    30421 atctgcattt tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt
    30481 tccgtcttta cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc
    30541 gaattgcagt aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat
    30601 cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt
    30661 tttattgagc aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc
    30721 atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac
    30781 tgttgaacta aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt
    30841 taatatcttt tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat
    30901 tgtatttccc cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg
    30961 taataaaata tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa
    31021 ggttttcaaa tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc
    31081 gtgtgtttac caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc
    31141 acttaatata gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat
    31201 taacagccgt tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcac
    31261 agacaagttc tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat
    31321 atacagatcc aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg
    31381 cagggatggc agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa
    31441 agatgcctgt tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa
    31501 caaaatttca aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc
    31561 taaaaggtgg tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg
    31621 ctgatgcggt cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg
    31681 gagggatgag tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact
    31741 tagtggaggg gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag
    31801 atgtgacaga cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag
    31861 gagaagaacc aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg
    31921 ccagcaaaga atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc
    31981 aagaaggatc aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg
    32041 atatggataa aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg
    32101 aggttgttcg cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg
    32161 aaatggatac aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt
    32221 atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa
    32281 aaggatatgt cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa
    32341 aagaaacact agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg
    32401 ctcaattcaa ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga
    32461 tcaaaatcgc accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg
    32521 aagtattaga taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag
    32581 gtggcttaca tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg
    32641 ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt
    32701 aaataaaaag aggttgtgaa accagcgttt tgagctaata ccgagggtca gctgattgaa
    32761 cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
    32821 gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
    32881 tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
    32941 gctctaaaca cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt
    33001 cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt
    33061 ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt
    33121 ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat
    33181 gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata
    33241 aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc
    33301 agttggacac gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct
    33361 agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag
    33421 taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt
    33481 acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta
    33541 caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt
    33601 ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt
    33661 tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct
    33721 gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca
    33781 ggcatcacag catattcggc ataaccacct agattgacta atccgtatac ttttgtccca
    33841 ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc
    33901 aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg
    33961 acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact
    34021 tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat
    34081 ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag
    34141 tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac
    34201 tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt
    34261 aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg
    34321 tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca
    34381 ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt
    34441 ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc
    34501 ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc
    34561 cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg
    34621 ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat
    34681 caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct
    34741 ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg
    34801 ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca
    34861 tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac
    34921 cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg
    34981 ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag
    35041 tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt
    35101 ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc
    35161 tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa
    35221 aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa
    35281 agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt
    35341 tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat
    35401 agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt
    35461 ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc
    35521 taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg
    35581 atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt
    35641 cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt
    35701 tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg
    35761 cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt
    35821 tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg
    35881 cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac
    35941 gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa
    36001 gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca
    36061 gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc
    36121 tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga
    36181 ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca
    36241 atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc
    36301 aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt
    36361 tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt
    36421 gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg
    36481 ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt
    36541 gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc
    36601 gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc
    36661 cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt
    36721 cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt
    36781 ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt
    36841 ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt
    36901 gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag
    36961 gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt
    37021 tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat
    37081 agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta
    37141 cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg
    37201 caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc
    37261 cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt
    37321 tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca
    37381 tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc
    37441 ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac
    37501 gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg
    37561 ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat
    37621 aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg
    37681 ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga
    37741 cactcctttt aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt
    37801 ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc
    37861 aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat
    37921 ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg
    37981 gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca
    38041 caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga
    38101 gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact
    38161 ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat
    38221 catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg
    38281 aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg
    38341 tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc
    38401 ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa
    38461 caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa
    38521 tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt
    38581 agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg
    38641 aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat
    38701 agctggattc accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga
    38761 tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca
    38821 tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga
    38881 tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg
    38941 ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt
    39001 aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg
    39061 tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt
    39121 tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa
    39181 agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt
    39241 cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct
    39301 agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc
    39361 agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact
    39421 agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga
    39481 tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt
    39541 aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga
    39601 attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc
    39661 aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta
    39721 atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta
    39781 tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt
    39841 ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg
    39901 aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt
    39961 aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat
    40021 tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca
    40081 acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga agagatgaat
    40141 ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga
    40201 gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct
    40261 tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga
    40321 acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt
    40381 gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga
    40441 ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta
    40501 ttcttatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg
    40561 aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg
    40621 atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc
    40681 atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg
    40741 ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc
    40801 ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat
    40861 attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac
    40921 gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat
    40981 atcattttgc cttatctttc tccaggaatc atagctggat attttatggc atttacctat
    41041 tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca
    41101 gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg
    41161 ttagttttct tgttctccat gatattggtc attggatact actttattag taaagaaaac
    41221 cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga
    41281 aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg
    41341 ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact
    41401 ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag
    41461 tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga
    41521 caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac
    41581 tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt
    41641 tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag
    41701 ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt
    41761 ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc
    41821 tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag
    41881 cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca
    41941 aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg
    42001 atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt
    42061 ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca
    42121 tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta
    42181 caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct
    42241 atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc
    42301 taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact
    42361 cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg
    42421 agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt
    42481 tttctaccac gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga
    42541 gacgggctag taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat
    42601 gcaaatatac ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa
    42661 tatttgtagc tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa
    42721 attattttgt cagatatctg aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg
    42781 gaatacaaat atagaataga catattgcag ccgatagttt attatgcttt tagacacatt
    42841 aaactggagg aaaaaatgaa aaagagcgct aaagatcgag tttttgatga gctgatacga
    42901 ctaacaggga atatgtcttc tgtcactgcc gagaaaattt cgaagaatct aggtatctct
    42961 cgtcagaatg ccagccatta tttgacgaga ttagtggaag ataaaaaggt cgagaaatta
    43021 ccgggaaagc ctgttttgtg gaagccttta gatgaatatg cggttgtaga taatacggag
    43081 caaataaacg aagcctttca ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt
    43141 caaaaatgta tagccgctgt caaatatccg cctaacggac tcagtgtatt aataaatgga
    43201 gccacgggtg ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc tatccatgag
    43261 caaattattg aaaaagatgc accatttgct atcttaaact gtgccgatta tgcggacaat
    43321 cccgaactgc tttcggcaac actgtttgga tacaagaagg gggcctttac gggggctgaa
    43381 aaggataccg atggtttatt ggcaacggcc aacaacggct atctttttct cgatgaggtc
    43441 catcgcctat cgaaagaaaa tcaagaaaag ctctttctat ttattgatac tggaaactat
    43501 cgtccagtcg gtgaaaatgt aaattggcat tctgctaagg ttcgttttat tttcgccacg
    43561 acggaaaaag gtgaaaatta tttattagac acctttgatc ggcgtattca aattagtgtt
    43621 atgttgccaa cttttgaaga tcgccccatt cgagaacgat tagagctgat tcaattattt
    43681 tttcaaaatg aagcggatgt tttacaaaaa gatattattg tttcaagaga agccttgtct
    43741 ttaatgttat ctcatccctt tagtgggaat attggtaagc tgaagaatat cattaaaatc
    43801 agttgtgcgg atgtgtatag tcgaacacaa gaagaaccat taacgattgg gaaaaatgag
    43861 atcttgattc agttaaatat gctggaatcc gaagtagaaa gcttgccgat tgctagttta
    43921 ttgataaaga aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga ttatgattcg
    43981 attttggaaa aaattactca gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg
    44041 ataaagaatg aagtacagca tttggtgcat cacattgcta aggattcttt tttatcaatg
    44101 agtagaaaat tgatgattca gatgtttaga aaggtctggg tatccgtttt aggaaaaacg
    44161 tacggattga cgacgagtgg acctgttgca gaaattatta gcagatccta tgtcatttcc
    44221 ccaagctata agacagactt aaccgggata aatcgtacat tagctgcaaa tctacctcgg
    44281 acgacttact tatgtaatca gttcttggag catttaccac cgctctcaga aaaggacgag
    44341 aactttctaa gactgcttat tgtcgtgact ttatctgatt atgtagatga atccatcgag
    44401 ctgaagggcc tgttgcttgc tcacggagat agtacggcta gcagcattca agcagtagtg
    44461 aatcaattat gcggcaatta tgtttttgaa gcgttggata tgccagtaga gagtaatgtg
    44521 tcagagattg tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac agatagtcta
    44581 gtgctgatcg tagatatggg ctctttaaat caaatttaca cacagatcaa gaacgattta
    44641 aaaggggagt tacttcttct gaataatttg acgacttcca ttgcacttga tattggcttg
    44701 aagatgacga tgaatgcgcc atttaaacag attgcggagg atgcagcaga caagtattca
    44761 atcaatgttc aatactttga aggcttttct caaagtagca acattatcat ttcctgtatg
    44821 tcgggtttgg ggatttcgga caaattgaaa gatgtttttc gtcatcattt atcggatgaa
    44881 tccatcgaag ttttcacgaa ggactacaga gaactacgtg aattaatcga ccaaaatgat
    44941 gaaacttatt ttgacaagac aaaattggtc atcacgactt cggatcttcc acgttccttt
    45001 tcaattccta atattaatgt ttatgacatc ttagacggtg atggagcagc caatttggag
    45061 catattttga gtcctgaaat gaagccggca gaatttaacc gcttgattca ggagctagta
    45121 aaattctttt cgttggaagg aattgcggac cgtttgaact ttctgaaccc aacgatcgtg
    45181 atgagtgaag tggaaacagt catttctaaa tatgaaaatt actatcattt cactttaaat
    45241 ggccgtgtaa aactaaatct ctatatgcat acggcattga tgatggaacg tttattcttg
    45301 gctcggacag aaaaatctga agaaccgaat tatgatttga aaccagatga agatgaattt
    45361 tattatgttt ctagaagtat ttttcaatca atggagatga aatataattt taaagtcaat
    45421 gcttatgagc tttcgctgct ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc
    45481 taaaaaagtg tctaatctgt tttttttagg ttagacactt tttttaatgt gaattaacgt
    45541 gattaaacca atgtttttaa aagttggcac aggaattgct tatataaaag tgaacagaaa
    45601 gaaaggaatt aaccatatga agaaaattct aatagcgact cacggtcact tagcttcagg
    45661 ctttcttagc agtattcaat tactgacggg gaaggttcaa gagattacgg tgattaatgc
    45721 ttatgtggat gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc aggtgaacga
    45781 gcaggatcag atttttgttt ttaccgattt atttggcgga agtgtcaatc agaagattac
    45841 caaaaccttt ttggagaaag agattgctgt aactattttg tctggcttta atctgccaat
    45901 tctgctcgaa atagtattgg caaccaagga attaacaaag gaggatattc aggaactggt
    45961 aaataaatgc caacaggaat taaagatgac cattcttgat gaggtacatg tagtaaatga
    46021 agaggaagat tttttttagg aggaagaaaa atgattaaag cagtgcgaat tgatgaacga
    46081 ttaattcatg gacaagtagc gatgacttgg acaaagagtc taaatttaac cgggttagta
    46141 gtagcaagcg atgaggcggc taccaacgat attcaaaaga tgacattgaa gatggctgca
    46201 ccatcgaatg tcaaagtcat tattaaaaca gttgatgacg cgattcattt gttaaaagat
    46261 ccgcgggcga aggacatgcg cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa
    46321 gttgcgaaag ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg
    46381 acaccgccat cggaagataa gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa
    46441 attcaagact taaaagaatt agttgaactt tatccagata ccgtttttca gcctacaccg
    46501 gcgatggaaa aacgttcagc tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa
    46561 aaattatgta tgatgcattt gtagtcgctt tggccgtttt tattggagtt gctggacatg
    46621 aaatgttcgg gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt
    46681 tgatggggga tttgcagacg gggttgattg tcggggcgtc actagaagcc atttttatgg
    46741 gtgtcgtcaa tatcggaatc tctagtacgg cggagccttc tttggctgct gggttggcta
    46801 cagcgttcgc aatcaaaatg ggaggaaacg tggatgcgat tattcccatt gtctttccac
    46861 tagcagtatt aggattacag ttaatgaata tgattttttc ttttgtatgt ggaccgatgg
    46921 ctgctaaatt tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact
    46981 tcgttatgtg gtttgtccac tatgcgcttt atgctcttat tccattcttt gccgttttat
    47041 ttggagcaga tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc atgaacggat
    47101 taacagtcgc tggaaatctg ctgccggcag ttgggatggc gatgctgtta cggatgcttt
    47161 ggagtaaaaa tattgctgtg tggtttttct ttggggccgt tgttatggcg tatctcgagt
    47221 taccattgat tgcaattgcc gcagttggaa cgattattgc aattgttata gcccaaagag
    47281 atctacaact aaaaaatata tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa
    47341 cgcttgacca agaagaggag gatttctttc aatgaaatta gctgatggaa tttctaaaga
    47401 tgagaaaaaa ttggttcgga agatgttttg gcgctcgatg accatgtatg cttcggtgaa
    47461 tccggtgacg atgggcggcg gtggtttcgc ctattctatg atgcccttca ttaatcattt
    47521 ctacaaaagt gaagaagatc gagctgcggc gctggaacgt catacacagt atttttcgac
    47581 gaccattcct tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga aggaaaattc
    47641 tgaaaaagct ggttttgacg tggaaagtat caacgcgatc aaaacgagtt tgatgggacc
    47701 attggctgga attggcgatt cattgttctg gggagtttgg cgtgtcgtta ccgcgggact
    47761 agcgatcaac ttagccaatg ccggaaatat tttggcacca attatctttt tagttatgtt
    47821 caatatacct aattatttga tgcgatacta tggcggcgtt ttaggctatt cattaggctc
    47881 taaatatatt gaaaagctgt atgcgaacgg gttaattgaa attctgacaa aagctgctag
    47941 tatcttgggt ctgatcatgg taggcgcaat gacgatcagt atggtcaaat tcaataccgt
    48001 tttgagcttt tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc tcgatagtat
    48061 ttttaaagga attgtaccgt tattggtaac attgggctgt ttcaaattac tagataaaaa
    48121 agtcggcgtc atcaaaatta tttttggaat ggtcgtcttg ggaattttat tgtcattact
    48181 tggaatcgca tagtaggaag ctgcagctac taggctgcag tttttttagc aaggagggaa
    48241 aagatgacta ttaatgaagt aatttattca cccaaacacc cattatttaa tgctgagtta
    48301 aacgacggaa atgtgactac ttcaaacgaa tacagctact atcggaatat tcctgatgta
    48361 aagggattga cctttgatgt aagtaatcaa aagacattag gaaaccttga tgtttgcggg
    48421 acgattcaaa cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt
    48481 tgggtcaata aacagctttc tcaaaccaat tcgttatcct taaaggttga agtaaacggg
    48541 attgtctacc atttgtcaga atcggatcat cgtattgaag tcgatgtgat tcaagactgt
    48601 ttgcctagat atattcatca ttattcgacg tttaaggtca ttgtagtatc ctttgctcca
    48661 atactggcta ataagcgttt aagtatgctt gttcaacagg tttacattgt gaatgaaaca
    48721 cacgaaagcc ttcaagtttc agtgaaagat gtgcctttgt accaagagaa gtattcggat
    48781 cagcaaaatg tgttgatttc tcaaaaagga aataaggaca cgatcgcaca acaggaagtg
    48841 gcaagcttcg ccattgcttt tgtggaccca aatgcttttg aagaagtaat gacttttcag
    48901 gaatctgata tcgaaaaatg gctgaaggaa acaatgaact attttgctca gctatttggg
    48961 cagctgtcaa tgtcagacga tagagttgtc catctctatc gtcgtgcact ttatcaaagc
    49021 ttttctagtt tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg gggcagctat
    49081 cctgctacta atcgaatttg gaataaggat atgtattacg catcattgcc attcaccatg
    49141 tttgaaccgg agttatgtca aaaaacggtt ctatggtttg atcaatatgg cgttaagttt
    49201 cctggttcaa aatttacagg aggtatcaat cattctctga gtaattcgtt atccagtgcg
    49261 ttactggcta gtctttattt tgaacatact ggggatacaa aattctttga gcggtgtccg
    49321 caggttctac tgaatgcttc aagaattatt gaggacatat tagctcaacg tcggtcaggg
    49381 gaaccaatgc tttttccatc tgtttggtta tcagacgcct ttgcacttgg aaagtatcat
    49441 accggcagca atctttgtct ttggaaggcc tgtagtggac ttgcagaaat ttttgaagcc
    49501 ctagaacaac tgagtcttag cgagcgatac aaagacatcg cctgtaaact aaaggaatca
    49561 atttgtaaac aaatgaccat agatggtact tttggggagc aatttttgga ggggatcggt
    49621 gatgaggaga aaacaaccta ttcagtgaag aactatcaaa aaccgattct tgagcaaggg
    49681 ttaatctttt taagtgatgt gattgtggat gggaaaatca atttattgat gcatgatgga
    49741 gaagaatcag acactacgtt gatgcctttt tatcaatttt tagataataa agatccttta
    49801 tatcagaaca ccatgacctt ttctgctagt tctttcaatc caacttatag cgaggaaatc
    49861 aaaggcatca cttggggatt agaatccggc gccacttttc ctggctttat cactgtttta
    49921 atgagtgaca tgcacaacca tcaagccttc gtaacccgat tagaggagct gatttgtttg
    49981 gcagatttag acggttcatg gtggtggtgg ccgtatcgtt tagaggctaa acaaggagaa
    50041 gttgtgcgag actttggctg cggcaaatgc ggctgggcat cgggcctttt tgtcagtata
    50101 atgatcagtc aatatttcgg attgaaattc aaaaaaggag tattgcaaat tgatcctgtt
    50161 gataatttaa cgttttcttg gaaaaatttg aagttcggtc aagccttcat ttcgattgaa
    50221 tgtacgcaat ccgagttgag tatttcaaat ttgtcagaaa agccattgaa aataagtgat
    50281 acgaaaaaaa ttcttcacgt agaaaaggga aaaacgattt acattcaaag gagaaaaaga
    50341 tgagtatact aaaaaatatt acccaattta aaaaaatcca gagtcgagca atcactcccg
    50401 aaaatccaac aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc
    50461 gcaaaggtcg tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt gcggatatta
    50521 ctggaacggg aataattcgt catatgtgga tgacgattcg tgatcgaaca gaatttggca
    50581 gttttgtttt acgtgattta atcttaagaa tttattggga tgggtcggat agcccggctg
    50641 ttgaaagtcc tttaggggat ttcttttgta atgggtttgg agagcgttgc gaggtgaact
    50701 cgttgcctat agtggttaat cctacggggg gctttaattc ctatttcgaa atgcctttcc
    50761 aaacatccgc taaaattacg atcgagaatt tacatccgaa ggatatgtcc tcattttttt
    50821 atacgataaa ttacgctttg gtggacgaat tggaagaaaa tagcctatat tttcacgcaa
    50881 attggcatcg ggaacgcatt accaaattac aggaggatta taagttaatt gatcgattaa
    50941 agggtcaagg ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa cgttattggt
    51001 ggggcgaagg cgagtttaaa ttctacattg atggggatag tgagtatccg accgtaacct
    51061 caacaggttc agaagattat tttggcgggg cgtgggcctt tcatgagtac gatgagttcg
    51121 gacgtccaca tgcgaaaaac tattcaacag cctttttagg gtatccttac caatcaaata
    51181 gagatgccac aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg
    51241 acgacgcaat gccacgacat ggtctttatc gttggcatct tcaagacccc atctgtttta
    51301 ccgaagattt atcggtcact ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc
    51361 ggcaagacga tgttgctagt gtcgcctatt ggtattcaac agaagcttca ggaaatacta
    51421 aacgtcattt cactgatcga gaacgcttgc cgcgttaatg aagtgttttt cacatattcg
    51481 aagggaggaa cctatgatga tatatggggc aattgaagct ggagggacta aatttgtctg
    51541 tgcagtcggt actgaggaat taacgatttt ggaaagagtc agttttccca caacaactcc
    51601 agttgaaaca atgaagcagg tcattgattt ttttaaacct tttaaggaac aattggcagg
    51661 catcggggtc ggctgctttg gtccaattga tattcaatgc aaatcaacga gtttcggtca
    51721 tatcacatca acaccaaaac ttgcttggca aaatttcaat tttgtaggaa cgctaaagca
    51781 atatttcaat attccgattg aatggacaac agatgtcaat gcggcttgct atggtgaata
    51841 tgttgctggt tctggcaaag ggttagcagg tgttgcatac ttcactgtgg gaacaggtat
    51901 cggaggtggt gccttgttca atgggacttt cgtggaaggg ttcagtcatc cggaaatggg
    51961 gcatatgttg atcaagcgac atccaaagga caatttttca ggaaactgtc ccttccacca
    52021 agattgttta gaggggatgg cagcaggacc agcgattgaa aaacgattgg gtgtaaaagg
    52081 ccagaattta ttggctgacg attcgttttg gcagattgaa gccttttacc ttgcccaatg
    52141 tgcatacaat acaacactga tgctttcgcc agatagaatt atttttggcg gtggtgtaat
    52201 gaaacaagaa cacatgaaga aaaaagttca ggataaattt gttgagctta taaatggata
    52261 tgttgaaata ccaccaattg atagctacat tattacgccc gaattaggtg ataatgccgg
    52321 aataatcggc ggattagcat tggccagaaa agcggtaaga aataagcaac cataattaat
    52381 gaatgtttca ttgatactca tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga
    52441 agttggataa agcgaaaatg aattcaaata aaaaggctct aagtcaaatc gtgttgtttt
    52501 cattaaatca ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt
    52561 ataccttgag gatattcact ggtggcgtac acttaaatag taatatattg tgttacttta
    52621 tgtctgtggt aatagttata gttgcttacc aatgtatgtt catttttcga aatgttgact
    52681 tcataatcct tggcttaatg tttgaatttt tttggtgata gattttttca gccgtataaa
    52741 ataaatcgta attctattat agagagttgt gattataaaa agatagcaag aaaaaaaaga
    52801 aagtttctat acattttaaa tattttattc ctatttctat tgctaaaaaa gtagaaattc
    52861 tatatttacc tttaactctc gcttctct
//
LOCUS       DACDBG010000011        50535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDBG010000011 DACDBG010000000
VERSION     DACDBG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.011"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..829
                     /locus_tag="IVZ90_000635"
     CDS             <1..829
                     /locus_tag="IVZ90_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8261706.1"
                     /translation="ELTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSI
                     TAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDR
                     KSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSV
                     QADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHK
                     LRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(917..1309)
                     /gene="rpsI"
                     /locus_tag="IVZ90_000636"
     CDS             complement(917..1309)
                     /gene="rpsI"
                     /locus_tag="IVZ90_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8261707.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1325..1768)
                     /gene="rplM"
                     /locus_tag="IVZ90_000637"
     CDS             complement(1325..1768)
                     /gene="rplM"
                     /locus_tag="IVZ90_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8261708.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2287..2754)
                     /locus_tag="IVZ90_000638"
     CDS             complement(2287..2754)
                     /locus_tag="IVZ90_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8261709.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2874..4412)
                     /locus_tag="IVZ90_000639"
     CDS             complement(2874..4412)
                     /locus_tag="IVZ90_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8261710.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4492..5178
                     /locus_tag="IVZ90_000640"
     CDS             4492..5178
                     /locus_tag="IVZ90_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8261711.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5379..5906)
                     /gene="msrA"
                     /locus_tag="IVZ90_000641"
     CDS             complement(5379..5906)
                     /gene="msrA"
                     /locus_tag="IVZ90_000641"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8261712.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5990..6667)
                     /gene="pgmB"
                     /locus_tag="IVZ90_000642"
     CDS             complement(5990..6667)
                     /gene="pgmB"
                     /locus_tag="IVZ90_000642"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8261713.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6664..7794)
                     /locus_tag="IVZ90_000643"
     CDS             complement(6664..7794)
                     /locus_tag="IVZ90_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8261714.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7794..9977)
                     /locus_tag="IVZ90_000644"
     CDS             complement(7794..9977)
                     /locus_tag="IVZ90_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8261715.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9974..10999)
                     /locus_tag="IVZ90_000645"
     CDS             complement(9974..10999)
                     /locus_tag="IVZ90_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8261716.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11044..11871)
                     /locus_tag="IVZ90_000646"
     CDS             complement(11044..11871)
                     /locus_tag="IVZ90_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8261717.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11868..12935)
                     /locus_tag="IVZ90_000647"
     CDS             complement(11868..12935)
                     /locus_tag="IVZ90_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8261718.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12961..13797)
                     /locus_tag="IVZ90_000648"
     CDS             complement(12961..13797)
                     /locus_tag="IVZ90_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8261719.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13794..14663)
                     /locus_tag="IVZ90_000649"
     CDS             complement(13794..14663)
                     /locus_tag="IVZ90_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8261720.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14733..16016)
                     /locus_tag="IVZ90_000650"
     CDS             complement(14733..16016)
                     /locus_tag="IVZ90_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8261721.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16030..17715)
                     /locus_tag="IVZ90_000651"
     CDS             complement(16030..17715)
                     /locus_tag="IVZ90_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8261722.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17990..19015
                     /locus_tag="IVZ90_000652"
     CDS             17990..19015
                     /locus_tag="IVZ90_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8261723.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19610..23263)
                     /gene="rpoC"
                     /locus_tag="IVZ90_000653"
     CDS             complement(19610..23263)
                     /gene="rpoC"
                     /locus_tag="IVZ90_000653"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8261724.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23342..26959)
                     /gene="rpoB"
                     /locus_tag="IVZ90_000654"
     CDS             complement(23342..26959)
                     /gene="rpoB"
                     /locus_tag="IVZ90_000654"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8261725.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27687..28655
                     /locus_tag="IVZ90_000655"
     CDS             27687..28655
                     /locus_tag="IVZ90_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8261726.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28673..29578
                     /locus_tag="IVZ90_000656"
     CDS             28673..29578
                     /locus_tag="IVZ90_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261727.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29575..30381
                     /locus_tag="IVZ90_000657"
     CDS             29575..30381
                     /locus_tag="IVZ90_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261728.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30538..31506
                     /locus_tag="IVZ90_000658"
     CDS             30538..31506
                     /locus_tag="IVZ90_000658"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8261729.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31823..32347)
                     /locus_tag="IVZ90_000659"
     CDS             complement(31823..32347)
                     /locus_tag="IVZ90_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8261730.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32634..34445
                     /gene="scm"
                     /locus_tag="IVZ90_000660"
     CDS             32634..34445
                     /gene="scm"
                     /locus_tag="IVZ90_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8261731.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(34600..35586)
                     /locus_tag="IVZ90_000661"
     CDS             complement(34600..35586)
                     /locus_tag="IVZ90_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8261732.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(35609..36175)
                     /locus_tag="IVZ90_000662"
     CDS             complement(35609..36175)
                     /locus_tag="IVZ90_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8261733.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(36189..36605)
                     /locus_tag="IVZ90_000663"
     CDS             complement(36189..36605)
                     /locus_tag="IVZ90_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8261734.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(37062..38996)
                     /locus_tag="IVZ90_000664"
     CDS             complement(37062..38996)
                     /locus_tag="IVZ90_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8261735.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            39298..40233
                     /locus_tag="IVZ90_000665"
     CDS             39298..40233
                     /locus_tag="IVZ90_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8261736.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            40323..41426
                     /locus_tag="IVZ90_000666"
     CDS             40323..41426
                     /locus_tag="IVZ90_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8261737.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            41439..42392
                     /gene="menA"
                     /locus_tag="IVZ90_000667"
     CDS             41439..42392
                     /gene="menA"
                     /locus_tag="IVZ90_000667"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8261738.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(43327..44673)
                     /gene="gor"
                     /locus_tag="IVZ90_000668"
     CDS             complement(43327..44673)
                     /gene="gor"
                     /locus_tag="IVZ90_000668"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8261739.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(44807..46048)
                     /locus_tag="IVZ90_000669"
     CDS             complement(44807..46048)
                     /locus_tag="IVZ90_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8261740.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(46066..46989)
                     /locus_tag="IVZ90_000670"
     CDS             complement(46066..46989)
                     /locus_tag="IVZ90_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8261741.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(47210..49702)
                     /locus_tag="IVZ90_000671"
     CDS             complement(47210..49702)
                     /locus_tag="IVZ90_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8261742.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(49709..50176)
                     /locus_tag="IVZ90_000672"
     CDS             complement(49709..50176)
                     /locus_tag="IVZ90_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8261743.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(50312..50385)
                     /locus_tag="IVZ90_000673"
     tRNA            complement(50312..50385)
                     /locus_tag="IVZ90_000673"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50349..50351),aa:Arg,seq:acg)
     gene            complement(50443..50516)
                     /locus_tag="IVZ90_000674"
     tRNA            complement(50443..50516)
                     /locus_tag="IVZ90_000674"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50480..50482),aa:Pro,seq:tgg)
BASE COUNT        14535 a        10737 c         9320 g        15943 t
ORIGIN      
        1 tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg
       61 ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat
      121 tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg
      181 taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt
      241 taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg
      301 tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac
      361 ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa
      421 agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt
      481 acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac
      541 ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg
      601 aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg
      661 tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc
      721 tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga
      781 taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt
      841 aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt
      901 tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac
      961 ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag
     1021 ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc
     1081 ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg
     1141 tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt
     1201 cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag
     1261 caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct
     1321 cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt
     1381 ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa
     1441 gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag
     1501 cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg
     1561 cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat
     1621 ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg
     1681 aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc
     1741 ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt
     1801 gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta
     1861 cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt
     1921 tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag
     1981 aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca
     2041 actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt
     2101 tcttgtgact cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag
     2161 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt
     2221 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa
     2281 cagtttttac gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg
     2341 tttttctgtg gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc
     2401 tccagaaact tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag
     2461 tgtttccaaa cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt
     2521 gttatctgca aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc
     2581 ggatacttca tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa
     2641 tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg
     2701 gcttagatct gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct
     2761 tgcctcctaa cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca
     2821 aaatcattta cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt
     2881 tcagaatcag ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc
     2941 ttttgtgtcg aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt
     3001 actccaactt gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt
     3061 ccgatgcttc tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg
     3121 aaaacaagcg ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca
     3181 aacccgcctg tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc
     3241 cgataaaggt tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt
     3301 tgccgctgta aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca
     3361 ccaagtaaat agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc
     3421 gaaagcagtg catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt
     3481 aaaaatgtat gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact
     3541 acccaatgtt tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc
     3601 tgagtagtaa taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca
     3661 ccaaaagcac ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat
     3721 atatcatttg gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag
     3781 gtggaaggaa agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct
     3841 tctgcccatt ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg
     3901 ctgatcagtt cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca
     3961 gtttttttga ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc
     4021 attggatgaa tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc
     4081 agtttttctg cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt
     4141 gttttctctt catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga
     4201 atgattgtgt ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga
     4261 aagatcaatt cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga
     4321 aaataagcgg atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg
     4381 gttccgatat ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac
     4441 aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt
     4501 tattttatca ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg
     4561 ttaggtcact ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct
     4621 tggtatgaat tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc
     4681 aggtgtcggt tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc
     4741 tggttcttta ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct
     4801 ttgctatttt cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt
     4861 tcttgggcaa ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg
     4921 ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct
     4981 ggcgatactc tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag
     5041 atcttgtttc ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat
     5101 aaaaaaaagt tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc
     5161 cttcatctta ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat
     5221 agtgttctct taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca
     5281 ctaaatggtg ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct
     5341 ctgatatacg gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt
     5401 cgacgaagga aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt
     5461 ttcttgtaat aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt
     5521 tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct
     5581 gcaatatttc gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga
     5641 tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac
     5701 tgatcataag aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt
     5761 gtgcctgttg ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat
     5821 gtcacagaaa tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg
     5881 cctgcgaaaa ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat
     5941 ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg
     6001 atacggcttc tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa
     6061 tcacatattc agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac
     6121 cagccaaagc tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg
     6181 gtacattcaa ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata
     6241 cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga
     6301 ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact
     6361 caagaacagc tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa
     6421 gatatagatt gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt
     6481 ccatttttcc aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt
     6541 gctcgttttc tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt
     6601 cgtaatgata acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga
     6661 ttttcatcga ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc
     6721 tgtgcgatga agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg
     6781 aaggatcgga aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac
     6841 acttccgcaa atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg
     6901 cgcttttcct tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc
     6961 ttgctgtacc cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat
     7021 tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga
     7081 tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac
     7141 tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt
     7201 tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat
     7261 aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac
     7321 tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc
     7381 atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa
     7441 ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag
     7501 tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga
     7561 agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt
     7621 caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag
     7681 cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc
     7741 ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt
     7801 cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata
     7861 ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc
     7921 aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt
     7981 ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct
     8041 agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt
     8101 cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata
     8161 cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt
     8221 tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat
     8281 aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca
     8341 taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact
     8401 ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa
     8461 tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat
     8521 ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata
     8581 tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt
     8641 acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct
     8701 aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca
     8761 ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc
     8821 aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt
     8881 gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg
     8941 tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa
     9001 atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct
     9061 acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc
     9121 atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa
     9181 tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct
     9241 tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc
     9301 ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg
     9361 cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt
     9421 ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct
     9481 ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca
     9541 tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga
     9601 ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca
     9661 atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga
     9721 actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca
     9781 tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct
     9841 gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa
     9901 aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt
     9961 ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt
    10021 ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac
    10081 cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg
    10141 tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat
    10201 tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag
    10261 caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg
    10321 catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg
    10381 taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat
    10441 acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt
    10501 ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta
    10561 cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa
    10621 aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct
    10681 ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt
    10741 cgagggcagc caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat
    10801 cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac
    10861 gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga
    10921 catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta
    10981 tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat tgttattctt
    11041 tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta
    11101 ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac
    11161 catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc
    11221 gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt
    11281 ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc
    11341 gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa
    11401 attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc
    11461 ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt
    11521 gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat
    11581 aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac
    11641 accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc
    11701 tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat
    11761 taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt
    11821 ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct
    11881 cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg
    11941 taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag
    12001 taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg
    12061 taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt
    12121 gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg
    12181 atcccgccat agccgatccc tattaaagaa gcttgtaatg cggcagcact gccactcgtt
    12241 tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct
    12301 gctttcatag gatctagcgt gtaatcggca ccgtgttttt ctgctaattc tctacgctgc
    12361 gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca
    12421 atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct
    12481 tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga
    12541 tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca
    12601 cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac caaactgccc
    12661 gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg
    12721 cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg
    12781 ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa
    12841 attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg
    12901 gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt
    12961 ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata
    13021 cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta
    13081 gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga
    13141 caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac
    13201 agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct
    13261 aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt
    13321 tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt
    13381 ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc
    13441 ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag
    13501 ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa
    13561 ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat
    13621 atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc
    13681 ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc
    13741 taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc
    13801 aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc
    13861 gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg
    13921 cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag
    13981 atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc
    14041 ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg
    14101 actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca
    14161 aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg
    14221 aaaagctgca acctatctac taacagatca ttgacgattc cataactatt attgaacata
    14281 tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa
    14341 cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa
    14401 gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa
    14461 aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag
    14521 ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc
    14581 actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca
    14641 gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta
    14701 cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct
    14761 aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca
    14821 cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact
    14881 tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact
    14941 tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca
    15001 ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct
    15061 tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg
    15121 ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc
    15181 gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc
    15241 atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag
    15301 ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca
    15361 tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt
    15421 gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt
    15481 tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat
    15541 ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt
    15601 tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct
    15661 tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta
    15721 gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg
    15781 atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt
    15841 tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa
    15901 gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa
    15961 gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt
    16021 ttccacctct tattttatta ttttcagcca catatattca tacggctgga atatcaactt
    16081 tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg
    16141 taaaagataa cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt
    16201 cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt
    16261 cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac
    16321 tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg
    16381 ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc
    16441 cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc
    16501 agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag
    16561 atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac
    16621 tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat
    16681 acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt
    16741 ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga
    16801 acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt
    16861 ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa
    16921 taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt
    16981 ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa
    17041 gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa
    17101 aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg
    17161 tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt
    17221 cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt
    17281 caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc
    17341 gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc
    17401 gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg
    17461 caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt
    17521 acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc
    17581 ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt
    17641 ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa
    17701 aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca
    17761 tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa
    17821 atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct
    17881 catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg
    17941 tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac
    18001 tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa
    18061 taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt
    18121 gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct
    18181 agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat
    18241 tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa
    18301 aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg
    18361 aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatataa
    18421 tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga
    18481 cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg
    18541 tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg
    18601 ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg
    18661 cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag
    18721 accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga
    18781 acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt
    18841 gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat
    18901 caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat
    18961 cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag
    19021 aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac
    19081 tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt
    19141 agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa
    19201 actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc
    19261 cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga
    19321 catgtctact ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa
    19381 aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac
    19441 ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc
    19501 gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc
    19561 aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt
    19621 gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat
    19681 tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac
    19741 cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct
    19801 taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata
    19861 agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg
    19921 caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta
    19981 ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca
    20041 tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa
    20101 cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca
    20161 attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt
    20221 ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt
    20281 tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg
    20341 ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac
    20401 gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg
    20461 tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga
    20521 cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt
    20581 ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt
    20641 gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag
    20701 gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga
    20761 tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt
    20821 cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg
    20881 cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt
    20941 ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat
    21001 taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac
    21061 gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt
    21121 tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt
    21181 catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat
    21241 tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac
    21301 cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt
    21361 ctgttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga
    21421 acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa
    21481 agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga
    21541 attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt
    21601 ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg
    21661 aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga
    21721 ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca
    21781 tatcttgaga aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag
    21841 ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca
    21901 tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg
    21961 cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg
    22021 cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt
    22081 cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa
    22141 caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac
    22201 attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac
    22261 gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag
    22321 atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca
    22381 atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat
    22441 tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat
    22501 tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg
    22561 gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg
    22621 cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca
    22681 attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa
    22741 tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat
    22801 attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta
    22861 catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac
    22921 ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa
    22981 tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac
    23041 aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg
    23101 taggaccaaa aatccgctca cagaacaacc cttcacgttc aggttttaag gtacgatagt
    23161 taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag
    23221 ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt
    23281 tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg
    23341 attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt
    23401 ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg
    23461 tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct
    23521 ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca
    23581 atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt
    23641 cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag
    23701 tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac
    23761 caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac
    23821 gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag
    23881 acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc
    23941 tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata
    24001 ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata
    24061 cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg
    24121 ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca acccctttat
    24181 ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga acgatataaa
    24241 cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt
    24301 tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt
    24361 cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta
    24421 ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac
    24481 ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc
    24541 gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat
    24601 ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat
    24661 agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc
    24721 cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat
    24781 gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat
    24841 cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt
    24901 caacgacacc gtcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt
    24961 ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca
    25021 tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg
    25081 tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata
    25141 cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt
    25201 cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt
    25261 agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga
    25321 taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg
    25381 ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa
    25441 cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt
    25501 tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac
    25561 caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca
    25621 atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc
    25681 cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt
    25741 ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag
    25801 caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca
    25861 cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg
    25921 gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa
    25981 tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga
    26041 tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt
    26101 tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg
    26161 gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg
    26221 gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt
    26281 tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga
    26341 tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg
    26401 tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc
    26461 cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat
    26521 ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga
    26581 tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt
    26641 tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt
    26701 ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta
    26761 caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt
    26821 ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg
    26881 gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt
    26941 ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg
    27001 atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg
    27061 gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct
    27121 tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct
    27181 tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt
    27241 cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca
    27301 attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca
    27361 ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat
    27421 aacttttact ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa
    27481 acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga
    27541 caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt
    27601 ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt
    27661 ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg
    27721 ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg
    27781 gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata
    27841 aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg
    27901 attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca
    27961 aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg
    28021 aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag
    28081 tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg
    28141 aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt
    28201 ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa
    28261 aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga
    28321 cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa
    28381 gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg
    28441 cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg
    28501 gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc
    28561 ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag
    28621 gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa
    28681 tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat
    28741 tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt
    28801 tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc
    28861 ttgtcttcta ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac
    28921 aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt
    28981 cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca
    29041 ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt
    29101 tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat
    29161 tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat
    29221 gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct ccttacttgc
    29281 gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc
    29341 ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac
    29401 tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt
    29461 tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc
    29521 gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat
    29581 gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat
    29641 catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt
    29701 ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat
    29761 cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg
    29821 aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta
    29881 gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc
    29941 aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa
    30001 aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta
    30061 ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt
    30121 gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat
    30181 ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg
    30241 atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc
    30301 acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat
    30361 caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc
    30421 ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa
    30481 aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg
    30541 acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa
    30601 aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg
    30661 gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac
    30721 gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta
    30781 caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga
    30841 gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga
    30901 cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac
    30961 aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc
    31021 attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat
    31081 ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc
    31141 agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct
    31201 gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat
    31261 cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg
    31321 ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa
    31381 ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa
    31441 acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg
    31501 caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa
    31561 ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa
    31621 ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa
    31681 acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc
    31741 ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt
    31801 aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca
    31861 cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga
    31921 ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta
    31981 agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat
    32041 tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta
    32101 tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta
    32161 aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt
    32221 tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt
    32281 caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat
    32341 aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac
    32401 ctacttatat gttactacac acccttattt tttacacttt aacagtcacc atcatcaata
    32461 gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata
    32521 tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac
    32581 taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa
    32641 aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg
    32701 ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg
    32761 gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa
    32821 ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt
    32881 ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg
    32941 attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg
    33001 ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc
    33061 ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt
    33121 tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag
    33181 ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg
    33241 atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata
    33301 cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt
    33361 cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag
    33421 aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa
    33481 tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa
    33541 accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg
    33601 atcctaacga aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta
    33661 cagatacatc taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata
    33721 ctacctctac cacaagtgat agcacggata catcagcttc aagcgacagt acagatacta
    33781 cctctaccac aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct
    33841 ctaccacaag tgacagcacg gacacgtcag cttcaagcga cagtacagat actacctcta
    33901 ccacaagtga tagcacggac acatcagctt caagtgacag tacagatact acctctacca
    33961 caagtgatag cacggacacg tcagcttcaa gcgacagtac ggatacatct accacaacaa
    34021 gtgatagcac ggacacgtca gcttcaagtg acagtacaga tactacctct accacaagtg
    34081 atagctcaga ttcatctacc actacaagtg atagcacgga cacgtcagct tcaagtgaca
    34141 gtacagatac atcctctacc acaagtgata gctcagattc atcaacttca agcgatagta
    34201 cagacacgtc gtctaccaca agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa
    34261 gttcggataa caggacgaac tttaacttaa ctaataaaac aagcaatgac aatggaaaca
    34321 aatcaaaaag agcacttcct aaaactggaa gtcaatcgaa taattggata acactcgcag
    34381 gcgttatatt acttgtaatc ggtttaagaa tcacttttat gagttataag aaatcgaaaa
    34441 ggtaaaaaaa tattcaagat aaaaaagagc aacgtcgcta acggacgttg ctctttattt
    34501 ttgcttttta tcataaatac aagaactaca attcaatttg tcaaaaaaca cgtagatttc
    34561 cttctgagaa atttatttac gtatcgaacc tttccctatt cagtctttgc gatccaaaat
    34621 cagttgggta agggaaaaga gattctcttt tgttcgaaga gaggtggcag gtaatttctt
    34681 tatttccttc aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc
    34741 tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt
    34801 cttctctaaa taaggtagta gctttggttt gtttttttgc aaagagtaga tcaacggtaa
    34861 agaatagacc ccttgacgca tgtcttctag tacaggtttg ccgatttcct ggctctcttt
    34921 tgtataatct aaaatatcat cgataatctg aaaggccatc ccgatagaaa gtccgatatc
    34981 tcctgctttt tttgcgaagc gctcgcttgt tccactttca aaagcgccaa cagaacaact
    35041 taattggaac agttcagctg tcttacctga aatattttcc aaatattctt ttacagaaag
    35101 ttcataatta tagcgatcat ccatctgccc taattcgcca ttcaatactt tttccatact
    35161 gcgagaattc aattggagac ttttcaagga agtggagtaa tcagacaata gtttaaaaca
    35221 gcagacaaat aaatagtctc ctgcatagac agaaacgaca ttaccaaact gactacggat
    35281 attaggctga ccacgtcgca gatccgcttc atcaacgata tcatcatgga tcagtgtggc
    35341 cgtatggagc atttcgatag aagctgccaa agcgatggct ttgtctcgat cttgctctgg
    35401 tccaaattgt gagaacaata gctgataggc aggacgcaac agctttccgc cggcatggat
    35461 cgtttctttg atggcttgtt cgacaggttt gtttttcagg cggacagttt cttccatccg
    35521 ttttagtgtt tctgtcaatt ctttacttaa agcaggatag ttttcccaca ttggatgtag
    35581 cttcataaaa aaactccttg tgtgacgatt attttttggt aaacattggg ctccattttt
    35641 gtttggttcc ctgctcataa tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa
    35701 gataattcgc tgcgatcccg attgcaatcc ctgataaaat ccctaaaact gaaaggactg
    35761 gcaagtacaa catgactgtc catgattgtg caatgaaaga agccatcaaa agctgtccga
    35821 cattatgcat gaaaccgccg gccgcactaa ttccgatgat actgacacgt tttggtccta
    35881 attgcttgac caataacatt ccaaaataac taagcaaagc accactcata ctataaagga
    35941 atgtggaaat cgttccgcct agcaaagtag tcaggaatag tcgtagccaa agtagtaaga
    36001 agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt gattagattg gctaagccta
    36061 gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat attttccaat aaaccaatga
    36121 tcactccttg tgcaacaagt agcgaaatat aaatcatttt ttgtaattta ctcatggtgt
    36181 gtgaaggtct aataaatcag actgtcttcg tcacctccat ctgttgaacg gacttcgatc
    36241 actaatttat gtggcaaaca gacgatggtt tcgccatttt tcgttgccca gcctcgacgt
    36301 acgcagatct ggtcatcgca atctgcttct tttatccgta ctcgatcgcc atctatctcg
    36361 atcaagttat aatctccgtg ttcatctttg taagtatatg tgttgctctc ttctccagcc
    36421 atcaaaggga atgttttgat ttctgtgcca tccacacgca gtaccgcttc tttttctgct
    36481 gttgcggtgt tttgctgaag accaaaaatc atgactggta aaaaagaaag aacagtcaaa
    36541 agcaaaataa taaaaacatc ccaaggtttg agcagacttt tttcagcaaa ttctttaagg
    36601 ctcatttttg ttctcctttt tccagattaa ctcacccaat tttagcataa aaacaagagg
    36661 ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac agctttccat
    36721 gttttctaat tctttgggct tattaccgag aagcagcagg tggaacaccg tttataacaa
    36781 gaggttgtga cagaagtggg caacttcaag aaataagaag gaatttacga aaattacttt
    36841 tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc tacttctgga acaccgttta
    36901 taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag gcttatctcg
    36961 ggcatcttat tcctttaatt ttacataaaa aggaggctgt gacggtttgt ctcagcccct
    37021 ttctattgaa atcttgcatt caaataacgt tctttatcta tttatttctt gccgtactgg
    37081 gaacttggtc taccgtacca ccaattacct agtgtacgtt gtagccatgg aattacccag
    37141 cgatccaacc caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac
    37201 caaatgttta cccagtaaaa catgcctgat aagcagaacg tgatcaccaa tacaatcgtt
    37261 gtcgcactgc atagccatgt gaataatcca gcaatcaatg ctaatgccaa ggcaatttca
    37321 aagaatacca tgaatttttg gaagaacatt gctacatcat tgtttggcat gatcacttgc
    37381 atgatctttg tgaaccagtc tggtgacttc tcaatcacag ccattggttc ttcaccgtat
    37441 gcataactca aaccaaaatg tgcagcttga gtcgctgctt ctccggcact tgcccctgct
    37501 ccgctggcag ctgtggttgc atcaccagca gcagaagcac cactggcaac atctgttaca
    37561 ttgtattccc atgtccaagg gaacacactg tttccagtga accatgatcc ttcaccaaac
    37621 caagtacttg gtttgaacac ttcaccattc ccaatgattt ttttggaagc ttcaacgaac
    37681 caaactaaac catagaaaat acgaagtggc acactccata agacattgcc gtaacgagaa
    37741 gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga aaaactcatg cattaaatat
    37801 tggaacatat aataaccaga acgaatatca aagaagtatt tcaaattcac gatatgtttc
    37861 attacgatag ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc taccccccat
    37921 ttggcaccaa tagaaaccat aaatccttga tagtttcctt taaatttatg cttttctccg
    37981 cctttgatcg aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga
    38041 acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa agactaaatc accaatgata
    38101 tagatgtttt tatcttcgta gccttttgcc tgcatatatt cattggcgat caaccgctgt
    38161 ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag ttcctttgac accagctgtc
    38221 caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg atcagcagcc
    38281 acttctacga ttggtgcatt caataataat tccacgttgt gcttacgcaa atagcgttca
    38341 gcttttgcag catcatttcg atccaacata ttcaagatcg tcggcattgc ctctacgacc
    38401 atcagtgtga tttcgtttgg atctaattta taatctttgg ctaggcgatt tttccagtcg
    38461 atcagttccc caatcatttc aataccggta aatccagaac cacagacaac gaaagtcagc
    38521 atggctttac gcagttcatc atctggttcc aatgcagctt ttgctactgt cttctcaata
    38581 tgttcccgaa tccttacaga atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca
    38641 ccaggtgtac caaagtcatt cggctctccg cccattccaa ggatcagctg atcaaaagga
    38701 taggaaccta atttggtctt gacgattttt cgttctttat cgataccgat tacagtgtct
    38761 gtgacaagtt gtacattctt ttgacgagca aacaaatgct gcagatcgta ttggattgct
    38821 tctggttcta cacgtccgcc agctacttca tgcaactcgg tcatcatagt atggtacgaa
    38881 tgacgatcga tcagagtaat tgctacatct tcttttttca gtttctttgc caagtatttt
    38941 gtagctgaaa caccggcata tcctgctcct acaacgacaa tattttgctt tgccattgat
    39001 catccgctcc ttatagacta tctatataat gaatatatga taattctttt agtttgatac
    39061 ttattacaca atctccatat attatataga tgttctgttc ttttgtctaa ctccttttgg
    39121 atttttttca aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt
    39181 gaaatattac atgggttatt tatcaaaaat agttgactta taggctaaaa agattagaat
    39241 gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg tattaaaatg
    39301 aagaaaaaac gttttgtaac tggatttgcc gtattagcat tttcagctct agtgttaggc
    39361 gcttgcggcg cagatgacaa cggaagctca aactcttctt ctgaatcatc tacagcacaa
    39421 tcttcgactg caaaaagcac aacagaatca tctgcaaaag ttgtagctgg cggagatttg
    39481 caagacggca catataaatt agaagaaaaa aattattcaa atggttaccg tgcagtcttt
    39541 gaaatggttg taaaagacgg caaaatcact gaatcaaaat atgataacgt caatgaaaac
    39601 ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa atctggcaca
    39661 aacccagaaa aatacattcc tgagttgaat gaccaattct taaaagcaca aagcgcaagc
    39721 ggtgtggaag tcgtaactgg agctactcac tcatctgaaa gcttccaaaa ctacgcacaa
    39781 caattgatcc aagctgctca agctggtaac acagatacaa tcgaaatcga caatggtgct
    39841 gatttgaaag acggcactta caagcttgaa gaaaagaact actctaacgg ctaccgtgtg
    39901 caattcgaaa tgacagttgc tggcggcaaa gtaactgaat caaactttga ttatatcgac
    39961 aaagacggta aatcaaaaca agatgataca gaatataatg aaaatatgaa agcaaaaagc
    40021 ggtacagaac caaaaactta catccctact ctgaacgacg aattcgtcaa agcaatgggc
    40081 gaagaagatg gctcacctgc taatgtagaa gttgttacag gtgctacaca cagctcacat
    40141 tcattcatca tgtacgcaca acaattagtg aatgcagctg aaaaaggcga cactcaaaca
    40201 atcgaagtag ataatatcgt aacagaaaaa taatcacata atcatactaa taaaagttgg
    40261 agggtgggac gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa
    40321 agatgcggaa aaaactacta ttttccttaa tcacactggg catctctttt atggtgctaa
    40381 ctggctgcag caactcagca aataaaacaa ccactgaaag ttcagcagct accaagatat
    40441 tgaaagatcc atattctgat gaacagttct tgttagggac ttacgttcgt atccgtgtgt
    40501 atgatgaagg caaagaatca gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg
    40561 ataaaatcac gatcaatcaa aaaggttctg agatcgatga agtgaatgag caagctggga
    40621 tcaaaccagt aaaagtttct gatgacgtct acacgttagt caagcgggcc tacgaatata
    40681 gccaagattc tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa
    40741 tcggttttga tgacgccaga aaaccttccc aagaagaaat cgatcaggca ttgaaattag
    40801 tggattatca taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag
    40861 gaatgatcat cgatttaggt gcaatcgcca aaggttacat tacggacgaa gtagtgaaag
    40921 tcttgaaaaa acaaggagtg accactgcca tcgttgactt gggcggtaat gtctatgtct
    40981 taggccacag cccaagaggc gaaaaccaag actggacagt cggcatccaa gatccgaaca
    41041 tggctcgcgg atctgtttta ggttctatta aagaacggaa caaaacatta gttacctctg
    41101 gaatttacga gcgttattta gaagtagatg gcaaaaaata ccatcatttg tttgatccaa
    41161 aaacgggtta tccgttcgac aatgatatcg caagtgtcac gatcataaca gataaatcaa
    41221 ttgatggtga cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa ggactggaat
    41281 acgtcgaaag cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa gacgataaag
    41341 tatatgtgac cgatccaatc aaagacacct tcaaattatc ggaggactcc cattacacga
    41401 tgggtgaccg ttctgaacta aaataaggag tacgaaccat gacactaaaa atctttttag
    41461 aagtagttga aatacgaaca aaggtggcta gtgtatttcc ttttgcggta ggggtattat
    41521 tctctatcgc tttttttcat gagatacatt ggctaaacac ttttttattt tttattggaa
    41581 tgctagtttt tgatttagcg actaccgcta tcaataatta catggattat gaaaaggctc
    41641 attctgaagt atataagtat gaagaaaatg tcattggccg cagaaatcta tctcctgtat
    41701 tgattcgaaa catgatttta gctatgattg catttgtttt tgttatcggc gttattctaa
    41761 cgatcaatac gggttggctt ttacttttga tgggatttat ctgttgtttt attggagtat
    41821 tctatacatt cggaccgatt cctttatctc gaatgccttt aggggaaatt ttcagtgggg
    41881 tgactatggg ccttggaatt tttgccatgg tcgtttatgt caatacattc ggttcagatg
    41941 tctatgatct gacagtcaac ttttcatctg gacaatttga tttggtagga aatctttggg
    42001 ctatagcagc aatcgtatta gcttccttac ccttggtgtt tacgattgcc aatatcatgt
    42061 tggctaataa tttgcgggat cttgaccgtg atattgaaaa tcatcgctat acactagttt
    42121 tttatatcgg acgtcctgcg ggaacagtct tattccagct attgatgtat gcttgctatt
    42181 tcgtgatttt aatggggttg ttgagcggta tctatgaatg gccgatcttg gtcaccttct
    42241 tgaccttgcc aattgtgaag aagcatttgg atcaattcaa gcaaacgtta ccccagccta
    42301 tcagtttcag ctattccatc aaaaatatgg tcttattcaa tgccagttat gtattggggc
    42361 tattactaac aatcattatg caagccattt aacagcatga agaggttgtg aaaaaactgc
    42421 cctgcatcaa gtaataagaa cagccaaata aaaatagctc cttatatttt tcgtttggtt
    42481 gggcttatta ccgcagatgc aagcttttga acaccgttta tcaggaggtt gtgaaaaagt
    42541 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
    42601 atttggctta ataccgagaa actggctttt gaacaccgtt tatcagaggt tgtgaaaaag
    42661 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
    42721 aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc
    42781 agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt
    42841 tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta
    42901 actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa
    42961 cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc
    43021 ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt
    43081 ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt
    43141 catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt
    43201 tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca
    43261 gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat
    43321 attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt
    43381 gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc
    43441 atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat
    43501 caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg
    43561 tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa
    43621 accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag
    43681 ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc
    43741 aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt
    43801 tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt
    43861 ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc
    43921 agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc
    43981 tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt
    44041 gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac
    44101 ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa
    44161 aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta
    44221 ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg
    44281 agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc
    44341 ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc
    44401 aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg
    44461 gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat
    44521 aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat
    44581 cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc
    44641 gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa
    44701 tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa
    44761 ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc
    44821 ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat
    44881 ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga
    44941 aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct
    45001 gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat
    45061 atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt
    45121 aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct
    45181 tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg
    45241 acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc
    45301 cgttttcaat caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga
    45361 attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat
    45421 cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca
    45481 tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga
    45541 taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa
    45601 cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa
    45661 aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac
    45721 aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac
    45781 tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa
    45841 cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag
    45901 taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg
    45961 ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg
    46021 gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga
    46081 aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat
    46141 ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt
    46201 tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc
    46261 caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag
    46321 atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc
    46381 ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt
    46441 cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc
    46501 atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa
    46561 aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg
    46621 acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt
    46681 ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc
    46741 ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt
    46801 tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc
    46861 ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt
    46921 atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag
    46981 ttcgatcata attttctcct cctacaactt cataatagta gcatagctct aaaaactttc
    47041 caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa
    47101 agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt gtcacaacct
    47161 cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac tatagggaac
    47221 ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt gaagcaccta
    47281 ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc aatcgatctt
    47341 caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga tcaaatccag
    47401 ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct tgttctttca
    47461 atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca tctttactca
    47521 atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg aaagctttct
    47581 ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca tcttggacat
    47641 tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa gtcataatca
    47701 tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga ccatcatcca
    47761 atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca tctaagagaa
    47821 tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca tcatacccta
    47881 cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata aattctgaca
    47941 tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt gctttggcca
    48001 attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag
    48061 gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg acagcctcat
    48121 cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt tcgctttctt
    48181 ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta gcaacatctt
    48241 cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca gaaacttttt
    48301 gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt
    48361 gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt ttgatcacag
    48421 ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat tcatcgatca
    48481 aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa agattgactg
    48541 ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat cttgtacgta
    48601 atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga atgcgagcaa
    48661 aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct agggtcgttg
    48721 ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc gaagcatcaa
    48781 ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg aataagatta
    48841 cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca aattcaccgc
    48901 gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc ttttcttgca
    48961 tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca atggctgttt
    49021 tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg ctaagaatct
    49081 ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg ttttcttttg
    49141 ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc
    49201 gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca gaatcaccag
    49261 ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata cctaagttga
    49321 tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa atatgttctg
    49381 ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca aaaacttgtt
    49441 tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata ccaccataac
    49501 caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt tcacgtaata
    49561 cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt tctgaactaa
    49621 ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact gcttgggctc
    49681 tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt aaacgttcca
    49741 gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt
    49801 tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt tggtcttcat
    49861 aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca aataactgat
    49921 ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt cgtatataac
    49981 caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa cgggtattaa
    50041 tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca atccttcgaa
    50101 tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt aaatccgagg
    50161 tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa ttataacgaa
    50221 tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag aatcaacaaa
    50281 aaaaaatacg gaatatacta ttccgtatcg acgcgcccaa gaggagtcga acccctaacc
    50341 ttttgatccg tagtcaaaca ctctatccag ttgagctatg ggcgcatatt caatttaaaa
    50401 aaattataaa tcatttgaaa acaaaaagca agataaaaaa aatcgggaag acaggattcg
    50461 aacctgcgac cccttggtcc caaaccaagt gctctaccaa gctgagctac ttcccgttta
    50521 aaaaataaaa aagac
//
LOCUS       DACDBG010000012        48442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDBG010000012 DACDBG010000000
VERSION     DACDBG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.012"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVZ90_000675"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVZ90_000675"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8261744.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVZ90_000676"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVZ90_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8261745.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVZ90_000677"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVZ90_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8261746.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVZ90_000678"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVZ90_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8261747.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVZ90_000679"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVZ90_000679"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8261748.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVZ90_000680"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVZ90_000680"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8261749.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVZ90_000681"
     CDS             complement(8053..8523)
                     /locus_tag="IVZ90_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8261750.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVZ90_000682"
     CDS             complement(8569..8994)
                     /locus_tag="IVZ90_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8261751.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVZ90_000683"
     CDS             complement(9061..9330)
                     /locus_tag="IVZ90_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8261752.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVZ90_000684"
     CDS             complement(9341..10936)
                     /locus_tag="IVZ90_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8261753.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVZ90_000685"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVZ90_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8261754.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVZ90_000686"
     CDS             complement(14489..15049)
                     /locus_tag="IVZ90_000686"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8261755.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVZ90_000687"
     CDS             15402..16376
                     /locus_tag="IVZ90_000687"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8261756.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVZ90_000688"
     CDS             16546..17946
                     /locus_tag="IVZ90_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8261757.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVZ90_000689"
     CDS             complement(18024..18746)
                     /locus_tag="IVZ90_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8261758.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVZ90_000690"
     CDS             complement(18825..19331)
                     /locus_tag="IVZ90_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8261759.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVZ90_000691"
     CDS             19454..21253
                     /locus_tag="IVZ90_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8261760.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IVZ90_000692"
     CDS             complement(21321..22895)
                     /locus_tag="IVZ90_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261761.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVZ90_000693"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVZ90_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8261762.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVZ90_000694"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVZ90_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8261763.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IVZ90_000695"
     CDS             complement(24672..25310)
                     /locus_tag="IVZ90_000695"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8261764.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IVZ90_000696"
     CDS             complement(25311..25961)
                     /locus_tag="IVZ90_000696"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8261765.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVZ90_000697"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVZ90_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8261766.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVZ90_000698"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVZ90_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8261767.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IVZ90_000699"
     CDS             complement(29104..29844)
                     /locus_tag="IVZ90_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8261768.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVZ90_000700"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVZ90_000700"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8261769.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IVZ90_000701"
     CDS             complement(31215..32153)
                     /locus_tag="IVZ90_000701"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8261770.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVZ90_000702"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVZ90_000702"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8261771.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVZ90_000703"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVZ90_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8261772.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IVZ90_000704"
     CDS             complement(35313..35618)
                     /locus_tag="IVZ90_000704"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8261773.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVZ90_000705"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVZ90_000705"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8261774.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IVZ90_000706"
     CDS             complement(36724..37008)
                     /locus_tag="IVZ90_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261775.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IVZ90_000707"
     CDS             complement(37026..37916)
                     /locus_tag="IVZ90_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8261776.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IVZ90_000708"
     CDS             complement(37936..38154)
                     /locus_tag="IVZ90_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261777.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IVZ90_000709"
     CDS             complement(38124..39824)
                     /locus_tag="IVZ90_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8261778.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IVZ90_000710"
     CDS             40091..40864
                     /locus_tag="IVZ90_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261779.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IVZ90_000711"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IVZ90_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8261780.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IVZ90_000712"
     CDS             complement(41688..42659)
                     /locus_tag="IVZ90_000712"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8261781.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IVZ90_000713"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IVZ90_000713"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8261782.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IVZ90_000714"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IVZ90_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8261783.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IVZ90_000715"
     CDS             complement(45614..46438)
                     /locus_tag="IVZ90_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8261784.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IVZ90_000716"
     CDS             complement(46411..47091)
                     /locus_tag="IVZ90_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8261785.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IVZ90_000717"
     CDS             complement(47114..48052)
                     /locus_tag="IVZ90_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8261786.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT        13285 a        10501 c         8726 g        15930 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaataccca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcggctctat aatttatggg actgatgaat
    48301 cagaattgat tcttcagccc cattttcatt ttaggtatta aaaaacatat cgttctctag
    48361 tatgattaaa tcaccacaaa ataaacaact gagaggacga tacgctatat gaatgattct
    48421 atcaaaaaaa tgctgcgatt aa
//
LOCUS       DACDBG010000013        48070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDBG010000013 DACDBG010000000
VERSION     DACDBG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.013"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..245
                     /locus_tag="IVZ90_000718"
     CDS             <1..245
                     /locus_tag="IVZ90_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261787.1"
                     /translation="YKSASKKLTINIDELADQVLSQLNETQEEYYTMPANDAKDNVSH
                     RFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            471..914
                     /locus_tag="IVZ90_000719"
     CDS             471..914
                     /locus_tag="IVZ90_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8261788.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(920..2104)
                     /locus_tag="IVZ90_000720"
     CDS             complement(920..2104)
                     /locus_tag="IVZ90_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8261789.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2372..3070
                     /locus_tag="IVZ90_000721"
     CDS             2372..3070
                     /locus_tag="IVZ90_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8261790.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            3077..3754
                     /gene="nth"
                     /locus_tag="IVZ90_000722"
     CDS             3077..3754
                     /gene="nth"
                     /locus_tag="IVZ90_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8261791.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3880..4413)
                     /locus_tag="IVZ90_000723"
     CDS             complement(3880..4413)
                     /locus_tag="IVZ90_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261792.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4710..7091)
                     /locus_tag="IVZ90_000724"
     CDS             complement(4710..7091)
                     /locus_tag="IVZ90_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8261793.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(7081..7728)
                     /gene="recU"
                     /locus_tag="IVZ90_000725"
     CDS             complement(7081..7728)
                     /gene="recU"
                     /locus_tag="IVZ90_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8261794.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7792..8331
                     /locus_tag="IVZ90_000726"
     CDS             7792..8331
                     /locus_tag="IVZ90_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8261795.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8421..8840
                     /gene="gpsB"
                     /locus_tag="IVZ90_000727"
     CDS             8421..8840
                     /gene="gpsB"
                     /locus_tag="IVZ90_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8261796.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8862..9229
                     /gene="rnpB"
                     /locus_tag="IVZ90_000728"
     ncRNA           8862..9229
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVZ90_000728"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9438..10604
                     /locus_tag="IVZ90_000729"
     CDS             9438..10604
                     /locus_tag="IVZ90_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8261797.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10650..12146
                     /locus_tag="IVZ90_000730"
     CDS             10650..12146
                     /locus_tag="IVZ90_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8261798.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            12227..12910
                     /locus_tag="IVZ90_000731"
     CDS             12227..12910
                     /locus_tag="IVZ90_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8261799.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13555..14745
                     /locus_tag="IVZ90_000732"
     CDS             13555..14745
                     /locus_tag="IVZ90_000732"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8261800.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14847..15800
                     /locus_tag="IVZ90_000733"
     CDS             14847..15800
                     /locus_tag="IVZ90_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8261801.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15869..17218
                     /locus_tag="IVZ90_000734"
     CDS             15869..17218
                     /locus_tag="IVZ90_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261802.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17401..17724)
                     /locus_tag="IVZ90_000735"
     CDS             complement(17401..17724)
                     /locus_tag="IVZ90_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261803.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17853..19016
                     /locus_tag="IVZ90_000736"
     CDS             17853..19016
                     /locus_tag="IVZ90_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261804.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            19086..20435
                     /locus_tag="IVZ90_000737"
     CDS             19086..20435
                     /locus_tag="IVZ90_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8261805.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20480..20911)
                     /locus_tag="IVZ90_000738"
     CDS             complement(20480..20911)
                     /locus_tag="IVZ90_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261806.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            21299..23941
                     /gene="alaS"
                     /locus_tag="IVZ90_000739"
     CDS             21299..23941
                     /gene="alaS"
                     /locus_tag="IVZ90_000739"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8261807.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPKITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPLYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            24141..24737
                     /locus_tag="IVZ90_000740"
     CDS             24141..24737
                     /locus_tag="IVZ90_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8261808.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24859..26007
                     /locus_tag="IVZ90_000741"
     CDS             24859..26007
                     /locus_tag="IVZ90_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8261809.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(26045..26458)
                     /locus_tag="IVZ90_000742"
     CDS             complement(26045..26458)
                     /locus_tag="IVZ90_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8261810.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26537..26968
                     /locus_tag="IVZ90_000743"
     CDS             26537..26968
                     /locus_tag="IVZ90_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8261811.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(27067..27300)
                     /locus_tag="IVZ90_000744"
     CDS             complement(27067..27300)
                     /locus_tag="IVZ90_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8261812.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27483..29153
                     /locus_tag="IVZ90_000745"
     CDS             27483..29153
                     /locus_tag="IVZ90_000745"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8261813.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            29300..29686
                     /locus_tag="IVZ90_000746"
     CDS             29300..29686
                     /locus_tag="IVZ90_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261814.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29673..30371
                     /locus_tag="IVZ90_000747"
     CDS             29673..30371
                     /locus_tag="IVZ90_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8261815.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30376..30840
                     /locus_tag="IVZ90_000748"
     CDS             30376..30840
                     /locus_tag="IVZ90_000748"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8261816.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30837..31748
                     /locus_tag="IVZ90_000749"
     CDS             30837..31748
                     /locus_tag="IVZ90_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8261817.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31983..32522
                     /gene="pyrR"
                     /locus_tag="IVZ90_000750"
     CDS             31983..32522
                     /gene="pyrR"
                     /locus_tag="IVZ90_000750"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8261818.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32536..33819
                     /locus_tag="IVZ90_000751"
     CDS             32536..33819
                     /locus_tag="IVZ90_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8261819.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33832..34758
                     /locus_tag="IVZ90_000752"
     CDS             33832..34758
                     /locus_tag="IVZ90_000752"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8261820.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34777..36057
                     /locus_tag="IVZ90_000753"
     CDS             34777..36057
                     /locus_tag="IVZ90_000753"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8261821.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            36062..37138
                     /gene="carA"
                     /locus_tag="IVZ90_000754"
     CDS             36062..37138
                     /gene="carA"
                     /locus_tag="IVZ90_000754"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8261822.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            37143..40328
                     /gene="carB"
                     /locus_tag="IVZ90_000755"
     CDS             37143..40328
                     /gene="carB"
                     /locus_tag="IVZ90_000755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8261823.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40382..41158
                     /locus_tag="IVZ90_000756"
     CDS             40382..41158
                     /locus_tag="IVZ90_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8261824.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            41155..42087
                     /locus_tag="IVZ90_000757"
     CDS             41155..42087
                     /locus_tag="IVZ90_000757"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8261825.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            42084..42800
                     /gene="pyrF"
                     /locus_tag="IVZ90_000758"
     CDS             42084..42800
                     /gene="pyrF"
                     /locus_tag="IVZ90_000758"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8261826.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42807..43496
                     /locus_tag="IVZ90_000759"
     CDS             42807..43496
                     /locus_tag="IVZ90_000759"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8261827.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            43469..43738
                     /locus_tag="IVZ90_000760"
     CDS             43469..43738
                     /locus_tag="IVZ90_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261828.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            43776..44417
                     /locus_tag="IVZ90_000761"
     CDS             43776..44417
                     /locus_tag="IVZ90_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8261829.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44448..45152
                     /locus_tag="IVZ90_000762"
     CDS             44448..45152
                     /locus_tag="IVZ90_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8261830.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            45149..46051
                     /locus_tag="IVZ90_000763"
     CDS             45149..46051
                     /locus_tag="IVZ90_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8261831.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(46209..47915)
                     /locus_tag="IVZ90_000764"
     CDS             complement(46209..47915)
                     /locus_tag="IVZ90_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8261832.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15651 a         8417 c        10340 g        13662 t
ORIGIN      
        1 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
       61 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
      121 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
      181 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
      241 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
      301 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
      361 tcaacccctt tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt
      421 tgaataggaa acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa
      481 gacaactgct aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac
      541 cagaaatgga aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata
      601 tcgggaaaat ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag
      661 gagccatcag taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa
      721 gcgaagagaa taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga
      781 atagacacga gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata
      841 tgtcaaaaga agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac
      901 gaagtagcaa atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca
      961 aaaatccgaa taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg
     1021 ggccgatgac gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct
     1081 ggttatctga gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac
     1141 cgaaaccata acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata
     1201 atgtgatgaa tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt
     1261 ctgaaaattt tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc
     1321 caatcaaaga gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa
     1381 attgagaaaa gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca
     1441 ataaaggaat aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat
     1501 gggagaaact taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt
     1561 agccataaac ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc
     1621 ccaactcagc taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag
     1681 cacccatcca tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag
     1741 catataaggt ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca
     1801 tccatatcga atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga
     1861 taataaggat cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata
     1921 agaaggcagc tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta
     1981 tggagtgaga ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta
     2041 atccagtgaa taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt
     2101 acatgtaaaa actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa
     2161 ttttgtttcc ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga
     2221 aaagtgaaga tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa
     2281 tctgaccgat cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt
     2341 acgtatactg tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc
     2401 tggacaaaca gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc
     2461 tgaagaattt ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc
     2521 tgatttgcag atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact
     2581 ggatcgtctc gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa
     2641 agcagatcga tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca
     2701 agaaaaaaaa tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt
     2761 tgaacaagaa tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct
     2821 agaagaagac cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa
     2881 tcaggcttat agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct
     2941 acgtacaaaa caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag
     3001 agaagtagaa cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt
     3061 ggaggagtag cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta
     3121 tggaatgttt cccgaagcac atggagaact caagcacaat aatccatttg agttattgat
     3181 tgcagtgatc ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt
     3241 gtttgcctct tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct
     3301 taaaataaaa acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca
     3361 gcagctgatt gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct
     3421 tcctggtgta ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc
     3481 aatcgcagta gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga
     3541 tgctacggtc atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt
     3601 aaagacacat catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa
     3661 gtgcgaggta tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa
     3721 agaaaaggcg ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa
     3781 tgtatgaatt atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa
     3841 aagtaaatgt acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa
     3901 ataaaagttg ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat
     3961 acctataaaa taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa
     4021 aggtgtaata aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact
     4081 aatggatttg ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca
     4141 taaaaaagtg agaatcattg catgagagct tctttccata aattttcctt taagagtagt
     4201 gcttaaaagt acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt
     4261 taataataaa caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga
     4321 taaaaaaaga tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat
     4381 gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac
     4441 aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat
     4501 tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt
     4561 tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta
     4621 atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa
     4681 ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg
     4741 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
     4801 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
     4861 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
     4921 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
     4981 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
     5041 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
     5101 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
     5161 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
     5221 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
     5281 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
     5341 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     5401 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     5461 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     5521 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     5581 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
     5641 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
     5701 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
     5761 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
     5821 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
     5881 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
     5941 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
     6001 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
     6061 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
     6121 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
     6181 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
     6241 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
     6301 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
     6361 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
     6421 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
     6481 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
     6541 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
     6601 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
     6661 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
     6721 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
     6781 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
     6841 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
     6901 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
     6961 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
     7021 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
     7081 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
     7141 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
     7201 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
     7261 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
     7321 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
     7381 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
     7441 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
     7501 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
     7561 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
     7621 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
     7681 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
     7741 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
     7801 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
     7861 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
     7921 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
     7981 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
     8041 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
     8101 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
     8161 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
     8221 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
     8281 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
     8341 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
     8401 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
     8461 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
     8521 atatcatcaa agattatgaa gcttacagca aagaactcct tgctttacaa gaagaaaacg
     8581 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
     8641 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
     8701 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
     8761 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
     8821 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
     8881 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
     8941 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
     9001 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
     9061 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
     9121 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
     9181 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
     9241 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
     9301 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
     9361 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
     9421 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
     9481 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
     9541 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
     9601 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
     9661 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
     9721 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
     9781 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
     9841 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
     9901 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
     9961 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
    10021 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
    10081 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
    10141 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
    10201 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
    10261 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
    10321 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
    10381 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
    10441 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
    10501 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
    10561 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
    10621 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
    10681 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
    10741 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
    10801 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
    10861 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
    10921 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
    10981 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
    11041 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
    11101 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
    11161 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
    11221 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
    11281 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
    11341 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
    11401 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
    11461 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
    11521 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
    11581 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
    11641 aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
    11701 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
    11761 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
    11821 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
    11881 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
    11941 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
    12001 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
    12061 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
    12121 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
    12181 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
    12241 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
    12301 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
    12361 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
    12421 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
    12481 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
    12541 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
    12601 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
    12661 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
    12721 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
    12781 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
    12841 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
    12901 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
    12961 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
    13021 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
    13081 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
    13141 tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa
    13201 aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa
    13261 taaataaatt aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt
    13321 gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc
    13381 atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa
    13441 ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga
    13501 cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct
    13561 tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta
    13621 tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa
    13681 aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa
    13741 aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc
    13801 aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca
    13861 gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta
    13921 atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt
    13981 tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag
    14041 aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat
    14101 ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa
    14161 aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt
    14221 gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta
    14281 tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg
    14341 ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca
    14401 gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg
    14461 gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct
    14521 gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag
    14581 aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg
    14641 acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca
    14701 gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt
    14761 tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt
    14821 agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac
    14881 aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg
    14941 cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg
    15001 taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc
    15061 atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga
    15121 acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta
    15181 cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata
    15241 ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg
    15301 attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt
    15361 cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag
    15421 caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt
    15481 atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg
    15541 ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat
    15601 ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt
    15661 caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct
    15721 tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa
    15781 aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga
    15841 ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga
    15901 agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt
    15961 tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac
    16021 aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc
    16081 aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata
    16141 tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat
    16201 ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct
    16261 atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct
    16321 gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg
    16381 caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga
    16441 cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc
    16501 gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg
    16561 agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat
    16621 catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt
    16681 cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg
    16741 aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct
    16801 tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct
    16861 ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt
    16921 catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt
    16981 tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga
    17041 acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat
    17101 cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat
    17161 ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct
    17221 aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat
    17281 gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt
    17341 ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt
    17401 ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac
    17461 caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt
    17521 agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag
    17581 attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt
    17641 agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc
    17701 aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt actttacttt
    17761 gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat
    17821 aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt
    17881 ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg
    17941 aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt
    18001 tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg
    18061 cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag
    18121 acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg
    18181 tatttttgga accagctttt aatgttttat tactcatacc atttggcatt tatttaagat
    18241 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
    18301 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
    18361 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
    18421 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
    18481 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
    18541 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
    18601 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
    18661 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
    18721 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
    18781 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
    18841 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
    18901 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
    18961 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
    19021 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
    19081 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
    19141 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
    19201 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
    19261 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
    19321 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
    19381 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
    19441 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
    19501 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
    19561 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
    19621 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
    19681 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
    19741 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
    19801 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
    19861 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
    19921 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
    19981 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
    20041 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
    20101 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
    20161 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
    20221 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
    20281 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
    20341 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
    20401 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
    20461 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
    20521 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
    20581 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
    20641 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
    20701 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
    20761 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
    20821 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
    20881 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
    20941 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
    21001 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga
    21061 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
    21121 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
    21181 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
    21241 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
    21301 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg
    21361 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
    21421 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
    21481 aaaaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
    21541 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
    21601 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
    21661 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
    21721 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
    21781 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
    21841 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
    21901 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
    21961 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
    22021 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
    22081 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
    22141 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
    22201 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
    22261 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
    22321 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
    22381 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
    22441 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
    22501 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
    22561 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
    22621 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
    22681 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
    22741 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttata
    22801 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
    22861 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
    22921 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
    22981 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
    23041 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
    23101 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
    23161 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
    23221 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
    23281 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
    23341 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
    23401 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
    23461 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
    23521 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
    23581 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
    23641 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
    23701 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
    23761 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
    23821 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
    23881 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
    23941 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
    24001 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
    24061 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
    24121 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
    24181 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
    24241 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
    24301 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
    24361 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
    24421 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
    24481 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
    24541 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
    24601 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
    24661 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
    24721 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
    24781 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
    24841 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
    24901 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
    24961 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
    25021 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
    25081 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
    25141 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
    25201 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
    25261 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
    25321 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
    25381 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
    25441 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
    25501 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
    25561 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
    25621 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
    25681 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
    25741 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
    25801 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
    25861 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
    25921 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
    25981 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
    26041 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
    26101 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
    26161 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
    26221 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
    26281 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
    26341 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
    26401 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
    26461 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
    26521 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
    26581 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
    26641 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
    26701 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
    26761 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
    26821 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
    26881 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
    26941 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
    27001 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
    27061 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
    27121 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
    27181 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
    27241 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
    27301 agcactcacg acctttctaa agtctgatca tagccttcat tataaagagt atttataagt
    27361 acgtcaaata aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata
    27421 attggcatat cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc
    27481 aaatgaagaa tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg
    27541 ctagtaaatt agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa
    27601 tcaatttttc tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta
    27661 cttccatcaa tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag
    27721 acgcgctcaa taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc
    27781 ctgtgatggg aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg
    27841 aagatatcaa cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat
    27901 tatctgctct tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc
    27961 gagtgatttg gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg
    28021 gcttgggtgg gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca
    28081 gcgaaatcat ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg
    28141 caaggatcgt tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag
    28201 cagaaggcgc tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa
    28261 tttacggaac accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca
    28321 atagtatttt agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag
    28381 gatttggtgc agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa
    28441 aagcgccgga tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa
    28501 tgaaaaaaga tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt
    28561 taaagcgcca tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg
    28621 aattcgtaac agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg
    28681 ggattcttgc caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag
    28741 caaaagctgt agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc
    28801 tagaagaaac gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg
    28861 gtgttgtttt cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg
    28921 acaaactgcc gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc
    28981 taggtgcacc ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag
    29041 gatttttagt agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg
    29101 ctgcattgaa tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat
    29161 tataatctac catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg
    29221 aattaatttt gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa
    29281 agcataatag gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat
    29341 gggtagctcg attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca
    29401 aaagataacg cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa
    29461 aagccccgtt tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt
    29521 gattcttcaa atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca
    29581 aaatttattt ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa
    29641 aaaccgggca tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca
    29701 gtttcaatcg aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat
    29761 ttaccgaact tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag
    29821 atgatctttt acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg
    29881 attttgtcat tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag
    29941 aactgattca gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac
    30001 aagatcttcc gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc
    30061 cgttatacaa taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc
    30121 tagcaaaaga aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga
    30181 tcatccaaaa cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg
    30241 aggttgaagc gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg
    30301 ggaatcccga tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag
    30361 aaaaggattg attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg
    30421 gatcagttgg tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt
    30481 ataccaggta ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta
    30541 gaagggaaga tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca
    30601 ttgatgatta aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt
    30661 ctagctggtg cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg
    30721 ttccagacag attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt
    30781 ggagtgattt gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga
    30841 cagaacttaa aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt
    30901 tattcaccga ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg
    30961 tcaatggaca gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct
    31021 tgatccccga accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt
    31081 acgaagatga agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag
    31141 caggtcatac gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta
    31201 cgatcaacgg cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac
    31261 tgctaatggt tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata
    31321 agacctcttt gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa
    31381 ccatcaatgc gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag
    31441 atgggaagtc ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg
    31501 ttgaattaaa actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg
    31561 gttatccgtt agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc
    31621 agtttctcca tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct
    31681 ttgaagcacc tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg
    31741 cattttaatt tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt
    31801 cctttaagtt tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc
    31861 ataagtagat tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta
    31921 aacattcctc ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga
    31981 aaatgcaagc aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta
    32041 cttatgagat tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa
    32101 cacgaggaat ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg
    32161 atattcctgt aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag
    32221 aagaaccgga acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt
    32281 tgattgatga tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg
    32341 attttggcag accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat
    32401 tgccaatccg tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt
    32461 tagtagagat gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt
    32521 agaggagaga gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat
    32581 atccacgatc gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg
    32641 atgttcggag caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta
    32701 gtcagttcag gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg
    32761 tacttaggaa gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt
    32821 tatccagcga ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg
    32881 atcatcaaaa agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt
    32941 ccagtcatca tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat
    33001 aatcagggca cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca
    33061 attttcttca acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt
    33121 attagcggat acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac
    33181 gctgcctggt ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta
    33241 tatcttggag caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc
    33301 ggccatctta tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt
    33361 cataagacgt tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct
    33421 cctgtaacaa gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg
    33481 tttgtgatcg gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc
    33541 gctttgattt taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga
    33601 gtaattgccg caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag
    33661 aaaaatctgt tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa
    33721 gtaggcacat ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat
    33781 cttatcttac cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc
    33841 aaatcagaac gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa
    33901 gttatgggct tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa
    33961 aagcgccagt actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc
    34021 ttcgatgtag cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc
    34081 tctgtccaaa aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta
    34141 gacgtagcgg ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa
    34201 acaatccagt gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc
    34261 ttgcttgatt tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc
    34321 atcgtaggtg atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt
    34381 ctaggcgcac aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg
    34441 tacggacatt atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt
    34501 cgggtgcaac acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag
    34561 gaacatgggt taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac
    34621 ccagcaccag ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg
    34681 cggatcgttc agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg
    34741 ctaaatggaa aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt
    34801 cagatcaata cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga
    34861 atcaaagcaa tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag
    34921 ggacaattga tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt
    34981 acatacaagg agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact
    35041 gtatgcgcga tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta
    35101 tatgacatca ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa
    35161 aatctgcgaa gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct
    35221 ttcacaaatg acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa
    35281 gctgcaaaaa acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc
    35341 ggcgttatgc acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta
    35401 accgagtctt ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac
    35461 catgtttgcc atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca
    35521 ggaatccacg taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt
    35581 cccgaagatt tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa
    35641 gctttgatcg aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc
    35701 catggattag aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc
    35761 gaaacagcgt ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa
    35821 caagtgatca attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg
    35881 ctaacggttg gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc
    35941 gataaagaag actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa
    36001 ggcgaaacac aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag
    36061 tatggaacgt ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc
    36121 agatgctagt gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac
    36181 tatcactgat caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa
    36241 ttatggggta aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt
    36301 aaaagaacat gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt
    36361 gaaacaaaaa ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat
    36421 ccgtgaagtc ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc
    36481 atttgatcag ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa
    36541 caaaccttat ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa
    36601 acatagtatt ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa
    36661 cacagatgcc gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc
    36721 tggtgatcca aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt
    36781 accgattttt ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac
    36841 ctataaaatg aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg
    36901 aagaattgat tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc
    36961 agaaaaactc atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca
    37021 cagagattat cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga
    37081 tgcactacat ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac
    37141 agatgccgaa acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca
    37201 ttggtcaagc cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag
    37261 aaggatatga agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag
    37321 tggcagatca agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa
    37381 aagagcgtcc agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa
    37441 tggagctggc agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc
    37501 tttctgcgat cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga
    37561 atcagccaat ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa
    37621 ataaaatcgg ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag
    37681 gtatgtgtga aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc
    37741 ccgctaccca atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag
    37801 tgatgagaga ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg
    37861 tgggtatcca tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg
    37921 aatatcaaat gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag
    37981 gctgtaatgt ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca
    38041 atccgcgtgt ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta
    38101 agctagctgc taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag
    38161 gaacgacttt tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct
    38221 ggccgtttga taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg
    38281 gtgaagtgat ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt
    38341 tagaaatcgg tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc
    38401 taatccaaaa agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta
    38461 gaagagggta caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag
    38521 ataaactgtt gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca
    38581 agatattgaa aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg
    38641 aaataaacga agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata
    38701 agatggttga cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct
    38761 atgaatatga aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg
    38821 gtcctatccg tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag
    38881 caatccagca ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga
    38941 ctgacttttc tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga
    39001 atgtgatcga gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa
    39061 tcaacttagc cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag
    39121 acttggatcg tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc
    39181 ctcaaccttt aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa
    39241 ttggttatcc agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg
    39301 ttgaaaacca gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac
    39361 atccagtact agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg
    39421 acggtgaaac agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt
    39481 caggggattc aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga
    39541 tcgaagatta cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc
    39601 agtttgtcat ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga
    39661 ctgttccatt cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta
    39721 ttttgggaga gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag
    39781 aagttcatgt taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact
    39841 tagggcctga aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag
    39901 ctttgtacaa ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat
    39961 ttacgattgc ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa
    40021 ttggctatag tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg
    40081 tcgttgaaac agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa
    40141 taagagatgg taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt
    40201 ctaaagacgg tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt
    40261 ctttagacac agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc
    40321 ctatttaacg gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca
    40381 gatgaaacaa gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga
    40441 aatgacattg aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat
    40501 tctcgtgccg cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca
    40561 cgaaacaaat acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc
    40621 tactctttct gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt
    40681 gtccaatctt catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc
    40741 tctatatgag ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg
    40801 ttttgccaca catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag
    40861 aatcgctact gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg
    40921 aacgaagacg gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt
    40981 ggaacagctg ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg
    41041 tgggataggg gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa
    41101 aagtgtgaaa gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat
    41161 gagaatcctt tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca
    41221 agcggctgtt tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga
    41281 tcgatcatgg tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg
    41341 gcggaaacac catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt
    41401 gttatgcata aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat
    41461 gttgctggtg cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca
    41521 aatgtgaaag caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct
    41581 tttggaacag attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct
    41641 gttcctgtct atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca
    41701 atcgaagctg gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt
    41761 gaccttaaga caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca
    41821 atcaagcctg tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc
    41881 attgggatgg gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca
    41941 agtgctgtag ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt
    42001 aaagagctcc ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa
    42061 gaagtgagag aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag
    42121 atttttcaac taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat
    42181 tgtttgtgaa aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc
    42241 taaaagaggc tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg
    42301 tcgaaaaagc aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg
    42361 ctggcggtat tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag
    42421 gtggaaaacg tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa
    42481 tgcatgccga tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct
    42541 gtgccaaaaa agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca
    42601 aagaaatggc aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag
    42661 tcgcaggaga tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt
    42721 ttatcgttat aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga
    42781 ttcaaacaga atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat
    42841 tagaaattga agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca
    42901 aaagcccaat ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga
    42961 tcgcaaaagg attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg
    43021 gaactgcaac agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa
    43081 tggtctatat tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta
    43141 tatttgaagg tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct
    43201 tggaagcagc tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct
    43261 ttacgtatga gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga
    43321 cgttgaccaa ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc
    43381 aagaattaac tctgctaaaa gaatggaaaa aagatcctga aaattggcag attgaagcgc
    43441 gcttgatgaa gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa
    43501 aatccaaaaa ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg
    43561 tgatcagttt ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag
    43621 aataatctct tctctttttt ctattatata tagattttct ctattttttg gtaagggagc
    43681 tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata
    43741 ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag
    43801 gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata
    43861 cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag
    43921 aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact
    43981 acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa
    44041 gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc
    44101 cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag
    44161 aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa
    44221 tcatcaaaaa taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga
    44281 ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac
    44341 tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa
    44401 agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata
    44461 tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg
    44521 attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg
    44581 aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg
    44641 acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt
    44701 tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca
    44761 tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga
    44821 atctagttgt gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag
    44881 aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct
    44941 ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg
    45001 ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc
    45061 ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag
    45121 aaatttatta tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc
    45181 tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag
    45241 tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac
    45301 gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat
    45361 attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt
    45421 ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga
    45481 attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga
    45541 agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc
    45601 tggtatcaga agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa
    45661 aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga
    45721 agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt
    45781 ttatgaagac cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg
    45841 tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat
    45901 tggagggatt cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt
    45961 ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga
    46021 gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga
    46081 acaccgttga tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta
    46141 gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca
    46201 ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata
    46261 atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata
    46321 tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag
    46381 gcagctaact cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg
    46441 acgtgcgaac cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc
    46501 attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa
    46561 gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt
    46621 ttcaggtatc cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca
    46681 atttccagtt gagataagac agattcaaga tattggattt catctttggc ttcttggatt
    46741 tgttcgccaa tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt
    46801 gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca
    46861 taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta
    46921 agtagttcgc catttctacg gtaattctca gcgttttcgg attctttgag tgtttgttca
    46981 cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct
    47041 ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct atctaataat
    47101 tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca
    47161 atcggtgtga aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg
    47221 atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt
    47281 ttggtcagct cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt
    47341 ggatctagtt cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg
    47401 ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa
    47461 gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta
    47521 actaagacga tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga
    47581 tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt
    47641 tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc
    47701 ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc
    47761 gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt
    47821 tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg
    47881 accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata
    47941 agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa
    48001 agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa
    48061 atttttaggt
//
LOCUS       DACDBG010000014        47952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDBG010000014 DACDBG010000000
VERSION     DACDBG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.014"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IVZ90_000765"
     CDS             complement(38..1189)
                     /locus_tag="IVZ90_000765"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8261833.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IVZ90_000766"
     CDS             complement(1202..1639)
                     /locus_tag="IVZ90_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8261834.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IVZ90_000767"
     CDS             complement(1654..3420)
                     /locus_tag="IVZ90_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8261835.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IVZ90_000768"
     CDS             3684..4361
                     /locus_tag="IVZ90_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261836.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IVZ90_000769"
     CDS             4364..5491
                     /locus_tag="IVZ90_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261837.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IVZ90_000770"
     CDS             complement(5691..6131)
                     /locus_tag="IVZ90_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8261838.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IVZ90_000771"
     CDS             complement(6142..8175)
                     /locus_tag="IVZ90_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8261839.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IVZ90_000772"
     CDS             complement(8177..9880)
                     /locus_tag="IVZ90_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261840.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IVZ90_000773"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IVZ90_000773"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8261841.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IVZ90_000774"
     CDS             complement(10963..11988)
                     /locus_tag="IVZ90_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8261842.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IVZ90_000775"
     CDS             complement(12197..13066)
                     /locus_tag="IVZ90_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8261843.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IVZ90_000776"
     CDS             complement(13525..15417)
                     /locus_tag="IVZ90_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261844.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IVZ90_000777"
     CDS             complement(15414..17141)
                     /locus_tag="IVZ90_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261845.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IVZ90_000778"
     CDS             complement(17157..17807)
                     /locus_tag="IVZ90_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8261846.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IVZ90_000779"
     CDS             18011..18307
                     /locus_tag="IVZ90_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8261847.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IVZ90_000780"
     CDS             18479..19099
                     /locus_tag="IVZ90_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8261848.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IVZ90_000781"
     CDS             complement(19191..20357)
                     /locus_tag="IVZ90_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8261849.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IVZ90_000782"
     CDS             complement(20369..20722)
                     /locus_tag="IVZ90_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8261850.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IVZ90_000783"
     CDS             20850..21872
                     /locus_tag="IVZ90_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8261851.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IVZ90_000784"
     CDS             complement(22311..22745)
                     /locus_tag="IVZ90_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261852.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IVZ90_000785"
     CDS             complement(22855..24714)
                     /locus_tag="IVZ90_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8261853.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IVZ90_000786"
     CDS             complement(24832..25992)
                     /locus_tag="IVZ90_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8261854.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IVZ90_000787"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IVZ90_000787"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8261855.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IVZ90_000788"
     CDS             complement(27739..28437)
                     /locus_tag="IVZ90_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8261856.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IVZ90_000789"
     CDS             complement(28394..30055)
                     /locus_tag="IVZ90_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8261857.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IVZ90_000790"
     CDS             30273..31673
                     /locus_tag="IVZ90_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8261858.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IVZ90_000791"
     CDS             complement(31925..33004)
                     /locus_tag="IVZ90_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8261859.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IVZ90_000792"
     CDS             complement(33090..34049)
                     /locus_tag="IVZ90_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8261860.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IVZ90_000793"
     CDS             complement(34061..35584)
                     /locus_tag="IVZ90_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8261861.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IVZ90_000794"
     CDS             complement(35594..36157)
                     /locus_tag="IVZ90_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8261862.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IVZ90_000795"
     CDS             complement(36163..37026)
                     /locus_tag="IVZ90_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8261863.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IVZ90_000796"
     CDS             complement(37040..37936)
                     /locus_tag="IVZ90_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8261864.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IVZ90_000797"
     CDS             complement(38111..39382)
                     /locus_tag="IVZ90_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8261865.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IVZ90_000798"
     CDS             complement(39372..40889)
                     /locus_tag="IVZ90_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8261866.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IVZ90_000799"
     CDS             41087..41977
                     /locus_tag="IVZ90_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8261867.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IVZ90_000800"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IVZ90_000800"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8261868.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="IVZ90_000801"
     CDS             complement(42886..44358)
                     /locus_tag="IVZ90_000801"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8261869.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="IVZ90_000802"
     CDS             complement(44995..45357)
                     /locus_tag="IVZ90_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8261870.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="IVZ90_000803"
     CDS             45580..46332
                     /locus_tag="IVZ90_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8261871.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="IVZ90_000804"
     CDS             complement(46472..47395)
                     /locus_tag="IVZ90_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8261872.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="IVZ90_000805"
     CDS             complement(47410..47682)
                     /locus_tag="IVZ90_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261873.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DACDBG010000015        47743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDBG010000015 DACDBG010000000
VERSION     DACDBG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.015"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..2229
                     /gene="acm"
                     /locus_tag="IVZ90_000806"
     CDS             64..2229
                     /gene="acm"
                     /locus_tag="IVZ90_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8261874.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2501..2986)
                     /locus_tag="IVZ90_000807"
     CDS             complement(2501..2986)
                     /locus_tag="IVZ90_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8261875.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3466..4107
                     /locus_tag="IVZ90_000808"
     CDS             3466..4107
                     /locus_tag="IVZ90_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8261876.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4126..4761
                     /locus_tag="IVZ90_000809"
     CDS             4126..4761
                     /locus_tag="IVZ90_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8261877.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4779..5594
                     /locus_tag="IVZ90_000810"
     CDS             4779..5594
                     /locus_tag="IVZ90_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8261878.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5913..7349
                     /locus_tag="IVZ90_000811"
     CDS             5913..7349
                     /locus_tag="IVZ90_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8261879.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7538..9985
                     /locus_tag="IVZ90_000812"
     CDS             7538..9985
                     /locus_tag="IVZ90_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8261880.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10215..11282
                     /gene="rlmN"
                     /locus_tag="IVZ90_000813"
     CDS             10215..11282
                     /gene="rlmN"
                     /locus_tag="IVZ90_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8261881.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11475..12578)
                     /locus_tag="IVZ90_000814"
     CDS             complement(11475..12578)
                     /locus_tag="IVZ90_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8261882.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13131..13679
                     /gene="aac(6')"
                     /locus_tag="IVZ90_000815"
     CDS             13131..13679
                     /gene="aac(6')"
                     /locus_tag="IVZ90_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8261883.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14225..15886
                     /locus_tag="IVZ90_000816"
     CDS             14225..15886
                     /locus_tag="IVZ90_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8261884.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15956..16303)
                     /locus_tag="IVZ90_000817"
     CDS             complement(15956..16303)
                     /locus_tag="IVZ90_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8261885.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16387..17328
                     /locus_tag="IVZ90_000818"
     CDS             16387..17328
                     /locus_tag="IVZ90_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261886.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17329..18372
                     /locus_tag="IVZ90_000819"
     CDS             17329..18372
                     /locus_tag="IVZ90_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8261887.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18378..19448
                     /locus_tag="IVZ90_000820"
     CDS             18378..19448
                     /locus_tag="IVZ90_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261888.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19438..20391
                     /locus_tag="IVZ90_000821"
     CDS             19438..20391
                     /locus_tag="IVZ90_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8261889.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20403..21413
                     /locus_tag="IVZ90_000822"
     CDS             20403..21413
                     /locus_tag="IVZ90_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8261890.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21698..22519)
                     /locus_tag="IVZ90_000823"
     CDS             complement(21698..22519)
                     /locus_tag="IVZ90_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8261891.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22616..22894)
                     /locus_tag="IVZ90_000824"
     CDS             complement(22616..22894)
                     /locus_tag="IVZ90_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261892.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23162..25171
                     /gene="metG"
                     /locus_tag="IVZ90_000825"
     CDS             23162..25171
                     /gene="metG"
                     /locus_tag="IVZ90_000825"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8261893.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25650..26237)
                     /locus_tag="IVZ90_000826"
     CDS             complement(25650..26237)
                     /locus_tag="IVZ90_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261894.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26412..27734
                     /locus_tag="IVZ90_000827"
     CDS             26412..27734
                     /locus_tag="IVZ90_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8261895.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27889..28665
                     /locus_tag="IVZ90_000828"
     CDS             27889..28665
                     /locus_tag="IVZ90_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8261896.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28658..29227
                     /gene="rnmV"
                     /locus_tag="IVZ90_000829"
     CDS             28658..29227
                     /gene="rnmV"
                     /locus_tag="IVZ90_000829"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8261897.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29220..30104
                     /gene="rsmA"
                     /locus_tag="IVZ90_000830"
     CDS             29220..30104
                     /gene="rsmA"
                     /locus_tag="IVZ90_000830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8261898.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30555..31184
                     /locus_tag="IVZ90_000831"
     CDS             30555..31184
                     /locus_tag="IVZ90_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261899.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31308..32417)
                     /gene="ugpC"
                     /locus_tag="IVZ90_000832"
     CDS             complement(31308..32417)
                     /gene="ugpC"
                     /locus_tag="IVZ90_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8261900.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32626..33048
                     /gene="mgsA"
                     /locus_tag="IVZ90_000833"
     CDS             32626..33048
                     /gene="mgsA"
                     /locus_tag="IVZ90_000833"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8261901.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33371..33907
                     /locus_tag="IVZ90_000834"
     CDS             33371..33907
                     /locus_tag="IVZ90_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8261902.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33947..35710)
                     /locus_tag="IVZ90_000835"
     CDS             complement(33947..35710)
                     /locus_tag="IVZ90_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261903.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35713..37428)
                     /locus_tag="IVZ90_000836"
     CDS             complement(35713..37428)
                     /locus_tag="IVZ90_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8261904.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38207..38431)
                     /locus_tag="IVZ90_000837"
     CDS             complement(38207..38431)
                     /locus_tag="IVZ90_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261905.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38631..41399
                     /locus_tag="IVZ90_000838"
     CDS             38631..41399
                     /locus_tag="IVZ90_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261906.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            41480..41899
                     /locus_tag="IVZ90_000839"
     CDS             41480..41899
                     /locus_tag="IVZ90_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8261907.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41911..42381
                     /locus_tag="IVZ90_000840"
     CDS             41911..42381
                     /locus_tag="IVZ90_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8261908.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42398..43177
                     /locus_tag="IVZ90_000841"
     CDS             42398..43177
                     /locus_tag="IVZ90_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8261909.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43164..43985
                     /locus_tag="IVZ90_000842"
     CDS             43164..43985
                     /locus_tag="IVZ90_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8261910.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44002..45051
                     /locus_tag="IVZ90_000843"
     CDS             44002..45051
                     /locus_tag="IVZ90_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8261911.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45064..46068
                     /locus_tag="IVZ90_000844"
     CDS             45064..46068
                     /locus_tag="IVZ90_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8261912.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46701..47720
                     /locus_tag="IVZ90_000845"
     CDS             46701..47720
                     /locus_tag="IVZ90_000845"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8261913.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        15806 a         8305 c         9857 g        13775 t
ORIGIN      
        1 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
       61 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
      121 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
      181 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
      241 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
      301 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
      361 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
      421 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
      481 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
      541 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
      601 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
      661 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
      721 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
      781 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
      841 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
      901 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
      961 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
     1021 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
     1081 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
     1141 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
     1201 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
     1261 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
     1321 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
     1381 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
     1441 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
     1501 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
     1561 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
     1621 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
     1681 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
     1741 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
     1801 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
     1861 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
     1921 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
     1981 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
     2041 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
     2101 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
     2161 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
     2221 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
     2281 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
     2341 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
     2401 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
     2461 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
     2521 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
     2581 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
     2641 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
     2701 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
     2761 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
     2821 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
     2881 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
     2941 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
     3001 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
     3061 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
     3121 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
     3181 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
     3241 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
     3301 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
     3361 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
     3421 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
     3481 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
     3541 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
     3601 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
     3661 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
     3721 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
     3781 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
     3841 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
     3901 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
     3961 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
     4021 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcataat gaaacaagtt
     4081 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
     4141 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
     4201 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
     4261 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
     4321 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
     4381 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
     4441 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
     4501 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
     4561 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
     4621 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
     4681 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
     4741 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
     4801 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
     4861 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
     4921 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
     4981 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
     5041 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
     5101 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
     5161 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
     5221 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
     5281 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
     5341 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
     5401 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
     5461 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
     5521 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
     5581 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
     5641 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
     5701 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
     5761 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
     5821 acctccttta cataatttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
     5881 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
     5941 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
     6001 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
     6061 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
     6121 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
     6181 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
     6241 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
     6301 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
     6361 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
     6421 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
     6481 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
     6541 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
     6601 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
     6661 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
     6721 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
     6781 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
     6841 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
     6901 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
     6961 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
     7021 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
     7081 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
     7141 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
     7201 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
     7261 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
     7321 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
     7381 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
     7441 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
     7501 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
     7561 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
     7621 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
     7681 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
     7741 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
     7801 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
     7861 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
     7921 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
     7981 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
     8041 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
     8101 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
     8161 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
     8221 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
     8281 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
     8341 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
     8401 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
     8461 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
     8521 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
     8581 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
     8641 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
     8701 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
     8761 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
     8821 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
     8881 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
     8941 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
     9001 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
     9061 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
     9121 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
     9181 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
     9241 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
     9301 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
     9361 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
     9421 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
     9481 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
     9541 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
     9601 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
     9661 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
     9721 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
     9781 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
     9841 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
     9901 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
     9961 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    10021 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    10081 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    10141 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    10201 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    10261 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    10321 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    10381 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    10441 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    10501 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    10561 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    10621 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    10681 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    10741 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    10801 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    10861 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    10921 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    10981 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    11041 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    11101 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    11161 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    11221 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    11281 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    11341 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    11401 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    11461 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    11521 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    11581 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    11641 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    11701 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    11761 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    11821 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    11881 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    11941 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    12001 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    12061 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    12121 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    12181 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    12241 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    12301 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    12361 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    12421 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    12481 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    12541 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    12601 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    12661 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    12721 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    12781 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    12841 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    12901 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    12961 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    13021 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    13081 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    13141 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    13201 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    13261 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    13321 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    13381 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    13441 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    13501 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    13561 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    13621 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    13681 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    13741 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    13801 tgtaccagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa
    13861 tggctttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc
    13921 gtttattcag ataagggact gtgacaagac ttttgtcaca gtttcttttt gtttattctt
    13981 ttttatcttt gtatggcaat aatagacaat ataacgaaaa ttatttaatg gcttttggaa
    14041 gaccatggaa aaccttgtac aaagctattt tttctgaaaa attccttgaa acctcacgaa
    14101 aaaatcatta ttttatgaaa aagacaaaaa aaagtgttga ttttttaata attattcatt
    14161 aaactcttaa acaacttcag aaaatattaa ttattctgaa aattccaata aaggggtacg
    14221 taaaatgaaa aagaaattta catttggttt ggttgcggta tgcggccttg tacttgctgg
    14281 atgttatggc ggcagttcag ccacaagcag tagttcagca aaaagcggtt cagcagatgg
    14341 aggaggcgta tttaacttag tcgtgcctca agaaatgcct acagctgact tatcagttgc
    14401 aacagataca atcagtttta cagcattgaa taacgtttat gaaggaattt accgtcttga
    14461 tgaagacagc aaaccacagc cggcaggagc tagcgagttg gcagaagtca gtgaagacgg
    14521 attaacttac aaaatcaaat tacgagaaga cgcaaaatgg tcaaatggtg atcctgttac
    14581 agcagcggat tacgtttatg gctggcaacg aacagtagac ccagctacag cttctgaata
    14641 tgcttacctg tatgcgccag ttgaaaatgc agaagacatt acagctggaa agaaagataa
    14701 atcagaacta gggatcaaag cagttggtga ctatgaatta gaaatcaaat tgacgaaaca
    14761 aacaccatat ttccaatatc tgcttgcttt cccatcattt ttcccacaaa aacaatccgt
    14821 agtggaagaa aatggggatg cctatgcatc tgcaagtgat aaagctgttt ataatggacc
    14881 attcacatta gaagattttg atggaccagg aacagataca gaatggactt ataagaaaaa
    14941 tgatcattac tgggacaaag atacagtaaa actttctgaa atcaaagtca gtgtagtaaa
    15001 agaatcttct actgcattga acttattcaa agatggccaa gcagatgacg tgatcttgtc
    15061 tggtgagtta gcacagcaaa acgcaaacga tccagcttat acttctgtta aagaagcgcg
    15121 gacaagctat atcgaattca atcaacgtga aaagaattca ccattcaaca atgtgaacct
    15181 aagaaaagct atttcttatt ctatcgatcg tgaagcttta gtaaaacaag tactaggtga
    15241 cggatcagtt gtctctacag gattgatccc atcagatatg gtaaaaagtc caacaacgaa
    15301 cgaagacttt gcaaaagaag ctggaaaatt agtttcttat gacaaagata aagcaaaaga
    15361 atattgggaa aaagcgaaaa aagaattggg cgttgattct ttagaatttg atttgatggc
    15421 ttctgatgat gattcatcga aaaaagtaat cgaatatgtt caaaactcaa tccaagaaaa
    15481 cttggatggc gtaacagtca aaccaacacc tgttccattc tctgtacgtc ttgaccgctc
    15541 aacttcgggt gattttgacg cagtactagg cggatgggct gctgactatg cagacccaag
    15601 cagctttact gatttgtttg tcacaggtaa ttcatacaac cgtggtcgct ggagcaatgc
    15661 agaatatgac gaagcagtta aagcagcttc atcaacagac gctggcgatg aacaagcccg
    15721 ttgggatgat ttccaaaaag caatcaagat catctctgat gatatgggtg tcgttccggt
    15781 gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc aaaggtatcg ttcaccatgc
    15841 ggcaggcgct tcatgggatt acaaatggac atacgttgaa gaataatcgt taataccgtg
    15901 agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct cgttctcata ttcacttatc
    15961 ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat tagtattccg gcaatgatca
    16021 gccaaaagct gtacataccg tagctggcag acgagagcga ggaaaattct ggttttttct
    16081 ttcggttcct tgttcgtgca agatggaccg atcgctgaaa atgatcgaag aaacccgaag
    16141 aaaagaccca taaaaaaccg ccgataatca gaaacggcaa tgaccataag aatagatcat
    16201 tagataattg aatgagtgta aaggaatggt tgatgagatt ttttattaaa ataaccagaa
    16261 tcagtagaaa agctactgcg taggggatag attttttttt catttgattg ctcctttatt
    16321 tttggttcat tatctaataa tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa
    16381 acattagtga atagttatat caaatatgta ttaaaacgtg tctttttcat gatcatcacg
    16441 ctttggttaa ttgcaacaat cacctttttc ttgatgcaat tgctgccggg cacgccatat
    16501 acgaatcagg aacgtttgag tcctgaaaca atcgagatgc taaataaaca agtcggtttg
    16561 gataaaccag ttatcgtaca atatgggatt tatctttcta atttgcttca aggagatttt
    16621 ggaatttcct tccaatttaa aaaccagcca gtcgcacatt tgttagctgg aagaatcgga
    16681 ccatccttgc agttagggtt acaggcaatt atctttggaa cagtcctagg gacgatttta
    16741 ggaacgatct ctgccatgaa gcaaaataca tgggcagata catcttctac actcgtagcg
    16801 atattaggtc gttccattcc aaactttgta tttgctgttt tattacaata tatctttgca
    16861 atcaaattac aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac aatcttacca
    16921 acaattgcgt tggccatgtc gccgcttgct gactctgctc ggtttatccg tacggaaatg
    16981 gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg
    17041 gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc cattgatgac tttattagga
    17101 ccgttagctg tagcattgat gaccggatca ctggtggtag aaaatatctt tgctattcct
    17161 ggtatcgggg aacaattcgt taagtcaatc acaacaaatg attatcctac gatcatggct
    17221 gtaacgatcc tttactcatt tatgctgatt tttgttattt tgatcgtgga tctattgtac
    17281 ggtctagttg atccaagaat tcgtgtttca gaagggagcc gaggataaat ggaaatgata
    17341 ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg aatttcttcc attacaaaaa
    17401 aataccgaag aagaacgtga acagattact gcaccttctt tgaattttat ccaagattca
    17461 tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa tggttttact attggtcatc
    17521 attttgatct ctgtgatcac aatctttgtt tctcctcatg atccaactgc ccaaaacgtg
    17581 gcttatatca atttgcctcc tcgtattccg ggaatcaata tcgatggatt gaatggaaaa
    17641 gcaatggttg gtggtgaact tgttgataaa tatgctcaag caaacgtccc ttcaaacgta
    17701 aatttctttt taggaacaga cggtttggga cgtgatgtat taagccgctt atttatggga
    17761 atgagaatct cgttgctgat cgcatttatt gctgcattac tagatgtaac gattggtgta
    17821 gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt
    17881 ttggaaatct tatcaggaat tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt
    17941 gaaccaggaa ttttttcaat cgtggctgct atggccatca caaactggat accaatggca
    18001 cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag aatatgtttt agctggattg
    18061 acattaggtg aatcaagaat gaaaattgca ttcaaacata ttttgccgaa tatctctagt
    18121 gtcattatta ttcaaatgat gtttagtatt ccatctgcta ttttcttcga agccttttta
    18181 agctttatcg gtttaggtct tacgcctccg tcagcttcat taggaacgat gttgagcgaa
    18241 gggtacaaga cattcctgta tctgccttat ctattatgga ttccggcagt aactttatcg
    18301 gttatcatga ttggctttaa cttacttgct gatggattgc gtgatgcctt tgatcctaaa
    18361 atgaaagagt gaagacgatg acgaaaattt tagaagtgaa agacttagaa atctcttttg
    18421 atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg aataaaggag
    18481 aaacattggc gatcgttggg gaatctggaa gtggaaaatc agtgacgacc agaagcatca
    18541 tgcgcttgct gtcaagcaac gccaatatag atgctggtca aatcctcttt aaaggtaacg
    18601 atatcgttgg aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa atagcgatga
    18661 ttttccaaga tccaatgacc tctttagatc cgacgatgcc cattgggaag caagtcgctg
    18721 agtcccttag aaagcataat aaagtttcaa aaaaagaggg acaaaaagct gcattagaat
    18781 tgcttaaatt agttggaata cccgaagctg agaaacgtat caacagttat ccgcatcagt
    18841 tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt tatccggaaa
    18901 tcctgattgc tgatgaacct acaacagcgc ttgatgtgac tattcaagca cagattttgg
    18961 agctgctgaa agaaattcag acaaaaatcg atacatcaat tatctttatc actcatgacc
    19021 ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga agaatcgttg
    19081 aagttggaac atcggaagaa attttctaca acccgcaaca tccctatact tggggactgc
    19141 ttggttcaat gccgacatta gatagtgcga acgatcgatt gtatgctatt cctggttctc
    19201 caccggattt attgaacccg ccaaaaggag acgcattcta tccaagaaat gaattcgcga
    19261 tgaaaatcga tgcagaacaa gaaccaccat tttttgaact atcaaaaaca cacaaggcag
    19321 caacttggct acttgctccg caggcaccca aagtgacacc gccagaagaa atccaacgtc
    19381 gctgggctat ctttaaagag aaacaaaaac atacttacgg tcaaggagga attgctgatg
    19441 ccaaataaac tacttgaagt taaaggattg aaacagtatt tcaatgtagg aaaaaagaat
    19501 gaagtacatg ctgtaaatga catcagcttt cacatttatg aaggagaaac attcggttta
    19561 gttggagaat ctggaagcgg aaaatccact actggccgta cgatcattcg cttgaatgaa
    19621 cctactgatg gtgagatatt attcgacggt caagatataa cgaagatcaa agataaaaaa
    19681 ggtttgacga aattccggca tgatgttcag atgatctttc aagatccata cgcttcgttg
    19741 aatcctcgta tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa tggactagct
    19801 aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt tggattgaat
    19861 ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt
    19921 attgcacgag cattggcagt aaaaccgcgt tttatcattt gtgatgaacc aatttctgca
    19981 ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc aagatctcca aaaagaacat
    20041 caattgacct atctatttat tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga
    20101 atcggtgtaa tgcataacgg cttgttatta gagatgggaa caagtgatga aatctataat
    20161 cacggtgttc atccttatac agaaagtttg ttatctgcta ttcctctacc agatccagac
    20221 catgagagaa aacgcagaag aatcaaatat caacctgaac cagatgacgg acaagtacgt
    20281 caacttcgtg aaatcgcacc agaacatttt gtctatgcaa ctgagcagga agtcccatat
    20341 tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg ttgctaacta ggagggacat
    20401 agatgctaaa agcttttaaa ttccgtatgt atccgacaga agaacaaaaa cagcaattga
    20461 ttcgtacttt tggatgtgct cgatttacct ataaccattt gctgaaaaag cggcagaaaa
    20521 gttggcaaca aacaggagta gccaattttt cgctcacacc cgctacatta aaaaaagaat
    20581 acccattctt aaaagaagta gacagtcttg ctttagcaaa tgcacagctt aatttggatc
    20641 gggcttttcg caattatttc aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa
    20701 gtatgtggca atcttatacg accaacaacc aaacccgtac gatctatctg gaaaatggct
    20761 acttaaagtt accaaaacaa aaagaactaa tcaagataaa tcagcatcgg ccagttgaag
    20821 gatcgattcg ctccgctacg atttctgctc gatataatga agaattttat gtcgcactac
    20881 tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa atggatcggg atcgcctatc
    20941 atccgaagac attgatccaa acatctcgac ctctagaagt aactttacca aaatttcacc
    21001 agacagaaga aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc
    21061 accatagaaa gatcaggtta gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa
    21121 tggatcttta tctgaaacaa aagaaccagc gagaagatta tcttgaacaa ttatctggga
    21181 aattggtcaa acaatatgac tacttatttg tagaatcttt tccaaaagaa gaggcacatg
    21241 ctgatttttc tattcacgac tggcataaat taataacaaa attgcgttat aagtcccaat
    21301 ggtataataa aaaatttctt ttgatcaata ccgacggagc agaagaaagc aattccgtaa
    21361 gaaagagcca agtagtagaa aaaatgggca gacattcggt tatcaaggga taaaaaatga
    21421 atgaaactga taggagcgtt agggttagcc ttgttaataa aaagtcttaa tgacttttgt
    21481 ttctaggaat ttttcttttg aagaaaagct cactaattat gacaaaccaa taaaacaata
    21541 gcagaaaaag ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac
    21601 gaatacatca aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt
    21661 tcttgaatga ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga
    21721 gctgatagat ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat
    21781 attggagcat agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga
    21841 atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa
    21901 cttgcggctc gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac
    21961 tatggtcaaa agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga
    22021 tagccacttc tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc
    22081 gtacttcctt ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc
    22141 tgactgtgac gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct
    22201 caatattttc ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt
    22261 ctccagttga ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag
    22321 gatgcccttt tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca
    22381 tcagtacgat atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat
    22441 accagttaag aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt
    22501 tagaagcaaa acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat
    22561 tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa
    22621 tatgaaaaat aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata
    22681 ctttataccg ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga
    22741 taaaacgcaa aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat
    22801 aaccatacat acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa
    22861 tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata
    22921 gatagtcttt attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat
    22981 gaaacaaaac aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa
    23041 cgtagataga agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt
    23101 tttttatggt atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc
    23161 cgtggcagaa aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact
    23221 tcatatcggc aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt
    23281 gatgggcttt gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac
    23341 aaaagcagaa gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt
    23401 caagaaatta tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga
    23461 ttaccataaa gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta
    23521 cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca
    23581 acttgcagaa gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg
    23641 acacgaagta gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga
    23701 ccgtctattg gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa
    23761 cgaaatgatc aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac
    23821 ttttacttgg ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga
    23881 tgcattatcc aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga
    23941 aaaatactgg cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat
    24001 ctattggcca atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca
    24061 tggctggcta ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc
    24121 tgaaatgctt gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt
    24181 tccgtttggg tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga
    24241 tctggcaaat gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg
    24301 tgatggactc gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac
    24361 gacggctgct aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac
    24421 agcattggct gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga
    24481 accatgggta ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca
    24541 tttagctgaa tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc
    24601 agagaagatc ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca
    24661 ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg
    24721 tttagatatg gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac
    24781 aaacgaagat acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa
    24841 acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg
    24901 tcaaaaagta gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca
    24961 agaccgtcaa atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg
    25021 gaaaaaagtc gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca
    25081 aggaatgatc ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa
    25141 atccatgcca aatggctcag aaatcgcata gaaattcaaa gggggtatga cactagttgt
    25201 gtcatacccc ctttttccga aatttgagaa gcaattttcg taaattcctt cttattactt
    25261 gaagccaatc atataataca gaaacagaga actatccaaa ataaatccta gaaaaagaag
    25321 tacgagcttc gctgattttt gttaatttag aataatacta ataggaatcg taaaaacaga
    25381 aaaaaagcat aatgataaag ggtcgggcag attttaattg attcatctgg tactcttttt
    25441 tattgtcctt acattcataa agtttgaatg caatgacttt attacatcta tatttatatc
    25501 taacagtagc gttgatgtgt taattataag ttctatcact ttatgttcaa ttagtttgtg
    25561 ctcttatatg tgtgattgaa aatatagata aaaaaacaat catcggcttt gacttcgatg
    25621 attgttttct ttattttgta aaaatcctat cacttgattt tatggtaacc ttttattttt
    25681 gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa atgggatcga aaagacatac
    25741 cagaaaatcg tacgtccgat accataatgt tgatagagct ttgtcaacat cacgtgtaac
    25801 aagatcaacc ctaaaagcaa ggagccagct ataaaccaat gattatccca atgcatgact
    25861 tcactaagtg ccatcactcc aaaaggatag aaataaaaat agatcccgac taaggtcatc
    25921 cgccagttct ttgtaaatgt tccgatattc aataaaaaga aaatcgctga aagcaaaata
    25981 ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa gatcatcaca
    26041 ggacctttga tcaatgcaac gattgcgatg aacaaagtca gcaatattag tttttgtgat
    26101 aacgttgcaa cagtcattaa tccaaataaa attaatgtga accaatgcca atgggactgc
    26161 tgttgttctt tacgggaaaa atcgacccgg ttggataaat aatctgctaa tttcggataa
    26221 gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa
    26281 aaataaaaaa gaaatacatc cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa
    26341 gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta taccaaattt caaagaagaa
    26401 agaggatggg aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc gcattagcta
    26461 ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct gttactgcta
    26521 gtactcagtc tacaagtttg acattgctag cttggctttt gggtggtttc ttaacgatct
    26581 gtgctggatt gacagtggct gaattagcta cagcaatccc ggaaactggt ggagctgtaa
    26641 aatatattga agctgcgtat ggaaaattgc ccagcttttt attaggatgg gcgcaaagct
    26701 tgatttactt tcctgctaat attgctgcat tatcgattat ctttgcgaca caattgacga
    26761 acttattaca attatctacc gattatcttt tgttgattgc agtgatcacc gctgtttctg
    26821 tgacagggtt gaatttgtta gggacaaaag tcggtgcttc tgtacaatca gtgacgttga
    26881 ttgttaaatt aataccaatt gctgtgattg tcatatgggg attattgacc ccaggacaag
    26941 gaactgtcca gttatttcca atcgaagctg gaaaagatgt tacttttgtc gaaggattga
    27001 gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg
    27061 caggggaaat gaaacggccg gaaaaagatt tgcctaaagc aattattctt ggtttgagtt
    27121 ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt gaaaactttg ccgattgatc
    27181 atcttgccgg taatttgaat gcagcttctg aagcatcaga cgtcattttt ggaggaatcg
    27241 gtggaaagct ggtcactatt gggatcttga tctcggtata cggtgcattg aacggttata
    27301 cactaactgg gatccgcgtg ccttatgcga tggctttaga agacgagcta cctttcagta
    27361 aacagctgac aaagctttca aagaagttta cggttcctta tgttcctgcg gttttccagt
    27421 tagcagtagc ttgtattatg atgagccttg gttctttcga ctttttaaca gacatgttga
    27481 tttttgtgat gtggctattc agtttactga tttttatcgg tgtctttatt ttaagaaaaa
    27541 aagcgccgga attgccacgt ccatataaag taccacttta tccaatcgtt ccgattattg
    27601 cgattctcgg tgctattttc attttaggta tgacgatgat gacgcaaacg aaattagcat
    27661 tgatcggtat aggtgttacc ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac
    27721 gctcagaaga gtaaaatatt tcaacaacag acagaaaaag tacgaactag gtcctctaga
    27781 tcttcgtgct ttttctattt caataaaaca agttcttcta ttttgatttt tgctacttct
    27841 gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag agataaacat gatatttgat
    27901 tctcataccc atttaaatgc tgaacagttc aatgaagata ttccagaaac gattcagcga
    27961 gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa
    28021 aagtctttgc aattaagcca tgactatcca aatatataca gtattatcgg ctggcatccg
    28081 acagaggctg gcagttatac gaaagaaatc gaaaataaat tacaagaaca attgacttta
    28141 ccaaaggttg tagcattagg ggaaattggc ttggattatc attggatgga agacccgaaa
    28201 gaagtccaag ataaagtatt tcgcagacag attgctatcg caaaagaaat gaacctacca
    28261 atcagtattc atactagaga agcacttgaa gacacgtatc aaatcctaaa agaagaagat
    28321 gttcgagata tcggtggaat catgcatagt ttcagtggtg attatgaatg ggcaaaacga
    28381 tttcttgact tagggatgca tatttcattt agcggtgtcg tcactttcaa aaaagcatta
    28441 gacgtccaag aggctgcagc aaatgtacca atcgaacgat tacttgtgga aacagatgcc
    28501 ccttatctgg caccagttcc ttttagagga aagcgaaatg aaccaggcta tactcgttat
    28561 acagtagaaa agattgctga acttcggaat cttccgacag aagaagtggc tgcccaaaca
    28621 tggacaaatg cgcaccgatt atttggtcta agcgaacatg gataagatga agatccaaga
    28681 gattatagtc gttgaaggaa aagacgatac aagaaggctg cgggaagtag ttgatgcaga
    28741 tacgatagaa acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc
    28801 ccaagagact cgtggagtga tcgtttttac cgatcctgat ttttcaggag aaaaaataag
    28861 aaaaatcatt atggaagctg ttcctcaggc aaaacatgct tttttgccgc gcacacaagc
    28921 taaaccaaag aaaaagggaa gccttggtgt cgagcatgca agtgatgctg ctattttaga
    28981 agcattgaaa aaggtcgtga cgccagacat ttcccaaacg gctgtttctg aaatcactag
    29041 acaagacctt ttagactatg gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa
    29101 actaggtgac gagttgcgca tcggttacac gaacggcaaa caattggaaa aacgtttgaa
    29161 gatgtttcgt attacgccga aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca
    29221 tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa agagatttta ttgaaacatg
    29281 gtttttcatt taaaaaaagt ttaggacaaa attttctgac tgaaccaaat attttaagga
    29341 aaatcgtaga aactgcgcaa atagatgaac atacgaacgt gatcgaagtt ggaccaggaa
    29401 tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca agtcgtagca tttgagatcg
    29461 atgatcgttt gatccctgta ttggctgata cgatgcaacc ctatgacaat gtgactattg
    29521 tacatcaaga tattttaaag accgatttgt caacagctgt tagagaaaca tttcatgagg
    29581 agcttccgtt aaaagtcgta gcgaatttac cttactatat tacgacaccg atcatgatgc
    29641 attttcttga atcagatttg gttgtggatg aactagtcgt catgatgcaa aaagaagtag
    29701 ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg ttcattatcg attgctgttc
    29761 aatattatat ggaagccagt ttagcattca tcgtaccaaa aacagtattt gtccctcagc
    29821 caaatgtaga ttcagcgatt ttgaaactga caagaagaga tataccggca gtcgaagtga
    29881 cagacgaaaa agcatttttc cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc
    29941 tttggaataa cttgcagcat tcgtatggca aagatgacca gacaaaagca tggttagcaa
    30001 aaagcttgga aacagctgga atcgatccaa aacgtcgtgg ggagactttg tcattacaag
    30061 aattcgccgc attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac
    30121 agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
    30181 ctcatgtttt tcgtttgttt gggcttgtta ccgcagatgc aagcttttga acaccgttta
    30241 agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca
    30301 gcttctcatg tttttgagtt agttggtctt attacttgat gcaagctttt gaccactatt
    30361 atgccgaaca aggagatgtg acacaaattt tgtcacatct cctatttttg tttcttgatc
    30421 aaaaaattct tatacttgat actcacactc attttgtatg gatttagaaa agaatatgtg
    30481 tcttgacagt tattagtaag tgagttatac taagtcgaca tttatgtgtc aagacatatt
    30541 aggagagaga aaaaatgaga aaaaattcag ttcgaaaact aacgatttct gctttattga
    30601 ttgggatggg ggtcattatc ccaatggtca tgcctaaaat catgattcca ccagcttcct
    30661 ttactttggc gtctcatgtg ccattattta ttgcgatgtt ttttacacca ggagttgcag
    30721 tggctgttgc gctaggtacg acatttggtt tctttttaac aacgccggtt atcatcgcat
    30781 tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc ttggtacttg caaaagcatc
    30841 cgagtatcgt tttaaaaaat ggacagttta cttttgctaa ttggcgtttc caagggttca
    30901 attttgtgat cggtgtgatt cattctttag cagaaatgct agtagtcagc atcttttact
    30961 ttattggtaa tatgcctgaa acatattatt ctcaaggata tttttataca gtattcatct
    31021 tgatggggat tggtgggctt atccacagtt tagttgatta taatattgct tattttatag
    31081 caggaacact cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc
    31141 ggaaagagtc gattcaaggg tacgcgaaga aaacgacata ttagacatga tctctcaaaa
    31201 aaaaaactct catctagtaa tggataataa atcctctaag atgagagttt tttgtcctaa
    31261 aaagagctgc accaacatct tctggcacag ctctaacagg gggcaattta tggaagtttg
    31321 atgacttgtt ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg
    31381 actatttcgc ctggtttatg gaaatctctt gcatctactt ttgagatgaa ctcagtagaa
    31441 ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac aacttctgat
    31501 ttcactacgg cttcaggtga agcttcaagg gctacttgtt cactgtggat gtcttctgga
    31561 cgaataccga agataacttc ttttccttca taaccttttt caactaatac tttgtttttt
    31621 ccttcaggaa tacgaagctt aagcccatgt ccatctgtga taacaccatt tttcaatgtc
    31681 acattaaaga agttcatggc aggagatcca ataaagccgg caacgaacat attgacgggt
    31741 gtatcataaa cttctttcgg tgtaccgatt tgttggataa atccgtcttt catgataacg
    31801 attcggtcag ccatagtcat tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt
    31861 tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa tttagcatct
    31921 aaatttgata atggctcatc cattaagaag acttttgcat cccgaacgat tgcccgtccc
    31981 aatgccacac gttggcgttg tccaccagat aaagcagctg gttttcgttt caagtattct
    32041 gtcagaccta agatctctgc tgcattgtcc acacgttttt tgatgtctgc tttatcatat
    32101 ttacgtaatt tcaagccaaa agccatatta tcaaaaacag tcatatgagg atacaatgcg
    32161 tagttttgga aaaccatggc aatatcacgg tctttaggtg ccacgtcatt catgactttt
    32221 ccgccgatat ttagttctcc ttcagagata tcttccagtc ccgcgatcat tcgcaaagta
    32281 gttgactttc cacaaccgga aggaccaaca aaaacgataa attcacggtc tttgattgat
    32341 agattaaaat cagttacaga ataattttct gcattgtcat attttttata tacgtttttc
    32401 agagccattt ctaccataat acgttccaca ctttcttttt tagttttatt aactatttaa
    32461 agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt
    32521 tttgtcaatg tgctagtttt aagctttaat aaatatccat aagaaataaa aactgataca
    32581 ctggagtaaa taagtatgtt aacaatatca aaaggagtgt ctcttatgaa aatcgctttg
    32641 atcgctcatg accgaaaaaa acctatgatg gtgaaattag caacagcgta taaaacaata
    32701 ttggaaaaac atgaactttt tgctaccggg acgactgggc agaggatcac ggaagcaacc
    32761 ggcttgccga tccatcggtt taaatccgga ccgttaggcg gggatcagca aattggagcc
    32821 atgatttcgg aggatcagtt agatttggtg atttttcttc gagacccgct ggctgcacag
    32881 ccccatgaac cagatgtaac agcacttatc cgtttatctg atgtctatga gattccgtta
    32941 gctacgaata tcggaacagc tgaggtgcta ctaagaggac tggaagccgg cttcctagat
    33001 tttcgaagaa tcgtccatga aatagacaag catccacttt cattttgatg gataagcgta
    33061 tggaaaaata acgtttaatg ttcgcctata tgtagctata aaagctaaac aaactgataa
    33121 tatgcgatat aatacgaacg tattggataa tataaacaat aatattagtt tttcgtattt
    33181 gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga
    33241 gtagaaaata aagcgttaag tgctaagagg atgggatgtt gcctcttgga cgaaggacgt
    33301 aatgttcgcg gttttatttt cgcattcgct gcatgatacg atgtagcagc tcttaaagga
    33361 gatgctaaaa atgaagaaac accggttaga cgcacgtatg attgctatta tgggcttatt
    33421 gattgcattg atggtcactt tgtcccgact ggtagcaatc gagaccccat ttattaaaat
    33481 cagtgtcaca tttattccac aagtgatcat gggcattctt tttggtcctt tttggtcagg
    33541 gataggcgca gttttagctg atttagtagg aatggccctc ttctcgaaat ctgcattctt
    33601 tattggattt acattgaatg catttattga aggagcaatc tacggattct ttttctatcg
    33661 aaaagagatc acttggaaaa atgccattct ggctacgctt agtgtcacgc tgatcatcaa
    33721 cttatttttg acaccattat ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt
    33781 cgtttgggcg ccccgcctgt taaaaacagt aatatggtta ccgattcaat caattgccat
    33841 ttattacgta ggacgtagta ttccttacaa aaaaatttta agaagtttag cgatccacgc
    33901 taaataaaaa acaagcagtc ttcccatccc agaagactgc ttgtttttat tgtgctgtga
    33961 attgtgcttg atagagccgt cggtaataac catctttttg aagcagctca tcatgggtgc
    34021 ccatttcgac aatagctccc tgatccatca ctaatatctg gtccgcattt ttgattgtgg
    34081 acaggcgatg ggcaatcaca aaactggttt ttccttccat cattgctaag aaggcttgtt
    34141 ggattttttt ctctgtcaat gtatcgactg agctggtagc ttcatctaag atcaacatag
    34201 ggggattact gatcatcgtt cgagcaatcg tcaacagttg gcgttgtcca tctgatatct
    34261 ttaacccttg accgccaatc ttagtatcta atttttccgg caaacgttct acaaattcat
    34321 acatataaga ggcttttagg gcatcattga tttctgcgtc acttgcttcc ggattcccaa
    34381 aacgaagatt ttctcgcaat gtactgtcaa atagccaagt atcttgtaac accattccaa
    34441 aacttttacg taatgtatca cgttggatag agcggatgtc atggtcatcg actgtaattg
    34501 ttccttttgt tgtgtcataa aaacgcatca gcaaattaac aagtgtggat tttccggccc
    34561 ctgtttttcc tactatagca atggtttgac ctggataagc agtaaaattg aaatcttcga
    34621 tcaaacgctg cttggggtca taagaaaaag aaacatgatc aaaagagact tttcctttga
    34681 tatttttggg ttccaaggca taggcatctg gcaaatcagg cttttcttct ggctgatcga
    34741 tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg cagttgtgtc gtgattccag
    34801 aaagttcgat aaatggttta gaaaactgac ttgagtagat cgtaaagctg gaaatcacac
    34861 caacagtgat tgacgaacca ctcttcaatg ccaataggcc gccaactaaa ccaatcgata
    34921 aataagcaag gtgatctaca aaacgagaag acgggtttgt caaggaagaa gaaaattgtg
    34981 cccgttgtcc tttttcatat agatcagcat ttagcagttc aaattgctgt tgactagctt
    35041 cttcttgctg aaaagctttc acgattttct ggttaccgat tcgttcggtc acaaagcccg
    35101 agatatcccc aataatcctt tgctgtctat cgaaatttgt ttgtgaagca cgagcaacga
    35161 tccaactcac aaggaaaata attggtgtag aacaaagaac gaccaatgtc agaagtggac
    35221 ttagacgcat cataaaaata aacgaaagaa tgatgattgc cactccagaa aataattgat
    35281 tgaaggccgc agaaattgca atggaaatat tgtccatatc atttgtaaat cgactgacga
    35341 tatttccgtg ggagttttga tcataataac gaagtggcaa tcgattcaga tgagcaaatg
    35401 catcttttct taattttgca acggataagt aagctaaccg attgcttaac acttggatga
    35461 gccattgact gactacgttg attaaaacga taccaccgaa tagaataagt aaatggaaca
    35521 gtcgattgaa gcggacctgt ccttctccaa tcatttgatc gatactttga ccaatttgta
    35581 acgtcatcac aactgttgca atcccattga tgattcctag tatcacagct agccaaatgt
    35641 cttttgcata ggcagtaagg taaggaatga atcggcggag cgtctgcaag gataattttt
    35701 ttactttcat aactattctt cctcctgtga tgcgacgatt tcctgatatt ctgctgattg
    35761 agcaagtaat tcttcatggg taccctgagc agcaatctta ccttgatcca tcactaagat
    35821 atgatgtgct tcttgaatag agcggacacg ttgggagata ataatcactg tactgccaag
    35881 ataagtctgt agcgccttac gcaaatccaa gtctgtttga taatccaacg cacttaaaga
    35941 gtcatctaaa atcagtaatg ctggcttggc aatcaaagct cttgcaatcg tcagtcgctg
    36001 tttttgtccg ccagagaaat ttttcccatt ttccatgact tttgtatcta aaccatttgg
    36061 aagctgacgg acaaattctt ctgcctgagc gattttcaga gcttcccaac attctttttc
    36121 tgtggcgtct ttcttacccc attgaaggtt ttcacgaata gtcccggtaa atagtacaga
    36181 agtttgggga acaagagcaa tttgctgacg gagatgatcc agctgccaag aacgaacatc
    36241 cattccgtcc attataactg taccggaact gacatcatag aaacgcggaa tcaattgtgt
    36301 caaagtcgtt ttcccgcttc cggttggacc aatgattcca aggactgtat ttcgcttcag
    36361 ctgaaatgta atattttgga gagaaagacc agaatcagga gtataacgga agtcaacatg
    36421 gtcaaaagcg ataattgctt cagaagaaaa atcgggagta gtactgacct cagcttcaag
    36481 aatcgaattt tctgtgtcta gcacctcttt cacccgattt cctgaagctt cggcacgtgt
    36541 gaagatcacg acgaggtttg aaacaacgat cagtgccaaa agcatttgat tcatataatt
    36601 gatcaaagca agaacttgtc cttgttgcaa gctaccaata tttactttga tcccgccaac
    36661 tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt gtagcaggtg ataatagggc
    36721 agagatattg gaaacacgta aataattgtt cgctagatca tttgtgcttt tatcgaactt
    36781 ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca cgaacaccgc taagattctg
    36841 actaacgtgt tcattgagct gatctagttt ttcttgcact ttttggtaga gaggaacggt
    36901 ttttttgatg ataaaaaata aaatgattga gaaaataggc aataatgcta agaaaaacag
    36961 acctacttcc cagtcgatca caaaagccat gaccactgac ccgatactta aaaacggtgc
    37021 tctgatcaat aaacgaatga ccattgctaa agctagctga agctgattga tgtcgtttgt
    37081 cattcgggtg atcaaggtat ctgttccaaa atgattcaac tcgttgtgag agaaagtatt
    37141 gatttttttg attagctgat tacgtaactc tgtaccaaat ccttgtgaag ctactgaagc
    37201 atagtattgg caaatgatag cacaaatcag accaatcaca gacatagcga ccatccataa
    37261 gcccatttca atgatttttc ctcggtcatt catttttagt ccattatcga tcaaggaagc
    37321 catcattaaa ggcaggacaa gttcgaagac agcttcaaga aatttgaaga acgggcccaa
    37381 aatgatttgc ttacgatagt cttttgcata acgtaataac tgaatcatat gattcctcct
    37441 attatttatt tgtccacaat tgaaaaaaca agtactacca cattgtagct taggaaattt
    37501 agaatgaaaa gagaacaagc cttttcataa atgaaagttt tattatgaag aagctttaga
    37561 aattgaatag actatttttt ttaaaaaaaa gaaaatgaga aaacatgcca gtctcttcat
    37621 tcataggaac gtctatttta cttaattttt ctataaatga attctgcata aaaaatatat
    37681 caagtaaatg tttatagatg aaactctatt tacttctttt ttaatttgtt cttgcaatct
    37741 tttttgctac gtaatttttg taaaaaagtg ggttgagaga aatctaattg attttcaaag
    37801 aaggagtatg aaaatgagaa aaatatttat tcgtttattg tgggtactat cttagaggaa
    37861 taaataatag tggagaaaac tatggtttgg cgcagattct atcaggtttg atggaacgaa
    37921 gacaacctcc cgacatcaag tcaaaagcta cttgagattt tgatctgaaa ataaaaaaag
    37981 aaaacaaggt aggaacatca atgtttctac cttgttttcg taatcaatgg gaaaaaacta
    38041 agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca gtacctcttt actctctgaa
    38101 agcatcataa ctcgtttttc ctcattatgc caataaattg ttgttatacc acatataagg
    38161 atttgtaata gctaaaaaca gacaggaaag ttgaaatctg tgaatcttat tttgtatgga
    38221 aggatttata gaacgcaaaa atcaagataa agatgaataa aaatcctgtt tcggctatat
    38281 attgcgtatc tacgccaata aaacgagttt gttctgtcat agccatataa agcaaaggca
    38341 aaaatacaac tacagcgaga taagatccga tgatttctcg aaaagttttc caggaagtat
    38401 gtttcctgtt tttatattta tccgatttca ttgaatagtc atagcctcct tattctttca
    38461 tatgttgaaa ttccagtata aaaactggaa ggaactgtac gttttttaga ataaaaatta
    38521 taagggataa gtaggctgct gtctataata agatatattt ttcaattctt tattaaagat
    38581 tttattgaca aaatgaatcc gttttctatg atggaggagg ggtgaataag ttgttgaaga
    38641 aggaaatttt atgttactta gagaaccaga cagccttttt cgatttagaa catatgaacg
    38701 aggtctttac agcacaaagg ttagctgatc attttggggt aaaaagaaac acagtcagtc
    38761 attatttgaa ccagttaaat gatgagcaac tactagtcaa aatcaattct cgaccagtga
    38821 tttatttcca caaagaagct tttgaaaaac aattttttaa gttacgcacg aatttttatt
    38881 tttctatcga tgatctcaaa gaggaacagc cttttttcgc gcaaccaaaa gacttattct
    38941 cttttatgat cggatacgac gccagtttga aagaaagtat cgaacaaatc aaaacagctc
    39001 tttactatcc agatggcgga ttaccacttt tgattacagg cgaaagtggc acaggtaaaa
    39061 gttatttagt taatttggtc tatcagtatt gtctgcttca tgatctgcta gaagattctg
    39121 caccattcat cacttttaat tgtgcacaat atgcagataa tcctgaactt ttgactagca
    39181 atttgtttgg acatgttaag ggagcgtaca ctggtgcaga agaaaataaa aaaggggctt
    39241 ttgaagcagc agatggtggg atcttgtttt tagacgaggt acatcgctta acaccagaag
    39301 gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat ttatcgtatg ggagaaccta
    39361 atttgtcacg ccggatcaaa acacgtctct tttttgctac gactgaagag attactagtc
    39421 atttcttaac aactttcatt cgaagaatac ctatccgaat cgaactacct gcacttaatc
    39481 agcgtagtcg taatgaacgg ctagaactga tttactcttt ttttctcgaa gaacaaagaa
    39541 aaatggggag ccctttgtca gtcagtggac cagctttgtc acttttaaca agtcagaaat
    39601 tatccggaaa cataggagag ctgaaaaata tcgtcaaagt ttcagcagca aaagcatatg
    39661 ctgaacagcg tgaacagcct gaaatccatg tcacgatcca tcatttgcaa aaagaactac
    39721 ttgcgcagcc tattactaaa aatacagcac agcaaggaat ctatattacg caagaccaga
    39781 ccttagagca attaatggag aagcaacagc ccgaacagca gcgaatcgtt aaaagttttg
    39841 agcaaatcct aattgatttc aagaaaaata actgtctgct atattcttgc gaaggaaaat
    39901 taaagcaaga agtgattcaa ttatttgatt ttttgctgtt tgagaccagt agacaagaaa
    39961 aacacgagct gctggtttat tggacacaat ctatccgaga gacctttcga caaatggaaa
    40021 ctgcctatca aatcaaattc gatggaaaca gtgtctatgc cttgagttac tatctctatc
    40081 agcgaagcac tatcaaatgg ttaccggaag aacatgatat tatccaacta atcaaagaat
    40141 tagaacaaca agtttctcag tcttatccat ctagttatca ctatgtccaa cgaattctgg
    40201 aattaagtaa gatcaaatta gatattgaag acacaagtat ggatcggatt ttactgacga
    40261 tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact gccaaaagcg atgattgctg
    40321 ctcacggtta tgcaactgct agcagtatgg caaatgtagt aaatcgttta ttacaagagg
    40381 atctatttga atcatttgat atgccactag atgtcacacc acagcagatt gcttcagaaa
    40441 tccttgatta ttgcgaaaac agcgatgttt caaatggatt gatcatatta gtagacatgg
    40501 gatctctgaa agaaatccat cagtttttta aaaaacagtt atccgtaccg cttttgattt
    40561 tgaataatgt gacgacacct ttagcaatca cagtagggga atgcctgcaa aaaaatgtat
    40621 cattagaaga gatagcagaa gagactgttc gacagatcca gccagaatgg cgactgctct
    40681 atccagagga aaacaaaccc aaagcactca ttactacttg cttcactgga atcggtacgg
    40741 cggtccactt aagtgaactg ctagaaaaaa gcttacctac ggcttgtcag ttgaaaatca
    40801 tcccatatga atatcagcaa ttaaaagata agaaaaacag tgacccatta ttttccatat
    40861 acgaagtcgt aggaatgatt ggcaccacag atcctgatat caccaatatc gactatcttt
    40921 cattagaaga cctcatctcg ggggaaaaga tgtctgtact agctgaatgg ttggacacta
    40981 ctatgaatgc ttcagagaag gaaatattca atcagcgcat cattcgtaat ttttcattgg
    41041 aaaaagtact ggattccgtg actattctag atacagagaa ggtgatggga gaaatcgatc
    41101 attttatgag agaattagag gtgcagttga atcgaagcat cagcaatccc aaaaaattag
    41161 cgttgtatgt ccatgtgagc tgtctcattg aacgattgat ccgacaaatg ccaatcgaaa
    41221 cctatcaagg attagagaaa tggaagcagt gtcaaaaaga agggttatct gctattaaat
    41281 ctgcgtttag tgtcattgaa gagcattata gtgtcatgat ccccaattct gagattgcct
    41341 atatctatga tattttgagt gaagatacag agtttctctc gatagaagaa gaattttgaa
    41401 cataagcaag ttgttgccac taattttggc atgcaacttg cttttttagt atgtgtaagt
    41461 gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg gaaaatttgc
    41521 ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta ttactgtatt
    41581 cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc tttttatgag
    41641 gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg tcaacaacga
    41701 attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga atttgccact
    41761 attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt tgatccagca
    41821 ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca atcagaaaat
    41881 agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt cgaccatcgg
    41941 ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga ctgtattttg
    42001 attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa attagccaag
    42061 ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc gttgaaaagc
    42121 ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga tgcatataag
    42181 ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa ggcaaaacct
    42241 ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga acgcttgaaa
    42301 cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga taaaaaagtg
    42361 gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag cagtcttatt
    42421 aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt tgctttctcg
    42481 tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa ctgggatcat
    42541 tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg cttcgattcc
    42601 acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag ctggagccgg
    42661 tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga tattgaaaaa
    42721 tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt atgcagaaga
    42781 aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc tttccttgat
    42841 gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg gaaatctatt
    42901 aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt tgattccagc
    42961 tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac catatttttt
    43021 cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag ctatttttgg
    43081 tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac cacttcaaac
    43141 aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc aaaataacga
    43201 aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct tggaattatg
    43261 aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa aagttatatc
    43321 agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt aacacgaccc
    43381 cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa aataaggatg
    43441 atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg ggacctcttg
    43501 cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca ggtgtgggta
    43561 cctctttagc catgcaagga aatattttag gccctatctt gttcattttg attttcaata
    43621 ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta ggaacgggtt
    43681 ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc gcgtctatta
    43741 ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat attcctttaa
    43801 aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat atcgttccaa
    43861 atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa gaagtgaaac
    43921 cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg atagggattt
    43981 tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga agaaaaggaa
    44041 gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat gaaaaaaaca
    44101 gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc agcgaagcca
    44161 gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta ttttttcaat
    44221 tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag cgggaaaagt
    44281 gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt tgttgctatc
    44341 acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga tttaggtgcc
    44401 ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt acagttacta
    44461 ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat agaacaagac
    44521 tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca aaaaacggaa
    44581 gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt caccattgga
    44641 tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga aacaatccgc
    44701 gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct ggaggcagat
    44761 gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg ttcgcaaaag
    44821 ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac aaaaaaagca
    44881 agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc tgtattagcg
    44941 cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg tattgacttg
    45001 aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata agaggtggaa
    45061 atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc actagcttta
    45121 cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga cgctgtctat
    45181 tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta tatgaatggg
    45241 aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac tggaaacaaa
    45301 agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac tcaagaagta
    45361 gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt tatcgataaa
    45421 gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc tcctggaaca
    45481 gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg agaattcgaa
    45541 gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt agtggaagca
    45601 gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg tgatagtatg
    45661 cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc catgtgttac
    45721 tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt tcttcacggg
    45781 acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga agatgaacag
    45841 agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa ttatacattg
    45901 atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg acgtgttttg
    45961 tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga gcagttgaac
    46021 tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga tagactggat
    46081 aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt ttttaaaata
    46141 ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca acttataatc
    46201 tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac tatttagttg
    46261 ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta catagcgttt
    46321 tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca gagacaatct
    46381 gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg tattttaaat
    46441 caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag taacattgtc
    46501 aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa aatgtagact
    46561 attgtaacat atcattgatt atttcaagta aaaacgataa aagaagtaaa caaacactta
    46621 aaaacaatgg aaattagtcc caactttaag aaatgtatac tataatggaa attatcaatt
    46681 atttatttgg aggaatatcc atgaaacaaa aaatcgcagt tcttggtcct ggatcttggg
    46741 gcactgcttt agcacaaaca ttggcagaaa acgggcatga tgtccgtatt tggggaaatg
    46801 tacctgaaca gattgatgaa atcaataagt atcatacaaa tcagcatttt ttacctgatt
    46861 tgacgatccc agaatcgatt atcggatata aagaattggc agaagcagtg gataacgcag
    46921 atgctatttt atttgttgtt ccaacaaaag cgattcgttc agtcgcacag gaacttattg
    46981 gtaaaatgaa tactaagcca atcattatac atgcaagtaa aggactagaa caagatacgc
    47041 ataaacgaat ctcagaagtt ttggctgaag aaattccaga agaaaaaaga caggcaattg
    47101 ttgtattatc tggtcctagc catgctgagg aagtggcagt acatgatatc acaacaatta
    47161 cagctgcaag tattgatcaa aaagcagcag cctatgtaca aaaactgttc atgaatgatt
    47221 atttcaggat ttatacaaat aatgatgtga ttggcgtaga aactggtgca gcactgaaaa
    47281 atatcattgc aattggtgcc ggcgctatcc atggattagg attcggagat gatgcaaaag
    47341 cagcaatcat gactcgagga ttggcagaaa tcagccgtct tggtgtcgcg atgggtgcca
    47401 atcctctgac atttatcggt ttgagtggtg tgggggactt gatcgttact tgtacaagtg
    47461 ttcattctcg caattggcgt gccggtaact tgttaggaca gggacataag ctggaagaag
    47521 ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac gacaaaagca gcagtagagc
    47581 tagctcaaca gctaggtgtt gagatgccaa ttactcaaac aatctataat gttttgtata
    47641 atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt acgtgacgga aaaatggaga
    47701 atgaattttc tcttcgttaa atagatgatc aatagttaat cgt
//
LOCUS       DACDBG010000016        47283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDBG010000016 DACDBG010000000
VERSION     DACDBG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.016"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(30..881)
                     /locus_tag="IVZ90_000846"
     CDS             complement(30..881)
                     /locus_tag="IVZ90_000846"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8261914.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1035..3608)
                     /gene="mprF"
                     /locus_tag="IVZ90_000847"
     CDS             complement(1035..3608)
                     /gene="mprF"
                     /locus_tag="IVZ90_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8261915.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3749..4555
                     /gene="proC"
                     /locus_tag="IVZ90_000848"
     CDS             3749..4555
                     /gene="proC"
                     /locus_tag="IVZ90_000848"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8261916.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4634..6190)
                     /gene="rny"
                     /locus_tag="IVZ90_000849"
     CDS             complement(4634..6190)
                     /gene="rny"
                     /locus_tag="IVZ90_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8261917.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6601..7650)
                     /gene="recA"
                     /locus_tag="IVZ90_000850"
     CDS             complement(6601..7650)
                     /gene="recA"
                     /locus_tag="IVZ90_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8261918.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(7759..9000)
                     /locus_tag="IVZ90_000851"
     CDS             complement(7759..9000)
                     /locus_tag="IVZ90_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8261919.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(9240..9818)
                     /gene="pgsA"
                     /locus_tag="IVZ90_000852"
     CDS             complement(9240..9818)
                     /gene="pgsA"
                     /locus_tag="IVZ90_000852"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8261920.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(10027..10911)
                     /locus_tag="IVZ90_000853"
     CDS             complement(10027..10911)
                     /locus_tag="IVZ90_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8261921.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(11689..12975)
                     /locus_tag="IVZ90_000854"
     CDS             complement(11689..12975)
                     /locus_tag="IVZ90_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8261922.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(12968..14236)
                     /locus_tag="IVZ90_000855"
     CDS             complement(12968..14236)
                     /locus_tag="IVZ90_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8261923.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            14488..14949
                     /gene="mscL"
                     /locus_tag="IVZ90_000856"
     CDS             14488..14949
                     /gene="mscL"
                     /locus_tag="IVZ90_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8261924.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(15009..15455)
                     /locus_tag="IVZ90_000857"
     CDS             complement(15009..15455)
                     /locus_tag="IVZ90_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8261925.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(15548..15793)
                     /locus_tag="IVZ90_000858"
     CDS             complement(15548..15793)
                     /locus_tag="IVZ90_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261926.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(15909..16487)
                     /locus_tag="IVZ90_000859"
     CDS             complement(15909..16487)
                     /locus_tag="IVZ90_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8261927.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(16564..17094)
                     /locus_tag="IVZ90_000860"
     CDS             complement(16564..17094)
                     /locus_tag="IVZ90_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8261928.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(17098..17958)
                     /gene="rlmB"
                     /locus_tag="IVZ90_000861"
     CDS             complement(17098..17958)
                     /gene="rlmB"
                     /locus_tag="IVZ90_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8261929.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(17951..18346)
                     /locus_tag="IVZ90_000862"
     CDS             complement(17951..18346)
                     /locus_tag="IVZ90_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8261930.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(18343..19755)
                     /locus_tag="IVZ90_000863"
     CDS             complement(18343..19755)
                     /locus_tag="IVZ90_000863"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8261931.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(19752..20288)
                     /gene="cysE"
                     /locus_tag="IVZ90_000864"
     CDS             complement(19752..20288)
                     /gene="cysE"
                     /locus_tag="IVZ90_000864"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8261932.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(20610..22070)
                     /locus_tag="IVZ90_000865"
     CDS             complement(20610..22070)
                     /locus_tag="IVZ90_000865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8261933.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(22240..23397)
                     /locus_tag="IVZ90_000866"
     CDS             complement(22240..23397)
                     /locus_tag="IVZ90_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8261934.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(23431..24801)
                     /gene="radA"
                     /locus_tag="IVZ90_000867"
     CDS             complement(23431..24801)
                     /gene="radA"
                     /locus_tag="IVZ90_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8261935.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(24864..25391)
                     /locus_tag="IVZ90_000868"
     CDS             complement(24864..25391)
                     /locus_tag="IVZ90_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8261936.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            25621..26427
                     /gene="proB"
                     /locus_tag="IVZ90_000869"
     CDS             25621..26427
                     /gene="proB"
                     /locus_tag="IVZ90_000869"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8261937.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            26424..27671
                     /locus_tag="IVZ90_000870"
     CDS             26424..27671
                     /locus_tag="IVZ90_000870"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8261938.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            27747..28091
                     /locus_tag="IVZ90_000871"
     CDS             27747..28091
                     /locus_tag="IVZ90_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261939.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(28208..28534)
                     /locus_tag="IVZ90_000872"
     CDS             complement(28208..28534)
                     /locus_tag="IVZ90_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8261940.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(29149..30351)
                     /locus_tag="IVZ90_000873"
     CDS             complement(29149..30351)
                     /locus_tag="IVZ90_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8261941.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            30468..30983
                     /locus_tag="IVZ90_000874"
     CDS             30468..30983
                     /locus_tag="IVZ90_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8261942.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(31182..31628)
                     /locus_tag="IVZ90_000875"
     CDS             complement(31182..31628)
                     /locus_tag="IVZ90_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8261943.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(31675..33153)
                     /gene="msr"
                     /locus_tag="IVZ90_000876"
     CDS             complement(31675..33153)
                     /gene="msr"
                     /locus_tag="IVZ90_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8261944.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(33507..34136)
                     /locus_tag="IVZ90_000877"
     CDS             complement(33507..34136)
                     /locus_tag="IVZ90_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8261945.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(34120..34584)
                     /locus_tag="IVZ90_000878"
     CDS             complement(34120..34584)
                     /locus_tag="IVZ90_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8261946.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(35071..36339)
                     /locus_tag="IVZ90_000879"
     CDS             complement(35071..36339)
                     /locus_tag="IVZ90_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8261947.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(36570..37307)
                     /locus_tag="IVZ90_000880"
     CDS             complement(36570..37307)
                     /locus_tag="IVZ90_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8261948.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(37374..37943)
                     /locus_tag="IVZ90_000881"
     CDS             complement(37374..37943)
                     /locus_tag="IVZ90_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8261949.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            38071..38943
                     /locus_tag="IVZ90_000882"
     CDS             38071..38943
                     /locus_tag="IVZ90_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8261950.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            39285..40157
                     /locus_tag="IVZ90_000883"
     CDS             39285..40157
                     /locus_tag="IVZ90_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8261951.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            40154..42112
                     /locus_tag="IVZ90_000884"
     CDS             40154..42112
                     /locus_tag="IVZ90_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8261952.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(42444..43295)
                     /locus_tag="IVZ90_000885"
     CDS             complement(42444..43295)
                     /locus_tag="IVZ90_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8261953.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            43418..43885
                     /locus_tag="IVZ90_000886"
     CDS             43418..43885
                     /locus_tag="IVZ90_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8261954.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(44148..44630)
                     /locus_tag="IVZ90_000887"
     CDS             complement(44148..44630)
                     /locus_tag="IVZ90_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8261955.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(44730..45593)
                     /locus_tag="IVZ90_000888"
     CDS             complement(44730..45593)
                     /locus_tag="IVZ90_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8261956.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            complement(46195..47274)
                     /locus_tag="IVZ90_000889"
     CDS             complement(46195..47274)
                     /locus_tag="IVZ90_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8261957.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT        13404 a        10146 c         8538 g        15195 t
ORIGIN      
        1 ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa ataaacttcc
       61 tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg ttgacacaag
      121 gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta cttgatcata
      181 cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc taaactgttt
      241 cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc aagtccgtgg
      301 atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg tgtagagaca
      361 ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa tacttggatt
      421 tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg aacatctgta
      481 ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt ccataattgg
      541 ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc agctgctact
      601 ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt attttttagc
      661 aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc tttatttgtt
      721 tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc caagtctacg
      781 ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa tacatccagt
      841 cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc cattcgaaaa
      901 gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat gtctgcctag
      961 tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat aatccaaaca
     1021 agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg aacatttcct
     1081 ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt acttcctttc
     1141 ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt ttcataagca
     1201 gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac tttttcttga
     1261 aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag attggcttcc
     1321 gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc agctaattcc
     1381 tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc
     1441 tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac accgctagat
     1501 aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc accttgaagc
     1561 catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg atcgtaccaa
     1621 gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact cagccgagac
     1681 aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc
     1741 aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag ctgatagcct
     1801 aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt aggattgcca
     1861 actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa aacgacttga
     1921 tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag ctgatggctt
     1981 gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc gtactcactg
     2041 ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc tagcaatgag
     2101 atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa aataaaatga
     2161 tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg
     2221 ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga ataaacgaat
     2281 tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa gacatatacc
     2341 aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa agatagtaaa
     2401 gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc aaggccgagt
     2461 aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga gaacggcaat
     2521 aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag aagtaccatc
     2581 atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt ttggaaaaac
     2641 agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt gatgaacaat
     2701 agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa tagccaaact
     2761 actgctacat cttgccgaac agacaattga ctcattccta agatcatcaa aacgtcaaaa
     2821 gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat catgaacata
     2881 ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat caaaaatacc
     2941 gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa gccttttgga
     3001 taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat gactggtgct
     3061 aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc tgactgaata
     3121 aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag cataaatagt
     3181 gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc ataaaaacta
     3241 gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat cgtgtttgtt
     3301 acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg tttttctaag
     3361 actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag tccagccaga
     3421 atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat atcttgccaa
     3481 ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac ggagccgaaa
     3541 aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa gtatcgttta
     3601 tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca atgttatctt
     3661 atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag aaaaagaaag
     3721 atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca cagggaatat
     3781 ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt
     3841 cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc
     3901 atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag ccgtgaaacc
     3961 aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag tcttattcat
     4021 ttctattgct tcaggaaaat cattaataga tttagaaaca gcgcttgaag atcctcaggc
     4081 ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat
     4141 ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga tttttgaagc
     4201 aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg gccttgctgg
     4261 cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg gtgtactgaa
     4321 tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg gttcttgtca
     4381 aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt cttctcctgg
     4441 cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc cagcaatcat
     4501 caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa aataatgata
     4561 gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg cggctcttct
     4621 tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata acgattactt
     4681 tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca cgtactaaac
     4741 gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact tcgcgtccag
     4801 cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt tccagacttc
     4861 ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt gctgctgaga
     4921 gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca tctccatgat
     4981 gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct aattccgcac
     5041 cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata tcatggagca
     5101 acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag ataccagcta
     5161 attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca
     5221 gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact tcaaacgctg
     5281 cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc tcgaccattt
     5341 cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag gtcatccgtg
     5401 cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt gtatcatcga
     5461 tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct tctcgtccaa
     5521 tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa
     5581 cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct ttgcgatctg
     5641 cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca tgattcagct
     5701 cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa ccagcaattt
     5761 tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct cgctcttcaa
     5821 ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc tcgcgttttt
     5881 ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag
     5941 attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg tatttctgat
     6001 tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct ttttttgcac
     6061 tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg tgacgtgact
     6121 ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg aggagaatgt
     6181 tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct atacagcttc
     6241 tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca
     6301 tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac
     6361 ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag tttcatatag
     6421 gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg aacagatatt
     6481 tttattttat gtacccactc gtataattaa ataactcacg atttctttat tcaataaaaa
     6541 aacggcaggt caaggagagt acctactctt cagaccagcc gtttttgttt atcataattt
     6601 ttattcttct actaaaggca attcttcttg tcctttttca tcttctggga cagcgacttc
     6661 ttcaccaata ccataagctg ctctaactag agcagaaact tctgccatca tttcaggatg
     6721 attagccata tagattttag cgttttcccg tccttgacca atccgatctt ctttataaga
     6781 ataccaagca ccgcttttat caacaatgtc tttttctact gccatgtcta gaagttctcc
     6841 ttcttgtgag atccctaaac catacatgac atctacttca gcgattttga aaggaggtgc
     6901 tactttgttt tttacgactt tgatctttgt acggttacca acaatatctg taccttgttt
     6961 caattgttct gcacgacgta cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc
     7021 tccaggagtg atttctggat tcccaaacat cacgcctact ttttcacgga tttggttgat
     7081 gaaaatagcg attgtttttg ttttgttgat cgaaccagag agtttacgca atgcttgaga
     7141 catcaaacgt gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga tttctgctcg
     7201 tggaactaaa gcagcaactg aatcgactac tacgatatct acagccccac ttgatactaa
     7261 agcatcagcg atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc
     7321 gatattcaca cctaattttt gtgcatattg cggatctaac gcatgctcag catcaatgaa
     7381 agcggccgtt ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt
     7441 acctgaactt tcaggtccat atacttcgat gatacgtcca cgggggtaac cgccgacacc
     7501 taaagcgaca tctaacgcaa gagagccact tggaattgta gagatttgtt ggtcgatttt
     7561 ttcgcccaat ttcataatag agcctttacc atagctcttt tcaatttttt tcagtgcagc
     7621 atctaaggct gctttacgat catctgccaa tacgataatc ctccttatat ttacaaatca
     7681 gagcaacatt tcattatcct tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg
     7741 aacaaatatt cgcatttatc attttcttaa aagctgtttt ctcaaaagat caaatccttg
     7801 cataaccgca ttatatctga tagcagcacg atctctggca aagtgatatt tttccgaaac
     7861 cacgccgtct ttagatgaaa tagcgatcca tactgttcct gcagattgac cttctaacgt
     7921 gtccggtcca gccactccgg tgaaagctac actgtaatct gttcttgcta tctcacgtgc
     7981 ttgaatggcc atctgctcca cacattcttt gctgactgtt ccatattctg ctaatagctc
     8041 cggatcaatc tccagaaaac cagcttttgt ttggagcgag taggtaacga atccgcctgg
     8101 aaatacttca gatacaccag caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa
     8161 actttccgct gctgttacag tctgcttgtt ttctttcagt agttccacta cgacttttgc
     8221 tagcgaatta tcatcgccgt atccataaaa atattcccct actcgttctt ggattttttc
     8281 ttctaatgct aaaagagctt gattccctgc ctgaacatca tttgttttca ctgtcagtcg
     8341 taatgtcact tcatttggtt tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat
     8401 cagatctttt agctcagtga ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt
     8461 agaaattagt ttttcttcag aaggaaaacg ttcctctagt aaaggacgta cttgttccac
     8521 aaacattggt ttcaactcat taggcggacc gggaagaagg atatacgcat gtgtatctgt
     8581 ttgataaaaa gtccctaaag caagccctgt acgatttggt agaggaaccc cgccttcgat
     8641 aatctgggac tgttgcagat tgtttttggt catttttcga tgggttgttt caaaatatgc
     8701 caataatttt ttgtatcctt ccgtattttg tatgagagac ttaccgaggt gagccgcagt
     8761 tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg ccacataaga gtatgagttc
     8821 acttcttgtt tcagctagag caatcaactc ttctaaacgc tgctgattat ctcctacaac
     8881 tgtttgataa taaacttcga tacctagatc agctagctgt tctgacaaga aagtggcatt
     8941 cgtattcacg acttgtccta gtaaaagttc tgttccgaca gctattattt ctgctttcat
     9001 tacttcccgc tcccttatat agtatacgct gtatttttat atttttttat ttaaaaagag
     9061 ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt tccaaataaa cagtgttctc
     9121 ctgccaaaac ctcggtcata agtcacaata tacataaaac atgaaaagct gttttttgaa
     9181 attatccctt gtgactcaga gttagacggc aggctcacaa cctctccact aaacgatact
     9241 tacattgatc ctttgaacac accgctgttt ttgatgaagt aatccacacc tgaatagata
     9301 gtaaagatca gacaagcata aagcatgatt tgatctactg gaataccgat caggctgaat
     9361 gggatattat tgataaacag caagataata gcaaccattt gtgtagccgt cttgactttt
     9421 cccggccaag ctgcagccat gacttctcca tgttcgacaa gtagtaaacg caagcctgtg
     9481 acggctaatt cacgacagac aatgatcgct actacccaac caggggcctt tccttggtca
     9541 accaacatga taaaagcagt cataactagc attttatctg ccaacgggtc cgcaaacttg
     9601 ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc catctaacca gtctgtgata
     9661 ctagctaaag caaaaataac tgcacctact agctgtgtga cagccagtgt ggtgtctcct
     9721 acgttaaatt gtccccaatc taaaggtaaa cctacaacca aaatgaaaat cgggatcata
     9781 caaattctaa gtacagttaa tttatttggt aaattcacac tatcgactcc tttcttccct
     9841 attctacgtg actatcagcg aagtttccac cgatattatt tacttttcca aatgaattta
     9901 ttcaaaggag tatttttatt gaaattgatc tttttgaatt aacgaagaag gaccgcgaca
     9961 cagatttgtc acgatccttc ttttttatac ctacaagtaa acagatgcgt aaactaattt
    10021 atacgatcat tctgcgtatt gaagagtaag gttcacattt tttggactct gattgttttc
    10081 ccctggatta actggtacgg attctccatt cgctttgatc gttgcattgc ttgctgcacc
    10141 aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg tttcatttgc
    10201 tgccagtgtc ccttgataaa cataggcatt gtctactagc acacctaacc atacacgatt
    10261 gacagcagta aattcaaggg ttacaggctt ggtagcgtct gtgatattta ctgcgatggc
    10321 actgccagaa tcttctcctg tagctattgc cattttcttt tcttcacttg attttgttgt
    10381 tgattctact gtagaagact ctttggtctg cgtgctttgc tctgaggaag aggactcctc
    10441 gactgatcct tctactttaa gcgaagaagc tgtgtctccg attattggat cagcttgacg
    10501 atcttgccaa gtcatatagc ccacgacaac gacaatcgcc aaagcaacca atcctaataa
    10561 aatgacagga agacttgtcc aaaagcgact tggatgcgat tcttcctgat gcattgcttt
    10621 tctagaagta cttactgttt caggctgcgg acgttcaggg accgatcctg tcaatttgct
    10681 tttcccatca aaaacatcga ctaaatggtc tccatcttct cctacagctt cagcgtattg
    10741 ccgaataaat gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat cattgtcgat
    10801 tgcttccaaa taacgttttt gaatttttgt aatctgctgt aattcatcta aagatatttt
    10861 tttattcagg cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc
    10921 ttctccagtc tgtttgattc tccgaagcat ctgctgttgc aacgaaagac gttatgtttg
    10981 ggtgctgtga caaaagtcat gtcacagctc cttttttccg aataaacatt gttctcctgc
    11041 caactcctcg gtcacaagtc acaatatcca ataaatataa gaagctgttt tttgaacttg
    11101 ttccttgtga ctcggagtta aacggcaggc tcacgacctc tttcttaaac ggtgttcaat
    11161 cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat
    11221 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa acggtgttca
    11281 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acattaagaa gctgttttta
    11341 cttggctgtt cttattactt gatgcagggc agctttttca caacctcttg gtacacggtg
    11401 ttccagaagt agcttcttcg gcaataagcc gaaatatcac aaaaatttga gaagcaattt
    11461 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc tttgattact
    11521 tcgtttgatt ttattttaat ataagttgat taaaagagag catgatttgt tttattttct
    11581 cactaatttt tccaacacaa ctaagaatac cataaaagct gattttggag gaggttccta
    11641 ctacaagtct aaaacgaaat ttaagacttt tcctcataaa atactggttt attttggtct
    11701 catgtaaaac tcgctgaagg cctcttcact tataaatgct tccccagctg ccagtacgtc
    11761 tttcatttgg atcgaatcga tgatttcagg aagatcaaat aaagttcgat cgccaaataa
    11821 actttgcgta aattgattgg caatatattc gatcgaattt aatgattgga aatattgtcc
    11881 aagcattttc ttcttcagca aatcgagatt tgtttctgac acttccggat catcagcgaa
    11941 tccaagaatg atttctttga ctttttctgc tgctttctct ggttcgtccg tatctccgct
    12001 gaagtccgca aagtgaaatt cacgatccaa gctgaattca aaaccaaagc tgtcatcgat
    12061 aattccttga ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa
    12121 caacagattg attgctgttt tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc
    12181 taatccttta ataccaagta caaatttatc tcgtgtaatg gctgcttcca atgcactttg
    12241 tttgatgatt tcttttgtgt tttcagggaa ataacgtaca atttcttgct ttggagggaa
    12301 attttttgct tcttggtttt cccgaataag tttcatcaat ttctcaggct ccatcttacc
    12361 aactacaaat aaaaccatat tgcttggctg ataaaacgtc cgatagcacg tatacaagtc
    12421 ttgagctgtg attttgtcga tactttcaac cgtgcccgca atatcgatat gcaatgggtg
    12481 tgtaggatac aagttattta gtatgccaaa aaacatccgc cagttagggt cgtcttcata
    12541 catttggatt tcctgaccga tgattccttt ttccttgttt acagtttctt ctgtgaagta
    12601 aggtgcttgt acaaaatcga tcagagtcgt cagattcttt tctacctgat ccgtcgtaga
    12661 aaacagatag ctggttttcg taaaacttgt gaaagcattg gcggaagctc cttgtttccc
    12721 aaataattga aagacatcac catcttcttt ttcaaacaac ttatgttcta aaaaatgggc
    12781 aattccatct ggaactttca ccttttcttt ttcaccatag ggaataaatt cgttatcgat
    12841 cgaaccataa ttcgtactga acaaaccata tgtcttgtga tagtcatttt taggcaacaa
    12901 atacacggtc aaaccgtttg gcagtacttc atgataaagt gtttcattga tttgctcata
    12961 ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc aattcgattt
    13021 gctcagctac ttgttgtatc tctttgatcg ttacgctctc catacgtttc aaccattctt
    13081 catcagataa ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg attgccgctt
    13141 gcggagagtc tagagaaagc agatattggt ttctcagcat tgcttttgtc tgagacagtt
    13201 ccaactctgt tatttcacca tttcgcaaac tttctaattg ctcgtgaatc agatgaagta
    13261 cttgatttcg gttcttttca tcgataccgg tttggacact catgaatcct cggaacgtat
    13321 ccacgctact agaggcataa taagctagac tttctttctc tctgacattc atgaaaagtt
    13381 tagagtgagg aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa cgttctggtt
    13441 catcgtaata cacatttgtc tgataagcta ggttcagctt tgcttgaacg attggctcac
    13501 gtacttgtcg ttccttcacg atattcactt gaggttgggt ataaaacaga tctggataaa
    13561 ctgtttcccg cgactcaaat ggtaattgcc caactgcttc agctgctttg tccggatcaa
    13621 catcgccgac aacaaaaatg tcgatttgat catttgccat catttcttga tagtaggacg
    13681 ctagcgaact agcagtgatc ttgtccaaat cttcgactac gcccaaccct gggatctttt
    13741 gttccgggct gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa gtttgtttat
    13801 cttccttcaa actttccaaa tatagacgca gattgttctt ttccaagtca aacgtcaact
    13861 gatcaaactg atgattttta atatttggat aaaataaaac ctcttttaaa aaatctaccg
    13921 cttgagaaaa aaggtttgga tcattgacat attcaccatt cacaaaactg atccctgcgt
    13981 ttagccaatg taaatttcct ttttttctaa ctgataatcc aaagcttgcg ccataaagct
    14041 ctgcaagttg cgcacttaac tttgtctgat ccgaataatt caaactattt gtttccaaca
    14101 tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga
    14161 taaaaatccg gatcgttttg tattgttttg ttggtaagac atgaagattc acgccttctg
    14221 ccaattgata caccatattt atatgctcct tttcacagtt gattgcccgc ttactctttt
    14281 aatgggtcaa cctgcctttt ttacttattc ctctgcttga aatttataag ttcaggcaat
    14341 tcgccgtttt ttctaaatga tgttacactt tatagtatag catagatgtt ttttatcaaa
    14401 agaaacagac ggctaaaagc gtatattgtt tttttatttt ttatttttcg atatactaaa
    14461 gacaaataaa agaagggagg gtcattcatg atcaaagaat tcaaagagtt catcatgcgt
    14521 ggagatgtct tggatcttgc tgtcggcgtt gttattggta gtgcgtttac aagcatcgta
    14581 aaccaagtgg tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc aatgatcaca
    14641 ggtacttctg atttagaagg agcactttca attcttgact ggtcgccgac aaaaggcgtc
    14701 acatttgcgt ttggtaacgt tattagtgct atcattacat ttctgatcac tggttttgtc
    14761 ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa
    14821 gtggaagtgg aagaaactcc tactgcagaa gattatctta gtgacatacg cgatcttctt
    14881 gccaaacaag tggcagaaga caatacacat ccagtagagc aaacaaatct ttccgatact
    14941 tctaaataat aaagaatcta aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt
    15001 ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat atcatcttcc
    15061 aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg taggtattct
    15121 tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt aatcgtttct
    15181 tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag gacacgtcca
    15241 aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt cccatcagta
    15301 tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg ataaggacac
    15361 tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt
    15421 gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc tgctttttta
    15481 ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt
    15541 ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg
    15601 aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata
    15661 ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct
    15721 gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta
    15781 aagttgttgg catacagttc acctctacta tcaactatac cacatatttt taaaattttc
    15841 aagtttacca taaaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt
    15901 tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc
    15961 ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa
    16021 tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg
    16081 caattttcga ttgcttcttt taataaaaga tgctgaattg ctaaaggttc ttgcaatgtg
    16141 atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct
    16201 ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt
    16261 ataacgcttt tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat
    16321 gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa
    16381 taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat
    16441 gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc
    16501 cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat
    16561 atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc
    16621 ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc
    16681 tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc
    16741 tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt
    16801 caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc
    16861 tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc ccggaactag
    16921 ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg
    16981 atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc
    17041 cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta
    17101 ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt
    17161 taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa
    17221 tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt
    17281 ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt
    17341 gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc
    17401 cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat
    17461 cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc
    17521 ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat
    17581 cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac
    17641 gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg
    17701 ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa
    17761 gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa
    17821 attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt
    17881 tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt
    17941 tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt
    18001 ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg
    18061 cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc
    18121 ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa
    18181 tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg
    18241 gttttgtttc tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc
    18301 caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca
    18361 tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat
    18421 ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg
    18481 ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca
    18541 ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag
    18601 ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa
    18661 atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc
    18721 cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt
    18781 attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat
    18841 cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt
    18901 gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc
    18961 gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc
    19021 ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg
    19081 gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc
    19141 gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca
    19201 taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc
    19261 tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg
    19321 ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg
    19381 aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggttc
    19441 gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg
    19501 tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt
    19561 cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc
    19621 aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata
    19681 cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt
    19741 gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct
    19801 ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg
    19861 attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg
    19921 gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt
    19981 acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc
    20041 attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca
    20101 gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat
    20161 aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc
    20221 tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac
    20281 caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa
    20341 agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca
    20401 cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta
    20461 ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga
    20521 ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc
    20581 agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat
    20641 gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct
    20701 gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga
    20761 acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt
    20821 gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct
    20881 tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca
    20941 tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca
    21001 tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat
    21061 gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg
    21121 aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt
    21181 tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt
    21241 gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca
    21301 cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt
    21361 ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg
    21421 tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa
    21481 ttataagttg gcataccatc gcgtttttgg atgacaaagt cgccaccgat attatcggat
    21541 tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca
    21601 cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc
    21661 aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt
    21721 tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc
    21781 tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct
    21841 ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg
    21901 cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag
    21961 tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata
    22021 tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc
    22081 ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata
    22141 tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca
    22201 actggttgaa tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt
    22261 tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat
    22321 catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat
    22381 gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg
    22441 ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac
    22501 tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga
    22561 gactttattc aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt
    22621 gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga
    22681 tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg
    22741 tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat
    22801 catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat
    22861 cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag
    22921 ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac
    22981 tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat
    23041 catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt
    23101 cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata
    23161 agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt
    23221 ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt
    23281 attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga
    23341 aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt
    23401 ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc
    23461 caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt
    23521 tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat
    23581 atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg
    23641 ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc
    23701 tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag
    23761 caacccagct cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc
    23821 tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac
    23881 caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag
    23941 gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat
    24001 ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg
    24061 aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat
    24121 tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc
    24181 atttgtttta gcggttttga gtagttctgc agtggtttct cttacttggc tgacacttcc
    24241 tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc
    24301 aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata
    24361 aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc
    24421 ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa
    24481 cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac
    24541 cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt
    24601 tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg
    24661 agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca
    24721 atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg
    24781 tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat
    24841 caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga
    24901 gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc
    24961 tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg
    25021 ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac
    25081 actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct
    25141 cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct
    25201 gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc
    25261 cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt
    25321 gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct
    25381 ctttttctca ccggttttcc tccctaaata aactactcct attctaacat atccgaggct
    25441 gtaaaagact ttctttacaa attcttcaag aaattctgct atcttaaagt aaaccattaa
    25501 ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta catgacttta
    25561 ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg atatttgatt gaggtgtcac
    25621 atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac aagtacattg
    25681 atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt tgtcctttct
    25741 gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt aggcgttggt
    25801 atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca agctgcggcg
    25861 gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt atataatcaa
    25921 cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag tcgtaaaaat
    25981 gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt gaacgaaaac
    26041 gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa tgatcagctg
    26101 tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc tgatatcgac
    26161 gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg ctatatcgat
    26221 aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt tggaacaggc
    26281 ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag cgcaatgatt
    26341 ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa agaagtaggt
    26401 actttattta aggaggactt gaaatgactg acttgatgca attaggacaa caggcaaaag
    26461 atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta cttgttcaaa
    26521 tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat aaagacttag
    26581 aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg
    26641 agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac gatcctattg
    26701 gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa aagcgcgtgc
    26761 cactaggcgt gatcggcatc atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta
    26821 gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa gcattcttct
    26881 cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc gaatctcctt
    26941 atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg atgcgattga
    27001 cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa cgagtcaaag
    27061 aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta tatatcgata
    27121 aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat
    27181 ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct tttttcttgc
    27241 cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt
    27301 tagaatatct agagacggct gctttagcag aagacacaga ttgggatact gaatatctag
    27361 attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca catatcaatc
    27421 ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct agtcaacgtt
    27481 tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt tttacagacg
    27541 gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg catgcccgtg
    27601 gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc gatggacaaa
    27661 tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg
    27721 ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt tttatttacc
    27781 ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt ttctttcaat
    27841 tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg
    27901 ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta tgcttattag
    27961 cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt acgctactga
    28021 ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac ttgaatcgga
    28081 ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg caaaaaactg
    28141 ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc atataaacgg
    28201 cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt
    28261 caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc atcgtttttt
    28321 ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga tacataccag
    28381 ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt ggcaattctt
    28441 tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg
    28501 tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt ctttttctta
    28561 aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag aaaaaaaggg
    28621 aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc ttctgccaac
    28681 tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg aaattgttcc
    28741 ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt tcaatcagct
    28801 gaccctcggt attagctcaa aaaacggtaa aatatgagaa gctatttttc cgttttgttt
    28861 ctctaatact taggataaaa cgctgatttt acaacctctg ttaaacggtg ttcaatcagc
    28921 tgtccttcgg tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt
    28981 ctctaatact caggacaaaa cgctgatttt acaacctcct gataaacggt gttcaaaagc
    29041 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    29101 tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc
    29161 atttgtagca aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc
    29221 acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa
    29281 tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg
    29341 ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa
    29401 aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa
    29461 taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa
    29521 cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc
    29581 aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc
    29641 aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt
    29701 atgattgctt aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt
    29761 ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa
    29821 aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat
    29881 tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat
    29941 actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc
    30001 acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac
    30061 gatgataaac aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc
    30121 tgtaaatagc atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc
    30181 agctggcgta gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac
    30241 tcccataaaa gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt
    30301 ttcttcattt gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc
    30361 tctattagca tcatacatga tttattttac atgtcaatat gcacatgtaa aacgtggaaa
    30421 ctttactgtg atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag
    30481 cctatatatt attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc
    30541 tgatcacaat tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag
    30601 accagcaggg ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc
    30661 acttgactga tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc
    30721 ctacgcaaaa agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa
    30781 cagctactgg actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc
    30841 aaaaaaaatt gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga
    30901 tccagcgcag aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac
    30961 aaatgcagca gttttttgag taagggataa acagaacctt tctagaaata tctattgaag
    31021 agcaaattat cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac
    31081 aggccaaaac ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac
    31141 aaaaagcatg ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt
    31201 gcttttagca cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca
    31261 atcacattca ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt
    31321 tccatgatca caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa
    31381 atagttaaga tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt
    31441 ttcgccagca caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact
    31501 agaccaatca acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc
    31561 atccccaaga ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt
    31621 gtaggcaatc tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa
    31681 atttctcgta agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt
    31741 acgctcgaca aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat
    31801 tagcttctct aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag
    31861 caacacatta cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc
    31921 acttaatgta cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa
    31981 actacggaca agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc
    32041 ttcagatgtc atttgataac ccttttgctg atatacttga aagcttactt tcggagagag
    32101 aaggattcct tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt
    32161 ttcaccgaca atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa
    32221 tgttctccca cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag
    32281 ttcaagagat tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc
    32341 tcctattttt tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac
    32401 actgtctttt tgtttagagg aagacaaccg atccagtctg atttgttgct ttttcttttt
    32461 actggacact ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa
    32521 tcgttttttc tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc
    32581 cttttgagtg agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac
    32641 ttcccagatt ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag
    32701 cgtaccataa taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg
    32761 ggttgttggc tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc
    32821 gatctttcgt tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg
    32881 gaaatgactg gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg
    32941 caggtagttg aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc
    33001 tattagtttg agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc
    33061 ttgctgatag acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg
    33121 tagatttgtt atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa
    33181 acaacaatta tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac
    33241 atcttttcct actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc
    33301 acctattcta ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata
    33361 catgtctttt gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc
    33421 agtcattttt ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata
    33481 gctactgaat agtaactgaa aaccttttat tcgaacatag tgatcttttc tggtgctaaa
    33541 cgaacagact cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca
    33601 taacggtctt tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt
    33661 ggatttgttt catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta
    33721 gaatcgactt tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg
    33781 atcgcagcta attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg
    33841 ttgtagattt cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca
    33901 gaagaagtat cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct
    33961 tcctcgcttt cagcgaccac aaagcgccat ggtttcatat tgacagaaga aggtgcagtc
    34021 gtcgcttctt tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat
    34081 acgcgaacgg atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt
    34141 attaacctct ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag
    34201 tgttagaaat tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc
    34261 tttatggtcc atttcacaat gcttgatttt cttctgggct ttttctgtta gcgctacata
    34321 cacttcccgg ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac
    34381 cttcagatga cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg
    34441 tagcaatgga ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc
    34501 gatattttct tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag
    34561 gtgactaatc tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat
    34621 tgacttgtca ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa
    34681 aaaacgctct caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa
    34741 atgataaaga aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga
    34801 tagctcttct gcagtagata attttacacc gccaaaaatt tccgaaatcg ccttttgcaa
    34861 gaatcccgtc gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg
    34921 acaagatgtt gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta
    34981 ataagttcaa ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt
    35041 gatgcagggc agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata
    35101 tctccagccc atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt
    35161 accagttttt tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa
    35221 acttagacct tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat
    35281 tgtgtaatga tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt
    35341 tggtgcttgc caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc
    35401 tccggcattc aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg
    35461 agaagttgaa gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt
    35521 caatgtagaa ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc
    35581 tttgtaagag ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa
    35641 agaagtcgtc ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt
    35701 tctaaaagcg gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt
    35761 aacagattgg ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc
    35821 caaagtactg ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata
    35881 taagtcattg gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg
    35941 ttgagaatta gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga
    36001 caaatctgct tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc
    36061 ttctgcattc tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg
    36121 ttctgttgtt tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga
    36181 ctgcaaagta ctttctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt
    36241 tgcgacagtt tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc
    36301 taaaagaata acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat
    36361 gaaacttttc ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt
    36421 aaataacatt tgtttctttt taataaattt gctttttctg cataaaaaga cgtaaatgaa
    36481 gaatattctt ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt
    36541 aattctctct ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc
    36601 atgttgctga cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata
    36661 tcttcagatg ttaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg
    36721 tcgctgatgg tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg
    36781 atgttatttt gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt
    36841 gttccgcaat aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa
    36901 atgatatgcc cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac
    36961 acacccatga tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat
    37021 ggagcggttg gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc
    37081 atcgctaaat cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata
    37141 tatagctcag cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca
    37201 gctataacta gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg
    37261 cttgaagcgc ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc
    37321 tgttttgaaa aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata
    37381 atccgttcga caggtttgac gattttggca gggattcctg caacgatggt attgtctggg
    37441 acatctttcg taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct
    37501 ggcagaatag ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact
    37561 gacaagcctc ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga
    37621 ggaccaatca atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga
    37681 cgattgataa agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc
    37741 agcagaacag tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg
    37801 atatctgctg gttcatggta cccagtattc agcgctgcta aaggctcttc gttttccgct
    37861 tggatctgat ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt
    37921 tgtttttcga tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg
    37981 gcttatgatg tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg
    38041 gtatgctttt ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta
    38101 gcagtagcta gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct
    38161 actctctcaa aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcga
    38221 ggctcccgag agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa
    38281 atcttgtcgt tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga
    38341 gaaatctaca ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat
    38401 tcgttgatca aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat
    38461 atcatagaca aactggatag cggattactt gattttggac tcgtaatcga accgacaaac
    38521 aaacaaaaat accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat
    38581 cggaatcatc ctttagcaaa gcaaacagtc atccatccgc aggaactgaa aactgttcct
    38641 ttgatcattt cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc
    38701 atagatcagt tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc
    38761 gataacggga cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa
    38821 aaagtattca tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa
    38881 gaccagcctc tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt
    38941 taagtagagt ttttttcctt cgctttcagc tttttaacaa cgaataagtt aagcaaaaga
    39001 tccaatgatt aaataacata agtttcttaa tttgttcctt ccttttattt ctgataattc
    39061 cagtaaatta ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt
    39121 gattacgtta acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct
    39181 gaggaacatg gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact
    39241 ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga
    39301 tctttaatca aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa
    39361 aagggattgg atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga
    39421 tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg
    39481 aacgcttttt atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa
    39541 agttgaatga acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca
    39601 ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg
    39661 atgaattcag tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat
    39721 atacttatga cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga
    39781 acaataacca tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg
    39841 aatcagaatt ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg
    39901 tgaatcattt acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca
    39961 cggaaatcgt agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa
    40021 tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact
    40081 tggccatcca tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg
    40141 aggaggaaaa aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt
    40201 tgcggtctta cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag
    40261 taacgacatt gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc
    40321 tttgctgttt gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc
    40381 cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc
    40441 tactttattg aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt
    40501 atttattggt attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc
    40561 aaaattaccg atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc
    40621 ctttgtcatg ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc
    40681 tttagtttct tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg
    40741 cttcttcaac cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg
    40801 gtttgacgta gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg
    40861 cgtaacagga atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg
    40921 agcatatgct atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat
    40981 gttagcagca gcttttgctt cattctttac tggtgtaaca gaaccacttg aattctcatt
    41041 tatgttcgtt gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc
    41101 agtgagtgca ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta
    41161 cttcctatct ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt
    41221 agtaacagca gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat
    41281 gactcctggc cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga
    41341 caataaatat gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt
    41401 aacttcgatc gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat
    41461 cgatcaaaat aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa
    41521 tgcgcaagtc atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca
    41581 tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc
    41641 cggtggaacg atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac
    41701 tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc
    41761 agaaaatgga gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa
    41821 acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa
    41881 cactgttgac ttaaaaggcg aaccattcac tttatatgta aaagaaggtc aaaaagtcgc
    41941 aagaggtcaa ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac
    42001 ggatatggtc gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc
    42061 acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt
    42121 cttccccaga cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc
    42181 tcctttttcc gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagccaa
    42241 acgtaacata tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc
    42301 ttttttacaa cctctgataa acggtgttca aaaaccagtt tatcggtatg aagtcaaatt
    42361 tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tcatactcaa aactaatcgg
    42421 ctttttcaca acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca
    42481 agagaagtta atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga
    42541 tcctttttaa tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg
    42601 gcttctttta gattttcgac aaacgctgca tcacttgctt caataatttc taacttttta
    42661 cctgtcgcac gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat
    42721 tctaagattt ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca
    42781 cgtttcaatg cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt
    42841 aaagcctgcc caatgatagg taattcattc tctacatacc aattattccg taaaaatgta
    42901 tgggaaatac ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaataca
    42961 ctgatcgagt gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct
    43021 tgtgctgcct tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca
    43081 aaaagcaatc gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca
    43141 ccaatacgta tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact
    43201 agtacataga tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca
    43261 aaacctccag ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat
    43321 ttgttaagat ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa
    43381 gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag
    43441 tgatgcaatc catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag
    43501 tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca
    43561 gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa
    43621 aagtccagat cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct
    43681 tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc
    43741 tttagaagaa agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac
    43801 acttgatagt ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac
    43861 aggcctcgta gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa
    43921 tcgtctacat aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc
    43981 tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta
    44041 gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc
    44101 ctctctaatg ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg
    44161 actaccggta tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca
    44221 gcatttggac ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc
    44281 gtattcgtca aatcatttct taattcttca ggcgattctg ctaaagcgat tacaacaaga
    44341 tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg gaacatcctg actcgtttga
    44401 acaccgacat agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga
    44461 ttttcagcag cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat
    44521 gcttgtttct ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca
    44581 atacccgtca ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc
    44641 ctttctatta tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc
    44701 ttttttctag ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc
    44761 tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg
    44821 actaactcat aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt
    44881 aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca
    44941 agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc
    45001 aaactgccct tttcttccaa gcgggctcca tcaaatccat aagaaacgtt gattacttga
    45061 tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc
    45121 gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca
    45181 cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg
    45241 acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct gctgatttct
    45301 gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat
    45361 ccatatctaa acgccgtttc tgtcactctt gatccatcta ggagatcgcg attagccaaa
    45421 gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg
    45481 cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct
    45541 tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc
    45601 ttcctccttg cttgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag
    45661 gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc
    45721 gtttcgtaac ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa
    45781 ttttggggaa gacacattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt
    45841 taatccgctt actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc
    45901 tgcttggatt tttctttgcg atacatcata cctcttttat caattggaac aattatttat
    45961 ttctaacaaa atcacttcat ttttgtcaga taaataaaaa cgtaaatttc aagtttaagt
    46021 tagccactac aggtaactca tttgtttgat accgtaactg cctcgttatt ctaaatcagt
    46081 gaggccttga taatgaactg aggtatgata gtcttcagac gattctggcc ccaggccagc
    46141 tttccagaca tgaagccatg tcccgagtga ggatcgttat caaggaataa tgggtcaagc
    46201 caccaaccgt tcttgcttga atgcctggac gaatgtatca tgtggtgtcg cataacccaa
    46261 aattttccgc gggtaatcgt tcatccaacg ctggatccgc aaaatctggt tctcgctcac
    46321 ctcactaatc gggcttccct tggggacgaa acggcgaatt aatttgtgct ggttttcgct
    46381 cgtcccacgc tcccaagaag cataggggtg gctgaaatag acgtcagcta catcttgcag
    46441 agcatcgtgt agcccagaaa actccgagcc attgtcggat gtaacggtct taaataattg
    46501 atcgaagtta gcgccagccc gttctcttag ctgttgaacc gcttggtcca ccgattcggt
    46561 gtccttcccg ttcaacttta ggatgacttc aaagcgtgtc tgccgttcag ccagcgtcag
    46621 cagcacggca tccgacttgg tcttgtttcc cactaccgtg tcgatttccc aatgaccgaa
    46681 cgtttcccgt gtttcaactt ctttagggcg ttcttcaatt gacgtcccga gaacacgctt
    46741 gttgggacgg tgtcctgggc ggaccgcttt gattttacgg gacagttttt caagcaagtc
    46801 catgttcttt gttcgcatga ttccgcggtc aatccattga taaagagcgg ttgtgccagg
    46861 aatgatggca caatcgaaca gctcgtgttt tctggcaaat ccgaccacga cgtccggcga
    46921 ccatttttca tttaacattt tgtcatctgc ccaatcaatg aaggcatttg tgttcgccca
    46981 tttggggcgt ctgccacagt tcagtcgttg cttttcatag aaggcctgtc ctgcatcagc
    47041 gtcataaaca gaatagtagg tgtcatacac ttttccattt tgcgtttggc gttttaactg
    47101 ggtgatggtt ccgcgatcaa tctcattgtt tatggtttgc ggaacacgtc ccagcacttt
    47161 ggcaacctga cggttggaat agccttcctt tttcaggata gcgatttggc aacgttctga
    47221 gtaggaaaga tgttttccct tgcgtgaaac tgtgttatca tgggtttgcg tcatgtgaat
    47281 tca
//
LOCUS       DACDBG010000017        43213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDBG010000017 DACDBG010000000
VERSION     DACDBG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.017"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            175..627
                     /locus_tag="IVZ90_000890"
     CDS             175..627
                     /locus_tag="IVZ90_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8261958.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(751..1602)
                     /locus_tag="IVZ90_000891"
     CDS             complement(751..1602)
                     /locus_tag="IVZ90_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8261959.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1615..2400)
                     /locus_tag="IVZ90_000892"
     CDS             complement(1615..2400)
                     /locus_tag="IVZ90_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8261960.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2752..3693)
                     /gene="ribF"
                     /locus_tag="IVZ90_000893"
     CDS             complement(2752..3693)
                     /gene="ribF"
                     /locus_tag="IVZ90_000893"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8261961.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3697..4620)
                     /gene="truB"
                     /locus_tag="IVZ90_000894"
     CDS             complement(3697..4620)
                     /gene="truB"
                     /locus_tag="IVZ90_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8261962.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4988..7350)
                     /locus_tag="IVZ90_000895"
                     /pseudo
     CDS             complement(4988..7350)
                     /locus_tag="IVZ90_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            7798..7971
                     /locus_tag="IVZ90_000896"
     CDS             7798..7971
                     /locus_tag="IVZ90_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8261963.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(8128..8475)
                     /gene="rbfA"
                     /locus_tag="IVZ90_000897"
     CDS             complement(8128..8475)
                     /gene="rbfA"
                     /locus_tag="IVZ90_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8261964.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8502..10808)
                     /gene="infB"
                     /locus_tag="IVZ90_000898"
     CDS             complement(8502..10808)
                     /gene="infB"
                     /locus_tag="IVZ90_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8261965.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10821..11132)
                     /locus_tag="IVZ90_000899"
     CDS             complement(10821..11132)
                     /locus_tag="IVZ90_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8261966.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEIAQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11129..11422)
                     /locus_tag="IVZ90_000900"
     CDS             complement(11129..11422)
                     /locus_tag="IVZ90_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8261967.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11444..12619)
                     /gene="nusA"
                     /locus_tag="IVZ90_000901"
     CDS             complement(11444..12619)
                     /gene="nusA"
                     /locus_tag="IVZ90_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8261968.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12642..13115)
                     /gene="rimP"
                     /locus_tag="IVZ90_000902"
     CDS             complement(12642..13115)
                     /gene="rimP"
                     /locus_tag="IVZ90_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8261969.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13260..17618)
                     /locus_tag="IVZ90_000903"
     CDS             complement(13260..17618)
                     /locus_tag="IVZ90_000903"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8261970.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17830..19539)
                     /locus_tag="IVZ90_000904"
     CDS             complement(17830..19539)
                     /locus_tag="IVZ90_000904"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8261971.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19607..20875)
                     /gene="rseP"
                     /locus_tag="IVZ90_000905"
     CDS             complement(19607..20875)
                     /gene="rseP"
                     /locus_tag="IVZ90_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8261972.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(21036..21836)
                     /locus_tag="IVZ90_000906"
     CDS             complement(21036..21836)
                     /locus_tag="IVZ90_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8261973.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21833..22645)
                     /locus_tag="IVZ90_000907"
     CDS             complement(21833..22645)
                     /locus_tag="IVZ90_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8261974.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22840..23397)
                     /gene="frr"
                     /locus_tag="IVZ90_000908"
     CDS             complement(22840..23397)
                     /gene="frr"
                     /locus_tag="IVZ90_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8261975.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23400..24122)
                     /locus_tag="IVZ90_000909"
     CDS             complement(23400..24122)
                     /locus_tag="IVZ90_000909"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8261976.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24258..25139)
                     /locus_tag="IVZ90_000910"
     CDS             complement(24258..25139)
                     /locus_tag="IVZ90_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8261977.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25238..26020)
                     /gene="rpsB"
                     /locus_tag="IVZ90_000911"
     CDS             complement(25238..26020)
                     /gene="rpsB"
                     /locus_tag="IVZ90_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8261978.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26379..26858
                     /locus_tag="IVZ90_000912"
     CDS             26379..26858
                     /locus_tag="IVZ90_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8261979.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27075..28166
                     /gene="phnW"
                     /locus_tag="IVZ90_000913"
     CDS             27075..28166
                     /gene="phnW"
                     /locus_tag="IVZ90_000913"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8261980.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28159..28287
                     /locus_tag="IVZ90_000914"
     CDS             28159..28287
                     /locus_tag="IVZ90_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8261981.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28291..28836
                     /locus_tag="IVZ90_000915"
     CDS             28291..28836
                     /locus_tag="IVZ90_000915"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8261982.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29290..30981)
                     /locus_tag="IVZ90_000916"
     CDS             complement(29290..30981)
                     /locus_tag="IVZ90_000916"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8261983.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31401..32348)
                     /gene="arcC"
                     /locus_tag="IVZ90_000917"
     CDS             complement(31401..32348)
                     /gene="arcC"
                     /locus_tag="IVZ90_000917"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8261984.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32463..33482)
                     /gene="argF"
                     /locus_tag="IVZ90_000918"
     CDS             complement(32463..33482)
                     /gene="argF"
                     /locus_tag="IVZ90_000918"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8261985.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33573..34802)
                     /gene="arcA"
                     /locus_tag="IVZ90_000919"
     CDS             complement(33573..34802)
                     /gene="arcA"
                     /locus_tag="IVZ90_000919"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8261986.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            35263..35964
                     /locus_tag="IVZ90_000920"
     CDS             35263..35964
                     /locus_tag="IVZ90_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8261987.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(36573..37589)
                     /gene="tsaD"
                     /locus_tag="IVZ90_000921"
     CDS             complement(36573..37589)
                     /gene="tsaD"
                     /locus_tag="IVZ90_000921"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8261988.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(37586..38050)
                     /gene="rimI"
                     /locus_tag="IVZ90_000922"
     CDS             complement(37586..38050)
                     /gene="rimI"
                     /locus_tag="IVZ90_000922"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8261989.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(38057..38599)
                     /gene="rimI"
                     /locus_tag="IVZ90_000923"
     CDS             complement(38057..38599)
                     /gene="rimI"
                     /locus_tag="IVZ90_000923"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8261990.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(38583..39407)
                     /gene="tsaB"
                     /locus_tag="IVZ90_000924"
     CDS             complement(38583..39407)
                     /gene="tsaB"
                     /locus_tag="IVZ90_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8261991.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(39496..40476)
                     /locus_tag="IVZ90_000925"
     CDS             complement(39496..40476)
                     /locus_tag="IVZ90_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8261992.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(40500..41984)
                     /gene="galT"
                     /locus_tag="IVZ90_000926"
     CDS             complement(40500..41984)
                     /gene="galT"
                     /locus_tag="IVZ90_000926"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8261993.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(41996..42985)
                     /gene="galE"
                     /locus_tag="IVZ90_000927"
     CDS             complement(41996..42985)
                     /gene="galE"
                     /locus_tag="IVZ90_000927"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8261994.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT        11788 a         9232 c         7597 g        14596 t
ORIGIN      
        1 tatatttaaa tataatatca ctttaggaga gaacgaattt ctttagagaa ataaaagtta
       61 gaattttctg acttgatttt tacaatgacc ttaattaaaa tcgttctttc tacatttcgt
      121 gattaagcaa atatcaacta agtcagtaga accattcaca taggagggtg aacattgaaa
      181 gatttttact cagtaaatga attagccgaa caattaggtg ttactacacg ttctatcaga
      241 aattatcttc atgaaggaaa actaaaagga acaaaggttg gcgggcaatg gaaattttct
      301 gaacgtaatc tttttgagtt cctatacgga gatcaagcag aggaagcagc aaaagaaatg
      361 cagcgattca tgctaaatgc accaatcacg atgcgtttca accttcaata tcgtgatttt
      421 acagcaatca atcaattccg agaacaatta gtccagtacc ataacgatgt ttacgcaaat
      481 aaaaaagacc gtcttttaca atacgatctt tataaagaca atcatgcgga gatactaatc
      541 ggtggtaact tcaattatgt cgtaaacttt agtcaatgga tcaatgaaaa attattgatc
      601 cagacagata tttcccttgt atcttaaaca attaaaaagc gctagaaatt ccagagatgt
      661 tggaaattct agcgctattt ttttataagt acttactgtt tggttcaaac aaatattttt
      721 ttaataaatg aaattttacc atctttatcc tcaaataaag accgtgacca tgcttcctac
      781 tacgatcagc aataatccga taatcgttaa ttttttttct tttggtgtct tattttcatg
      841 caggatgtac ataccgccta atgtagagat aactacgttt agctgtgtaa tgatatatgc
      901 agtagcaacc ccattttcat ttgctgaaaa aatataagcc aatgctgaca cactaaagat
      961 ggctccaacg atcgttgctt tccaactctt gtcttcagaa aatgcggttg gtttacggaa
     1021 aatcacataa atcactgccc ctaaaaagac accgatcatt tgcggaaaga aaatggaaag
     1081 tccgcttgca tcaactagtt taggcaatgc actgtatacc caataaccaa tacttgttga
     1141 tgctaataga ctaattcctt ttgtgatacc gccttttttt ccaggatcat cactgattgc
     1201 cgttaaagca gaaccaaata caagaagaat gattgctgct gctccaatga ttttccccat
     1261 acttccagac cattctccaa aagcgaagac accaatcaac gaggttccga ctaactgtaa
     1321 tgcagtcgaa attggcatcg ttttagaaac accaatcaag ttgaaagatg tatactgtcc
     1381 tgtttgtcca ataacccaaa atgcaccaga aagtaaacta atcaaaaatg tagtcactga
     1441 aataccagct ggagaagcga ataattgaac gagtaagccg acaagcaccg ctccaatacc
     1501 agttcctaat atctcatttg ttggtctgcc accaatcttt ttcaaaatca gtggctggat
     1561 cccccagcca actgccggga tcaatgcaat caataaaccc atttttattc acccttatcc
     1621 ttttccacct tggaaagaag gatataaagt catcccacca tctgcaaata gtgtaatacc
     1681 tgtaacataa ctggattcgt ctgaagccaa ccatgcagct gtcgccgcta tttcttttgg
     1741 ttcacctacg cgtcctagtg ggatcatact ttccaagagt tctttttgtt caggatcatc
     1801 aaattttgcc gcattaatag gtgtacggat agcaccaggg cctatactat tgattctgat
     1861 tcccttcggt gcatactcca ttgctaccgt ttcggtaaat aattttaccc cgccttttga
     1921 agcagcgtaa tgagcaaacg ttggccatgg gatctgttca tgaacagaag acatattgat
     1981 gatatttcct tttttattat tctctgtaaa gtaatttaaa gccgctttcg ttccgataaa
     2041 aacgccagtc aaattgactt ttaacactct ttcccaatct tcaaggctaa gttcatgggt
     2101 tggatgctga ttttccatcc cagcattatt aacccaaacg tccagtgtcc caaaattttc
     2161 taaagccgta tcaagtaaac gctgtattcc ttcttcagaa ctgacatctg cttgtacaga
     2221 gactgctttt ccgcctgcct cttcgattgc tttgactgct ttttctgctc cttctttatc
     2281 cgagttatag ttgacaacta cattcatttt ttctttcccg aatcgttgag caatcgctgt
     2341 tccaatcccc tttgagccac cagttactac tgcgactttg ttttctaatt ccttgtacat
     2401 agtacccact cctttttcat acctaaacct gttattacgg ataactttta tttccgcctt
     2461 aaagtgagtg tacaccaaga tatatagtct aaagcaaatg atacgctcta gcaatcgttt
     2521 tcatgaattg ctagtgtcct tttatttcta taaagaaaga ggttgtgaca gaagtggtca
     2581 gcttcaagaa atttcggctt atttccgaag aagctacttc tgaaaaacca tttatttgga
     2641 aaaaaaggct gtgacaagac ttttgtcaca gcccctttcc tgggcttatt tatcacccat
     2701 tttattgtaa ttgctagtta tacataaaca agagatttta tgcataatgt tttattgctg
     2761 agaaaaatat tcagctgttg cttgttcatc ctccttgagt tgttcaatca attcctctac
     2821 actagaaaat ttcacttgat ctcttaaaaa ttgattccag cgtaccgcga catgttcacc
     2881 atagatatct tgattaaagt ccaaaatata caactctaca gtcagctccc gtccttcacc
     2941 gaatgtatca ttatgtccaa ttgaacccat ggctggatac catgtttccc caatttttat
     3001 ttccgacaca taaacacctt ctttaggcaa atgaaccgtg ctctttactt tgacatttgc
     3061 tgtagggaat cctagtagtc tgcctcgtgc atcgccatgg acaactaccc cttctacctc
     3121 atatgtgcaa cctaatagtt ttgctgcttc ttttacttgt ccgctgtcta gtaatccacg
     3181 aatacgggta gagctgattt tttcaccttg ttcttcttct ttagaaactg tgacgatttt
     3241 gaatcttcct ttggcatatt caggaaaatg agcgacatct gcaatgtctt ttggtccata
     3301 cgtataatca aaaccggaaa cagcatattt tgcatgtaac cctacgatgt attgatcaac
     3361 aaattcctgc ggtgctaaat gagcaaaaga agaagtaaaa tcaacttcat ataaataatc
     3421 tatacccaaa gcggccattt tttcttcttt ttgcttaagg ctagtcaaat atttcacgct
     3481 gctaggatcg atctttttaa agacaataga tggatgctga ttgaaagtca tcaatgctaa
     3541 tttcaatcct tctttttgtg caatttccat acctgtcttt atgactttct ggtgtccgat
     3601 atgcacgccg tcgaaaaatc ccagcactaa aacgacatcg tcttctggaa tttgttctga
     3661 ttggtaagga tgtctgatct tgatgatatt cattcgttat tcctcatttc tcaatacttt
     3721 actaggtttt aatttgtttt tttctttcgg gtttggctga taaatactga caacttttcc
     3781 tttataaaac aaagcaattt cttctgacgg catttctggg agcgcaaata cagtataatc
     3841 aagtctcata ccgtttttta ctttctgcca tacttcatca gtaatatcta ctcgtttgaa
     3901 ccgttccact cctttttcta ttggcagcaa acaatcatcg attgtcccat ttttcacagc
     3961 ttgagctact tcttctaatg tcagagcatc cttctgggac ataccggcac ttgctgttcg
     4021 ggtcagatca gacatgtgcg cagggtaacc aagtttcttg cctgtatcta cagctaatgt
     4081 gcggacatag gttcctttcc cgcattctac ttcgaagcgc caagaaatgg ttcctgtcac
     4141 ttcgtctctt gtcaattcac ttgtccggat aaaacggtaa atctgagcct ttctaacagg
     4201 acgctccact gtttcattat tgcgggcata ttcatataac cgcttaccgt tgacttttac
     4261 cgctgagtac attggtggta tctgggtgat ctcaccaata aatgaagcca tcacttcatc
     4321 tatttttttt tcagataaag acatatctac tggttttgtt tctacccgtt ctccggatcg
     4381 atcttccgtc gttgtactga aacctagagt tatctcgcct acatagattt tcccagaatc
     4441 tgtcaaatat tcgataactt ttgtggcttt tccaatacaa atgggcaaca caccgtctac
     4501 gtctgggtca agcgtacccc catgtccgat ttttttcgta tgtaagattt ttcttaattt
     4561 gaatacgcaa tcatgacttg tcattccgcg ttctttccat aaaggtaata acccgtccat
     4621 gtcttttctc catttcattt ttgatttaac tgaattatta taacacattg catcaattac
     4681 tgacgaattt gacatcataa tgaacggttt ttatttttcg gcaataaaaa cgtaacaaaa
     4741 ttcatgctca cgtaaataga aatcatgtta tgctttttta aaggagggaa tagaatggaa
     4801 gattttttct ttgcaccatt tgattggatt tatgggtggc ttacaggtaa atagtttggt
     4861 tttcacttcc caaaaacgaa aaaaaagact ggccattctt ccagtctttt ttccgttttt
     4921 tttatttatt ttttcagttt cttaatcaat gtaaaaaatt ctattttgcg ggatcgattt
     4981 tatcatttta ttggtcaaga tttttccatt tccaatccaa tacttctggc agatcaatac
     5041 cattttcacg aatatagttt tgatgttctt gtaatttctg atccattttt ttcgcaaaat
     5101 cttctgcttg acttcctttg atttttaaag ctgcgtcttt tgccaaatga taacgatcaa
     5161 gttcatttac tacacgcatg tcaaatggtg tcgtgatatc tccattttct ttgtagccat
     5221 gaatcattaa atgatggtta tgacgatcaa agaaaatcgt gcggaccaat gtttcataac
     5281 cgtggaaagc aaataagatc ggtttgtctt ttgtaaaata ttgatcaaat tcttcatcgc
     5341 tcaacccacg cggatcttga cttggatgac gtaatttcaa taaatctacg acattgatga
     5401 accgaatttt tagttttggg aattcttcca gcagcaaagt tacagctgct aatgcttcta
     5461 agttagattc cgttcctgca gcagcaatga ccaattcagg ttcctcctct tttgctgtcg
     5521 aagcccaatc aatgattttc aagccttcat tcactaagac ggctgcttct tctgctgagt
     5581 aaaattgagc acgcggatgt ttggaagaga tgatcaaatt gattttttct gacgagcgga
     5641 atgctttatc catgactgcg agcaatgtat ttgcatccgc aggcagatat tcacggatga
     5701 actccgcctt cttttctgcc aaatgtgtca atattcctgg atcttggtgg ctatatccat
     5761 tgtgatcttg ctggaagact gttgagcttg caatgagatt tagcgaagga taatcatttc
     5821 gccaagaaag gtcatgtgat ttccgcatcc atttgaagtg ctgagtcaac atagagtcaa
     5881 ccactcgcaa gaatgattcg taacttgcaa aaaaccatgc cgtccagtca gaacgtaccc
     5941 ttctaagaac ccttcatctt ggtgttccga taattgactg tcgatgacgc gaccagatgg
     6001 cgaaagccat tcatcatttt ctggttcgat tttttccatc cattggcgat ttgtcgtttt
     6061 gaaaacttga ttcaatcggt ttgattttgt ttcatctggt ccaaatatac ggaaattttc
     6121 tggatttttc tcaatgatct cgcgaacgaa tttaccaagt tcggtcatat cttctgcttt
     6181 aacggagcct ggtttttcaa attgcaaagt atattttttc caatctggca taataagcgg
     6241 tcttggatcg attccaccat tggtgatagg attttttgcc attctttggt caccagtagg
     6301 cgcgatttct ctgatttctt ctaaaatacg tccttgtgca tcaaataatt tttctggttc
     6361 ataggatttc agccaacgga gtaaagcatc tgcatgatcc atatgttctt gatctacagg
     6421 aatcggtact tggtgtgctc ggaaggttcc ttcaattggt tccccgtccc attcttttgg
     6481 accggtccat ccttttggtg tgcgtacgat caatactggc cattttggca tcacgacatc
     6541 tgttgctgtc ttcaggcgag cttctttttg gatggattta attttctcga ttgcttgatc
     6601 cattttttca gccataacag gatttaattt ttctgaatca tttccttcaa taaagaaagg
     6661 ttcccagcca agtccgttaa agtaatttgc taattctcca tctgatttac gtgccaagat
     6721 cgttgggttg gctatttttg caccatttaa atgaaggatc ggtaaaacag ctccatctat
     6781 cactggattg ataaagacat ttgagaacca gcttccagct aaaggaccgg tttctgcttc
     6841 accatcacca atcacgacag cagagattac ttcaggattg tctaatacag ctccgactgc
     6901 atgtgaaaga gagtatccaa gctctccgcc ttcatggata gaacctggtg tttctggtgc
     6961 tgcatgagaa gcaatgcctc caggaaatga aaatcgtttg aataattttt gcattcctgt
     7021 ctcatcttcc gttacttctg gatagatctc tgtgtaactt ccatctaaat aagcattcga
     7081 taccatgact tgtccgccgt gtcctggccc ttcaatataa aacatgttca aatcatattt
     7141 attgatgact cggttcaaat ggacataaat aaaattttgg ccaggaatcg ttccccaatg
     7201 accgattgga tgttttttga catcttcggg tttcagtgta cgtctcagta aggggttatc
     7261 tttaagataa agttgcccaa cagaaagata attagcagca cgccaccaag ccgtcatttt
     7321 atcaaagtat tctttactag aataatccat acataacact ccttttcatt attactctta
     7381 ttctagcgca gatactccca attgaaacct aataagcact tctgttcatt tgtttggatt
     7441 ttatatctag ccaaatgaac aggtataaaa aaccacgaaa tttttccttt cgctgacgtt
     7501 ttttacattg atccaaaaaa cttgtcgtta tcacaatggc tgttccgcag ttcatttcgc
     7561 tcctcatcat gcataatttg ctgaatgacg ctgggccagt taacacattg ctggaaaact
     7621 ggggctggat cgagcaattc ctttcctgat tgatccgttt tatgtaaaag tctctgttat
     7681 ctttgtcaat atgtgaattg gtatcccagc tactatgcta atttctacag ggattatcca
     7741 aaatttggat cgtgaccaaa tcgaagcggc gtaatcggaa atatcaataa cttcaacgtg
     7801 atttttcttt taactgtcaa cggaccgaca aactcaaact tttatggtgc aggttccact
     7861 gatcttctag ttacttggct atacctgact gtcactaaaa tagattacaa tctagcttcc
     7921 gttatcggta tcttgatctt tatctatatg caaaaattta tatactacta gaaaaaagaa
     7981 agtcgaattt tttacttttc tttttctctt ttttggttag ctgacacgcc gcaagactaa
     8041 agtcatggaa ctcttaggta atacgtctta atgaaaacaa aaaaatcccg caacccctga
     8101 tttcacagga tttgcgggag tttttgatta gtctttgttc aagtcgcgaa gcaattcatc
     8161 gatatggttg ccatagacaa ctgattcatc aagttcaaag aacaattcag gtgtcttata
     8221 aatacttaga ttgtgcccta actctcgacg aatcaatcct gatgctttat tcaatccatc
     8281 ttgtgctgtt ttcttatctg atgctaagtc agataaaatg ctgtaataga tcgttgcttg
     8341 ctgtaagtcc cccgtaactt tcacatctgt gatcgttacg ttctccacac gagggtcacg
     8401 gacctttttg cgcaaaatat cattgacttc ttttaagatt tcttgaccga ctcggcggtc
     8461 acggtagtta gccataatga ttggcctcct tttatcagtt tttagtcatt tttgacttct
     8521 tccatgataa atccttcaat tacgtcatct actttgatgt cattgaattt ttcaatcatt
     8581 gcaccacatt cataacccat tttgacttct ttcacgtcat ctttgaatcg tttcagactt
     8641 gcaagttggc cttcatagat tacgattccg tcacggatta cacggacacc gctatctcta
     8701 cgaatatatc cttctgttac ataggcacct gcaatcgtac caactttaga aactttgaag
     8761 gtttcacgaa cgatcatttg accggtgatt ttttcttcaa attcagggtc tagcatccct
     8821 ttcatcgcag tttcgatttc ttcgatggct ttatagatga tacggtgaag acgaatatcg
     8881 acttcttcag tatcagcttg cacttttgcc tgcggtgtag gacgaacgtt gaaaccgatg
     8941 ataatagcgt tgctggctgc tgctaaggtt acatcacttt cattgattgc tccgacagct
     9001 gaatggacga tttttacacg gacaccttcc acatcaatct tcttcaatga tgcagccagt
     9061 gcttctgctg aaccttgtac atctgctttg atgattacgt taacttcttt caattcgcct
     9121 tctttcaggc tttcaaataa gttatccaaa gtcacacggc ttgtggcagc acggttttct
     9181 aataatgcac gtttcgcgcg ttcttcaccc gctgcacgtg ctgtcttttc gtcttcgaag
     9241 actacgaaac ggtcacctgc ttgaggtaca tcattcaagc cagtgatttc tactggcgtt
     9301 gcaggtcctg cttctttatc gcgacggcca agatcgtttg tcattacacg gacacgtccg
     9361 taagtattac cgacaacgat tgggtcacca acatgaagtg ttccttcttg gacaagtaag
     9421 gttgacacag gacctttacc tttatcaaga cgtgcttcga tcactgttcc gattgcgcgt
     9481 tgtgttggat ctgcttttaa atcttctact tctgcgacta acaggatcat ttccagcaat
     9541 tcttcgatgt tttggccgaa tttcgctgag atctcaacaa agatcgtttc tccaccccat
     9601 gcttcaggaa ttaactcata ctcacttaat tcttgcataa catgttgagg gtttgcacct
     9661 ggtttatcaa ttttatttac agcgacgatg attggaacgc ctgcagcttt ggcatggttg
     9721 attgcttcaa ctgtttgtgg catgacgcca tcatctgcag caacaactaa gatcgtgata
     9781 tctgtgatac ttgctccacg tgcacgcata cttgtaaagg ccgcatgtcc tggtgtatcc
     9841 aagaaagtga ttggtttacc atcaatatcg atctggtagg caccgatatg ctgtgtgatt
     9901 ccgccggctt cgccgcttgt tacacgtgaa tggcgtaatg tatccaataa agttgttttc
     9961 ccatggtcta cgtgtcccat aatcgttacg actggcggac gtgagactaa tttatctgga
    10021 tcgacttctt ctgcctcgaa gaatttatcg atatcagcaa tgtcaacttg gactttttct
    10081 tgtggttcca tgccatagtc cgtcgctaac agttcaatcg tatctttatc cagtgcttgg
    10141 ttttgattga ccatgacacc aagcataaat aattttttaa tgatttctgc tggctcacga
    10201 tgaatttttt tcgcaatatc tgctacgttc ataccttcag tatactctaa tacatctggc
    10261 aattcacgga atttgcgtgg tggtacagct ggtttagttg attgttgctg tttgcctttt
    10321 ttaccttttt tattataacg attacggtta ttattattaa atttgccgcg attgttattg
    10381 tttggtttgt tgttattgtt agtacggttt tgattgccgc cttggctcgt tccttgacga
    10441 ttttggctgc tggtatttcc cgtattattt tggtttgttg ttggtttttt tccttgattc
    10501 acttgaccgc tcccttgacc gcgatcttga taactgcggt tatttttatt tttttgttga
    10561 ttgttcgttg tttctttttt acgttgatcg ttcggctgtt gattggctgg ttttctgctt
    10621 gcttgttgtg cagcttgttg tttcgtgctt gggtttttga acgcttgttg caatttcttc
    10681 tcatcattgc tagtaatcgc acccatatgg tttttcacat cgatgccaag ttgttgtgct
    10741 ttttccacga cgtctttact agattttttg agttcttttg ctaattcata gattcttttg
    10801 ttgcccatgc aatcaccttc ctaaccttca attagctcct tggtcttctt cgcaaatccg
    10861 gcatccatta ttccgaatac cttgcgaggt tttccgatca tctgcgcgat ttctgcttca
    10921 gaaaatagct caaagcaagg aacttcataa tatgaactct tatcttttat cttttttctt
    10981 gtattctcgc ttgcatcggt tgccacaaac accaattttg ctttttgttt gcggatttca
    11041 gcaatggtca gttcttcacc cgtgattagt tttcccgcgc gcatcgctag accgatgaga
    11101 ttcatggctt tttgccgatt aactgtattc attttccaaa taactcttta cgtgcttttt
    11161 ggtgagtgac atagtcaaga agttcttgat aaaactcatc tgttaatgtt gtttcgagta
    11221 ctcgatcgag aattttttta tcccaagctt gttgtacttc ttccggctca agtgagacat
    11281 acgctcctcg tccaggcatt tttccagttg gatcgatcga tacttcccct tcttttgaac
    11341 gggtgattcg gaccatctct tttttaggtt tcatttcacc tgaaaccacg gatttgcgta
    11401 aaggaatttt cctttgtttc atctctttgc ctccttacta aagttaagct tctggttttt
    11461 cttcgaccgt ttcattgttg aatgcaattg tttcatattc atcgtctgtt aaatctgact
    11521 gaacgattgc ttcatcatgc agttcttctg tttctttttg ttgttcttga ttttcataaa
    11581 attccgtcat gtcagattct gatttgatat cgattttatg gttagtcaat ttagcagcta
    11641 aacgtgcgtt ttgtccgcgt ttaccgattg ctagcgataa ttggtagtct ggaacgacaa
    11701 ctgtgcaggc ctttggattt gtctcatcga agatcacatc catgacttgt gcaggattca
    11761 atgcatttgc aatgaataca gctggatctt cattccattc gacgatatcc atgttttcgc
    11821 cttttaattc attgacgatt gcttgaactc gttgtccctt tggcccgaca catgtaccga
    11881 ctgcatcgat atttggatca tttgagcgaa ctgccacttt tgaacgatcg ccagcttcac
    11941 gggcgatgct gacaatttca acgattccat catagacttc tggtacttct tgctcaaata
    12001 agcggcgcaa taggtctgga tggctgcggc taacaaatac ttgcggacct ttagacgtat
    12061 tttctactcg ggaaacataa actttgattc gatcatgtgg ttgataaaat tcatttggca
    12121 tttgatcttg tttagaaaga actgcctcaa ttttaccaag attgacataa atataacgtt
    12181 tatcctggcg ttcgacgatt ccttgcatga tgtctttttc ataagcactg aattcgttat
    12241 aaataatcgt tctttctgct tcacgtacac gctgtaaaat aacttgttta gccgtttgcg
    12301 cagcgatccg cccaaaatct tttggtgtta cttcaaaacg gatcgtatca ccgatctcat
    12361 atgctggatt gattaaaagt gcatctttca atgagacttc caactgtgaa tccatcactt
    12421 cttccgttac ttcttttacc gcatagacat ggatctttcc ttttttctga tcaaattcca
    12481 cttcgacgtt ttgtgcttgg ccatagtgac gtttgtatgc agaaaccaat gctgcttcta
    12541 aagcatcgat cacgatttct tttgagatcc ctttttcagc ttctaaagcg tctaacgcat
    12601 ttaacatttc tttactcatt ttttctgatt cctctccgtg attaaaattg gatagctaag
    12661 cgagcttttg caatattttt tcggtcaaaa gtcaattctt tttctcgtgt ttttatgcgt
    12721 attttcaatg taagctgttc agcatcaaat gattgaagaa atccttcata ttgtttttcg
    12781 ccatctactg gttggtataa ggagacatgg acataatcac caagtgcctt ttcatagtcg
    12841 ctttcttttt tcaatggccg ctctgctccc ggggaggata cctccaggaa ataagcttga
    12901 ggaatcggat ctggatctgc tgcatcaagt ttttcactca gtttctcact gacaaaggcg
    12961 cattcttcga tatcgatacc gccttctttg tcaataaata ctcgtaagaa ccagcttttt
    13021 ccttctttta caaattcgac ttcgacaagt tcaaaatttt gttcatctaa aatcggtgtc
    13081 accatttctg tgactgtttc aacgacgcta ctcaaaagtt tcgcctcctt aaaaaattaa
    13141 gtgcgtaatt ccattaaaaa gagtgagcga aaaatccgct cactcacagt tagtatcatt
    13201 acctataaga actataccat actctgctta aaattacaag cagacaggaa aacagatgct
    13261 tatagcatat cgaataacga taattgattt tcatctggta gatctttcaa tacgccattg
    13321 tcgttcatat attcgatcaa tgtttttgaa actttaccgc gggtggcaag atcttctttg
    13381 gataaaaatg gaccgtcttt tcttgcttca acgatttgtt ttgccacgtt ggttcccaaa
    13441 cttgggacag ctctaaatgg tgcgatcaac gtatcacctt caataacaaa gttaacggca
    13501 tccgatttat acaaatcaat cattccaaat ttgaatcctc gttccagcat ttcattggct
    13561 aactccagca ctgtcagttg attcttttct tttacggaag catctaatcc tttgtccgtg
    13621 atttctttca ttgcttgttt cacagcatct tttcctttgc acatggagac tagatcaaag
    13681 tcgtctgcac gtacagaaaa gtatgcacag taatacaaaa tagggaaata aaccttgaaa
    13741 taagcgacac gaagagccat caatacgtaa gctgcagcat gggctttcgg aaacatatat
    13801 ttgatttttg aacaagaatc gatataccag tcgggcacat tattttcttt catcgccgat
    13861 agataggttt ctcgcaattc atcagggatc ttgttccatt gtcctttacg aacagtttcc
    13921 atgattttaa atgccatccc gctatctaat ccagcatgga tcaaataaac cataatatca
    13981 tcacgacagc cgattacttc cgccagtgtc gcatctcctc gtcgaatcaa ttcttccgca
    14041 tttcctaacc atacgtctgt accatgagat agtccggaaa tttgcagtag ttctgcaaaa
    14101 gttgtaggat gcgtttcttc tagcatccct cgaacaaaac gagtgccgaa ttctggaata
    14161 cctaatgtcc ctgttttgga ataaatttgt tcctgactta ctccaagtac ttcaggaccg
    14221 gcaaatatcc gcatcacttc cgggtcatcc gtagggatcg tttgtggatc gatccctgat
    14281 aaatcttgaa gcatccgaat aacagttggg tcatcatgcc ctagaatatc taatttcaat
    14341 acattatcgt gaatcgagtg aaaatcaaaa tgagtcgttt tccattccga attttgatcg
    14401 tctgctggat attggatcgg cgtaaaatca tagacatcca tataatcggg aataacgatg
    14461 atcccccctg gatgctggcc ggttgtccgt ttgactccag tagcgccttt tgctaatctg
    14521 tcgacttctg cacttcgaaa ctgcagatta ttgtcccttt cgtatccttt tacatagcca
    14581 tatgctgttt tgtcggcgac tgtaccaatc gttccggcac ggtaaacata gtcttcgcca
    14641 aacaatactt tcgtatagtt atgggcttct gcttgataat cacctgagaa gttcaaatca
    14701 atatcgggca ctttgtctcc atggaatcct aaaaatgtct caaatggaat atcatgaccg
    14761 tctttgttta agcgtgcacc acattttgga cattgttttt ctggcatgtc aaatccagaa
    14821 ccatacgtac catcttcaaa aaactcggaa tattggcact ctggacaata ataatgaggg
    14881 gctaacggat taacttcagt gattcctgtc atcgtcgcaa caaagctaga gccaacagag
    14941 ccacgtgaac ctactagata gccatcttca ttacttttat gcactagttt ttgggcgatc
    15001 agataaataa ccgagaagcc attaccatta atagaattca actctttttt taatcgtttt
    15061 tgaatgatct caggtaacgg atctccgtac atttgttttg cttttgtgta actcaattct
    15121 gatatttcat cttctgaacc tggaatcttc ggtgtataaa gttcatcttt gactggaatc
    15181 acttcttcgc agatatctgc gattttattc gtattttcta ctacgatctc tttcgctgtt
    15241 tcttccccta aaaagtgaaa tgcagttagc atctcatcgg ttgttcggaa atggacatcc
    15301 ggcaaactat gacgattcaa cggattcgct ccgcccatag aattgatcaa aatttttcgg
    15361 taaatggcat cttcttcgtt cagataatgt acattacctg ttgcaacaac gattttatcc
    15421 aaactttttc cgatttctac caaattttgg ataatttctt ccaaatcatg ttcatttttt
    15481 accaattctt gctcgatcaa aggcgcatag accgcttttg gcataacttc aatataatcg
    15541 tagaattttg ctcggtttct tgcctcttct accccttttt gcatcatggc ttcgaatatt
    15601 tctcccttat cacaagctga gccgatcaat agattttcac gcatcttttt caattgggag
    15661 cgtggtattc gtgggacgcg ttcgaagtat tcgacattcg acattgaaat cagtttgaac
    15721 aaatctttca gtcctgcctg attttttgct aaaatcgtta catggaaagg tcgagccctt
    15781 ttgtaagaat ctccttctcc gatatgctca tttagctgat catgataaag catgttatga
    15841 ttgtccattg cctctttcac aaagatccat gctaagtgac cagttgcttc agcatcatag
    15901 attgctcggt gatgctgttc caagctgaca ccaaattttt tagataacac acctaatccg
    15961 aaacgtttga attgaggata cagatatcta gctaattcca acgtatcaat gactggattg
    16021 gccgcttcag gaataccata ccttgcgtag ctagtattca agaaccccat atcaaaagcc
    16081 gcgttgtggg ctaccaaaat tgtatctttt gaaaattcaa ggaacatccg caatacttct
    16141 tcttccgact ttgatcctcg aaccattcca tccgtgatac cagtcagatc taccgttgtt
    16201 cgtgacaaag gatgtcctgg atcgataaat tcatcaaagc tttcgatcac atttccttta
    16261 tacattttga ctgcggctag ctcgatgatc gtgtcatata ctgcagacaa tcctgtcgtt
    16321 tccacgtcaa atacgacata tgtggcttca ctcaatgctt catgtgcatc attataagca
    16381 attgggacac catcatctac gacattcgct tccacgccgt ataagatttt cacacctgct
    16441 ttttttccag cactatgtgc ttccggaaag gcttgcgcac caccgtggtc tgtaatggcg
    16501 atagctcggt gtccccattt ccctgcttgt gccacaagat cagagatgct gtttgttgca
    16561 tccatcgtac tcatattgct atgaacatga agctctactc gtttttcccc ttcaggagca
    16621 taatctttgc gaggtgtatg tttgacttct atgatatctt gcgcattcat gaccaaatca
    16681 cggacaaacg tatcttcttg aatacttcct cgtaccttta accagctacc gacactgatc
    16741 gcatcaaata cttgttcatc tttttcgccg ttcgagaatt ttttgacgat aaatgatgaa
    16801 gtataatctg tgattttcaa agtcaagatc ttgcgttttg aacgtagttc tctgacttct
    16861 ttgtcgaata cgtacccctc aagtgtgact cttcgttctt cttcaacgat actgatcatt
    16921 ggtgtcgttg gttcatcggc tggaatattt cgtcctagct ggatgtggat gggtccttca
    16981 agagctggcg attgctcttt tctttccttt ttcttttgtt cgtggacaac tagactttca
    17041 gctgcttgct tcataaaggc ttctgcctgt tcttgtttgc gttcttcaaa aagtttcaat
    17101 actcgttccg cttgctgctc atccacttct ggttcgatac gaaattttgg gaatccgtaa
    17161 ctgacgaaca actcttctac taatggcaag tattgttgct tcaaataaga aatagctcct
    17221 gtactgtcga ttggcaagat gactttacga tcttttatca caggtacctg cgttttcaat
    17281 acttgctgtg ctagtggtgt atcgcattgc tgattttcca aagcttgggc ccagtaatct
    17341 tgcaagagct gttcatcgaa tgtctggtca tctgctaaga tattcagttt gacttgtgca
    17401 atatctttaa aagcgatagt cagatgctga gtcaatgacc gatatagcat aataggcaaa
    17461 attttggaaa aatgcagcgt aaattcccat aaacgcgatt tacgatggac cactacacta
    17521 tccatttcac cagcagtaag cagtggatga tttttttctg cttcctctag ttggatttgt
    17581 tccaacagtt tttcaaataa atctcgtttt tctgacaaag atatacgccc tttctgatga
    17641 gaaacaatgc aattgatcgc atcttatcct cttctttttc agtacctttt agtatactcg
    17701 ttttttctga gttttacatt aaaaaattgg aactgcgatt caaatcgcag ttccaatttt
    17761 aaaagcgaat catcagattg tcaaaaacag ctgattcagc tatttttatt ttctgtgttt
    17821 taacgcttat tattcgcctt ggttcaagag gattggtaaa gtattcacca attcttcttt
    17881 acgtacttca accatttcgc cagttttctt gattttcact tctacgattc catccactgc
    17941 ttttttaccg acagtgattc tgattggaca gccaatcaaa tcagaatccg cgaatttcac
    18001 tcctgcgcgt tcatttctgt catcaaccag tacttcatag ccagcttttg tcatcgcttc
    18061 ttctagttca ttcgtcaggt ttgtttggta ttcgtctttt agattcattt ggacgatatg
    18121 caaatcaaat ggcgcaattc cttttggcca gttgattcct tgttcatctg aatgttgttc
    18181 tacgattgct gataataaac gacttacacc gataccataa cagcccataa tcacaaattt
    18241 ttctcgtcca ttttcgtcta gtacagttgc tcccatagat tcactgtaac gagtgccgat
    18301 tttgaagata tgtccgattt cgattcctcg agtaaaggca agaacaccat tgttgtctgg
    18361 tgaaggatct ccttcttgta caaaacgaag atcttcataa gaaagtacag tgaaatcgcg
    18421 ttctggattc acatttttat agtggaatcc tgtttcgttc gctccagtaa tggcattcgc
    18481 taaatcttgg acataacggt ctgcatatac ttttacgtca tctgaaacac cgactggtcc
    18541 gacagaacca aactcagcac ctagatattt ttgtgcttct tcttctgttg cttcttctaa
    18601 aaagtcagca gataaaaagt ttttcagttt tacgtcattg acttcatggt ctccgcgtac
    18661 caaaacaagg acaggttgtt catccgcaat gaacaataca gacttgatga ttttttgagg
    18721 ttcaacagaa aagaaatctg ctacttcgtc aattgatttt acgttaggtg tagctacttt
    18781 ctccaattca agctgtgttt catgcgattt ttttgataca tagtagcttg tagccatctc
    18841 caagtttgca gcataatcac tttcagtcga ataacagatc gtatcctccc cgatttctga
    18901 gatcgccatg aattcttttg agtctttccc gcccattgcg ccaccgtctc cgataatcga
    18961 gcgaaattct aagccacaac gcttgaatac ttcggtgtag gctttttcgt aatctttata
    19021 tgtctcgtcc aaactttctt ccgttgcatg gaaagaatac gcatctttca tgataaactc
    19081 tcttccgcga agcaaaccga agcgaggtcg tttttcgtca cgatatttag gttggatttg
    19141 ataaagattt aaaggtaatt tcttatatga attgatttca ttacgcatca aatcagtaaa
    19201 agtttcttcg tgagttggtc caaggatcat ttttcgatca ttgcggtctt tgaaacgata
    19261 caagtttggt ccatatgttt cgtaacgccc ggattcttcc caaagttctg ctggcaagat
    19321 ggcaggcatc agcatttcta ccgctccgat tttttcaaat tcttcccgca taatgcgttt
    19381 cagtttttcc aatacacgat ttgctaatgg cagatatgaa taaatacccg ctgatacttg
    19441 gcggatatat ccagctctta gcaagatttg atggcttaat acttctgcat catttggaac
    19501 ttcgcgtaat gttggaatca acatttttga ttgtctcatt ctatatactc ctttatttcc
    19561 tcaaaatagt tatcgtttgt tattataccc tatttgtcaa ctttttctag aaaaagaatc
    19621 gttgaatatc gttccatgta actagcacca tcaacaacat taaaaatcca aagccagcta
    19681 atgtcaatat tccttctttt tcttgactta atggtttccc gcgaatccct tcaaaaatat
    19741 tcaagactaa tttcccgcca tctaaggcag gaatcggaag cagattgacg attccgagat
    19801 tcattgaaag aagcgccatc aaactaataa cagttgttac accttgattt gctgcttctg
    19861 aagacaattg gtacatcatg acaggtccgc ctaatttatc taaactgaaa cctgtaaata
    19921 gtgatcctaa ggctttgaaa atttccaatg aaccgctgaa agcttgacgc gtccctccaa
    19981 tgatcttatc cataaaacct gtattcattg gggcttttat accaagttgg ccaacttttt
    20041 cgccatttga ctctacagat ttcggtgtca cgtcgacaga ttgcacctga ccttctcgtt
    20101 cgatcttaaa gtctaatgtt ttatccggat ttttcgtaat gactgttgtc agatcattcc
    20161 atgaatggat ttcttttccg tccacactga ctacctcatc attttctttt aagcctgctt
    20221 ctgccgcagc tccattaggc atgatctctc ctacgcgatt agtgttcgtc acttgaacac
    20281 caccttgcat aaatgccaga atgatgaata acaggattgc caaaataaag ttattcatgg
    20341 gtccagcaaa gttggtcagc atacgctgcc acaatttagc cgattggaac tgtacgtctt
    20401 ttggcgcaat tctgatttct gttccgtcgt tttcaataat cgtcgcatcg tgatcaacag
    20461 ggtaggttac tacttctgtt tcgtctccat tcacataacc agtgatagtc agctcatctt
    20521 ctaaatcata acggctcaat tccatcggga tagcatttgt caattggatt ttcttactta
    20581 aatttatctt ctctacgatg ccgtcagaat ttagtaaaag agaaagaggc attcctggtg
    20641 ccatttctgt ttcatcatcc ccattgcctg ccattcgaac ataaccgcca ataggtaata
    20701 ggcgaagcgt atatgttgtc ccgtcttttg cttgatgccc ataaattttc ggacccatac
    20761 cgatagcaaa ttcgcgaacg aggattcctg atcgtttcgc aaagaagaaa tgaccaaact
    20821 catgaacaat cactaatata ccaaaaacga tgataaatgt cagaatcgtt ttcatagctt
    20881 ttttctcctt tacttgtttt ttgtgttttt tataaaacaa cgataaaaca ctataatttt
    20941 accatagtat accaaagaca ccaatagtat caatataatg aaaaataaaa acctgcgtct
    21001 tttatgaaat tcacaaagaa acgcaggtat acttactaaa taatgccaaa taaatgcatg
    21061 ataggaaata caaacagcaa actgtcgaaa cggtctaaga ttccaccatg gcctggtaag
    21121 atatttcccg agtctttcac atcataatga cgtttgattg cagattcgac caaatcacca
    21181 aattgtccga ctgctgaaaa aataatcgtc atcagaagca tgacaaacat accatgatta
    21241 aatagttctt ttgctgggta tagcaataaa taaagaaatg cgactacaac agcactacca
    21301 ataccaccga gcgcaccttc aatcgttttg ttgggcgaaa tatcaggact taacttatgc
    21361 tgaccataac gtcgaccaac catatatgct ccgatatcag ttgcccaaac aataaataga
    21421 ccaaatatca aaacagccaa gctttctgtt cgtgcattga caaaattctg gaaacctatt
    21481 cccacataca agctgacaag cacaggaaaa cctgcttcat caatcgtata cgtattttta
    21541 gaaataactg atgttcctaa aagaatcata actataagat aaaataacat aaatgtactt
    21601 acattctctg gaagaaagaa aaaccatctt tcttttggca aaacaagtaa cactgcacca
    21661 acggcggata aaactccttc aaaacttaag agtgtcaatc ctttcatccg aaacagctcg
    21721 taaactccta ttactgctaa gattgctgct gtaagctcaa ccgctatgcc tccgatccat
    21781 ataatcggaa taaacaagac tagcgccacg gctgctgtga ccacacgttg tttcatgact
    21841 gatccccttt ctcatcttct gtttttttta caccgccgaa tcgacggtct cgattttgat
    21901 aagaggcgat tgcttcttca agatgcgcac catcaaaatc aggccaaagc gcttttgtaa
    21961 aatacagctc actatatgcg atctgccata acagaaaatt actaattcgc tcttctccac
    22021 ttgtacggat catcagctca ggatcttgta actctttagg caagaaacct gtcattaaat
    22081 gcttagcaat caattcatca tcgatttctt ctgcgtgtat ttctcctttt gctacttctt
    22141 cggcaatttc ttggacagca gtcacgatct ctgctctgct tccataattc aaagcaaaat
    22201 tcaataccat tccagtattg ttttttgttt gctcaattgc acgtcttaca gcatcttgcg
    22261 tatgttctgg caatacgttt tcataaccca tgacatgaac tttgacattt tctttaatca
    22321 attctggtac gaacgtatca aagaagtcga caggtagttg catgagaaaa cttacttcat
    22381 cttttggtcg cttccagttt tcagtcgaaa aggcataaag cgtcaacact ttcactccaa
    22441 gccttgatgc tttttttgtg actttcttta ctgtttccat gccttcttta tggccagcaa
    22501 ctcgtggcaa acgacggttt tgcgcccaac gaccattgcc gtccatgatg atggcaatgt
    22561 gctgaggaat tggtccatcg gtattaaatt gaaattcgct tttttcttga atatatttgt
    22621 ttttttgcgg aaaaaaacgt aacattcttt ttcctcctat acttaaaaga ttcccactat
    22681 cttaagtatt atagcaatat ctaactgttt ttcctatgaa atcattgttt ttttatttaa
    22741 aaactgaata attttttctt tttttaattt ttaaataaaa gaaatataaa taaaaacaga
    22801 ggaagcggaa tgatccgccc ctctgttttt atgattttat taaacttcca ataattcttt
    22861 ttctttatca gcagcgatct tatctacttc tttgatgctg ttatctgtca atgtttgtac
    22921 gtctttttct aatccgcgca aatcatcttc ggtaatatca ccattttttt gttgtttttt
    22981 atattcatcg atcgcatcac gacggatgtt acgaatcgcg atttttgcat tttctgcttc
    23041 ttttttaaca tctttagcta attcttttct gcgttcttct gtcagttgcg ggataactaa
    23101 gcgaatgaca tttccatcat tggttggact gattccgata tcacttgcca taatagcttt
    23161 ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc attaaaacac gtgcttctgg
    23221 aatttggatt gatgccattt ggttcaatgg tgttggcgca ccgtagtagt tcacagtaat
    23281 acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt tgcccaagtt cacgttgaag
    23341 attttgtgct gcttttttca ttttttcttt tgcttctgtc ataattgcat ctgccatttt
    23401 tatttccccc ttacagttgt tccgatattt tcgcctaaga ttgcacggcg aatatttccg
    23461 tgttcattta aattgaagac aagcagtgga atatcattat ccatgcttag tgaacttgca
    23521 gttgaatcca tgacttgtaa tcctttggca ataacttcca gatgagtcag ttcttcaaat
    23581 tttactgcat ttgcatctac tttcggatca gcagaataaa caccgtctac attgttttta
    23641 gccatcagga taacatctgc gccgatttct gccgcgcgta atgctgctgt tgtgtctgtc
    23701 gagaagtacg ggttgccggt accgcctgca aagataacga tccgtccttt ttctaaatga
    23761 cgttctgctt tacggcgaat atatggctct gcgatttgac gcatttcaat cgaagtttgc
    23821 acgcgtgtcg gtacacccac gttttccaac gtatcctgta aagctaaggc attcataact
    23881 gtagctaaca tacccatgta atcagcttga gcacgttcca tgcccatttg tgctccgatt
    23941 tgtccgcgcc agatatttcc gccgcccaca acgattgcca tttcaacgcc cagctcgtga
    24001 acttctttga tttcttggat gatttctttg attactggtg gtttgattcc aaatcctgcg
    24061 tccccagcta aagcttctcc actcaatttt aagactacac gctgatattt cggtgtgacc
    24121 atgttcattc ctccaatatt ttctattgcc attttaccat acttagatga tttcgacact
    24181 ctaagactgg tttttcttgt tttgcttaaa aaaagggagc gcacaaaccg tgcgttcccg
    24241 tctgttattc aacagaatta ttttttcact tggctcataa cttcttctac aaagttatct
    24301 tcgcgttttt cgataccttc gccaacttcg aaacgtacga atgttttaac agttgcgcct
    24361 tttgatgcaa cgtatttttc aacagtcata tcaggatctt taacgaatgg ttggtccacc
    24421 aaagcgattt ctgctttgaa tttcttcaag cgtccttcta ccattttttc aacgatattc
    24481 gctggtttgc cttcgttcaa tgcttgttct gtaagaacag ttttttcatg ttctaattca
    24541 gcttctggaa tttgtgattc gtttacataa cgaggattga tagctgctac gtgcatagct
    24601 acgtctcttg caactgtttc gtcagttgtt ccatctagaa cagtcaatac ggcaatacgt
    24661 ccgcccatat gcaagtatcc accaaatgca gcattgtcgt ctttttctac aacttcaaag
    24721 cgacggaagc tgattttttc tccgataact tgagttgctt cgatcaagtc agattcaatt
    24781 gtacctttat ctgttttgat tttcattgca gcttccatgt ctgctggttt gttttcagca
    24841 acaagttctg caatttcttt tactaaatct tggaacattt cgtttttaga aacaaagtct
    24901 gtttctgagt ttacttcaac gattgctgca gtgttacctt tcacagcaac tgaagccaat
    24961 ccttcagctg caatgcggtc atttttcttc gcagcctttg ccattccttt ttcacgtaaa
    25021 agatcaactg ctttttccat gtcgccttct acttcaacta atgctttttt cgcatccatc
    25081 attcctacgc cagtcatttc gcgtagttct tttaccattt tagctgtaac gtctgccatg
    25141 tttaactcct cctgtgtgtt tttcttttta aaaaaagctg cttcaaagga gagtgaggct
    25201 tgtcgcccag cctttgaaac agctccatca ttcaaaatta ttctgctgaa gaattgtcgc
    25261 cttcaacaac atctacgatt tcttcgattg atgttgctgt gttttcttct gtgaaatctt
    25321 cttcaactac ttgatcttca ccttggtttc cttcgatgaa agcatcagcc atttttgaag
    25381 taatcaattt aacggcacga attgcatcgt cgtttgaagg gattactacg tcgatttcat
    25441 ctggatcgca gtttgtatca accatagcta cgattgggat atttaatttg tgtgcttctt
    25501 gaacagcaat acgttcttta cgtgggtcaa cgatgtacat tacatctggg attcttggca
    25561 tatcagcgat accgcctaag aatttttcaa gacgttcacg ttctttgttc aaaccagcaa
    25621 cttctttttt aggtagaact tcaaaagttc catcttcttc cattgcattg atttgtttca
    25681 aacggctgat acgtttttgg atagtatccc agtttgtcaa tgttccgcct aaccaacgat
    25741 ggttaacgaa gtattgtcct gcacgagttg cttcttcttt gattgcttct tgtgcttgtt
    25801 tttttgtacc tacgaataat gctacgccgc cttcttcagc aacgctcttc atgtaatcat
    25861 aagctgcatc tactaattta actgtttttt gcaagtcaat gatgtagatt ccgtttcttt
    25921 ctgtgaagat atatttcttc atttttggat tccagcgacg agtttggtga ccaaagtgta
    25981 cgccggcttc tagtaattgt ttcatagaaa ttactgccat gtttgtttcc tccgatttgg
    26041 ttttattttt cctcttctta tcttctacct tgccggcaaa ctttttcaag cacctaccga
    26101 caatccaata agaatgtgga tttgctgatg cataaagcac cctcagctat tatacagaca
    26161 aaagatccgt atcgcaagag caaatatact tttctgctca acattcaata gtttacctga
    26221 aagataaaaa gaattcaaga aaaacagtag atttctcttt gtttctttat tatccttttt
    26281 ccataaaata attttaggta taaaagacga atattattgg gcaatccttc ttttttttgt
    26341 tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat gcgcaaagct caaagacatt
    26401 tgctgattaa acaagtcatt gaagaattcc cgatccgtac gcaagaagaa ctattaaaca
    26461 aacttgggca ttatggcgtt accgccacgc aggcaacgat ttctcgagat atccgtgatc
    26521 taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg cttcgtattg tttcgcgaaa
    26581 aaggaactcc tgagactagg actgaggaag aggatcggct ggttcaaatg attgaagaca
    26641 ttgtattgaa agtcgaacga gtccactttt tgacgatcgt caataccttg ccagacaatg
    26701 ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc ctctattgtc agtacaattg
    26761 ctggattcga tacgatcatg gtcatctcgg aaaatgagga ggacgcaaaa cgagtagaag
    26821 aatttcttca ccatccaact gaagtatcac ttttttaatc agagcacgta ttcctaatat
    26881 ggaatgcgtg tttttttatt ttcaatattt tccttattga ataaaccttt acactcttta
    26941 caaaaataca tcatttttta ttcaaatttt tcaacacctt gattgaatca caataatcat
    27001 catttatatt ggatgttaac aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa
    27061 tgaaaggaaa tcatatgtat aaattattaa cttctggacc attgacgact tctgaaacag
    27121 tgaaaaatgc tatgctgatc gatcattgta cttgggatca agaatacaaa gaaattacac
    27181 aatggataag aaaaaaatta ttggatcttg ctcaagtatc ggaaggaata tacacaacgg
    27241 tcttaatgca aggtagcagc tccttcgggg tagaatcggt attaagttct gtgatcaggc
    27301 cagaagatac tgtactgatt tgttcaaacg gcgcatatag acaacgaatg atcaacatgt
    27361 gcgaatgtct gaaactgaac tataccgttt atgaggtatc ggaacatcaa atcctccaag
    27421 ctcatgtgat tacacagctg attgaagaga atccgtctat ctccgcacta gcaatggtcc
    27481 atagcgagac tacttccggt attttgaata atttggaaga gattgctgct gtcacacaag
    27541 aaagagatct tttgtttata gtggacgcga tgtcaagctt tggtgggata ccgatttgtg
    27601 tagccgaact agggattgat tttttgattt ccagctcaaa caaatgtatt caaggtgttc
    27661 caggcttttc ttttgtactt gctaataaag cggaactgga aaaacgaaaa aatgttagcc
    27721 gaagtttgtc acttgatcta tatgaccagt accgcgtgat ggaaaacgag aatggcaaat
    27781 ggcgtttcac ttcccctacc catacggttt tggcttttca acaagcgctg aaggaactag
    27841 aggcagaagg aggaatcgaa actcgatatt cacgttatct cgccaataat catgtgctac
    27901 gacaaaaaat gacagagatg gggttccagc ctttcattca agaatatcag gggccattca
    27961 tcacctcttt tctttaccct aaggactggc gatttgattt taatcatttt tatcagtatt
    28021 taaagcaaaa tggatttgtg atctatcccg ggaaaatcag cgatcatgaa tgttttcgta
    28081 tcggaaatat tggcgagatc tatccggaag atattttgga actgacagca attatcaaag
    28141 actataagga gaacgttaat gattaaaact gtaatttttg attgggcagg aacaactgtg
    28201 gactttggct gtatggctcc tgtccacgct tttagaaatg cttttctaga aaaaggaatc
    28261 caactgactg acaaagaaat ccgctaaccg atgggaaaat taaaatggga tcatatccaa
    28321 cagcttcttt ccctcccttc tgtcaaaaaa caatggatag ctatttatgg acaactacct
    28381 caggaaaaag aggtggagga attgtatcaa caatttgaac attatctgtt taaagatttg
    28441 gtcaagcatt ctaccttgaa acccgacaca ttggaaacga tcgaacggtt gagaaacgct
    28501 gggatcaata ttggttccac gaatggattt aatgctccta tgatgacaat cgtagcagaa
    28561 accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg
    28621 tatggtcgac cgtttcctta tatgatccat aaaaacatgc agtacttcaa aaacaagtcg
    28681 gttgatgaag taatcaaagt cggagatacg gtctcagata tccaggaagg gaaaaatgcc
    28741 ggagtgttga cagtaggtgt aattgaaggt agttcgctaa tggggatttc ttatgatgag
    28801 taccaaaaat tagagatggg gaaaaagatc gtttaaaaca gtctgttgct cttagattcc
    28861 aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga gttaactgaa tacattttgt
    28921 acgctgacat gaatccagaa tgacttttat acaaaataag aggttgtgaa aaagctgtcc
    28981 tgtatcaagt aataaaaaca gccaagcaaa aatagctcct catatttttc gtttggttag
    29041 gcttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa aagggagctg
    29101 tgacacgact tttgtcacag tccccttatt tgcaaacaaa cagtgtttaa gagacaacct
    29161 ctccactaaa cggtgttcat tcagctgact ctcggtattt gctcaaaaaa cgataaaatt
    29221 tgaaaagcta ttttctcttt tgtttctcta atactcggat cagaacgctg atttcacatt
    29281 ctctttttct tacatcttat ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa
    29341 taatacagtc actgcataaa ctagagctaa gcgtgcttct ttttgttcat catcagctaa
    29401 aatcttcgta tgagcatagt atttgttgaa tgcttgtgct aatttgattg catgtttcgc
    29461 aatcactgac ggttcgtatt tttccccagc agacaagacc gtttcagggt atttttgaac
    29521 taatttaacc acttcccagc tggtatcgtc attcaatgca tagtctgctg tttctgaagg
    29581 gacaaatcct gctttttcca acaggctgac tgcacgagca tgtgtatatt gcacataagg
    29641 tccagtttct ccttcaaatc gaacaacttc ttccaggtta aagtcaaatg tattcagtcg
    29701 atcgtttttc aaatcgtgga aaatgactgc cccaacccca acttgtttag ctacagcatc
    29761 tttgttttcc aaatcagggt ttttctcgct gatttgttct ttagcagatt ggattgcttc
    29821 atttaatact tcttctaata agacgatctt tcctttacga gttgataatt ttttaccacc
    29881 ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca gaccagtcaa agcccatttc
    29941 cttcaagaca gctttcaact gtttaaaatg atagctttgt tcattcccta ctacatatag
    30001 agattgtttg aagtcgtaag tacgtttacg gtacaatgca gcagctaagt cacgtgtaat
    30061 gtatagtgtc gcaccgtctg acttcttaat caacgctgga ttcaggtcat aagcagatag
    30121 gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac
    30181 gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc
    30241 cagcagatca tagattttat tgaattcttt catcgattca tcacggaacc attgccataa
    30301 ttgaatagcc tctttatcgc cttcttccag acgtttgaac caagcacgag cttcttcatt
    30361 taattctggt tctgtttcag ctacttcatg gaattggacg tataaacgaa gcaattcatt
    30421 gattggttct gctttaacag cttcttctgt accccatttt ttgtaagcga cgatcagttt
    30481 tccaaactgt gttccccagt cacctaaatg gttgatgcgg attggctgat aaccgatttt
    30541 ttccataatg aaaccgatcg aattaccgat aactgtcgaa cgcaaatgtc ccattgagat
    30601 tggtttggca atattaggag aagacatgtc gattggtact gttccttgat tcccgatgtt
    30661 gctatcccca taatgttctt tttctttcac tactgtttgc aaaacttttt tgctgataag
    30721 ttctttattc atgaaaaagt tcaagtaagg tccgacaact tcgatttttt caaagttggc
    30781 agaatcgatt ttttctgcta aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt
    30841 tgctaatgaa aaggctggaa atgcaacatc tccgtgttca gctgattttg gattttctag
    30901 taattgctcg acttgttcca gtgttaaatc atctttgaca gcgtcataga cagcttttgc
    30961 gacaagatct ttattattca tctttattcc tcctaaagtg ttcccttgat tgttaaacaa
    31021 aaaaaatctc tagcgacggc atacttagta tgccatcact agagacgaga tttcccgcgg
    31081 taccactcca attgtcctaa aaaaggacca gctccatctg atatcggtag acgaccgtct
    31141 gtttaaaaca gaattctcca agtgcgcttc gttgtatctt cttcattcgg cttccaccac
    31201 ttccgaattc gctagccaag aatgatacaa gtactctctt gttcaacaaa tcttagtctt
    31261 attaaacttt cttgaattat atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa
    31321 acaatccaga tggcctaaag gagaagaaat accacctgga tagctataaa tcatttcgta
    31381 aatacgaaag acttaacaaa ttaatcagct acgataatcg ttccttcttc agaagctaaa
    31441 agattctcga tattttctaa agaagtgatg attgccttag cgtttggttt tgcttcaaca
    31501 aatgcaatag ctgcttctac tttcggaagc atgcttcctg gagcgaattg cttttcatca
    31561 atgtattgtt tcatttctga aacagtaaca gtttctaatt tcttttgatc tggtttttgg
    31621 taattgacat acacattgtc tactcctgtc aatacgatca gcaaatccgc atcgatgatt
    31681 tcggctaatt tttctgaagc aaaatcttta tcgattacgg cttctcgtcc ttctaatcca
    31741 gtagctgttt ctacaacagg aatcccaccg ccaccaacag agactgtgat aactccttga
    31801 tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga tgggtttagg ggaagcgact
    31861 acttttctcc atccacgtcc agcatcttct ttgaatgtca cagtggagtc agcattcatt
    31921 tgttctttgg cttcttcttc cgtgtagaat ggaccgactg gtttacttgg atttttgaat
    31981 gaaggatcat tttcatccac aataacttga gtgactaaag aaaccacatc tttatcgatc
    32041 cctttttcct tcagtacttc gcccatggcg ttttgtaacc agtaaccgat agatccttct
    32101 gtcattgcaa cacatgtatc taaaggcatt gccggtgttt tctcagaatc agcagcttgc
    32161 tgctgaatca ataggttacc gacttgcgga ccatttccgt gggagatgat caattcatct
    32221 ccttgttcga taaatttcac taaatacttt gctgtttcca ttaacgcttc ttgttgggct
    32281 tttgcacttg catccgtaga caagatggca ttgcccccta aagcgacaac tacttttcgt
    32341 ttggccattt cattcgtcct ttcttcagtt ctaccgaatt ttctagataa caaaacgagc
    32401 cagcgttttg tgattttccc tcaatgctga ctcgtttttt cgtgagatga atgtggttat
    32461 gcttaaacgc gagggataaa caagttacct aatgttgctg ccatgatcgc tttgattgag
    32521 tgcatacgat tttctgcttg gtcaaattga cgagcatatt tgctgcggaa gacttcatct
    32581 gttacttcca tttcagtgat gccaaagcgt tctttcattt gttcaccgta aacagtgttt
    32641 gtatcatgga aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt
    32701 tctaccattt ccatattgat ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta
    32761 tcttcttcac ccattgatac ccatacatca gaataaagga cgtctgctcc gtctacacct
    32821 ttatcaacat cgtcggtaat catcaattgt gcaccggatt tttcagcaaa gccttctgcc
    32881 attttgacga tttcttcttc aggttgtaat tcttttggtg caacgatacg catattcgct
    32941 cctaaaattg cacctgtgac taataaagag ttagccatat tgtttcgacc atctccgcag
    33001 taagcaacag tgataccttc tactgttcca aagttttctt ggatagttaa aaagtcagcg
    33061 atcatttgtg ttggatgcca ttcatcagtc aagccattcc aaactggaac accagagaat
    33121 tctgctagtt cctctaccat tttttgactg aatccacgga attcgattcc gtcaaacata
    33181 cgtcctagta ctttagctgt atcttctgtg gattcttttt tacctaattg gatatcattt
    33241 gcacctagat attcaggatg tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga
    33301 gtacgtgtag atgttttttc aaataaaagc gcaatatttt ttccttctag atagtgatgt
    33361 gggataccac gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa atattgtaat
    33421 tcttcttttg taaagtcttt ttctgctaat aagcttcttc cttggaatac tgattctttc
    33481 atattaaaca ttctccttta gattgttttt atttaagatt aaagaggaaa tcccgacggc
    33541 agctgacagt aagactgccg tcaggaaaca aattacttca agtcttcacg gacaagtggt
    33601 tggctcatgc agcgtggacc accacgacct cgtgacaatt cacttgaatt gatttctaac
    33661 actttgatgc catagctacg aagcaattcg ttcgatacat aatttcggtt ataagtgact
    33721 acaacacctg gagcgattgc caatgtgttt gatccatcat tccattgttc tcgaggtgca
    33781 acgatctcat ctccattacc agttgggatc agtacaagat catctaatcc aagagcagct
    33841 ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga tatcatcacc atctggttcg
    33901 attgtaaaga catccatctt accgtcttta ctttgaattg ctggatggat agtgaattta
    33961 tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt gttcggaatt
    34021 ttgattgcca acactttttc aaatcctgaa ttttttgcga ataggtggcg tgctaatttt
    34081 tctaaagctt tcgcattagt tcgttgagaa atcccaacag ctactacttt atcgcttaag
    34141 atcaattcgt ctccgccttc gatatgatct ggattttcac gatccaacca tacttcgatt
    34201 cctttatttg caaatcttgg atgatgtttc aaaatatatt ctgtaaacat cgattcacga
    34261 cgacgtgctt caaaagtcat cttattgact gtcatcccat ttcccattga tgcggaaggg
    34321 tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa atggatagtc atcgtctgct
    34381 gacagatcat atagactgct agaacgtact tcgatatctt ttgtacgaac cccagccatg
    34441 attttatcta ccatttcttg cgtttccatt gatagaagga actcatgaag accctctctt
    34501 acagcattcg atgcaatatg agattcatcc aacattttat tcaagaattg ttctttgaca
    34561 ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt aaagagtctc gacgccttca
    34621 ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag caatcggaag atatggaata
    34681 tcatcgaaca acagacgatc cataatatct ggtgtcaaat tttccacttc ttgtccaggc
    34741 cgtttcagta aaactgtctt taatttccct atttcagaga aaacgtgaat aggtttatcc
    34801 atgttgtgtt gcctccttct ttttgattac atgtatagga tatcctcctt ttgtaatcac
    34861 tttcataatt ttgtctcatt caaaatgaga cattcctcac attaatattc gataattaaa
    34921 taattaaacg tttttctatt tttaagcgta atttattcat tttttattct gtatacactt
    34981 tcaaaaaaat atttattcat tgaaaaaatt tttttgaata aattaaaaaa ataaattata
    35041 ttatcgttgt tagcgtttac tatatgtatt attgttgttt tttatacaca aatcattcgc
    35101 ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa ttatatttgt
    35161 aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt ctttaaaaaa
    35221 agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt taataaaatc
    35281 gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc ggatgaagag
    35341 ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca gatccttttc
    35401 gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt tcgtttagaa
    35461 cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa taaccttttt
    35521 cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc gatgactgat
    35581 attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa
    35641 cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga attgcgtatc
    35701 caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt cattttgaaa
    35761 gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc gatcacctta
    35821 aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa
    35881 ctacgtgatt ccgggctgtt agattatgac aaaaagcgtt ttactttctg taagacagct
    35941 actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct gataaatatc
    36001 agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt ttagatcact
    36061 ggacaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa
    36121 aaatactgtt agattaacta gatgaagcgt cttgtcctca tccgatctag attattcgat
    36181 attcctcgac aataaacgat tactcagtga gggcaatcag aggaacggat aatgataaat
    36241 aagctgtaaa aaatagcaaa ataacaataa tgattaaaag tatgtaaaaa atagggcttt
    36301 gacaaaaacg tgtcacagcc ctatttcgat taaacggtgt gtacctgtca actcttcggt
    36361 cacaagtcat aatgttcata aacatgagga gctgtttttt gaaattgttc cttgtgaatc
    36421 agagttaaac gacaggctta cgaactcttg attaaacggc gttcaaaagc ttgcatacgc
    36481 ggtaataaga acagccaggt caaaaacatg agaagccgtt tttcctgggc tgttcttatt
    36541 acttgatgca gggcagcttt ttcacaacct ctttattcag ataaaaccaa gcttggatca
    36601 gcattcaact gatagctagc aaaatttctt ttttgcattt ccacatgtgc tgctgctcca
    36661 atcattgcag cattatctcc acaaagtcga agagggggaa tcactagttc tacctcaggg
    36721 agtttagctg ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc cgctacacct
    36781 ccagctataa tcagttgctt gactggataa ttttgacaag ctcgaagtgt tttagtaatc
    36841 aatacatcga tcacacttgc ttgaaagcta gctgctaaat catttttgtc tagatcttct
    36901 ccacgctgtt gtgcattatg caccaaattg ataaatgcac tttttaatcc gctaaaactg
    36961 aaatcataat tatcttcatg gatcatcgct cgcggaaaat ggtaattatc ttttccctga
    37021 tgagctagct gatcgatttc ttttccgctt gggtaagaaa gacctaatac tcttccaact
    37081 ttgtcataag cttctcctgc tgcgtcatct cttgtctccc caatgatttc atatgatcca
    37141 tcttcttgca tatagactaa ttctgtatgt cctccgctaa caagaagtgc catcaatgga
    37201 aactggaatg gtttaaccag acgggctgca tagatatgcc ctgccatatg gtttacagga
    37261 ataagcggca attggtgtgc ccaagcaaaa gccttagctg cagaaatacc aatcagcaac
    37321 gagccgacaa gtcctggtcc ataagtgaca gcgactgccg ataaatcttc tgccgacaca
    37381 ccagcttcta ccagtgcatc ttccaagcat aaggtgattt gttctacgtg atggcggcta
    37441 gctacttcag gtactacgcc accaaaacgt ttatgactgt ttatttgaga agcaacgatg
    37501 ttagataaga tctctgttcc atttctaacg accgctacac tcgtttcatc acaacttgat
    37561 tcaatcgcca gaatcaattc ttcattcatt tcatcgtctc cgtttttaac tttctgctca
    37621 taatgatcgc atcttccgtc ggattgcgat aatatgattt ccgtttacct aatatacgaa
    37681 acttttctga ttcataaagt tttcttgctg cttcattaga tagacgtact tctaaaaaaa
    37741 catgcagcga atcttttatt tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc
    37801 cttttccttg ttctgaaact gcaacagcaa tatttgtgat ctcgacttca tctattactt
    37861 tcgagtatcc aataaagcca cagatctggt tatcttcagc taaaagcaga taatcagagt
    37921 gaggctgttg gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca tgtggataag
    37981 cattttcgct aatcatccat acgcattctg ccaagtacgc ttcatcaaaa tctttttttc
    38041 tgcaaatcaa ctaatctcac tcattttcta aaagttttat catatccaac gcatcttctt
    38101 ttgtttcagt gtaatatctt tttttgatcg ttgttgcttg aaaccccaat ttccgataaa
    38161 gtctttgtgc atcttgatta ctcatacgca cttctaatga catcgtctca cagcgattca
    38221 ttatagcaaa gttctcgatt tcgtcaataa gcagcgaacc gattcgttta cgctgatatg
    38281 ccggtcgaac ggcaatattc gtgatatggc aatctaaacc gaacgctctt gaaccgataa
    38341 aaccaatcat tcttccatta tcaaagatac ctagatatag atgaggaagc ggtgacttca
    38401 attctgatat aaaggcactt tttgtccatg gcaattcgcc attatagact tctcgttcga
    38461 catccagcag ctctttgata tcttctgtag tcaattctct tagtacgtaa cgacgatcga
    38521 tttgttcgac tgttttttct gtataaatca ttttatcttg aaaaaagctt ctggcatacg
    38581 ctctaaattt tttcaacata actttcgtct ccaggtgtgt gcgtctccaa ccatttttct
    38641 tctgcttcta ctcgttttaa atagcgagga agaaaagctt gtttctctgc aactggttca
    38701 actaaagcac ctaattgagc cacagttaca ccacttggaa tatcccatgc tggaactgta
    38761 ttaatcatcg cactaggcaa tatttccttt atttcagaga agaatttgtg acaatcactc
    38821 ccgacgaaat aaatagcttc attcagcttt tgtagttgtg tgaacaaatc agaagcagaa
    38881 atatgtacat cttcaagtac agtttctaat ctattttctt tccagcggta tgccccagcg
    38941 tatacatttt ttcttcgagc atcaaacatg ggcacaataa tattatccac accgatgcaa
    39001 ttagccgcga tggttttcag actagatact cctactagct ctttatctaa tgtataagca
    39061 atcgtttttg ctgtcgttac acctatacga agccctgtat aagaaccggg accatcagaa
    39121 accgcgattc ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa ttggtcgatt
    39181 gcaggcatca gtgttacact gtggttgcgt ttgatatttg tttgtatttg tccaagaatt
    39241 tgttcgtctt ctaccacacc gatcgccaaa gtttggtttg cagtatctat tcctaatgta
    39301 atcatatatt tgatcctttc tgctatgaca ttctcgcctt ccattgtagc atactttttt
    39361 attctgagga tgaaaatacc aaaggcaaaa ctagtacaat aatacatgat tatctgctgg
    39421 cgggcaaatt ttgccccttc ctgaaacaca aaaaagctgc ggtatcactc cattttccca
    39481 cagcttttcc atcttttatt tcgttgattc tcgaataatc aactctgttt ccacaataat
    39541 ctttcttgaa accggtgctt tggaattact taattgaaga atcgtctcca ctgctagctc
    39601 tcccatccat tcggtataaa cttttacagt agaaagcgag ggcgttacat atttcgctac
    39661 actgacatca ttgaagccca tcacagaaat atcttctggt accactaaac ctttttcttg
    39721 aatcgctttc agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg catcaggaac
    39781 atgcggattc gtttttaaat attgcttcac tgcttgatat cctgactcga cagaaaaatc
    39841 tgcttcgata attggacctt cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat
    39901 aattctggga tcttttagcc gctgatggtt tttcttcgta tattctgttc cagcaagcat
    39961 agcgatatgt tgcgctttat gggagacaaa gtgttcaatg actttttgaa cacttgactt
    40021 aaagtcgaca actagagagt cgattccttg agaagtgccg tcagaatcta ccaaaagcaa
    40081 ggcattttgg gatcctttca tttctaataa ctgagcttcg tcgaattttc ctagtgcgat
    40141 cgttccatct gccggaagat cactaatgga atcccacgtt tctttcaata aagtaacgtt
    40201 tgcttcttct gccttttttt cgattcctag gcgaatagat aaataataaa gatcttcaag
    40261 ctcttccgta tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat ttgctgcatt
    40321 tttacgatgt ttcgtataat ttaagcgttc tgctgcttca aaaatttttt gctttgtggc
    40381 taagccaacg gaaagatcag gatcatagtt gagaactctt gaaacagtag caggagaaac
    40441 tcctgccaac tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct tttttacgtt
    40501 tataaaatcg cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg cttgaatact
    40561 ccagcgtctt ccagtacacg tgcaaaaacc tgacctacag atttttgtac gacatcggtg
    40621 accgtatctt tcgtcaaatc aggatgttct ttttttaatt gttctgccca cggacgatga
    40681 tattcagcca tctggttttc ttctcctaat aagaaatgct ctacttctac tagttctggt
    40741 ttcaaacgag gtggtaaaac agcaagcccc attacttcga tcaacccaat gttctccttt
    40801 ttgatatgtt ggacatcttt atgtggatga aaaatcccat caggatgttc ttctgaaaca
    40861 ttgttgtctc gaaggacaag gtccaactca aagtaattgt cccttcttcg tgcaattggc
    40921 gtaatcgtat ggtgtggtgt accatctgct gaaaaagctc tgatagatac tgtttcatcc
    40981 gaatatgctt tccatttttc caatactgag ttagctgctt caataagcaa gtttttctct
    41041 gttgattgca aacggatcac agacatcggc catttcacga ctcctgcgat gacttgcgga
    41101 taatcttcta gtttgataag tttttctatc ggtgcttttg ccataggaaa ttcatgacgt
    41161 cctgcctgat aatgatcatg agaaagaatc gatcctccaa caattggtaa gtcagcattc
    41221 gaaccaacaa agtaatgagg caagacttca gtaatccgaa gcagtctagc aaatgtttcc
    41281 tttgtgattt tcatcgggcg gtgttgttct gataaaatga tcgaatgttc attgtaataa
    41341 gcataagggg aatattggaa tccccagctt tcaccgtcta gattcattcg aataatccgg
    41401 tgattggtgc gggctggata gttcaagcga cctctatatc cttcgttttc catgcaaagc
    41461 atgcaggtag gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct
    41521 ttttctggct tagataaatt gattgtgatt tccagttcac catactccga ctctgccgga
    41581 aatttgatgt tttttgcgat ggctcttgtt ttgatgtagt catttttctt acttaaatta
    41641 aagaaatact cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg
    41701 acgactgaag gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat ctcttgttct
    41761 gctggcaatt ctgaaagaac accattttct cttgccttag cgacaagctg atccaataag
    41821 tctacagatt cttccttaac tggtcttact tcaatgcttt ctagagcatc ttctcctacc
    41881 atccctaaaa gacgattctg taaatataag cggtccattt ccatccatcc gccagcttcg
    41941 atcgcaagtg tggcaaaatc acaaatcgtt tgactgattg acatatctca ttcccctatt
    42001 cttcataacc atttggatgg ctgacatgcc aatcccatgc tgttttgata atcgctttga
    42061 tatctgtgta ttcaggtttc cagcccagaa tctctctcgc tttttcactt gaagcgacta
    42121 atcggctagg atctccagct cttcttggtg ctatttctgc tggaatttct tttcctgtca
    42181 cttctctagc tgcttctagc atttccttga ctgagtatcc tttgctgctg cctaaattga
    42241 agaagttgct ttcatttcct tctttcaagt attccaaagc taaaatatgt gcagcaatca
    42301 aatcttctac ttgaacataa tctctgatac atgttccatc tggtgtatca taatcgtctc
    42361 cataaacagc caaggctttt ctttgaccaa gagctacttg taaaatgatt gggacaagat
    42421 gcgtttctgg tgtatgatct tctccgatag atgcatctgc ttttgctccg gctacattga
    42481 agtagcgcaa agcgacatag cgcatgccat atgcttggtc acaccacttc atcatttttt
    42541 ccatcatcaa tttactttca ccatacggat ttttaggatt agctggtgta tcttctgtga
    42601 ttggagactc tttcggctct ccataagttg ctgcagtaga agagaatacg attttattca
    42661 cgttgaactc gtgcatgact tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat
    42721 acatcaatgg tttttcgaca gattctccta ctaaagagct tgcagcaaaa tggattactc
    42781 cttcaatcgg ttccttttca aatacggaac gtagaaattc tttatcccgg atgtctcctt
    42841 cataaaaatg agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca
    42901 cagctacttg ataccccttt tgaactagct ggtctacagc gtgcgaaccg atataaccgg
    42961 ctccgcctag tactaaaatt gtcataaaaa acactccttt gattagtaaa attgtgtttg
    43021 ttgattctat ataatttaaa aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa
    43081 atttcgtaaa ttcatatggt aaatttataa taaaacattt agtaaacatt ttcaagggtt
    43141 ttttgcaaag gtttacaaac aaacgtaaaa ctagcctttt tttctctttt gttccataat
    43201 agatataaca aat
//
LOCUS       DACDBG010000018        42914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDBG010000018 DACDBG010000000
VERSION     DACDBG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.018"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IVZ90_000928"
     CDS             591..1280
                     /locus_tag="IVZ90_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8261995.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IVZ90_000929"
     CDS             1267..2475
                     /locus_tag="IVZ90_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8261996.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IVZ90_000930"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IVZ90_000930"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8261997.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IVZ90_000931"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IVZ90_000931"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8261998.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IVZ90_000932"
     CDS             complement(6578..7273)
                     /locus_tag="IVZ90_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8261999.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IVZ90_000933"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IVZ90_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8262000.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IVZ90_000934"
     CDS             complement(8472..8663)
                     /locus_tag="IVZ90_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8262001.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IVZ90_000935"
     CDS             complement(8684..9574)
                     /locus_tag="IVZ90_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8262002.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IVZ90_000936"
     CDS             complement(9561..10328)
                     /locus_tag="IVZ90_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8262003.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IVZ90_000937"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IVZ90_000937"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8262004.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IVZ90_000938"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IVZ90_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8262005.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IVZ90_000939"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IVZ90_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8262006.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IVZ90_000940"
     CDS             14944..15282
                     /locus_tag="IVZ90_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262007.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IVZ90_000941"
     CDS             complement(15450..16208)
                     /locus_tag="IVZ90_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8262008.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IVZ90_000942"
     CDS             complement(16226..17044)
                     /locus_tag="IVZ90_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8262009.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IVZ90_000943"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IVZ90_000943"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8262010.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IVZ90_000944"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IVZ90_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8262011.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IVZ90_000945"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IVZ90_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8262012.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IVZ90_000946"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IVZ90_000946"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8262013.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IVZ90_000947"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IVZ90_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8262014.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IVZ90_000948"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IVZ90_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8262015.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IVZ90_000949"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IVZ90_000949"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8262016.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IVZ90_000950"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IVZ90_000950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8262017.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IVZ90_000951"
     CDS             27086..27385
                     /locus_tag="IVZ90_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8262018.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IVZ90_000952"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IVZ90_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8262019.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IVZ90_000953"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IVZ90_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8262020.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IVZ90_000954"
     CDS             28352..30325
                     /locus_tag="IVZ90_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8262021.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IVZ90_000955"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IVZ90_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8262022.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IVZ90_000956"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IVZ90_000956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8262023.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IVZ90_000957"
     CDS             33204..34496
                     /locus_tag="IVZ90_000957"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8262024.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IVZ90_000958"
     CDS             35232..36095
                     /locus_tag="IVZ90_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8262025.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IVZ90_000959"
     CDS             complement(36224..37846)
                     /locus_tag="IVZ90_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8262026.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IVZ90_000960"
     CDS             38095..38616
                     /locus_tag="IVZ90_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262027.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IVZ90_000961"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IVZ90_000961"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8262028.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IVZ90_000962"
     CDS             40697..41515
                     /locus_tag="IVZ90_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8262029.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IVZ90_000963"
     CDS             complement(41618..41815)
                     /locus_tag="IVZ90_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262030.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IVZ90_000964"
     CDS             41984..42562
                     /locus_tag="IVZ90_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8262031.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13026 a         8235 c         8476 g        13177 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttagc ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaa
//
LOCUS       DACDBG010000019        38570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDBG010000019 DACDBG010000000
VERSION     DACDBG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.019"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..657
                     /locus_tag="IVZ90_000965"
     CDS             34..657
                     /locus_tag="IVZ90_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262032.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="IVZ90_000966"
     CDS             1107..2156
                     /locus_tag="IVZ90_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8262033.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="IVZ90_000967"
     CDS             2153..3034
                     /locus_tag="IVZ90_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262034.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="IVZ90_000968"
     CDS             3037..3807
                     /locus_tag="IVZ90_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8262035.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="IVZ90_000969"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="IVZ90_000969"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8262036.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="IVZ90_000970"
     CDS             5118..6281
                     /locus_tag="IVZ90_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8262037.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="IVZ90_000971"
     CDS             6398..6808
                     /locus_tag="IVZ90_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8262038.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="IVZ90_000972"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="IVZ90_000972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8262039.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9348..9665
                     /locus_tag="IVZ90_000973"
     CDS             9348..9665
                     /locus_tag="IVZ90_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262040.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9825..10061
                     /locus_tag="IVZ90_000974"
     CDS             9825..10061
                     /locus_tag="IVZ90_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8262041.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="IVZ90_000975"
     CDS             10058..10456
                     /locus_tag="IVZ90_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8262042.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11123..12067)
                     /locus_tag="IVZ90_000976"
     CDS             complement(11123..12067)
                     /locus_tag="IVZ90_000976"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8262043.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12363..13307)
                     /locus_tag="IVZ90_000977"
     CDS             complement(12363..13307)
                     /locus_tag="IVZ90_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8262044.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13528..14742
                     /locus_tag="IVZ90_000978"
     CDS             13528..14742
                     /locus_tag="IVZ90_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262045.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14764..16779
                     /locus_tag="IVZ90_000979"
     CDS             14764..16779
                     /locus_tag="IVZ90_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8262046.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16862..18235)
                     /locus_tag="IVZ90_000980"
     CDS             complement(16862..18235)
                     /locus_tag="IVZ90_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8262047.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18261..18545)
                     /locus_tag="IVZ90_000981"
     CDS             complement(18261..18545)
                     /locus_tag="IVZ90_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8262048.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18547..20562)
                     /locus_tag="IVZ90_000982"
     CDS             complement(18547..20562)
                     /locus_tag="IVZ90_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8262049.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20741..21046)
                     /locus_tag="IVZ90_000983"
     CDS             complement(20741..21046)
                     /locus_tag="IVZ90_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8262050.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21135..22166)
                     /locus_tag="IVZ90_000984"
     CDS             complement(21135..22166)
                     /locus_tag="IVZ90_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8262051.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22373..23248
                     /locus_tag="IVZ90_000985"
     CDS             22373..23248
                     /locus_tag="IVZ90_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8262052.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23400..24242)
                     /locus_tag="IVZ90_000986"
     CDS             complement(23400..24242)
                     /locus_tag="IVZ90_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8262053.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24334..26211)
                     /locus_tag="IVZ90_000987"
     CDS             complement(24334..26211)
                     /locus_tag="IVZ90_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8262054.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26208..27629)
                     /locus_tag="IVZ90_000988"
     CDS             complement(26208..27629)
                     /locus_tag="IVZ90_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8262055.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27632..31021)
                     /locus_tag="IVZ90_000989"
     CDS             complement(27632..31021)
                     /locus_tag="IVZ90_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8262056.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31278..32669
                     /locus_tag="IVZ90_000990"
     CDS             31278..32669
                     /locus_tag="IVZ90_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8262057.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32742..33389)
                     /locus_tag="IVZ90_000991"
     CDS             complement(32742..33389)
                     /locus_tag="IVZ90_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8262058.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33397..34083)
                     /locus_tag="IVZ90_000992"
     CDS             complement(33397..34083)
                     /locus_tag="IVZ90_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262059.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34089..35162)
                     /locus_tag="IVZ90_000993"
     CDS             complement(34089..35162)
                     /locus_tag="IVZ90_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8262060.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35384..36058
                     /locus_tag="IVZ90_000994"
     CDS             35384..36058
                     /locus_tag="IVZ90_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262061.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36063..37403
                     /locus_tag="IVZ90_000995"
     CDS             36063..37403
                     /locus_tag="IVZ90_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8262062.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37552..38538)
                     /locus_tag="IVZ90_000996"
     CDS             complement(37552..38538)
                     /locus_tag="IVZ90_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8262063.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11871 a         7042 c         7306 g        12351 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta cataatatat
    10681 attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat
    10741 cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct
    10801 tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat
    10861 atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc
    10921 acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc
    10981 atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct
    11041 attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt
    11101 tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg
    11161 ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa
    11221 caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac
    11281 cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga
    11341 tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat
    11401 aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca atacgatggt
    11461 ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa
    11521 cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta
    11581 atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa
    11641 cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt
    11701 ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc
    11761 gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga
    11821 taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata
    11881 cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg
    11941 ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg
    12001 cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt
    12061 ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc
    12121 ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga
    12181 aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga
    12241 taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata
    12301 ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct
    12361 tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg
    12421 aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc
    12481 gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag
    12541 gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga
    12601 gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta
    12661 agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga
    12721 gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat
    12781 gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg
    12841 ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa
    12901 gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct
    12961 tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta
    13021 gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata
    13081 atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg
    13141 ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc
    13201 cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc
    13261 attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa
    13321 ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc
    13381 acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg
    13441 ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg
    13501 ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct
    13561 ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt
    13621 atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc
    13681 tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa
    13741 aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc
    13801 tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg ctttggattg
    13861 atcaattctt tggcagtcag catgattgct gccttttatc acggagatga acgagcctct
    13921 atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca
    13981 ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct
    14041 attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat
    14101 ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt
    14161 tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg
    14221 acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg
    14281 gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg
    14341 cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac
    14401 ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct
    14461 tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca
    14521 atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc
    14581 tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg
    14641 ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata
    14701 agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt
    14761 aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc
    14821 cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac
    14881 tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa
    14941 ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa
    15001 catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta
    15061 ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt
    15121 ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc
    15181 gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact
    15241 gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta
    15301 gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat
    15361 gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg
    15421 aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt
    15481 caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat
    15541 tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat
    15601 ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa
    15661 tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta
    15721 gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc
    15781 ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc
    15841 atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta
    15901 gggtttattt atgggagagc aaaagattgg atatttattg attggtctga aatggataaa
    15961 gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact
    16021 tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat
    16081 caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt
    16141 ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt
    16201 ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc
    16261 ttattgaatg acactatcac acagattact actccatatt tcaaattcta tgaacaagat
    16321 gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga
    16381 gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga
    16441 aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga
    16501 gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt
    16561 tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta
    16621 cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct
    16681 gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag
    16741 ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca
    16801 tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg
    16861 cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata
    16921 atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc
    16981 ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag
    17041 cgattttaag atctcctaat actggaagga taaataatgg cagccaacta atcaataagc
    17101 tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac
    17161 cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca
    17221 agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta
    17281 atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg
    17341 gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa
    17401 ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga
    17461 ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag
    17521 caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa
    17581 agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact
    17641 ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat
    17701 tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg
    17761 ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta
    17821 ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga
    17881 atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca
    17941 aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa
    18001 ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac
    18061 tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt
    18121 ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa
    18181 cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta tccattgata
    18241 gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact
    18301 tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc
    18361 gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca
    18421 acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt
    18481 ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt
    18541 ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg
    18601 taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg
    18661 aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg
    18721 attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac
    18781 gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc
    18841 atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga
    18901 taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa
    18961 ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc
    19021 taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc
    19081 ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa
    19141 tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt
    19201 catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt
    19261 agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt
    19321 ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga
    19381 gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc
    19441 cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat
    19501 gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga
    19561 agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat
    19621 acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc
    19681 aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa
    19741 aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa
    19801 aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact
    19861 aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt
    19921 ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg
    19981 tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta
    20041 ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg
    20101 catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag
    20161 cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct
    20221 tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg
    20281 aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc
    20341 ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat
    20401 tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa
    20461 ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat
    20521 ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta
    20581 cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg
    20641 gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt
    20701 gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat
    20761 tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt
    20821 tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata
    20881 aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt
    20941 cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca
    21001 ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta
    21061 gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga
    21121 ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc
    21181 aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact
    21241 aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt
    21301 tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa
    21361 tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga
    21421 tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct
    21481 ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga
    21541 tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca
    21601 tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa
    21661 tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca
    21721 taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact
    21781 gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct
    21841 tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact
    21901 agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt
    21961 gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta
    22021 tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata
    22081 ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca
    22141 ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat
    22201 atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa
    22261 cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag
    22321 atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat
    22381 tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta
    22441 catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac
    22501 gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa
    22561 agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac
    22621 gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat
    22681 ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt
    22741 agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga
    22801 ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat
    22861 catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg
    22921 cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt
    22981 gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa
    23041 acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac
    23101 tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc
    23161 tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg
    23221 ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata
    23281 gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa
    23341 cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc
    23401 taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc
    23461 agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt
    23521 acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga
    23581 tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg
    23641 attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg
    23701 agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct
    23761 gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct
    23821 ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta
    23881 ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc
    23941 gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga
    24001 tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct
    24061 tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg
    24121 atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct
    24181 attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc
    24241 atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat
    24301 ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc
    24361 aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc
    24421 cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat
    24481 caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa
    24541 tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc
    24601 tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga
    24661 tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc
    24721 ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc
    24781 tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt
    24841 tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc
    24901 gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata
    24961 attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg
    25021 gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc
    25081 aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt
    25141 tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc
    25201 accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc
    25261 atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat
    25321 tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt
    25381 tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc
    25441 attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt
    25501 tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc
    25561 ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat
    25621 ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt
    25681 atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac
    25741 agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat
    25801 cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc
    25861 agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt
    25921 tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata
    25981 aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc
    26041 gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg
    26101 agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa
    26161 aataaggaaa aggactccta acataacgtt tattttttta tgatttttca ttttttcact
    26221 cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg
    26281 cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt
    26341 ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg
    26401 gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat
    26461 cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg
    26521 tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa
    26581 tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt
    26641 gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca
    26701 ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg
    26761 cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg
    26821 atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc
    26881 ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca
    26941 ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag
    27001 gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca
    27061 aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt
    27121 ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg
    27181 acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct
    27241 ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct
    27301 tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct
    27361 accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat
    27421 gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga
    27481 tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt
    27541 tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga
    27601 cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa
    27661 aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc
    27721 caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct
    27781 ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg
    27841 acccatcttc gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg
    27901 taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg
    27961 tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg
    28021 ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta
    28081 gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt
    28141 aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc
    28201 cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg
    28261 caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt
    28321 ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg
    28381 catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg
    28441 tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct
    28501 tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca
    28561 gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc
    28621 catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa
    28681 gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt
    28741 tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt
    28801 ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata
    28861 tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg
    28921 tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag
    28981 catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat
    29041 tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag
    29101 atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc
    29161 cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt
    29221 ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat
    29281 ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg
    29341 aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc
    29401 ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat
    29461 gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac
    29521 gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa
    29581 gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa
    29641 acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga
    29701 ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc
    29761 tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca
    29821 gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta
    29881 gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat
    29941 ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc
    30001 ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat
    30061 cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc
    30121 ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac
    30181 gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta
    30241 tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat
    30301 taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt
    30361 gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct
    30421 gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca
    30481 aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt
    30541 cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat
    30601 caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg
    30661 ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg
    30721 taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac
    30781 ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg
    30841 catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa
    30901 ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt
    30961 ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca
    31021 tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata
    31081 aacaggcgtt acataatttt atttatttta atatgataat tattatacga agggcgctaa
    31141 agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt
    31201 aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg
    31261 aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga
    31321 tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata
    31381 tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg
    31441 ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc
    31501 aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg
    31561 agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat
    31621 gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa
    31681 ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact
    31741 acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca
    31801 agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta
    31861 ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag
    31921 ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa
    31981 atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata
    32041 cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
    32101 atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg
    32161 ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt
    32221 tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg
    32281 ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt
    32341 atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt
    32401 ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag
    32461 aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg
    32521 aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca
    32581 ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag
    32641 aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc
    32701 ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca
    32761 atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca
    32821 atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct
    32881 tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc
    32941 attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt
    33001 aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa
    33061 tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct
    33121 tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga
    33181 aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg
    33241 acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc
    33301 tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg
    33361 attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc
    33421 tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc
    33481 atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac
    33541 gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct
    33601 tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc
    33661 tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt
    33721 tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt
    33781 taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc
    33841 tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga
    33901 agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg
    33961 accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga
    34021 gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt
    34081 catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa
    34141 aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac
    34201 caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa
    34261 agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg
    34321 atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt
    34381 gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc
    34441 atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca
    34501 atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt
    34561 attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc
    34621 aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca
    34681 ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca
    34741 attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat
    34801 tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta
    34861 acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct
    34921 cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt
    34981 gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct
    35041 tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata
    35101 ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac
    35161 atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt
    35221 aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag
    35281 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
    35341 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
    35401 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
    35461 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
    35521 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
    35581 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
    35641 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
    35701 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
    35761 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
    35821 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
    35881 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
    35941 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
    36001 attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
    36061 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
    36121 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
    36181 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
    36241 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
    36301 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
    36361 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
    36421 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
    36481 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
    36541 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
    36601 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
    36661 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
    36721 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
    36781 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
    36841 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
    36901 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
    36961 aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt
    37021 ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag
    37081 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
    37141 tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
    37201 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg
    37261 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
    37321 atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
    37381 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg
    37441 tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag
    37501 ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt
    37561 tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct
    37621 aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc
    37681 acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt
    37741 gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc
    37801 gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg
    37861 tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc
    37921 ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct
    37981 tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt
    38041 caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc
    38101 agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc
    38161 cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc
    38221 atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga
    38281 atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc
    38341 accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag
    38401 tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt
    38461 acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt
    38521 taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt
//
LOCUS       DACDBG010000020        37582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDBG010000020 DACDBG010000000
VERSION     DACDBG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.020"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IVZ90_000997"
     CDS             complement(151..2304)
                     /locus_tag="IVZ90_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8262064.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="IVZ90_000998"
     CDS             complement(2598..3446)
                     /locus_tag="IVZ90_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8262065.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IVZ90_000999"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IVZ90_000999"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8262066.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="IVZ90_001000"
     CDS             complement(5947..6126)
                     /locus_tag="IVZ90_001000"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8262067.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IVZ90_001001"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IVZ90_001001"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8262068.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="IVZ90_001002"
     CDS             8274..10553
                     /locus_tag="IVZ90_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8262069.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="IVZ90_001003"
     CDS             complement(10646..11293)
                     /locus_tag="IVZ90_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262070.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="IVZ90_001004"
     CDS             complement(11472..12191)
                     /locus_tag="IVZ90_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8262071.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="IVZ90_001005"
     CDS             complement(12322..13473)
                     /locus_tag="IVZ90_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8262072.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="IVZ90_001006"
     CDS             complement(13690..14430)
                     /locus_tag="IVZ90_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8262073.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IVZ90_001007"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IVZ90_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8262074.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="IVZ90_001008"
     CDS             complement(14814..15407)
                     /locus_tag="IVZ90_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8262075.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="IVZ90_001009"
     CDS             complement(15391..16038)
                     /locus_tag="IVZ90_001009"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8262076.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IVZ90_001010"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IVZ90_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8262077.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IVZ90_001011"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IVZ90_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8262078.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="IVZ90_001012"
     CDS             complement(17484..18011)
                     /locus_tag="IVZ90_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8262079.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="IVZ90_001013"
     CDS             complement(18191..18976)
                     /locus_tag="IVZ90_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8262080.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="IVZ90_001014"
     CDS             complement(18969..19838)
                     /locus_tag="IVZ90_001014"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8262081.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="IVZ90_001015"
     CDS             complement(19839..21218)
                     /locus_tag="IVZ90_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8262082.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IVZ90_001016"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IVZ90_001016"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8262083.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IVZ90_001017"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IVZ90_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8262084.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IVZ90_001018"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IVZ90_001018"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8262085.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IVZ90_001019"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IVZ90_001019"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8262086.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IVZ90_001020"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IVZ90_001020"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8262087.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="IVZ90_001021"
     CDS             complement(25176..25400)
                     /locus_tag="IVZ90_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8262088.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="IVZ90_001022"
     CDS             complement(25453..26415)
                     /locus_tag="IVZ90_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8262089.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="IVZ90_001023"
     CDS             complement(26425..26865)
                     /locus_tag="IVZ90_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8262090.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="IVZ90_001024"
     CDS             27171..28136
                     /locus_tag="IVZ90_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8262091.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="IVZ90_001025"
     CDS             complement(28193..28423)
                     /locus_tag="IVZ90_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8262092.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="IVZ90_001026"
     CDS             complement(28509..29396)
                     /locus_tag="IVZ90_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8262093.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="IVZ90_001027"
     CDS             complement(29485..30708)
                     /locus_tag="IVZ90_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8262094.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="IVZ90_001028"
     CDS             complement(30725..31804)
                     /locus_tag="IVZ90_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8262095.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="IVZ90_001029"
     CDS             complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="IVZ90_001029"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8262096.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33662..33928)
                     /locus_tag="IVZ90_001030"
     CDS             complement(33662..33928)
                     /locus_tag="IVZ90_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8262097.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34407..36641
                     /locus_tag="IVZ90_001031"
     CDS             34407..36641
                     /locus_tag="IVZ90_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8262098.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGISVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36789..37109
                     /locus_tag="IVZ90_001032"
     CDS             36789..37109
                     /locus_tag="IVZ90_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8262099.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37314..37538)
                     /locus_tag="IVZ90_001033"
     CDS             complement(37314..37538)
                     /locus_tag="IVZ90_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8262100.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT        10544 a         8128 c         6531 g        12379 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agacgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acacctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac gcgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ctgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atcaagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcccaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcagaaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac tagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 ataactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccttaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgtgatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag cccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccaaaataaa aagctactaa
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 attttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattgcc tctgctgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtctga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aaggtccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa gtcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataggtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagcta cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggcgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ctatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt ttatctattc ttactgacaa
    31861 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    31921 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcgta
    31981 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    32041 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    32101 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    32161 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    32221 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    32281 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    32341 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    32401 ccttcactga tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    32461 ttgagtgtag ctaccatagg gaacatgata cgtaagttac catgaacaga tgcacgaagc
    32521 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    32581 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    32641 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    32701 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    32761 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    32821 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    32881 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    32941 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    33001 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtaccaac gattgctgga
    33061 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    33121 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    33181 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    33241 gctaaaatac gtttagcaac atcacgaata tctgcagcac gctcttgcat ataagcatta
    33301 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    33361 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    33421 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    33481 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    33541 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    33601 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    33661 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    33721 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    33781 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    33841 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    33901 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    33961 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    34021 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    34081 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    34141 tttattttct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    34201 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    34261 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    34321 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    34381 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    34441 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    34501 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    34561 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    34621 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    34681 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    34741 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    34801 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    34861 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    34921 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    34981 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    35041 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    35101 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    35161 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    35221 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    35281 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    35341 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    35401 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    35461 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    35521 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    35581 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    35641 ttgaacaacg gacaggtatt tcagtgggtg aattaaaaga aaaagaacag acacaactga
    35701 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    35761 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    35821 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    35881 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    35941 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    36001 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    36061 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    36121 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    36181 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    36241 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    36301 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    36361 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    36421 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    36481 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    36541 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    36601 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    36661 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    36721 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    36781 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    36841 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    36901 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    36961 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    37021 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    37081 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    37141 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    37201 gaagcaactc cttcggaaat aagctaaaat tcacgaaaat tgcttttcaa atttttgtga
    37261 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    37321 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    37381 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    37441 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    37501 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    37561 atcatatgta cgcaaaataa tt
//
LOCUS       DACDBG010000021        37245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDBG010000021 DACDBG010000000
VERSION     DACDBG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.021"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..839
                     /locus_tag="IVZ90_001034"
     CDS             <1..839
                     /locus_tag="IVZ90_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8262101.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="IVZ90_001035"
     CDS             complement(1263..1433)
                     /locus_tag="IVZ90_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8262102.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="IVZ90_001036"
     CDS             complement(1627..1806)
                     /locus_tag="IVZ90_001036"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262103.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1816..2040)
                     /locus_tag="IVZ90_001037"
     CDS             complement(1816..2040)
                     /locus_tag="IVZ90_001037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262104.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2193..3305
                     /locus_tag="IVZ90_001038"
     CDS             2193..3305
                     /locus_tag="IVZ90_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8262105.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="IVZ90_001039"
     CDS             complement(3383..3676)
                     /locus_tag="IVZ90_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8262106.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="IVZ90_001040"
     CDS             3844..4158
                     /locus_tag="IVZ90_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8262107.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="IVZ90_001041"
     CDS             4179..6158
                     /locus_tag="IVZ90_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8262108.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="IVZ90_001042"
     CDS             6171..6497
                     /locus_tag="IVZ90_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8262109.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="IVZ90_001043"
     CDS             6513..7031
                     /locus_tag="IVZ90_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262110.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="IVZ90_001044"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="IVZ90_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8262111.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="IVZ90_001045"
     CDS             complement(8467..9972)
                     /locus_tag="IVZ90_001045"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8262112.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="IVZ90_001046"
     CDS             complement(10064..10654)
                     /locus_tag="IVZ90_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8262113.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="IVZ90_001047"
     CDS             10833..12479
                     /locus_tag="IVZ90_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8262114.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="IVZ90_001048"
     CDS             12528..13247
                     /locus_tag="IVZ90_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8262115.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="IVZ90_001049"
     CDS             13726..14673
                     /locus_tag="IVZ90_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8262116.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="IVZ90_001050"
     CDS             14685..15605
                     /locus_tag="IVZ90_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262117.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="IVZ90_001051"
     CDS             15646..17103
                     /locus_tag="IVZ90_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8262118.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="IVZ90_001052"
     CDS             17448..18062
                     /locus_tag="IVZ90_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8262119.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="IVZ90_001053"
     CDS             18055..19779
                     /locus_tag="IVZ90_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8262120.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="IVZ90_001054"
     CDS             19776..21227
                     /locus_tag="IVZ90_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262121.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="IVZ90_001055"
     CDS             complement(21700..23853)
                     /locus_tag="IVZ90_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8262122.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="IVZ90_001056"
     CDS             complement(23855..24901)
                     /locus_tag="IVZ90_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8262123.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="IVZ90_001057"
     CDS             25051..26340
                     /locus_tag="IVZ90_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8262124.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="IVZ90_001058"
     CDS             26353..29055
                     /locus_tag="IVZ90_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8262125.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="IVZ90_001059"
     CDS             29040..29897
                     /locus_tag="IVZ90_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8262126.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30825)
                     /locus_tag="IVZ90_001060"
     CDS             complement(29968..30825)
                     /locus_tag="IVZ90_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8262127.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31036..33207
                     /locus_tag="IVZ90_001061"
     CDS             31036..33207
                     /locus_tag="IVZ90_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8262128.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="IVZ90_001062"
     CDS             33275..33616
                     /locus_tag="IVZ90_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8262129.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="IVZ90_001063"
     CDS             complement(33892..35646)
                     /locus_tag="IVZ90_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262130.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="IVZ90_001064"
     CDS             complement(35657..36493)
                     /locus_tag="IVZ90_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8262131.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="IVZ90_001065"
     CDS             complement(36495..37178)
                     /locus_tag="IVZ90_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8262132.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6552 c         7638 g        11039 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DACDBG010000022        34330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDBG010000022 DACDBG010000000
VERSION     DACDBG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.022"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IVZ90_001066"
     CDS             202..1077
                     /locus_tag="IVZ90_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262133.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IVZ90_001067"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IVZ90_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IVZ90_001068"
     CDS             complement(1642..2973)
                     /locus_tag="IVZ90_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8262134.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IVZ90_001069"
     CDS             complement(3122..3835)
                     /locus_tag="IVZ90_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8262135.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IVZ90_001070"
     CDS             4010..5434
                     /locus_tag="IVZ90_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8262136.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IVZ90_001071"
     CDS             5733..6440
                     /locus_tag="IVZ90_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8262137.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IVZ90_001072"
     CDS             6427..6750
                     /locus_tag="IVZ90_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8262138.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IVZ90_001073"
     CDS             complement(6839..7876)
                     /locus_tag="IVZ90_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8262139.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IVZ90_001074"
     CDS             complement(8197..9180)
                     /locus_tag="IVZ90_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8262140.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IVZ90_001075"
     CDS             complement(9595..10365)
                     /locus_tag="IVZ90_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262141.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IVZ90_001076"
     CDS             complement(10358..11293)
                     /locus_tag="IVZ90_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262142.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IVZ90_001077"
     CDS             complement(11609..13258)
                     /locus_tag="IVZ90_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8262143.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IVZ90_001078"
     CDS             complement(13375..15978)
                     /locus_tag="IVZ90_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8262144.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IVZ90_001079"
     CDS             complement(15990..17039)
                     /locus_tag="IVZ90_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8262145.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IVZ90_001080"
     CDS             complement(17065..17379)
                     /locus_tag="IVZ90_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8262146.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IVZ90_001081"
     CDS             complement(17395..17841)
                     /locus_tag="IVZ90_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8262147.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="IVZ90_001082"
     CDS             complement(17842..19818)
                     /locus_tag="IVZ90_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8262148.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="IVZ90_001083"
     CDS             complement(19976..20872)
                     /locus_tag="IVZ90_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8262149.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="IVZ90_001084"
     CDS             complement(20865..21545)
                     /locus_tag="IVZ90_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262150.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="IVZ90_001085"
     CDS             complement(21546..22328)
                     /locus_tag="IVZ90_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8262151.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="IVZ90_001086"
     CDS             complement(22321..23238)
                     /locus_tag="IVZ90_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8262152.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="IVZ90_001087"
     CDS             complement(23402..23725)
                     /locus_tag="IVZ90_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262153.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="IVZ90_001088"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="IVZ90_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8262154.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="IVZ90_001089"
     CDS             27853..28167
                     /locus_tag="IVZ90_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8262155.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="IVZ90_001090"
     CDS             28180..28554
                     /locus_tag="IVZ90_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8262156.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="IVZ90_001091"
     CDS             28564..28899
                     /locus_tag="IVZ90_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8262157.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="IVZ90_001092"
     CDS             28982..30322
                     /locus_tag="IVZ90_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8262158.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="IVZ90_001093"
     CDS             30400..31074
                     /locus_tag="IVZ90_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8262159.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31307..31741
                     /gene="dcm"
                     /locus_tag="IVZ90_001094"
     CDS             31307..31741
                     /gene="dcm"
                     /locus_tag="IVZ90_001094"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8262160.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            31742..32449
                     /locus_tag="IVZ90_001095"
     CDS             31742..32449
                     /locus_tag="IVZ90_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8262161.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            32439..32729
                     /locus_tag="IVZ90_001096"
     CDS             32439..32729
                     /locus_tag="IVZ90_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262162.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="IVZ90_001097"
     CDS             32988..34172
                     /locus_tag="IVZ90_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8262163.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34187..34306
                     /locus_tag="IVZ90_001098"
     CDS             34187..34306
                     /locus_tag="IVZ90_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8262164.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10702 a         6614 c         6253 g        10761 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg cagaacaagt tttggataaa accatcgtct
    27061 tattcaacta tgtgtatgaa aacaagaaac cctatgaagc aggtgatgat ccagtagcga
    27121 aagatgctag cttgaacaat caagcccaaa ctgtcaattg tacggtggaa cgccatgttt
    27181 ccatccaaac aaaagcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DACDBG010000023        33095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDBG010000023 DACDBG010000000
VERSION     DACDBG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.023"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            150..299
                     /locus_tag="IVZ90_001099"
     CDS             150..299
                     /locus_tag="IVZ90_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8262165.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            310..1824
                     /gene="dltA"
                     /locus_tag="IVZ90_001100"
     CDS             310..1824
                     /gene="dltA"
                     /locus_tag="IVZ90_001100"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8262166.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1821..3026
                     /gene="dltB"
                     /locus_tag="IVZ90_001101"
     CDS             1821..3026
                     /gene="dltB"
                     /locus_tag="IVZ90_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8262167.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3071..3304
                     /gene="dltC"
                     /locus_tag="IVZ90_001102"
     CDS             3071..3304
                     /gene="dltC"
                     /locus_tag="IVZ90_001102"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8262168.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3307..4575
                     /gene="dltD"
                     /locus_tag="IVZ90_001103"
     CDS             3307..4575
                     /gene="dltD"
                     /locus_tag="IVZ90_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8262169.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4716..5798
                     /locus_tag="IVZ90_001104"
     CDS             4716..5798
                     /locus_tag="IVZ90_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8262170.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5798..6601
                     /locus_tag="IVZ90_001105"
     CDS             5798..6601
                     /locus_tag="IVZ90_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8262171.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6671..7411
                     /locus_tag="IVZ90_001106"
     CDS             6671..7411
                     /locus_tag="IVZ90_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8262172.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7422..8426
                     /locus_tag="IVZ90_001107"
     CDS             7422..8426
                     /locus_tag="IVZ90_001107"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8262173.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8471..10315)
                     /locus_tag="IVZ90_001108"
     CDS             complement(8471..10315)
                     /locus_tag="IVZ90_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262174.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10527..11045
                     /locus_tag="IVZ90_001109"
     CDS             10527..11045
                     /locus_tag="IVZ90_001109"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8262175.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11173..11856
                     /locus_tag="IVZ90_001110"
     CDS             11173..11856
                     /locus_tag="IVZ90_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8262176.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12165..12238
                     /locus_tag="IVZ90_001111"
     tRNA            12165..12238
                     /locus_tag="IVZ90_001111"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12199..12201,aa:Arg,seq:tct)
     gene            12397..12756
                     /locus_tag="IVZ90_001112"
     CDS             12397..12756
                     /locus_tag="IVZ90_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8262177.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            12879..14510
                     /locus_tag="IVZ90_001113"
     CDS             12879..14510
                     /locus_tag="IVZ90_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8262178.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(14572..15213)
                     /locus_tag="IVZ90_001114"
     CDS             complement(14572..15213)
                     /locus_tag="IVZ90_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8262179.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            15495..15803
                     /gene="rplU"
                     /locus_tag="IVZ90_001115"
     CDS             15495..15803
                     /gene="rplU"
                     /locus_tag="IVZ90_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8262180.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            15830..16174
                     /locus_tag="IVZ90_001116"
     CDS             15830..16174
                     /locus_tag="IVZ90_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8262181.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            16187..16480
                     /gene="rpmA"
                     /locus_tag="IVZ90_001117"
     CDS             16187..16480
                     /gene="rpmA"
                     /locus_tag="IVZ90_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8262182.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            16887..19721
                     /locus_tag="IVZ90_001118"
     CDS             16887..19721
                     /locus_tag="IVZ90_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8262183.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            19912..20403
                     /locus_tag="IVZ90_001119"
     CDS             19912..20403
                     /locus_tag="IVZ90_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8262184.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            20506..21495
                     /gene="manX"
                     /locus_tag="IVZ90_001120"
     CDS             20506..21495
                     /gene="manX"
                     /locus_tag="IVZ90_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8262185.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            21528..22331
                     /locus_tag="IVZ90_001121"
     CDS             21528..22331
                     /locus_tag="IVZ90_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8262186.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            22352..23266
                     /locus_tag="IVZ90_001122"
     CDS             22352..23266
                     /locus_tag="IVZ90_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8262187.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            23349..23723
                     /locus_tag="IVZ90_001123"
     CDS             23349..23723
                     /locus_tag="IVZ90_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8262188.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            23862..24107
                     /locus_tag="IVZ90_001124"
     CDS             23862..24107
                     /locus_tag="IVZ90_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8262189.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            24279..25343
                     /locus_tag="IVZ90_001125"
     CDS             24279..25343
                     /locus_tag="IVZ90_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8262190.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            25589..26014
                     /locus_tag="IVZ90_001126"
     CDS             25589..26014
                     /locus_tag="IVZ90_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8262191.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            26007..26456
                     /gene="nusB"
                     /locus_tag="IVZ90_001127"
     CDS             26007..26456
                     /gene="nusB"
                     /locus_tag="IVZ90_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8262192.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            26553..27407
                     /locus_tag="IVZ90_001128"
     CDS             26553..27407
                     /locus_tag="IVZ90_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8262193.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            27408..28745
                     /locus_tag="IVZ90_001129"
     CDS             27408..28745
                     /locus_tag="IVZ90_001129"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8262194.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            28750..28974
                     /locus_tag="IVZ90_001130"
     CDS             28750..28974
                     /locus_tag="IVZ90_001130"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8262195.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            28976..29866
                     /locus_tag="IVZ90_001131"
     CDS             28976..29866
                     /locus_tag="IVZ90_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8262196.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            29875..30693
                     /locus_tag="IVZ90_001132"
     CDS             29875..30693
                     /locus_tag="IVZ90_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8262197.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            30800..31249
                     /locus_tag="IVZ90_001133"
     CDS             30800..31249
                     /locus_tag="IVZ90_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8262198.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            31267..32949
                     /gene="recN"
                     /locus_tag="IVZ90_001134"
     CDS             31267..32949
                     /gene="recN"
                     /locus_tag="IVZ90_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8262199.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(33004..>33095)
                     /locus_tag="IVZ90_001135"
     CDS             complement(33004..>33095)
                     /locus_tag="IVZ90_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8262200.1"
                     /translation="FAKVTKFVIWTMLILTIGSVVLTAVMSVM"
BASE COUNT        10756 a         5480 c         7115 g         9744 t
ORIGIN      
        1 aagaaaaaag cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag
       61 taaaaaaacg gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt
      121 tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga
      181 attattgggg aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga
      241 tttatctgta tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg
      301 aggaagagta tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa
      361 cctgatcgag ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca
      421 tcagacgcac ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt
      481 tttggcaatt tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat
      541 gcctatttgc cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg
      601 gctaaaccat ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct
      661 gtcataacaa aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga
      721 aatagcgtaa aaggtacaga aaatttttat attattttta cttctggtac tactggagaa
      781 ccaaaaggcg tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa
      841 gactttggga tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta
      901 tcggtatttt ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa
      961 gcagttgttc aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg
     1021 tgggtttcta ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa
     1081 aatgtgccgg aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca
     1141 gaaagtctac ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa
     1201 gcaactgtag ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga
     1261 ctaccgattg gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct
     1321 gtttcaaaag gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac
     1381 ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac
     1441 catacagggg atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg
     1501 gatcagcagg tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg
     1561 ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta
     1621 cagcagctgg ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg
     1681 actaaatcta tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag
     1741 ttccattatg tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta
     1801 acagctgagg tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt
     1861 atttttttct tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa
     1921 aaagattacc agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag
     1981 gtaattattg gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg
     2041 tatggggcta ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg
     2101 ttattttagc gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa
     2161 aagcatcgtt acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga
     2221 tcatggaaat aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc
     2281 tgctgttctt tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag
     2341 atcagctcca tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa
     2401 atatttttgt cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc
     2461 ttcctgtagt gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt
     2521 atatgtacgt gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag
     2581 tcggtacaag ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat
     2641 catggaatat caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg
     2701 attatgtata tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga
     2761 tcgtaatgtc aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat
     2821 tgacgtggta ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg
     2881 catggatccg atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg
     2941 gcttagccgt atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat
     3001 ttttagataa actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga
     3061 gaaaaggaaa atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga
     3121 agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc
     3181 tgttcaatta ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt
     3241 cgagcgagag gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg
     3301 ttgagtatga aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta
     3361 ctgtttgtgt tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga
     3421 cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca
     3481 ttatctgaaa aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca
     3541 tttcatccgt ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga
     3601 gcacccggga cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg
     3661 aaaaacaaga aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa
     3721 actgattact ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg
     3781 aagaaagtaa cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta
     3841 aaagagaatg atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta
     3901 cctgaatctt tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta
     3961 ttcagcaata ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta
     4021 ccaaaacaat accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa
     4081 acgacgaata atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac
     4141 gagccagagc tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct
     4201 gattttcagc tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc
     4261 ccaccggtta acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga
     4321 tttgccaaaa agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt
     4381 gtcaatcaag cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc
     4441 tggttggctg cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt
     4501 aagtatcatc ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa
     4561 ctacaattaa aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat
     4621 attctgaata aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat
     4681 aatcacaata tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac
     4741 ttattgattt aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat
     4801 tcagagaata tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga
     4861 ttgccaaaca tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag
     4921 ttggttttat ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag
     4981 gttggtgggg gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa
     5041 tcgtccacgg tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata
     5101 aaccttatga aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca
     5161 aagagtgggg catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga
     5221 atggcagtaa atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc
     5281 tgcgagtatt agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg
     5341 atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg
     5401 atattgcttt tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag
     5461 aagcagattc gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc
     5521 cgcataaaaa attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc
     5581 aatatacagt gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg
     5641 gtgttccgtc tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga
     5701 ttcatgaaga tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg
     5761 atagtgaaac agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc
     5821 tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct
     5881 tgttttctta tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc
     5941 ttttccggaa gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt
     6001 attcgcattc aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc
     6061 aaaaagtaac cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat
     6121 caatttgatc aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt
     6181 tttaatcagc ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac
     6241 gggtgtcatg ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat
     6301 ttttgtttca gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac
     6361 aaagttcgac tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt
     6421 gaaagataga attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct
     6481 aggagcaatc gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt
     6541 tgcagaattt tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta
     6601 gcatgtaagc agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg
     6661 ggaatgtacg atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca
     6721 agggacaaca ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag
     6781 tgctacgctg atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct
     6841 tagccattat catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact
     6901 tcatcctaag agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat
     6961 tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga
     7021 agaattaaaa attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg
     7081 ttacgccatg cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc
     7141 aggttatttg gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac
     7201 ctatcttttt gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga
     7261 acttgcgaaa tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga
     7321 tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact
     7381 tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg
     7441 aaaaccacga cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt
     7501 tagatgaacc tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc
     7561 aaaatacgct tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc
     7621 gtcgcttaag tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta
     7681 tcatgcctga tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc
     7741 aagcacttca tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca
     7801 ttgatggcat gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc
     7861 atggaacaat tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa
     7921 aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac
     7981 catttcttcc agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt
     8041 tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag
     8101 attggaaaaa aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg
     8161 ggaagtctcc agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag
     8221 tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg
     8281 gactaggaga tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat
     8341 ctctatcaaa aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag
     8401 aagaattgct taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt
     8461 ccgctatttt ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa
     8521 tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca
     8581 tcattaagtc aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt
     8641 ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta
     8701 tcgacaaata aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat
     8761 gctctaaagc gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg
     8821 gaagatctgc ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg
     8881 ctaagataag atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg
     8941 cttcttctaa tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat
     9001 cttgcagatt ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat
     9061 gctgtcgaag ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta
     9121 cttgtctccg atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa
     9181 cgattagaat gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta
     9241 atgccgtttg gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac
     9301 aagctacata aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta
     9361 aagtgcttat aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag
     9421 taaaagttgc ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc
     9481 cgtaccattt tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg
     9541 ttaagataat aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac
     9601 ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa
     9661 taagtgagta ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc
     9721 ctatagcgat aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa
     9781 taaaactaat caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt
     9841 tctgataagg agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat
     9901 ctatcacagc caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata
     9961 aaatcttttc gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg
    10021 cttcgtccaa cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg
    10081 taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga
    10141 aaacccacga atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat
    10201 aaaagaaata agcaattcct gttaaaaata acaatgataa tataatgatt atgctccact
    10261 ttatacgtaa ataaaaagat tcttttttat tatatatgtt attaaaaaaa gacataaagg
    10321 tattcctttc taaaacttgt aatgttaggg taaacgtttt ttaaactaac gtcaattatt
    10381 tataataaaa aatgagataa agatgaggaa atagaattag aaacatataa ttattaaagg
    10441 gaaacatttt tacagctata atttgaaaaa aatttatcgt tattatatga ttaagaagga
    10501 ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt
    10561 ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt
    10621 ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa
    10681 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
    10741 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
    10801 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
    10861 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
    10921 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
    10981 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
    11041 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
    11101 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
    11161 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
    11221 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
    11281 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
    11341 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
    11401 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
    11461 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
    11521 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
    11581 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
    11641 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
    11701 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
    11761 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
    11821 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
    11881 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
    11941 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
    12001 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
    12061 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
    12121 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
    12181 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
    12241 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
    12301 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
    12361 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
    12421 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
    12481 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
    12541 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
    12601 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
    12661 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
    12721 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
    12781 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
    12841 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
    12901 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
    12961 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
    13021 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
    13081 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
    13141 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
    13201 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
    13261 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
    13321 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
    13381 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
    13441 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
    13501 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
    13561 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
    13621 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
    13681 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
    13741 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
    13801 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
    13861 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
    13921 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
    13981 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
    14041 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
    14101 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
    14161 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
    14221 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
    14281 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
    14341 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
    14401 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
    14461 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
    14521 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
    14581 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
    14641 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
    14701 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
    14761 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
    14821 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
    14881 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
    14941 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
    15001 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
    15061 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
    15121 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
    15181 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
    15241 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
    15301 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
    15361 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
    15421 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
    15481 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
    15541 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
    15601 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
    15661 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
    15721 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
    15781 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
    15841 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
    15901 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
    15961 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
    16021 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
    16081 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
    16141 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
    16201 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
    16261 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
    16321 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
    16381 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
    16441 aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga
    16501 ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga
    16561 ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg
    16621 atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat
    16681 aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt
    16741 ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag
    16801 gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa
    16861 tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg
    16921 tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg
    16981 aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag
    17041 tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac
    17101 tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct
    17161 ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag
    17221 ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa
    17281 gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg
    17341 cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt
    17401 tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg
    17461 caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg
    17521 gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct
    17581 tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag
    17641 caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt
    17701 ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg
    17761 agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga
    17821 tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag
    17881 gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat
    17941 cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc
    18001 aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa
    18061 ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata
    18121 atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag
    18181 aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag
    18241 accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca
    18301 tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc
    18361 aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag
    18421 gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat
    18481 atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag
    18541 aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg
    18601 gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag
    18661 ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg
    18721 atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg
    18781 gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag
    18841 acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg
    18901 tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt
    18961 ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata
    19021 agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag
    19081 aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt
    19141 cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta
    19201 ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg
    19261 atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg
    19321 aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata
    19381 acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa
    19441 tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc
    19501 acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg
    19561 aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt
    19621 tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag
    19681 tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac
    19741 ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc
    19801 gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca
    19861 cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt
    19921 cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag
    19981 caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga
    20041 aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag
    20101 aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc
    20161 accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat
    20221 atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat
    20281 caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga
    20341 aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg
    20401 tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta
    20461 atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc
    20521 ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc
    20581 gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga
    20641 gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt
    20701 gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat
    20761 aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc
    20821 ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt
    20881 gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac
    20941 gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat
    21001 gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa
    21061 gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt
    21121 aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc
    21181 aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt
    21241 gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat
    21301 gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga
    21361 caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt
    21421 aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa
    21481 ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt
    21541 caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat
    21601 ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg
    21661 aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg
    21721 gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt
    21781 taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg
    21841 ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga
    21901 tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg
    21961 tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac
    22021 aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg
    22081 gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag
    22141 aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga
    22201 ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg
    22261 gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga
    22321 atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa
    22381 aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga
    22441 acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa
    22501 aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc
    22561 atacattgct tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg
    22621 cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc
    22681 tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc
    22741 ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat
    22801 tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat
    22861 tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg
    22921 gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt
    22981 atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg
    23041 tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa
    23101 ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt
    23161 aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg
    23221 tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata
    23281 gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga
    23341 gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt
    23401 ttacgggtct gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc
    23461 atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag
    23521 catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg
    23581 gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg
    23641 tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc
    23701 gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat
    23761 ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat
    23821 cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat
    23881 tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga
    23941 tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt
    24001 tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag
    24061 tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat
    24121 agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa
    24181 taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat
    24241 aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat
    24301 tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc
    24361 ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag
    24421 cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg
    24481 aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag
    24541 aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag
    24601 aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag
    24661 tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat
    24721 atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac
    24781 tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta
    24841 gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg
    24901 acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta
    24961 cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg
    25021 ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag
    25081 ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac
    25141 atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat
    25201 ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag
    25261 tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac
    25321 caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt
    25381 tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa
    25441 gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg
    25501 gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc
    25561 gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc
    25621 aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat
    25681 tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac
    25741 tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg
    25801 cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat
    25861 ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga
    25921 agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga
    25981 actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg
    26041 aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg
    26101 cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt
    26161 ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg
    26221 ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg
    26281 atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg
    26341 ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta
    26401 aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc
    26461 tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa
    26521 tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat
    26581 tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta
    26641 tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa
    26701 gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg
    26761 agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt
    26821 ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt
    26881 tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc
    26941 ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag
    27001 cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg
    27061 gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta
    27121 aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac
    27181 gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga
    27241 tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta
    27301 ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga
    27361 tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat
    27421 atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt
    27481 tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc
    27541 aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat
    27601 tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt
    27661 cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa
    27721 taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt
    27781 ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc
    27841 caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc
    27901 aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga
    27961 atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg
    28021 gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag
    28081 ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag
    28141 tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt
    28201 cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc
    28261 agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc
    28321 ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc
    28381 aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat
    28441 tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag
    28501 aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc
    28561 aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct
    28621 atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc
    28681 tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag
    28741 aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc
    28801 atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga
    28861 atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa
    28921 atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag
    28981 attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt
    29041 agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg
    29101 cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat
    29161 tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat
    29221 ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca
    29281 taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt
    29341 ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt
    29401 agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga
    29461 atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt
    29521 gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt
    29581 gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag atgatctact
    29641 cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga
    29701 aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga
    29761 gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg
    29821 cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa
    29881 aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc
    29941 aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg
    30001 ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt
    30061 tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga
    30121 aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat
    30181 ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga
    30241 caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac
    30301 tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg
    30361 atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa
    30421 ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag
    30481 acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg
    30541 ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac
    30601 ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt
    30661 tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa
    30721 gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat
    30781 gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt
    30841 gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat
    30901 atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc
    30961 ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac
    31021 taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt
    31081 tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta
    31141 caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac
    31201 tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg
    31261 aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt
    31321 tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt
    31381 atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga
    31441 gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa
    31501 ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg
    31561 tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg
    31621 agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc
    31681 cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag
    31741 aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa
    31801 gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa
    31861 gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa
    31921 aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg
    31981 aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc
    32041 gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg
    32101 ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt
    32161 cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc
    32221 gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct
    32281 gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta
    32341 cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa
    32401 aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt
    32461 ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac
    32521 attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa
    32581 ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt
    32641 attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat
    32701 aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt
    32761 gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa
    32821 acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct
    32881 ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa
    32941 aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta
    33001 tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat
    33061 tgtccaaatg acaaattttg ttactttagc aaaag
//
LOCUS       DACDBG010000024        31814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDBG010000024 DACDBG010000000
VERSION     DACDBG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.024"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            355..1965
                     /locus_tag="IVZ90_001136"
     CDS             355..1965
                     /locus_tag="IVZ90_001136"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8262201.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2257..3126
                     /locus_tag="IVZ90_001137"
     CDS             2257..3126
                     /locus_tag="IVZ90_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8262202.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3291..4562
                     /locus_tag="IVZ90_001138"
     CDS             3291..4562
                     /locus_tag="IVZ90_001138"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8262203.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4559..5854
                     /locus_tag="IVZ90_001139"
     CDS             4559..5854
                     /locus_tag="IVZ90_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8262204.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5953..6216
                     /locus_tag="IVZ90_001140"
     CDS             5953..6216
                     /locus_tag="IVZ90_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8262205.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6661..7110
                     /locus_tag="IVZ90_001141"
     CDS             6661..7110
                     /locus_tag="IVZ90_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8262206.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7167..8585
                     /locus_tag="IVZ90_001142"
     CDS             7167..8585
                     /locus_tag="IVZ90_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8262207.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8585..9445
                     /locus_tag="IVZ90_001143"
     CDS             8585..9445
                     /locus_tag="IVZ90_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8262208.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9619..10392
                     /locus_tag="IVZ90_001144"
     CDS             9619..10392
                     /locus_tag="IVZ90_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8262209.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10539..10904
                     /locus_tag="IVZ90_001145"
     CDS             10539..10904
                     /locus_tag="IVZ90_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8262210.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11052..12260)
                     /locus_tag="IVZ90_001146"
     CDS             complement(11052..12260)
                     /locus_tag="IVZ90_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262211.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12279..12557)
                     /locus_tag="IVZ90_001147"
     CDS             complement(12279..12557)
                     /locus_tag="IVZ90_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262212.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12750..13406
                     /locus_tag="IVZ90_001148"
     CDS             12750..13406
                     /locus_tag="IVZ90_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8262213.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13848..14645)
                     /locus_tag="IVZ90_001149"
     CDS             complement(13848..14645)
                     /locus_tag="IVZ90_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8262214.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14642..15826)
                     /locus_tag="IVZ90_001150"
     CDS             complement(14642..15826)
                     /locus_tag="IVZ90_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8262215.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15851..17098)
                     /locus_tag="IVZ90_001151"
     CDS             complement(15851..17098)
                     /locus_tag="IVZ90_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8262216.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17313..19058
                     /gene="dnaX"
                     /locus_tag="IVZ90_001152"
     CDS             17313..19058
                     /gene="dnaX"
                     /locus_tag="IVZ90_001152"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8262217.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19078..19392
                     /locus_tag="IVZ90_001153"
     CDS             19078..19392
                     /locus_tag="IVZ90_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8262218.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19417..20013
                     /gene="recR"
                     /locus_tag="IVZ90_001154"
     CDS             19417..20013
                     /gene="recR"
                     /locus_tag="IVZ90_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8262219.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20077..20721
                     /locus_tag="IVZ90_001155"
     CDS             20077..20721
                     /locus_tag="IVZ90_001155"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8262220.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20736..21065
                     /locus_tag="IVZ90_001156"
     CDS             20736..21065
                     /locus_tag="IVZ90_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262221.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21079..22017
                     /gene="holB"
                     /locus_tag="IVZ90_001157"
     CDS             21079..22017
                     /gene="holB"
                     /locus_tag="IVZ90_001157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8262222.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22053..22877
                     /locus_tag="IVZ90_001158"
     CDS             22053..22877
                     /locus_tag="IVZ90_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8262223.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22870..23217
                     /locus_tag="IVZ90_001159"
     CDS             22870..23217
                     /locus_tag="IVZ90_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8262224.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23286..24158
                     /gene="rsmI"
                     /locus_tag="IVZ90_001160"
     CDS             23286..24158
                     /gene="rsmI"
                     /locus_tag="IVZ90_001160"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8262225.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24267..25388
                     /gene="dinB"
                     /locus_tag="IVZ90_001161"
     CDS             24267..25388
                     /gene="dinB"
                     /locus_tag="IVZ90_001161"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8262226.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25442..26044)
                     /gene="nrdG"
                     /locus_tag="IVZ90_001162"
     CDS             complement(25442..26044)
                     /gene="nrdG"
                     /locus_tag="IVZ90_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8262227.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26359..28548)
                     /gene="nrdD"
                     /locus_tag="IVZ90_001163"
     CDS             complement(26359..28548)
                     /gene="nrdD"
                     /locus_tag="IVZ90_001163"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8262228.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28874..29641
                     /locus_tag="IVZ90_001164"
     CDS             28874..29641
                     /locus_tag="IVZ90_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262229.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29631..31673
                     /locus_tag="IVZ90_001165"
     CDS             29631..31673
                     /locus_tag="IVZ90_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262230.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        10230 a         5677 c         6656 g         9251 t
ORIGIN      
        1 tttatctatt atttcactta aaatttttca gtaacgattt tctgatttgc ttaggtttaa
       61 cgttagctat gtttatatca agcttattat gtgggttaaa ttttttttgc actcataaag
      121 gacttaagaa ataatttctc aaaggatatc gaaaaagaaa aaattgtaag cggatagtat
      181 tcatttgagt acttgacttt cccttagtca gcttgtatta tacaccttgg gcaccctttt
      241 agagggtcag aagtttacag aatttcggct ccctattctt attgaagaat agggagcttt
      301 gtttattgtc tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca
      361 aaatatattt ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct
      421 tctttaggac gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca
      481 tacatcaacg tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca
      541 gatgatggtg ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat
      601 ttgaacaaat actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa
      661 cgtaagggcg aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc
      721 aaagaaaaaa tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta
      781 gggggaacag taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa
      841 gcagatgtcg gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa
      901 gctgctggag aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta
      961 ggtatccaac cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa
     1021 aacaaattgg cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta
     1081 gatacacttt actccatccc attagtttta caagcacaag gaatggatca aattgtttgc
     1141 gaccatttaa aactagatac accagaagca gacatgacag aatggaaaga attagaagaa
     1201 cgtgtcttga acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta
     1261 ccagatgcat atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca
     1321 gacatcgaga tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt
     1381 ttgaaagacg cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg
     1441 aaaatcgaag cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt
     1501 ggtatgcaga tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca
     1561 tcagctgaaa cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa
     1621 aacatcgaga atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa
     1681 ggaacgaaga cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac
     1741 cgctatgagt tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca
     1801 ggtgtctcac cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt
     1861 gtgggatgcc aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc
     1921 aaagcatttg tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata
     1981 gaaagagaaa agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc
     2041 taatttgtta ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc
     2101 gctattggat attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt
     2161 tagtagaaaa ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac
     2221 aaatttagtt ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa
     2281 tttttaaaag ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg
     2341 gaatggactc aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa
     2401 acatcaatgg gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact
     2461 gatttagttg aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat
     2521 tacgaagctg ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca
     2581 catctaccat ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca
     2641 aaaggtatct cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc
     2701 ggtaacggcg aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac
     2761 tacttagctt gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta
     2821 gcttttgacc acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta
     2881 catggcggat caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca
     2941 aaagtaaatg ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc
     3001 gaagctggta aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt
     3061 aaagcagcaa tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa
     3121 gcgtaagcat ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc
     3181 tctccattct tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat
     3241 aaaatacaag catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa
     3301 tcgtaatcaa aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata
     3361 gtgcagtcgc attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg
     3421 tgccggacat acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg
     3481 tccatttttc caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc
     3541 cgcacggaaa aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta
     3601 aatttggcga agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg
     3661 acctacacgt caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca
     3721 tgtatttacg gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt
     3781 cagtcggagc aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa
     3841 ttgaaaacgc tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg
     3901 gggcaaaagt tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac
     3961 atggctgtcg gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag
     4021 cagctgcagt aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct
     4081 ttatagcaaa attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag
     4141 tttacccctc gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg
     4201 ccacagatct gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa
     4261 tcatcgatac gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg
     4321 ctgatattac cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca
     4381 cagaagtcgt ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg
     4441 cagaagggac aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta
     4501 tcgagaaact aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat
     4561 gagcgattat ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta
     4621 tgccaaagaa tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc
     4681 tgtgattcgt gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat
     4741 cgttggtcaa gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga
     4801 tatctatatt tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc
     4861 aggaaaagcc cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag
     4921 cgttaatgga aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc
     4981 tctttatccg caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat
     5041 gatcgatatc ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa
     5101 agcagggaaa acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga
     5161 agtagaatta atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg
     5221 aagcgtgaaa ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg
     5281 agtttctgaa ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt
     5341 tatcttgatg gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag
     5401 cggacgtaca ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt
     5461 cggggcagca agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt
     5521 cgatacagga agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct
     5581 agaattacaa ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa
     5641 aaaatcaggt acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg
     5701 gaaacttcga aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt
     5761 tttgcgtaaa acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg
     5821 taaaaaaaat ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa
     5881 gatgttaatg ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg
     5941 agcgaaaaaa tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat
     6001 tcaacaactg gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa
     6061 tgggaagacg gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca
     6121 ttctacactg gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa
     6181 aaatacggtc tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt
     6241 tagcagacta tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc
     6301 gtttgatgct gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata
     6361 gttttaatta tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc
     6421 gaagtaaaaa accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt
     6481 tgttttgtta tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac
     6541 acgcggatga tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga
     6601 tgctatactg acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt
     6661 atgaggtttg atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca
     6721 aaaggatcat tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat
     6781 gttcaaaaag tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa
     6841 gatggatttg ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt
     6901 tttcatacag ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt
     6961 tttgccatcg tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc
     7021 tccagacgat tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca
     7081 gatatcatca gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat
     7141 agtgaagaaa aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg
     7201 gaattgctca tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc
     7261 atcaagcaaa attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa
     7321 tcaaagaggc agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg
     7381 ccgggaaaaa agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa
     7441 tagacgaagc attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg
     7501 caaatgagaa aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc
     7561 catttgtgat cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg
     7621 ctgatcccaa ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag
     7681 atacagcgtt tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg
     7741 gaaaagcagg ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag
     7801 gttttcttgg cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa
     7861 ataaaattag cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt
     7921 tatcgatctt aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg
     7981 tgttggatgc tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg
     8041 gattacttat cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt
     8101 cgactttctc gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg
     8161 ctgcaggaat gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat
     8221 ttaccaaaga agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta
     8281 cagaaggtgc tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga
     8341 caggttcagc agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc
     8401 atggagggat ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct
     8461 tggcaattgc atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag
     8521 tgaaagagaa aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag
     8581 cgtaatgtta tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt
     8641 accagccttc aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag
     8701 attagaagca ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga
     8761 gtttgttgcg gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt
     8821 ggatcatggc ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt
     8881 gatgatcgat gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt
     8941 tgcgttagct catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa
     9001 tggttctgct gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat
     9061 ttttgtgaaa gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt
     9121 gtatccgaaa aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca
     9181 aatcgatctt ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga
     9241 agcagtaaaa tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt
     9301 caatgctttg agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta
     9361 tccagcagct aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac
     9421 agtaggcaag gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata
     9481 gtcttgtcat accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc
     9541 ttcttatttc tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa
     9601 agtaaatggg gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat
     9661 tatctaaaaa acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc
     9721 gaagccacga ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt
     9781 cgcggtggcg ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc
     9841 accaaattag aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt
     9901 cgagatgata tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac
     9961 ctagcaagat ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg
    10021 agtgaatata caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca
    10081 acaatcaacg gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata
    10141 atgagttgtc gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca
    10201 atggtcaaaa aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa
    10261 aaaattgcac ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg
    10321 acaagtaaag aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt
    10381 ctgtacggat aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc
    10441 ttatctttgc tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat
    10501 aatcttactt ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg
    10561 tagtgaaggc aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg
    10621 ttagctttat ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag
    10681 gggatctgtt gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt
    10741 cagctgcagt tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga
    10801 tgaaagtatt gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt
    10861 tgctaagtca aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta
    10921 ttcttatcga taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa
    10981 actattttta ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct
    11041 attcatcaag atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga
    11101 ctatgttttc ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca
    11161 atcacataca atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca
    11221 acgcctaagc ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt
    11281 tcgcggacga tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg
    11341 ccgactaatg ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat
    11401 aatcctgctg ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg
    11461 aaaatccttc cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa
    11521 aatgacccag cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca
    11581 cttgaataag aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca
    11641 ataaaactat tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata
    11701 ttttttacag gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac
    11761 cagataatga aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca
    11821 gcggcagcta aactagtact taatccataa taattgatgc cttccccttt tttagatgca
    11881 gggatatatg cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca
    11941 ttcaagaatc gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt
    12001 gaaaggagat agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac
    12061 tgtttcccca tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa
    12121 cctgcttggc tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc
    12181 aacagataat aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta
    12241 ttaaataatt tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat
    12301 ttttgtattt gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga
    12361 ataaataaat ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga
    12421 ttctcatcca ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact
    12481 aaagaagctt tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg
    12541 ttcatccagt aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata
    12601 acgaataaaa aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt
    12661 aatatcatta gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt
    12721 tagatgaaag tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg
    12781 ataaaaatga ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga
    12841 tggaagattc ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa
    12901 atgatgggag agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa
    12961 aagacgaata ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg
    13021 cagagtttta tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc
    13081 tacaacacta tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc
    13141 agcaaatgct attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg
    13201 atttattcgg tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag
    13261 aaatcgccaa attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac
    13321 tgaaaaaaga tggtattatc accgttaaag accatcatat cgttattttg aatatggact
    13381 tgttagaaga caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc
    13441 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa
    13501 taccgagggt cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact
    13561 ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt
    13621 gtgaccgagg agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg
    13681 catcaagtaa taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac
    13741 ttatcaccgt agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca
    13801 cgatttttgt cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg
    13861 ccgccaccgc caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt
    13921 ccaggaggtg ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg
    13981 gttttttcag ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat
    14041 ttcaattgat aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa
    14101 acaacagcaa tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt
    14161 tcttcgtcta cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta
    14221 gacaggtaag cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca
    14281 tctaagatcg aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa
    14341 atgtagattt cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg
    14401 actgcgtctc ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc
    14461 gttacgatca agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa
    14521 gattggattt gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca
    14581 tgagtagcca ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc
    14641 atcatatcaa gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag
    14701 cggtcagtcc caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc
    14761 cattcattgc gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc
    14821 ataccggcag taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac
    14881 cggtcaatgt tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta
    14941 attcagattg aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga
    15001 acccggctaa atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc
    15061 cttcgaccat cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag
    15121 aaagcttgcc tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta
    15181 tccgccatat gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg
    15241 tagcccaata agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag
    15301 ctttgggaac aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt
    15361 tctcgacagc aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata
    15421 gtacgacggg attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga
    15481 tctctgcacc acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt
    15541 cgtccgctgt atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca
    15601 tatcggcaag ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt
    15661 tgctttcttc gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt
    15721 cttctgtata gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag
    15781 tcaacggacc gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt
    15841 tctaaataaa ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt
    15901 tagggatgcc gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct
    15961 taggctgaac gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat
    16021 tttctgtccc gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat
    16081 tttgtttttc tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat
    16141 aagaaccatt tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt
    16201 cattttttcc ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta
    16261 cgctaggatc acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat
    16321 cggtgtaaga aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc
    16381 aagaatcatc caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat
    16441 aagaaatccg ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga
    16501 attcagctaa atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga
    16561 ttgcgtttgt ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc
    16621 gtaagaataa ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga
    16681 atttgattcc ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga
    16741 cgccgccaaa tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga
    16801 acatggaagg agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga
    16861 tgatttttcc accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc
    16921 cgtctgttaa aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga
    16981 ctttgacacc ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta
    17041 gccattgatc tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa
    17101 aaaattcctc ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag
    17161 gaagatgtga aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta
    17221 aatacctgaa aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga
    17281 gatagtgaat tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct
    17341 ggcgttcgca gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa
    17401 atgctattgt ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg
    17461 gaaagacgag tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg
    17521 gtgagccatg caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg
    17581 tgatcgaaat cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc
    17641 gggcaaatta tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata
    17701 tgctttcaac aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg
    17761 tcatattcat cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga
    17821 ctcagcgatt tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta
    17881 tcttgaaaga atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg
    17941 cagaaggcgg gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag
    18001 gaacaattac tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg
    18061 acagttactt gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc
    18121 aaattctcgc agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc
    18181 gtgacttgct gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca
    18241 gaagtacatt tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa
    18301 agattttgag cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg
    18361 aagtcgctac agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag
    18421 catttgcaga tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg
    18481 aaatcaagga actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc
    18541 aaacaaatca taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag
    18601 ttctgcggga agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc
    18661 tgatgagtct atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg
    18721 gacctacagg actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg
    18781 acgaagaatt gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc
    18841 ctgatcctgt ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc
    18901 aggcacaaga agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg
    18961 agttgcttcc tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac
    19021 tgtttggctc cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg
    19081 atgcgtggaa tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa
    19141 atggcagaag cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa
    19201 ttggtcaaag cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa
    19261 gtcgttgatc ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat
    19321 gcgatcgcac aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg
    19381 ccaggatttt agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg
    19441 aaactgatcg acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc
    19501 gccttttata cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta
    19561 agtgtaaaac gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc
    19621 tgcgaaatat gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg
    19681 aaagatgtaa tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat
    19741 ggtgtgttat caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg
    19801 cagcggctgc atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag
    19861 ggagaagcaa cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact
    19921 cgtctagcac atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta
    19981 ttgaaagctg tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata
    20041 caatataaaa gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc
    20101 gaggggcctg atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa
    20161 gaagtagcaa aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa
    20221 gagatccgag cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca
    20281 ctgctttatg cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca
    20341 aaagggaaaa tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca
    20401 ggccgacgca tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact
    20461 acgccggatt ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag
    20521 aaaaacagac aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt
    20581 gtacgtcatg cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat
    20641 gcaagaaaaa gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat
    20701 ccgcaatttt ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc
    20761 aagacaaaga cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa
    20821 aactatcatc gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg
    20881 aagatgaccg tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc
    20941 aatttgtatc tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag
    21001 tagaagtaga agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat
    21061 tttaggagga gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg
    21121 tatcagcagc ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa
    21181 ggggagaaag gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt
    21241 acccaaatga aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag
    21301 aaagaacatc cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag
    21361 atcagacgtt tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt
    21421 ttgatcaaag aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg
    21481 gaagagcccc ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc
    21541 cctactattc aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg
    21601 atccatcgat tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta
    21661 acaaacagtt taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag
    21721 gacttgattt atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat
    21781 gtccaaaaaa aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct
    21841 gtcttgatgt tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc
    21901 aaagtgaaac agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc
    21961 agcaatgtca gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt
    22021 gccgcaaagg atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata
    22081 gagatgcagg tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca
    22141 aggtattagt cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag
    22201 agattgatcc caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag
    22261 ctgaattaga aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa
    22321 aagaaaaagt gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg
    22381 atcgaaataa attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg
    22441 taaaagatct ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg
    22501 atgaagcaaa gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt
    22561 ttttaggcga tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga
    22621 atccaacaaa aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg
    22681 aagaatacga gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg
    22741 atggtaaagg gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga
    22801 ctggacggga aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag
    22861 gtgagatgaa tggataagag atcattatac gacggattga atcaattaga atcagagttg
    22921 agaaatacat taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa
    22981 aatacaactt tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa
    23041 gttgcacaag ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt
    23101 gaaaaaattt atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac
    23161 aatgacgagc cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca
    23221 ataacaagag gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt
    23281 gacagatgta taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg
    23341 taccgacgcc aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag
    23401 aatccgatct tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg
    23461 aaatccatac accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat
    23521 tgattgcttt gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt
    23581 ccatcagcga tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga
    23641 tctctatccc aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc
    23701 aaccattttt attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga
    23761 aaaagctaaa agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg
    23821 ctacattgac gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat
    23881 tgacaaaggt ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg
    23941 aagaacatcc agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc
    24001 cagtgacgga agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg
    24061 cttcaggcga aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa
    24121 aacaagaagt ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat
    24181 gaaagactaa tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt
    24241 tcttgtataa tgaataagag gtgaggatga tggagctgca atttccttta cataatgata
    24301 catcaagaaa aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag
    24361 atcatcctga gtttcgcgga catccattag taattgccag acaccccagt gacactggtg
    24421 ggcgtggagt agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa
    24481 tgagtgcaca aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc
    24541 ataaatacag cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg
    24601 tggaaccagt tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca
    24661 agtcagccat caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa
    24721 catgttctgc gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa
    24781 aacctagagg aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc
    24841 caattgaaaa atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga
    24901 tttatacagg aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa
    24961 aaatgggcta ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta
    25021 caagagagcg aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag
    25081 aagcagtgct gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag
    25141 tccaaaaaca tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga
    25201 ccaaaagagt gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa
    25261 gccttatatg ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt
    25321 tgacgaatct ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag
    25381 aaatataaaa acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt
    25441 tttatatcaa gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt
    25501 cccaaatcac aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc
    25561 tacttccgcg aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa
    25621 ggatatcgat ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa
    25681 aggcatagcc tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc
    25741 gtacgacttg cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc
    25801 cttgcacgta atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg
    25861 gtttgccata tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc
    25921 agccactaac gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt
    25981 agtcagcaat atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc
    26041 tcataaaaaa acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac
    26101 tcgaccacat aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta
    26161 ttttgataga gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt
    26221 gtcatagcct cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc
    26281 caactaaacg aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag
    26341 gacagctttt tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg
    26401 gccatgtacc atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac
    26461 atcacatgtt tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg
    26521 tttgaaatca ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag
    26581 atacccgatt ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg
    26641 gagcataggg tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt
    26701 ctcaaattcc aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt
    26761 tgtataatat tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg
    26821 gcagaaacgg tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata
    26881 ttcatccccc catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc
    26941 ttttgcttct gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa
    27001 tccaatgtag cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc
    27061 ggtacgtgaa agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg
    27121 aatggcttct ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg
    27181 gtcagacagt aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg
    27241 caaattcaat gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc
    27301 gtctttccac ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa
    27361 ttcaacgatt ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa
    27421 tgctagttgt ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa
    27481 agtgaagatc agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg
    27541 aatctgtaaa attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc
    27601 taatgaagtg aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa
    27661 actttgcata gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg
    27721 ttctggcgaa tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc
    27781 aaaaggagca agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc
    27841 tacattggca ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc
    27901 gacttcggca ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca
    27961 gcaattcgtc atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt
    28021 ttgatgcgca tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat
    28081 cccggctgtc agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt
    28141 cacgacagtt tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt
    28201 actgcgagca aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc
    28261 gtttttttct agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc
    28321 agcaaaccgg cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata
    28381 agtaagtggt gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat
    28441 cttttgttca tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt
    28501 ctctggctgc tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc
    28561 ctgctaaatt gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg
    28621 ctatatatag tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt
    28681 ttattgtgaa aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt
    28741 tgctgaataa aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca
    28801 taagagcaaa ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag
    28861 aggaggagaa gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag
    28921 caaactttct cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt
    28981 tgttgggatt atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac
    29041 gatcaatttg cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa
    29101 aaacagtgag ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa
    29161 tttgatcgat accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat
    29221 gactaaagag gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga
    29281 actgctcaaa cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc
    29341 tagagcactt gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga
    29401 ctcgaagtcg gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc
    29461 aacgatactg ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt
    29521 tattaaagat ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt
    29581 tgagaaagta atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata
    29641 aactagccgt taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt
    29701 tgactttatc cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag
    29761 tcagaagagc aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca
    29821 ttactgttgt tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc
    29881 aaaaggaaat aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat
    29941 atgtgttaga aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta
    30001 ttattttttc caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta
    30061 gtccattttt tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc
    30121 ttttagcagt atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt
    30181 ttggagaaaa agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac
    30241 tcgcagtcat cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg
    30301 aaatcacttc atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt
    30361 ttttaatttt tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt
    30421 tatttttgat catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa
    30481 tgggtaacac gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca
    30541 ttctcggaat ttctttaatg acaattggcg gcacggctag tatctctact attttatcct
    30601 accaggagaa agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa
    30661 tcaagccgat tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata
    30721 aagtcattgg gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc
    30781 tgatcacgga aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt
    30841 ctttgaaatc acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata
    30901 tttcattata tggatctagt attggactgc cagataatca agaattgaag attcaacaaa
    30961 tgcttccaag tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag
    31021 atgataccgt ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat
    31081 caggtggaaa taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc
    31141 tcaatgctgt ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca
    31201 caaagaaact tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt
    31261 tgaatttctc ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata
    31321 tttttgtcac actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag
    31381 tccggcaatt agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg
    31441 ggattccaca aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc
    31501 cacccatgat attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata
    31561 taaaaactgc cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc
    31621 ttttgcttta tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg
    31681 aataagacaa gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt
    31741 tgtgacacga tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat
    31801 tataaagaaa aaag
//
LOCUS       DACDBG010000025        31177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDBG010000025 DACDBG010000000
VERSION     DACDBG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.025"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(326..1909)
                     /locus_tag="IVZ90_001166"
     CDS             complement(326..1909)
                     /locus_tag="IVZ90_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8262231.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2487..2630)
                     /locus_tag="IVZ90_001167"
     CDS             complement(2487..2630)
                     /locus_tag="IVZ90_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8262232.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2852..3331)
                     /locus_tag="IVZ90_001168"
     CDS             complement(2852..3331)
                     /locus_tag="IVZ90_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8262233.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3404..3688)
                     /locus_tag="IVZ90_001169"
     CDS             complement(3404..3688)
                     /locus_tag="IVZ90_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262234.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3766..3960)
                     /gene="ssrS"
                     /locus_tag="IVZ90_001170"
     ncRNA           complement(3766..3960)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVZ90_001170"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4032..5318)
                     /locus_tag="IVZ90_001171"
     CDS             complement(4032..5318)
                     /locus_tag="IVZ90_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8262235.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5456..6127
                     /locus_tag="IVZ90_001172"
     CDS             5456..6127
                     /locus_tag="IVZ90_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8262236.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6093..6581
                     /locus_tag="IVZ90_001173"
     CDS             6093..6581
                     /locus_tag="IVZ90_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8262237.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6594..7541
                     /gene="prmA"
                     /locus_tag="IVZ90_001174"
     CDS             6594..7541
                     /gene="prmA"
                     /locus_tag="IVZ90_001174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8262238.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7542..8306
                     /locus_tag="IVZ90_001175"
     CDS             7542..8306
                     /locus_tag="IVZ90_001175"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8262239.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8539..10752
                     /locus_tag="IVZ90_001176"
     CDS             8539..10752
                     /locus_tag="IVZ90_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8262240.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10763..11209
                     /locus_tag="IVZ90_001177"
     CDS             10763..11209
                     /locus_tag="IVZ90_001177"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8262241.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11380..12639
                     /locus_tag="IVZ90_001178"
     CDS             11380..12639
                     /locus_tag="IVZ90_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8262242.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12728..13675)
                     /locus_tag="IVZ90_001179"
     CDS             complement(12728..13675)
                     /locus_tag="IVZ90_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8262243.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14069..15361
                     /locus_tag="IVZ90_001180"
     CDS             14069..15361
                     /locus_tag="IVZ90_001180"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8262244.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15376..17145
                     /gene="aspS"
                     /locus_tag="IVZ90_001181"
     CDS             15376..17145
                     /gene="aspS"
                     /locus_tag="IVZ90_001181"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8262245.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17254..18129
                     /locus_tag="IVZ90_001182"
     CDS             17254..18129
                     /locus_tag="IVZ90_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8262246.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18395..20278
                     /locus_tag="IVZ90_001183"
     CDS             18395..20278
                     /locus_tag="IVZ90_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8262247.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20353..20637)
                     /locus_tag="IVZ90_001184"
     CDS             complement(20353..20637)
                     /locus_tag="IVZ90_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8262248.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20627..21361)
                     /locus_tag="IVZ90_001185"
     CDS             complement(20627..21361)
                     /locus_tag="IVZ90_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8262249.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21490..22113
                     /locus_tag="IVZ90_001186"
     CDS             21490..22113
                     /locus_tag="IVZ90_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8262250.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22305..23426
                     /locus_tag="IVZ90_001187"
     CDS             22305..23426
                     /locus_tag="IVZ90_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8262251.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVVDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23433..26561
                     /locus_tag="IVZ90_001188"
     CDS             23433..26561
                     /locus_tag="IVZ90_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8262252.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26580..29774
                     /locus_tag="IVZ90_001189"
     CDS             26580..29774
                     /locus_tag="IVZ90_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8262253.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29920..30627)
                     /locus_tag="IVZ90_001190"
     CDS             complement(29920..30627)
                     /locus_tag="IVZ90_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8262254.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10198 a         5631 c         6582 g         8766 t
ORIGIN      
        1 ttttttacag gatcttctta gggaattttg cttggaaaag tggtcaaaag ggaaaagact
       61 ggcattttat ttattgattt tcaggaaaat cccaatattt tattttcatt catctttata
      121 agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg ggctgtcgac
      181 tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt gatcgaataa
      241 taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc
      301 aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc tggcggctga
      361 tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat
      421 ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt tcttctcgag
      481 catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt ttttctaaaa
      541 agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg atccgtttac
      601 ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact tcttcgtttc
      661 cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac tgcagctgct
      721 gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg taaggctttt
      781 cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg ccgatgatgg
      841 ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact tgatcagcgt
      901 actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca ccattagaca
      961 agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc
     1021 ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca tgtgcccact
     1081 gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc agttcgtaat
     1141 tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg tagtttccgg
     1201 gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact tgatcgagaa
     1261 attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa tagttttcca
     1321 accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg
     1381 catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg ctaagttctg
     1441 ttaaccgtgc ttcctgacag tgtggaatcc ctaagccttg caagatcgct gcaatccggt
     1501 cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg taacgggcca
     1561 gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga ctcatttgct
     1621 gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg
     1681 aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga ctgataacac
     1741 cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt
     1801 tgatatcgac taaggcaatt ttttcaccag cttcaatttg acaggataaa tcctcaaata
     1861 atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg atctctctcc
     1921 ttgagaccac tcaaaaaggc catggttatc cctggcctgt gttatttaaa ctcagattat
     1981 catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg ttttggacaa
     2041 acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg
     2101 ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt ccctccttcg
     2161 tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc gctgacattt
     2221 acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga ctgagggtaa
     2281 tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg aaatcatatg
     2341 gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta ataccgttgc
     2401 attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg
     2461 ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt
     2521 gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa atcggtatcc
     2581 gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac attgacgaac
     2641 caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta atcgccctgg
     2701 tgttcacttt ttttatttta cttgaactca ttgactgttt tccctttatt ttaaacatta
     2761 taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt gtgcaaaaaa
     2821 acctgccgat agttggcagg tcgtctacgt gttaagcttt ggtgtggtct ttagctggca
     2881 attggttttc taaatcagtt cggacaacaa gcacatcgca agctgcgttt ctaataacat
     2941 attcagaaac agaaccaata aataaacgtt ctacagcatt caatcctgtg gctcctaaca
     3001 taatcaaatc tacttgatta tcttctggga tttctctggc aataatttgt tttggtgagc
     3061 catattctac gacagaggtt acattattca agccagcttt cttcgcattg ctttcataat
     3121 ctgccaatgt ttgctttgcc atttcagtag cttgttctgc aagcattcca tcaaaggatg
     3181 aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa tagaagttct ccgttatttc
     3241 ggatagctac attgaccgcc tttttaaaag ctaattcagc ttcatcagaa ccatctacgg
     3301 caaccatgat ttttttgtat tgttgtaaca taagcattca ctcctcaagt caatcgtatt
     3361 gtactctttt attgtagaac actttaggga aaaagagaat tatttactgc tttggttcag
     3421 caattttttt tgcaaatcgg atactgaagg tggcagaaac aataattacg atcagtaaaa
     3481 agaccaaggc aataaagcgt tgattgaaaa aagctgttaa aatacccatt acgcctaaaa
     3541 cagcatatgc agcaccgtaa aattgtagga atccttgatt tttatcattt ttgttgatca
     3601 atacaaaaaa gatatcctgt ttattaaata aataatagct aacgaataat aatactaaag
     3661 tggcgataat cattaaaatc ctaatcatga acaaatcctc caaaataagc aacaaataga
     3721 aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat ctccgttaat
     3781 gccgttgttt gtcctgtagt attgatcccg cgaattcaag caggtgggtc ccatggtcgt
     3841 agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac gggtcccaag
     3901 atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc acagatttcc
     3961 cacctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca gaaacagaac
     4021 cgttttcttg tctagttttt tggaggatgc tttttccact cttttatacg gtaatactgt
     4081 tgacccagag cctgttcata tttccctgta tcttttggct gataatactg agcattcttc
     4141 agtttatctg gtaaatattg ctgattgacc caagcctgat caaaatgatg aggatactga
     4201 taacccacgc cacggttgag tgatttcgca cctttgtaat ggctatctct caaatgatca
     4261 ggaacatctc cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa tgccatgtag
     4321 gcagaattgg attttggcga caagcaaaga tcgacgacga cgtctgctag tgggatacgt
     4381 gcctcaggca atcccaattt ttcagcagct aatacagcgt tcactgtacg agccgcagca
     4441 gcaggattac ctaaacctat atcttcataa ccgataacca tcagacgccg gcaaatactt
     4501 gccagatcac ctgcctcaac taaacgggcg agataatgaa gtgcagcatc cacatcactt
     4561 ccacgaatcg atttttgaaa agccgaaata acgtcataat gagcatctcc atttttgtca
     4621 tgggtaagag cttttctttg gacacattct tcaatgatcg acagtgtaag atgaatcctt
     4681 ccttctttat ctgaaggagt cgatagtgta gccagttcca gcccattcaa tgcactccgc
     4741 aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa ctggattgct
     4801 tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt cgactgccaa ctggatgtct
     4861 tgttctgtta atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc tggattgatg
     4921 gtaatataag gattctcagt agtcgctccg atcaagatga ttcggccact ttctaaatga
     4981 ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa taagaggatc
     5041 actgtaccac tcatttttgc ttcttccgct acaacttgta gatccttttt tgaatctgta
     5101 gcggcgttta gcatgcgaaa tgcataatta gtagagccgg cgatcgcact tgcgatactc
     5161 gtttttccag taccaggagg tccatataga atcatggaag agagcatgcg agcatcgacc
     5221 atgcggcgta tgatttttcc ttccccgact agatgctgct gaccaacgac ttcatcgatc
     5281 gttcgaggtc tcatgcgata agccaatggc tgttgcatag gattttcctc cttcttgttt
     5341 ttacgtccct taattatatc atatttagca agatgcctag aaagaacagc cttctgttgt
     5401 tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga gaaaaatgac
     5461 ggataaacaa aagatagatg aatttttttg gaataaaacg acgccagaag tggcacggga
     5521 tttattgggg atgtatttgg agcatgacac accagaagga agattagctg gatatattgt
     5581 agacgcggaa gcatacctag gtccagaaga cgaggccgcc catagctatg gtttaagaag
     5641 aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt acacgatgca
     5701 tactcatcgg attttgaaca tcattactca gccagaaggg attcctcaag gagtaatgat
     5761 acgtgcaatc gaacccgccg caatgattga tcaaatgagt aaaaatcgtg gcggaaaaac
     5821 tggtccagat atcagcaacg gtccgggaaa attagtggaa gcattagcaa ttcctcaaga
     5881 actttatggt cagtctatag cagatagttc gttacgctta gtttttgaga aaaaaaagac
     5941 accaaaaaag atcaatgctt tgcctagaat cggtatccca aacaaaggtg tatggacaga
     6001 aaagccgttg cgttttgttg tgtcgggaaa tccttatatc tctttacaaa gaaaaaacca
     6061 aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta ttaacatatt
     6121 tagatgaaat aattgaaaaa caagtgacag actatgatgt agctttagat tgggatacaa
     6181 aaaatcatac gatcgagatc gttgttcgct tgtttgcaga aaataaagca caatttgaga
     6241 ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa gacggcgtac
     6301 ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa ttatctagca gtaatccctt
     6361 atgaaggaaa aaaagggttg gcaaaatcag tagctgattc tttagtggaa tatctaaatg
     6421 aagtattagc tcaagggcaa agcgatttgc ttgatttttt ggatgaagac gatgacgaag
     6481 ctgtattcga gcttcactgg gataatcaac aactagcaga attagtagaa gtaaaacagc
     6541 aaaatgaagc agatacttat ttaccgtacc caagttatta aggagtgaca gatatgaaat
     6601 ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga agcagttgct aatattttga
     6661 tggaggcagg ggcgagtggt gtcgcgattg aagattcttt agatgttgaa cattttcagt
     6721 cggatcaata tggtgagatt ttagataaaa atgattttac atcgctgaaa gaaggcgcac
     6781 tagtcatggc ttattttccg gaaacgactt tcttaccgga aatcttgcct tttatcaaag
     6841 atcgtatcac acaattacct gagtttggtc tagctattgg aaaaaataaa gtaacagtta
     6901 gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa atactatcat ccggtgcgta
     6961 ttacgcgaat gctgacggtt gcgccaagct gggaaaacta ccagacagaa gatcctctag
     7021 aaaaaattat cacactagat cctggtatgg catttggcac aggcactcat cccacgactt
     7081 ccttgacatt gacagcacta gaaacagtct tgcgtggtgg tgaaacattg ctagatgtgg
     7141 gaactgggtc gggcgtgcta agtattgcag caaaatatct tggtgcaaaa gatgtctatg
     7201 cctttgattt agacgaagta gccgttcgtt cggcgaaaga aaatatggat atgaatgaaa
     7261 tagccaaaga tgtccatgta tcagctaacg atcttttgac aggaatcgag attgaaagtg
     7321 atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatccca gatgcttggc
     7381 gtttactgaa gcaaacaggc acgctgatcg tttcaggaat cattgaagag aagaagcaaa
     7441 tggttctgga tgccatgtta gaacaagggt ttatcgtgga tcaaattttc cagcaaaaag
     7501 actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcgg tattttttaa
     7561 aagaatcata tgaatcaaaa gactattttg ttttagaagg ggaaaactat caccacattg
     7621 tacgggttat gaggatggaa cccgaggatg aagttttttt ggcattcaat gatcaaatag
     7681 ccattgttgc agaaatcacg gagattactg aagaggctgt ttatctgaaa gaaattaaaa
     7741 aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc tatcctaaag
     7801 gggaaaagct ggattggatc gttcagaaat ccactgaatt aggagcgcaa gcttttcttg
     7861 gtttcccggc atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg aaacgacaac
     7921 aacggcttga aaagattgct caagaggcag cggaacaatc ccatcggcag atcgtgccat
     7981 cagtagagtt attggaaacg aaacaagatt tatttgagcg gctagatgat tatgacgtgg
     8041 tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca gccaaaattc
     8101 ttagtgaaac aaaaccagat agtcgaatct tagctgtctt tggaccagaa ggtggattat
     8161 cccgcgatga agtcgattat tttgtagaac acaaagggat actttgtgga ttaggaccaa
     8221 gaatccttcg tacagaaaca gcacctcttt atttattaag tgctgctagc tatcaatggg
     8281 aattaatgaa tccttctttc agctaacgtt cttcattaat aaaagagtat cgacagaaag
     8341 aattgaaaaa atagtcaaca atttgtataa tagaaacaat tatttataca atggatacga
     8401 aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg tataactgca
     8461 cggattttgg gacaattatc aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag
     8521 aaagggggat cgattcctgt gacaaaagaa gaaattttaa cgggacccgg tgtcataaag
     8581 ctcgttagtc aatacatggg tcccgaacat gtagcatttg ttgagaaagc atgcgaatac
     8641 gcgactgctg cacatgacgg acagtttaga aaatcaggcg aaccctacat tattcatcct
     8701 atccaagtcg caggtatatt agcagattta aaaatggatc cccatacagt ggctacaggc
     8761 ttcttacatg atgttgttga agatacagaa atcactttag aagatctgag agaagaattt
     8821 ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat tagggaaaat aaaatataaa
     8881 tcccacgaag agcagctagc agaaaaccac cgaaagatgc tgctagcaat ggctcaagat
     8941 ttacgagtta tcatggtcaa attagctgat agactacata acatgcgtac gttgaagcac
     9001 ctgcgagaag ataagcaaag aaggatcgct caggaaactt tagaaatcta tgcgcctctt
     9061 gcacatcgtc tagggatcag ccggatcaaa tgggaattag aagatacagc acttcgttat
     9121 ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc agacgaagag agaagaacgt
     9181 gaaaaatacg taagtggtac tgttgaagat attcgaatag cgacagaaga gctggggatt
     9241 tttgcagaaa tctatggacg gccaaaacac atttattcaa tctatcgtaa aatgaaagat
     9301 cagaaaaagc agttcaacga aatttatgac ttgctagcta ttcgagtgat cgtagattca
     9361 atcaaggatt gttatgctgt attaggagca atccacacaa aatggaaacc aatgcccggc
     9421 agatttaagg attatattgc catgccaaaa gctaatatgt accaatcttt gcatactacc
     9481 gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc gaacacagga aatgcatgaa
     9541 atcgctgaat tcggggttgc tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta
     9601 gaaccagatg gtatgacgaa acaattaagc tggttccatg agatactcga acttcaagac
     9661 gaaagctatg atgcttctga atttatggaa ggcgtaaaag gagatatctt tagtgataaa
     9721 gtctacgtct tcacaccaaa aggagacgtt actgagttac caaaaggatc cggaccattg
     9781 gactttgcat acagtatcca tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat
     9841 ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag atatcattga gatcatgact
     9901 tctccaaatt catttggccc aagtcgcgac tggttgaaat tagttgctac tagcaaagca
     9961 agaaataaga tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc
    10021 cacgaatccg tggtcaaatg tattacagat ctaggattta cgcctaaaga tattttgacg
    10081 aagaacaaac tgcaagaagc actcgatcgt tttaattatc aaacagaaga tgatctctat
    10141 gcagctgtag ggtatggaga agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa
    10201 gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat catgaatcag
    10261 ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg
    10321 gtagaaaact tactaattcg tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc
    10381 gttggttata tcactaaagg cagagggata tccattcatc gtcgagattg tccgaatgtt
    10441 cagcctgaca aaccaaatgt agcagaacgt ttgattgaag tcgaatggga agatacatcg
    10501 aatacacgaa aagagtatga tgcagatttg gaaatttacg gctataatcg ttcaggctta
    10561 ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca
    10621 cggacgaata aagataaaat ggcgacgatc catttgacgg ttggtatcca gaatttatcc
    10681 cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg atgtctacag tgtacgccgg
    10741 acgaatggat aggaggacag atatgagagc agttgttcaa cgggtaagta aagctgctgt
    10801 gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc ctacttgttt tattaggcat
    10861 tcatgaaacc gatacgcaaa aagatgtgga ctatctggta aaaaaaattg cacaaatgcg
    10921 tatttttgaa gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat
    10981 tttaagtatc tctcaattca cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt
    11041 tgtggaagcc gcacgaccag aaacagctat tcctttatac gaatcattca atgaaggaat
    11101 cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt cggtttctct
    11161 cgtcaatgat ggacctgtaa cgatcattat tgattctaaa aacaaataaa gtaatctggt
    11221 tttcactagt aaaaactatg aaacacgtcg cttaagttag actgataaac tctaacttaa
    11281 gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa aagttaagct
    11341 taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt accagtagta
    11401 gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat gttggaggag
    11461 ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa agaagctatc
    11521 acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt caaacgaaaa
    11581 ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt acagactttg
    11641 gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac accggaaaga
    11701 atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga agcacagcat
    11761 ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat ttgcgagtgg
    11821 gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc agaatatcaa
    11881 atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg tgtcgagaat
    11941 cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca aaatcctgtg
    12001 gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt agatggtcag
    12061 cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc aacggttgct
    12121 ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga tttgtatcag
    12181 caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat gagacgctat
    12241 tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt agatggttat
    12301 cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca tgatgaacga
    12361 acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc aaaagatatt
    12421 tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg acaaatgcat
    12481 cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga tcagctagta
    12541 aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa tcagtttccg
    12601 acttatccga atagccgagc gctactttac aagaattaga ttagcataaa cttatcgaaa
    12661 aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa ttgataaggt
    12721 caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca ttggataaat
    12781 gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct ccaggtataa
    12841 ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca ggagtggctt
    12901 ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct ctgaaacgtt
    12961 ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt ttttcctcac
    13021 caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga acaaaactga
    13081 ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg atcaaactgt
    13141 atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat tgttctaacg
    13201 tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg tgcaatgtag
    13261 caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa atgtcgatgt
    13321 aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac tcaggagaat
    13381 tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg acgacacgtg
    13441 agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa cggccgtttc
    13501 cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta cgaactactt
    13561 gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg gcatttgctc
    13621 ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct gccatagctg
    13681 attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa aacgtttttg
    13741 tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag gttataagta
    13801 atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag aggatcatcg
    13861 aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg gacgagtgaa
    13921 aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc aaaattaggt
    13981 ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga tttttttatt
    14041 tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag gaacaaatga
    14101 tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac gtttattatt
    14161 cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg aagtgatatc
    14221 ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt tttatgacaa
    14281 aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc gttcttttgt
    14341 tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt atatgggacc
    14401 aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc aaatcggagt
    14461 agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga tggcacttga
    14521 tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat taggtgataa
    14581 agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt ttgaatcaga
    14641 gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat tggacagtaa
    14701 agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt atctaagcga
    14761 gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg ctgttcctta
    14821 tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga tttttgaaat
    14881 catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg gacgttatga
    14941 caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg caatgggtat
    15001 cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat ttaatgagtt
    15061 ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga aagtggtcca
    15121 agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta aagcaaaagc
    15181 gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg gtgaagatga
    15241 gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa aaagttttcc
    15301 attagaagag atatatgaac attttgatga agtctatgat gaaatgactt tattcgagta
    15361 ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc cgaacaacaa
    15421 ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct tgggggagta
    15481 atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa tcctgaaatc
    15541 aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat cgaagtgact
    15601 ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac tggtgaattc
    15661 gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc atttcaaatt
    15721 gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt ggatctgcgt
    15781 cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc gattcgtcat
    15841 tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa atctacacca
    15901 gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt ctatgcgttg
    15961 ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga ccgttattat
    16021 caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc tgaatttacg
    16081 caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta cacagaggga
    16141 ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc atttcctcgt
    16201 atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac acgttttgat
    16261 atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt cttccaaatg
    16321 gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc agatcgctac
    16381 tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc taaggggctt
    16441 gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt catgggagaa
    16501 gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct gatgtttggc
    16561 gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg tttaggaaaa
    16621 gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga ttggccacaa
    16681 tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt cacgatgcca
    16741 aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc agaagcttat
    16801 gatatcgtct tgaacggtta tgaattaggc ggaggttcta tccgtattca tactcgtgag
    16861 cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga agaacaattt
    16921 ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat tgcattagga
    16981 ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt cattgccttt
    17041 cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt ttcgccttta
    17101 caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat aaaaaggtaa
    17161 aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa acactttata
    17221 atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga aaattgggct
    17281 tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat cgcatcagtc
    17341 gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac tggtctagct
    17401 cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt atcaacaact
    17461 ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg gaagaagttt
    17521 acaattttca cttttatcac agtcactttg acctcccttt gtatccatat tttaccggaa
    17581 acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt gatgggacta
    17641 ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat cgtgagtatc
    17701 acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt caatgcattg
    17761 atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag tacgttgtct
    17821 attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa gatgcaggta
    17881 atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg tatgcgtaga
    17941 gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca gacagtattg
    18001 atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa agcttctgat
    18061 ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt ctatgaggat
    18121 ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag acgttcgttt
    18181 gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt tttatttgaa
    18241 attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga tgatagaaaa
    18301 aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc gtatattacg
    18361 aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa
    18421 cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg gtgttatctt
    18481 ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga tttttttcgt
    18541 cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa atggaaaaat
    18601 caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac ttgtttttat
    18661 gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa acaatcttcg
    18721 aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca ataacggttt
    18781 atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt ctttagcagt
    18841 agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg tatttgttcg
    18901 taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg ccatcttgat
    18961 gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta ccgacactcg
    19021 tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa
    19081 gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa tctcgctttt
    19141 ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct attacggagg
    19201 aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac accccggagc
    19261 aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac gaagctatgg
    19321 catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga ctgatattgc
    19381 cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca tcagtgaact
    19441 ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta cattccaagt
    19501 gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg ttgttccagg
    19561 cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac
    19621 agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac
    19681 cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg ataagtacga
    19741 gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat ttggtgattc
    19801 agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag tggtagatgg
    19861 agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa aggaacaaga
    19921 tttgttgaaa gacactgttc tgattggact aggaacgaat ggctcattct cggaagctca
    19981 gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca atgtccgagt
    20041 accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata
    20101 cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg actggtttta
    20161 tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact tagtcagcga
    20221 agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga cagaatgata
    20281 gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt atatttaccg
    20341 tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct gtaaataacg
    20401 ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg cttcgcttct
    20461 tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa gacgagtata
    20521 tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc
    20581 accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat ttgccataga
    20641 gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc aatggtggag
    20701 cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa atatttgcct
    20761 cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga tagtcttcca
    20821 tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag cgtccatttg
    20881 tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga atctcatgac
    20941 gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct ttgaaataag
    21001 gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag ctgtctttca
    21061 gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata ctacgtcttg
    21121 ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg gttcgtttac
    21181 ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc ccttttttag
    21241 gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac actttggaac
    21301 tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct tcgtgaagca
    21361 tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt attaaaaaga
    21421 accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa gaagggaaag
    21481 gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt agccttagtt
    21541 aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa ttacatacta
    21601 gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc agcacccaag
    21661 gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg atttatatta
    21721 actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt cattaaaaca
    21781 cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag tgggacaaga
    21841 catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa agcgaaaatc
    21901 gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg tcagagagtg
    21961 acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga taaagaaggt
    22021 atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa agaagtgaac
    22081 cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg tgggcggtct
    22141 tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg agagacgtca
    22201 agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca tgtaatggat
    22261 caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt tttttacata
    22321 cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa gaccagcgtc
    22381 atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca atcgtgattg
    22441 ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta tacaatgaat
    22501 tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca ggaaatcacg
    22561 atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac tattttttgc
    22621 gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa tttttctttc
    22681 ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat atccgaacga
    22741 tcaagcaagc ggtagccaag gtggtagata aaatgcagga gctatttgat tcagaaaaaa
    22801 aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga
    22861 aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg tttgattatg
    22921 tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct cgctatagcg
    22981 gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg
    23041 acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat gatattgaag
    23101 aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg
    23161 agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg atgaaccagc
    23221 ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga gcagaacaaa
    23281 caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca gaagcatttt
    23341 ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc ggagaaacgt
    23401 tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa
    23461 attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa gcaccgcttt
    23521 ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg atcaccttcg
    23581 cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt tctttgtttg
    23641 ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag atgagctatg
    23701 aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga acaaaaaaac
    23761 aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata aaacaaatca
    23821 caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat gcgaaacagt
    23881 tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt tcatcaagca
    23941 gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa cagttcaatg
    24001 agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta gagcaagagc
    24061 tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca gcagggtttg
    24121 aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa atcacggaag
    24181 aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat tcctactatc
    24241 gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac aagcaaaagg
    24301 aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg tattatgaat
    24361 ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt gaaaaaatag
    24421 aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg cagaattatg
    24481 aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg
    24541 ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa
    24601 aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat gccgagaagc
    24661 tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa aatttactgg
    24721 atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat ctagatgaaa
    24781 agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca cagataagag
    24841 aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg aaagaacaga
    24901 cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct cgtcttcagc
    24961 tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac cccatacaac
    25021 aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa caactggcag
    25081 aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa gcacatgaaa
    25141 ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg aatatacaat
    25201 tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct gagataacag
    25261 aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga cgaaaaagca
    25321 agatggccaa tgaaagaata gagctggaag aaataagaca acaagcagaa aagctcgaac
    25381 aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc cagcaactgc
    25441 aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac tgtttagaag
    25501 aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta gctgttgagc
    25561 agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag cttgatcttc
    25621 aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa agaatagcta
    25681 aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca tttgcacaac
    25741 agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa gagcaattag
    25801 tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca ttcccagata
    25861 cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata aagcaagaaa
    25921 agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt
    25981 cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag cagctttcgc
    26041 aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg cttcaggttt
    26101 atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt
    26161 atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga ttagaaatca
    26221 atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga
    26281 gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag acacaagctg
    26341 gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta gatgaagaag
    26401 ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga atgatcggca
    26461 tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga attgatacag
    26521 aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga ttggtaacta
    26581 tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg caagaaggcc
    26641 gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca
    26701 gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac tgccaatgtc
    26761 cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt tatttacggt
    26821 cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg gtaaaagcaa
    26881 caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc
    26941 aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact aatcagtatc
    27001 acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca tcaccgaaaa
    27061 aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa aaagaaactt
    27121 acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc
    27181 aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta ggtacgaacg
    27241 gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta gaacaagccc
    27301 gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata aatgaacaaa
    27361 acgatcaagg aatacaattt tctgatcaaa acaaaccttt atcattcaaa gtggaaaaat
    27421 ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt atgcattatc
    27481 agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa acggtttatc
    27541 aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc aagaaaaaag
    27601 aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc gacgtagcta
    27661 ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt ttctttttcc
    27721 gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggtgaa gtcatctatt
    27781 cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta cgtgacacag
    27841 tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa aatgaaactg
    27901 gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc acagaactat
    27961 ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag ttgtatttag
    28021 atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg ctcgatattc
    28081 gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa agcttgctga
    28141 gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat tcagaagaat
    28201 ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa
    28261 cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa aaaagcaatg
    28321 ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca gaaagatacc
    28381 aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc
    28441 gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat gaaatgggac
    28501 ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa cggaaaagct
    28561 tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca gaggtgagaa
    28621 aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat
    28681 tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga caggatctag
    28741 ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa atggtcaaaa
    28801 atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag cggtttgcac
    28861 tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt gcaacgatta
    28921 tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc
    28981 aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg
    29041 aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact gtttatttag
    29101 cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca ttcaagaaaa
    29161 accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc
    29221 tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa gatctactgg
    29281 tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca gggatgctga
    29341 ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta agaggcagta
    29401 cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa aaagatgttt
    29461 ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca gatacggtca
    29521 tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga cgtgcacatc
    29581 ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga acgattgagg
    29641 aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta
    29701 atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta agtgtaggag
    29761 aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa
    29821 gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca ttttatccca
    29881 gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga ttgtcttcat
    29941 tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag tctgttcaat
    30001 tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg cacctagaac
    30061 atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat gaaattcttt
    30121 ctcagccagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt gagaaatgag
    30181 catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg
    30241 gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca agaaatcgag
    30301 ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt aaattttttg
    30361 aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta gtgattggct
    30421 gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt ttgggaagtt
    30481 ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat ctgaaacaag
    30541 cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc
    30601 aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt cattgatttt
    30661 ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga taaataaagg
    30721 tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt cagttttttt
    30781 cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat tatggtagaa
    30841 gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta tttttttaat
    30901 acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt tgtttatagt
    30961 aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacaga gaaaataagc
    31021 gttataataa agaacagtat tatttaaaat gacagaaaac attactatta aagaaataaa
    31081 aaacataaaa aatcttaaga aatcggtttc atttagcttt tgagataaaa taatagtaca
    31141 gttttatacg ctgtttcata tgtttagaaa atttgaa
//
LOCUS       DACDBG010000026        30329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDBG010000026 DACDBG010000000
VERSION     DACDBG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.026"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IVZ90_001191"
     CDS             173..1309
                     /locus_tag="IVZ90_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8262255.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IVZ90_001192"
     CDS             1446..1790
                     /locus_tag="IVZ90_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8262256.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IVZ90_001193"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IVZ90_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8262257.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IVZ90_001194"
     CDS             complement(3485..4084)
                     /locus_tag="IVZ90_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8262258.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IVZ90_001195"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IVZ90_001195"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8262259.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IVZ90_001196"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IVZ90_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8262260.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IVZ90_001197"
     CDS             7290..8408
                     /locus_tag="IVZ90_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8262261.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IVZ90_001198"
     CDS             complement(8468..9760)
                     /locus_tag="IVZ90_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8262262.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IVZ90_001199"
     CDS             9910..10470
                     /locus_tag="IVZ90_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8262263.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IVZ90_001200"
     CDS             10599..10976
                     /locus_tag="IVZ90_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8262264.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IVZ90_001201"
     CDS             10909..12981
                     /locus_tag="IVZ90_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8262265.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="IVZ90_001202"
     CDS             complement(13192..14139)
                     /locus_tag="IVZ90_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8262266.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="IVZ90_001203"
     CDS             14385..14849
                     /locus_tag="IVZ90_001203"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8262267.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="IVZ90_001204"
     CDS             complement(14911..15954)
                     /locus_tag="IVZ90_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8262268.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="IVZ90_001205"
     CDS             complement(16331..17296)
                     /locus_tag="IVZ90_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8262269.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="IVZ90_001206"
     CDS             17530..18375
                     /locus_tag="IVZ90_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8262270.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="IVZ90_001207"
     CDS             18566..19753
                     /locus_tag="IVZ90_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8262271.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="IVZ90_001208"
     CDS             19759..20115
                     /locus_tag="IVZ90_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8262272.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="IVZ90_001209"
     CDS             20117..20455
                     /locus_tag="IVZ90_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8262273.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20850..21884
                     /locus_tag="IVZ90_001210"
     CDS             20850..21884
                     /locus_tag="IVZ90_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8262274.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="IVZ90_001211"
     CDS             21877..22563
                     /locus_tag="IVZ90_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262275.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="IVZ90_001212"
     CDS             22587..23435
                     /locus_tag="IVZ90_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8262276.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="IVZ90_001213"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="IVZ90_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8262277.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="IVZ90_001214"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="IVZ90_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8262278.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="IVZ90_001215"
     CDS             25701..26936
                     /locus_tag="IVZ90_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8262279.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="IVZ90_001216"
     CDS             26923..27393
                     /locus_tag="IVZ90_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8262280.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="IVZ90_001217"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="IVZ90_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8262281.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="IVZ90_001218"
     CDS             complement(28884..30026)
                     /locus_tag="IVZ90_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8262282.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..>30329)
                     /locus_tag="IVZ90_001219"
     CDS             complement(30065..>30329)
                     /locus_tag="IVZ90_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8262283.1"
                     /translation="FNILKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQ
                     VEDMNYPEEAVNEIIDNINKLKQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         9866 a         5584 c         6439 g         8440 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgatcac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccatgaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttttagcttg tttatattat caataatttc gtttacagct tcttcgggat
    30181 aattcatgtc ttccacttga ctatgtatcc cttcatctat catttcttct agttgttcct
    30241 ctatactgct agtgagcaga agaatgttat tgtcaaattc ttctataata gcattgatga
    30301 ataattcttc tatttttaag atattgaaa
//
LOCUS       DACDBG010000027        29988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDBG010000027 DACDBG010000000
VERSION     DACDBG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.027"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(220..1947)
                     /locus_tag="IVZ90_001220"
     CDS             complement(220..1947)
                     /locus_tag="IVZ90_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8262284.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1940..3133)
                     /locus_tag="IVZ90_001221"
     CDS             complement(1940..3133)
                     /locus_tag="IVZ90_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8262285.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3320..3964)
                     /locus_tag="IVZ90_001222"
     CDS             complement(3320..3964)
                     /locus_tag="IVZ90_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8262286.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4058..4192)
                     /locus_tag="IVZ90_001223"
     CDS             complement(4058..4192)
                     /locus_tag="IVZ90_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8262287.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4194..6326)
                     /gene="feoB"
                     /locus_tag="IVZ90_001224"
     CDS             complement(4194..6326)
                     /gene="feoB"
                     /locus_tag="IVZ90_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8262288.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6323..6796)
                     /locus_tag="IVZ90_001225"
     CDS             complement(6323..6796)
                     /locus_tag="IVZ90_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8262289.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7197..7421
                     /gene="nrdH"
                     /locus_tag="IVZ90_001226"
     CDS             7197..7421
                     /gene="nrdH"
                     /locus_tag="IVZ90_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8262290.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7580..9739
                     /gene="nrdE"
                     /locus_tag="IVZ90_001227"
     CDS             7580..9739
                     /gene="nrdE"
                     /locus_tag="IVZ90_001227"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8262291.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9789..10754
                     /gene="nrdF"
                     /locus_tag="IVZ90_001228"
     CDS             9789..10754
                     /gene="nrdF"
                     /locus_tag="IVZ90_001228"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8262292.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10843..11982)
                     /locus_tag="IVZ90_001229"
     CDS             complement(10843..11982)
                     /locus_tag="IVZ90_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8262293.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12291..13004)
                     /locus_tag="IVZ90_001230"
     CDS             complement(12291..13004)
                     /locus_tag="IVZ90_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8262294.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13258..13959)
                     /gene="nagB"
                     /locus_tag="IVZ90_001231"
     CDS             complement(13258..13959)
                     /gene="nagB"
                     /locus_tag="IVZ90_001231"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8262295.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14193..15725
                     /locus_tag="IVZ90_001232"
     CDS             14193..15725
                     /locus_tag="IVZ90_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8262296.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15916..17118)
                     /locus_tag="IVZ90_001233"
     CDS             complement(15916..17118)
                     /locus_tag="IVZ90_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262297.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17200..17889)
                     /locus_tag="IVZ90_001234"
     CDS             complement(17200..17889)
                     /locus_tag="IVZ90_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8262298.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(17995..18363)
                     /gene="rplL"
                     /locus_tag="IVZ90_001235"
     CDS             complement(17995..18363)
                     /gene="rplL"
                     /locus_tag="IVZ90_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8262299.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18416..18919)
                     /locus_tag="IVZ90_001236"
     CDS             complement(18416..18919)
                     /locus_tag="IVZ90_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8262300.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19329..20018)
                     /gene="rplA"
                     /locus_tag="IVZ90_001237"
     CDS             complement(19329..20018)
                     /gene="rplA"
                     /locus_tag="IVZ90_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8262301.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20122..20544)
                     /gene="rplK"
                     /locus_tag="IVZ90_001238"
     CDS             complement(20122..20544)
                     /gene="rplK"
                     /locus_tag="IVZ90_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8262302.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20887..21555
                     /gene="sdaAB"
                     /locus_tag="IVZ90_001239"
     CDS             20887..21555
                     /gene="sdaAB"
                     /locus_tag="IVZ90_001239"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8262303.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21568..22440
                     /gene="sdaAA"
                     /locus_tag="IVZ90_001240"
     CDS             21568..22440
                     /gene="sdaAA"
                     /locus_tag="IVZ90_001240"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8262304.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22531..23550)
                     /locus_tag="IVZ90_001241"
     CDS             complement(22531..23550)
                     /locus_tag="IVZ90_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8262305.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23723..24610)
                     /locus_tag="IVZ90_001242"
     CDS             complement(23723..24610)
                     /locus_tag="IVZ90_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8262306.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24687..25232)
                     /gene="nusG"
                     /locus_tag="IVZ90_001243"
     CDS             complement(24687..25232)
                     /gene="nusG"
                     /locus_tag="IVZ90_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8262307.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25333..25503)
                     /gene="secE"
                     /locus_tag="IVZ90_001244"
     CDS             complement(25333..25503)
                     /gene="secE"
                     /locus_tag="IVZ90_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8262308.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25527..25679)
                     /gene="rpmG"
                     /locus_tag="IVZ90_001245"
     CDS             complement(25527..25679)
                     /gene="rpmG"
                     /locus_tag="IVZ90_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8262309.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25841..26485
                     /locus_tag="IVZ90_001246"
     CDS             25841..26485
                     /locus_tag="IVZ90_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8262310.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26541..27044)
                     /locus_tag="IVZ90_001247"
     CDS             complement(26541..27044)
                     /locus_tag="IVZ90_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8262311.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27057..27103)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27641..28606
                     /locus_tag="IVZ90_001248"
     CDS             27641..28606
                     /locus_tag="IVZ90_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8262312.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28705..29877)
                     /locus_tag="IVZ90_001249"
     CDS             complement(28705..29877)
                     /locus_tag="IVZ90_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262313.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29864..>29988)
                     /locus_tag="IVZ90_001250"
     CDS             complement(29864..>29988)
                     /locus_tag="IVZ90_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262314.1"
                     /translation="LYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9133 a         6174 c         5353 g         9328 t
ORIGIN      
        1 ataataaact ttaatcacat tagtctttca tacctacgat cgatctcttt gactcgcacc
       61 cataaattca taatagtttg tcggcgtctt tggacgtttc gaagatgagc tacgcattct
      121 ctttttttat agacaaaaaa gatagagacg aacatggagc ttccaaattt ggatatttga
      181 tccaattttg gaagctccat ttatttcgtg tctgtctttt tattttttcc agctttcgac
      241 taattcttgg ttctctttga tccagtcttt tgctgcttgc tgtggatctt ttccattgtt
      301 gatttccaac ataacttttt ccatatcttt ttctgaccaa tggaaattgt ctaatacttt
      361 gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc atcgtatgga tctgctcttc
      421 gcttcccatt ccgttttcag gatcttctaa gtatttcaga tcatatttgg caaacatcca
      481 atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt ttgatcgctt gacctagtgc
      541 aacggtcatt gctcctgaag aagatgtctc tactttccag tctttcaagt tgtcatattt
      601 ttggatcgtg ttttcagcag ccgtaactac accagctcct ggctcgattc ctatgatttt
      661 cttacctgcc tgatcggtta actcatcgat cgaatttaca tccatgtagg ctggcactgc
      721 taaaccaact tttgctcctg tcaaattggc acctaggtct tcgacttgat ctttatattg
      781 tgcatattgt gaaccatgag ttttaggtag ccaagctgaa accatggcat ctgactcgcc
      841 atttgaaaca gatttccaca tgattgaatt atctaatggc gtcatcgtta catcatagcc
      901 catttgtttc aatacttcac cgactacatt tgttgaagca acttctgtat cccattcaac
      961 ataagataag ttgatacgtt ttgtttcttt ggcagatgaa aaactcccta taccaatcgc
     1021 acctaagatc actactacta cagcaccaat gatcagtcct tgacgttttt tacttttctg
     1081 tgttgttgct cctgcagctt ttttattgtt tggctggttc aaatgctgtg taaaacggtc
     1141 aatgatgatt gctaagataa ctaatgcaac cccgttgaca aatccattcc cgacttgtgc
     1201 tctttgcaat gctgataata cgccgcgtcc taagccgggt gcaccgatca tagaggcaat
     1261 aacgaccatt gaaagtgaca gcattgttgt ttgattgatc cctgccatga tcgtactttt
     1321 agctaaaggc aattctagtt tgaatagttt ttgacgactt gtgctcccga atgaatcaga
     1381 agcttctact aattctttag gaacttgtcg aatccctaaa ttcgtaaaac gaacggttgg
     1441 tggcagagca aagatgactg aggcaaatac ccctggaacc atcccgatac cgaaaaatgc
     1501 aactgctgga atcaaataaa cgaatcctgg cattgtctgc ataaagtcaa ggatcggttt
     1561 gataatactt tgggctttac tgctttttgc catcaagatc cctaatggta ccccaatgat
     1621 gatcgagatc acactagata aaagaacaag tgtgacagta ttcatcaaat cattccatag
     1681 accttggtta taaataaacc atagaccgat taatgtgaac aacgtaagtc ctggacgttt
     1741 cttagaaata aagaaagctg ctaccgtcaa taacaagata aacaaaggag ctggaatcac
     1801 taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca atcgtttgta aaaatgaaaa
     1861 taggccagaa aaagttgtcg taaaccattc tgtgatattt tctacccagt ttgccactgg
     1921 taattgatag ttcaatagat tattcatgtt cactcacctc gcttgtttct gctaatgctt
     1981 caatcacact acctcggatg acgacacctt ttaattttcc atcctctaca actgcaagtg
     2041 gagtcggtga gtcataaata atattgaaaa tatcacttac caccatgtct ttcccaatca
     2101 ccgtcacatt ttcatctagg aagtcgatca atggacgttt ttgcttccta gcttctaacg
     2161 cttgatctgc agtgatgatc cctttcaggt gtctctttct gtcaacagcc atcagcatgc
     2221 tgacttcttc attacgcatc cttgtcaatg caacgtttgg tccatcagat tcgatattcg
     2281 tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac tttggaacga tcgacttctt
     2341 ccacaaattc acgtacataa tcatttgctg gattggtcaa tatttcttca cctgttccga
     2401 tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc cccaatccgt aaagcttcat
     2461 tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt ggcttgcagg tccagtaatt
     2521 cgtcttgcat ttctcgccgg atcagcggat caagagccga gaacgcttcg tccatcagca
     2581 agatctcagg atcatttgct aaagctctgg ctaggccaac ccgttgttgc atcccaccag
     2641 ataactgact aggatattgg tctttaaatg aaagcaaact agaattttct aatgcttttt
     2701 ctgctttttc ttgtcgttct tctttaggta caccacggac ttctaacccg tattctgtat
     2761 tttccaagat cgttcgatgt ggaaacagac cgaagttttg gaaaaccata ttgattttat
     2821 gacgacggac ttctcgcaat tcttctttgc ttaacttcga tacatcttct ccatcaatat
     2881 agatactgcc cgaagttggc tcaatcagac gattcaatag acggatcaat gtggatttcc
     2941 cactcccgga aagccccatg atcacgaaga tttctccttc ttttacgtca aaattcacgt
     3001 catacacacc aaccgttgca ccggttttct ttaaaatctc cgttttcggc tggtgatcat
     3061 tcatcatatc taaagcggct tgtgtctttt taccaaatat ctttgttaaa tgttttactt
     3121 ctacttttac caaacaaatt ctcctttttt atgtaaattc atttttcttt ctgaaaaaag
     3181 tgaccacact atgttaacat catgtagtca ctttgtcaaa aatgaaagaa atttgcttgt
     3241 ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa ggtttagcca agttaatatt
     3301 tttttaatat ttcacacaat cattctgcaa gctgatgctc cgggtaaaaa aagtttgtca
     3361 tacgctgtag tgtatattca tttccaataa aatgaacgat gtcccctgcc gtaaatttag
     3421 cataggggcc aggtgaaaca agcagttctt cattatgaga aattgcgata actgtggcgc
     3481 cagtcgcctg ccagatattc aactcgctaa ttgaacggtc cagatgcatt gctttttctg
     3541 ataaagataa ttcaaacgga agtaaaggat tcatcttatg tattcgcttc gtttgattga
     3601 ccagctgatc caataattga gaaaaatgat tcagctcatc ttgttgcttc tccacacttg
     3661 ccatgatttc tgcttttgta tcttggatgg attgtacatc ttgatattgc aataggaaat
     3721 cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc atgccgtact tccatgattc
     3781 caagatctac cagcacattg atagccttgc gggtagtctc aggagaagca ttgtacgtac
     3841 tggcaagtgt tgagcgggca tggatcttct cacccacttt atatcggttt tcaacgatac
     3901 gttcggctaa atcaacagca atctgctggt agcgcgggcg gacgattttt ttctttccag
     3961 acattttctt tcctctttct ttttttactt tccctcatct tactatgctc tagcaaaata
     4021 agcaaaaaag gggagaatag cctcttgcaa cgactgatca tcgcttgatc tgttcctttt
     4081 ttactgggca agctgtttga cagtcatcac aattcttacc ggattttaaa cgagtataga
     4141 caattgcgcc tgcacttcca aagattaaca cacttaataa gaatgtcgcc atcctattcc
     4201 tctccttcta atgacaatat agtataaaac tggttcttgg attttggctg acggaacaaa
     4261 acataaacga gtcctatcat cagtaaaata gcaagatacg tccctaacgc tacctgtcca
     4321 ccctccaata agtgtcccaa ctgataaatc acaaaactaa ctaaataagc taaaccgcat
     4381 tgatagccta tggcgatcca agtccatttc ttggcattca tttcccgatg gatagcccca
     4441 attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta acaatgaata agctgctgcg
     4501 ggagtatatg tgtgttgtag cacttgccag atttctctgc cattttcgga tacttccgca
     4561 tgaccaaaca aaataccaaa tgtgccaata atattttctt tagcgaccaa tcctgtaatc
     4621 gctgcgactg ttccttgcca gtttccccaa ccaagcggct gaaaaacaac tgccaggatt
     4681 cgcccgaaaa aggctaagat actttggtct tcagaaacag gttgagcata aaaattataa
     4741 gaagagctga accagatcac gatactagaa acaaagataa tcgttcctgc ttttttaaca
     4801 aaagacttgc tgcggtcata ggtttgtctt aggaccgctg accattgagg aaaatgatat
     4861 gcgggcaatt ccatgataaa aggagctgta tctccagcaa acaaacgtgt ttttttcaag
     4921 ctgattccag agaaaataat tgcagtcata ccaagaaaat aggcagaagg agctacccaa
     4981 gattgatgag gaaaaaaggc ccctgctact aaggcaataa tcggcaactt tgcagaacaa
     5041 ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt gctcgttttc aatcgtgcga
     5101 ctggccatga cgcctggcac tccacatcct gtagcaatca gcataggaat gaaggatttt
     5161 cctgaaagac cgaatttcct gaaaagtcga tccatgacaa aagcgatcct cgccatatat
     5221 ccgcagtctt ccagaaagct caagcataaa aacaatataa tcaattgcgg tagaaatcct
     5281 aacactgctc ccacgcctgc gatgatgccg ttcaagatca gctcctgcat ccatcccgct
     5341 acttgccatt cagctaacaa tctgccaaca tgttctggta accatgtacc aaacaatgtg
     5401 tcattgatcc aatctgtccc cattgttcct actgtttgaa tagccagata atagatcagc
     5461 cacatcacaa atgcgaagat cggcaaggct aaccaacgat tcgttgcaat ctgatcgatt
     5521 ttgtcactca tcgataattg gaaagaatca tttgacacaa cacagagtgt acatagttgg
     5581 gtaataaatt cataacgttc attgaccaga atcgtctcac tatcgtcgtc caatagtttc
     5641 tctgttaact ggatcagttg ctcgatctct ttttgctgag aaactgctat ctcgtaatcc
     5701 tctcttacct tctgatctcg ttcaaaatat ttcattgcat accagcgttt cttgccgctt
     5761 tgttctccta gtacatcttc aatttcatgt aaagctgctt ctaaacgtgg atcatatgta
     5821 cagggaacag gaatagaagg ccgctttttt gcagactcct gaatcttttc gattagtttt
     5881 tcgattcctt gctttttcaa tacgctgatt ggtaaaactg gtacaccaag accataggaa
     5941 agcttttcta gattgatttt ttttccttct tttttgatga catccatcat attcagacca
     6001 atgatcatag gaatgtctaa atccaataac tgcgtagtta aataaagatt tcgttctaaa
     6061 ttggtagcat caataatgtt gatgatcagt tcaggctgtt cctgaaccaa ataatctctt
     6121 gcaatcactt cttctggtgt ataaggagac aaagaataga tgccgggtag gtcctgaata
     6181 tgaatgttcg gatttttttt caacgatcct tctttttttt ctactgttac gcccggccaa
     6241 ttccctacat attgattcga accagtcaag agattgaaca agctagtttt cccgctgttt
     6301 ggatttccga ctaacgcgat attcattctt tttcctccct ttgtatcaag attagttcgg
     6361 cctctgactt tcgtaacgtc agttcatacc ctcgcactgt cagttcgatt ggatctccca
     6421 gaggggctag ttttcggata actattttta catttcgcgt caatcccata tccattaacc
     6481 gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat cccagtctct cctgctttta
     6541 attgatcaag agaagtccag ctttcagagg aagcagtctt tttattgaca aagatttgac
     6601 ctagtatttc ccgacttact gccactcgat gactttttac cagtaaaatg gcattgttgc
     6661 ctgataaatt gacgacgacg attttacttc cggcaacgat cccgatattt tgcaaatggc
     6721 gttttgtttt ttcttctgtt tcgattcgtt cgacttgata aacgaaattc tgctttgcct
     6781 gcgctaaggt ctgcatattg attcctctct tgtgaaataa atcatgtatt aaaatcattc
     6841 attatatgtt ctaattttct tacgtgttac gcaaataaat gagaatcatt ctcacataga
     6901 atactctcgg taattgagaa tgtcaattat acttgtgata ttatgagcga atctttagta
     6961 ttgatagata aaaatgagaa aaaaatattt cttcatagtt ttccacaatt ttttctctat
     7021 taaatttgct attttcccac acgatacttg ttttcagctc ttttttcaaa aacacaaaac
     7081 ttagtattcc acagtttcca caacacaata tgtagtgttt gttgtttgac ttttttacta
     7141 agagttttat actatctatt atagataata agaatgtgtt agaggaggca ctcaccatga
     7201 acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa cggtttttaa
     7261 cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa gcagtagact
     7321 ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca gaaacagtta
     7381 tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa aagaatcaaa
     7441 acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca aacttccaaa
     7501 tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat caaatacgaa
     7561 atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt atttcaagct
     7621 gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg ataaagaagc
     7681 tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc tatctattat
     7741 ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca ttcaattgta
     7801 ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt tttctttcaa
     7861 atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca acgacggttc
     7921 cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact ttgcgaacgg
     7981 ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat atcagccagc
     8041 tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt cttgtttctt
     8101 gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg ctttgcaatt
     8161 gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag ccggcgctcc
     8221 tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat tattcgaaga
     8281 tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg tttatttgaa
     8341 cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg cagatgaaaa
     8401 gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt atgaattgac
     8461 acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag aatatggtgt
     8521 cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa atccaaacat
     8581 tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat tacaacaaga
     8641 atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc caatcgatgg
     8701 caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc cttctgtgat
     8761 taacggcaaa caggaatatg aagtattagg aacggatatc tcatgtaact taggctcgac
     8821 aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta cgatgactcg
     8881 tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga tccaaaacgg
     8941 aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt atcttgcaaa
     9001 agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt acttcatgct
     9061 gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa aacaagtttt
     9121 ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat acgtaactgg
     9181 agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt ttatcccaac
     9241 tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt atcatcaaaa
     9301 tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga gtgcaagtat
     9361 tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta aaatttatta
     9421 cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt atgatatgga
     9481 catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc aaggcatgag
     9541 tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga agacaacaac
     9601 gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca aaggaatcaa
     9661 atccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa gcaatcaatg
     9721 tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt aattaaagga
     9781 gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt gaagatatca
     9841 ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca cgtatcccac
     9901 tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat acagtaggac
     9961 atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc atggatcagt
    10021 tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt caattcatgg
    10081 aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg aaaaaagaaa
    10141 tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa gctgagcgta
    10201 tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc gtctttttag
    10261 aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga aacaacaaat
    10321 tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta catggaacct
    10381 acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa caagaaacgt
    10441 taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa cgttatacag
    10501 aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta cgctataatg
    10561 ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact gccaatgatg
    10621 tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac ttcttctcac
    10681 aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac gattatttga
    10741 ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaataa aaagagttta
    10801 gcgtacagat attttaaatg tacactgaac tcttttttat gcttattcac ttgtttcttc
    10861 cagcactttt ggactgtcta acttttccga aacatgttcc attcttctca ataaatagat
    10921 tttccggata tttaatatta tcgtcttaat cactgcatag acaggtatgc aaatcagcat
    10981 gcctaatatt cctgcgacac ttcctgctcc aatcaatacc aagatgatcg tcaaaggatg
    11041 gatattcatt gatttcccaa ataacaacgg cttgatcaaa tttccatcta attgttggat
    11101 cactaaaatc gacaacgcca tatacaaagc ttgaaatgga gagataaaca aaccgacaat
    11161 gattgctggt accgctccaa taaaagggcc gacatacggg ataatattcg tgattccgca
    11221 aatgaccgca agcaacaagc tataaggctg atcgaatatt ctcattgcca aaaagctcat
    11281 tacaccaata atcaaagcat ctaagatcgt actgctgata taggcagata atgtttcgtt
    11341 taattctttt accgtttggc gtaactcttt acgaatcgct ttcggaaaga agtgagacaa
    11401 cgcatcaata aatttgtgcc catccttgaa cataaatagc aaaataaatg gaacggtaaa
    11461 taacaaaata aaaaattgca tcataaatga aatgattttt gagacacttg cagataaact
    11521 gactaatatg atattgaaca aattactaag agataaattt gctgatgcta attgttcttc
    11581 caaattgaaa tttttaaact cttcccgctg cgccaattca tttaaccatt tgcttgtttc
    11641 atctgcatac attggcaagc tctgggtcaa ttgaattgtc tgttccatta actgaggcac
    11701 tacattcatg acagttaaaa caactaaaat gattacgacc aaaaacgtca ataaaaaacc
    11761 gaacactctc gggatccgat gcttttcgag aaaaacaacg attggatcga acatataata
    11821 gataaaacca gcaacaagca atggtaaaaa aactgccgaa agcattttca atattggact
    11881 aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta ctagctccac
    11941 tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc tccaaggtcc
    12001 tttgatactt ctattctatc atttaacaaa gagttacgcg ctgagatttt catttgctta
    12061 aaaaaaacaa aaaaaataaa catattttaa tgagtagacg agtacttaaa caaaaacacg
    12121 gtttggagta gtgacaaaaa ttatactgca gtcccttttt ccaaataaac ggtgttcaaa
    12181 agcttgcatc ttcgtaataa gcccagccaa acgaaaaata taaggaacta tttttatttg
    12241 gctgggctta ttacttgatg taggacatct attttacaac ctcagatcat ttattgcgca
    12301 atactgacta aatgcaactt tgtgatattt ttgtgtacgg acaactcctc gatcacatcg
    12361 gcataagtga gcccttttgg aagatcaatc gtatagatat ttttgtacac tcggtagtca
    12421 tcaacgaatt ttacgtcaaa attcacatct tcgattacga tatttttctc ttcaaaataa
    12481 tcggtaagaa aatctttggt ttcttttcta tggataaatt ggatttctaa ctttttagtc
    12541 gtaggtacat ggatcacgcg tttcactact gtgagtgcaa tagcaatccc gataaaactg
    12601 atgatcgcaa tggaatagta acccatgccg atggcgatcc ctaatccagc aattgcccat
    12661 aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg
    12721 cctaagaaac cgacaccact caccacttga gcaatcagcc ttgcttcatc tgaacgaatc
    12781 gttccagcaa gctgtggata ctctaaagca tctcttaatg caccactagc gatctcaact
    12841 tggatcaatg caatgataca tgctcccata caaactaaaa tatgcgtacg catacctgct
    12901 ggacgatttt tatattgtcg ttcaaaaccg attgctccgc caaatagcat agcgatccct
    12961 aaacgtaaaa taatctctgg aattgtcaat accatatcat tcatctaaac accctccagt
    13021 ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa
    13081 aaaggactgt gacaaaattt ttgtcatagt cctttttatg aataaacggt gttcaatcag
    13141 ctgaccctcg gtattactca acaaacggca aaatatgggg agctattttt cctttgtctt
    13201 ctctaatact caggtcaaaa cgctgatttc acaacctctt tagcctacta ttgaaaatta
    13261 caacttactt gctgcttctt catcgatgat cacaacaaca ttgtcgtgat tttgcaatac
    13321 acttgctggc aaatgattcg ttacaggtcc ttcaatcatc cctttgattg catcagcttt
    13381 gctttcgcca aatgccatca agacgatttc tttacttttc ataattgaac cgattcccat
    13441 tgaaacagct tttgttggta catcttctgc tttatcgaag aatcgtttgt ttgcattgat
    13501 tgttgattct gttaagtcaa ctactgaagt cgttccgtca aatgaagcac caggttcatt
    13561 gaaaccgata tgtccatttt gcccgattcc taagatttgg atatcgattg gatgtgcatc
    13621 gatgatttga tcatactttt tgcatgctgc ttccagatct tctgcttttc catctggtac
    13681 aaacgtttct ttgaatggtt ttttatcaaa taattggaca tccatgaaat gacggtagct
    13741 ttgttcatct gaaccgccta gtccaacgta ctcatctaag tttacagaag tcatttcact
    13801 gaaatccaaa tcgcttgaag tcatttcttt atataaagta actggtgtgc ttcctgttgc
    13861 caatcctaat acttttgcac ctttgttcat accttctttg atgatttcaa atgctttttt
    13921 tccgccttct tcagcgtttt ttacacgaat gatttccata gtgattgctc ctttatattt
    13981 tggtatagcc caattatagc attctttttt tctttttaca ataattagta tagaccaaat
    14041 tgcatttttt tcattttctt gacaataaat aggaatccgt gttaggtttc ataagatggt
    14101 tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg attcactgtg gacgctttat
    14161 gtctgcagtt tttttatgaa aaaggagtta ttatgagtat actgacatta aacaacatta
    14221 cccagcaatt tggcgacaaa atcctctatg aaggtgtcga tctgcaatta aatgctggcg
    14281 aacatcttgg gctgattgga caaaacggag ccggtaaaag tacattgatt aaaatcatca
    14341 ctggtgagat cttaccagat gacggacaga tccattggca aaaaaatatt cacaaaggtt
    14401 acctcgatca atatgtggaa gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg
    14461 cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt tctttaccaa acatatagcg
    14521 agaccttcga tgattccttg ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag
    14581 gagtgtttta tcaaatcgac accttagttg ctgagatgag cagcgggttg gggatcgatg
    14641 tcttaggttt agattcactg ttgaaaaatc taagtggtgg acaacgttca aaggtgattt
    14701 tagcaaaact acttttagaa aacccagatg ttctgatctt agatgaacca accaatcact
    14761 tagatgatca gcatatccaa tggctgacgc agtttcttca ggattttgac ggtgcgtata
    14821 tcgtgatttc ccatgaccaa gaatttcttg atcagatcac cacccatatc gcagacatcg
    14881 agtttggaaa gattactaag tatactggcc atctaaaaca agcattgaaa caaaaggaac
    14941 aaaatcgcga aagctatttg cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg
    15001 aagcttatat tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa agccgacaaa
    15061 aacaattaga taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa gctgtctttg
    15121 attttccatt ctcgcctatt gtcacgacat tagcagtgga aacaactgat ttagtcatcg
    15181 gatatgatcg accgctactt gcacccatca atttgaccat ccgctttggt gaaaatattg
    15241 ccattcgagg cttcaacggg attggaaaat ctactttatt aaaaacactg atcggtgaga
    15301 ttgaaaaaat aagtggcgat ttccacttcc cagataacac aaaaatcaat tacttttcac
    15361 aagatctgta ctgggaaaat ccattagaaa caccactaca atatttaagc agtcaatttc
    15421 caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa agcagggtta gttaatcaat
    15481 tagcaagtga acctctcact acattaagtg gtggggaaca aactaaggtg aaacttgcac
    15541 agttgacaat gaaccaaggt aatttactga ttctcgatga accgacgaac catatcgatc
    15601 aagaaacgaa agaaagctta caagacggga tacagaaatt tccaggaaca gtaatcatcg
    15661 tctctcatga acaagaattt tatcaagatc ttgtgcaacg agtcattgag attgaaccaa
    15721 aataaacgtt tgattgaata gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact
    15781 ccttcttctg aataaatggt ggccaattag ctgaccttcg gtattagctc aaaaaacggc
    15841 aaaatatgag gagctatttt tatttggcta ttcttattac ttgatgcaga acagcttttc
    15901 acaacctctt ttcgtctatt ttttattttc gagatttctc tttcctttaa aaacaactgt
    15961 ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta accgttacac ctaaccagcc
    16021 atactgctgc cacattgtcg tccctagcaa tgaaccagcc gcacctccaa taaagtacat
    16081 aaacataaat acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt
    16141 ttgattggcg acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa
    16201 aatggccaaa tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag
    16261 tcccatatac acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc
    16321 agcaactgct ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt
    16381 gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa
    16441 catgaaaaat ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg
    16501 caaactttta agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg
    16561 cattttccag taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat
    16621 catccgccaa gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc
    16681 cacgagtaaa ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag
    16741 taccgcaccg taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga
    16801 agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga
    16861 taacgctgcc attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa
    16921 aaacagtaac cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact
    16981 tgtgctaaca gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt
    17041 ggcaacaaca acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggttt
    17101 tttttcatta ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa
    17161 agattggaaa tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc
    17221 ttgttttttc ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact
    17281 tgcaaccaac gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc
    17341 atacaacaca gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg
    17401 caatttcatc gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata
    17461 gcctaacatt acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt
    17521 cgtttcggtt ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt
    17581 accaagagca ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc
    17641 agcaatgact ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa
    17701 tacgatcaag agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc
    17761 cttactcgct ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg
    17821 atagcccact accagtatca gaatgattcc caacgagatc aatatcaatc ggattatttt
    17881 ctttttcata tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa
    17941 acaaaaaagc accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt
    18001 aacagttaca gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt
    18061 agatacgcct tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa
    18121 gcctaaacca gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc
    18181 agttaattct acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc
    18241 tacagctaca ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc
    18301 gttaagttct aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc
    18361 cattataatt tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag
    18421 ctgcatctcc atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg
    18481 gcgcttgtag tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca
    18541 atgctgtgat ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta
    18601 atgcttttgc ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt
    18661 tactgaatgc aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt
    18721 ttttagctgc acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac
    18781 gaagttgttt acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta
    18841 ctactgatgc agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg
    18901 cgataattgc ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt
    18961 gcactgaaaa ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct
    19021 attcacttcg ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg
    19081 ctcaaccagt attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact
    19141 ctatgccacc gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga
    19201 aattaaggct tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc
    19261 ttagcatgct aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta
    19321 atatttaatt agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc
    19381 gttacatttt tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca
    19441 tggattgctg caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt
    19501 acatggatat ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt
    19561 actgcttttg ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt
    19621 ggtcctaata ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact
    19681 acatcaaagt caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa
    19741 tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt
    19801 tgtgttttac cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt
    19861 ttagggtcta cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt
    19921 gtttcttttg ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt
    19981 tttaatgcat cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg
    20041 ttataacggt tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc
    20101 tgctgtctta gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc
    20161 aaccatgcgc attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc
    20221 gatttctctt acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga
    20281 acctttttcg attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac
    20341 gaatgtgaat gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg
    20401 atcagctgta cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc
    20461 tagtgcagga cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac
    20521 gattttttct actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt
    20581 ggagatgcat atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg
    20641 tgcaaaacaa tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt
    20701 ctgctatttt tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt
    20761 ttcgttctca tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc
    20821 aaacaataac acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg
    20881 gagaaaatga aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt
    20941 ggtccaagta gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata
    21001 tttggcgaac agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat
    21061 cgggggcacg gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat
    21121 gaacgtttag ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt
    21181 cctaagagtg aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca
    21241 cgaaagcttt cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg
    21301 aatggattca aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat
    21361 gttccaggta tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc
    21421 actatgacag tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat
    21481 gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta
    21541 aattatttcg attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa
    21601 gcaaaagctt atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc
    21661 cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca
    21721 atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa
    21781 aaattagatg attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct
    21841 gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca
    21901 ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt
    21961 gatttgacac atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc
    22021 atcgcaaaca acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct
    22081 gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca
    22141 gcacaagcag tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca
    22201 ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc
    22261 tctgctgata tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct
    22321 gcaatgtacc aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc
    22381 ttagctgtta cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga
    22441 taaagctcct taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag
    22501 aggactaatt gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc
    22561 atcgatcgtt tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc
    22621 aaaaacagat gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc
    22681 aatcgcaact aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac
    22741 accagagtcg aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac
    22801 ttcaaaggac gcaggacctc catctagttg acgtccacca tgattggata cccagatacc
    22861 atctgcccct gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat
    22921 aactggaaga tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt
    22981 tgcagctgca taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc
    23041 gctatacgca gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc
    23101 ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc
    23161 tactgctttg ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa
    23221 aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt
    23281 gctgatggaa aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt
    23341 ttcatgtgcc aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat
    23401 tccgaaaaca gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat
    23461 tttctttgta ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc
    23521 gcgaatatag ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc
    23581 aatgtttaca atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc
    23641 ttctttcatc ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat
    23701 ttactctcag atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta
    23761 cttcttggtt ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct
    23821 ctatttcatt tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta
    23881 ctgttccatc actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg
    23941 ttataggaat attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag
    24001 gcccctttgc taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag
    24061 gcgcgccaat tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc
    24121 ctaaatagcg tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta
    24181 tgccgtagtt ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact
    24241 cgttattttt attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca
    24301 aatctcctgt tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg
    24361 cttgctgatt tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt
    24421 agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg
    24481 ttttttcttt atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg
    24541 caaacaaact gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg
    24601 gttctgtcat acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca
    24661 tatgatcgac atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt
    24721 tctgtactgg tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg
    24781 tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact
    24841 tcgccaactt ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata
    24901 tgattgactt cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg
    24961 aatcctgtca caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt
    25021 tcaacaagta cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg
    25081 acttcagttt ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata
    25141 ctttgcgcac gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc
    25201 aagacatacc agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata
    25261 aaaaaacctc agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt
    25321 gtggctagcg agttatttta aaatccagcc aaataagctt tggatggctg tatccattac
    25381 gtaaaacatc aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag
    25441 ttgttttctg cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt
    25501 catgagacaa cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta
    25561 caatatttac aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat
    25621 tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt
    25681 tacttctgcc cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt
    25741 tacttgtcaa tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa
    25801 tttgaaatta agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt
    25861 atataagaaa aaagatctga caaccgttac agagactagt acattagaag aagcattaaa
    25921 aattttagaa acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt
    25981 ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac
    26041 gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt
    26101 cttcaaagta ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa
    26161 tcatttttat ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag
    26221 cgtagaacaa ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc
    26281 tgctattgca aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat
    26341 cgaaaaagac gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa
    26401 agtatgtgaa acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga
    26461 aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt
    26521 tcttttttcg ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa
    26581 tacatcaatg gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag
    26641 gttgtccaaa atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg
    26701 atggtaaaga agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc
    26761 aataaagctg tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg
    26821 ataccaaaac caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat
    26881 ttacggttaa agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct
    26941 gaggatacag gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt
    27001 acaacgatta cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc
    27061 tcctagtttt tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat
    27121 tataacagac ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt
    27181 aaacaatgta aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc
    27241 ataagaattc ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac
    27301 ttaaataaat aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc
    27361 aagcagctga agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat
    27421 ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc
    27481 tagacttctg ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag
    27541 tttctttcat tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag
    27601 tattaaaata tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat
    27661 attgatcaat ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc
    27721 ggtattaaaa aataagcata ttgactatac acttcattat agtgaataca aaggacacga
    27781 tgctgagatt gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca
    27841 gaaaacaatc gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca
    27901 ggtacttgat actttctatc agatggaagt agaattccca gttgcttata ttccagcagg
    27961 ttctggagat gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca
    28021 gctgatttta gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat
    28081 aagtgaaaaa aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt
    28141 ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt
    28201 ttcgtattta ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt
    28261 agatgcagga ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca
    28321 tccttttttt ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat
    28381 tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact
    28441 tatacagaaa aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg
    28501 tatcgtttca acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc
    28561 ttttgatatc acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg
    28621 tcagaagcta catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt
    28681 cacagtcctt tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt
    28741 tctttcttta cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac
    28801 acaaccattg aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat
    28861 caagttatta ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat
    28921 ttctggcaag tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa
    28981 aatatctttg atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga
    29041 aaagctaatg atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat
    29101 atcccacgag gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag
    29161 cagcgagtaa ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg
    29221 cgcactctgg tttcgtttac tgatctcttt gcgattaaat gctagattga tgacgctgaa
    29281 accgctaaaa atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa
    29341 cagctttttc atccgctgac tatatcccgc acttagctca acttctccag taaccgagag
    29401 tgccatttgt gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat
    29461 cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg
    29521 gcgactctgt atcttgatcg tcggcatctt tcctacttcg acttgttcgg taaagtctgc
    29581 aggcagatgt aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg
    29641 tgaggttcca tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc
    29701 gactacaggc gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt
    29761 ttcaacatca atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat
    29821 cagtaaattc gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac
    29881 gcacccctct ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc
    29941 atagaagatt ggtataaaaa ctagccaaat cagtgtagaa ataaagtt
//
LOCUS       DACDBG010000028        29981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDBG010000028 DACDBG010000000
VERSION     DACDBG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.028"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            137..475
                     /locus_tag="IVZ90_001251"
     CDS             137..475
                     /locus_tag="IVZ90_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262315.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="IVZ90_001252"
     CDS             complement(680..1255)
                     /locus_tag="IVZ90_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8262316.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="IVZ90_001253"
     CDS             complement(1987..2568)
                     /locus_tag="IVZ90_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8262317.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="IVZ90_001254"
     CDS             complement(2751..3377)
                     /locus_tag="IVZ90_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8262318.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="IVZ90_001255"
     CDS             complement(3399..4730)
                     /locus_tag="IVZ90_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8262319.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="IVZ90_001256"
     CDS             complement(4885..5286)
                     /locus_tag="IVZ90_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8262320.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="IVZ90_001257"
     CDS             complement(5372..5578)
                     /locus_tag="IVZ90_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262321.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="IVZ90_001258"
     CDS             complement(5673..7175)
                     /locus_tag="IVZ90_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8262322.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="IVZ90_001259"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="IVZ90_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8262323.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="IVZ90_001260"
     CDS             complement(7746..8363)
                     /locus_tag="IVZ90_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262324.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="IVZ90_001261"
     CDS             8584..9957
                     /locus_tag="IVZ90_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8262325.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="IVZ90_001262"
     CDS             10032..11144
                     /locus_tag="IVZ90_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8262326.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="IVZ90_001263"
     CDS             complement(11216..12853)
                     /locus_tag="IVZ90_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8262327.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="IVZ90_001264"
     CDS             complement(13021..13629)
                     /locus_tag="IVZ90_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8262328.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14013..15164)
                     /gene="queG"
                     /locus_tag="IVZ90_001265"
     CDS             complement(14013..15164)
                     /gene="queG"
                     /locus_tag="IVZ90_001265"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8262329.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15183..15935)
                     /locus_tag="IVZ90_001266"
     CDS             complement(15183..15935)
                     /locus_tag="IVZ90_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262330.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16076..17026)
                     /locus_tag="IVZ90_001267"
     CDS             complement(16076..17026)
                     /locus_tag="IVZ90_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8262331.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17027..17866)
                     /locus_tag="IVZ90_001268"
     CDS             complement(17027..17866)
                     /locus_tag="IVZ90_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8262332.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17863..18609)
                     /locus_tag="IVZ90_001269"
     CDS             complement(17863..18609)
                     /locus_tag="IVZ90_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8262333.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18847..20217
                     /locus_tag="IVZ90_001270"
     CDS             18847..20217
                     /locus_tag="IVZ90_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8262334.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20272..21168)
                     /locus_tag="IVZ90_001271"
     CDS             complement(20272..21168)
                     /locus_tag="IVZ90_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8262335.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21449..22126)
                     /locus_tag="IVZ90_001272"
     CDS             complement(21449..22126)
                     /locus_tag="IVZ90_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8262336.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22217..23122)
                     /locus_tag="IVZ90_001273"
     CDS             complement(22217..23122)
                     /locus_tag="IVZ90_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8262337.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23297..24751
                     /locus_tag="IVZ90_001274"
     CDS             23297..24751
                     /locus_tag="IVZ90_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262338.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24767..25798
                     /locus_tag="IVZ90_001275"
     CDS             24767..25798
                     /locus_tag="IVZ90_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8262339.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25805..26992
                     /locus_tag="IVZ90_001276"
     CDS             25805..26992
                     /locus_tag="IVZ90_001276"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8262340.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27148..28530
                     /locus_tag="IVZ90_001277"
     CDS             27148..28530
                     /locus_tag="IVZ90_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8262341.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28502..28888
                     /locus_tag="IVZ90_001278"
     CDS             28502..28888
                     /locus_tag="IVZ90_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262342.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29088..29756)
                     /locus_tag="IVZ90_001279"
     CDS             complement(29088..29756)
                     /locus_tag="IVZ90_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8262343.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6139 c         5483 g         9955 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    13741 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    13801 ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat
    13861 tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca
    13921 aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaaa acaattactg
    13981 gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg caataatttc
    14041 tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct
    14101 aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct
    14161 gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt
    14221 cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc
    14281 catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt
    14341 ggctttaatt ttggatatac ttcgtctact tttggttcca tctcttcatg aaaatgaaaa
    14401 tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt
    14461 cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa
    14521 catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg
    14581 cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta
    14641 gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt
    14701 cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta
    14761 tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg
    14821 atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa
    14881 gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta
    14941 ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa
    15001 agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct
    15061 tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt
    15121 cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc
    15181 tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc
    15241 tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa
    15301 taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact
    15361 ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga
    15421 tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc
    15481 gggattttcc caggcctagc aatttctact agacgattat aaaacatatt cattaatttg
    15541 ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat tactccttct
    15601 gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta
    15661 actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta
    15721 ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct
    15781 ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat
    15841 tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct
    15901 ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta
    15961 actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt
    16021 tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc
    16081 tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat
    16141 agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga
    16201 ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca
    16261 cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag
    16321 gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc
    16381 gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag
    16441 aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg
    16501 catttttttt atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt
    16561 cctccacata ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat
    16621 gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt
    16681 ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa
    16741 gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg tcttctggta
    16801 gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat
    16861 ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact
    16921 tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca
    16981 agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt
    17041 tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac
    17101 atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga
    17161 caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg
    17221 ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta
    17281 ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca
    17341 gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt
    17401 ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca
    17461 aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac
    17521 tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa
    17581 tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta
    17641 gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta
    17701 cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa
    17761 taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt
    17821 gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc
    17881 ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac
    17941 ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata
    18001 ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa
    18061 ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc
    18121 atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag
    18181 ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt
    18241 caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga
    18301 tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc
    18361 acttcgctgt tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa
    18421 ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt
    18481 tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat
    18541 agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt
    18601 acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta
    18661 actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg
    18721 cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat
    18781 gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt
    18841 ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc
    18901 gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg
    18961 acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg
    19021 cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg
    19081 agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat
    19141 attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct
    19201 gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt
    19261 atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg
    19321 atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt
    19381 caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa
    19441 ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga
    19501 atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact
    19561 atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg
    19621 tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt
    19681 acatttgctg caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat
    19741 tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct
    19801 aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca
    19861 ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca
    19921 gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta
    19981 gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat
    20041 ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc
    20101 gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt
    20161 ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa
    20221 aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa
    20281 ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa
    20341 gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa
    20401 aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt
    20461 tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga
    20521 atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg
    20581 caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat
    20641 caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt
    20701 tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga
    20761 taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc
    20821 aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg
    20881 caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc
    20941 acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc
    21001 ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa
    21061 cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct
    21121 tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac
    21181 caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg
    21241 tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc
    21301 caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc
    21361 agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca
    21421 tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg
    21481 taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt
    21541 gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt
    21601 caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta
    21661 gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa
    21721 gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg
    21781 tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga
    21841 ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa
    21901 tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat
    21961 gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt
    22021 ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta
    22081 aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt
    22141 tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc
    22201 attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt
    22261 tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg
    22321 tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc
    22381 ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc
    22441 gattgatccg taccccgatc aaatattcct tgatactggt acccatatga tttttgaata
    22501 ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt
    22561 tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct
    22621 tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg
    22681 ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta
    22741 aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga
    22801 aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca
    22861 tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca
    22921 gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca
    22981 tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg
    23041 ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt
    23101 ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa
    23161 aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag
    23221 tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag
    23281 gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc
    23341 ttttggacac gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag
    23401 ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag
    23461 cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat
    23521 cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt
    23581 tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac
    23641 gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt
    23701 taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga
    23761 aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt
    23821 cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc
    23881 ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat
    23941 cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg
    24001 gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc
    24061 ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct acttcttcaa
    24121 atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg
    24181 tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta
    24241 ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag
    24301 tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt
    24361 atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg
    24421 taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc
    24481 taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc
    24541 tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc
    24601 tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca
    24661 tgcacaaatc gttgaggaat tggaaaacaa attggctcca gctgatacaa tggaagatga
    24721 acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga
    24781 aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact
    24841 ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt
    24901 tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat
    24961 cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca
    25021 attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt
    25081 aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac
    25141 tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac
    25201 tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt
    25261 attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga
    25321 tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc
    25381 tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt
    25441 agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc
    25501 atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa
    25561 aagaatccaa gtaggtattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt
    25621 cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt
    25681 tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa
    25741 agctggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact
    25801 catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa
    25861 aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa
    25921 tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg
    25981 agacgataat aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt
    26041 tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat
    26101 ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt tgttagtgga
    26161 aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg
    26221 tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg
    26281 taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat
    26341 cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat
    26401 ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga
    26461 attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt
    26521 gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac
    26581 tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa
    26641 tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca
    26701 actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt
    26761 agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg
    26821 agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa
    26881 aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc
    26941 acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa
    27001 tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg
    27061 agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt
    27121 tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat
    27181 gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta
    27241 gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg
    27301 tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa
    27361 aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt
    27421 gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat
    27481 tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc
    27541 ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct
    27601 aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc
    27661 gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg
    27721 tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact
    27781 gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct
    27841 gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt
    27901 actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca
    27961 ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa
    28021 gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct
    28081 gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca
    28141 aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat
    28201 ctagcagatg ctttattgac cattttccac cagcattacc actaccagga tattcggcgt
    28261 gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa
    28321 gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa
    28381 aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc
    28441 gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa
    28501 gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg
    28561 attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga
    28621 agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag
    28681 tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga
    28741 tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga
    28801 ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa
    28861 ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa
    28921 ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt
    28981 cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt
    29041 agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt
    29101 cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac
    29161 cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata
    29221 agccatgctt cagtcaattg attggactga agcactttat gctgaatttg cccttcatct
    29281 acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact catttgacca
    29341 gcattctcta ggataacatg tttggcattt tcaagagaaa agcattcttg ctgtcttaag
    29401 attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg
    29461 ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt
    29521 tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca
    29581 tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc
    29641 gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc
    29701 actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt
    29761 tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat
    29821 atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc
    29881 aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat
    29941 atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DACDBG010000029        27671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDBG010000029 DACDBG010000000
VERSION     DACDBG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.029"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..802
                     /locus_tag="IVZ90_001280"
     CDS             155..802
                     /locus_tag="IVZ90_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8262344.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            886..1830
                     /locus_tag="IVZ90_001281"
     CDS             886..1830
                     /locus_tag="IVZ90_001281"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8262345.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1965..3416
                     /locus_tag="IVZ90_001282"
     CDS             1965..3416
                     /locus_tag="IVZ90_001282"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8262346.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3437..4273
                     /locus_tag="IVZ90_001283"
     CDS             3437..4273
                     /locus_tag="IVZ90_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8262347.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4273..4932
                     /locus_tag="IVZ90_001284"
     CDS             4273..4932
                     /locus_tag="IVZ90_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8262348.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLNWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5058..5747
                     /locus_tag="IVZ90_001285"
     CDS             5058..5747
                     /locus_tag="IVZ90_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262349.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5753..6709
                     /locus_tag="IVZ90_001286"
     CDS             5753..6709
                     /locus_tag="IVZ90_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8262350.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6753..7265
                     /locus_tag="IVZ90_001287"
     CDS             6753..7265
                     /locus_tag="IVZ90_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8262351.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7521..8069)
                     /locus_tag="IVZ90_001288"
     CDS             complement(7521..8069)
                     /locus_tag="IVZ90_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8262352.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8158..9339)
                     /locus_tag="IVZ90_001289"
     CDS             complement(8158..9339)
                     /locus_tag="IVZ90_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8262353.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9575..11083)
                     /locus_tag="IVZ90_001290"
     CDS             complement(9575..11083)
                     /locus_tag="IVZ90_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8262354.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11242..11433
                     /locus_tag="IVZ90_001291"
     CDS             11242..11433
                     /locus_tag="IVZ90_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262355.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11636..12553
                     /locus_tag="IVZ90_001292"
     CDS             11636..12553
                     /locus_tag="IVZ90_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8262356.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12546..13619
                     /locus_tag="IVZ90_001293"
     CDS             12546..13619
                     /locus_tag="IVZ90_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262357.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13767..14285
                     /locus_tag="IVZ90_001294"
     CDS             13767..14285
                     /locus_tag="IVZ90_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262358.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14266..14658
                     /locus_tag="IVZ90_001295"
     CDS             14266..14658
                     /locus_tag="IVZ90_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8262359.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14734..15669
                     /locus_tag="IVZ90_001296"
     CDS             14734..15669
                     /locus_tag="IVZ90_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8262360.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15802..16581
                     /locus_tag="IVZ90_001297"
     CDS             15802..16581
                     /locus_tag="IVZ90_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8262361.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16574..16891
                     /locus_tag="IVZ90_001298"
     CDS             16574..16891
                     /locus_tag="IVZ90_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8262362.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17135..18745
                     /locus_tag="IVZ90_001299"
     CDS             17135..18745
                     /locus_tag="IVZ90_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8262363.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18902..19750
                     /locus_tag="IVZ90_001300"
     CDS             18902..19750
                     /locus_tag="IVZ90_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8262364.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19981..20460
                     /locus_tag="IVZ90_001301"
     CDS             19981..20460
                     /locus_tag="IVZ90_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262365.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20493..22721
                     /locus_tag="IVZ90_001302"
     CDS             20493..22721
                     /locus_tag="IVZ90_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8262366.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22850..24493
                     /locus_tag="IVZ90_001303"
     CDS             22850..24493
                     /locus_tag="IVZ90_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8262367.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24566..25111)
                     /locus_tag="IVZ90_001304"
     CDS             complement(24566..25111)
                     /locus_tag="IVZ90_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8262368.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25134..25619)
                     /locus_tag="IVZ90_001305"
     CDS             complement(25134..25619)
                     /locus_tag="IVZ90_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8262369.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25609..26421)
                     /locus_tag="IVZ90_001306"
     CDS             complement(25609..26421)
                     /locus_tag="IVZ90_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8262370.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26560..>27671)
                     /locus_tag="IVZ90_001307"
     CDS             complement(26560..>27671)
                     /locus_tag="IVZ90_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8262371.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9059 a         5035 c         5531 g         8046 t
ORIGIN      
        1 gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa
       61 aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat
      121 agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag
      181 tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg
      241 gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc
      301 aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat
      361 caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat
      421 tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga
      481 tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa
      541 tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga
      601 tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag
      661 cggtacggca acagattggg atttggataa agatttaggt actacttatt gggaagtcaa
      721 agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact
      781 gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag
      841 tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg
      901 acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg
      961 actacccagg aattagatga actggctcat tcagaagcag gagcatttat cactaagagc
     1021 tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg
     1081 agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt
     1141 gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg
     1201 agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc
     1261 actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt
     1321 gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc
     1381 aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag
     1441 tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca
     1501 gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat
     1561 atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc
     1621 caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt
     1681 ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga aattttttct
     1741 agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa
     1801 tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg
     1861 tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt
     1921 agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag
     1981 aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac
     2041 agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg
     2101 agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc
     2161 catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc
     2221 gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga
     2281 taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag
     2341 acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat
     2401 caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg
     2461 atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt
     2521 cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg
     2581 atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta
     2641 tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa
     2701 aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg
     2761 cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag
     2821 agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg
     2881 cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag
     2941 aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta
     3001 cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc
     3061 aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag
     3121 atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag
     3181 aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac
     3241 agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa
     3301 acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa
     3361 tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga
     3421 aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt
     3481 tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt
     3541 agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta
     3601 taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg
     3661 gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc
     3721 aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa
     3781 attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga
     3841 tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa
     3901 tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc
     3961 cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga
     4021 ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga
     4081 gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct
     4141 gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat
     4201 atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc
     4261 ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct
     4321 ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga
     4381 aatctggtag ggttgctttt gacagtttta ggtcttttga attggctgcc tctgaatgta
     4441 tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg
     4501 ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg
     4561 attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
     4621 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
     4681 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
     4741 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
     4801 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
     4861 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat
     4921 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
     4981 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
     5041 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
     5101 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
     5161 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
     5221 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
     5281 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
     5341 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
     5401 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
     5461 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
     5521 gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa
     5581 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
     5641 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
     5701 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
     5761 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
     5821 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
     5881 aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca
     5941 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
     6001 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
     6061 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
     6121 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
     6181 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
     6241 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
     6301 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
     6361 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
     6421 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
     6481 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
     6541 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
     6601 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
     6661 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
     6721 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
     6781 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
     6841 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
     6901 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct
     6961 caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
     7021 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
     7081 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
     7141 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
     7201 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
     7261 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
     7321 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
     7381 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
     7441 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct
     7501 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct
     7561 tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac
     7621 tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca
     7681 cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact
     7741 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
     7801 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
     7861 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
     7921 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
     7981 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
     8041 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
     8101 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta
     8161 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
     8221 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
     8281 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
     8341 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
     8401 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
     8461 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
     8521 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
     8581 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
     8641 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
     8701 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
     8761 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
     8821 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
     8881 tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc
     8941 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
     9001 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
     9061 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
     9121 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
     9181 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
     9241 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
     9301 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
     9361 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
     9421 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
     9481 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
     9541 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
     9601 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
     9661 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
     9721 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
     9781 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
     9841 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
     9901 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
     9961 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
    10021 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
    10081 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
    10141 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
    10201 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
    10261 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
    10321 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
    10381 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
    10441 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
    10501 ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag
    10561 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
    10621 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
    10681 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
    10741 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
    10801 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
    10861 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
    10921 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc
    10981 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
    11041 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
    11101 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
    11161 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
    11221 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
    11281 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
    11341 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
    11401 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
    11461 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
    11521 tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg
    11581 gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa
    11641 agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga atgcggatca
    11701 ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct
    11761 ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc
    11821 tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga
    11881 aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt
    11941 tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac
    12001 ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt
    12061 gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa
    12121 aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga
    12181 tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt
    12241 gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc
    12301 taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa
    12361 agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga
    12421 acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga
    12481 attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc
    12541 cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc
    12601 atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag
    12661 aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact ccgacgaata
    12721 cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag
    12781 gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag
    12841 cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat
    12901 atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg
    12961 aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt
    13021 cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa
    13081 acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga
    13141 tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc
    13201 tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat
    13261 tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc
    13321 agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga
    13381 cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg
    13441 aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa
    13501 caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg
    13561 tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat
    13621 tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag
    13681 ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg
    13741 ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag cttagcttat
    13801 tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat ttccagccaa
    13861 tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg ttaattgtca
    13921 tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg gtcatcggcc
    13981 aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg agaaaaacag
    14041 tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg gtttacggaa
    14101 taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg atctattacc
    14161 ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt
    14221 ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa aaaaataatt
    14281 tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag tacaacctat
    14341 acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg
    14401 ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat
    14461 ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt catcacttcg
    14521 cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac tgtggacggg
    14581 aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc ggtcattttc
    14641 aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt gccataattc
    14701 agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa aagcggaatg
    14761 ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa atttgtcggc
    14821 tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat cgttgattgc
    14881 agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca gagcgagttg
    14941 catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc aatgatgctt
    15001 ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac gcatccatcc
    15061 catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt ggtaatcgaa
    15121 ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga aaaactttcc
    15181 acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt tacggaagac
    15241 ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac ttatgcaacc
    15301 gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct gatgggagcc
    15361 gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt gacagcaagc
    15421 gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct gatcgatata
    15481 aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga tatcgttggt
    15541 aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct
    15601 cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc aaattattct
    15661 cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata atcttgtttt
    15721 taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg tttatttgtt
    15781 gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct tcaagctttg
    15841 catgcagtga tgccactttg tataagctat ataccagtag gattagcttg cggagtattg
    15901 ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct ggtcttttcc
    15961 ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt tgcaacgaca
    16021 ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag tcttgccggt
    16081 tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact gaatgatgaa
    16141 aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa aagaaaggct
    16201 ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat cggtaatttt
    16261 ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac ggccatgttc
    16321 attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg tttattctca
    16381 ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt gattgcagca
    16441 acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa agaaaaagct
    16501 aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt cccggaacag
    16561 gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc tgttcatcgt
    16621 ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag tgccatcctg
    16681 gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag cttttaaaga
    16741 tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag ctgcaatgat
    16801 tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt tgacaggact
    16861 tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa aacagagatt
    16921 gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc aattctggat
    16981 gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa gctctttttt
    17041 attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat agggaagatg
    17101 tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac
    17161 attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct atttgattcc
    17221 ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat tgtataatgt
    17281 tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc caaatgcgat
    17341 tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc ggctgatttt
    17401 tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt
    17461 atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg ggatactagc
    17521 tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa tgagaggata
    17581 tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg aacaggctgt
    17641 gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg acggaaccat
    17701 attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag ctgcgattat
    17761 ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc
    17821 cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc gggaaacact
    17881 accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc aagtaacgat
    17941 gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg aattcttatt
    18001 cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca tggtaggaac
    18061 agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc ggatacaaat
    18121 cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc cttcattgac
    18181 tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg atcctgaaag
    18241 tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat tcacgatcct
    18301 atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa ctgtagaagc
    18361 aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg gatacgggat
    18421 gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc actttatgtg
    18481 caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa aaactttccg
    18541 ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga atcagcgatt
    18601 gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg
    18661 cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga aaaatgttaa
    18721 acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca cttgaaaaac
    18781 ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa
    18841 tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca
    18901 catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca
    18961 tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg
    19021 aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac
    19081 tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg
    19141 gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt
    19201 atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac
    19261 tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc
    19321 ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg cttatctgat
    19381 tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat
    19441 tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt
    19501 cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag
    19561 aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt
    19621 agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg
    19681 tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc
    19741 agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat catcttggac
    19801 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
    19861 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
    19921 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
    19981 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
    20041 gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag
    20101 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
    20161 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
    20221 gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
    20281 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
    20341 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
    20401 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
    20461 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
    20521 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
    20581 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
    20641 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
    20701 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
    20761 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
    20821 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
    20881 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
    20941 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
    21001 cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg
    21061 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
    21121 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
    21181 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
    21241 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
    21301 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
    21361 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
    21421 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
    21481 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
    21541 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta
    21601 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
    21661 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
    21721 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
    21781 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
    21841 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
    21901 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
    21961 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
    22021 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
    22081 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
    22141 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
    22201 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
    22261 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
    22321 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
    22381 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
    22441 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
    22501 agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
    22561 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
    22621 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
    22681 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
    22741 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
    22801 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
    22861 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
    22921 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
    22981 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
    23041 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
    23101 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
    23161 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
    23221 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
    23281 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
    23341 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
    23401 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
    23461 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
    23521 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
    23581 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
    23641 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
    23701 aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc
    23761 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
    23821 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
    23881 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
    23941 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
    24001 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
    24061 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
    24121 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
    24181 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
    24241 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
    24301 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
    24361 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
    24421 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
    24481 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
    24541 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
    24601 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
    24661 gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
    24721 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
    24781 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
    24841 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
    24901 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
    24961 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
    25021 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
    25081 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
    25141 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
    25201 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
    25261 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
    25321 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
    25381 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
    25441 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
    25501 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
    25561 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
    25621 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
    25681 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
    25741 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
    25801 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
    25861 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
    25921 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
    25981 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
    26041 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
    26101 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
    26161 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
    26221 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
    26281 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
    26341 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
    26401 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
    26461 aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag
    26521 aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa
    26581 ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa
    26641 atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt
    26701 ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc
    26761 ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc
    26821 taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc
    26881 atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg
    26941 gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg
    27001 accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat
    27061 gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg
    27121 agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc
    27181 ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt
    27241 ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca
    27301 ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg
    27361 aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa
    27421 agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt
    27481 cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg
    27541 agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga
    27601 aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata
    27661 aagaatagga a
//
LOCUS       DACDBG010000030        26261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDBG010000030 DACDBG010000000
VERSION     DACDBG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.030"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(135..503)
                     /locus_tag="IVZ90_001308"
     CDS             complement(135..503)
                     /locus_tag="IVZ90_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8262372.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            653..1135
                     /locus_tag="IVZ90_001309"
     CDS             653..1135
                     /locus_tag="IVZ90_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8262373.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1172..2182
                     /locus_tag="IVZ90_001310"
     CDS             1172..2182
                     /locus_tag="IVZ90_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8262374.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2297..3010)
                     /locus_tag="IVZ90_001311"
     CDS             complement(2297..3010)
                     /locus_tag="IVZ90_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262375.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3200..3790)
                     /locus_tag="IVZ90_001312"
     CDS             complement(3200..3790)
                     /locus_tag="IVZ90_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8262376.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4274..4501
                     /locus_tag="IVZ90_001313"
     CDS             4274..4501
                     /locus_tag="IVZ90_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8262377.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4517..5683
                     /locus_tag="IVZ90_001314"
     CDS             4517..5683
                     /locus_tag="IVZ90_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8262378.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5917..6960
                     /gene="hrcA"
                     /locus_tag="IVZ90_001315"
     CDS             5917..6960
                     /gene="hrcA"
                     /locus_tag="IVZ90_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8262379.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7054..7617
                     /gene="grpE"
                     /locus_tag="IVZ90_001316"
     CDS             7054..7617
                     /gene="grpE"
                     /locus_tag="IVZ90_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8262380.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7668..9497
                     /gene="dnaK"
                     /locus_tag="IVZ90_001317"
     CDS             7668..9497
                     /gene="dnaK"
                     /locus_tag="IVZ90_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8262381.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9648..10814
                     /gene="dnaJ"
                     /locus_tag="IVZ90_001318"
     CDS             9648..10814
                     /gene="dnaJ"
                     /locus_tag="IVZ90_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8262382.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10896..11561)
                     /locus_tag="IVZ90_001319"
     CDS             complement(10896..11561)
                     /locus_tag="IVZ90_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8262383.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11537..11710
                     /locus_tag="IVZ90_001320"
     CDS             11537..11710
                     /locus_tag="IVZ90_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8262384.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11755..12492)
                     /locus_tag="IVZ90_001321"
     CDS             complement(11755..12492)
                     /locus_tag="IVZ90_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8262385.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12494..13996)
                     /locus_tag="IVZ90_001322"
     CDS             complement(12494..13996)
                     /locus_tag="IVZ90_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8262386.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14255..16249
                     /gene="uvrB"
                     /locus_tag="IVZ90_001323"
     CDS             14255..16249
                     /gene="uvrB"
                     /locus_tag="IVZ90_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8262387.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16259..19075
                     /gene="uvrA"
                     /locus_tag="IVZ90_001324"
     CDS             16259..19075
                     /gene="uvrA"
                     /locus_tag="IVZ90_001324"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8262388.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19338..20603
                     /locus_tag="IVZ90_001325"
     CDS             19338..20603
                     /locus_tag="IVZ90_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8262389.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20607..21338
                     /locus_tag="IVZ90_001326"
     CDS             20607..21338
                     /locus_tag="IVZ90_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8262390.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21473..22357
                     /gene="rapZ"
                     /locus_tag="IVZ90_001327"
     CDS             21473..22357
                     /gene="rapZ"
                     /locus_tag="IVZ90_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8262391.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22354..23352
                     /locus_tag="IVZ90_001328"
     CDS             22354..23352
                     /locus_tag="IVZ90_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8262392.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23386..24321
                     /gene="whiA"
                     /locus_tag="IVZ90_001329"
     CDS             23386..24321
                     /gene="whiA"
                     /locus_tag="IVZ90_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8262393.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24864..25514
                     /locus_tag="IVZ90_001330"
     CDS             24864..25514
                     /locus_tag="IVZ90_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262394.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25701..>26261
                     /locus_tag="IVZ90_001331"
     CDS             25701..>26261
                     /locus_tag="IVZ90_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8262395.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8667 a         4435 c         5586 g         7573 t
ORIGIN      
        1 ctccttataa aaatagcaga tactttgtca attaagtgtt taaccaggga tgataaaaag
       61 gataggattg aacaaaacct gtataaggtg atgaatagtt tcctcgccag taaaaggttt
      121 attcgtataa agctttatcg ccaaaattta tcatgacctt ttttatcttc aatattaaat
      181 gaaatcaatt gttcaatcaa ttcccagttg attacacttt tccaaggaac tttgatgacg
      241 ttatccgtat atacgtagcc tgcttcttgg atattttttt caagggcacg aatcgctgct
      301 ttttccggtg cgatagaaaa atgattttta gcaaaactaa atgcaataat aaaggtacca
      361 tggtcagtaa acataggttg gttccatttt acagttgtgt ctagctgggg atacgtttct
      421 gttatcttag caaaaaggtc tttgatcttt tgttgatgtt ctttttcttt gatttgattg
      481 atgatcggct gaaatttttc cattctaaaa cgcctccatt aagtggtttg atttatttta
      541 ccagaaaaac aatagttatg tataaaaaac gaacaaaaca caagtaatgt tgtaaccgct
      601 atatttatat gataagatgt ctcaaataat aaaatagaaa agagacataa aaatgattga
      661 cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc atcgagccat
      721 ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag gtacagagac
      781 caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga cttttactac
      841 gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt ctattgtttc
      901 atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc cgtttggtta
      961 tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa aacttgtttt
     1021 tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa acaaaatcat
     1081 ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca attaaccaag
     1141 taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat tagaaagaaa
     1201 tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc attcgttact
     1261 cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg gaaagacgct
     1321 ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg aattcaatca
     1381 aatgatgcaa gatgaaaata tccaaatgat ctttccgact tggggaggag aattattgat
     1441 tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag taggttattc
     1501 agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca ctgcacatgg
     1561 aactaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa tgtgggaaag
     1621 tgtactgaaa acaagagaag gagaatcgat tctacagcaa tcttctgaaa agtatcaaaa
     1681 agtgtggccc catgagcatt catcatcttc tgttttccat ctcactgaac caacaaattg
     1741 gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg gttgtattga
     1801 tacgatcagg catttaatcg gtactcctta tggagatgtc caatctttta aaagaaagta
     1861 taatgatgga aagccgatct tgtggtttct agaaaactgt gagctatcgt cagttgattt
     1921 aagaagatca cttgttcaga tgaaactggc aggctggttt gatggcagct cggggatcat
     1981 gttcggtaga agtgcggcag aggatttgtt agattataaa atgaaggatg tatacgaaga
     2041 tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc atgtaccgcc
     2101 acaactgaca ttgattaatg gtgcctatgc agaagtagcg gtagaagata gaaaagcgac
     2161 tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtctatc aaagaccaat
     2221 aaatctaaat agaaaagaaa gtgaaagaag ctgacaaaat gctgtcagct tctttttgta
     2281 taacatatta gaaagtctaa ttatctataa ctgtcacatc gattaactgt ttcacgtcat
     2341 tgggatttcc tgtaggaata gctactacag atgcattatc aatgatttct tgagttgttt
     2401 ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt gaaggaactg
     2461 gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc tggtaactgt
     2521 atttcccagt gagcataacc acatctttcg gatagactgc actccctgca tcataagggg
     2581 cgataggtgt tcctgcagga taatgaataa catgtaattc gaaatcaccc gatgtatctc
     2641 taaaagtatt acctctaata gctagccaaa cacgtgcgta ctcaatttgt agatcagaat
     2701 atcctgctaa cggatctatt gactgagaag actgtgagga ggttgtgtct attgttgaag
     2761 aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc tctgagactt
     2821 tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta cttgattttt
     2881 cagctgaact ggtttgtctg attgaacttt tgtctgtggt cgcagatgga tcactatttt
     2941 ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa ataaaaccta
     3001 actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata atcctgaatt
     3061 tttgaatccg taaaaccttg tctatgaata taaatagagt gtatcttctt tgataaatgt
     3121 cagaaaaagt gtgacaaata atcatcagta aaattacttg tcacactatt gataaggagt
     3181 atttaattga agagaatgat taggagaaag gatctccgta ctttttatag taatcataga
     3241 atccaagttt gtatagacga atcgtttttt ctcttatttc ttctgtttca aaatggactg
     3301 cttgaagaat caagtctgtg aattctatag gagctgttcc attcgcagta atcaaattac
     3361 gatctttcac agcttgttgt tctataaaat cttctttatt ctgataattt tccgattcta
     3421 accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg caataatccg ctctgagcta
     3481 aataatcaac tgccccacaa atagcagcta ctggtacgtc tgtttttaac aatttatcga
     3541 ttatttgata taaatgatga ttttttatgt cccatgaatt gccaccgatc ataataaaaa
     3601 ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat ttttgttttt aatcctccaa
     3661 tggaagtgac ctctgattgg atagaagcag ttttgttttg ccatttctca ttttggttta
     3721 gtgtgctagt aatataagca ccttcccaat cggcatattc ctctaataga aacgtaatcg
     3781 cagttttcat tttcaagctc tcctttacta tctataacaa ggacaacagt tttaacctac
     3841 agacattcat taaaattggg tagcgacagc gaaaacaaca attcttaagt tttagacaga
     3901 gtatacagat agtcaactta attaaatacg aactaaataa aaatgacagg taatcatttt
     3961 tgtttaccag cattataaca ttttgcatac atggatgaca aaagaaccaa ttcccacttg
     4021 cttgagtaca atattaaaag aaactggaag gcaaaaagta attatagaaa cgaaaaataa
     4081 atcatatcgg gcactcagac atgaaaacaa ccctagagat atataactag gtaaccagtt
     4141 taatgaaaga aaaaatcatc caagaagtcg attcttggat tttttagtgg atttgttcgc
     4201 caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg tctgagcact
     4261 taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact taatgccgaa
     4321 ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta gctccgatga
     4381 caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg cttatttcaa
     4441 tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact tttcgacgta
     4501 aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg tttctgcgta
     4561 cattcatatc ccattttgcg agcatatctg ctattactgt gattttaata aagttttttt
     4621 agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga aaataatgct
     4681 agaaagtcat ccgacagcga atttagaaac actttacgta gggggcggga caccaacttc
     4741 tttatctgct aaacaacttg atcgactgtt atcaggtgct agagagatcc tgccttttca
     4801 aaatggtaat gagtttactg tagaagcgaa tccaggtgat ttgacccgtg aaaaacttca
     4861 agtaatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat ttgataatcg
     4921 cttattgaaa aaaattggac gtaaacacac cgcagaagat gtgtatcaaa ctatgcaatt
     4981 tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat taccgggaca
     5041 aacactagaa ggatatctgg atactttgga aaaagctttg gctttggatc tgcctcacta
     5101 ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg tacgacaagg
     5161 aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga cgattgaagc
     5221 aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct ccggacatga
     5281 atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg gagctggtgc
     5341 tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac actatttacg
     5401 cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac gaaaaaatca
     5461 aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta aaaaacattt
     5521 ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa gggaattaga
     5581 gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa gagggacctt
     5641 tttggggaac aatgtgtttg agcgcttttt gtttgaaaaa tagtttaaag aatatttgtt
     5701 ttttatctga atttaaataa aaaaagttca aaaatacaat aaaggactat tgaaacgttg
     5761 atataactgg atttacatca attatttaga attagcactc ttgtgtttag agtgctaaaa
     5821 aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag tggattagca
     5881 cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg acaaaacgac
     5941 attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg ttccaaaaca
     6001 ttgatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat gaaagcatta
     6061 gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc atctgcttta
     6121 ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga agatgatttg
     6181 ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat tattaagcaa
     6241 tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg acctgaaata
     6301 aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca gattattgct
     6361 atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc cgaatatatg
     6421 ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt cggagatcct
     6481 ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca taaatatttc
     6541 caaacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt ttttgaagat
     6601 aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca agatcttgat
     6661 cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca aatgatcact
     6721 ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga tttattgcag
     6781 aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg tacaatagcc
     6841 ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga tgtttatcga
     6901 agagaactag cagctaaact tgctgattac taccgttcgc ttgacttgtc aaactcgtaa
     6961 tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag agcaatcaaa
     7021 cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga agaactagat
     7081 aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc agaagattcg
     7141 ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc tgagttagaa
     7201 gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc aaatcgagga
     7261 aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa aaaattactt
     7321 ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga tcaaggggca
     7381 ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact ggaagaagaa
     7441 ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca tcaagcagta
     7501 caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt actacaagaa
     7561 ggctacaaat tacatgatcg cgtgttaaga ccaaccatgg tcattgttgc acaataattg
     7621 tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg agtaaaatta
     7681 tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga aacgaagcaa
     7741 aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca ttcaaaaatg
     7801 gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca aatacgattt
     7861 catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa ggtaaatcat
     7921 atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc gcagaagact
     7981 atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt aatgatgcac
     8041 aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa cgtatcgtca
     8101 atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc gacgaaaaaa
     8161 tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa ctaggagacg
     8221 gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac gactttgata
     8281 acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt gacttgtctc
     8341 aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa aaagatttat
     8401 ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa tctggaccat
     8461 tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct gacttagtag
     8521 aacgtacaaa agtaccagta cgtcaagcgc taaaagatgc aggtttatca caatcagaaa
     8581 tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta gaagcagtac
     8641 gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta gttgctatgg
     8701 gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt ttacttgacg
     8761 ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa ttgattgacc
     8821 gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca gataatcaac
     8881 cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac aacaaaactc
     8941 taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca caaatcgaag
     9001 taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat ctaggtacac
     9061 aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa gaaatcgaac
     9121 gtatggtaaa agatgcagaa gcaaatgcag aagcagataa agctcgtaaa gaggaagttg
     9181 acctacgtaa tgatgtagat gcattgctat tctctgtgga caaaacattg aaagaattag
     9241 aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgcgat gaattgaaag
     9301 ccgctgttga agcaaacaac atcgaagaaa tgaaaactaa acgcgatgca ttgaacgaaa
     9361 tcgttcaaaa cttgactgtt aaactatacg aacaagcggc acaacaacaa gctcaagaaa
     9421 acccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt gaagaagtcg
     9481 atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt tgctactttg
     9541 gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa aatatgatat
     9601 gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg gcaacaaaac
     9661 gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa ataaagaaag
     9721 cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct gatgcggaag
     9781 aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa aaacgcgctg
     9841 catatgacca atacgggcat gcaggcacag atccaaatta cggtgctggt ggagctggtg
     9901 gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt gaagatatct
     9961 tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct cgtcaaggtg
    10021 ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt gtagaaaaag
    10081 aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tgggaacggt gcaaaaccag
    10141 gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgattaat gtagaacgcc
    10201 aaacaccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat gggacaggga
    10261 aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag aaagcacatt
    10321 cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt ttagctggtc
    10381 aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc ttccgtgtag
    10441 aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg cctttaagtt
    10501 ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga gatgtgaaat
    10561 tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga aaaggcgctc
    10621 cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg atcacaccga
    10681 aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct ggcggcatca
    10741 aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct tttggcggga
    10801 aaaagcggaa gtagtttttc tagtaaaaac gctaagtcat taataattaa aaaccagacg
    10861 aacaatcata tgttcgcctg gtttttttgt agttgtcatg tttgtaccaa caaaaaagcc
    10921 agctttagaa tatgataatc gtcatagttt ctaggggtat aaccagtgag agaatggaac
    10981 ttgttcaatc gatattgcaa ggtgttttta tgaataaata attcttctga gcaatttttg
    11041 atgcttccgt tatttttact gaagcatgcc aaagtcttct tgaacaaatg tacttcttct
    11101 tctgtcaggc ttttcaaaat acgttttaca tataatgttc gttgatctga cgggattgct
    11161 ggtaaaacga tcccgaggtc taaatcttca aaaaagcgga tctcgttcac agattgatgg
    11221 aaagctatcc attcgcaaac ttgtttggca tgaaggaaag attgcggtgt ctcttgctct
    11281 gtataacaaa gcgacccaat tccaaagttt agctgtaact gatacatttt attaaacttt
    11341 gtctggattt tatcaagcaa tgatagcaat gaggctctgg aaatccgatt gactaggata
    11401 atgatttgtt gtctatgtac agtgagcaaa gcctgtccgt aataagagaa gaccctttcg
    11461 agcaactcca ataaaggatt gatccgttct tgacacaatt gctgattgtc agaaagttgt
    11521 cccacaacta cataatatgg agaagaaaaa tcaagctcaa ttaagtgagg gtggtatcga
    11581 ctatgtactt gatatcatca atgaaccgat ggtactttct tatgcgatgg aacaggtaga
    11641 gcgattgctt gtttgtgcag gaaaacaagc gatgcggctg atacgattag gtgagaaaat
    11701 aagaagataa aaatacacgg tcccttcatg gcgaccgtgc atttttggtt tttattaaat
    11761 atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt caaagatttg
    11821 ttgaggcgtt ccatcttcta aaaagtttcc gtcgtcaatg aacatgacgc gattggccac
    11881 ttctttagcg aaccccattt cgtgcgtaac gataaccatc gtcatacctt gttctgctaa
    11941 ctttttcatg acgttcaaga catcaccgac catttcagga tcgagtgcag aagtaggctc
    12001 atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca cacgctgttt
    12061 ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac caactgtctg
    12121 cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca ggtccatcgg
    12181 tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt gttggaatac
    12241 cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca agtgggcgcc
    12301 gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac gcaagaatgt
    12361 actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt tgatggatac
    12421 attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct caactaagat
    12481 tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt gtcaatgctg
    12541 tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt tgtgttgtac
    12601 gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg gaaatgatcg
    12661 ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg atgcgtatcg
    12721 cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa cttctggaag
    12781 cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct gcgatataag
    12841 cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc gtaaatccaa
    12901 cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga attcctcgga
    12961 tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct acactgaata
    13021 atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt aggagaattg
    13081 ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca agaaatgttg
    13141 attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca tattctttaa
    13201 taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg aacatattaa
    13261 gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca gattcttttt
    13321 tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc ggataatcat
    13381 ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca gcagcatcaa
    13441 aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca gcactttcgg
    13501 taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg atcgaagtat
    13561 ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa aaatcatagc
    13621 ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct tgtcctgatt
    13681 ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta aatccttgca
    13741 gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc tttgcgtttt
    13801 gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct tttttgggct
    13861 ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg tcagcttgtg
    13921 caacatgacc aaacgcaaat gtcacaagtc ctgtcattaa gaaaacaaat gagtgaaata
    13981 aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt ataggcgaaa
    14041 agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga gtttctaaca
    14101 ttttatgtta taaaacataa catgtataat aagataaaaa agtggcgaaa gtatgttcgg
    14161 tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta ttggtcgtaa
    14221 aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata cctcccgaca
    14281 ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga ttaaccaatt
    14341 agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga ctggaacagg
    14401 gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac tgattattgc
    14461 ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt tccctaacaa
    14521 tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt atgtgccttc
    14581 aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca aattacgaca
    14641 ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat ccgtttcatg
    14701 tatttttgga ttaggctcgc cttttgagta ccaaaaacaa gtagtttcga ttcgacaggg
    14761 agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat tcgaaagaaa
    14821 tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg agatttttcc
    14881 ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg tcgaacgtat
    14941 ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg tagcaatctt
    15001 cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag cgaatatcaa
    15061 agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc tggaagctca
    15121 acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg gctacacgtc
    15181 gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac cgccttatac
    15241 gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc acgtaaccat
    15301 gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc tagtagatta
    15361 cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag agtttgaaaa
    15421 acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac atgaacaaac
    15481 agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag tgatcgaagt
    15541 tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac gagtcgaaaa
    15601 agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc tcacagatta
    15661 tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa cgctggaacg
    15721 aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg gaatcaattt
    15781 gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg atgcggataa
    15841 agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag ctgctcggaa
    15901 tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc gtctagcgat
    15961 ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac atgggatcgt
    16021 tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag aatcggaaga
    16081 tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag aaaaagacac
    16141 gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg attttgaaac
    16201 agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg aggacaacat
    16261 ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa atatagatgt
    16321 aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg gaaaaagttc
    16381 cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga gcctttctgc
    16441 ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca tcgacggatt
    16501 gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt cgacagtagg
    16561 tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag gacatccgat
    16621 ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg cagatcaagt
    16681 aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg taaagaaaaa
    16741 aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc gcgttcgagt
    16801 cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca aaaaacatga
    16861 tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc gcctatttga
    16921 ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg tgatcggtga
    16981 agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta ctgttggcga
    17041 actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag actgtgatgg
    17101 tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa cgaagacact
    17161 tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc ctcaaatgtt
    17221 agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag aattaccgga
    17281 agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc attatgaaaa
    17341 tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga agaatatcga
    17401 gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc tttatatgac
    17461 agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc tatctgttca
    17521 aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag cagtagattt
    17581 cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga ttttaaaaga
    17641 agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa ctttgagtcg
    17701 cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta cacaaattgg
    17761 atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat tgcatcaacg
    17821 cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca acaccctcgt
    17881 tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg acgtcggacc
    17941 tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag aagtagctaa
    18001 tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc ctgtaccaaa
    18061 agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag aaaacaatct
    18121 gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag gtgtgtctgg
    18181 ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc aaaaattaaa
    18241 taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa gtatcgaaaa
    18301 gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc cagcgactta
    18361 tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg caaaaatgcg
    18421 gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg aagcttgtcg
    18481 cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg ttccttgtga
    18541 agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca aaggaaaaag
    18601 cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca agcacattcc
    18661 aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg tgacgatggg
    18721 gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg ccagtgaatt
    18781 gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga caggacttca
    18841 tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg ctggtaacac
    18901 agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata tcattgattt
    18961 aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac cagaagaaat
    19021 cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg tataaagagg
    19081 atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg ttttattaat
    19141 atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta tagataaaaa
    19201 cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact ttcctttgat
    19261 tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa caagagatta
    19321 tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc cctatattat
    19381 taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca tacggcaaaa
    19441 tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa attgtcaatg
    19501 tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg cttcgtttgg
    19561 caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt ggtgatggat
    19621 atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc tgtccatata
    19681 ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa gtttgtgaag
    19741 aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta gtaaatggac
    19801 atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca aacagtgtgg
    19861 agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag actagagaag
    19921 agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa atgattgtac
    19981 aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat gtagatgaag
    20041 atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct acaccagcat
    20101 cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat caaaccatta
    20161 aggcattttt ggaaaaaatc gaatatacag gctttgccaa tttcgatatg aagtatgatc
    20221 ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt agcagtttct
    20281 ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga gtgttcaaca
    20341 aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga ttatggatgg
    20401 gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa aaactagctg
    20461 agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa gaccgatcaa
    20521 tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga ttcaaaaagt
    20581 attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta ggcggaatgg
    20641 gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa gctacaaaag
    20701 atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat cgtacagctt
    20761 atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat attgagaaac
    20821 aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac tacttttttg
    20881 aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa gcagcaaatg
    20941 aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa ggaagtatga
    21001 aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg attcctgata
    21061 ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa tcggattatt
    21121 tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta aactgtgaaa
    21181 aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa gataatcatt
    21241 acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt gcattagctg
    21301 aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt tggggcaaat
    21361 atcctaaaag atatttggtc caaatctttt tgtaaaagaa aaagaaaact ggtaatgata
    21421 cgcccgggat gatataattt tattatgcac aatgtgagag gggttcgtaa atatgacaga
    21481 taatttgcaa ttagtcatca taacaggtat gagtggtgca gggaaaacag tagctatcca
    21541 aagttttgaa gatatgggct atttttgtat cgataatatg ccgcctcgat tgattccaaa
    21601 attctgggaa ttgatcaaag aatcaggtaa agtcacaaaa atggctttag gagtggattt
    21661 gcgttctcgt tccttctttg aagaaatcca aaacatgctg atcgaaatcg aaaatacgaa
    21721 cttcatcgac acttctatct tatttttaga tgcttcagac gaagaactag tggctcgcta
    21781 caaagaaaca agacgagcac atccaatggc gatggacggt ttgatcactg aagggatacg
    21841 taaagaacgg gcgatactag acgacttgaa agcccaagct tccgtgatca ttgatacgac
    21901 aacattgaca ccacgacaat tacgtgaaaa aatcaatgag tcattcaaat caaagggaga
    21961 ccaaggattt cggatcgaat ttgtatcatt tggttttaaa tacgggttgc cgatcgattc
    22021 ggatattgtg atggatgtgc gttttttgcc caaccctcat tatatcccgg aactaagacc
    22081 attgaccgga aaagatcagc cagtctatga ttatgtgatg tcctttgatg aaacgcaatc
    22141 attctaccat aaatttttgg atcttctgga aactgtattg cctggataca ttaaagaagg
    22201 aaaaagcagc ttgacagttg caatcggctg tacaggcggt caacaccgtt ctgtggcatt
    22261 gactgaacgt ttggcaaatg ctttagggaa aaactataaa gtcaatgtga cacatcggga
    22321 caaggataaa aggaaagaga cggtgaaccg gtcgtgaaaa tgaaaaccta ccggataaga
    22381 aaaccaaaga tcgtagtggt cggcggtggc actggtctgc cagttatcct gaaaagttta
    22441 cgaaatcaag gagcagatat cacagcagta gtgactgttg cagatgatgg tggaagcagc
    22501 ggtgcaatca gagaatcgat cgctatggct cctcctggtg acttgagaaa tgtcttagtg
    22561 gcgttatcgg atatgccaca attctatgaa gatattttcc aataccgttt caaaaaggaa
    22621 gatcagtttt tagccaatca tacgatcggt aatctgatca ttgccgcagt ttccgagatg
    22681 cggggaagca cttatgaggc gattcaatta ttagcaaaaa tgatgcatgt agatggacat
    22741 gtttatcctt cttcagaaac tcctttgacc ttgcatgctg tttttaaaga cggaacgact
    22801 gcagtaggag aatctaaaat cgcgatcaac cgtaaaacca ttgaccgtgt gtatgtctgc
    22861 aacacaaatg ataaaagtga agccaaagcg gctcgaaaag tcgtttcagc aattatggac
    22921 gccgacatgg tcgttttggg acccggaagt ttattcacca gtatccttcc aaatttagtg
    22981 ataccagaaa tcggcgaagc aatgctcaag acaactgctg aaaccgtcta tatctgtaat
    23041 atcatgaccc aaaaagggga aacggaacat ttcacagatg ccgatcatat acgtgtttta
    23101 cacaagcatt tgaatcagcc ttttatcgat acagtattgg tcaatacgga aaaagtgcct
    23161 gaagattata tggacccaga gatctatgat gagtatctag tacaagtcgc acacgacttc
    23221 aacggattga gaaatgaagg atgtcgagtg atttccaccg actttttaga attgaaagat
    23281 ggtggtgtct tccacgatgg agacaaagta gtagaagaat tgttccgttt agttttcggt
    23341 tcaaaatatt aagcaattta ttgttggaga aaggaggaga ataggatgtc atttgctgca
    23401 gatgtgaaga aagaactaac tggtttggaa gttcatcgag aacatgcgaa agcggaatta
    23461 gctgctttgc tgcgaatgaa cggctcttta ggtattgtga accagcagtt tattttgaac
    23521 gtccaaacag aaaatgctgc aattgccaga cgtatctatt ctcttttgaa agatcattat
    23581 caggtacgaa gtgagctact cgttcgtaaa aaaatgaaat taaaaaagaa caatgtctat
    23641 atcgtccggt tgaagcaagg cacaaaagat gtgttgatgg atcttgatat catggatggt
    23701 atgatgttca atggacacgt ttcggacgag attatgggaa attcgcaaaa aatgcgttct
    23761 tacttacgtg gtgcttttat ggcctcaggt tcagtcaata atccggaaac gagtcgttat
    23821 catttggaaa tattttctat ttatgaacag cataaccaag acatttgcga tatgttaaat
    23881 tattacggtc tacatgctcg aacgcttgaa cggcgcaatg gctatatttc gtatttaaaa
    23941 ggagcagaaa aaattgctga tttcttaaca ttgattggtg caaccaactc aatgctccga
    24001 tttgaagatg ttcgaatcgt acgggatatg agaaattcgg tcaatcgtct agtcaattgt
    24061 gagacagcaa atctgaacaa gacgattgat gcggcttcta aacaaatcga aaacatcgaa
    24121 ttgatcgaag caaaggtcgg tctacatgcc cttcctgaaa aacttcagga aatcgcagaa
    24181 cttcggcttc aacatccaga ggtcagcttg aaagaattag gggaaatgat tccctcaggt
    24241 gcaatatcaa aatcgggaat taatcaccga attcgcaaaa taaatgaatt tgcggaaaaa
    24301 ctgcgtgaag aagcagttta atccaaagaa aaaactgata catgaagcag gagatgcgaa
    24361 tgtatcagtt ttttttaaaa gctatacttg cttttcttcc gtcaaactat aattccaact
    24421 ttactggagt aacataatgt tgaaaggctt ctattgtttt ttaatacaat cgtataaaaa
    24481 gcggggagac aactgaatga agattttagt tttatacata aacttttgct taatatagaa
    24541 gtcctgatat agataggatc aagtatttat ctataactgg agattaatta aaaactggaa
    24601 ggaactgctc gatcaacctt gagaactgtt tttcttactt ctggtcaaga ttctcgtggt
    24661 tggtggataa ggatggttga taagttcatt ggattagcta gtcgaaaaca accaggtgta
    24721 attaatgtag aagttttaac aaggggatgg agtgatttat tcagtgataa tcataagttc
    24781 ataaatagta attgggatgt atggttggta tacgttatac ctgaacttga actatcaaga
    24841 tttggtttag aatttgagaa tcgatgttta taaagggagg gcaaaaaatg tgctttttta
    24901 caacttttac tatcattatg attgcagtac tttttatttt tctagatata gcaaagcgaa
    24961 atacagcatt tttattatat cgggttttac ttcgagcagg tctcataaca tttatatcga
    25021 tcgttggatt ttttctattc acagttattg tatttatttg gagaactaca gcaccaccat
    25081 tgccagagat tacatatgga gaatttccgt ttagtcttga atatgaaata gatgaggaaa
    25141 gaattttgat tgaagatacg gtgattgcag aatttatagg aagttttaga gggaatgtga
    25201 caactcaacc tagacgaagt tggagtataa aacttttaaa taatagtgga gagttgaaag
    25261 catttcatta tgaattctta ataatagaaa gtgaagatgt aacaatcact ttaagtagtg
    25321 gctctgctgg atattttatg aatgatggtg gaagcaagcc acagctcaca gtccgtgatc
    25381 gtattgcagg ttttatgcat ttttctcact atgacttaga agaagctcgt aaaatacttg
    25441 cagaatatgg gatcacatta atcaattggg aatatgcgcc accgataaga aattttttca
    25501 atgattatta ttagtcttat aaaaaacaga atgtaaaatt tatgtgcgcg agtatttagt
    25561 tttccatttt ttgtttgata gatatatagc attgttgaag catgggatag tggtgccaat
    25621 tttatcaatg aggttggtca ggcagttaac ataattggtg atgctataaa tccatttaat
    25681 tggaattgga ggaattaggt gtggaaacag tgagattatg gatattcatg aaaaattttc
    25741 gttcggcttt gagatatgtt attctggaat caaaatcagg ttattatctg attgacacag
    25801 atcactatac tttttgggga ggctaccttt ttccgatttt taattggata atcccccata
    25861 aggttatgaa aattaacttg cctgcagaag aaataaatag attaatactc aacaaagatt
    25921 tacaaaggaa aagagagaaa gaacattcta atgttgcttg gataggtcca gcatttgcag
    25981 tagtgacttc ttcaattgtc ggtcctatgc tagagagtat ttatagtgat ttttctattg
    26041 gaatacgtta tttatttgtt actttaagta tagctgttct tttgtgcata aagggttatg
    26101 ttagcaagaa gaataaagat attttaacac taatcggtgt aaacaattta tctagtacta
    26161 aaattatact gtttcctgaa tctataaaag aaggatcaag gttgttgata atttttataa
    26221 tgcttatact gttttctgtt ttatttgggg tgatgtttat a
//
LOCUS       DACDBG010000031        25501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDBG010000031 DACDBG010000000
VERSION     DACDBG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.031"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IVZ90_001332"
     CDS             132..3659
                     /locus_tag="IVZ90_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8262396.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IVZ90_001333"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IVZ90_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8262397.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IVZ90_001334"
     CDS             7447..7788
                     /locus_tag="IVZ90_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262398.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IVZ90_001335"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IVZ90_001335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8262399.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IVZ90_001336"
     CDS             9145..11565
                     /locus_tag="IVZ90_001336"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8262400.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IVZ90_001337"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IVZ90_001337"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8262401.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IVZ90_001338"
     CDS             12999..13736
                     /locus_tag="IVZ90_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8262402.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IVZ90_001339"
     CDS             13750..14565
                     /locus_tag="IVZ90_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8262403.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IVZ90_001340"
     CDS             14578..15225
                     /locus_tag="IVZ90_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8262404.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IVZ90_001341"
     CDS             15241..15897
                     /locus_tag="IVZ90_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8262405.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IVZ90_001342"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IVZ90_001342"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8262406.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IVZ90_001343"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IVZ90_001343"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8262407.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IVZ90_001344"
     CDS             18239..18757
                     /locus_tag="IVZ90_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8262408.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IVZ90_001345"
     CDS             18864..19364
                     /locus_tag="IVZ90_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8262409.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="IVZ90_001346"
     CDS             complement(19644..20564)
                     /locus_tag="IVZ90_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8262410.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="IVZ90_001347"
     CDS             20729..21742
                     /locus_tag="IVZ90_001347"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8262411.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IVZ90_001348"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IVZ90_001348"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8262412.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22827..23267
                     /gene="fabZ"
                     /locus_tag="IVZ90_001349"
     CDS             22827..23267
                     /gene="fabZ"
                     /locus_tag="IVZ90_001349"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8262413.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23382..24143)
                     /locus_tag="IVZ90_001350"
     CDS             complement(23382..24143)
                     /locus_tag="IVZ90_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8262414.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24332..25426
                     /locus_tag="IVZ90_001351"
     CDS             24332..25426
                     /locus_tag="IVZ90_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8262415.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7245 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttgatttctt aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa
    22741 aaaaagattg ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc
    22801 aattgcctga aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg
    22861 aattgattcc taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag
    22921 gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact
    22981 ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt
    23041 cgatacttat cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca
    23101 ataaggccaa attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa
    23161 tcaccaaaat gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa
    23221 aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga
    23281 tatagcaaaa caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct
    23341 agcatacgtt gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct
    23401 tgcagtatat tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt
    23461 tcgatatttg caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta
    23521 taatggccat ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga
    23581 tagtcgtctt ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg
    23641 ctgccgttac tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa
    23701 aggtggttgt tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca
    23761 tcgcctttag gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt
    23821 ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt
    23881 tcgggaaata atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca
    23941 ttttcggcga catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg
    24001 gcagtcgtgc tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa
    24061 gtcgtgttgt ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt
    24121 aaaataactt tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta
    24181 atgataacaa aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta
    24241 aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca
    24301 aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc
    24361 agaacggtca acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta
    24421 taaacgggtt tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga
    24481 atttaaagaa gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa
    24541 tccagcgtta tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat
    24601 gggtgcctac ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac
    24661 gcgcaatcct tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga
    24721 caacatcatg agttattcgc caaacacgga taatttatta ggttctcata atttttatcc
    24781 tcatcgatac tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa
    24841 atataatttg aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc
    24901 tacgcaagat ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt
    24961 gaagcattta atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag
    25021 tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt
    25081 agatacggaa gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag
    25141 ttatcgaggg gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa
    25201 agataaagat ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga
    25261 caatgaaggg tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa
    25321 cgacggacgt gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt
    25381 tttgaaacca tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg
    25441 cagaaataat ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag
    25501 g
//
LOCUS       DACDBG010000032        25494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDBG010000032 DACDBG010000000
VERSION     DACDBG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.032"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(169..3318)
                     /locus_tag="IVZ90_001352"
     CDS             complement(169..3318)
                     /locus_tag="IVZ90_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8262416.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3332..4549)
                     /locus_tag="IVZ90_001353"
     CDS             complement(3332..4549)
                     /locus_tag="IVZ90_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8262417.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4539..6134)
                     /locus_tag="IVZ90_001354"
     CDS             complement(4539..6134)
                     /locus_tag="IVZ90_001354"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8262418.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6259..7044
                     /locus_tag="IVZ90_001355"
     CDS             6259..7044
                     /locus_tag="IVZ90_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8262419.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7753..8112
                     /locus_tag="IVZ90_001356"
                     /pseudo
     CDS             7753..8112
                     /locus_tag="IVZ90_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8407..9540)
                     /locus_tag="IVZ90_001357"
     CDS             complement(8407..9540)
                     /locus_tag="IVZ90_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8262420.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(9531..10199)
                     /locus_tag="IVZ90_001358"
     CDS             complement(9531..10199)
                     /locus_tag="IVZ90_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262421.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(10655..11641)
                     /gene="lacD"
                     /locus_tag="IVZ90_001359"
     CDS             complement(10655..11641)
                     /gene="lacD"
                     /locus_tag="IVZ90_001359"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8262422.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(11654..12580)
                     /gene="lacC"
                     /locus_tag="IVZ90_001360"
     CDS             complement(11654..12580)
                     /gene="lacC"
                     /locus_tag="IVZ90_001360"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8262423.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(12593..13108)
                     /gene="lacB"
                     /locus_tag="IVZ90_001361"
     CDS             complement(12593..13108)
                     /gene="lacB"
                     /locus_tag="IVZ90_001361"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8262424.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(13127..13555)
                     /gene="lacA"
                     /locus_tag="IVZ90_001362"
     CDS             complement(13127..13555)
                     /gene="lacA"
                     /locus_tag="IVZ90_001362"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8262425.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(13575..14348)
                     /locus_tag="IVZ90_001363"
     CDS             complement(13575..14348)
                     /locus_tag="IVZ90_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8262426.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            14513..14992
                     /locus_tag="IVZ90_001364"
     CDS             14513..14992
                     /locus_tag="IVZ90_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8262427.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            15049..15351
                     /locus_tag="IVZ90_001365"
     CDS             15049..15351
                     /locus_tag="IVZ90_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8262428.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            15373..16830
                     /locus_tag="IVZ90_001366"
     CDS             15373..16830
                     /locus_tag="IVZ90_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8262429.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(17423..18175)
                     /locus_tag="IVZ90_001367"
     CDS             complement(17423..18175)
                     /locus_tag="IVZ90_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8262430.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(18390..18626)
                     /locus_tag="IVZ90_001368"
                     /pseudo
     CDS             complement(18390..18626)
                     /locus_tag="IVZ90_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(18636..19594)
                     /locus_tag="IVZ90_001369"
                     /pseudo
     CDS             complement(18636..19594)
                     /locus_tag="IVZ90_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(19800..21482)
                     /locus_tag="IVZ90_001370"
     CDS             complement(19800..21482)
                     /locus_tag="IVZ90_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8262431.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(21493..21816)
                     /locus_tag="IVZ90_001371"
     CDS             complement(21493..21816)
                     /locus_tag="IVZ90_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8262432.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(21839..23245)
                     /gene="lacG"
                     /locus_tag="IVZ90_001372"
     CDS             complement(21839..23245)
                     /gene="lacG"
                     /locus_tag="IVZ90_001372"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8262433.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            23712..24719
                     /locus_tag="IVZ90_001373"
     CDS             23712..24719
                     /locus_tag="IVZ90_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8262434.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(25113..>25494)
                     /locus_tag="IVZ90_001374"
     CDS             complement(25113..>25494)
                     /locus_tag="IVZ90_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8262435.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         7605 a         5313 c         4210 g         8366 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctgtctt
       61 aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc
      121 cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc
      181 attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg
      241 tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact
      301 gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga
      361 agcatgtaat tctttctcag aaacaaacca ctcattggaa aaggcatgaa tcaaattatc
      421 acttgcttgt gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt
      481 ccccacatca aatgcattta aaatttgtcg gtaaacttct ttgactcctt ctgttttatt
      541 caatttagcc aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt
      601 tcctagtaat tgatcaatat aagtagaatc aatatcatac aacttagtgg tattttcact
      661 ataaaattcg atcgttgaaa aatcttctcc ctctttttct ggttcttcct ccatcaatga
      721 acccttcacg gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa
      781 agttgaagat tgcaccatgt catcattgta atcatcataa gtcactaacg attcatacgc
      841 acgattcaat tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc
      901 atcaatggat tccatggatg aaacctcttt taacttacca tatgcttgct tgaatctttt
      961 tttggattct tggtaacttg gataaatcaa acccgattct tctttagctt ctgaatacag
     1021 ttttgtcgca tcctcgacat tcttctccat tgtcgctggc tttcggaaag taacaattag
     1081 tccttttgtt ttttcaggat acgtacggtt cgtacgtgaa aaggcttgga tcaatcctgc
     1141 atactctaga ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgtcaa
     1201 tagacgatcc actacgatta ctaaatcgat ttgtttccca aattgtttga attccgctct
     1261 ttttcgtgcc aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctagtgacca
     1321 ggcagtatca taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc
     1381 acttgaatct cggctatttt cttctaatga ataggtaata gcgattcgtg ggaaatcagg
     1441 atcctccatc gttcgtcctt cacggatagg ctgattggga aactctttta ttaaccaatc
     1501 agattctttc gtcatctctt tgatggcccg ataatacttt ttggccatat caatggaact
     1561 cgtggttaag atcgcagact tcgttggacg gccatttcga aaatcaaatt ttgtataggc
     1621 attgtctgga cggaacattt tatgaatgac tttttggata tgttcctctg cttcaaaaac
     1681 agtagggtcc aaataacttt cttttttgac agactccatt tgatcgatca tacgattgat
     1741 ctgttccggt gaatactctg catatgtttc tacttctttt aatttccgat agattttatt
     1801 ctctaatgaa gtcggctcca cggtgttttc atgctctact tgaaatccta aaaccgagcc
     1861 atcttctaat gcattcttga tggtataggt gtgcaagacc tctccatact gatcatgggt
     1921 cgtacgtgcc aattgccctt tggcttgttt ccgattctca gggaaaatcg gtgtacctgt
     1981 aaagccaaac caagtggatt tcggaaacat ttttttgatt tcttccatat tttcagcact
     2041 taacgctcga tgacactcat caactacaaa cacgatatgt tgccccatta atttttggaa
     2101 gcgattggtt ccttttttct cttcctgttt ctttgcatat ttcaacgccg catctagttt
     2161 ttgacgggtt gtaatgatca caacattcgc attggcatct gccaacagag tttcacttaa
     2221 ctctttggca cttcctgttc ccacgatcaa gctattcgct ttggcattac cagaagaaac
     2281 cccagtatta aactccgaag caaatttcgt aaactctgtg gtggtctgat tatctaaatc
     2341 tttgcggtcc actaacatga tcgttcgatc gatccccgat tttcgtgcta aaagcttagt
     2401 agatacaaaa ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg
     2461 atgtttattt gcggcaataa acaaggcttg aatcgcatgc acttgataag gatgtaacac
     2521 catgagggtt ttattgtctt gatcttcact gacaatcgta taatccgcaa tcaagcgatg
     2581 ggcatctggg atatttagca cttgtttgca aaactcatat aagttttcta cctttttatt
     2641 atctttcgtt cgccaactaa ataaaaactt aggatgcaaa tccttcggta aggcatttgc
     2701 aaaataccga gtcgtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt
     2761 gttccgaaac atgccttctt cagcatattt tttgatttga ttaaacgcct gataaaaacc
     2821 atcttttgca gttacttgtt ttagctcgat ttggataatc ggtaagccat tgatcaataa
     2881 ggtgacgtca aaacgtcgat cccgcgcttc tatgttactt ccacgttttg caatttgatg
     2941 gaccacttca taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga
     3001 gacagaaccc aattgcggat tttcccgttc aatcgtgatc cgagccatcc cattttcacc
     3061 ttttaaccat ttcgcagcat caaatggggt tttcgtccgt agcaataatt cggttttgat
     3121 cgtttcaaac tctttatcgg tcaaaggaga atcattcagt tctgcttgat tatttgagat
     3181 gattttttga cgtaaattgc tccaaagatc ttcttctgat tttagatccg gacgatacgt
     3241 ccactgatta tgtccttccc cgaggacacg aatcaactgt tcttcgactt ccgcttcctc
     3301 acggtgacta attttcattc ttcttccctc cctaaacaaa catcttttgc aagaagcctt
     3361 ttttcgtttc cttcagcaaa tctaacttac gttgatgaag agcgatcgta tcatctagct
     3421 gcttgaagaa ggcaccaatt ttttgttgtt catttatatc cggtggaaac tgaaatgtcc
     3481 tttgttctat accaccttta gtcacatgta ccattgtcgt tccgttagtt gattctttaa
     3541 ttttctcttt atcagtcaat agccaagtat atagatactg tttgtccaat tccttagata
     3601 aaaatttaag tttccaaata tgataatggt agattacacg ttcttcattc cacatttcag
     3661 gaccgaagct tgttgcccat aaatataaga gatctccttt attagcatac ttgtcttcct
     3721 ctaattctaa gtttgaataa taccatctat cattagtatt aaaattacct actcttaaaa
     3781 ccctatattt tccagaatca agtaattctt gttgtttaaa agctctacca ttcaaaaaaa
     3841 ctaccacttc ccccaacttc cgctcttccc aatctcccgt aaatcctgga aaacgaactt
     3901 ctggcacttt tgctccgttt ttagggaaca ttttttgtaa gaatcctttt ttcgtttcct
     3961 tcagcaaatc taattcgtgc tggtgaagca tgatggtgtg atcaagttgt ttgaaaaatg
     4021 aaccgatctt gatctgttcg tcataagttg gaagtgagat aggtgtcttt ttaccactat
     4081 caattgtata ggttccgacg gttccaatgc ctgcaacgtc acggatatac ttctgaattt
     4141 tgttatttat gaacgagtag taaaagaagt ttccgtcaat tttatgctca ttcttgaacc
     4201 aaatgatgtt tccatcctta aagtaaattg ggttatcatc agtgattaat aaggggacac
     4261 ctattgatcc gacaccagtt acaagcaagt ctccttgaga aactttacct gaatttttcg
     4321 aatattcatt atatttttct tctgaaatgt aaagataatc ggatggttcc tcattttttg
     4381 cagctgagac aatatcacga gcacgtaaaa atctaatacc tgagttagtc caatctgatt
     4441 gatgaattcg cttcacagaa gtaacatcca tcatatctcc taacttacgc tcttcccaat
     4501 cttccgtaaa accaggaaac cggatttctg gtcgtgtatc attgctcatg tttgaacacc
     4561 tccagcgcac cttgtaacca cgcttcattt tcaggtgttg tttgcaggga agagatagct
     4621 tctaaaagtt ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg
     4681 acatgaacca tatcaattgg ttcttcttct tcgaaagtat cgacatagcg tgggatattt
     4741 aagttatagt cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca
     4801 tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga taatttattc
     4861 tgatttttcc ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga
     4921 cgattcttct tcaatacaat caccgtcgtt ggaatcgatg taccaaagaa caagttcgca
     4981 ggcataccga tcacagcata aatactgccg tcttctaata atttttgacg aatgacccct
     5041 tctgcagcac cacggaataa aaccccgtgt ggcaagacga tagccatagt ccctgtttcc
     5101 ttcaaatgat agaaaccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat
     5161 ttcccgtagc gattaaaccg agaatcatct aagaaggtcg tatctgctga ccaattagcc
     5221 gaataaggtg gattcatcac tactgcatca aatgtgtaag gttcatctgt tggccagtcc
     5281 ttattcaacg tatccccatt gcggagattc atttcttcgg catctacccc atgtaaaatc
     5341 aagttcattt ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg
     5401 ttatctggat gagttaagta gttacgtaca tttaacatca aggaacctga acccatggtt
     5461 gggtcaaaga cactaaagaa tcgtcgttct ttttgatcca acgtcacgat ttgtgccatc
     5521 atatcagaaa ccatatgagg cgtgtaaaat tctccggctt tcttccctgc ttctgaagca
     5581 aattgactga tcagaaattc atacgcatcc ccgatcacat ccccatcgtg acctaaaact
     5641 tccacgtcat tcaatttctt gataacttcg gtaatcgtca catttcgttg ttgctcatct
     5701 gttcctagtt tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag
     5761 gtagatgcca attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt
     5821 gcttgatccg ctaagacgtt gaaaagatac tctggtgaga tcgaataccc caaaatatca
     5881 acgattgtgg caatcaggtc ttcttttgat tcttcatctg aaagtaattc acgatacaac
     5941 attgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact
     6001 tgttccaaca aacggtcgga taaatattta tagaaaatca gtcccaataa ataattttta
     6061 tattctgacg catccatttt actacggaga ttatctgccg cactaaataa tttttgattt
     6121 aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt
     6181 attttagcat actccctttt agaaaaagca aaaaaaaaag aaaaaatctt attcttgata
     6241 acaagctacg tgacgtatat gcctaacaaa aatagaactt ttgaatcaat cttaaatgaa
     6301 ggtttaacca cagaaccggc atccattatt tttgatgaag ttatcgatga cgttgaagat
     6361 agtaagtatc ataaaaattt aaaagaactt ttttattttg cactcaacaa tggaccaagt
     6421 ttgttcactg gtatatcagt acctgatagt gataatccat taattatggc aaaaatttac
     6481 ttaacaaaat ggtgtaataa atacatcagg gataggaata acccagcatt aaaaaaacct
     6541 cttaaaacat ttggcgaaaa agatgctgct ttaactacta gagtagcagc aaatgctaat
     6601 attgatgatc aaaaagtatt gaatgactac ttgcgtggac attttttata tatgagcgct
     6661 gagaacatga acggaagtat tttagaggaa tatttagccg aagtgttaga acctgaaggt
     6721 tggatttggt gcgccggctc tgtatatcga gctgttgatt tctgttattt aggaacctca
     6781 ccaattcttt tacaagtgaa aaataaatac aatacagaaa gcagttcgag ttcagctata
     6841 agagtaggaa caacaattag aaaatggaat cgtttaaata agtcgacaaa aatttctggt
     6901 ttggatagcc ctatccccaa ttggaaagca ttaatagaaa tgactgaagc tagtaaggaa
     6961 ctggcagcta agttaactga aaatagttat ttagcttata ttaatgaaaa atccacacga
     7021 gaattatgga cactcgatga ctaacaatat ccttattagt atctatccac gatacatgga
     7081 aaaaattata aacaaacaaa aaaactttga atttagaaat ttctatctta cctcagatcc
     7141 ttataatatc ttttggattt acgaaacaat ccccaccaaa gctttaaagt ataaattgat
     7201 tgtaaaaaat ccaattacta aacttaagaa aaatcaacac tactttttag gagacgatga
     7261 gtttgaacaa ttagtttaaa agggaaaata tgcttctatc attacaagct cgtttggtgt
     7321 aagatgggta taagtcattt atgttcactc tccttgtatg ctttagcggg tattacaatt
     7381 tgagtgtaac ataaatggct tttttatttg tctcgcttaa ttatacaaac ggcgaattat
     7441 aaataactca taaaaaatat tttgactttc aaaatcactt ttgctaccag aagttgaggc
     7501 gaagtgtttt aagcatagtt gtaccctagt ttgaccgtta aaaataaata acgatcgcta
     7561 gagtgcaact atgtttgttt gccctaccgt cagcgtcggc ttggcctctc acatccatta
     7621 ccgtcgggat tatctccgtt acataccctt acaaaatacg ccctgtaagc attgcatgaa
     7681 accaccgtgc atttgcaccg tatttccgcc ctagccgttc aaatcggtcg ctaccaccat
     7741 tcagaaacac gtgcggtaat ggaaggtttg gaggaagcta tccagaccac taacgattgt
     7801 ccctatcgtc gtacgtttca ctcagatcaa ggttggtcgt atcaaatgaa agcttataga
     7861 aacaaattga aggagtacaa aatctttcaa agcatgtctc ggaaagggaa ctgcttagat
     7921 aattcactta tggagaattt ttttggctta ttaaaacaag agatttttca cgggaaagtt
     7981 tataactgtt tcgaggaatt aaaatcagcc atcgatagct atatctatta ctacaataat
     8041 gaacggataa aacagaaatt aaactggcaa agtcccgtcc aatttcgtaa aacaaccgtt
     8101 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
     8161 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
     8221 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
     8281 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
     8341 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
     8401 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
     8461 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
     8521 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
     8581 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
     8641 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
     8701 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
     8761 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
     8821 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
     8881 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
     8941 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
     9001 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
     9061 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     9121 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     9181 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     9241 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     9301 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     9361 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     9421 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     9481 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     9541 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     9601 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     9661 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     9721 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     9781 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     9841 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     9901 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     9961 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
    10021 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
    10081 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
    10141 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
    10201 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
    10261 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
    10321 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
    10381 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
    10441 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
    10501 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
    10561 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
    10621 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
    10681 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
    10741 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
    10801 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
    10861 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
    10921 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
    10981 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
    11041 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
    11101 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
    11161 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
    11221 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
    11281 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
    11341 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
    11401 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
    11461 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
    11521 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
    11581 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
    11641 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
    11701 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
    11761 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
    11821 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
    11881 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
    11941 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
    12001 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
    12061 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
    12121 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
    12181 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
    12241 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
    12301 aaacagcgat gcaattacgt gtctctccgc tgataggcaa aaagtcatga gcaatctcat
    12361 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
    12421 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
    12481 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
    12541 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
    12601 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
    12661 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
    12721 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
    12781 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
    12841 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
    12901 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
    12961 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
    13021 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
    13081 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
    13141 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
    13201 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
    13261 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
    13321 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
    13381 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
    13441 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
    13501 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
    13561 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
    13621 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
    13681 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
    13741 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
    13801 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
    13861 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
    13921 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
    13981 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
    14041 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
    14101 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
    14161 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
    14221 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
    14281 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
    14341 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
    14401 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
    14461 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
    14521 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
    14581 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
    14641 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
    14701 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
    14761 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
    14821 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
    14881 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
    14941 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
    15001 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
    15061 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
    15121 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
    15181 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
    15241 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
    15301 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
    15361 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
    15421 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
    15481 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
    15541 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
    15601 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
    15661 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
    15721 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
    15781 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
    15841 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
    15901 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
    15961 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
    16021 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
    16081 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
    16141 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
    16201 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
    16261 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
    16321 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
    16381 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
    16441 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
    16501 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
    16561 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
    16621 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
    16681 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
    16741 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
    16801 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
    16861 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
    16921 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
    16981 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
    17041 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
    17101 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
    17161 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
    17221 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
    17281 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
    17341 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
    17401 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
    17461 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
    17521 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
    17581 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
    17641 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
    17701 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
    17761 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
    17821 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
    17881 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
    17941 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
    18001 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
    18061 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    18121 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    18181 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    18241 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    18301 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    18361 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    18421 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    18481 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    18541 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    18601 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    18661 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    18721 atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc cctaaagtcc
    18781 attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcatt tagccctctt
    18841 tgcgaaggtg ttcctggatc tgcgaaaaag atggcaatat cgttcgagtt acttatagat
    18901 cgccagttgg aaaattcttt tccacaatca aatgtcatgg atttaaacaa gtgtttaggc
    18961 atggatttga accaatcatt taacctgttt tcgatatctt cggctcgtct gcctgtcggt
    19021 ttcaaagtaa taataacctt ggataaacac tcaaccaatg taataacagc gctcttgtgt
    19081 ttaactccca caatggtgtc gccttcgaga tgaccaaatt ctttagtcag gtgtggatag
    19141 agactgttcc gttcatgaat cgttctgcga aaggcctgtt ttccacgctt ttcttgatga
    19201 ttattcggtt tacgtttccc cttcataggt aacgactgtg tgtcaaaaag gcctttttta
    19261 aacatccgat agagtgtacg aacagaacaa tcaattggaa actcgccacg gccgataatc
    19321 acatcgggtg accacccttg agctaccttt tcttgaacat atgttgattg ttcatcagat
    19381 aaaacggttg aacgtcgtcc acaacgagat ttattttgtt tgtattgacg gtaataatct
    19441 agcacttttt tccttgtttg agaaacgaat aaacattata aatggtttgt ctagagcgat
    19501 ttaaactttg tgcgacaaga gcgacaggtt tctcgatatt gaaataagac tctatgatta
    19561 ctagttcgtc tgttgtaaga tgggtgtagg tcatttgtac tcactcctta ttaattcttt
    19621 ggtcggaact attttgagtg tagcacaaat gatcttttta gttgtctagc ttaattttac
    19681 aatcgacgcc tcaaaaagaa tacccgaaat ttaaacagca aaaaaagata gcgcttacca
    19741 ttgtgtttaa gataaaacgc tagctaagtt ggctagcgct ttattaatct aagcaagctc
    19801 taaaattgct tcaatacaaa atcaactgct cctttaggat cacgtgttaa attgatatat
    19861 tctgcacctt tagttgcaat tacttttatc cctaatttgt ctgcatcttt acggagatca
    19921 tccaaatagg aattcacttg aggtgctaga acgattaaat caaaattttt catcatatca
    19981 taatgagaac cataagctcc tgctttagca gaaagattaa cacctgtctc ttttgcgccc
    20041 tcttctaatg cattagccaa cattgcactc gttccggcac cagcacaaag tactaaaacg
    20101 cgctttcctt caccaaccga tgcttttgct tcatcaacta taactgccgc agttgcacca
    20161 acttcttgga tttcttcgac caattcttct tttacttctt ctgcagcaat gttttttaac
    20221 tcttgttcta ctaattcaga atcataggcc ttaacaaaag gtaaataaat aatcgcatcg
    20281 acaacaatca atactaccgc taatacaatc gccaacacac taaacccggt tcctaaaacc
    20341 aagccaatcg gtgcgggcgt cgcccaaggc aatacggata taaagctatt catccccaag
    20401 acatcgacaa aaagtttgaa tatccatacg ttagcgattg gtgctaataa aaatggaatt
    20461 agaaagtacg gattcaaaat aataggagta gcaaatagta acggttcatt taccgcaaac
    20521 gaaactggaa taaatgaagc tttaccaact gctttcaatt gttttgattt agcgaacaac
    20581 atgaacagaa aaggaacaac tagtgtcgct ccagtaccac ccatcgttcc aacaaagttt
    20641 cctagaccaa ctgttaatac ttttgaggct tgtgcaccgt ttttaaataa ttcaaagtta
    20701 gtttctacgt tcgcataaat aattgcagca atcgctggtt caacaatcga tggtccatga
    20761 acaccaacaa accagaacat tgccatggct ccccaaatga ttgcaatacc gacatatcca
    20821 tcagcagcac taaataatgg ctgcaataat tgaattacac ctgctgcaaa tgatgttcca
    20881 aaaaattgac gaactcccgt atcaatcaaa acggcaataa atactgcaat tgaaaagggg
    20941 ataatatctc taaacatctg tgtgattgtt ccgggaactt cttttggcat acggatcgtc
    21001 aaatctcttt taacacagaa tttatacaca tttacagtaa caaacgcaga aacgaaagct
    21061 gctaataatc cctttgtccc taaataatca gtagctatcc caccatcaat ttgatcagct
    21121 gccatcatta caaaaccaca aattgacgct aacataacag aaacttcatt aatctgctta
    21181 cctgaatcag ataattgacg gttcattgaa ttagctaaac atcgagctgt tgtagcaaca
    21241 accaaaagac caactaatcc cattgaataa ccataaactt tccaaagcca cgtcgagaat
    21301 gcctctggta acgtcacgtt aaatacttct ggaacagccg caatcataat aaaaatactt
    21361 gaaaatagaa tagctggcat agcagttaaa aagccatcgc gaattgcccc taaataaata
    21421 ttacgtgaga ttttttcaaa aaacggtttt cccttctcaa tttgcttaat aatcgtattc
    21481 atttttcttc tcctatgctt tagcaaattt ttctttgtac aattcaacaa aatcattgat
    21541 aagttctctt aatagaatcg ttgtcattaa atggtcttgc ccatgaataa aaattacgcc
    21601 tacttccatt tcgttccctg acgcttcttc agctaaacat ttcgtttgtg agttatgcgc
    21661 taaggttaaa ttttcatcag catccgctaa taatcgttca acattatcaa aattaccttc
    21721 tctcacttcc ttcaacactg ttaataatga acttctggca tctcctgcat aagcaacaat
    21781 ttcaaatcct aataattgca attctttcct atccatttcc ttgccctcct aactattttt
    21841 atattatcgt ttttgtttcg ctaacaaatt tataccaata tgcagactct ttcggatatc
    21901 ttttttgtgt atcgaaatct acatagaata gtccataacg cttagtgtat ccattaaccc
    21961 aagagaaaac atccattaat gaccaaatga aatacccctt tacattaact cccattttga
    22021 tagcatcgga aatcgcatta aaatgctggc gtaaataatc gattcgatca tcatctagaa
    22081 taatcccatc ttcaaaatta tccttatacc ctagcccgtt ttcagtaatg tatattttat
    22141 tataatttgg atattcgtct tggattcgta caagtaaatc atataaccct tttggataaa
    22201 tgatccaatc ccaatcggtt ttcggaagat cctcacgcac gattctctcg ccaatccctt
    22261 taatgcaata gacttggcta cctttttctc cggtagtgtt gtagtgaagt gtgctttctc
    22321 ctttgtactc tttaacccaa tggctaccat agttattgat accaagaaaa tcatttcgat
    22381 ttgctgcttt tttcatctct ctaaaatcat tagaatcaat atctgcagat tgcccatttg
    22441 ctgctaaaat ctcattaata tgtgccattg tttcatctga gtaataccct aaataagtag
    22501 catctaataa gaacttaatt gataacgcat catctaattt cgcagcatgc ttatcttctt
    22561 ctttgtcact tgctggatat tttgtctcta gcgagtgaac aacaccaatt tcccctttat
    22621 atccaccatc tttaaaaatg tttactgtct ttgcgtgcgc atacatcatg ttgtgtaaac
    22681 aagttacagt cttctctata tcaaattgaa cttctggagg aaacgatccg atcaaatatt
    22741 gatttgaagc aactggataa atctcattaa atgtactcca ataatgaacc tcagaaaatt
    22801 catcgaaaca gaactgtgca tagtttacga aatgttcaac attttctcga tttaaaaaat
    22861 ctccattatc aaatagttct ttaggggtat caaaatgatg aagagttaca aaaggaataa
    22921 taccatgctt attcaattca gcaaatacct tatgataata ttccactcct gtttggttca
    22981 cttccccata gccttttgga aaaatacggc tccatgcaat agacatccta atactatcaa
    23041 ttttaaattt tttgcataat tcagcatcaa ctggatattg attataaaaa tcactagctg
    23101 gatcagcagt atagcgtccc ttttctacaa ggaaagtatc ccacgctact tgcccttttc
    23161 caccttcttt tgtagagcct tctgcttgat aagctgccgt agctcctccc ataataaagt
    23221 cttctggtaa agttttataa ttcattttaa tctctccttg aaaattagtt tgatcaatat
    23281 gccaataatc cataaatcac ctcagcataa ttttaacatg tataagtaaa tttttttaca
    23341 caacgaaaaa ccgacctcga atatctattt tattgcgata tacaagcact tatatccctt
    23401 gatgtaaaat aaatatacca ttcaaaaacc gttttgtaaa cgtttttttg taaaataaat
    23461 ttatcttttt actaatttaa tttacacgaa acaagaaaac ggactgattc ccactaaaat
    23521 caatcccacc tactaatcat tctccaagtg gctatccatt atcgattcaa tcttctctgt
    23581 taatggatta ggctctctgc tgatatcaga gtttcgtttc ctgctagaaa aagtaattta
    23641 agttactaaa agagcagttt cgatttactt taacaaagaa aaggattatt atatccctag
    23701 gaggatgaac aatggttact attagagata ttgcaaaaaa atctggttac tcaacggcta
    23761 ctatatcaag actttttaaa ggtgatgagt cactctctat tacatctgat actaaaaaca
    23821 aaataatcgt tacagccaaa tctttaggat atgatctgac aaaaattaag actactctgt
    23881 acaagattgc cattcttttt tggatctcag aggaacagtt aaaaaaggat gtctactatc
    23941 aacatgtcaa agatgatttg aaaaattata gtaaaaaagc taatctagat ttgattttca
    24001 tctctaaaaa cgatggtgta aaaagcattc ctgacgatat tactggattt atcgcaatag
    24061 gacaattgac aaaaaaggaa ttgtactact taaatcaaaa agggtataag ggggtaatcc
    24121 gaggaataaa cccattacct gaacacttcg atactgtaga acctgatttc cacgaaatgg
    24181 ccagacaagc aattaactac tttattaatg aaggtttcac aaaaattgga tttattggag
    24241 gaaatttctt taacctagaa acgggtaaag aagagattga tagccgtgaa tatgccttcc
    24301 gatatcattt agaaaagagc ggactactca atgaaaagta tattttctcc caaggatcat
    24361 ttactgttga cgaagggtat caattaactc ttaaaatgaa agctgagtta aatgaagata
    24421 tgcccgaggc atgtttcata ccatctgaca ctatagctgc cggcgttctt cagggctgcc
    24481 atgaattggg tattaacatc cctaaagata tggcagtaat aagtattaat gatgacgaca
    24541 tcgcaaaatt cgttgcacca ccacttacaa cttttaagat agatatcgaa gagatagcta
    24601 aaacctgtgt agatttatta agtgatcaac tggtatattc gcgggacata tcaaagaaaa
    24661 cattaatcgg cgcaaaattg accgttagga aaagttttat ccctaaagaa gaggtgtaaa
    24721 ataagtaatt gaaacttatc tttaaaacta taattgtgtc cacatagtag ccgagcaatc
    24781 gggtttatgc ctattatgtt ttccataaat aatttgatgg tgatattttg acgtaaatac
    24841 aatatgattt tttcatcgcc aaattgtgcg cgctaatttt tgtttatcat tcagaacatt
    24901 tcctcctttt tatctaagta tgaggttgga taaacgcttc atcttagtat cttgggggat
    24961 tttttatctc gttgggaata tatcaagcat cagagcgcgc aaaaagaact ggtcgaaaaa
    25021 tcaatctacc agttcaatct cattcactgt gatactatgt tttatacaga taacgccata
    25081 ttataaaaca agttgtattc cgcaaatggg ttctattcaa ctcttaaact atctataaaa
    25141 gattcagcca actccattag cctctcgtaa tttagttggt gaacagcact tttttcaaaa
    25201 aattttgaac caattcctac ataagaggct ccattgttca aatagttttg tgcattagtc
    25261 tgatctaccc ctcctacagc catcaaaggt aaacttccta aaggggcttg cacttgattg
    25321 aaatattccg gtccaagaca gttggcgggg aacaccttaa caatatcagc acccatctca
    25381 aacattttga atatctctgt gggcgacatg gccccaggaa ttgttagaag gttctgttgc
    25441 gaagtttgaa aatcaaacgc gccctctctg aactccaaat atcttaggct gtta
//
LOCUS       DACDBG010000033        25087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDBG010000033 DACDBG010000000
VERSION     DACDBG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.033"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVZ90_001375"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVZ90_001375"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8262436.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVZ90_001376"
     CDS             complement(1172..1867)
                     /locus_tag="IVZ90_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8262437.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVZ90_001377"
     CDS             complement(1880..2596)
                     /locus_tag="IVZ90_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8262438.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IVZ90_001378"
     CDS             2926..3534
                     /locus_tag="IVZ90_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8262439.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IVZ90_001379"
     CDS             3859..4182
                     /locus_tag="IVZ90_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8262440.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IVZ90_001380"
     CDS             4364..5734
                     /locus_tag="IVZ90_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8262441.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IVZ90_001381"
     CDS             complement(5864..7165)
                     /locus_tag="IVZ90_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8262442.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IVZ90_001382"
     CDS             complement(7519..9510)
                     /locus_tag="IVZ90_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8262443.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IVZ90_001383"
     CDS             complement(9654..9920)
                     /locus_tag="IVZ90_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8262444.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IVZ90_001384"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IVZ90_001384"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8262445.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IVZ90_001385"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IVZ90_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8262446.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IVZ90_001386"
     CDS             13264..13827
                     /locus_tag="IVZ90_001386"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8262447.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IVZ90_001387"
     CDS             complement(14080..14601)
                     /locus_tag="IVZ90_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8262448.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IVZ90_001388"
     CDS             complement(14604..15446)
                     /locus_tag="IVZ90_001388"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8262449.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IVZ90_001389"
     CDS             complement(15439..16164)
                     /locus_tag="IVZ90_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8262450.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IVZ90_001390"
     CDS             16340..16636
                     /locus_tag="IVZ90_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262451.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IVZ90_001391"
     CDS             17204..18796
                     /locus_tag="IVZ90_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8262452.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IVZ90_001392"
     CDS             18809..19549
                     /locus_tag="IVZ90_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8262453.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IVZ90_001393"
     CDS             19717..21066
                     /locus_tag="IVZ90_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8262454.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IVZ90_001394"
     CDS             complement(21194..21784)
                     /locus_tag="IVZ90_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8262455.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="IVZ90_001395"
     CDS             22020..23099
                     /locus_tag="IVZ90_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8262456.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23320..24672
                     /gene="brnQ"
                     /locus_tag="IVZ90_001396"
     CDS             23320..24672
                     /gene="brnQ"
                     /locus_tag="IVZ90_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8262457.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         7174 a         5134 c         4886 g         7893 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat gggtggctaa cttaaacttg
    23281 aaatttacga cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt
    23341 aaagactatc tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt
    23401 atctttcccg ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt
    23461 ttcttggtta ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa
    23521 agctcaggtg tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc
    23581 atcttgcttt acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct
    23641 ttcgagattg gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt
    23701 ttcagtattg tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta
    23761 gattatgttg gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc
    23821 cttgcttttt tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa
    23881 gagcatgctt ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct
    23941 ttagcatttg ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct
    24001 gaaatcgcta aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt
    24061 tatactttgc tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat
    24121 ggcgggatca cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta
    24181 ttagcattta tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt
    24241 gcagaaacat tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct
    24301 agtatccttc cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg
    24361 cctgttttga tgttcatcta tccattagca atgacactga ttttactagt gttagttggt
    24421 cctttgttta agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct
    24481 tcgatttttg atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa
    24541 aatcttttat atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc
    24601 gtaccagcag ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa
    24661 gtagcagatt gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa
    24721 ttttgaaaaa tggcttctca catttttgca taaatttgac ttaataccga gaaactgact
    24781 tttgaacacc gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat
    24841 attttgtgaa aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg
    24901 cttttgaaca ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg
    24961 gcaaagtagc tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat
    25021 caaacaacat ttcttttata atatagattt tagacatata tatgatataa aaatagtttt
    25081 cgcaaaa
//
LOCUS       DACDBG010000034        24712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDBG010000034 DACDBG010000000
VERSION     DACDBG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.034"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            305..1531
                     /locus_tag="IVZ90_001397"
     CDS             305..1531
                     /locus_tag="IVZ90_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262458.1"
                     /translation="MIFYMFININEVLKLTTIAMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2458..3783
                     /locus_tag="IVZ90_001398"
     CDS             2458..3783
                     /locus_tag="IVZ90_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8262459.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIRASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            3776..4123
                     /locus_tag="IVZ90_001399"
     CDS             3776..4123
                     /locus_tag="IVZ90_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262460.1"
                     /translation="MIEYNDRGRLKWVGFYLSDHTERINSDNVQRANHNSAKEQMTTE
                     EIAKVLKTAVIKNRPVCIQKEERNAEGYYPDDIVGKIIGSDELGVFVEEEKVHYDEIR
                     NVSFAELVKWSQI"
     gene            4120..4302
                     /locus_tag="IVZ90_001400"
     CDS             4120..4302
                     /locus_tag="IVZ90_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262461.1"
                     /translation="MNIIKKYELGYVTYEELIEEIWGYGQQLINQVGIDCFCFYIESG
                     SGYHRYRYYIVPYPSE"
     gene            5545..6186
                     /locus_tag="IVZ90_001401"
     CDS             5545..6186
                     /locus_tag="IVZ90_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP8262462.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLNFRNALLGSDIGNSSLESIEIEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYELTIV"
     gene            6305..7390
                     /locus_tag="IVZ90_001402"
     CDS             6305..7390
                     /locus_tag="IVZ90_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8262463.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7387..9279
                     /locus_tag="IVZ90_001403"
     CDS             7387..9279
                     /locus_tag="IVZ90_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8262464.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9286..9663
                     /locus_tag="IVZ90_001404"
     CDS             9286..9663
                     /locus_tag="IVZ90_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8262465.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9817..10599
                     /locus_tag="IVZ90_001405"
     CDS             9817..10599
                     /locus_tag="IVZ90_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8262466.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10725..11456)
                     /gene="erm(A)"
                     /locus_tag="IVZ90_001406"
     CDS             complement(10725..11456)
                     /gene="erm(A)"
                     /locus_tag="IVZ90_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8262467.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11514..11601)
                     /locus_tag="IVZ90_001407"
                     /pseudo
     CDS             complement(11514..11601)
                     /locus_tag="IVZ90_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            11965..12627
                     /locus_tag="IVZ90_001408"
     CDS             11965..12627
                     /locus_tag="IVZ90_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8262468.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            12862..13005
                     /locus_tag="IVZ90_001409"
                     /pseudo
     CDS             12862..13005
                     /locus_tag="IVZ90_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13155..13355
                     /locus_tag="IVZ90_001410"
     CDS             13155..13355
                     /locus_tag="IVZ90_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8262469.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(13609..15288)
                     /locus_tag="IVZ90_001411"
     CDS             complement(13609..15288)
                     /locus_tag="IVZ90_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8262470.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15290..15502)
                     /locus_tag="IVZ90_001412"
     CDS             complement(15290..15502)
                     /locus_tag="IVZ90_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8262471.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            15895..17625
                     /gene="efrA"
                     /locus_tag="IVZ90_001413"
     CDS             15895..17625
                     /gene="efrA"
                     /locus_tag="IVZ90_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8262472.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            17622..19382
                     /gene="efrB"
                     /locus_tag="IVZ90_001414"
     CDS             17622..19382
                     /gene="efrB"
                     /locus_tag="IVZ90_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8262473.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19477..19674
                     /locus_tag="IVZ90_001415"
     CDS             19477..19674
                     /locus_tag="IVZ90_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262474.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            19827..20312
                     /locus_tag="IVZ90_001416"
     CDS             19827..20312
                     /locus_tag="IVZ90_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8262475.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20416..21009)
                     /gene="clpP"
                     /locus_tag="IVZ90_001417"
     CDS             complement(20416..21009)
                     /gene="clpP"
                     /locus_tag="IVZ90_001417"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8262476.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21189..21716
                     /locus_tag="IVZ90_001418"
     CDS             21189..21716
                     /locus_tag="IVZ90_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8262477.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            21807..22544
                     /locus_tag="IVZ90_001419"
     CDS             21807..22544
                     /locus_tag="IVZ90_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8262478.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            22548..23465
                     /gene="miaA"
                     /locus_tag="IVZ90_001420"
     CDS             22548..23465
                     /gene="miaA"
                     /locus_tag="IVZ90_001420"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8262479.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23467..24696
                     /gene="hflX"
                     /locus_tag="IVZ90_001421"
     CDS             23467..24696
                     /gene="hflX"
                     /locus_tag="IVZ90_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8262480.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8544 a         3816 c         4986 g         7366 t
ORIGIN      
        1 aataaaacag atattgagct taaagaatat aaatacaatt atttctcgag atgtatagac
       61 agaaagttac tgtttaaaaa agaaggatta gaatttagtt ttgatgaaga tgaatttgaa
      121 aaattagtga tggactggcg taagaattta ctggatacgg tacaattcta aaagcaattg
      181 aggtcgaaat tgcgatactt gaatcatgat ctatattatc gtgttatgag tgaaatttct
      241 gttaaagata agatatttca tttggacagt catgtgaata tgagcttaac aattaaacgt
      301 atttatgatc ttttatatgt ttataaacat taatgaggtg ctaaaattga caacaatagc
      361 tatgacaaaa aaaatagaag tatatgttga tggatcacaa gaagaaaggg attccttttt
      421 tgattttttt tggaaaaata gtagagaata tgtaaaagcg ttgaatctgg tatataaaca
      481 gctaatgttt gaagagttga taaaagatgc aattaaagaa atggatcaag gttatgctga
      541 gagagaagaa agtattcaaa atcggattga aaagttagca tatactgatc caatagatca
      601 aaaaaaactt gataaagcaa aagctgaatt atctaaattg agaaatgaaa aaaataaaga
      661 agcgagagaa atattaacgc aagcgatagg gttgaagagt caaacgagaa cacgagatgt
      721 agcaaaaaaa ttagactttg actttggaga ttttgttgat tcagcaaata tgaaagctag
      781 taaggatttt ggcaatgatt ttattggaat tgttactggt caaagaacac ctagaatcta
      841 tagaaataaa agtaaatacg gattgttacc gttcagaggt cgggatatta agattcacaa
      901 agctgacaag ttttatatct ctttacctaa aggatttaga cttaaagtaa atttaggtag
      961 caagccaaga aaagcaagag atgttgcttc agttttacaa cacattatgg atggcgatta
     1021 caaattaaat caaagtaatc ttatttttga tggaagaaaa ttatttttta atctaacaat
     1081 ttcttttgaa aaaagctata atgaaatgga attcaagaaa ggtaatgaat tgatcgtaaa
     1141 ctttgcagat ttgtgtagcg gtgaatgttt aattaatgat aaatctgtaa tgtcttttgg
     1201 tgatagccaa tatataagaa acttcaaaag taaaatttta gcaatgaaat caaaagaaca
     1261 aagccgagga gtcttttcta agggcggtca tggtcgaaaa agaaaattag atcaagatcg
     1321 atgggaagca ataaagaaaa gagaaagtaa ctttgttaaa acttataata atcaatgttc
     1381 tgcaaaaatc gttaaaactg ctattcgcaa taaagctcaa attattcgta tcattgaacc
     1441 agataaggct tatggcgaat gggcttacta tcaattgaaa gaacaactag taaacaaagg
     1501 aaaaagatat gggatagaag tcagaagtta gtaaattaaa caatcgttga tttggcaacg
     1561 aaatagtgag gggaaatcca ctcactgaag ggtaaactcc gtcaagttgt gtgaccagac
     1621 ggaaggacga atgttgttga ggttttagaa ctatataaaa cagcatgttt ctcaaattat
     1681 gagatgttaa gaaaaatgat tcgagaggtt ttagaactat ataaaacagc atgtttctca
     1741 aataaagtgc agaacgaaaa tcaattcatc acagttttag aactatataa aacagcatgg
     1801 ttttaaaaca agaaataagt cagttttcct tttgctttag taaggaaaaa taaagtattc
     1861 tcaataaaac ttaaaataag aaagaataag caaacctttg ttttaatagc taatcgaagt
     1921 aaaacccaaa gagccatatg aactcctgaa tcagcgaaag tttatgatga ttgggcttac
     1981 taataattta ggaattagat ggtaaatcta aattaaacaa tcgttgattt ggcaacgaaa
     2041 tagtgaggga aaatccactc actgaagggt aaactccgtc aagttgtgtg accagacgga
     2101 aggacgaatg ttgttgaggt ttagaaccac ataaaacagc atgtttctca aattgcctta
     2161 gtggttgaat tgagatctat cacgttttag aaccatataa aacaacatag ttctcaaatt
     2221 gtttaaaatg attgtcaagt taaaaaaatc tgggatcata taaaatagta tagttctcaa
     2281 atatgattag aaaccctatt aaagagcaat attaaaccaa cttacttaaa tcgtttaatt
     2341 aagaacagta atctctttta gtcagacttt tagtctgact tttttgtatt tccaaaattt
     2401 attctttaca aaaacgaaca tacgtttgta taattgcttg tgaatggggg aataaaaatg
     2461 tttgattaca gtagagaacc ggttaatgac tattttttaa tcgacatgaa gtctttctat
     2521 gctagcgtgg aatgtattga acgaaattta gacccaatga ctacaccgtt aattgtcatg
     2581 agtagatcgg ataacactgg ttcaggtctg actctagctt cttcacctac tgcaaagaaa
     2641 aaatatcata tcactaatgt tagtagagct aatgatttac cttatccatt accaaaagaa
     2701 ctttatattg ttccacctcg aatgaactta tatataaaaa aaaatatgga agttaatcag
     2761 atttttagaa agtatgtcgc agatgaagat ttattgattt attcgattga tgaatcaatt
     2821 ttaaaagtga caaaatcctt aaatttattt actagttcag aaacaagaag cgagaggaga
     2881 agagaattag ctaaaataat caaagatgag attgagaaag aaacaggact aatagcaaca
     2941 gtcggaatag gagacaaccc attgttagct aaactcgcac tggacaatga agcgaagagt
     3001 agtaaagaat ttatagctga gtggacttat gagactgtac ctgaaaaaat atggaaaata
     3061 aacgagatga ctgacttttg gggcattgga tcacgcatga aaaaaagatt gaatcatatg
     3121 ggaattatga gtatagaaga tttagcaaat tggaatccct acacaattaa aagtcagtta
     3181 ggtgtaatcg gacttcagtt atatttccat gccaatggaa ttgatcgtac ggatattgct
     3241 attccaccag aaccaaccaa agaaaagtct tatgggaatt cacaagttct accaaaagat
     3301 tatacgagga gagaagaagt agaactagta gtcaaggaga tggcagagca agtagccatt
     3361 cgaattagac aacataattg caaaactggt tgtgtacgct tagcaattgg tacgtcaatt
     3421 ttggaagaaa aatcaggttt ctcgcaccag atgaaaatac caattacaga taatacgaaa
     3481 gaattacaaa attattgtct ttttttattc aataagtttt atgaggggca ggaagtaaga
     3541 catataggag taacttatag cagattagta tacacagatt ccctacaatt agatttattt
     3601 agtgatccaa gaaaaaagat tgctgaagaa aatttggata ggattattga taagattaga
     3661 caaaaatatg gatttacagc tattatacgt gcttcatcta tgttagaagg tgcaagaagt
     3721 attacgagaa gcactttaat aggaggacac gcaggaggat taggaggttt gcataatgat
     3781 tgaatacaac gatagaggac gtttaaaatg ggtaggattt tatttgtctg atcatactga
     3841 aagaatcaat tcagacaatg tacaacgtgc caatcataac tcggcaaaag aacaaatgac
     3901 gactgaagag attgcgaaag tactgaagac tgctgtcata aaaaatagac ccgtctgcat
     3961 tcaaaaagag gaacgaaacg ctgaaggtta ctatccagat gatatagtag gaaaaattat
     4021 tggaagtgac gagcttggtg tatttgtgga agaagaaaaa gtacactatg atgaaattag
     4081 gaatgtttct tttgcggaat tagtaaaatg gagtcagata tgaacattat taagaaatac
     4141 gaattgggtt atgttaccta tgaagagcta atagaagaaa tatggggata tggtcaacaa
     4201 ttaattaatc aagtggggat tgattgcttt tgtttctata ttgaatccgg atcaggatat
     4261 catagataca gatattatat cgttccatat ccatcagaat aagttcttag agattatatt
     4321 ttcagttaat gtataatatc attttgttat caatgtgata taattgcatt actttagtag
     4381 cttgattagt aaatccagca atttttctat tattcctagt attgcaacta ctagaagtaa
     4441 ttttgctgtt tgattttcta atctttagaa gcttgattag taaatccagt aatttttcca
     4501 ttattcctag tattgctact actagaaata attttgctgt ttgattttct aatcaagctt
     4561 tttttgcttt attagaaaac gatatggatt agcagaagag tatttattac agaaaaagaa
     4621 ttagataaga tgttaccaga aaagagttct aatattgaac tcttttttta tgcctaaatt
     4681 ttttctatca attttgctat tcaataaata atttaattaa aaaaagttgt attagtagga
     4741 gaatctccta aagccaccgc aagccgttct aagcatagct tagcccgaca ttgctagatt
     4801 ttaagtgcat ctcgccataa acattttcta aagccaccgc aagccgttct aagcatagct
     4861 tagcccgaca tcgctagatt agtgcatctc accataaaca ttttctaaag ccaccgcaag
     4921 ccgttctaag catagcttag cccgacattg ctagattaag tgcatctcgc cataaacatt
     4981 ttctaaagcc accgcaaacc gtcctagcca tcgcctagtc cgattttgct tgatctgttc
     5041 ctagtgggtc aacaccgtaa ccttaaatag ccatcgccta tttaagatta ctaggtctgc
     5101 tttgaaggta aaaagtcttt cattcttcaa gacttttagg ctttcaagct acgcctgaaa
     5161 gcattggaat tcatcgctct ttatttggtt ttgccattta taattcctta cgcccattat
     5221 ctttctcgcc tgtcatacct tgtcagaagt gtttagagct tatctcatct ttggattaat
     5281 acaagtggcg taaatttttt tctaaacgca agcgttacga aaataattta cttaaacact
     5341 tgtattaatc cggcagataa gaacgcattc taatcactcc cgacaaggca agacaggcga
     5401 aaaagacaaa taaatgaacg aaaggaagaa acataaaatg caaaaccaaa tagcgatgaa
     5461 gaaaaaacca cctccctcta aaacaatcca aacaaaaaag aattttgaaa aaccaaaaat
     5521 caaaaacaga aagaagcgtt caacatgaga gatagaattt tagaagttaa tcaagaaatg
     5581 gtaaacaagt taacaaatat cagtgggaac tatgaaaagt acgatggtac tttacttgat
     5641 aactatattt ttcacgatac acaaaatatc agaattggca gaagtaaacc gagagcgttc
     5701 attctcatca aagagaaata tttgaatgaa tggaatagca cctatgaaat ggttatgact
     5761 gataactttg aaaaggtatt gaatttcaga aatgctttgt tgggttctga tattggaaat
     5821 agcagtcttg aatctattga aattgaaaat aagaccgaga atgaaggtga aggattgatt
     5881 aagaatgtct tgaaaatcga acaaaaatat atcagtgaag atagaagttt ttatagagtc
     5941 acaagctcgg ttggtctagc aaactttgct actaacttaa ttggaaacaa tgctagtgaa
     6001 tctttactag tgatgtgtct gaatacaaag aatgaggtaa ttgcttatag cgaagttttc
     6061 aaaggtggaa ttaatcaatc agtagcgata ccaagagaga tttttcaact agctttgcta
     6121 aacaattcag caaaaatcgc agtatcacat aatcatccga gcggatatga attaaccata
     6181 gtataataaa taatagtagt taaagtagtt taagaggaga tattaaaggg atattttact
     6241 ttaaataaaa attaacaagg tttataattt gtagtagtta aagtatttaa agaggtggga
     6301 acatgtgaag gttcaaagga tagaagtgga gaataagccg tatccattgt atttattact
     6361 agataaagaa taccagctaa tagaaccagt aatgaaattt attaaatact tagataatac
     6421 tggtaagtct cctaatacca ttaaagcata ctgctatcat ttaaagttgc tgtacgagtt
     6481 catggaacag agaggtgtta ttcttaatga tattaacttt gagttgttag cagacttcgt
     6541 aggttggttg agatatcctt cagcatcaaa tgtaattgat cttcagtcaa aaaaagccat
     6601 aagagaagaa acgacagtga atacaatttt aaatgtagtt atgagttttc ttgattattt
     6661 aagtagatta ggagaattta aatcaattga tgtatttaaa caagcaaagg gaagaaattt
     6721 caaaggattt ttacatcatg ttaataaggg tagataccaa aagaatgtct taaagttaag
     6781 ggttaaaaag aaacagataa gaacattgag atcagaggaa gttaagcaaa ttattgatgc
     6841 ttgtcatacg aaaagagata aattaatttt aatgcttatg tatgagggtg gtttaagaat
     6901 cggtgaagtg ttatcgctta ggcttgaaga tattgtcact tgggacaatc aaatccattt
     6961 aacacctaga gatgttaatg ttaatgaagc ttatattaaa ttaaggaagg aaagaacaat
     7021 acatgtgagt aaagaactta tgtcacttta tacagattac ttgatatatg agtatagtga
     7081 ggaattggag catgattatg tttttatttc cttaaaagaa ggctattttg ggaaaccact
     7141 aaagtaccaa agtgttcttg atctagttag aagaatagtt aaaaggactg gaatagaatt
     7201 tacatcacat atgcttcgcc acactcacgc aacgcagcta attagggaag gatgggatgt
     7261 tgcgttcgtt caaaagagat taggtcacgc acatgttcag acaacgttaa atacctatgt
     7321 tcatctttca gatcaggata tgaaaaatga gtttaataaa tacctcgaga gaaaggagca
     7381 taagaaatga atgcttctag taaaaggaaa attattagtc agagtgagat tagcaaaaaa
     7441 atagctgtaa tgaatgaaga aatgcagggg ttttgggcta ataatagttg ggatataaga
     7501 aaatgtccac atccttctgc catagaatta agtaagaatc ctgctttaag gaatcgttgg
     7561 gttcgttttg aacgtgttaa aaatctgtgg ttaagaacag aattgaaata tttttatttt
     7621 taccatttaa acaatggaat atggaatgca aaaactgtct ggattagaaa aggaacagta
     7681 attaataaaa tgttagattt cttagattta aagtatccta gcattacttc aattactgaa
     7741 gttcctattg aaaaagcaat gacggagtat agaacttatt tgacaaaacg gggtgttaga
     7801 attaccacta ctaattataa gattactgct aatcaagaaa aaacacctgt aaaagctaat
     7861 tcctactatg ttactaatct aaaacaattt atggagtttt atgagaactt ttattttgat
     7921 ggagaggagt gggataaaga cgtttgggat agacgtaact tacctttgcc agatgataag
     7981 gttaacccaa cacaatatga atatacaatt aactttaaag ggtttcggaa tacatatttt
     8041 aaacaacttg taaaaagata ttgtaagttg agattgaaca tggatagctt ttcctatgta
     8101 agtgatattg cccaaagact taaagagttc tttaattttc tggacatgaa atttaaacaa
     8161 gttcagagag tacaccaatt aacgagagtg gaaattgaag catatttaag tgaactaaac
     8221 atgatgggaa taaaacctag tacaataact gggaggatct ctatattgga aggactattt
     8281 agtacccttc ttaggctaga atgggatgat gttccttcca aaatattaat ttattctgag
     8341 gactatccga aaataccaag agcaaaacca cgctttatag atgaattcgt cctagagcaa
     8401 ttgaacagtc atcttgataa attacccgaa tatatagcta cgatgactat gattgttcaa
     8461 gaatgtggaa tgaggataag tgaattgtgc accttgaaaa aaggctgtct attagaggac
     8521 aaagatggag atttcttttt aaagtattat caatggaaaa tgaaaaagga gcatatagtt
     8581 ccaatatcta aagaggtagc tttacttatt aaagttcggg aagataaagt ttcagaggaa
     8641 tttccagata gtgaatacct ctttccaaga aaagatggat cgccattaaa acaagaaaca
     8701 tttagaggtg agttaaataa attagcttat gagcaaaata tagtggataa atcaggtgag
     8761 atttatagat tccatgccca tgcctttcgc catacagtag gaacaagaat gattaacaac
     8821 gggatgcccc agcatattgt gcagaaattt ttggggcatg aaagcccaga aatgacaagc
     8881 agatacgctc atatctttga tgaaactcta aaaaatgaat ttactaaatt tcaggaaaaa
     8941 ctggttacca ataatggaga tgtgcttgat ctagatgaag ataatgaagt cgatgatgta
     9001 gagcttcaat ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg ttattgtaga
     9061 ttgccagtag tagcaggtgg ttgtccacat gcgaatgcat gcttagattg cactcacttc
     9121 tgtaccagta agcaattctt accacagcac gaagaacagt tagagcgtac agaagagtta
     9181 ttagccatag ctaaggataa acaatggcaa agacaagtag agactaatag ccgtgttaaa
     9241 gagcgtttag aacaaatcat tggaagtttg acggggtaat tatcaatgga taaacaagtt
     9301 agaaatacaa cagaaattgt acgtttggcg aagcagaaat caaaaaagac aagggaaaaa
     9361 gtagacaaag cgatttctaa attttcgatt gaaggtaaag ttattaattt taattcaata
     9421 gcaaaggaag ctaatgtttc taaatcatgg ctttataagg aacacgatat taggcaaaga
     9481 atcgaatccc ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt
     9541 tctcgttcgg aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga attagaaaaa
     9601 gaaaataaaa aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagaa
     9661 taattattaa tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt
     9721 attgaacagt catttaggta tatgtccaat ctagggtaag taaattgagt atcaatataa
     9781 actttatatg aacataatca gttacgaggt gaaatcatga gcaatttgat taacggaaaa
     9841 ataccaaatc aagcgattca aacattaaaa atcgtaaaag atttatttgg aagttcaata
     9901 gttggagtat atctatttgg ttcagcagta aatggtggtt tacgcattaa cagcgatgta
     9961 gatgttctag tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa actaacagaa
    10021 agactaatga ctatatcagg aaagattgga aatacggatt ctgttagacc acttgaagtt
    10081 acggttataa ataggagtga agttgtccct tggcaatatc ctccaaaaag agaatttata
    10141 tacggtgagt ggctcagggg tgaatttgag aatggacaaa ttcaggaacc aagctatgat
    10201 cctgatttgg ctattgtttt agcacaagca agaaagaata gtatttctct atttggtcct
    10261 gattcttcaa gtatacttgt ctccgtacct ttgacagata ttcgaagagc aattaaggat
    10321 tctttgccag aactaattga ggggataaaa ggtgatgagc gtaatgtaat tttaacccta
    10381 gctcgaatgt ggcaaacagt gactactggt gaaattacct cgaaagatgt cgctgcagaa
    10441 tgggctatac ctcttttacc taaagagcat gtaactttac tggatatagc tagaaaaggc
    10501 tatcggggag agtgtgatga taagtgggaa ggactatatt caaaggtgaa agcactcgtt
    10561 aagtatatga aaaattctat agaaacttct ctcaattagg ctaattttat tgcaataaca
    10621 ggtgcttact tttaaaacta ctgatttatt gataaatatt gaacaatttt tgggaagaat
    10681 aaagcgtcct cttgtgaaat tagagaacgc tttattactt taatttagtg aaacaatttg
    10741 taactattga aaatagaaag aaattgttcc ttcgatagtt tattaatatt agtgacattt
    10801 gcatgcttca aagcctgtcg gaattggttt ttagtgaaaa gaacacgata ttcacggttt
    10861 acccacttat aaacaaaaga tcgatacttt ttgtagtcct tctttgaaat caatggttga
    10921 tgtcgttcaa gaacaatcaa tacagagtct acacttggct taggatgaaa atatagtggt
    10981 ggtacttttt tgagcatttt tatatccatc tccaccatta atagtaaacc caaagctcgt
    11041 tgcagatttt gcaatctttt cgcaaatccc ttctcaacga taagatagct atatttagcc
    11101 tgactttcaa aggtaattct tttgacaata tccgtactga tgttataagg aatattacca
    11161 tatatcttat agtttatatg ttttgggaag gaaaatttta gaatatccgt ttgaatcact
    11221 tttatattct cagaggggtt taccgcttct ttagtcactt gacataagcc tccatcaatt
    11281 tctatagcag taactgatcg actcattttg actagctctt tggtaaaatg tccttttcct
    11341 gatccgattt ctattacgtt gtcttgttta ctgatattcg tgtgattcaa tatttctttt
    11401 acatgctttt tagaagtaat aaaattttgc gtgtctttag ggtttttctg gttcattata
    11461 accttctcct tactggttat aatgaactga cattaagcgt tcattataac caattaattt
    11521 tgatttggtt gataacgaac tttattaata acaaatatag aaaaagtacc catactttta
    11581 tctcctttcc atttttttca ttatgaatga gataaagtaa tttctactac tgcgatactg
    11641 gtgcacataa ttgaaacctc cttatgtaac tgaatatgat tgtatcatct tttcatttta
    11701 gaataaacct ttatatgtaa ttttgcactt gacacttcag atggacgttt tcttcaaaat
    11761 aatatgtttt gaaacttgat aagagaagcc actgtcttta ctaaaaagta aaggctgcga
    11821 taaaggacag tgactttaat aaagttgcac tgttgtattg tctttggata aaattgtcta
    11881 aagggaagtt ttatccccaa cgaaaaaaca tctaaagtgg taagtaatga aagaatgtcg
    11941 ataaagtagg aatgggggat ctttttgaat acaatagaat cacagtttga taaggttgca
    12001 gaagattacg atttcgtgaa tgagcttttg aatgattatt cattctttgt gtctaatatg
    12061 tctccaaaga aaggcagagc attagatatc ggatgtggct cgggtttgtt agtggagaaa
    12121 ttagcaagtt attatgatga agtggtaggg attgatattt ctaatcaaat gctcgatctt
    12181 gccaaatcta aacgtcaact aacaaatacg gtctatctga atatgaatgc agaacaactt
    12241 aattttaatg agaagtttga ttttattgta agccgaacaa cctttcatca tttggatgat
    12301 atagccagcg taatacagca aatgaaggaa ctgttgaatg aggaaggaag aatagttatt
    12361 cttgataatg tatctgaagt cgagacaccg cctacctatg tttataaatt aggggcaatc
    12421 caagagtttt taccgcattg tttcaaattt ggaataaaaa atgcgattag aatttataat
    12481 cacaacacat ctaaatcatg gcttgagcat ttagcttcag ataagtacct atctgaacaa
    12541 aattattacg atttatatga aaagttgttg cctggatgcc aattccataa aatgggttgg
    12601 gcaatggggg ttgtctggac aaaataactt aaagctgtgg ctttgaaaag ttgaatttgt
    12661 gattgttttc agagctatcg ggtgctttag tataaataac aatgaataaa aaatcagcct
    12721 gtatcttatg ttactattaa gtaatttaag atacaggttt tactattttt gaaataagta
    12781 ccttccactt caaatacatg tattcaaagt gaaagtattg atttaaatga ttcttttgta
    12841 atttactctt gataatatga aattccatcg cagcaggata tgaattttac caaaaggata
    12901 caaaagtgtg gagaaatgat ggggatcact gtactggacc acttgatcat cggacgcaaa
    12961 cgatatttta gtttgagaga agaaggaatg atggaagaga agtaagttgc tttcttgcaa
    13021 aaaaaatgaa gcaagtgtac aattatattg taattttgtt tgggaataga agttgaaaca
    13081 tctgttgcac ctctatcgta cacgagcaat gttacaattt aaattagtgc ttttggcacg
    13141 aggaggatat tattatggaa aacggaacag taaaatggtt caacgctgaa aaaggatttg
    13201 gatttatctc acgtgaagat ggaagcgacg tattcgttca cttctcagct atccaaggtg
    13261 acggattcaa aacattagaa gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc
    13321 gcggcccaca agctgcaaac gttgtaaaag cttaatttta tctgtaaatc aaattgatgg
    13381 tagacatcaa tttttacgag agaacaaagc gagtaacaaa aatacctgcc acaagagaca
    13441 atttctcttg tggcaggtat ttttttgtat gaaaaagtaa ataaatttaa gaggctatga
    13501 aagagccacc ttgtatcaag gaataagaac aaacaaaaat ggcttctcat gtttttcatt
    13561 tgttttctct tatattcagg acaaggtgct gatttcataa cctcttattt attcttcatc
    13621 tggtgtcatt accattggca aaatcattgg atgtctttct gtacgttcga ataagaaagg
    13681 ctgaagcgcg ccagtcatcg cttctttcaa tttatattct gaacagtcag gtccattcaa
    13741 acattctctt aaagcgtgga ataagattcg ctgagcttca ttgatcattt ctcctgattc
    13801 tcgcatgtag acaaatccac gagagagaat atctggacca gccaagattt cttttttctt
    13861 gatatccact gtcgcgacag ctaatacaag accttcttct gaaagcaggt gacggtcacg
    13921 taaaacgaca ttaccaatat caccgatccc gctaccatcg atatacacat ctccagccac
    13981 aaaatgccca gcaggacgcg cagaatcgct agttaacgct aaaacatcgc cattttccat
    14041 gatgaagcag ttttctgcag gaacacctac atcttgtgct agagaagcat gtactttcaa
    14101 catgcggaat tctccgtgga caggaataaa atattttggc ttcatcaaac gaagcattaa
    14161 tttttgttct tcttgtccac cgtgaccgga agtatggatg ttattgattt ttccgtgaat
    14221 cacttctgca ccagcttcag acagcaaatt gatcaaatga ttgacacttg ttgtattacc
    14281 aggaataggt gaacttgaaa agatcactgt atcgtctggc tggatttgga tttgacggtg
    14341 agtaccattc gcgatgcggc tcaacgcggc catcgcttct ccttgagaac ctgtacatag
    14401 gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc
    14461 tggaactttg atatatccta aacgttgacc gttgacgatg gcattttcca tgcttcgtcc
    14521 gaaaacagcg attttacgac cagttgccac agcggcatct gctgcttgct gcaaacggaa
    14581 gatgttagaa gcaaagctgg caaaaatgat acgtccttcg atttttgaaa agatcttcaa
    14641 aatagaatgc ccaatcgttt tttctgattt agtaaatgtt ggaatctcag catttgtact
    14701 atcagataaa aggcacaaga ctccttcttc cccgatacga gccattttat gcaagtttgc
    14761 tggttcgcca acaggagtga aatcgaattt gaagtctcca gtcgctacga tatttccaga
    14821 aggtgttttc accacgatcc ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct
    14881 gacagaagtc tttctaaaac ggatgatcgt atcttcattg atctcatgga gctctgcatc
    14941 tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc
    15001 atagatagga atatttgctt gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg
    15061 cccgtgagta atcaccaagg cttttacttt gtgtaagttt tgtatgatgt agctgtagtc
    15121 gggaatgaca tagtcaattc caagtaaatc gtcttccggg aatttgatcc cggcatcaat
    15181 caaaatgatt tcatcttgga attgtacccc atacgtattt ttcccgattt cacctaatcc
    15241 accaatagca aagacgcctg tctcgttatt tttgatggaa agtttcatat taaaactcca
    15301 ctaatgagaa atcagcattt tctttttcgt actctagatg acttccttct agaagttgaa
    15361 cgaattcaat attgtatggg gtattttctt ccacacgttg gcgagcgtct acatcgcttt
    15421 ttgcctcaat gtaaagagat tttgtttctt cccgtttagg gttgcgtact tttgtttctt
    15481 gataataaac tttatagatc attgttttgt ctcctttatc tgcgtaaagc agtgatttag
    15541 ctagttgatt tttttagaca acacaaaacg aggggacaag ccacctggtt tttcatatgc
    15601 tttgactaga atactggatt cgagtcaaag agacaagcct cgtttttgct tatctgttcg
    15661 gatatttttt caaccgtacc ttctgtataa aaaatataca acaattaatt gttattctag
    15721 cataaaacag caaataagta tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc
    15781 atcccgctat aatcataatg acttttttat gagagaagtg ttctattaat aggctcgttg
    15841 tgctatcatt tttagtaatg aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa
    15901 ctaatgtggc attataccat gcgttataag aaattcttgt ttttcaattt catatgcgta
    15961 tttggtttta ttttgattga attaggactt cctacgattt tagcacgtat gatcgatgta
    16021 ggaatccgca atgatgattt tgattatgtg aagcaacaag ggatatttat gatcgttatt
    16081 actgtaatcg gtattatttt gaatatcttg ttaggttatt ttggttcgag gattacgact
    16141 aatatcgtag cagatattcg agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag
    16201 gaatacgaaa atctaggcgt atcttcattg atcactcgta caacaaacga tgcttatcag
    16261 atcatgttgt tcctgcaaaa tattttacgt atcggtttta tggcaccaat gatgttcatt
    16321 gtcagtttgt acatggtcat gcgtacaagt gtgacactca gtttgtatgt agtaggtgca
    16381 ttacctttac tattattggc agtcgtagcg attgctaagg tgtctgaacc gttatctaaa
    16441 aagcaacaaa aaaacttaga taaaatcaac agtatcttgc gggaaaatct ttcaggattg
    16501 cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat
    16561 gagaattatg cttctagttc aaaaagctta ttccgtttga tggctgttgc acaaccgggt
    16621 ttttatttct tatttaatat cgtaatggta ttgatcatct ggagcggggc agtgcaaatc
    16681 gatgcaggcg agcttcgtgt aggagatttg attgcgttta tcgagtatat attccatgcc
    16741 ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt atccaagagc agctgtttca
    16801 gctggacgta tccaagaagc cctagatgcc aaaccttcga ttgtagaaga tccaaatggt
    16861 atatctgata caaaaactaa agggtatatc gaatttcaaa atgtaacatt tgcttatcca
    16921 ggccattcgc aagagccagt gatacgaaat gtcagtttta cagcctctcc aggagagacc
    16981 gttgctttta ttggaagtac cggtagcgga aaatctacgt tgatccaatt gatccctcgc
    17041 ttttatgatg tgagtgaagg aaagatcttg atcgatggag tagacgtgag agattatcgg
    17101 ctaagcaagt tgcgagataa gattgggttt attccgcaaa aagcgctgct atttactgga
    17161 acgattgcag ataatttgcg atacgggaaa gaagatgcta cgcaagaaga aatggaacgt
    17221 gcagctgaga ttgctcaagc ttcagaattc atttctcgaa aaccagatgg atttaatgaa
    17281 catttatcag aaggtggttc caatttttca ggtggacaaa aacaacgatt ggcaatagcc
    17341 agagccatca tacgccgtcc ggaaatctat atttttgatg acagcttttc tgcattagat
    17401 tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag aaacgacaga atctactgta
    17461 ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag ataagatcat cgtattaaat
    17521 gaaggagaag tcgtcggtat ggggacgcat cgggagttgt tggaaaaatg cccgatttat
    17581 tatgatattg ccgcttctca attatcaaag gaggaattag catgaaaaaa accttttctt
    17641 ccttaaaacg tttagctcga tatatcagac cgtatcgtga gacttttctt ttagtgatcc
    17701 tatttacagt tctgacagtg gctttcaacg tggctttgcc atatgttaca ggacttccta
    17761 ctacagaaat cagccgtaat attgccaatg gcgaatcaat caatttttct tatatattca
    17821 aatgtctcat ttggatcaca gttgttggaa tagggtattg tatttcgcaa ttactttctg
    17881 gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga tttgagaaga gacatagaag
    17941 aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa ccagcaagga aatattttgt
    18001 ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt acaacaagct tttattggta
    18061 tcgtaaatgc gatattggga attacgttgg ctgcagcaat gatgttttat atccagccga
    18121 tgatggcgct gatctctatg atcatgattc caacatcgat ctggatctca aaacgagtga
    18181 tcaatgcttc gcagaaatat tttcaaagca tgcagaattc tcttggtgag ctaaacggct
    18241 acgtccaaga aaatatgaca ggttttagtg tattgaaagt atatggacgc gaaaaagaaa
    18301 cattggaagg attcaaaaaa gttaatcaca gcttgaaata ctttgggttt cgtgcagcat
    18361 tcatttctgg cttgatgatg cctctagttc aattaactgc ctatgctact tatattggta
    18421 tggctgttct aggaagcttt tatgttatta ctggggcgat cgttgtcggt caactacaag
    18481 cattcatcca atatatttgg caaatcagtc agccaatggg gaacgtgaca caattgtctt
    18541 cagttttgca aagtgcatca gcggcaacga aacgtgtatt cgagatttta gatgagccag
    18601 aagaaaaaga aaacaaagta gatgcttcac tgccagaaac gattttagga gatgtgacat
    18661 tcgatcatgt cgattttgca tatgatccga agaaaccatt aatccaagat ttgaattttg
    18721 aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac cggtgccggc aagacgacat
    18781 tgatcaatct attgatgcgt ttttatgacg tagacaaagg agcaataagg attgatggaa
    18841 tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag
    18901 atgcatggct ttatcaagga acgattgcag ataatatccg cttcggaaag cttgatgcga
    18961 cagattatga agtagttgat gctgcgaaaa cagcaaacgt agatcatttt atccgaacga
    19021 tgccagatgg atatgatatg gccatcaatt ctgagggaga caatgtgtct ttaggacaaa
    19081 aacagttgct gacgattgcc cgagcagtgg tctccgatcc taaaatcttg attttagatg
    19141 aagcaacaag ttcagtagac acccgtttag aagcgttgat tcaaaaagca atggatcgcg
    19201 tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag
    19261 atctgattct agtgatggat caaggtcaga tcattgaaaa aggaacgcat gagagtttat
    19321 tggcacaagg tggattttat gaaaaactgt ataacagcca atttgctgag gaagcagaat
    19381 aagtcatata agaataaaac agtttaaaac ttattttttg ttctctattc ttgataatgg
    19441 taaagtaaag ttatcaagaa aagtgaggac acggagatgg aacagttttt aaatgaaaaa
    19501 attactgaag ccaaaagaga agttgatcgt ttaacgcacg tctgtcagac gaagcctttt
    19561 gattcagctg atttttttga aaatgagttt caaatcgaac gcttggcagc acaaatagaa
    19621 gtgtacgaat taattttaaa aaaagtaata gaagaaaata tagatgtaca ataaaaacag
    19681 gtaaaacgtc agttaccaga tactgtaaat agacatctgg cctgagcagg ttttacctgt
    19741 tttttttctt atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta
    19801 caaaaaatga aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga
    19861 ttggtgatgt gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg
    19921 acagtaattg gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg
    19981 tcgatcatga tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc
    20041 tacatggctg tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc
    20101 atgcggttat tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag
    20161 ttattcctcc taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa
    20221 aagaagaaat acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc
    20281 atgcgcaagg aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga
    20341 tggtcagttt gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc
    20401 ttcctttgaa acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc
    20461 atatgctttt gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac
    20521 ttcaagcggt tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa
    20581 aatatggcgt gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg
    20641 atggatcatg atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag
    20701 caagaagctt cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac
    20761 aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat
    20821 ataaagatag atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat
    20881 cgaattagca agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa
    20941 acgtgagtag atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat
    21001 taaattcata atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt
    21061 tctaatacct aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg
    21121 caaattagtc gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga
    21181 acaacagatt gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg
    21241 tctatatcgg catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg
    21301 aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg
    21361 agatcttgca aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg
    21421 attgttatat cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt
    21481 tttatctgcc aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga
    21541 ttttatctcc agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga
    21601 ttttagagaa ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga
    21661 agactatgac gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt
    21721 ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac
    21781 aatagaactg aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg
    21841 gcacccaccc agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg
    21901 gtatcgaact agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag
    21961 aagtggaccg gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca
    22021 aaaaattgaa tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat
    22081 tgagagaagt gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa
    22141 ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa
    22201 gtagagattg gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa
    22261 tcgcattact ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga
    22321 tccaactagc agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt
    22381 tcgaacatcc agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg
    22441 atgaagaaga aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc
    22501 cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat
    22561 tagtgatcgt cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac
    22621 tatttggagg agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg
    22681 gtacagcaaa aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga
    22741 tcgaacctac tcaacgttac tcagccgctg attttcagca ttcaggtcga caattgatta
    22801 ccgagattgc gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc
    22861 aatcccttct atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa
    22921 aaaagtatga aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag
    22981 aaaaagaccc ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag
    23041 ctctggaagt tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc
    23101 aattgtatga ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa
    23161 tcaatcaaag agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt
    23221 atgagcttgg agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt
    23281 ttgaagggaa agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat
    23341 acgcgaaacg tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta
    23401 taagtgagcc aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg
    23461 aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac
    23521 ttttcagagt caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta
    23581 ttcacaatta cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa
    23641 ttacaagaat tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat
    23701 gagctaactc cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat
    23761 cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg
    23821 caggttgaac ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca
    23881 ttatctcgtc ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca
    23941 gaccgccgac atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa
    24001 gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt
    24061 attggctaca cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact
    24121 tattcgaaag atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct
    24181 gaaggatttg agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa
    24241 ctgatcgatg ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat
    24301 gtagtcgatg ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg
    24361 gatgaactga atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc
    24421 gacccagcga tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa
    24481 gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct
    24541 tatacacttt ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca
    24601 atggttttgg acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca
    24661 aaaaccaatt caaaatggtt aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DACDBG010000035        24598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDBG010000035 DACDBG010000000
VERSION     DACDBG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.035"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IVZ90_001422"
     CDS             949..1422
                     /locus_tag="IVZ90_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8262481.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IVZ90_001423"
     CDS             1431..1658
                     /locus_tag="IVZ90_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262482.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IVZ90_001424"
     CDS             2294..2665
                     /locus_tag="IVZ90_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262483.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IVZ90_001425"
     CDS             complement(2921..3163)
                     /locus_tag="IVZ90_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8262484.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IVZ90_001426"
     CDS             complement(3195..4097)
                     /locus_tag="IVZ90_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8262485.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IVZ90_001427"
     CDS             complement(4110..4298)
                     /locus_tag="IVZ90_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8262486.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IVZ90_001428"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IVZ90_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8262487.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IVZ90_001429"
     CDS             complement(4913..5806)
                     /locus_tag="IVZ90_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8262488.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IVZ90_001430"
     CDS             complement(5884..6822)
                     /locus_tag="IVZ90_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8262489.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IVZ90_001431"
     CDS             complement(6856..7206)
                     /locus_tag="IVZ90_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8262490.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IVZ90_001432"
     CDS             complement(7239..8141)
                     /locus_tag="IVZ90_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8262491.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IVZ90_001433"
     CDS             complement(8134..8991)
                     /locus_tag="IVZ90_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8262492.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IVZ90_001434"
     CDS             9325..10134
                     /locus_tag="IVZ90_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8262493.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IVZ90_001435"
     CDS             10174..10671
                     /locus_tag="IVZ90_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8262494.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IVZ90_001436"
     tRNA            complement(11056..11129)
                     /locus_tag="IVZ90_001436"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IVZ90_001437"
     CDS             11316..11639
                     /locus_tag="IVZ90_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262495.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IVZ90_001438"
     CDS             11803..12057
                     /locus_tag="IVZ90_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262496.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IVZ90_001439"
     CDS             12127..12372
                     /locus_tag="IVZ90_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262497.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IVZ90_001440"
     CDS             12448..12813
                     /locus_tag="IVZ90_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262498.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="IVZ90_001441"
     CDS             complement(12874..13437)
                     /locus_tag="IVZ90_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262499.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14005..14205)
                     /locus_tag="IVZ90_001442"
     CDS             complement(14005..14205)
                     /locus_tag="IVZ90_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8262500.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(14957..15670)
                     /locus_tag="IVZ90_001443"
     CDS             complement(14957..15670)
                     /locus_tag="IVZ90_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8262501.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="IVZ90_001444"
     CDS             complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="IVZ90_001444"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8262502.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16765..17208)
                     /locus_tag="IVZ90_001445"
     CDS             complement(16765..17208)
                     /locus_tag="IVZ90_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8262503.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17242..17595)
                     /locus_tag="IVZ90_001446"
     CDS             complement(17242..17595)
                     /locus_tag="IVZ90_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262504.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17707..18108)
                     /locus_tag="IVZ90_001447"
     CDS             complement(17707..18108)
                     /locus_tag="IVZ90_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262505.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18145..18654)
                     /locus_tag="IVZ90_001448"
     CDS             complement(18145..18654)
                     /locus_tag="IVZ90_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8262506.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18676..19533)
                     /locus_tag="IVZ90_001449"
     CDS             complement(18676..19533)
                     /locus_tag="IVZ90_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8262507.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19551..20384)
                     /locus_tag="IVZ90_001450"
     CDS             complement(19551..20384)
                     /locus_tag="IVZ90_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8262508.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20398..21195)
                     /locus_tag="IVZ90_001451"
     CDS             complement(20398..21195)
                     /locus_tag="IVZ90_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8262509.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21228..21512)
                     /locus_tag="IVZ90_001452"
     CDS             complement(21228..21512)
                     /locus_tag="IVZ90_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8262510.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21509..22510)
                     /locus_tag="IVZ90_001453"
     CDS             complement(21509..22510)
                     /locus_tag="IVZ90_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8262511.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22512..23414)
                     /gene="gnd"
                     /locus_tag="IVZ90_001454"
     CDS             complement(22512..23414)
                     /gene="gnd"
                     /locus_tag="IVZ90_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8262512.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            23578..24426
                     /locus_tag="IVZ90_001455"
     CDS             23578..24426
                     /locus_tag="IVZ90_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8262513.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         7188 a         4891 c         3989 g         8530 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa
    13981 aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg
    14041 ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc
    14101 accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc
    14161 aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct
    14221 gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa aaggagtttt
    14281 atctatgtag atatatcgct caaattacgt ttcaaaatag attactcatt taatatatca
    14341 tgctttttct aataacacaa ggataatgca atgaaatata aaattcattg tcttctattg
    14401 tcttcgaatc gaagaaaaag ttgaataacc gcgattgatt cactttttgt tttcttgttt
    14461 atttgctttt atctgcaact tttgacttta aaatttctta actatgaaaa cctttcaatt
    14521 ggtggtatac ttataaatgg aatttcgtta tcaacgcgtt cgttgccttt cagcaagtat
    14581 ttaaagaaag gagaatggct atgacattct ataacaaaga tatacaatat cgagtaacct
    14641 tggatacaga tttgaatcta tttgtcgttt ttgacaaaga aaataccaat catgtagcga
    14701 caggtgttac gatcgaacaa gcagtgcaag aattaaaaaa atctgcataa ggagtggaaa
    14761 ccctcatatg gacgattctc gttcttctct ttaaacgcat ctactggtag ataacagtag
    14821 atgcgttttg tgtttgttct aaattgttca ctacaaataa ggtagatact tcaaaataaa
    14881 aaattacttc tttttcatct gttccctcag tcgtgcagga caataaataa agaggactag
    14941 aaaatagtcc tctttattag agatacaacc ctttactttc taaaaatcgt tttgcttgat
    15001 gcaggtcata aaattcatag tctgctttat tggattcata taaacttaca tctactttca
    15061 acttatgctt cccattatga tcgggcaacc ctatcaaatg atctttgatg agaaaagaca
    15121 aaatgcccgc tcgccttgct gcttcgatcc ctgcaatcga gtcttcaaat acaagtgctt
    15181 cacttggagg aatattcatt tttgaacaag ctttcaaaaa gatctctggt tccggctttc
    15241 ctttttttac atcatcccct gaaatgattt gatcaaacaa ggcatataga ttttccattt
    15301 ttaaatacat ttctactttt acccgtgtat tagacgaagc cacgattgta tggattcctc
    15361 tttcttttgc aaaagataaa atttcttctg ctcctttttt cgtatatact ctcttatctt
    15421 ctttcacgat tttttcttta tattgattga ttgcttctcg ccatttgata tatggaacat
    15481 cttctccgta caaaatgccc atttgttctc ttatcgccat ttctgtttgg ccagttagta
    15541 aataatagac ttccttacgc atttcataat catacttttg tgctgatttt acataagcac
    15601 gataagctaa acgtccagtt tctaataaaa ggccatccat atcaaagatg aagagcttga
    15661 ttttattcat aatgctctcc cagtggtttt tccttactta tcgcctcata tagaccttgc
    15721 atgtaagtga gtcccattgc acgatcatac aggccatatc caggcatagc cacttcaccc
    15781 cacacggttc taccatgatc agggcgtata ggaccgtcga acccttcttg caccaacgct
    15841 ttcataatat gatacatatc aagggagcct tctgtagagg gatgtgccgt ttctttgaac
    15901 tttctttctc ctaaaaactc cacgtttctg aagtggacaa aatggatgcg cgaacctacc
    15961 tcatggatga tttcaactaa atcgttttcc ggatttgccc ctaaagatcc tgtgcacagc
    16021 gttagtccat tatatggaga atctactgca tttagaattt tcttcaagtc gccaagattt
    16081 ttcgtcactc taggaaaacc aaaaagttcc catggcggat catctggatg gatagccatt
    16141 tttacatctg ctttttcgca cactggaatc actttttcta aaaaataagt cagattttca
    16201 aataattctt cttcggtcat tccttcatac agtttttcca gctgagcgaa ttttttcaac
    16261 cgttcttctt cccaacccgg caatttgaat ccctttgact ggctgtgtat caattgatac
    16321 atttctcctg gctccatctg atcgacgatt ttctgatcgt ataaaagaga atagcttcca
    16381 tcgttgtttt cattaaacaa atcagttttc gcccatccaa aaattggttt gaagctataa
    16441 caaatcaatc gaaccccaca tcgagacaag tttttgattg ttcgtatata gttatcaata
    16501 tagtaatctc gttcatctgt ccctgccttg attgcatcgt ggatcgctac actttcaatt
    16561 ccaagcagtt ccagtccttg gctctcaaca gatgctttca actcctcaat ttctgaaatc
    16621 tcccaaacat ctccaggcat tttagtcagc aaagttccaa ttatcccatt tattcccggt
    16681 atttggcgaa catattttaa tgggatcgaa tccccttttt caccatacca tctaaatccc
    16741 cacttcatct tttcagcctt ccttctaaaa atcgtcctca tctaattttt tttcttccgc
    16801 tgaagcatgc caaacagtta aattatcttt tccttgtttg acaatttgtt ccaccgcttc
    16861 ttctatcggt gtgtgaagca tctgatgatt gattgcttct aacaacattg gcagattcac
    16921 gccccctatg acataagtgg ctgctttctt ttcttcagtc agttcattga ctgccaagag
    16981 cgcttgattg tacggacttg cgctaattag atcaacaaat actagcatgg gttctgctgt
    17041 gttgatcata gttaattttt ctttgacctt ctctccgaat gtttggatgt cctccccaga
    17101 ctctaagcac attgtttcaa tatggctcga tgtcccgaca attacttccg ccgcatcttt
    17161 cagtccttcg ctcaacttac catgtgtaac gatcatgatt tccaacatta gtcttctcac
    17221 tccttgtttt gcacttttta cttatcgaaa aagagcaata atctgattgg cattttgcca
    17281 attaaatgtt ttccctcgta aatctgtttt ggtcaaatag aagaactgcg cttgcttttt
    17341 tcttgcacta aaaatataat tgacgttttt ttctttcaac ttatcaactt gtttcagcca
    17401 ctgttcttta tttgaaaaat aatttcccga aaactgtagc ggtgtatctg ccccaacaga
    17461 gtctagataa aaaagaatag cctctttttt catacccgat ctcacgctgt ctaagccctt
    17521 ttctccataa cagccttcat caacaaacgc atacgctaca ctcggatcat caatcgtacg
    17581 gatcatttct agcatcgtta atatggatga agtatttcga ataaaagttt gtctgctagg
    17641 ccatccgcga gctgcttttc ccaataagaa aaagtagctc ccaaatgcta gtacagtgat
    17701 acctgtttat acggatagca gatcgaatac gtgatgagga tttttgttat atatcatcat
    17761 tccccaccaa cctaaactga acaacaaaat gaaggacaat aatatagagt agattgtttt
    17821 tttcctttgg tctttccaat caaacatgaa ataacttcct aatgcatgaa cgggtgtatc
    17881 gtaataagta cagataactt tctctgcttt ttcgatatct ccaacataaa tattttttga
    17941 tcggtaggct agcgtatcgt aagcaatgac tgtcacatct gttctcatac tgtaaatatc
    18001 tgatactaat gctgaaagaa aacgctgttt actctttttt gtacggcgct tacggtaaat
    18061 atagcgataa cgaaattccc aatcacgaaa cagctcgttc ttcttcattt tttcgccctc
    18121 tttatctttt ttctaagcat ttgtttatga caaatcaaac ttgttcaata catcttttaa
    18181 tcctgtttta ggtgcagatg gagtggcttg aaaataaata ttctttacgc catattgttc
    18241 attcatttct ttcaattttt cagcatctga tttatttaaa taaacagatt tcgtcactaa
    18301 aagatctgta tcaggatttt tttgagcaat ccctccaata ttcaattctt ccatggggat
    18361 tccgtgtttt actaagtcgt aaatcgtttg gactgttttt gcgatcaaaa tacaattgta
    18421 ttctttaaat tgatattcat cccaatagaa tttagctttc tctggtgtaa tgacaattgt
    18481 tttttgtcct gtacggcttc ctgcattttt gtaaagatct ctcataaatt tatcttttgc
    18541 tacaacttcg tctacaacaa aaatggaatt cactccactt ttttgagata acgttaccat
    18601 tacttgccca tggatcaaac gctcatccac acgcgctaaa ttgactacac ccatcttatt
    18661 cacgttccct tctttttata aaataccgat tcctgctagc acaattaata ttccagtcag
    18721 ccataataat gcttgtgtga ctttcaatcc ttttttcgta tagaacaagt acacacccat
    18781 tacgacagct aaaggtaata tccctggcac gatttgatca agaatttcct ggataacaaa
    18841 ctcacgtccg gatatcgcaa atttcaatga agattctact tttacatagt ttcccgcaag
    18901 cacccccatc ataaacaacc ctaaaactga tagcatttca atcgctgtct gaacaccagt
    18961 gttttgcttt aggcctgtag cattacggcc catagtgaaa gcttgtcttg cgaaaaatac
    19021 ccctagtaaa acctgataca acccaaagca gataaaagga aaaagtgcgc ctaaaggact
    19081 tccctgttgt gcccaaggta ccgcgattgc gataaaaata tattggacgg ttcctgaatc
    19141 gattgcatct ccgattcctg ctagtgcccc catcaatccc gctttcgtgt tatacatcaa
    19201 gtcatcctgc atcaagatct ctttttcttc atactcttct tctgctcttt gctgttccat
    19261 agaagacatt aggccagtaa tcacaccgcc accccagctt aattgagtgt tgaagaacag
    19321 taactgccgt ttatatgctg cttttaaaga gtcttcatct ttataaagtt ttttcaagat
    19381 tggcatcatt ccgtatagta gtgatggcgc caagtaacgt tcaaaagagt gaggaatttc
    19441 gtttgcaaaa tgccatctca gataggcttt tgttacatct tttttcgtga tttcttcagg
    19501 agcacctgtt tgattatcca ttatttcagc catttcccat tcctcctaaa ttaaaatccg
    19561 tcatcatagt catcttcgtc atcaaaagat gctgaattgt tattgcttgt ggcaggctgt
    19621 acttgtttcc ctttttgcga ctggacaaag atcaaagcga taataatccc aaaaatggca
    19681 taagtaacca tcgtaatatc taaagatttc aaaacaacac tcataaaata agcaaggaag
    19741 aaaaagacca gataatcttt tcgaccgata acgtatacag ttgtcgcgat accgattgcc
    19801 gctaaccctc cacctactac ttcaagaatg tggataacga ctgttccctc taaagctttg
    19861 attacatcag cgatcaatgg cgcccctaaa tataaagcaa tgaaaacaag cggaacgaat
    19921 aataaaaatg aggcaaccgt tggataaaga ataattgatc gggccatagc ttttgtattc
    19981 aaatcttcta cagctttgtc tgtatattta cctaaaaaag tattgatgaa aaaacgaaac
    20041 tgatacaagt aacttccgag caatccgact ggcaccgcaa cggcaatcgc tgcttctggc
    20101 gacagatccc ctaataatgc gactggtact gcgatagctg ctgcaataga tggttcagct
    20161 ggcattgatc ctcctggaga aaaaaccccc atataaatca tttgcaaagc ggctgtaaca
    20221 atcatcgctt gtgcaacttc ccccatgatc aacccgacga ctaatccagt catcaaagga
    20281 gaaaaacgta aggtcaacgt ggctcctcca cctaaccagc gggacataac cgcggctacc
    20341 cataaagcaa tcaacaaact ttgtactaca cttaatgttt ccatgtgtcc tcctccttca
    20401 gatctttctg atataacctt ccagttccat cattgactgt atcagtgatt tctgtgttac
    20461 ctctatagga atcaaatgca ctttctcttt cgaatcaata aattcagcca ttttatctat
    20521 tatctgttta tctttcggaa ataatcccat ttgttctaga gaaaccggca gttctaattc
    20581 ccgatacaaa ggatataact cgtctatttt ttcccagcga ccttctagtg ccagttgata
    20641 gaaaatccca tacgctactt tttctccatg tagaaaacga tggctttccg gaatatattt
    20701 actgatagcg tcatgcatag catgtgctgc cacatttctt gcgtatttat ctcctaatcc
    20761 tccaactaaa ccagcaacag caaagataat ctctgaaagc tgtttgaact ccgatgtcag
    20821 ttttctttgt gccatatctt ggattgcttt agcagaatga ttcaaaatct cttctttgca
    20881 gatcttcgca gtatcttgtg ctagcttcaa aaatgattca tcttgcaaaa aatcttgttc
    20941 taagatgagc tcagattcat accattttgc tagtgtatct gctaacccgg caatgaaata
    21001 tggaacaggg gaatcaatca ctaattgggg atctgttata aaaaaagcag cttgtctgta
    21061 aaagtgttct gttttccctt ctgcttgtcc attctcctta tacatgacag caagaggtgt
    21121 ccatggagca caattactag ctaaagtagg gatgactccg aaatcgattc ttgcaagatt
    21181 ggcactataa cccactagat cggtcagttt tcctccgcca acgccaacta cgaaatcgat
    21241 ttcatttttt tgatgattgt agctattcgt ctggcttcag cataactaca ttctccagaa
    21301 taacgatgat aaaaaaattg aatcgtttcg tcattcaaaa atactaattt aggtagcgcc
    21361 ttttcccatg ataccgttcc gtgggtgacc agtactcttt tcgcgccgta tgatttcaag
    21421 agatcagaaa cttttgaaat accaccttct tcatataaat aaaattgagg gcctgttttc
    21481 acttttaaac cgttcaccat tttcttcatc atccttttat atagttgctc tttgtctgga
    21541 cagaacgttc aggaagaagg tgctgactga tctgttcgac atctctgaca catttttcac
    21601 ccattgcttc taaacattcc cttgtgtagg ccgatatatg tggcgtaatc actacttgtt
    21661 caaacgctaa atacggatga tcttgacgtc ccggttcttc ttctaaaaca tctgtggcat
    21721 atcctgcgat ctttccgctt ttcagaccat taatcatcgc ttcttcatca actagagcgc
    21781 ctcttgcact attagataga taaacatttt ctttcatctg atcgacttct ttatcagaaa
    21841 tcatgtgata gttctcttct gtcaaattcg cacataaaca aatgatatct gattgttcca
    21901 gtaattcttg aagttctact ttttttgcac caaagctttc gatgtataaa tctgatttgt
    21961 aaggatcata agctagaaca tcacagcgaa aaccgttacg caaaatttcg attactccgc
    22021 ttccggtatt tcctgcacca atcacaccaa ctgttttgtt aaacagcgtc ctaccgacga
    22081 actccgcccg atcttcccat cgatcgtcct tgactcttgc ttgtgcttgt gtcgtgcgtc
    22141 gaagcatgga caacaaatta gttatattat tttctgctac agcatttcgt tcaactaaag
    22201 caggtatgat agatacgatt gtctggtgtt cttttgctgc ttttaaatca atattgttgt
    22261 aaccaatacc atgtcgggaa atcaatagca gttcatcttt gttttcaaaa aaatctcgtc
    22321 gaaaaaatgg cgtaacactt gcaataatga tattgaatcc tttaagtaac tccgctagtt
    22381 cttttcctgg aatctcttga tcaacagtaa agaaatgaac gtctccaact gactttaatc
    22441 tttccaagtg gtgtggaaag actcttccga aactgctgga attaacgatt gctatatgat
    22501 aatctggcat tttactcacc cttttcaact atttcatgtc cgccaaaccc atttcttagt
    22561 gctgaaacta cttttgctga aaaactatct tcttcctgtg aaagattcct catgaacaaa
    22621 ctagttgcaa ttactggaac tggaacagcc atttctagcg ctgtttcaac cgtccatttt
    22681 gcttcacctg atgcagaaac gattccccga taattactta aattgggatg ttcgtttaac
    22741 tgatcttcca tgatttccat cagccagccg cgaatcaccg aaccatgatt ccaaacacgt
    22801 gcgacttgtt ccaagtcaaa agcatactca cttttttgta caagttggaa tccttcaccg
    22861 attgcttgca tcattccata ttcgattcca ttatggatca ttttcaagaa gtgtccgctt
    22921 ccggatgcac cagcatacag atatcctctt tcaattgcta catctttgaa taattgttcc
    22981 acggatagaa aagtttcttt atctcctcca atcattaagc aggcttgatg tcgtgctcct
    23041 tcgattcctc cagaagtccc acaatccaag taaccgattt catttttctg aaactcttct
    23101 gctcgacgga ttgaatcttt ataataagag tttccgcctt caataataat gtctcctggt
    23161 ccgagttttg gaaaaagttt tcttaacatg ttttcggtta tctctccagc aggaagcatt
    23221 aaccagatga tttttttctg ctcaaaagta gctagaaatt cagagatctc ttgataagca
    23281 ggaatgtcat tttctcttgc ctgactaaca gctgtacttg aaatgtcgag tccttgtact
    23341 ttgtgatggt gctcttttaa gtttaaagct aaattcaatc ccatttttcc caaaccaatt
    23401 acaccaattt ccatgatagc gcctccattt cttataccct cattatacaa aaaaattttt
    23461 ttgtaaatag attttacaaa aatatttttt gtttgatttt ttcctactct ccttgtatct
    23521 ttaacattaa tgagaacgga aaaaatgata atcttattat gaaaatgagg aattctcatg
    23581 agcgtctcca taagactaca aatcaaaaca atgtacaacg acttcagccc aaaagaacag
    23641 gctatcgccg attatatttt agaaaatcca agcaaagtaa gtcatagtcc tattagcaat
    23701 ttagcaagtg aactgggcat tgctgactct actttttttc aatttacaaa aaaacttggt
    23761 tataatggat ttaaagattt taaaatggcc atgttgatcc aagaaaatga tctatctgct
    23821 atttctatcc acgaaaatat tcagaaaaca gatactgagc tcactatggc gcaaaaggtg
    23881 ttcgagtcta gtatttcaac tttaaatgac acaaaaaagt tgttacaaca aaaggatcta
    23941 aaaaaagcgg ctactattat caatgaatct aaacgtgtct attttttcgg tgttggcggt
    24001 tcagagattg tagcaactga cgcttatcat aaatttttgc gttcccctat cgcaacttct
    24061 cacagcacgg attaccatat tcaattgatg gaagcttctt tattgacgga aaaagactgt
    24121 gctgttttga tttctcacac agggcaatca aaagaaacaa tccatattgc tgaaaccgta
    24181 agaaaaactg gagcaaaaac aatcgttatt actagccaag ctaattcttc ccttgctaaa
    24241 cttggcgatg tcgtttttat ttctatttct gaagaaacag aattccgatc tgaagcttta
    24301 gcttcccgta tttctcaact aagcattttg gactctctat atgtgatttt gatgttttac
    24361 aacgaaaaag aatcacgtcg ttctatcgct aaagttcgac atactatttc tgaaatcaaa
    24421 gaatagcacc ataacagaaa aagcgaaaaa tccataaaac aaaaaaagcc actaccaaat
    24481 atttggtagt ggcttttttg gttctgcaag ggtggtacag aacatcaata gtcggtgggt
    24541 tagaacgtat gtcatcgcat ctttggagaa ggttgtattg gtattattcg agttactt
//
LOCUS       DACDBG010000036        24270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDBG010000036 DACDBG010000000
VERSION     DACDBG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.036"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..571
                     /locus_tag="IVZ90_001456"
     CDS             <1..571
                     /locus_tag="IVZ90_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8262514.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            575..1216
                     /locus_tag="IVZ90_001457"
     CDS             575..1216
                     /locus_tag="IVZ90_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262515.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1213..2130
                     /locus_tag="IVZ90_001458"
     CDS             1213..2130
                     /locus_tag="IVZ90_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8262516.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2127..2798
                     /locus_tag="IVZ90_001459"
     CDS             2127..2798
                     /locus_tag="IVZ90_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262517.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3339..3827)
                     /locus_tag="IVZ90_001460"
     CDS             complement(3339..3827)
                     /locus_tag="IVZ90_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8262518.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4028..4294)
                     /locus_tag="IVZ90_001461"
     CDS             complement(4028..4294)
                     /locus_tag="IVZ90_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8262519.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4294..4557)
                     /locus_tag="IVZ90_001462"
     CDS             complement(4294..4557)
                     /locus_tag="IVZ90_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8262520.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4550..4822)
                     /locus_tag="IVZ90_001463"
     CDS             complement(4550..4822)
                     /locus_tag="IVZ90_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262521.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5033..5218
                     /locus_tag="IVZ90_001464"
     CDS             5033..5218
                     /locus_tag="IVZ90_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8262522.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5360..7183)
                     /locus_tag="IVZ90_001465"
     CDS             complement(5360..7183)
                     /locus_tag="IVZ90_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8262523.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7781..10486)
                     /locus_tag="IVZ90_001466"
     CDS             complement(7781..10486)
                     /locus_tag="IVZ90_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8262524.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEVGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10792..11739)
                     /locus_tag="IVZ90_001467"
     CDS             complement(10792..11739)
                     /locus_tag="IVZ90_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8262525.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11929..12198
                     /gene="rpsN"
                     /locus_tag="IVZ90_001468"
     CDS             11929..12198
                     /gene="rpsN"
                     /locus_tag="IVZ90_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8262526.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12374..12736)
                     /locus_tag="IVZ90_001469"
     CDS             complement(12374..12736)
                     /locus_tag="IVZ90_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8262527.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12747..13868)
                     /locus_tag="IVZ90_001470"
     CDS             complement(12747..13868)
                     /locus_tag="IVZ90_001470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8262528.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13884..14234)
                     /locus_tag="IVZ90_001471"
     CDS             complement(13884..14234)
                     /locus_tag="IVZ90_001471"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8262529.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14364..15875)
                     /locus_tag="IVZ90_001472"
     CDS             complement(14364..15875)
                     /locus_tag="IVZ90_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8262530.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16197..17549)
                     /locus_tag="IVZ90_001473"
     CDS             complement(16197..17549)
                     /locus_tag="IVZ90_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8262531.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17702..18778)
                     /locus_tag="IVZ90_001474"
     CDS             complement(17702..18778)
                     /locus_tag="IVZ90_001474"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8262532.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18974..20719
                     /locus_tag="IVZ90_001475"
     CDS             18974..20719
                     /locus_tag="IVZ90_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8262533.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20999..21436)
                     /locus_tag="IVZ90_001476"
     CDS             complement(20999..21436)
                     /locus_tag="IVZ90_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8262534.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21420..22793)
                     /locus_tag="IVZ90_001477"
     CDS             complement(21420..22793)
                     /locus_tag="IVZ90_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262535.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23115..23603)
                     /locus_tag="IVZ90_001478"
     CDS             complement(23115..23603)
                     /locus_tag="IVZ90_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8262536.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23625..24227)
                     /locus_tag="IVZ90_001479"
     CDS             complement(23625..24227)
                     /locus_tag="IVZ90_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8262537.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6962 a         5165 c         4485 g         7658 t
ORIGIN      
        1 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa
       61 tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact
      121 acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat
      181 tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac
      241 gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa
      301 taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt
      361 aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata
      421 cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt
      481 ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc
      541 agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg
      601 ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat
      661 tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac
      721 gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt
      781 gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc
      841 attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg
      901 ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc
      961 cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg
     1021 ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact
     1081 gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact
     1141 attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga
     1201 ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata
     1261 cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc
     1321 acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa
     1381 cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat
     1441 caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta
     1501 acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa
     1561 gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatagttt tgaaaatacg
     1621 tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat
     1681 ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa
     1741 ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct
     1801 atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc
     1861 tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa
     1921 ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct
     1981 catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa
     2041 ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta
     2101 caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac
     2161 aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact
     2221 tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca
     2281 tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga
     2341 cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg
     2401 tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg
     2461 ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta
     2521 gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa
     2581 gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg
     2641 gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag
     2701 caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg
     2761 aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc
     2821 ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa
     2881 tccactctga ttcttccctc cttatgggta aagagttgct tttcttagct atttatccat
     2941 tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt
     3001 ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca
     3061 taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca
     3121 tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc
     3181 agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat
     3241 cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc
     3301 atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa
     3361 ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg
     3421 atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg
     3481 ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg
     3541 acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta
     3601 ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa
     3661 taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga
     3721 taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact
     3781 gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct
     3841 tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt
     3901 ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa
     3961 caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct
     4021 tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat
     4081 caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg
     4141 taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa
     4201 tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg
     4261 cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc
     4321 gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt
     4381 ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc
     4441 attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt
     4501 caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt
     4561 ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca
     4621 ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt
     4681 ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac
     4741 gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt
     4801 ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt
     4861 gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg
     4921 tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac
     4981 tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact
     5041 gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac
     5101 atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt
     5161 tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat
     5221 aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct
     5281 ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt
     5341 tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat
     5401 tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct
     5461 gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg
     5521 ctgcattttt acttacgata tctacataac ggataattgg attttcaatc gagcgataag
     5581 aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt
     5641 ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga
     5701 tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa
     5761 gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg
     5821 ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga
     5881 tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg
     5941 agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt
     6001 tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt
     6061 ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta
     6121 gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt
     6181 gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg
     6241 tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac
     6301 catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga
     6361 tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca
     6421 tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat
     6481 tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca
     6541 aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag
     6601 cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg
     6661 tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca
     6721 tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg
     6781 ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga
     6841 tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga
     6901 catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg
     6961 aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag
     7021 acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat
     7081 cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag
     7141 attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac
     7201 acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac
     7261 gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat
     7321 atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt
     7381 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
     7441 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
     7501 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
     7561 gcggtaataa gcccaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
     7621 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
     7681 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     7741 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
     7801 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     7861 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
     7921 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     7981 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     8041 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     8101 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
     8161 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
     8221 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
     8281 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
     8341 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
     8401 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
     8461 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
     8521 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
     8581 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
     8641 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
     8701 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
     8761 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
     8821 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
     8881 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
     8941 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
     9001 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
     9061 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
     9121 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
     9181 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
     9241 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
     9301 tgctgatgtt tttcttttca tcatcagtca gcttaccaac ttcaatagct gttcttacag
     9361 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
     9421 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
     9481 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
     9541 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
     9601 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
     9661 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
     9721 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
     9781 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
     9841 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
     9901 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
     9961 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    10021 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    10081 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    10141 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    10201 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    10261 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    10321 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    10381 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    10441 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    10501 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    10561 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    10621 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    10681 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    10741 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    10801 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    10861 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    10921 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    10981 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    11041 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    11101 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
    11161 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    11221 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    11281 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    11341 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    11401 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    11461 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    11521 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    11581 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    11641 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    11701 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    11761 ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    11821 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
    11881 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    11941 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    12001 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
    12061 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    12121 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    12181 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    12241 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    12301 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    12361 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    12421 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    12481 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    12541 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    12601 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    12661 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    12721 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    12781 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    12841 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    12901 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    12961 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    13021 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    13081 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    13141 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
    13201 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    13261 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
    13321 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    13381 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    13441 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    13501 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
    13561 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    13621 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    13681 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    13741 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    13801 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    13861 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    13921 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    13981 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    14041 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    14101 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    14161 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    14221 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    14281 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    14341 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    14401 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    14461 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    14521 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    14581 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    14641 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    14701 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    14761 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    14821 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    14881 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    14941 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    15001 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    15061 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    15121 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
    15181 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    15241 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    15301 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    15361 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    15421 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
    15481 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    15541 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    15601 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    15661 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    15721 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    15781 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    15841 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    15901 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    15961 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    16021 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    16081 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    16141 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    16201 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
    16261 ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct
    16321 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
    16381 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
    16441 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
    16501 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    16561 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    16621 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
    16681 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    16741 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    16801 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    16861 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
    16921 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    16981 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
    17041 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    17101 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    17161 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    17221 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    17281 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
    17341 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    17401 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    17461 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    17521 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    17581 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    17641 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
    17701 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
    17761 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    17821 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    17881 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
    17941 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    18001 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    18061 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    18121 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    18181 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
    18241 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    18301 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    18361 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    18421 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    18481 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    18541 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    18601 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    18661 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    18721 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    18781 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    18841 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
    18901 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    18961 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
    19021 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    19081 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    19141 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    19201 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    19261 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    19321 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
    19381 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    19441 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    19501 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    19561 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
    19621 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
    19681 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
    19741 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
    19801 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
    19861 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
    19921 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
    19981 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
    20041 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    20101 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    20161 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
    20221 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    20281 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
    20341 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    20401 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
    20461 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    20521 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    20581 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    20641 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    20701 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    20761 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    20821 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    20881 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    20941 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    21001 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    21061 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    21121 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    21181 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    21241 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    21301 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    21361 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    21421 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    21481 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    21541 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    21601 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    21661 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    21721 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    21781 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    21841 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    21901 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    21961 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    22021 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    22081 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    22141 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    22201 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    22261 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    22321 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    22381 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    22441 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    22501 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    22561 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    22621 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    22681 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    22741 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    22801 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    22861 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    22921 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    22981 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    23041 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    23101 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    23161 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    23221 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    23281 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    23341 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    23401 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    23461 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    23521 ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    23581 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    23641 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag
    23701 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    23761 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    23821 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    23881 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    23941 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    24001 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    24061 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    24121 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    24181 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    24241 atcttttttc atttattaaa actgcctcct
//
LOCUS       DACDBG010000037        23831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDBG010000037 DACDBG010000000
VERSION     DACDBG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.037"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IVZ90_001480"
     CDS             complement(55..789)
                     /locus_tag="IVZ90_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8262538.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IVZ90_001481"
     CDS             1116..1526
                     /locus_tag="IVZ90_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8262539.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IVZ90_001482"
     CDS             1519..2220
                     /locus_tag="IVZ90_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8262540.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IVZ90_001483"
     CDS             2220..3806
                     /locus_tag="IVZ90_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8262541.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IVZ90_001484"
     CDS             3825..4046
                     /locus_tag="IVZ90_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8262542.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IVZ90_001485"
     CDS             4043..4804
                     /locus_tag="IVZ90_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8262543.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IVZ90_001486"
     CDS             5184..5693
                     /locus_tag="IVZ90_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8262544.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IVZ90_001487"
     CDS             complement(5768..6307)
                     /locus_tag="IVZ90_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8262545.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IVZ90_001488"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IVZ90_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8262546.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IVZ90_001489"
     CDS             7374..8171
                     /locus_tag="IVZ90_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8262547.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IVZ90_001490"
     CDS             8199..8834
                     /locus_tag="IVZ90_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8262548.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IVZ90_001491"
     CDS             8854..9627
                     /locus_tag="IVZ90_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8262549.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IVZ90_001492"
     CDS             10035..10832
                     /locus_tag="IVZ90_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8262550.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IVZ90_001493"
     CDS             10810..11223
                     /locus_tag="IVZ90_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262551.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IVZ90_001494"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IVZ90_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8262552.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IVZ90_001495"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IVZ90_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8262553.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IVZ90_001496"
     CDS             16000..16560
                     /locus_tag="IVZ90_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8262554.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IVZ90_001497"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IVZ90_001497"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8262555.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IVZ90_001498"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IVZ90_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8262556.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IVZ90_001499"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IVZ90_001499"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8262557.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IVZ90_001500"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IVZ90_001500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8262558.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IVZ90_001501"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IVZ90_001501"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8262559.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IVZ90_001502"
     CDS             21907..22485
                     /locus_tag="IVZ90_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8262560.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IVZ90_001503"
     CDS             22537..23019
                     /locus_tag="IVZ90_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8262561.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IVZ90_001504"
     CDS             23136..23510
                     /locus_tag="IVZ90_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262562.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DACDBG010000038        23786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDBG010000038 DACDBG010000000
VERSION     DACDBG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.038"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..727
                     /locus_tag="IVZ90_001505"
     CDS             2..727
                     /locus_tag="IVZ90_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262563.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            717..1226
                     /locus_tag="IVZ90_001506"
     CDS             717..1226
                     /locus_tag="IVZ90_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262564.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1296..2744)
                     /locus_tag="IVZ90_001507"
     CDS             complement(1296..2744)
                     /locus_tag="IVZ90_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8262565.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2744..3460)
                     /locus_tag="IVZ90_001508"
     CDS             complement(2744..3460)
                     /locus_tag="IVZ90_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262566.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3441..3791)
                     /locus_tag="IVZ90_001509"
     CDS             complement(3441..3791)
                     /locus_tag="IVZ90_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8262567.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3935..4708
                     /locus_tag="IVZ90_001510"
     CDS             3935..4708
                     /locus_tag="IVZ90_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8262568.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4879..5245
                     /gene="ssrA"
                     /locus_tag="IVZ90_001511"
     tmRNA           4879..5245
                     /gene="ssrA"
                     /locus_tag="IVZ90_001511"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5679..5993)
                     /locus_tag="IVZ90_001512"
     CDS             complement(5679..5993)
                     /locus_tag="IVZ90_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262569.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            6279..6518
                     /locus_tag="IVZ90_001513"
     CDS             6279..6518
                     /locus_tag="IVZ90_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262570.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(6880..7140)
                     /locus_tag="IVZ90_001514"
     CDS             complement(6880..7140)
                     /locus_tag="IVZ90_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262571.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7325..7822)
                     /locus_tag="IVZ90_001515"
     CDS             complement(7325..7822)
                     /locus_tag="IVZ90_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8262572.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(7952..8662)
                     /gene="budA"
                     /locus_tag="IVZ90_001516"
     CDS             complement(7952..8662)
                     /gene="budA"
                     /locus_tag="IVZ90_001516"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8262573.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(8675..10339)
                     /gene="alsS"
                     /locus_tag="IVZ90_001517"
     CDS             complement(8675..10339)
                     /gene="alsS"
                     /locus_tag="IVZ90_001517"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8262574.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(10547..11209)
                     /locus_tag="IVZ90_001518"
     CDS             complement(10547..11209)
                     /locus_tag="IVZ90_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8262575.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(11219..12034)
                     /locus_tag="IVZ90_001519"
     CDS             complement(11219..12034)
                     /locus_tag="IVZ90_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262576.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(12295..12738)
                     /locus_tag="IVZ90_001520"
     CDS             complement(12295..12738)
                     /locus_tag="IVZ90_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8262577.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(12872..13210)
                     /locus_tag="IVZ90_001521"
                     /pseudo
     CDS             complement(12872..13210)
                     /locus_tag="IVZ90_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(13198..13575)
                     /locus_tag="IVZ90_001522"
     CDS             complement(13198..13575)
                     /locus_tag="IVZ90_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8262578.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            13799..14992
                     /locus_tag="IVZ90_001523"
     CDS             13799..14992
                     /locus_tag="IVZ90_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262579.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(15158..15580)
                     /locus_tag="IVZ90_001524"
     CDS             complement(15158..15580)
                     /locus_tag="IVZ90_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8262580.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            16163..16657
                     /locus_tag="IVZ90_001525"
     CDS             16163..16657
                     /locus_tag="IVZ90_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262581.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(16819..17991)
                     /locus_tag="IVZ90_001526"
     CDS             complement(16819..17991)
                     /locus_tag="IVZ90_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8262582.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(18197..19012)
                     /locus_tag="IVZ90_001527"
     CDS             complement(18197..19012)
                     /locus_tag="IVZ90_001527"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8262583.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(19162..19608)
                     /locus_tag="IVZ90_001528"
     CDS             complement(19162..19608)
                     /locus_tag="IVZ90_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8262584.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(19605..20609)
                     /locus_tag="IVZ90_001529"
     CDS             complement(19605..20609)
                     /locus_tag="IVZ90_001529"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8262585.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(20649..20978)
                     /locus_tag="IVZ90_001530"
     CDS             complement(20649..20978)
                     /locus_tag="IVZ90_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8262586.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(21104..23431)
                     /locus_tag="IVZ90_001531"
     CDS             complement(21104..23431)
                     /locus_tag="IVZ90_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8262587.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         6942 a         4657 c         4125 g         8062 t
ORIGIN      
        1 tatgaaaata atccgctatg ctactgctcg attaatttat tacaaaaagc agacaatact
       61 ctattgttta ttttcaagct tttctgcttt tttattgata gcatgcctga ctctttacca
      121 tttgcaatcg gctttagcca atcaagtcca agatcggctt tcatttattg gcgcttctgc
      181 atctacagag cttcttccgg ctttagaatt agctagccga ttttatataa aaggcatatt
      241 cagtttattt tttctatttg ccttcctttt tatctttttc ttttactatt cagtcaggca
      301 aaacaagcag gaattgatca attggcgcct gactggtctt tctaaaacca aattattcct
      361 ttttattttc tggcagctgt tctttcctct ctttctttgt tgtatcttgg ttttattgtg
      421 gatcgtaagc ttccagtcag tttatgaagc gcttcttcaa aggatcaatt ttcttgtctt
      481 aaagactact gaactgccag atatccgtga tatcttgact tcggtaagcg gacttacaat
      541 tccgatggat caacagactt tttttaccat cgatttccaa aatgatcttt ttttcacaga
      601 tatacttaag ggatttgccc aaactcttat tgggctcagt ggatgctgtc tcattcttag
      661 tctattccct ttcacttttt ttatccatca tttgcaaagt aggaggcctg gacgtaatga
      721 aacctgaata tttgattcat gctgaacact tgaccgttat tttgtgtcct ctcgaaaaac
      781 agcataaaat attggagcac ttgaaaaaag aggttcttgt tgcaaaactg gataattttg
      841 cgatcatcca aaaaaattgg ccgatgtttc cttatctgaa tcttaaagac catgtcctgt
      901 tggatgtacc ggaaaaagaa atcaaacaag atcgtttggc ctatcaagaa aaacttgata
      961 tctctccttc cttgcttaat tgtgcggcag ctgagttgac gtcttttgaa aaagtaaagc
     1021 tgcaattact tcatgccttg ctttccaagc gaaacaatat tgttattgaa gatacatttg
     1081 atgaacttag tgtattggag atccaagagc tactccactt gctttcttat ttagctcatg
     1141 aagaaaatca aggtatctta ctctttactc atgatgctac tattgctcaa agtccttata
     1201 ttgatcgttt agagcccgcg ggctaaatca atcattttat agtaaaaaca agcggtatag
     1261 aagatcgatt catgatcttt tataccgctt gttttttact cttttttgat ttctacgtca
     1321 ggcaaacgaa cagcgaattt tgttcctttt cctagttcgc tttctacttc taattttcct
     1381 ttatgcaagc gtacaagttg ctgcacgatt ggtaaaccta atcctgattc accatatttt
     1441 gtattttttc ttgaaggatc tactttataa taacgatccc agatactttt catttgttgt
     1501 tcagacattc caattcccgt atcttcaatc gtgatgatcg tttcaagata gcctttttct
     1561 aaagtaatcg tgatttggcc gttttcagta aactgtatcg cgttttgaat aatattgacc
     1621 atgatttgga caaatcggtc ataatcagcg taaacatcaa tatcatcaac ggctttcaat
     1681 gtcaatgtat ctcctgctgc ttctgcttta gcttctaact gtgcaacaat atttttcaat
     1741 gtttctgttc cattgaattt tttaatgacc atactgattt gattcgtacg gattttttca
     1801 taatccaagt tttcattcac caaacggatc agacgctctg tttcattttt catcaattta
     1861 atcgcgtttt ctcgctgatt ttcagggatt gcattgtatt ctaacccttc cagaagtccg
     1921 ttgattgtcg taagaggtgt tctcatctcg tgagaagcat ctgccataaa ttgtcttcga
     1981 cgattttctt gttcttcaat ttctgcttga gattctttca atgaattcgt cattttattg
     2041 aaatcatctg ctaactcatc aaattcatct ttgtcatgta cggggagctg tacatcaaaa
     2101 ttaccattcg tcacttcttt cgtcgcattc cttaaccgat tgatacgctt aacttggaat
     2161 gctgcaaaag catagctggc aataactgca acgatactgg aaaaaataaa tcctttgaac
     2221 aggttcagtg taattggacg aacgctgtta tcgatatttc gcgcaggttg actaactaca
     2281 agtgctccat agaattcatg attcaaatta aatggcacaa gtgcgtaaga ggtcgcctgt
     2341 gcttgtccta aaatattttt attcgatgtg aatttttctt gacgtccatt cctcagattt
     2401 ttccattgat cagctgttaa ggtaaaattc agtaaagctg tattagtcgg ataaatgaca
     2461 cgttcgtttt tatcgataaa tataaagttc acattttgtt ctgtcagtac ttgctctgtc
     2521 agcagcaatg cattttgaaa cgcctgatct tgactaaact gcgggagcgt atcggcatag
     2581 gtttgcgttg tcttttcaac tgattcagca tatccaaaca gttgctggta attgttttct
     2641 tcaatcgttt gttttgtcag ttgtgtaaaa gaaactccaa cgatcaataa gatcaacgcg
     2701 accattccgc aaaaagctaa gagttgctga tacaaataac gcattaggca acaccagaat
     2761 catcgaattt gtagccgact ccccaaacag tttggatgac ttgtggtccg accttttcga
     2821 ttttttgtcg taatttcttg atatgggcgt ccactgtccg ttcatcacca aagtactgat
     2881 aatcccagac taattcaagc aactgctccc gagagaatac ttgacgtggt ttttttgcta
     2941 aagtaaacaa tagatcgaac tctttaggcg tcagtccttc aattggccgg ttgtccagat
     3001 acgcttctcg tgttttcgta ttcatcttga aatgactagt ctcgatatca aaatgatcat
     3061 ctgaaactgc tgcttcttct cttgcttctg ccaactcact acggcgatgc aaagctttca
     3121 ttcgagcaat aagcgtcaga ggactgaaag gttttgtgac ataatcatct gcccccatct
     3181 ctaagccgat cacctgatcg ctttcagaat ctctagctgt cagcatgata atcggcacag
     3241 tacttgaaac tttgcggatc tctcttccta ctagcatacc gtccagtgtg ggcaaattca
     3301 ggtctagtgt aatcatatcc caatgttgtg ggttttctaa aaaggcatcg agcccttctt
     3361 ttccatcata cttgaaagtt gcttcccaac cttcatttaa gaaaaacatc tgcatcattt
     3421 cagaaacaga ttgattatct tcaatcatta aaatattcat tatcgttccc tccagtttcc
     3481 aggtcatatt tctgtaattt cttttctgtc ataataaaaa taggcataat tggaaatact
     3541 agcagaagca tttcaatcag aaaataattc gtatgcccag atgtctttaa ataatagccg
     3601 atcaatgcca tcagtaagcc cataagaaaa aaccagtttc cgcttgtctt ttgagccagc
     3661 cgccaatgag cgtctgtttt tgcggcaaga ggcgaatgat acccataaaa aggcagattt
     3721 ccttttgagg gcagaatccg aaaaagactg ccaattatta agaataagat accaatactg
     3781 aaaaaaatca ttctttccct cctaaattct cttcttatag tatactctaa attgttgact
     3841 gaaccaatat gttcaggaat tcttcacgaa aggtttgtct tttgttcatt ctttgttaag
     3901 ataaacttat tccaataaaa aaggagtttt atttgtgtca aattatcgtg caatcacttt
     3961 ctttgatctt gatggaacat tactagatgc gaaatcaaaa atcacacccg aagtggcaaa
     4021 agctatgaac gcattaaaag aaaacaatgt tctccccgtc atcgctacag gacgtacaga
     4081 agcagaaatc cactctatca tggaagatgc gggcattact tctgcggtca ccatgaatgg
     4141 ttctttcatt cgagtagacg gcgaagaaat ctattctcaa acattttctt cagaagaatg
     4201 tcaaaaaatg tatgatcatg tacagttgaa cggtcatgaa ctttcttact ataacgatca
     4261 gaaaatctgg tgtaccggtc atagccaaac aatgatcaat gcttacgact tcattcattc
     4321 agatgttccg gaaattgatc ctttaggctt taaagaaaac acagtaaata tgttgctgat
     4381 cttatctcaa tcaggtgacg aatactatca cgaacatttt cctgagctaa ctttttatcg
     4441 gaatggtcct tactcgatcg atacagtgaa gaaaggaatc tcaaaagggg caggtgtcaa
     4501 acgtctgcaa aaagaacttc agctggaaaa agttcctact tttggatttg gcgatggtcc
     4561 gaatgatttt gccttgttgg aagcttgcga caacaaaatc gctatgggca acgcctatga
     4621 cggattaaaa gaactttcta cctttatcac aaagaaaaat acagaaggcg gaattgtcca
     4681 cgcattgaag tattttgatc tgatttaaga tctttttttg aagctacctc ttttcttaag
     4741 aaaatcgctt attttttctt aagaaagggg cagttattat ataaataatg ggatatgatt
     4801 cgctttttta ttgaatttat cccaatatat gctatactaa ttttgtatga aaaatacacg
     4861 taacccttgt tagctaacgg ggacgttacg gattcgacag gcacagtcga gcttgaattg
     4921 cgtttcgtag gttacgtcta cgtaaaaacg ttacagttaa atataactgc taaaaacgaa
     4981 aacaactctt acgctttagc tgcctaaaaa cagttagcgt agatcctctc ggcatcgccc
     5041 atgtgctcga gtaagggtcc taactttagt gggatacgtt tcaactttcc gtctgtaagt
     5101 tgaaaaagag aacatcagac tagcgataca gaatgcctgt cactcggcaa gctgtaaagt
     5161 gaatccttaa atgagttgac tatgaacgta gatttttaag tggcgatgtg tttggacgcg
     5221 ggttcgactc ccgccgtctc catatatgta tggttgagac agataaacat gtatgaaaaa
     5281 cgttgttaaa tcaacgtttt tttattttgg tcaaggagag aaaagtagag aaatacaaaa
     5341 ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca tttagaaatc cccaatacgt
     5401 attcaggaaa atctatttta aggtttacaa aaagaacttt tgttcgtaca ctctttttga
     5461 ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg gtttaagcat gttccttcaa
     5521 ataagttttt taattataaa ctctagagaa attaattcat cctaacataa aataattgtt
     5581 cactcaaaag tttttctaga acaattaaaa cagcaggctt cacattcgct atacatttgt
     5641 aagaagtaat aattcctgta ctaaaaggca taattgcttt aattattttt tgacttctct
     5701 tcaaacacaa gaagtagttg tgtaataatt gcagaaataa ggattccaat aatagatata
     5761 aaatttgaca tggaaacgtc tataacatta aaagaataaa gtgttgaaga aagtaaactg
     5821 attgaaataa cccctccgta aaaaatagtc tgtgaatata cccacttttt ttcagaagac
     5881 gtacttcgtt tggttctaaa acctatccct gcatttctac tcttatcaaa aaaccaaata
     5941 tttagaagta ataatattgc tattattaag ataaaattac tgtaaaaatc caaaagctat
     6001 cctcctagca actatttaaa tacccaaaaa aattacctct agataatatc atacctacgt
     6061 tttcacttat aaattttttc ataaaaaaag cgtttagagg cataatcgtt aatacagatg
     6121 gtaaccaatg tatctggttt gaactgggat atcaccctct ccatatatgt attactaaga
     6181 ctaatagata ctataaaaga cttgatagtt ttgcattgat tttgcctaat actgagcata
     6241 ggtgtatgct aaacgatact ataaggagtg tgtacataat gaaaagaatt ctgtttatca
     6301 gtttatcaaa tttattgtta cttactgctt gtgggacttc tgcatcaaag aacatacctt
     6361 cagatacgca aatttcgaat agtatcgatt caacaatttc tacaaatgag aaaaattctc
     6421 cggtagaaac ttccgatata aaagtttcta cctctgcaaa aaacagggta aagaaactgt
     6481 cagcacgtct cctttatttc aaacaaactc tgaattagct tcttcatctt cttcagcaaa
     6541 taatgatgat agtttcgcgt ttactcctaa ctaaatgcaa cctttacaaa tgctttacga
     6601 taacaagcct gagctaaatg atcctgatat cagttttgca ttttttgaaa tgattggatc
     6661 ggatgatctt tttaaaacta ctagtttgtc tatccgaaaa taggacagtt ctggcagtat
     6721 aggtttttat cgagtttctc caccaggcct cgttacagag acagattctc taggcgaacc
     6781 ttttgaataa atcaaaaatc atgaacaatc actgccaact aaataaagtg ccttcaaaag
     6841 cttggtcttc tcgcttttga aggcacttta tttaaatctt tatgaaacta aagtatgttc
     6901 atatttgttg ctatagttat atagatattc tgttgtttcc aactcataaa cgtgagtata
     6961 ttttgccact acttttccgc atttttctaa tatttcattg cgatcgacgt catgatattc
     7021 gtctacgcag atcacaacac aattttctaa tttactgatt acttctccaa ccactttttg
     7081 tttcaagcca ataggttggc aaatatattt ttttcctaaa atgatagttt cgttattcat
     7141 atttcatcca tccttaatgt tttttcatta ttcaaaatga taataccatt aaaatatgat
     7201 tgtgaaaatc aataacactt gtgattttat aaaaaaataa aaaaaccgaa aaacagaaat
     7261 gaccactgtc tttccggctc tttttattct tgatttctct tcttttttgt ttcaatagac
     7321 gattttagtt attaataaat gaaaaagcat ttatattaaa atttgctaat aaaagatcta
     7381 catatttttc tagaaatgct tgatcgattt catcataccc tttcgttact ccgcaatcga
     7441 tatctaaaac accgattaaa ttaccttctt ttaccattgg cactacgatt tctgattgtg
     7501 cggctgaatc gcaggaaatg taatttgcat gggttttgac atcatcaacg atcaatgttt
     7561 cttgtttttc agctgattct ccacaaactc cttttcccat cttgatacgt gtgcaagaaa
     7621 ctcgcccttg gaacggtcct aaaatcaatt cattaccatc gaataaataa tatcctgcaa
     7681 agacagtctc aggcaaagtc tcattcaaca atgcagatga atttgctaaa tttgctaacc
     7741 agttatcttc tgcttctaat aatccttgtt gctgtaataa taaaatttcg tatgcatctt
     7801 ttttctgttt ttctgtccac atgataaaaa ccgctccttt ttgatctatg gagaagatta
     7861 tagctcagtt tttttgtaaa cgctaggttt cctcctcaaa aaaacgagat acgtcaggtt
     7921 aatcaccttg aacgcatctc gctgtctttt tctattctgc ttgttggatt tctgctgcta
     7981 aatcagcata atcaatcgtt tcgtgaacaa aagtcggatc atcaactgga aaatgctgta
     8041 caagggacga gatgcggcta atctcaactt ttccttcagc cacttcaaaa tccattacat
     8101 gtccgccaaa tgtatgtgta tcatcgataa aatgcaggtg aaatcccgct gcagcaactc
     8161 cttggaataa ttcaggcgta aagaacccga ctaatgttcc ttggatattg ctttgttgga
     8221 attctggttg agactctgaa atttttgcta gtcttctgta aggtttttct tgcttcggca
     8281 tgatacggat gtgcatattt ttgaataatc cagttatctt cactgccaaa aataggtttt
     8341 ccccggcttc tcttactaat atgtcttttt tcaattgttc tccatctgca gtttttgaga
     8401 cagaaaaact agtatctggc gtaaaatctg taattgctgc atagggggta agttcacttc
     8461 catctaattg ggcaactgag ccatctgatc ttccttgata agctacgcca tctaaaaaga
     8521 tgacttcacc atcaagccca tgcaatgttc ccaatcccag agttcctttt tctaatatat
     8581 gagcaatcgt ttctgttcca tccatcaagc cggccatcaa agctcctaat gtcccgtgct
     8641 ggtataagat tttttcagtc atgtcgacac ttccttaata gaattgatct ggtaagattg
     8701 tttctcccaa tttctcatta tcacggtaat caatcggaat atcaataatt acgggtcctt
     8761 ctgtcgcaaa tccttcttct aaagcttttt ctaattcctc tacacttgta gctcggattc
     8821 ctttagcgcc aaaagcttct gcatatttca caaaatcaac tggaccaaag tctacacctg
     8881 acgcccgctg atatttcatt ttttcttgga actctaccat attgtagtgt ccatcattcc
     8941 aaatcaaatg aacgatattc aattttttac gtaccgccgt ttctaaatcc tgggcagaga
     9001 ataaaaatcc cccatcgccg gaaactgata cgatttgtgt atttggacgc accaatgcag
     9061 cagaaatcgc ccatggcaat gcaacaccaa gcgtttgcat accattactg aataacaaat
     9121 gacgaggttc ataacttcta aagtggcgtg ccatccaaat ataatgacta cctacatcta
     9181 cggtaacagt catatcatct gttacttttg actgaagcgt gttaataact tctaaaggat
     9241 gcaagatacc tgcttctcct tttgattgaa caatgtcccg ctccgttaat tttgcttgaa
     9301 gagatgctaa atattctttt gcatcttctg acacttgttg aggctctaat gaacctgtca
     9361 ataaatcaag agtggctgag atatcaccga tcaattctct ttcaggctgc ataaaaggat
     9421 cgatttcagc tggcgcttca tcgatcacaa tgatccgcgc atctttttcc gcattccagt
     9481 ttcttgcttc atattcgatt ggatcatagc caattgcaat aactagatca cttcgtttca
     9541 aaagcatatc tcctggttgg tttctaaata aacccacacg accaaagaaa tgcgcttcta
     9601 attcacgtga gatcactcca gctgcttgga atgtctctac aacaggcaat gctgtctttt
     9661 caactaattg cctgatggct aacgtttctt tttcacttga tcctcgcata cctactaata
     9721 gtaccggtag ttttgcttct ctaatgcgtt cagctaggta acggatatca tctgcagaag
     9781 cagaacctaa tttaggatcg ctcaatggct tgatgacatt accttggaca ggtgcatcca
     9841 ctacatcttg cgggatactg ataaagctcg cgcctttttt tgaactctta gccatacgat
     9901 aagcattggc aatcacttct gaaattgttt ctggatcttg gatttctgca ctgtattttg
     9961 tgattggctg gaacagtgct gcattatcca tgctttgatg ggtcaacttc aatagatcgc
    10021 ttcgttttac ttgtccggca attgctaaaa ctgggtcacc ttctgctgtc gctgttacca
    10081 aacctgtagc taagttacta gcaccaggac cacttgttgc gataactact cctggctctc
    10141 ctgtaatacg tccgattgct tgtgccataa atgcagcatt ttgttcgtga cgagtcacga
    10201 ttaattcagg accttgatcc tctaattcat taaatacacc atcgattttt gcacctggta
    10261 taccgaatac ataatcaaca tgatgattaa tcaaactttc tactacagat ttcgaacctt
    10321 gtttcatttt ttcagccatt gaacatcgtc ctttttatgc tttattttta attattagtt
    10381 agaaataatc acaaataagt gatacagttt ctaaacctgt ttcatacaaa agtatagcac
    10441 gttttatttc atttgtttag ccatatgttt caaaaaatga aaaagaatga cagtttttac
    10501 ataaaaaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
    10561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
    10621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
    10681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
    10741 ttggtttcga cgctggattt ctcacctgaa aataccagca gttggtgcta aaggaaactg
    10801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
    10861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
    10921 atccctttag ccaaaattgg cgaaacatct ttatgaacaa acgtactggt gaaaaactat
    10981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
    11041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
    11101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
    11161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
    11221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
    11281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
    11341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
    11401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
    11461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
    11521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtaa tattgcttca
    11581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatctg
    11641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
    11701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
    11761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
    11821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
    11881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
    11941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
    12001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
    12061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
    12121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatttttgt aagcgcttat
    12181 ttaacaagtt atgtaaatct ctttattttt ttgatttaaa aatgaaaaaa tacaaaagca
    12241 agactgtcat acgtctcgct tttgtattga aatttcttcg attagagata cttattactg
    12301 ttcaaaagct tctttgaaac ggtgaatcaa ttcattgact acaggtgata aaatccgatt
    12361 ctttttccag actagcatgc agttcgtttc aatcattggg tcaagcggaa cgaatgtcac
    12421 tcgctttgaa tgatgactta cagcaccttc tatgactaaa gcagcaccta cctgattctc
    12481 aattagcgca aatacattga aaatcaaatt gtacgttcct gtgatcagca actgctcctt
    12541 ttttatgcct aaccagtcag caaacattcg ctgtatttct ggacgctttg agatcaaaag
    12601 aggtacattc gcaaagtctt cttttgttat cacgtctttt tttgctaaaa cagaattttc
    12661 taaaatgagc aatccccatt tttcctttct tggtaacaaa atcgtttcgt atttctctgt
    12721 atctactgga tctagcaaaa tcgctaaatc taacaggctt ttttctagtc actccgtgat
    12781 atcatcactt gttccgctgt ggatatgaaa ttgaacgctg ggataatcac ggatcaactt
    12841 ttctagtaat agtgccaatg tattcgaact atcagcttcc acacagccga tcgtaaatcg
    12901 gccgctgaat aattgttttt ttctgttctc gaattcttga gtggtttgat tcgttaaatc
    12961 taaaatttct tctgctcggc ttttttagaa aataacctgc ttctgtcaag atcattttgc
    13021 ggttttcgcg tacaaacagt tctgttccta gttactcttc taactccttc agttgtcggc
    13081 ttaaggttgg ttgtgtaaga tgtagaactt ctgcggcttg agagattgtt ccttcttctg
    13141 caattgtcca aaaatatttt agtaagcgta actccatagc gatgctcctt gattttttta
    13201 cttgatacat tccattttat ttctagataa cacacgatca tatttcttga tttttccttg
    13261 taaacgttcc aaacattctt gcatttcagc aatttttttc gtcaaaagtt cactttcttc
    13321 aatcaaaagc ttacgccgct caaatacagt tgcttctcct tcgctagata ggcgaatata
    13381 ttcaatcaaa gactcgatcg ataatccggc atttcgcata cagatcgtaa agtcgaccca
    13441 ttttaggtct tcttctgtat aatcacggat tccgcctttt gtccgattaa ctggtggaat
    13501 caacccgatt cgttcataat agcgcaatgt atcggccgaa atgccctttt cttcactgac
    13561 ttgtttgata ttcatctgat cactctttct cttaatttct tgccacttta cttatgtatt
    13621 ttatttattc tacaacttta agtgtactcc aagtcaatac tcatttctat taaaactaag
    13681 ctggattatc tatatcggtt gtttgtgtat tatttcaaat aaaaaagtct tgttttttta
    13741 ttcttttttg gatatacttt taacaattta gggaaatttt aggaaaaggg gtataaaaat
    13801 gacaacgtat cagcgaaagg tattagtatc gacatcggcg ggaattgctt tagagaatat
    13861 ggatattatg tttttagctt tttcattatc ttcaatgatt acttcttttc atattagtgg
    13921 tacgcaagcg ggtttcattt caacgatcac taatttgggg atgctcattg gaggtatttt
    13981 ttttggcatc atagctgata aatatggacg tgtgaaagta ttttctcaaa cggtccttct
    14041 gttttcgatt gcttctttat tgatgtattt tgcttcaaac atctacttgg tttatctctt
    14101 tcgctttatc gcaggtgtag gagcgggagg agaatatggg gcgtgtatgt ccttgatttc
    14161 cgaaagtttt tcaaaaaaac aaattggacg agcttcttcg gttgctggta ttggtgcgca
    14221 ggtcggtgcg gctttggctg cgattttagc tgctgtgatc attccaacgt ttggttggaa
    14281 gatgttgtat gtcgtaggtg tactgccggt gttgatggtc ttgttcattc gtcgcggctt
    14341 gactgaacca aaggaattca aatctgtaca atctgccgaa aagaaaacca aacttagcag
    14401 actgtttgaa tcaaaacatc ttgcttggca aacatgtggc cttagtatca tggtaactgt
    14461 ccaaattgcc ggctattttg ggttaatgaa ttggctacct tcaattatgc aaaatcgatt
    14521 agggctttcg gtttctgggt cttcattgtg gatggtcagt actattttag gcatgtcttt
    14581 ggggatgatg acttttggca taatcatgga taaattgggg cctaaactag ccttttcttt
    14641 gtttttaatt tgttcatcct tatctgtcta tctacttgtg ttagcccata gccagtggag
    14701 tttggtagca gccgctgtga tagtcggcta ctttatcaat ggcatgtatg gtggatatgg
    14761 cgcagtgatt agtagtttgt atccaacaga aatccgcgcg acggcgaata atttcgttat
    14821 gaaccttgga cgagctgttg gtggtttttc ttctatcgtc atcggtttct tgatggatca
    14881 ttatagttta actgccgtaa tcttgttttt gagcggtatt tacctagtca gtctgctggt
    14941 cttgacaact cttactggtg ttacagcatt aagggaaagc ttgcagcatt gatagttgag
    15001 tctgtaaatg acaagaaaga ggtgtggaaa aagcttgcat ctgcggtaat aagaccagcc
    15061 aaatcaaaaa catgagacac tgtttccgtt tggctggtcg tattacttga tgcagggcag
    15121 ctttttcaac tgctaatttc cttctatctg gcggcgacta ttctttgacg tccgtcattt
    15181 caaagtactt tactaataac ttagcataac gaaaaactgt ggatacactc gtatattgag
    15241 cgtctgcata atcttccaaa tttttctcag gatgttgata catgaattca atcaaatgat
    15301 aattatatga ttttctgtag aaatgaagat aaataatgta gattgggaag ttctcctgct
    15361 tttccataga aaagcgccga ttattctcat tcttcaaaat gaccttatca ggatattgtt
    15421 ggaacaattc ttctaaatct gttagataat tcaagataga cttacttgat accttcaatt
    15481 cgttttgtag ttcttctatt gaacaccaac gttctgtttc aagcagtaaa tgaatcattt
    15541 taattttctt ttgagaaact tttgttaaaa aagtctgcat attttctcct cctgtatcta
    15601 ctttactata ctacatacaa gaaaaaaacg gatacaaata tcatattgtc acaccgtcaa
    15661 tagtgcataa acctgttatt atttctcttt ttttcctaag tattctattg atatgaatca
    15721 tttaatcgtt tattatataa atgtcaataa tctaataata aaataacgta catattttat
    15781 tattgcatac ttaataacag aactatagca aaaaatcccg tgtatcaaca cgggattaga
    15841 ttaagtttcc tcaatcacta ttcataacac aattattata acaagtaagt aaacaattga
    15901 aaaggacatt tattgaaaac ggttgttttt tacttaagga ggaaatttag tgaaaaaatt
    15961 agttgcgttg tttttggtcg caggaagttt gttgagtggg tgtggtgcta ctacgtctac
    16021 tactcaaagc acaagttcag ctcaggcttc cagcagcagt accacgatct ccgaaagtac
    16081 ggcagattca tcgaaagaac aaattgaaaa gaaaaaagaa gaagagaaac aaaaagcgga
    16141 aaaagctgca ctagaaaaaa aagtgacaga agcggaatct gctttgtcac aagctgaagc
    16201 aaatcctaca aaagaaaact atgcaactgc ttcagcagct atccaagcgc tacctgaaaa
    16261 caaacaagag ttgacaagcc gtcttgctgc aattgattcc acaatcaaag caaaagaagc
    16321 agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa caagcagaac aagcgcaaca
    16381 agaagcaatc gcacagcaac aagcagaaca ggcgaggcaa gaagcaatcg cacagcaaca
    16441 agcagaacag gcgcgacagc aagcagcagc tgaacaggct tctcagcagg cacaagccgc
    16501 agctcctgag caaaataatg aacaaactgt ctatgtcaca ccgaccggtt caaaatacca
    16561 tacacataaa tgtggaaatg gtacgtatac ccctgccact ttaagcgaag ctcaagctcg
    16621 tggtttaacc ccttgctcca aatgttatgg aaactaattc aaaagttata ctataataat
    16681 aaaattcttc tgaaacataa aaaaatagaa gctggcgttc cagatagtaa accaaaaata
    16741 gcagtttaca cgtccatttc gtcagcttct ctcttttttc atttagtctg ttccctctgc
    16801 ttttttggac atcttcattc attctttctc aatcaaatag tacacctctt tttcttttcg
    16861 cagtttatat cttccttttt tcaatttcat tgaaaactgt ttcgaatgtt tatgttttac
    16921 atggatttta agcaggagct cctcatgttg caagcgatag ttcctgcctt ttaaatcaga
    16981 aaatctcact agtccgtatt catcagaagc tttcacaaat ggaacttttt ctctagtaat
    17041 atagtgtttg ccgttgagcg tttttacaga aaagaccatt ccttcgattg gtcgtccgtt
    17101 tgtgttcttt acataaaaag ataaaggata gtttcgctta gcgatggcca aagaatctaa
    17161 ataagcgatg agtagccaaa ctaagccgat tgatccaagt agtataccga taaaccaaat
    17221 aaaaaatttc cattttttcc attgatcaat tccttggatc gctttttttg cctcttttgc
    17281 ttgataagga atacgatgcc ccgtcacaag cagtcgatgt gtattgacca tataaggagt
    17341 gcaggtaagc aaagtcacga gatctctgcc tttttggata cccaaagaat ctacttcgtc
    17401 aggtaagacc acttggattt tttctacttg atacgccaat gtctttccat tgatctggat
    17461 gaaaaactgg tctccttttt tcaacttagg caaatctgta aataattttg cttctgggag
    17521 tccacgatgc ccagaaagaa cggcatgggt gtttttcccg ccagtcggat aagaacttcc
    17581 ttccaataat gacgttcctt tttgcaaaaa tatttccgtt gtctgatcga atataggtaa
    17641 gctgacattg atttttggga tttcgatgac tccaagcata tgttccataa aaaaggcatt
    17701 actaggtaaa tcttttaatg cttttgcatc aactgcttgg ttgaaactgc ctatccctgg
    17761 tgatacgttt tgctctgcca actgttggtt tttctttgtc attttttcct gctgtttttt
    17821 tatttcctct gaattcttat tcgatgcttg cttttgataa tggatgatca tttgctgggc
    17881 aagaaaatca ttcagtgaat cttctacaaa aggataagaa aagatcccga tcccaacgac
    17941 taaaatgatt gccatgaaga tgtccagtat tcttattctg cttttattca tcatgtccac
    18001 ctattttcta atatgtatat gaagaggttg tgaaatcagc gccttgacct gagtattagc
    18061 gaagcaaacg gcaaaatagt tcctcctatt ttgccgtttg ttgagcgaat accaagagac
    18121 cgaccacttg tcacaacctt tctccccatt tgaatcacta tcgtttattt atcgtatgat
    18181 ttttgtttcc aatagtttaa gcttctgttt cttgatggcg ttttttgaaa tagatcactg
    18241 ctcctccaac taaagcagta cctaccaaga tgaatacgat gatccccata ccaccagttg
    18301 aaggcaagat tcctttaggt tggttatcga tcgttgtttc atcagaagtt gttggatcat
    18361 catctactgt aaattcagtg tacgcagctt cattcttagg taatgcgtag ccttctggtg
    18421 cttttgtttc ttgaaggcag tatgcccctt gtttcagccc ttcgattgaa aatttccctt
    18481 ctgaatcaga aatcaatttc acttcttccc agtcagaacc tgtgacttca tctgaccaag
    18541 catatgggta ttctcctgag gcaaggtttg ctattttttt cattaattta acagttggat
    18601 cttttgctct tagaatagaa aattctgcat cggctaattt ttcttgcgtg ttcacatctt
    18661 gtttgatgaa gtggaaatca ccgtagtttt ggacattttc tacagtaatc gtgttatccg
    18721 tagttgtatt ttcccgattg actacaccgt ttggattccc aagattggct tcatctgcgc
    18781 catggatgat accggttgtt tgttgcaatt ttccgttttt tactataaaa gtagtttctt
    18841 tttgtggatt ggttaattga taaccatctg gtgcttttgt ttcaactaaa gtataattgc
    18901 cgtctattaa tccattgaca ctgatggatc cttcagtatc agttgtgaat gtcaacgctg
    18961 actcagcagg atcatagaga gcgacaccag tgttttcatc aaaaccggtc acgaattgat
    19021 tctctgaatt tttgatctgg aattgcgccc ctgctaactt atctacccct gaaaagttcc
    19081 cagctttctg gattttgata ttgctttctt tgatgacatt tttagcatat aggtgaatcg
    19141 tgtctaaagg tttgtcaggg tctagggtaa gcttaacacg atatttggcg acgtttcgac
    19201 accgggcttt ggtgtttcta caaagacgta tacagcatct ttcccatcag atttacctgg
    19261 aagagtcagg ttagctaatc cttcaccaga agtcgttaca cttgcgaagg gtgtagcgtc
    19321 tgctggaact gctgtgtctt tacttggata gtacgtagca gtgatgtcat atacgttgaa
    19381 ttcaacgccg ttcaacggtt cgctattcgg aaatgtcaat tccgttccgt tgtttatttg
    19441 attttctggt gctgaagtga atttcaattt atgcaaatta accgtaacat tttctggtgt
    19501 aggtgatacg gctgcagaaa cctctgcagt tcctaggccg agaacgaaag gaagcagcat
    19561 gatagcagtt cctaaaaacg tccatatttt tgttttgatg tttttcattt tatttccctc
    19621 ctattgtttc ctttttgttg ttttttataa aaccaagctg tccctgttga aaggactagc
    19681 aataacccta aaattgagaa acctccaagc atctcttcgc ttgtttttgg aaagcttcct
    19741 ccattttttt catttgatgg accatttgtc tgcatctgaa ctggcgtttt tccattattc
    19801 gtcggatttg cttttggttc gttgatgatt tccaatggta taggatgaag cggatcataa
    19861 ctgccttttt caacttcaaa gagaatggtt gattgactca atatatatcc tttaggcggt
    19921 ttaatctcca ctagttcgta acttccaaaa gacaatccat ggacagaaaa tgttccatct
    19981 ttactggaag taaaaatcgt tgctcttgaa tcatctttgg ctacccaatg atttatcccg
    20041 ttttcttgcg ctaagtattg gtcatttttt ttgactagaa attgagcatc tgctagtttt
    20101 tgtttcgttt gccgatccac tttgacaaat tgtttgcctc ctgtttggat ggcaagatgt
    20161 gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt tatcaaatgt cgttttttca
    20221 ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt tttttccagc aaacgaacta
    20281 agatcttttc cagaatctga taagacaaaa ccatgtttta ttttttgtgt gtgaaatgta
    20341 tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg gttctagcca taactgagga
    20401 tcagctgtat ctttgatcgt ataggttcct atctcgttga tgttctcagg aattgttacg
    20461 gtgatgagat aagggacttt atccccatct gcaaaatctg tctgcttatt ctctactgtt
    20521 tttgttaatg tgagtgcagc aggtattttt tgttcgctct ttggatacaa gtggatggtc
    20581 gtcaatttat gattagatga atctgtcaca ggaagcacaa cgacaaatgg tgctgatttt
    20641 tcttttactt aatctgaagt ctttatttcc gtaaatcggt aaacagcgtc acgaccttgc
    20701 tcatctttgt ccgatgcact aaaagaagca atgccctctt cctcgtctac tgtttttgtc
    20761 acattttctg ccagtatttt ttcactatca cttttttgtg ctagttttct ttgtgcgtct
    20821 tccacactac ttccttctga ccgcagcttg taaaattcat ccgagacatc atatatagcg
    20881 aaagtaacgc cgttcaaccc ttttactcct tgaaagttat ccgggatatt tattccgtcg
    20941 ttttgactga tacttggcat cttatcgctt tcgaacattc gattatgcaa agtaaatgta
    21001 ataggtgatt ctctagctga cacagtcgga gaacctataa aaaagaccag taggataaaa
    21061 aagagaacag caaatatatc cacacgtaca ttttttgttt ttttcatcgc tgttcacctg
    21121 cttttttctt acgatggata aaataaaaga agccagaaaa aataaagatc agtcctaaaa
    21181 agacaaaggg cagtagacct atcccacctg tcgaaggaag caagagagtc ggattgatcg
    21241 tcaacttagc ggtatctgtt gcgataatcg tataattatt gtttacactt ccttcgccta
    21301 aactaatcgt tactatttgt gatccaatat ctgtccggct gttaactgtt agggtataat
    21361 ccaatgattc gcctaagaca acaccggtaa cgtggagatt tccaaccgaa atcgagtgac
    21421 tttgggcatc ataattaaag gcagcgtcat cgacagtaat cactgcgtta gctttataga
    21481 tggtataagt tcctgatgtg ttggctatat cagtaaatga caacttggga tttgctgctt
    21541 tgactttgtc tattccgcta ttcgttagtt taattgtata gctgccagca ttaaccatat
    21601 tttttgctga ttgtgcatct gctgtgtaat ccatactgct aagtgccact tgtactggag
    21661 taagcgaact agtgacaccc tctgctagct tgacatggat tgacgggaca cctgcttttc
    21721 catcatatgt cttactgcca ctgagctcta ccgcaccgct gatttgctta atcgtataga
    21781 catcagatgt ctgcacgata gtaaataaat agtttgttct ggcagctagt ttagctagtc
    21841 cgctttttgt caattgccaa gacgtatagg ttccaatttc agtaggcgca gttacttcaa
    21901 ctcccttttc attcatcgct ttgaaatcag ttggattcca tgtgatttga tcaatactga
    21961 gatcattgtt taaaagggat gttgattctg ttttatcatt aaaggcatat ttaacgatcg
    22021 gtacaacaga aagaccatca tagtacttac tgccatttgc attgattgat actgttgcat
    22081 ttgtcttata atataggatg acttcagaaa tctgttttcc atttgcgtcc tttacgatta
    22141 catttccatt tgtatctaaa ctagctaaaa cactcattgc tgattggttc tttgtttcta
    22201 tcgctgaaac agctgtaaag gtatatccgt tgatagtagg cgtattagtc gtcaggtctt
    22261 gtgcagcact tccgtaagta agattcatta ttttagaagg agcaatcatc tgtccaaaat
    22321 agtcttgata cttaatagtg attggtgttt gtttgcctgt ataggtcacc gtatcgacgt
    22381 tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa agaatagccc gttactgctg
    22441 ttggagtgat aaccactttg tcacccacat ggattccaga taatgtagtc ccgtacgttc
    22501 cgatttttgc tgctccacca ttgactgtat aagaaaaagt tcctgtccga ttcgcattga
    22561 cctgaaccgt tgctgcttga taggttggaa aatttgaaac atttccctta ccatcaacga
    22621 tctgcccatt tttgatcgag taccccgtga atggaatatc atttgctaac tgtataccga
    22681 ttgaaaaagt actcatacct cctccggcag gaagccctcc ccatgttgag ggtgtaaaag
    22741 aataactttt atccgcattg ggtgtaactg aatcgatcgt atttccagcg gtagccaccc
    22801 ccatatagat actttttggg aatgtgatag tagtaccatt tactgtggag ccaataaaca
    22861 gattatggat atctggacgg ttatcaataa aagcgattgc tttggttacg ccttgtaacg
    22921 cagttttatc cgcactcatc gatccgttaa ccgcaacaat gactccatct ttggcatata
    22981 tcactgtata tccatctggt atggtatgtc cgttgaaatt cgcagtggcc gcaacagtac
    23041 taaatgtctg gcttctacct gtttctgaat aagctttctc ccctgctgct tttgctgctg
    23101 tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga tgtggttgag gttgtctcgt
    23161 tgttgctaga ctgattggtg ctttctatgg actgtatttc agaatagggt ggactatttg
    23221 tctcctctgt cgtttggtta gcaaaatttg acgtttctgt ataaacgttt acataaatac
    23281 ttgataagct ccctaagaaa gtaaaaatca agatgatcga aatcataatg ggatgtaagt
    23341 attttctttt agatacagct ttgagtctag aaacaaaatg tttttgacat atacgttgga
    23401 ttttattttg agttttctta atcattttca tccctcctct caataaaaaa tcatcaaata
    23461 atcctttaca cgttattcta aatttaattt ttattatttt attgatgtat agaaagtttg
    23521 gggctttttt tctcttttta ttctaaggtt attttagtct tgtcctttaa tacaaagatt
    23581 ttatttttaa ataaaaaata ctcttttttt acgattattg aaaaaaaaag ataaaacttg
    23641 attaattgaa gtattgctta aatttggaag ctatgaggga agaataaaag agttaggacg
    23701 ggattatcta ctatttttct ttgttttaaa tatttgttat ctttgaacat aaaagagaaa
    23761 tgtgtcgact gcagtgttgt gtccct
//
LOCUS       DACDBG010000039        23349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDBG010000039 DACDBG010000000
VERSION     DACDBG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.039"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(335..1627)
                     /locus_tag="IVZ90_001532"
     CDS             complement(335..1627)
                     /locus_tag="IVZ90_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8262588.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1624..2247)
                     /locus_tag="IVZ90_001533"
     CDS             complement(1624..2247)
                     /locus_tag="IVZ90_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262589.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2250..3086)
                     /locus_tag="IVZ90_001534"
     CDS             complement(2250..3086)
                     /locus_tag="IVZ90_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262590.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3103..3990)
                     /locus_tag="IVZ90_001535"
     CDS             complement(3103..3990)
                     /locus_tag="IVZ90_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8262591.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3998..5233)
                     /locus_tag="IVZ90_001536"
                     /pseudo
     CDS             complement(3998..5233)
                     /locus_tag="IVZ90_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5916..6599)
                     /locus_tag="IVZ90_001537"
     CDS             complement(5916..6599)
                     /locus_tag="IVZ90_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8262592.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6617..7234)
                     /locus_tag="IVZ90_001538"
     CDS             complement(6617..7234)
                     /locus_tag="IVZ90_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8262593.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7244..8068)
                     /locus_tag="IVZ90_001539"
     CDS             complement(7244..8068)
                     /locus_tag="IVZ90_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8262594.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8061..8621)
                     /locus_tag="IVZ90_001540"
     CDS             complement(8061..8621)
                     /locus_tag="IVZ90_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262595.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8795..9751
                     /locus_tag="IVZ90_001541"
     CDS             8795..9751
                     /locus_tag="IVZ90_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8262596.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10056..11012)
                     /locus_tag="IVZ90_001542"
     CDS             complement(10056..11012)
                     /locus_tag="IVZ90_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8262597.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11116..11718
                     /locus_tag="IVZ90_001543"
     CDS             11116..11718
                     /locus_tag="IVZ90_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8262598.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11806..13305)
                     /locus_tag="IVZ90_001544"
     CDS             complement(11806..13305)
                     /locus_tag="IVZ90_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8262599.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13321..13845)
                     /locus_tag="IVZ90_001545"
     CDS             complement(13321..13845)
                     /locus_tag="IVZ90_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262600.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14143..14892
                     /locus_tag="IVZ90_001546"
     CDS             14143..14892
                     /locus_tag="IVZ90_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8262601.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14883..15668
                     /locus_tag="IVZ90_001547"
     CDS             14883..15668
                     /locus_tag="IVZ90_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8262602.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15637..16353
                     /locus_tag="IVZ90_001548"
     CDS             15637..16353
                     /locus_tag="IVZ90_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8262603.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16455..17474)
                     /locus_tag="IVZ90_001549"
     CDS             complement(16455..17474)
                     /locus_tag="IVZ90_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8262604.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17493..18551)
                     /locus_tag="IVZ90_001550"
     CDS             complement(17493..18551)
                     /locus_tag="IVZ90_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8262605.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(18771..19919)
                     /gene="nagA"
                     /locus_tag="IVZ90_001551"
     CDS             complement(18771..19919)
                     /gene="nagA"
                     /locus_tag="IVZ90_001551"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8262606.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19995..20831)
                     /locus_tag="IVZ90_001552"
     CDS             complement(19995..20831)
                     /locus_tag="IVZ90_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262607.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(21015..22232)
                     /locus_tag="IVZ90_001553"
     CDS             complement(21015..22232)
                     /locus_tag="IVZ90_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8262608.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            22615..22698
                     /locus_tag="IVZ90_001554"
     tRNA            22615..22698
                     /locus_tag="IVZ90_001554"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22649..22651,aa:Leu,seq:gag)
     gene            complement(22938..>23349)
                     /locus_tag="IVZ90_001555"
     CDS             complement(22938..>23349)
                     /locus_tag="IVZ90_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262609.1"
                     /translation="RAKIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIV
                     GIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAV
                     KELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         7002 a         4902 c         4029 g         7416 t
ORIGIN      
        1 actgttttgc acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt
       61 gaaaacagtc taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa
      121 atctaatgat ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt
      181 tctcctatct aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc
      241 ccctttttcc gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta
      301 taaactacgg gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt
      361 caatcactac tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg
      421 acaaaatacc atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac
      481 caaatagatc atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga
      541 cgattacctc atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg
      601 ctccttcatc ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt
      661 cgatccgtcc tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt
      721 ttagcgaata cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt
      781 caatagagat atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg
      841 cttctaaata catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag
      901 ctttagctag ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg
      961 cattatgcat cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt
     1021 tcaaccaaaa attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc
     1081 tattgtgctt atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca
     1141 gccattcgaa gtggatagaa aacacatcac caatcacatc actttctatc agttccttta
     1201 tcttcgtgtg atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct
     1261 gtttttggac cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc
     1321 aaataacatc atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta
     1381 ctgacgtaat aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg
     1441 gcatacatgt ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat
     1501 ccatccttgt ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag
     1561 aggcaattgc ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt
     1621 tcttcattta aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc
     1681 tggatacagt aatgcagtga gtaaaaaatc aggctgtata caccagaaca gtccatacaa
     1741 aaggcataaa taccacagaa accccttgac gttttttgac aacagccaga tattcctttt
     1801 gatcaaataa acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa
     1861 aaaactatgg gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc
     1921 aatctttccc actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa
     1981 cgtaatgatg aaacacatcc gtttgatttt ccctttcctc caacatacct ttttttctgc
     2041 caagtaatgg acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac
     2101 aacacgagca gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag
     2161 agcattactg ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag
     2221 aaacatctct tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc
     2281 tggacaagat atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta
     2341 gacatcgcaa acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg
     2401 gcaatcgata ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg
     2461 tcccatttcc aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga
     2521 aaaataattt tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct
     2581 aactctttgg ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca
     2641 aaaaaatgtg gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata
     2701 ataaactgcg gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg
     2761 aaccagaatc tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag
     2821 ctcagaactg tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta
     2881 aaaaatgaaa ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc
     2941 tctggaatca aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg
     3001 atcatccata agaccggata aatcatgact agacctaata gcaaaataaa accgtattga
     3061 aaccatttcg ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt
     3121 ttttgatgat ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga
     3181 aatagcacta gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc
     3241 ataaaagttc gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt
     3301 cataaacgcc gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg
     3361 taacgtgatc ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata
     3421 atacgagcct gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg
     3481 ccaagcagac atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag
     3541 cgcaggtgcg cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc
     3601 ccgccaaact agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga
     3661 acgaaagaaa gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa
     3721 gatcagtttc acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa
     3781 aaaacgggca tcactcagca tctttatata attatccaag ccgatccatg agactggatt
     3841 tcccaaacga taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat
     3901 caatagaaat cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc
     3961 caagaaccgt tttagttttt cttttttcat gccttccttc tgctgttact tcttcataaa
     4021 tgactttttc aactaaaaga ttgtattggt cttgaatttc cggattcaaa gcactatcaa
     4081 agacattcac ttcgccgttg tccatatcgg aaatcatttc taaataatca aatgtcgctt
     4141 gcaaagttgc atcatctgca cgagcgagct gttctcgaat attcgacatg attggtacac
     4201 cacgttctcc ttttaaaatt tcattggctt cttcactgtt ttgaaagaaa tcaataaact
     4261 tagcagcttc ttctttatgg gctgagcttt ttgccatact cagcatttgc gaagaacgta
     4321 aagggatacc agattctcca tctgcttttc tgcgaggtgg attaatcaac tgaatctcca
     4381 tatcttcagg agcccctgac aatatttctg tcaactggtt acttgataga aacaccattg
     4441 ccgcttctct atccctaacc gcctgaaaag acagatccgt cgtacttgca gctccaccac
     4501 ctggttctgg atgggcccct ttgtctacca gatttttacg cagctggaaa aaatctgtta
     4561 gatagtctgg attttcaaat cccaagctgt tagatagatc ttcccgatta taaaaattca
     4621 atcctttctc aacttgtggt atttgaataa tacagccagc tataaaatct tcgaatcttg
     4681 aaatagcata gattcctaat tcttcagtga tttgatagct gatttgttca aattcatccc
     4741 atgtccaatt tgaagctggt tcagggatat ttaattgttc aaatatattt ttattgtaag
     4801 caatcgcaaa cgaattcacc ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg
     4861 ttgctaaata ggattcatct gcattagaaa tatcaattat cccttgctcc acatattcat
     4921 ctaatggttc aatctgattg atgtattgag gaaattgatt gttcagttgg aaaatgtccc
     4981 agatatcatc tgatgctgct agtgtaccta attttgtgta atatccttca taatcataaa
     5041 attcataatc gatcgtcaca tggggatttt tttcttcata aagctcgatt acttttactg
     5101 ttgcatcgtg tcggacttgc gaaccccacc aagccatgtt cagcgtgatt tttttgtcat
     5161 cttcttgccc acaagccgtc aagaagaata acataacact taaagctaag atgattctca
     5221 ttcttttctt cattctctgc acctctactt ccattgttat ttttcaatat actgtatttt
     5281 tatacagaac atatttatta aacgatcttt ttcaagggga ataaaaacca agacttgcct
     5341 tatcgttctg cctaggatga acaactcaat actttcaaga aaaattagat agtgctgatt
     5401 cttctttcat cgctaaaaac acttcttgct tcaatgatgg gaggataatt acgcaaagaa
     5461 aaaaggatat cagacaactg cccaatatcc ttgacatagc taaataagca tgcttcttcg
     5521 tgtgcgctct tccctaaaaa acagaaaaat aactagtaat tctgtatgaa attaggtaca
     5581 atatacacgt cgatgcacaa gcattgacta tggcaacttg attggtcagt tcccgctgac
     5641 ttttcaagcc ccgacaattt agctgctact aaattgtcgg gtgccttttt tagtttttat
     5701 tcgtttttca ttctatagaa agcgcttcaa taaatcaaga catttttttg ttagaatgct
     5761 aataataaca aaaggtttta actcttctta tgtacattac aggtaaaaaa acaaaaaaat
     5821 ttctcttttt tttgaattct gaacaaatag tactggagta caaaagacga ggaagagcca
     5881 ctatctgctc ttcctcgtct tttgattctc acctgttatt catgattctt tttctcagtt
     5941 tcccgatatt tttccatttt acgactaaaa agctctccgc tacttggtgg cgtatatgtg
     6001 atggcatttg ctcctgcttg aatggtctca gcaatacttt cttccgttgg accacctgtc
     6061 gcaatgatag gaagttcagg atatttttta cgaaaatggc gtactgcttc tgcagtttct
     6121 ttacctgcac tgatattgat aatcgaaata ccagctgcta acttttcatc tatttctgta
     6181 tatttcgacg ttaccgtact gataactgga atatcaacta ctcgacaaac ttcttttact
     6241 gtctctaaag gcgtaggacc gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac
     6301 atgctcatgt aggaagaacg agcaccttgt gtcaaaccac cacctacacc ggagaaaact
     6361 ggaatgtcag ctgcctcaat aatgctttta gtaatcgctg gatgaggtgt aaaaggatac
     6421 acagcaatta ctgcattcgc atttgtatta cgtatgatcg caatatccgt agtaaagatg
     6481 atcgaacgga tcaattttcc aaatatcttg atcccgcttg cttctaaaat gatttccggt
     6541 actcttacga tatcttttct caattccgtc atgatttctg gcgtaaattg ttctgccatc
     6601 agccacttct cctttgctat ctccggtcat actgattcaa ttgatacgtc tcgatgacct
     6661 ggatgatttt ttgcgcataa ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg
     6721 cggcttcctg atagttggtg gccgtaatca ctttttcata cagcgcagga ttccaatcta
     6781 ctccttggac gaacagttgt gtatggtcat ccatggactg ttcccatgaa tcatacactt
     6841 tgaagtctcc ttggatagtt atccattctt cgttaacaaa ttcctttgtt tccaggctta
     6901 ctttcttctg atccccataa gccttgatcc cgaaaagatt attgtatttg ctagccaagg
     6961 tactttttcc ccaatttgat tctaatatag cttgtcctaa aatgatacta ggaagcactc
     7021 cataaccatc ttgtagttct tgggcatgcg gaacaagccg ttcaataaat tgctgatggg
     7081 attgcgcttc attttcttgc tgattatcag taaaaggaga agacagattc ttcaaggaaa
     7141 aaacaaaggc taagagtatc actgctacac cggcaaccac tgctggccaa cggatattct
     7201 tgcgcttttg ctttttccac ttttttttcg ccatgatcaa ttcctacttt tcatttaatt
     7261 cattcagata ttcttctagc gtcaattggt gtttggtgat atactcagcg ttttctttac
     7321 cgacataacg taagtgccaa ggttcatatg tgatcttagt tatatcttcg tgtccttttg
     7381 gataacgaac gataaatcca tactgcgcag cattatctgc gatccattta gctccaggct
     7441 gatctccgta acttgcatcc aatacagtag aaggataact gttgtaccag ttttgatcga
     7501 cgacatcgac tgccagacct gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt
     7561 tagtaatctt tgtcgcctcc tcttctgata caccttgacg actcatcaca tcttggacat
     7621 tttgttcgaa aacttgttgt tgggatgata cagaccggta agctgagacc atcactaatg
     7681 gaaatccagc tgcttcagca gccttggcaa atgcttcata ttctggagca atgcgtttgt
     7741 cgatcaagta tccgttactt aatgatgcca attgagtaga ctcatcaatt tctttttcta
     7801 atttgtgatc tggaccaacc aaaactagct tccaatcgtc tgttttaaca tctggcaatt
     7861 gtttggacgt acgatctttt gtttctttgt cctttacctt gctgctggtt gtttgactgt
     7921 tcttttcttt actagcagtt gttttggtgc ttgttcgttc gactgcttga ttgccggctt
     7981 gaacatgcga atcatcatcg ctaaaaataa catagcccat tgctgcgatc ataatcacaa
     8041 ctgctccaaa tcctaaaaat ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc
     8101 ccattcgcct catgaacgta agtttcttcc agattttcat tcacccaatc gagaagttcg
     8161 attttttctc cttcgatctg tttttgaaca tctgctggca gacgaaaatt caaaatatca
     8221 tcatagcccc attcatcata ttgaatactt actttcaagt caaggtatct accagcctgc
     8281 tgattatttt tcaaagggat cagctcgact tttgctgctt tgctgctaag ccatttttgc
     8341 gcgatgcgtg ttttgatttg cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa
     8401 ataatcgttt gacgcaaaat cttttgcgtt agcatccatt cataatcagt gaaaagattt
     8461 tttactttct gcatcgcttc agatggcaga tttttctcgc catctttcca tttttgccat
     8521 tgttctggac tgatcagcaa gtattcatct aaaaaatttt tttcagtttc aaactgttct
     8581 aataatacac tcataataag atcaatatat acttgatgca cgtttatccc ctccttttaa
     8641 ctattctact taagttttga tcaaaaagca cctcttaaat cgctgctttc atttttttta
     8701 atgtttcaag tcctctcctc tttattcttc gtgatttttg ttataataat agacagacaa
     8761 gaaactcaat ccaactatga aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt
     8821 tggtcttggt accatcgcac atgagtttgc cgaaagcttc aatcaagaga cgagcgaact
     8881 aacagcagtt gcttccagaa cttcagaaaa agcggaaaac ttcgctcacc gttacaatat
     8941 tccaaaagct tacggaagct atcaagaaat gttagacgat gctgaaatcg acatcgtgta
     9001 cattgctgta ccaaatagac agcatatcga ccatatcttg gcagcattgg aagcaggcaa
     9061 acacgttcta tgtgaaaaag ccatcacgat gaataaaaaa gaactggctg acgctatgcg
     9121 cttagcagaa gaaaagaatt tgatcttagc agaagcgatg acaatcttca acatgccact
     9181 ttatcagcag ttacgctcaa taatggatac aggaaaactg ggggctttaa aaatgatcca
     9241 agcacctttc ggcagttaca aagaaccaga ccctaaaaac cggttcttca atccagaatt
     9301 agctggagga gctctgttgg atatcggtac gtatgctgta tctttcgctc gtttcttttt
     9361 gagctctcaa cctgaagtag ttgcctctac aatggttcct tttgaaacag gcgtggacga
     9421 acaatccgtc actatcttgc gtaacaaaga aaatgaactt gctgctgtca gcttgacttt
     9481 tcaggcaaaa atgccaaaag tcggtatcgt tgcctttgag aacggttatg tgacgattgc
     9541 tgattacccg cgtgctgatc gtgcggagat cttatttaca gatggtacaa aggaattcat
     9601 cgaaagcggg aatacatctg aagcaatgaa ttacgaaata aagaatatgg tacacatgat
     9661 tgaaggtgac ttgcccaatc gctctctctt cttgacaaaa gatgtcatcg aaatcttgga
     9721 tcaaatgcaa gttctatgga aaaatatgta attttaaaag gttgtgaaaa agctgttctg
     9781 catcaagtaa taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggctggac
     9841 ttattaccgc agaggaaagc ttttgaacac cgtttaagaa agaggttgtg agcctaccgt
     9901 tcagctccga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg
     9961 tgacttgtga ccgaggagtt gacaggagaa caccgtttaa agaagaggtt gtgaaatcag
    10021 ctttttgacc tgaatattag ctgaattcac aacctctatt tttggatttt ttgttgggag
    10081 aattctgcct tgatttcaat aaaactttta aaagactgca aaaactggaa caattctgct
    10141 cgatcctcga attcagggcg actagtcggc agaggcattt gccgatacaa tacataaact
    10201 tcttcaatac gtgttaaaat ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta
    10261 taaatcaata aaccatagat atgttttcca taataagctg gttgctggat tctctctaga
    10321 ttttccagca tgtctttaat cacattggcc tgtgctcttc tcatagagaa gtaagtttcg
    10381 tagtatagcg gctgtctgag ccagtagttt tcttgatgtt ctctggcatc ttcttggctt
    10441 tcgcgaatga atgtcagtaa atgttcgcat ttgatctgca catcttcttt attttctgat
    10501 aaaagaaatt ctgccatttg tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg
    10561 ttttccttca gctgcttcgt acgatctggc atgtaaacat ttgcaagcaa agcaaatact
    10621 aatccaatcg ccattaatcc cgcttcattg ccgatcagct gccaagacat gttttcctcc
    10681 actaaataat gagtgaccaa aaccgaattt actactatcc cttcggttaa tttgaagcga
    10741 gaggcaagcg ggatgaacag gaataaaaaa agactgaagc caaaaatagt atacccaaca
    10801 gcaaaaaaac tgaacagtgc tgttgccgta gctaatataa aggctgcgat tcgattcatg
    10861 cccgtacgca atgtagagcg cttggtattt cctacactta ataatgcaat gatccctgca
    10921 gctggccaat acaacagcga taagctaccc gctgtgacca tcgaaaagaa gacactgatc
    10981 actgttttga ctgttcgcat tcctatacgc atttacttcc ccctttttta ttttaattat
    11041 acttgaataa tgaaaaacta gctatcagac aaaagtttag gtatgatagt aaaaagacca
    11101 aaaaggagaa tctctatgca atttggccgt tttcgactag gtatgcgtac gatcaaaagt
    11161 gccttagctg tttttttatg tatcttgttt tttcatttga ctcaacgagg aatcccgatg
    11221 attgctgcat tatctgctgt tttttctttg cgtcaggact tgactacaac cgtttctttt
    11281 ggaaagtcta ggatcatcgg caatacatta ggtggtacat tagctatcat ttattttttt
    11341 gtcaaaagct attttcacaa tgattttttg gtagaattga tcttgcttcc tattctagtt
    11401 attattgtga tcgtcgtgtc agatggtatt aacaataatt ccggtatcat ttccgcaata
    11461 gctacgttgt tgctgattgc gttgagcatc cctcaaggcg aatccgcttt gtatgctatc
    11521 caacgtgtac tagatacttt tatagggaca ttcattgcaa tcggtatcaa tttctttctt
    11581 cgtcctcctg aaacagaaaa gaaggaagaa ctatcagaag atttggtcga actccaaaag
    11641 aaagaacgat tgctaagaac aaatttacaa gagatagaag ctaagatcca aaaacaaaag
    11701 aaaaatagcg aggactaatt ttcttttgca ataaaataaa aaaatggaga gaagaacttt
    11761 aaaaagtctt cctccatttt tgattgaacg gtgaagtgta ctactttatt cttccttcat
    11821 cgagttttgt tctgcctctt ttggcgcttc atacatattt acttggctag tagtcccacg
    11881 tgtcgacaaa ttctctacga tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc
    11941 ttgagtagaa gaaagcaaat ttgtggcata atttgtcact cggttcgctc cactggtttc
    12001 tcctgaactg cttcctgtat cgacaacaga gattttttcg atatttccta gcggctgtgc
    12061 tgcttcgcgc atcaattgcg gtagcatatc gataaccata ctcaagacag ctgcttcgcc
    12121 atattccttg aaggcattgg cgatcttttg ttttgcttcg gcttctgctt ttccttttgc
    12181 tagtgctgct tcggcttctg cttgtccagc taaacgtgtc ttattggctt ccgcttctgc
    12241 cagtgcttcc acacggaatc gttctgcttc ggcttcagtc acttctttga ctttttgagc
    12301 ttgtgcttct tgttctttag cgtaccgatc ggcatctgct tttttcttca cttccgaatc
    12361 atattgcttt tcacgacgtg tgatttcttt ttcttccagt tcgatttgtt tttggcgttc
    12421 tacgatttta atctgcattt cctgttcgat tacttgttgt tgtgctcggg cactttctaa
    12481 attgtaggct tggtctgctt ttgctttagc aacatcttgt tcttgtttgt aggttgctaa
    12541 tttcagttct ttttctttta atgattctgc gatctctgtt tgtcgctgta attcagctgc
    12601 ttgtgattct ttttctgctt gagctttttt gatacgcgtt tcttttagag cttccgcttc
    12661 tgctatatct gcatctcttt tgacttgggc gattcttggt ttccctaaag agtcaagata
    12721 accgtttttg tctctgacct ctttgatagt gaaagaaacg atgatcaacc ccatttttgc
    12781 aaggtctaca gaagcgactt cttgtacgct ttgactgaac ttatcgcgat tttgatagat
    12841 ttcttctaca gtcatagaac caagaatcga gcgcaagtgt ccttctaata cttctcttgc
    12901 ttcattttcc agttcttctc ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc
    12961 ttctacagat gaaccgattt tgatgatcga agtaccatca cacatcaccg gtacgccttg
    13021 ttcggtatac acttcagggg tggagacgtc caatttactc gataatagac tgatccgatt
    13081 cgaacgttga aaaactggca agacgaatgc gccacctcca cgcacaattt tgattttgtt
    13141 attgctttca tcaatgtgca catttttgct tcctaaataa cttccactga tgatcaacgc
    13201 ttcatccggt tttgctgtct ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat
    13261 caaagctgcg attgcgatcg cgataaaaac aactggtatt tccatttaac acacgctcct
    13321 ttaagctttt atcatgcttt cataaggcat gacataacag attctttcct tgatctcaat
    13381 gatcaatact ttcgttcccc gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg
    13441 acgactaatg gaaccggtaa cacttttcaa ctgaatttct cccatacctc gtacagggat
    13501 cggtgtgatc acaacagcta cttgtccttc cagagttttt tcagaagacg acatcgttga
    13561 ttcggcatgt ttcatcggca tcaatatata gaaattaagc aaaaatacca acactatcga
    13621 tatgcctcca ctagcaataa ataaaaataa agatgattga tctgttaaac gctcacctag
    13681 aaaaccaaat aagcttgtga aagttaacca agggataata agcatcggat caagcgggcc
    13741 atcaaaatcg aatacatctc caaaaataat caataggact gccaatcctg cacaaatcag
    13801 caatgtatac caataaatcg tctcaagtgc gattccccct atcatataca cgcctccttc
    13861 catttctacg tctatcatac caatgtttga aaattctgta tagcttcttt taccgactca
    13921 gtggtgtttt ttataggaag gttgtgttta tcgttgacaa tttgttaaat ttttgttttc
    13981 atgttaaaat tttcacaata aattctttcg aggtagccta tgtttccttt ttttaagaaa
    14041 aaaccattaa agctccagaa cacgatcatc cttgtcgttt gtttctccac attcgtttct
    14101 ttgattgttt ctgccatatt gatcaggaat tttattcttt cgatgaaccg atctagtgtg
    14161 tcagataaaa tctctagcat tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag
    14221 ctgcagactg gtgacccgac acatgcgatt caaggaataa caaatgaaat catgattgca
    14281 tcgaatttag attttatcgt tgtcatggac cctgaaggca ttcgatattc tcacgcagac
    14341 ccaagttaca tcggtcttcc tttctcaagt catgaagatg tgaaaaaagt gattggagga
    14401 caagaatttt tctctgaaaa agaaggaatg ttagggttcg gcttccgcta tttcgtccct
    14461 atttattatg atcaagagat tattggtgca gtttgtgttg gcttaacaaa caacacgatt
    14521 gacaatcaag tcaaagatgg acaaaaaatg attgtttttt cattgattat tggacttagc
    14581 gtaggcatca taggcgccgt ttttcttgcc caaaaaatga aaaaagtact gcttggactt
    14641 gaaccacaag aaatagcaga acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat
    14701 gaaggaatct tagccatctc ttctgataaa actatcctct tgatcaatac acctgcaaaa
    14761 aacattttct accaaacaga gcagctattg gatttcgaag taggaaaaaa atcagtgata
    14821 atctatatga cgttttgttt aaagagacga ttgagcatca aacgaccgtt cggactcaat
    14881 ccatgtattt aaatgggata gaaactattt ctagcgttac acctcttttt agcaatgacc
    14941 gttttttagg agcagtcgca actttacgaa atcgatcaga agtaatgaaa ttaatggagg
    15001 agcttggggg gacgaaacaa tatataaact ccatgagagc tcaaacccat gaattcatga
    15061 ataaacttca tgtcatttct ggtttgattg atttgaagaa gtatgaagaa gtcacccatt
    15121 ttatccagca gctaaatcaa aattataaaa aagagattgg ccaagtctca agtctgatca
    15181 aagtacctgc aattgccggt tttttattag gcaaaataaa cgaagccaaa gaacaatctg
    15241 ttgaaatgct gttagaaagc gattctttta tccctaaaat ggagatggat gactccatcc
    15301 attcactttt gcaagtgtta gggaacttac ttgataatgc aaaagaagcc gtgctacaaa
    15361 aacatgaaag aaacggacag gtccggattc atttaaacta tgacacagaa gctgaagtat
    15421 tcatcgtgac cgtctgcgat ggcggtgtcg ggatatcaga agaaatctca gaaaaaatat
    15481 ttacgctagg tttttcgacg aagggaatca atcgtgggtt tggattgaat ctcatccatt
    15541 caatcgtcaa acgccacggt ggatttttag aattttccaa acaaacaaat ggcggtacaa
    15601 tcgtctatct agaattacca aaaaaggaag atcaaaatga tgacaaatgt cctgataatc
    15661 gaagatgatc caatggttgc agaattgaat cgtacttttg tggaaagcat ccctaatatg
    15721 aacgtaatcg aaagtttcag aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac
    15781 gcagatctgc tgctgcttga tgtatacatg cctggcgaaa gtggtatcca ttttttatct
    15841 tggcttagac agcaaggaaa tgatatccca gtgattttga tcactgctgc agatgatatc
    15901 gaaaccataa agaatgtgat ccgctacggt gccttagatt atttgatcaa accgttcact
    15961 ttgcaacgtt ttcagctggc gatcgaaaag tatcttgctt tctctcagac aatccaaacg
    16021 actgaaaaag ccgatcagaa attgatcgat caagtattca accacaccac tcccattcac
    16081 gaaataccat cattcatcga tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata
    16141 agtgcattta ttctaaaaga agatactatt ttttcaacaa aagaactggc tcaaaaaagc
    16201 tcactgtcac gtatatccgt caagaaatat ttgaattttt tagtagaaca agatatattg
    16261 aacgaaagaa tcaaatattt ggaaatcggc cagccgctaa cctgttatga attgacaaag
    16321 aatgggcgga aaaagctcac agattactgt tgagtcatcg aattatccca tacaaaatag
    16381 tccaagtata cagaagtaac aactgtttac ttggactatt tttaaacatt ttattgacga
    16441 atatccatgt tttcttatct taattgacgg gctttaggaa gagatacctc atttttcgtt
    16501 tttccagttt ctattatttc acgaaagttc tcgaaacttg tcgaaatcat atttgataat
    16561 gcctcatctg tatttgaacc tacatgcggg gttacaagaa ctctagggta catttctact
    16621 aattgctgga tagcagggtc tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc
    16681 tctgtttcat ttgcaaaaac atcggttgca aaaccagcta aatgattgct tttcaaagct
    16741 cttaaaatag cttcattatc ttgaagctct ccacgggctg tattaatcaa gatgctacct
    16801 tttttcattt gagctaaaaa tccttcattg atcatttgat cgttttcacc tgggatatga
    16861 ggtacatgaa tactgacaat atcgcttttg gctaataact cttcaagcga gtggaacgtc
    16921 aatacttttt tagcagcttc tgtctgatga atatcgtagc cgatcacttt ggctcctagt
    16981 cccttaaaca aaacagcttc cgtcaaccct atcctgcctg ttcctatgat ccctactgtg
    17041 caatttctga tttcacgact aaacatttgc tcatccactc gaaaatcttt gaaagatgtt
    17101 cggatcgtcg tatacgcagt atttctcaaa agcatcatgg caaaagtcaa agataactct
    17161 gctatcgcat ttggcgaata cgatggtaca cgggctactg tcataccaaa atcagctgcc
    17221 gcttgtagat cgatatgatt gactcccaca gtccgtgtaa aaacataacg aatcccatat
    17281 tcctgcattt ttgcaagatt ggtctcatct gctatacaat ttcccctcaa caatacagca
    17341 tcatggtcct ttgctgacac gatattgtca tgtgtcaaca gttcttcata aagactgaga
    17401 tcataatggt aaatattcaa tttttcaaaa aaatctatct cgttcggacg aacaccgtaa
    17461 catgcgattc tcatactttc ttcctccatt tcttatgaaa ataaacctat gccatgaatc
    17521 atttgtaata caacgatcca tacaggtgtc agtaccacag caaaaatagt tgacagtaag
    17581 gatgcattgg aggctaaaag tgcctcccta tcaaaactga tagcataggc tgctgccact
    17641 gttgcagctg gtgtagccat catgataacg attgttgcca gtgcttcaaa attgactggt
    17701 agaatattcg ttgccgtcaa aactgctaga atcacgatat tcaatgcagg tacaaatacc
    17761 actttgacta gactgtaata ccatgaagtt ttttcagatg cagctttttt gaagcttact
    17821 tcacctaatg ttgcgccgat tgctagccac gccaatggtg aacttaaacc agctaaataa
    17881 tccatcggct gaaatagcca cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt
    17941 atctctcctt ctgcacctgt aactgacact tgtggcagat acccttggaa cagccaaatg
    18001 aaaaggccgg caaaagtagc gataatgatt ggattgacga acatcgcttt gatattttta
    18061 gcttccatct tcaagccact catttttatg tatccataag aatacaaaaa gattcggtag
    18121 ccaatattga aaattgagga atacatcacg ccgacaggac cataaatcgc actgacgata
    18181 ggcgttccga aaaaggtcgt agaaccaaag atcgtcagga cacgcaacgt tgtttgctta
    18241 tcccctttat agcttaagaa aaaaggtttt gaaactagaa taagaataat ataaataact
    18301 aatccccaaa tcaagacatt gattccttgc atcagtaatt ctgtattgat atcttgcatg
    18361 aaagctcgaa atgccagtgc tggcaaagcc actgttaaga cgactttcga tagaattttt
    18421 ccaacagatt cagaaaaaat cccctttttt ctacaaacaa accctaataa tataataaaa
    18481 actgttgaag tgattgcact gacaatcgac atatctgtca aagtcgatcg aaaaatgtca
    18541 aataagttca tctttctcct cctaattatg ttcatttatt cacaataaaa aaagctttta
    18601 ttttgctaat acgtattaac atttttaatt ttatctttac attgttactt attgaacaat
    18661 aggaaattaa agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa
    18721 ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg
    18781 aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata
    18841 aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt
    18901 gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca
    18961 acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga
    19021 cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc
    19081 atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc
    19141 attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag
    19201 acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc
    19261 ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct
    19321 tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg
    19381 acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct
    19441 gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt
    19501 tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta
    19561 gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct
    19621 ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca
    19681 gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga
    19741 atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact
    19801 tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag
    19861 cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt
    19921 ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga
    19981 atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca
    20041 tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat
    20101 aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc
    20161 tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa
    20221 atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt
    20281 cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata
    20341 caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag
    20401 cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg
    20461 caaacaccga gataataata atagcgttga aagtcagttg cttgatacag attttcagaa
    20521 atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta
    20581 tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca
    20641 tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt
    20701 tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt
    20761 ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt
    20821 aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact
    20881 ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta
    20941 agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa
    21001 tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct
    21061 gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc
    21121 caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg
    21181 aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa
    21241 gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat
    21301 tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg
    21361 cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac
    21421 aacatattca gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc
    21481 cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg
    21541 aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga
    21601 tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct
    21661 tctaataatt cttttggata aagtgcccca gtaggattat taggattaat gataacaata
    21721 gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc
    21781 tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac
    21841 aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata
    21901 cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc
    21961 ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg
    22021 gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt
    22081 ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc
    22141 tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct
    22201 tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag
    22261 ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa
    22321 ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac
    22381 ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat
    22441 gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa
    22501 gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat
    22561 ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat
    22621 gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt
    22681 cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt
    22741 ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac
    22801 aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac
    22861 ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc
    22921 gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc
    22981 acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct
    23041 ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt
    23101 ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc
    23161 atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca
    23221 attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa
    23281 agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc
    23341 tttgccctt
//
LOCUS       DACDBG010000040        22866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDBG010000040 DACDBG010000000
VERSION     DACDBG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.040"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(226..447)
                     /locus_tag="IVZ90_001556"
     CDS             complement(226..447)
                     /locus_tag="IVZ90_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262610.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(609..681)
                     /locus_tag="IVZ90_001557"
     tRNA            complement(609..681)
                     /locus_tag="IVZ90_001557"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(646..648),aa:Lys,seq:ctt)
     gene            757..987
                     /locus_tag="IVZ90_001558"
     CDS             757..987
                     /locus_tag="IVZ90_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8262611.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1031..2200
                     /locus_tag="IVZ90_001559"
     CDS             1031..2200
                     /locus_tag="IVZ90_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8262612.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            2774..3343
                     /locus_tag="IVZ90_001560"
     CDS             2774..3343
                     /locus_tag="IVZ90_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8262613.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3330..4190
                     /locus_tag="IVZ90_001561"
     CDS             3330..4190
                     /locus_tag="IVZ90_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8262614.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4380..5084
                     /locus_tag="IVZ90_001562"
     CDS             4380..5084
                     /locus_tag="IVZ90_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262615.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5089..6924
                     /gene="walK"
                     /locus_tag="IVZ90_001563"
     CDS             5089..6924
                     /gene="walK"
                     /locus_tag="IVZ90_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8262616.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6921..8237
                     /locus_tag="IVZ90_001564"
     CDS             6921..8237
                     /locus_tag="IVZ90_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262617.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8238..9107
                     /locus_tag="IVZ90_001565"
     CDS             8238..9107
                     /locus_tag="IVZ90_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262618.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9162..9971
                     /locus_tag="IVZ90_001566"
     CDS             9162..9971
                     /locus_tag="IVZ90_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8262619.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10074..10703)
                     /locus_tag="IVZ90_001567"
     CDS             complement(10074..10703)
                     /locus_tag="IVZ90_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8262620.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10873..12165
                     /locus_tag="IVZ90_001568"
     CDS             10873..12165
                     /locus_tag="IVZ90_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8262621.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12241..12903)
                     /locus_tag="IVZ90_001569"
     CDS             complement(12241..12903)
                     /locus_tag="IVZ90_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8262622.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13128..13412
                     /locus_tag="IVZ90_001570"
     CDS             13128..13412
                     /locus_tag="IVZ90_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8262623.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13463..15088
                     /gene="groL"
                     /locus_tag="IVZ90_001571"
     CDS             13463..15088
                     /gene="groL"
                     /locus_tag="IVZ90_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8262624.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(15177..16325)
                     /locus_tag="IVZ90_001572"
     CDS             complement(15177..16325)
                     /locus_tag="IVZ90_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8262625.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(16427..17011)
                     /locus_tag="IVZ90_001573"
     CDS             complement(16427..17011)
                     /locus_tag="IVZ90_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8262626.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(17138..17569)
                     /locus_tag="IVZ90_001574"
     CDS             complement(17138..17569)
                     /locus_tag="IVZ90_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8262627.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(17587..17907)
                     /locus_tag="IVZ90_001575"
     CDS             complement(17587..17907)
                     /locus_tag="IVZ90_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262628.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            18645..18902
                     /locus_tag="IVZ90_001576"
     CDS             18645..18902
                     /locus_tag="IVZ90_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8262629.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(18873..19181)
                     /locus_tag="IVZ90_001577"
     CDS             complement(18873..19181)
                     /locus_tag="IVZ90_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262630.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            19259..20005
                     /locus_tag="IVZ90_001578"
     CDS             19259..20005
                     /locus_tag="IVZ90_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8262631.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            20020..20223
                     /locus_tag="IVZ90_001579"
     CDS             20020..20223
                     /locus_tag="IVZ90_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8262632.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            20328..20627
                     /locus_tag="IVZ90_001580"
     CDS             20328..20627
                     /locus_tag="IVZ90_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262633.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            20627..20809
                     /locus_tag="IVZ90_001581"
     CDS             20627..20809
                     /locus_tag="IVZ90_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262634.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(20815..21291)
                     /locus_tag="IVZ90_001582"
     CDS             complement(20815..21291)
                     /locus_tag="IVZ90_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262635.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            21344..22069
                     /locus_tag="IVZ90_001583"
     CDS             21344..22069
                     /locus_tag="IVZ90_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8262636.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            22243..22584
                     /locus_tag="IVZ90_001584"
     CDS             22243..22584
                     /locus_tag="IVZ90_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262637.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            22577..>22866
                     /locus_tag="IVZ90_001585"
     CDS             22577..>22866
                     /locus_tag="IVZ90_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8262638.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7801 a         3773 c         4538 g         6754 t
ORIGIN      
        1 atttaaatac attctatcag tacgttaaca cttcaaggag catattttag ttgcgccatg
       61 atttcttact gtaaagaagg agcgataaac attctaaaat aatttttata gtcaataaaa
      121 agcgatgatt tgaagttggg ataatgatac ttccgtaagg ctcgccgtaa acttggagag
      181 aggtgaaatt cctatgtgct tttgacaaaa gcagctgatt aagaattatt tccatatgtt
      241 gatttcaaca ttttttctat tatttttaag accaaattca gtgaggcaaa tagcatcatg
      301 taaatttagt ttttctattg aacgttctcc actttttagt ttttttatag tactcaaatt
      361 taatcctgta tgtttagaga tctcatttgc agtatggttt tgtaaagttt ctcggattat
      421 tgttatatta gcaatttcat tacgcataat tatgttcctt tcttatcaaa aatatcaatg
      481 actacagtgg gcttttagtg aaaaaaaggg gtactacagt ctcttttgaa aacaaaaaaa
      541 tacagtatat acgttttcta aatgctaagc accaacgttt acactgtatt taagggtttg
      601 gcacccaatg ggctgtgagg ggctcgaacc cgcgacccga tgattaagag tcatctgctc
      661 taccaactga gctaacagcc caaattaata ctttgttatt ctaacaccta aaataaaaaa
      721 gtgcaatact tattatcaga attgagagtg aattttatgg attttcaaga ttatgaaaaa
      781 atatttgaaa gttatccatc gatattgaaa attactgatt taactaaaat gttaaatatt
      841 ggaaaaaata aagcttataa tttgctgaaa gatagatcaa tagaaaatat aaaaattgga
      901 gcaaggtaca aaatacctaa aaaagctgtc attgagtata tattgaaaat acaagatgaa
      961 ggtaaaaatc atgctataat aaaatagtca aaatttttgc cgatatttca gtttaagagg
     1021 agagattgaa ttgaaattaa gagcaaacat aattgaaaaa agaggaacat tacaagtagt
     1081 tgtatcttat aaagactcta acggaaaaat tagacaaaag tggaaagata caaaattaaa
     1141 aaagaaagga aataaaaaac gagcagaaaa aattagagat gaatttttac tagaattgga
     1201 aagtcaatta gctatggaat gtgaatgtgc taatccaaat ttattgtttt atgactattt
     1261 gcttaattat ttggaagtat caaaaaaaca agtagcattt aacacatatg ttgggtataa
     1321 gcattacgtc cataatcgaa tttacaagtt tttttatcct aaaaaaatta aactaaatag
     1381 gttaaagcct tatcatttac aagagtttta tcaatatatg ttaaatgata actgtacagc
     1441 aaatacggtt attcattatc acgcatttat acgcaaagct cttcaagagg ctgttattac
     1501 agaattgata agtacaaatg tagcagataa agtaagaaag ccaaaaaaga aacaatttgt
     1561 ttctcaagtt tataatcaac aagagatctt gaaattactt gacattttgt cagaagaaaa
     1621 actctatttg gtagttcttt taacagcatt ttatggattg cgaagaagtg aagttttagg
     1681 attaaaatgg tctgctattg attttttaaa caaaaaaatc atgataaacc atgtaatcat
     1741 agaaaatcca gagaatatgt cacagttaat aaagaaagat caaacaaaga actcttccag
     1801 ctatcgtaca ttacctttgg ttgaaacgat tgaacgagca ttagtaaatc aagctaaatg
     1861 gcaacaagat aatcgtattc ttttaggaga tgattatatt ttagaagata atgagtacgt
     1921 atttactatg gaagatggac ggttgatgaa gcctcaatat gtaacgcatc gtttgagtaa
     1981 gttgattaag aaaaatggat tgaaaaaaat tcgctttcat gacttgcgtc attctaatgc
     2041 ttcaataatg ttagatagtg gccagaatat gaaatccatt caagaatggc tgggacatgc
     2101 atcgtattcg acaactgcta atttatacac gcatttaacc gcaggagtaa aagatcgtgc
     2161 agcagaagca ttagaaaacg catttgggtt taaagaataa aaaaacaaaa aacaatatct
     2221 ttggggtatc gcttcttgtt tttagaattt ttttgttggc aatttgttgg cagtatgttg
     2281 gcaatgaaaa aattcacaaa ctaaaaacct atttataaag ctttataaaa cttttttcac
     2341 aaatagagca tttggttaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa
     2401 aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat
     2461 cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta
     2521 ttatataagc ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc
     2581 attttttact cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac
     2641 aagaaggctt agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag
     2701 ttaaagcaac tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa
     2761 aggagcggtt atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga
     2821 aacattccgt ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt
     2881 cagaacgtta gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct
     2941 tacctcttct atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa
     3001 aagatataac aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt
     3061 gactattcct gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga
     3121 agcctataat ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc
     3181 acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt
     3241 aagagcggca actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga
     3301 aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag
     3361 attcaggtac ggatgttcca gaagaattga taaaaaaata cgagatgtat atgatcccat
     3421 tgaaaattat ttataaagat caggtatata cagataaaat tgacattacc cctgaagaga
     3481 tctatcaacg tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca
     3541 cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt
     3601 ccagtggatt gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat
     3661 tggaagcata tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc
     3721 aagcagcaaa atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg
     3781 agttagtcga aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag
     3841 gtggacggat cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca
     3901 tctcatgtaa tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa
     3961 gtttggataa aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact
     4021 tagctgttgc ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa
     4081 gagagttccc acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc
     4141 acactggtcc gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa
     4201 ggaaacactg aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa
     4261 taaagtattt tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca
     4321 aaagcagaaa tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta
     4381 tgaaaaaagt tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt
     4441 tagccaaaga aggctatgac gtttatactg catatgatgg ggaagaagcc ttggaaaaag
     4501 ttgctgaggt agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct
     4561 tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca
     4621 aagattctga aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca
     4681 agccattttc caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca
     4741 cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga
     4801 tccatccaga agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg
     4861 aattcgaact actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt
     4921 tattacaaac ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg
     4981 tacgtcgttt gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac
     5041 gtcgtggtgt cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa
     5101 gtccgctttt ttcgatcagt caattttaag atcgctatta cctttattct gattttattg
     5161 atctcgatcg aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat
     5221 acatttatta aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa
     5281 ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt
     5341 gaaaacagtg cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc
     5401 cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat
     5461 atcaatgatt tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat
     5521 atcaatgttc aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc
     5581 aaaagtaata tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact
     5641 gcctcactaa ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt
     5701 acacagccga tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt
     5761 cggaaagtca aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag
     5821 ttagctgaac gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat
     5881 agcgtgctat cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt
     5941 acgatcaatg atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa
     6001 tcgattttga cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat
     6061 ccaaacgaaa tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg
     6121 cgagtagact tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc
     6181 ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat
     6241 gtttcccatg aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc
     6301 gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa
     6361 acagaccgaa tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt
     6421 aatacccagc ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac
     6481 cggtttgata tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg
     6541 caaagagatc tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc
     6601 atgaataacg cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa
     6661 acgcataata acgtgatttt aagtatcacc gatcaaggat tggggattcc taaaaaagat
     6721 ctgaaccgag tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt
     6781 gggacaggac ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt
     6841 tgggcagaga gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct
     6901 tatgaggagg aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt
     6961 tgatggttgc gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa
     7021 gttcaatcaa cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa
     7081 cttccgaagt ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa
     7141 ccaacagtga aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa
     7201 agctttctga agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact
     7261 ctggttttga gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg
     7321 atttagattt agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa
     7381 tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat
     7441 atgaagcaaa tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca
     7501 caaaatggac agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac
     7561 attcgatcaa actgaagaag tatagttata tcctgtcaca gcaaccctat acacttttta
     7621 gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga
     7681 ttttttacgg tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc
     7741 gtggagagct gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata
     7801 ttagcaaatt aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga
     7861 tcgattatcg gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga
     7921 ttggtgtgga agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt
     7981 tgaatacgat tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg
     8041 aaatcatcca gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg
     8101 ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat
     8161 ggtatgtgaa atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg
     8221 gattggaggc gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt
     8281 tagggttgaa tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg
     8341 tcattcgctc taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt
     8401 acacggggaa gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca
     8461 atcttagcac ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg
     8521 gtgtgttttt agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga
     8581 acgtgatcga tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca
     8641 atattttatt cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac
     8701 cactagaaat ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa
     8761 aaatcgttct ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga
     8821 cgcatttatc tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag
     8881 caatcaatac attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc
     8941 agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc
     9001 atgtagcgat cgaaacaagc gataaagtgg tccaaattga gagcgtcaac gctttcagca
     9061 atacgatcgt gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata
     9121 atgaaaaaag tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag
     9181 cttttaaaat cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg
     9241 aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg
     9301 ctcaaattga tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag
     9361 atcatataca tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc
     9421 agcagacttg ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc
     9481 tatttgagat ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct
     9541 cccatgacgc agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca
     9601 tgttgacgga caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg
     9661 gttatttgat ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga
     9721 atttaaaaca gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg
     9781 tcatgactga tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg
     9841 aaaacaatat gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt
     9901 ttggcgttgg acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat
     9961 tttcgattta aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg
    10021 gttggtttta tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga
    10081 ctgactacac tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca
    10141 atcgcgtaat ctgccatgct gatcgtactg tcgctagcag cgtttctttg taaataatca
    10201 tcacttaaca aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg
    10261 atataagtcc aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg
    10321 aaagctgttg gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt
    10381 tctgtatcta agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct
    10441 tcaagcatct caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga
    10501 gcgttgaaag catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat
    10561 aaatcctttt gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc
    10621 gtcacgtcta aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta
    10681 gctccaatga gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca
    10741 gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat
    10801 ggttcgtgat cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga
    10861 aggagataag aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt
    10921 gtgaatctta gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt
    10981 attgttggct cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg
    11041 atagacaaaa caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg
    11101 atagcctttt ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat
    11161 cgcttaccag tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat
    11221 actcagatag atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg
    11281 ctcatcatcg gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga
    11341 gagaagatac ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata
    11401 gtcactttta cgcttatcgg ttcagcggtt tttcaacatt tgttcagaat gactgttgtt
    11461 caaggagtat ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt
    11521 gtgcttttat taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg
    11581 atggtcaatg ggttggttag tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc
    11641 ttagcctcag gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt
    11701 ctcgatcagt tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg
    11761 acggtttttg cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc
    11821 tttttcaaca tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg
    11881 gctgttcctt ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat
    11941 tttggattcg ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc
    12001 tcgggtttct tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt
    12061 agttttttca tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa
    12121 agacagacaa aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc
    12181 ctgtcgcttt agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt
    12241 ttatactagc cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac
    12301 gtgtgtgatc atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa
    12361 acctaggaaa aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga
    12421 gctgatcact gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg
    12481 gcggaatacg aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg
    12541 tgcttccatg atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa
    12601 caatacagat tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac
    12661 caatccataa ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa
    12721 gggttcacgt tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac
    12781 tgcagttgct gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa
    12841 gggtgagata aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga
    12901 catgtaattc ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt
    12961 tcaattatat cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta
    13021 aaagcttgca aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg
    13081 ttaagagtgc taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat
    13141 taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg
    13201 ttttagcttc tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag
    13261 gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta
    13321 tgttcgaaaa atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg
    13381 caggaaaaga tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa
    13441 taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc
    13501 agctatgtta cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa
    13561 aggccgaaat gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt
    13621 gacgatcgca aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt
    13681 atcagaagta gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt
    13741 tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct
    13801 aggcattcgt cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat
    13861 ctcaactgtt gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc
    13921 tgataaagtt ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat
    13981 cacaatcgaa gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt
    14041 tgatcgtggt tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct
    14101 agaaaaccca tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc
    14161 attgttagaa caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga
    14221 tggtgaagca ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc
    14281 tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt
    14341 gacaggtggt acagtaatta ctgacgatct aggtttagaa ttgaaagatg caacaatcga
    14401 aaacttagga aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg
    14461 ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga
    14521 aacaacttct gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg
    14581 tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg
    14641 cattgaagat gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg
    14701 tggtacagct ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt
    14761 ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga
    14821 aaacgctggt tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac
    14881 tggtttcaat gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc
    14941 aactaaagta acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac
    15001 gacagaagca gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga
    15061 tccatcaatg atgggcggta tgatgtagta attaagaaag aaccctattt taacagggtt
    15121 ctttcttctt tttttgtttt attttaagca aaaggggcaa agaaggggca aaaatatcaa
    15181 aggctattta gtttatcaat tacttgttgt ttggctcttt ttgttacatg gttataaata
    15241 gacagagttg tgtttgcatc agaatgccct acacgctcca taatggcttt aagaggtacg
    15301 cctaattcag ataatagtga aacgtggcta tgtctgaata tatgtgagga tagacttttt
    15361 tctaattcca actcttcctc tactttatgg agtatagcgt taaatgagtg cagcgcaagc
    15421 ggagtgccac ttgtagatat aaatatatat tgatcgggat ctgtgggtct acctgcaaga
    15481 atattgtcag ctatcacact ttcaatcaat tcttttgcac gattgggtaa ttgcacttca
    15541 cgttgcgaat aagtattttt tggagttgtt tttatagcat tatccatttt cacagatgtg
    15601 taatctaagg tcccattaat ggaaattttc ccatcctcat agtccttcat ttgcaaagct
    15661 agcaattccc catatctcaa accagttaaa tatagaaact cagctattat gccgtgtagt
    15721 tttctgcgag gattggagta tagctgtttc agtatttgat caatttcttc tttctccaga
    15781 tattttttat tcatagaaag ccttcttttt tcttcttcca cttttttagg atggatttta
    15841 actgctagcg cagggtttct ttgaatgtat tttctatcga ttgcatagtt tagcataaca
    15901 gatagagttg tttttgtttg ttttgtgtag ttcagtgaga ggtcaccaaa cgtatacata
    15961 tcttcaatta tcttattaat gagtgtctca tcaatgtttc taacgatcgt atcatcgctt
    16021 atgtgcttag aaacatgttt catcatcatt ggaaccttca aatagctagt acgtttaaca
    16081 tgctgcttat aatattcata ccattcttta tacagctcac caaaagtgat atctgattta
    16141 ttgtaatctt cgagtgcttc tttaattttt ttatctagaa ttttctgagc ttttttccac
    16201 gcttgtggtg aattacttgt aagtgttgta gatttttttc gtgttttttc tgtataagga
    16261 tctacatatc tttcaataaa cttgaattgg ccatctttgg tctgttcaac ccacactttt
    16321 aacatctcct atcatttgct ataataggca taacaaatag acctatatag gtttgttttc
    16381 taaaagcacg ctcttactct ggacggtggg gcgtgttttt tatttattat acagttgctt
    16441 taaaaatagc tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct
    16501 tttggtttaa cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca
    16561 ttgtattttc gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat
    16621 ctgtacctac agggatatct gaatcgccat cattttttac ggtataagta atttttacta
    16681 cttgtgcagg ttggttttct tcaaattgat ttctttcatc agttagttct acactattta
    16741 gtgtatattc agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt
    16801 tttcttcttt agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat
    16861 cacttgcttt attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa
    16921 ttactgctaa tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt
    16981 ttccgtcttg acccataact ttttttgcca tttaaatatt cctcacttct tgttataata
    17041 tatttgcgat ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt
    17101 ttgctgaaat cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag
    17161 aatatttttt cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct
    17221 gaatagaata atactccata aatttttcga gattaaactg agattcatca gtcagttcgt
    17281 tctcaatata gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt
    17341 ttgagtgaaa agttgtagaa gtatcgtaca aaactgaata ttcaaaatga gaagcggtga
    17401 aatgcgcaag ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat
    17461 tcaaaaaaat gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa
    17521 ctactttcaa attgtattcg ctcaacattt ctttcaattt caaatacata caacccatca
    17581 ctccaactat tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc
    17641 cttagatatt tcttttccat caaaagaaaa aacagtatcg ttttctgata aatccacatg
    17701 tttaggggtt tctctttctt ctctacctag aaggtagtct acagagactc caaagtaatc
    17761 agctaaccga gctaactcaa tagaattagg agtttgtctt ttccaacttc ctaaatatcc
    17821 attcgaatac ccgaaattca tttcgagctg tctaatggat aatccttttt cttttgctaa
    17881 ctcctttatt atttcgtaag tattcattga taaatcaacc tttctgaatg cttacaaaaa
    17941 aagtttagat aaataatgta aaaatagttg acttatatac attattaatc tatactatgt
    18001 tttgtaaaca agtttaacaa ctaaaaagac aacaaaaaat actattgatt aataaatgcc
    18061 aaccgccaag aaagcttttt aaatcaaagt ttatatgtct tatttaacta tgcatttagt
    18121 atagattatt aatctaataa agtcaacgca gttttttaaa aagttgttaa atttgtttac
    18181 gaatataaaa gaaaggagag aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt
    18241 tgaaattaga catactgaaa caagcaaaag tggcgatgga acacgcggta gataaagaag
    18301 actctgccat ggttgcagcc atagcagaga ttttagctca cgtttagtca tcgtttttac
    18361 ggtcttctgg aagctgacca tagataacag cataatgaat atctaaataa gcttgaacaa
    18421 tttcttttgg cgaaattgat tcgcctttag tagatacttg tgattcgtga tttagccatg
    18481 caacaactaa atccgcagcg atttcaaacg gcgttacact ttgtgtcagt tgccataaag
    18541 aatttcacaa tatgtatggt cgtggtaata ataccaaaga gcaatttgag gaatttatgg
    18601 aaattaaaaa atacaaaaaa tcatttatta aaggaggcta aacaatgaac aaggaaatga
    18661 gccaaacaat caaggtacaa aaaatgatag atgatctaac acatggaatt gatagccaag
    18721 ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca
    18781 agactatcaa ctttgaaatg aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa
    18841 agttggcttc cgctatcaac gagcaagaat tactatttga aaccgatact tttaacagct
    18901 gatgttttat agatgacagt ttcatccgaa tctatatcat cgccgagaga aaagaatgtt
    18961 gcttttgaca aaaagccgat aagacctaca agcggtcttg atgttgcaag agtagatacc
    19021 ccaatatcac cttgcaaggt attcacgtaa atttgagaag aatagaacat tccatctttt
    19081 tcttcgatat ttgacgcatc atcgtatcca acgaccaaag tatcggcaaa tattttgatt
    19141 ttttctccat caagcatagt aattacaaat tctggtttca taaattttca cctccttaac
    19201 aattatttca gcctgtcaca ctgataagga aattatacca aagaaaggaa tgagaaatat
    19261 gaatacacca caaattttta atttcgagca aaacgaagtt cgaactattt tagtaaatga
    19321 tgaaccatat tttgtaggaa aagatgtagc agacgttcta ggatattcga atccgcaaaa
    19381 agctattcga gatcatgtgg acttggagga taagacgcag aacgattcgt tcaccgtcaa
    19441 tggaacagca gttgttttga ttaacgagtc aggcctttac agtttaatct taaaatcaaa
    19501 acttccctct gccaaaaaat ttaaacgttg ggtaacgagt gaagtccttc caacaattag
    19561 aaaaacaggt agctattcaa acgtacctca aagttttgca caagcattgc gtttagcagc
    19621 ggatttagaa gaaaagaacc aattactcga acaacaaatt gccgagtacg aaccaaagat
    19681 tagctactta gatacgattc tttcatcgac agatacggta gctacttctc aaattgcagc
    19741 tgattacgga atgtcggcaa ttgctctaaa caaattgcta aacgagttag gtgttcagca
    19801 taaagttagc ggacaatgga tactttaccg aaaacatatg aaccaaggat acacaaaatc
    19861 gcacacaagt gagataccga aagccgatgg cggcactaaa gttgtaatga ataccaaatg
    19921 gacacagaaa gggcgagtgt ttatttacaa cttattaatc gcagagggct attaccctca
    19981 aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc
    20041 actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac
    20101 accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt
    20161 aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg
    20221 tagagggagg taaagtcgtt ggaagataaa atcattctta caaaatcaga gcttcaagaa
    20281 atgttaaaca tcgagtatgg aacgataatt tttaggaggg ataataagtg aaaaaaccaa
    20341 cactttcaga gctgatagaa gctgctgaga aggcagtaaa accagacgac tggtaccgac
    20401 aaagtttaat cttggagaag ttccacggca tgtcaaaaac tactttagtt gaatactgca
    20461 aggaaatgga aacaattcct gaatttgcag aaggaatact tcgtccaggt cactcaacca
    20521 catttattca ttaccatact tttatttggt ttttaaagtg gaaagacgca aacaaatatc
    20581 gtgtaaaaag tttatcgcct tcagatgttt tgaaggaggc aagttaatgg gcaaattcaa
    20641 cagagcacta gtattcagcg caccgctaat catctacgct ttaggcctct ggggaagcag
    20701 acaagcgttg atagggacaa tcgtttacat ggtttggatt tttatggggc ttgatgaagc
    20761 tgagcacaga gcgaaaaaag ccagtcggga gggactgact aaaatatgat ttttttattt
    20821 ttgttccttt gggctgctat tgcgtacttc atccagctcc ttgttgtcaa agttgctctt
    20881 atttctttta ggaagaacaa aagaaaatag cgctataaat gaaagataag tagccgctac
    20941 attaaactgt tcttttgaaa aagttacagt catcggaatg ataattgata ttgaggaagt
    21001 aatcttaatt aaaaatatag caatattttc aacatcgcgt tggtttttat ttaacattgt
    21061 ccaacatgtt ttagcaacaa taaaaataat tacacatgat attagcacac agactgaaaa
    21121 aagttttaga aatgcgatgt agtcagtgtt attgaactcc ttcaaaatac tatttttatt
    21181 caatgtaata aaaggaattt ttttaattga agacaggaaa gataaggtaa taaacagaat
    21241 tatatatatg gttaaagtcg cagtaagaat gtaattgagc tttttcctca tagaatcacc
    21301 tcactttcta acgaaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
    21361 tttgaaaaat aaagaagctg taactacgag cttacaagtg gcagaaagtt tcaaaaagaa
    21421 acataagcat gtactagaag caattgaatc aataaaaaga tcggtcgaaa attcggccaa
    21481 tgttgaagat gggtccaatt ttggacagat gtttgtggaa gggaacgagc cagactcata
    21541 cggaagaagt cggagagttt atttcatgaa tagagacgga ttttccttgc tagctatggg
    21601 attcactgga agtaaagcaa taaatttcaa actaaaattt attgaagctt tcaacgaaat
    21661 ggaagatgtt attcggaaga atactgttcc tcaaacaatt gaagacatga tgatctatca
    21721 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
    21781 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tggaagttct
    21841 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
    21901 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
    21961 cgatgatgct gtttcattta ttgaaatgtg gttaccagaa actgcgatcc gcatggaaat
    22021 cgatcaactg aacagacaac aaagactttt cggtgatgaa aatgaataga gctgaagcgc
    22081 taagaatagg gacggtaatt gctaatcgct ggtggagaca caataaacca aacatcctaa
    22141 gccaacaaca tatcgaaaag caaaaagcat ggcaacaaat aaaaaagtga ctcagccggc
    22201 aagcatagag tcacaaaaca tatctaagga gaattttagc atatgaataa agaactttcc
    22261 actttagatc aatatttgac tgattctgaa tggggcaagt cgaatatcaa ggaaacaaat
    22321 aatcgaaaaa tcagacgaaa tcttttgacg aacgaagaac tatcatgtga tcaagatgac
    22381 ttgggaaatt ttgtgagtat ttgggatcat gtttacctta tccatctatc aaaacattca
    22441 aataaacctg aatacatcta tgtcatcgaa gatggcttga ttgatgcgct agaagagtac
    22501 aacagagata acttgattga tatctcttat tacggatcag gtaagaaata cattgctgaa
    22561 atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag
    22621 tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca
    22681 cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtataa
    22741 acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt
    22801 tcttatggat gaaacaggga cttatgtatc tgtctcagta acggttaaag gacatacgga
    22861 aacaga
//
LOCUS       DACDBG010000041        21804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDBG010000041 DACDBG010000000
VERSION     DACDBG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.041"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..637
                     /locus_tag="IVZ90_001586"
     CDS             83..637
                     /locus_tag="IVZ90_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8262639.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(810..1283)
                     /locus_tag="IVZ90_001587"
     CDS             complement(810..1283)
                     /locus_tag="IVZ90_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8262640.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1295..1831
                     /locus_tag="IVZ90_001588"
     CDS             1295..1831
                     /locus_tag="IVZ90_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8262641.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1871..2242
                     /locus_tag="IVZ90_001589"
     CDS             1871..2242
                     /locus_tag="IVZ90_001589"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8262642.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2526..3797
                     /locus_tag="IVZ90_001590"
     CDS             2526..3797
                     /locus_tag="IVZ90_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8262643.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4042..5067
                     /locus_tag="IVZ90_001591"
     CDS             4042..5067
                     /locus_tag="IVZ90_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8262644.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5361..6470
                     /locus_tag="IVZ90_001592"
     CDS             5361..6470
                     /locus_tag="IVZ90_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8262645.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6553..7449
                     /locus_tag="IVZ90_001593"
                     /pseudo
     CDS             6553..7449
                     /locus_tag="IVZ90_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7465..8289
                     /locus_tag="IVZ90_001594"
     CDS             7465..8289
                     /locus_tag="IVZ90_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262646.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8321..9631
                     /locus_tag="IVZ90_001595"
     CDS             8321..9631
                     /locus_tag="IVZ90_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8262647.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9779..11038
                     /locus_tag="IVZ90_001596"
     CDS             9779..11038
                     /locus_tag="IVZ90_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8262648.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11048..11920
                     /locus_tag="IVZ90_001597"
     CDS             11048..11920
                     /locus_tag="IVZ90_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8262649.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11932..12762
                     /locus_tag="IVZ90_001598"
     CDS             11932..12762
                     /locus_tag="IVZ90_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262650.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12802..14985
                     /locus_tag="IVZ90_001599"
     CDS             12802..14985
                     /locus_tag="IVZ90_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8262651.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15046..16266
                     /locus_tag="IVZ90_001600"
     CDS             15046..16266
                     /locus_tag="IVZ90_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8262652.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16263..17723
                     /gene="gtfA"
                     /locus_tag="IVZ90_001601"
     CDS             16263..17723
                     /gene="gtfA"
                     /locus_tag="IVZ90_001601"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8262653.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17800..18906
                     /locus_tag="IVZ90_001602"
                     /pseudo
     CDS             17800..18906
                     /locus_tag="IVZ90_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19228..19437
                     /locus_tag="IVZ90_001603"
     CDS             19228..19437
                     /locus_tag="IVZ90_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8262654.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19430..19579
                     /locus_tag="IVZ90_001604"
     CDS             19430..19579
                     /locus_tag="IVZ90_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8262655.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19614..20398
                     /locus_tag="IVZ90_001605"
                     /pseudo
     CDS             19614..20398
                     /locus_tag="IVZ90_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20682..21767)
                     /gene="xerS"
                     /locus_tag="IVZ90_001606"
     CDS             complement(20682..21767)
                     /gene="xerS"
                     /locus_tag="IVZ90_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8262656.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7409 a         3285 c         4202 g         6908 t
ORIGIN      
        1 agtttatata tataagttaa tgattatggt aaaatatata cataatcatt aactcagaat
       61 atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga caaatgaaga
      121 gatacaagat tttcagtttt ggctaagaag aacaagtaat ccggaaagag atagtttttt
      181 atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat taaaagttgg
      241 agatatacga ggcaaaagta agcctattat tgttgaacat aaaacaggta agcgaaaacc
      301 gatttttata gataatttga gagaagaaat tttattatat actgaaggta aagaagaaaa
      361 tgattggtta tttccgagtc ggcaacaagg tcgacatata acaagggatc gtgtctatca
      421 gatatatgca gatatagcag aaaaattagg acgagatgat ataggaacac atacattaag
      481 aaaaactttt gggtatcact actacaagaa aactagagac attgctacct taatgtttat
      541 ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg aagatgaaat
      601 aggagcatca cttcgtggat ttaaactagg tgtataattt aaaaattttt ataaacagta
      661 aagacaaaga ttaaaagcta tttttatcta gaacgatttt atttcataaa gttcgcggaa
      721 taaaagctgg ggttactggt acttggtata ggttcgtgtt atataaacta agtaattaaa
      781 taacattttt aggttggaaa aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa
      841 acgcaaacgc tgcggaataa gaaaaatact gataactcaa cgtttagcag actatctaat
      901 agggatagtc tgcttttttc gtttttgacc atacttttga ccttctgcaa acttccttgc
      961 ttttcctctg tagaactcat gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt
     1021 ttcgcgatat gggtgtatat atcatccata gtggttttaa tatctgtatg tccaagtcga
     1081 acatacactt ctttgacaga cgcaccagat tcaaagagca gactacaatg ggtatgacga
     1141 aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa tgatgatttt caaattgtga
     1201 tttacatagt ctaaataaag gggcttattt tcttctgatg aaaacaaaag ttgttcacca
     1261 tcttttatag gtttgattct catttttaga tattatgccc gcacgtattc atcacatttc
     1321 tattgtgaat cgctttattc gaccgacatt tgacttttat cataatatac tcggtttaaa
     1381 attactaatg aaaacaatta atcaagacga tcataccatg tatcacctgt ttttttcaga
     1441 taatcaccac cgtgtgggaa ctgaactgac tttttttgaa gtacaagaag ggaacaacca
     1501 gttttttgga accaatacga ttgaacgaac gatactaaaa gttccttccg aagcatcatt
     1561 gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt
     1621 ttctggacga cctatcttac gttttgaagg gccagatgca acacgattag ctttagttcc
     1681 cttacgcgaa tttgaagata ttgatgatta tttcccgtat gtcacagacc agatccctac
     1741 agagcatgcc attttaggaa ttgatgccat tcaactacgc gtacaatatt ccgatgcgac
     1801 tgaacgagag ttattgtcgg taggctggta gcacaaagaa tacgtcagtt tcttttcggg
     1861 tcatcaacga gtgacaatct tagaaaatca agacactcag ttctatcaag aagttcagat
     1921 tattcaagat cgtgagaatc cagttgccat tgaagggatt ggtagcgtcc atcatgttgc
     1981 gtttggcgta gaaaataaat ctgatttaca acgcatcgac aaacaattac aagagcgcaa
     2041 ttttataaat tcaggaataa aagatcgtga attttttgta tcactttatt atcgtgaccc
     2101 aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga
     2161 aaatcaaagt ccacgctttg aagaaattcc tttattttta ccgcagtact taaatttcat
     2221 tcgtgaacaa gtggagcaat aattagctaa tcaaatccat gacgaagagt aagaggttga
     2281 ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat tctaaagaat gttgatttga
     2341 tgcggttcct atttttataa actatcattt tcgactaata aaactactta tgagtcagcc
     2401 tctctttttt tcttgatttt tatacctctt tctaggtaaa ctaaacatct gccaaaaata
     2461 ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa ggggagtacc aaatgaacga
     2521 gtatattgaa attatccacg caagagaaac aacttaaaaa tgtcagtctc aaaattccca
     2581 aaaataaaat tagtattttt accagcgtat ctggttcagg aaaatcatcg attgtttttg
     2641 atactattac ccaagaagct ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt
     2701 tatttttacc taaatacagt cgtccagagg ttgatgagat tgataatctt tctacggcga
     2761 ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc aacgttaggg acgattacta
     2821 acatcaatgc gttatttcgt gtgttgtttt catgttttgc aacacctagt ttaggatatg
     2881 caaatgctta ttcatttaat gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta
     2941 agacgattgc atttttaata ggattgcttt atttttcggc tattgataat gctgattttg
     3001 tattttctga tccatttacc gcagaagttt tgtctggaat tgaggaacag gcaaagaatc
     3061 atggttattt tgtattggtg cataatgtta attcagcaga agatgttaaa actattcaga
     3121 gaaattggcg ttttgagggg ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat
     3181 taaataacgt ttttgatggt cctgttgttt atattgatac gcatctttca aaagagacat
     3241 taagagaact tgaacaacaa atgaatcgtt tatttgtaaa tacaaatgat taccaagcta
     3301 gtacattggc agtggaacat ttgattcatt atggtcatga gaaaatagcg tttctttctt
     3361 ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg acatcaagct tatatcgata
     3421 cattaaagaa atatcattta acctttaaat cgcattggat atataacaac tctgaatttg
     3481 agcgaattta tcaagaatta gctgaattta ctgcaattgt tgttactgga gattatttag
     3541 cggctaaatt agtaaaattt ttaaaaaata agaataaacg agtgatggaa caattatcga
     3601 ttattggatt tgatgatatt acttttgcag aattaatgga tcctgcgttg acaacaatac
     3661 gactaaatcc aggtaacaag ggggcgattg cttttcacca actgattgga ctggccaaac
     3721 aagaagaaat aaaaaatcaa gtactactat tagagggaga attgatttct cgagactctg
     3781 ttcacaaaat caaataaaaa agctctgtga aataagtata gttaacaata aaatatccga
     3841 acgatagaga gaatgaactg tgatctagca gttcctcgtc gcaaattcac tagaattagc
     3901 attatttact ctaaattgtt cggatatttt ttcttgtttt taattatgat tacagtgcct
     3961 atttttaacg aaatttgctt attttcaaaa tgtatgatta tatgttagta aacactttca
     4021 aatatgaaaa gaggagctat tatggtagga attagggatg ttgcaaaacg ggcgggtgtg
     4081 tccattgcaa cagtatcgcg cgtgctaaat gaggatccga cactttcagt aaccgatgaa
     4141 accaaacgaa agattgcaga atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt
     4201 gcaaaaaatt taacaaatat tgcattaata acgacagttt ctgaagtaga tgagttggaa
     4261 gatccctatt ttcgaacgat tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc
     4321 aatttgaaga aaattattcg tttgtcagaa acaccgttgg agcaagacga tttgctaaaa
     4381 tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg tgattgaaga agtatcagca
     4441 attaattcga atgtcgtagt gattgatgac cctagtattg aagctgaagt agatgcagtt
     4501 tatactgatt taacaaaagc aacgaatcag catttagatc gattgtataa aaaaggacac
     4561 cgaaacattg cgtttatcgg aggaggacgt gttcgtttag atagccaagg aaataaacac
     4621 gtgtcagatg atgattatcg aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta
     4681 gaaaaatata gtcaaattta tttagaaggc tggaccacac ttgacggaat gcaggcagca
     4741 gatttacttt tgaaaaaaca tgctaaagat aagttgccga ctgctatcat ggtaggaaat
     4801 gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa atggtctaca tattccagaa
     4861 gatatttcga ttgtgagttt tgataatatt gaagttgctg aatttttaac acctgcatta
     4921 aatacggtga atgtgaatac agaagaaatt ggaaaactag cagttcgcat ggttaatgaa
     4981 cgaataaaag aagttagaaa tgtttcgatc aaagtgattg tttctaataa tattattgtt
     5041 agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt
     5101 tttgtttact aatattttta gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt
     5161 gttgttaaca aaaagaaaac gcattcaaaa caggaggaat taagatgaaa catttcaaaa
     5221 aaatagcttt aacagggata gctgttttaa ctgggacatt actcgcagca tgtggtgggg
     5281 gagatagtaa ttctaaagac gggcaagtaa ctttaagcta tgcgagttga gatactgacc
     5341 aagctccagg actaagaaaa atgttagatg aatttgaaaa acaaaatcct gatatcaaag
     5401 tagaaatgga gacaacgcct tgggatcaat attgggttaa attagaagct gcaacaacgg
     5461 gaggtaatat gccagatgta gtaaccatgc actctagcga atcctataaa tatatgtcac
     5521 agaatgcttt gatgggactt gatgatgtta tcaaagaaaa tgatattgac atgggaaact
     5581 ttactgaagg aattgctgat ttctatacgc ttgaagacga actatacgca atccctaaag
     5641 atgcttctgt agtggggcta tggtacaaca aagaattatt tgataatgca ggtgtcgatt
     5701 atccagatga aacatggaca tgggaaacac tcagacaagc tgcaattgat ttaacagatg
     5761 aatcaaaagg catctatggt tttgcatcac atattggaac agagactggc tactggccat
     5821 ttatttatca aaatggggga gaagtatttg ccgatgacaa tacaaaatca ggtttgaata
     5881 ccccagaagc acaagaagct ttagcttttt atcgagatat tgcacttgtt gatcatgctt
     5941 caccaacaat tacagaatta caagatcctg ataagttaag tcgttttcaa tcaggtagat
     6001 tagcaatgat gattgaagga aactggtttg caccgggatt tttgaaaaat gactatctag
     6061 ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt taatgcaaca attacaaatg
     6121 gacttggttg ggctgccagt gcgagtacaa aatacccaga agaagtgaaa aaactaatgg
     6181 catttttagc tagtgaggac gccaataaaa tccaagcgga aacgggggca tcaatcccag
     6241 cactaaaagg ctttgaagac gagtgggctg cttcttcaga tacaattgat ttgagtgctt
     6301 ttggaaaaat gttggagtat ggagtccctc gtccatacaa taaagatgga ttacgagctg
     6361 aacaagcaga agttcaaatt attaaccgta ttctgtctgg tgaagtaact gttgaagatg
     6421 gaaccgctca aattgaaaaa gaagtcaatg cgatattgag cggaaattaa attagttaat
     6481 tatacaatat acaggcgaaa ggattctatt tcgtctgtat attaatggat gaataccaaa
     6541 aagggggata ttatgaaaaa tcgaattttt ggaaaatatc gtaaagatca agtgatatgg
     6601 ggctatattt ttatttttcc aacggcatta ggattaattt tattaaatat atggccagca
     6661 attcaaacag caattttaag ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc
     6721 ggattaagaa attaccaaaa aatgtttgca gacggtgaag tttttcagtc tttattaaac
     6781 acattaattt atgcagtggt ttcgatacct agtattgttg tattatcctt gttggtggca
     6841 gttttaatga atagaaaagt taaaggattg tcgatttatc ggacaattta ttttctacca
     6901 gtcgttgctg caccatccgc agtcgcaatg atttggcgct ggatgttcaa taatgattat
     6961 ggactgatta ataacatgtt ggctaaaata ggtctcgatg gaccagcatg gttaacggat
     7021 ccatcgattg cgatttattc tattatcatt gtaggagttt ggagtgctat tggttataac
     7081 atggtgttat tactagccgg cttataagaa attcctaaag attattatga agcagcggaa
     7141 attgatggtg ctagtccaat ccagcagttt ttcaacatta cgttaccatt agttacacca
     7201 acattatttt ttgtagttgt aacaacagtt atcaatgctt tccaagtatt tgatgttatt
     7261 tttatgatga ttactcctaa tagtaccgcc atgtttaaat cacaatcatt agcctattta
     7321 ttctataaac attcatttat tttaaatgac aagggatatg gttcggcaat cgttatgttc
     7381 cttttagtga ttatattaat tattactgca attcaaatga agttgcagaa aaagtgggta
     7441 aactattaag gaggctatca aattatgaaa aataaatttt tttcaaatgc gccaatccat
     7501 atcatgctta ttttaggtgg tattgcaatg gcattacctt ttatttggat gattttaact
     7561 tcagggaaaa cgttggttga atctacccaa attccaccta caattttccc tgagaaattt
     7621 cagctaagta actatcaaca agtacttgat ttattaccat tcatggcttt ttatatcaat
     7681 acatttttaa tggttgttgg acgagtgatt ggctcggttt tattttcagc aatggctgca
     7741 tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc ttttcagctt agtacttatt
     7801 caaatgatga ttcccggttc catttttatt actccacagt atttattagc acaaaaattg
     7861 ggtattttaa atacaattcc agcattaata ttaccagggg tagttagtgc gtttggtacg
     7921 tttttattaa gacaattttt cttaggttta cctgatgaat tggaggaagc ggcctattta
     7981 gaaggtgcca atatttggac gatctttttc aaaattatgt tgccgcttgc taaatcagga
     8041 ttggttgcac ttggaatatt cacagcgttg tttgcattta aagatttaat gtggccattg
     8101 attgtcaata cttcaattga taagatgcct ttagctccgg gattagcaaa cttacaagga
     8161 caatatgcca cgaacttccc acaattaatg gcaggttcgt tattagcaat ttggccaata
     8221 gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag gaattgctac aacaggtagt
     8281 aaattataaa actattaatt agtgattgga gagtaagtac atgacaaaaa ttacatttat
     8341 tggtgcagga agtactattt ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag
     8401 tctacaagat gcacatattg cattgtacga tattgatgaa aaacgactaa aagaatcaaa
     8461 tttaatgctt aaaaatatta ataaaaattc gaatgaaaat cgtgctgata ttgtttctta
     8521 ttatgatcgc aaagaagcgt taagagaggc tgattttgtt atcaatgcta ttcaagtggg
     8581 tggttataaa ccttcaacag tgattgattt tgatattcct aaaaaatatg ggttgcgaca
     8641 aactattggt gacaccatgg ggattggtgg gatttttaga accctacgga cactaccagt
     8701 aatgttagac tttgctaaag aaatggaaga agtttgtcca aatgcttggt tattgaacta
     8761 tacaaatccg atggcgatgt taaccatggg tgttttaaaa gcgacgagta ttaagactgt
     8821 tggtttatgt catagcgttc aagtatgcgt accagaattg tttgagcatt taggaatagc
     8881 cgatcaatat gacttaaatg actttcaatg gaaaattgca ggcattaatc atatggcatt
     8941 cttgttggaa attacaaaaa atggggaaga tttctatcca acaattcgtc agttagctgc
     9001 agaaaaacca aatccacatc cagattctgt acgttttgaa ttgttgaaac gttttggtta
     9061 ctatattaca gaatcaagtg aacacaatgc agaataccat ccatacttta ttaaatcaaa
     9121 atacccagaa ttgattgacg aattagggat tccgttggat gaatacttac gtcgttgtga
     9181 acaacaaatt aatgattggg aaagcatgcg agaggacatt gtaaataatg gggagttaac
     9241 acatacgaga agccgtgaat atgcttcata tatcatggat tctattacga caggaactcc
     9301 gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc acaaacttac cgacagatgt
     9361 ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg caaccaacct atgtcggtga
     9421 attgccaaca caattagctg ctttaaatcg gacgaatatc aatgttcaag aactgacagt
     9481 agaagccgct ttaacactag ataaatcaaa aatttatcaa gcagcgtatt tagatccaca
     9541 tctatcatct gaattgtctt taacagatat caaaaattta gtggatgatt taatcgaagc
     9601 acatggggac tatttacctg aatataatta aaaagaagct gaaagagtaa ggaattttca
     9661 attccttact cttttcttag ttaaatttag taaaaatatt gacaaaacac ttagtaaaca
     9721 gttacattag tgttaacaaa aagaaaacgc attcaaattg tataaggagg agcataaaat
     9781 gaagtggtct aaaaaaatta ttttagcttg tatagcacta ggagcaacga tctctttgac
     9841 tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta gaaatagagt tttttaatca
     9901 aaaaaaagaa atgactcaaa cgattcagga gattgctaaa gattttgaag caaaaaaccc
     9961 tgatattcat gtaaaagttg tggatgtacc gaatgctggt gaagtaatta aaacacgtat
    10021 gcttgcaggc gatgtaccag atgtcattaa tctttatcct caaagtatag aattgaaaga
    10081 atgggcgaaa gctggttatt tagaggactt aaccgaagaa ccgtatttag agaatatcaa
    10141 aaatggatat gcacaacgtt ttgctattga ggatcgtgta tatagcatac cattaaccgc
    10201 taatgtctat ggtttttact ataacaaaac tgcttttgag gaaatgggga ttcaagcacc
    10261 agaaacttgg gcagaatttg aaaaaatagt agctgagatt aaagatcaaa acaaagtacc
    10321 gtttgcgatt gcaggagcag aaggctggac tttaaatggt tatcaccaac tagctatagc
    10381 gactgctgct ggtggtgaag aggaagcgaa caatgtttgg cgtttctcag aagttaatgg
    10441 aattaatgct gaaagcaaag aaatgcaaaa agattttaat cgattagatt tgttaagaga
    10501 accaaaagct ttgcaaaaca actggcaagg tgccggttat aacgatacag tagttacttt
    10561 tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg gcaatgccga tgattcattc
    10621 acaaaatcca gattttgaag taggaacttt tccttttcca gcagatgaag caggtcaaag
    10681 tctaacgatt ggcgcaggag acctagcctt atcagtttct gcaacttctc aacataaaga
    10741 agaagccaaa cgttttgtag aatatatgac aacacctgaa gcgatgcaga aatattatga
    10801 tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag aatactcagg attcaccatt
    10861 aagtggttta actgaacttg cttttactga tcgtcattta gtttggttag ccaaagattg
    10921 gaatagtgaa aatgattttt ataccttaac aacaaattat ttacacaatg gcaatcaaaa
    10981 agccatgatt aacgcattaa atgctttctt taatccaatg aaagctgacg gaaggtagga
    11041 aaataaaatg aaaataaaag aaattttaaa tcgttactgg gcaatcattt ttgtagcaat
    11101 tccaattatt ttgcagatga tttttttcta ctttccaatg gttcaaggtg ctttttacag
    11161 tttaactaat tggactggtc tgacttataa ttttgatttt gtgggtttga acaattataa
    11221 gattcttttt tctgatccga agtttatcaa atcgattggc ttcactttaa ttgtgacgct
    11281 ttgcatgatt gctggacaaa ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa
    11341 tattaaaggg aaaacattct ttagagcatg gttctttttt ccagcggtct tatcgggcgt
    11401 ggttgtttct ttagtattta aacaaatttt taactatggt ttaccaaaaa ttggtagtat
    11461 gttaggcatt gaatttttac aaaccagtct tttaggaaca acatggggag cgatttttgc
    11521 agtaattttt gttttacttt ggcaaggaat tgccatgcca gtcatcattt tcttagcggg
    11581 ttttcaaagt attccacaag aaattattga agctgctaga atggatgggg cgaatgataa
    11641 gcaaattttt tggaatattg aaatcaccta cttattacca agtatttcaa tggtatttat
    11701 tatggcatta aaaggtggct taacagcatt tgatcaaatt tttgctttaa ctggtggtgg
    11761 accaagcgat gccacaacat ctttggggtt acttgtttat aattatgctt ttaaaaacaa
    11821 tcagtttggt tatgcgaatg ctattgcttt agtcttattt atcttaattg ggattgtttc
    11881 actggcacaa ttaaaattat cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca
    11941 tcaaaagaaa aaaatatttc taaatacttg cttttattat tagggggctt catgatttta
    12001 gttcccttag tttcaacgct attcggttca tttaaaacaa cacccgatat tatgaataat
    12061 ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc gaagattgtt agcggatgga
    12121 attggtcatt acttttggaa ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca
    12181 gcaattattc ctatggctgc ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac
    12241 ggattgatgt atttattttt aattttagga atttttgttc ctttccaagt aattatgatt
    12301 ccgattaccg ttatgatgag taaaattggt ttagcaaata tgtggggatt aatcttactc
    12361 tatctcactt atgccgtacc acagacacta tttttatatg ttggttttat taaactaaat
    12421 gtgcctatca gtctggatga ggctgctgaa attgatgggg ctagtcactt tacaacttat
    12481 ttcaaaattg ttttcccaat gttaaaacca atgcatgcga cgactattat tcttaatgct
    12541 ctatggtttt ggaatgattt catgctacca ttattaattt taaatcgtga ttctaatatg
    12601 tggacactac cattgtttca atataattat acgggacaat attttaacga ctatggtcca
    12661 agttttgctt cctatacagt aggaattgtc acgattacac tagtttatct tgctttccaa
    12721 agaagtatta tagctggaat gagcaatgga gcagtgaaat aaccaataaa ttattctcat
    12781 tataaaggag ttcataaact tatgattata tttgacaatc acacacaaac atttcattta
    12841 caaaataaaa aaattagcta tctattagga atcgaagaaa ataattattt aacgcatctt
    12901 tattttggaa aaaagattac gcactattct ggtgggtatc gctacccccg aacagatcgt
    12961 tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat tatctttaga cacattacca
    13021 ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa tattaaatta
    13081 ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca aaaaggaaag
    13141 cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc tgaaacgctg
    13201 attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta cacaatttat
    13261 caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga tgaaacagtg
    13321 gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact cgagttaatt
    13381 catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat tcatacaggg
    13441 ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc gtttgttgct
    13501 ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa tttagtttat
    13561 agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg ggtcattatg
    13621 ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca
    13681 gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac gtatcatgaa
    13741 ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc
    13801 aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat
    13861 gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt tggcggtcgt
    13921 gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact tgcttgtggt
    13981 ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat ttggtttgaa
    14041 ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg ggcattgggt
    14101 attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt ttcaagacaa
    14161 gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt accgattgat
    14221 tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt gttggactca
    14281 acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt tttagaaaag
    14341 ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg cggtcgtttt
    14401 gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac cgatgccgtg
    14461 gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag tatgggtgcg
    14521 catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc
    14581 aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt aacttctgaa
    14641 gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca acttttgcaa
    14701 tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc atggatgttt
    14761 gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc agaagctgcg
    14821 catccattag tcacattgaa attagcagga ttagcagaag aagcaactta tcaaatggaa
    14881 gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga ctcagaacta
    14941 catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct tgaatttaca
    15001 atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt ttatttacac
    15061 gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc
    15121 tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat cgatgcgata
    15181 ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga agatgggtta
    15241 gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg
    15301 agagagcgga catttaaacc acataatagt atgacggtag cagaagccga tgttcctaat
    15361 gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt tgattttagt
    15421 tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca atggacatgg
    15481 acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa aggttggggc
    15541 gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat tccagaagaa
    15601 tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact aagaggtacc
    15661 ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc atcattagaa
    15721 gattttgatg atgtagctac gcattctcag tataaacggg cattagaata tggattggca
    15781 ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg cacacctatg
    15841 caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg gttaaaagta
    15901 aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca gtcggtattc
    15961 aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga cgtgctaatc
    16021 tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta tgaacgccaa
    16081 ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca agaatattat
    16141 gtagatgaac agtaccaaca agtattaatc aataattatg aagatgtttt attagatcaa
    16201 agtcgaaaat tgcgtttacg accgtttgaa agtattgtat tagctaatta tggaggacaa
    16261 ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc agatagttta ggaaaaaaca
    16321 tgggcgaatt aaaacaagtc ttagacaaac atttacaagg tgttattggt ggtgtacatc
    16381 tgttgccttt ttttccatcg acaggtgatc gaggctttgc gccgagtgat tatacgacag
    16441 ttgatccagc tcttggaaca tgggaagatg tggaagcatt aggtgaagat tattatttga
    16501 tgtttgattt tatgattaat cacatttctc gtgaatcaaa atttttccaa gatttcaaaa
    16561 aaaaccatga gcagtcacca tacaaagaaa tgtttatccg tattcatgaa tttttcccag
    16621 aaaatcgacc aacacaagaa gatattgatt taatctacaa gcgtaaagac aaagcgccgt
    16681 ttcaaacggt agaatttgcg gatggtacga cagaagaggt ttggaatacc tttggggaag
    16741 aacaaattga tttagatgtg accaaagaag ttgtgaaaga atttattcga gaaaccatca
    16801 aagatatggc gaaccatggc tgttctttaa ttcgattaga tgcgtttgct tatgcgatta
    16861 aaaaattaga taccaatgat ttcttcgtgg aaccagatat ttgggaactt ttagacgaag
    16921 tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc agaaatccat gagcattatt
    16981 ccattcaaat gaaaattgcc aatcatgatt attatatcta tgacttcgcc ttgccaatgg
    17041 tcactctgta ttcactttat agtggaaaat cagaacgttt agccaattgg ttgaaaatga
    17101 gtccgatgaa gcagtttaca acattggata cgcatgatgg aattggtgtt gtggacgctc
    17161 gtgatttatt aacagatgaa gaattggatt acacctctga ggaattgtat aaagtcgggg
    17221 cgaatgtgaa gaaggtctat tcaagtgcca attacaataa tttagatatt tatcaaatta
    17281 acagcaccta ttatagtgca ttaggcgata atgaccgtag ctatctgtta gcacgtgcga
    17341 ttcaagtatt tgcgccaggg attccacaaa tttattacgt tgggttatta gcaggaaaaa
    17401 atgatattga actgttggaa gcaacgaaag aagggcgtaa tattaatcgt cattactatg
    17461 atttagccga aattgaagaa gaagtgcaac ggccagtgat tcaacaatta tttgcacttt
    17521 taaaatttag aaatgaatct gcggcgtttg atttggatgg aacgattgaa gtagcaacgc
    17581 catcagaatc agaaatagtg attacacgta aaaataaaaa cggtagtgat agtgcaacat
    17641 taacggctaa cttggatacg aaagaattta tcattgagtc taaagatata aaaattttca
    17701 ctagtgttgc attaaaattt taacctattc catagaacaa aaaatccttt atactagcac
    17761 ttggatgact tatccaagac caataaaaag gactcataca tggataagta taaaacaaat
    17821 acagcattta ataaatggtt ttctgccata aaattaggaa aactttctgt caactcccaa
    17881 aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag
    17941 ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt
    18001 atcgaccgat cgctccaact agaaaccgat gtgtcatcga ttagtcatac ccaattgtct
    18061 cgagcattta atgcacttga acctaaagtg ttagaagaaa tttttcaaca actgttagaa
    18121 aaagttcaac atcaaatcaa accaaccaag cgaaatcatc tgtatctgat tgattccagt
    18181 acgttttcgc ttagtttaaa acgtcataag tgggcgactt tccgtcaaac aaaatcaggt
    18241 gtcaagttac acttaaacta ttgttatatg gacgatacta cgatgtatcc aactgatttt
    18301 accttaacaa atgcccatga acatgatctc aatcaactac cagtattggt gaatcaacca
    18361 gaggcaactt acgtgtttga tcgtgggtat taggactttg aaaagatgga tcagatgcat
    18421 tgggatggct atttcttcgt cactcggatt aaaaaaaaca ccaaagttca cgtgatagat
    18481 actctggaaa catcgccaga aacagaaatt ctacgagacg agctggttcg tttaggatct
    18541 aaaacctatc taaccgccaa tttccgacta gtcactgttc aagataagaa cggacgtgtg
    18601 tttcaattta tcaccaatcg aatggacgtc tcttcaaaag aaatctctga tatgtatcac
    18661 gctcgttggc aaatcgaatt gtttttcaag cacatcaaac aacacatgac cattaaaacc
    18721 ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga ttttaacgat gatttccgct
    18781 ctcctcacgt ttctcatcaa attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa
    18841 cgattctttc gttatttact gtttcagccc tttgaatgtt gtctagaaaa attaattccc
    18901 acatgagaaa aaagaaattt ggctcgttac ttttggtttt cagttgaata acagataaaa
    18961 aattttctgg aaaaagtcat cctctgcgtc agcacagttt tttttgactt attttcagag
    19021 gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat
    19081 tagatttcta atgtgcattt tttgagtatt aaggagcaag tatacaaaga tttataaagc
    19141 tgattaaatt tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta
    19201 tatttgaata aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa
    19261 ttaggttatg ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggtt
    19321 tcaaaaagct atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta
    19381 gaagaagttt taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt
    19441 taaaaaagat aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac
    19501 agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt
    19561 ataccgaact tgtttctaat tatggaaaag aagcagaaac ttctcaaagt actatgttgt
    19621 ttgaggagtt tttttataaa ttattcctac cttattatca agggaaagta gaagaaagaa
    19681 catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta aaatggagtt
    19741 aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga gcaaatataa
    19801 aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc gagcagttgt
    19861 tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga aaaggaaaac
    19921 tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt tttatattga
    19981 tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga ccgttatgcg
    20041 tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata aaaacttgcg
    20101 tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag aacccgaaac
    20161 ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt taaaagcttg
    20221 gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata atggaacacc
    20281 tactcagaaa tatacaatcc gtcatattat gagaagacat gcaacgatcg ttggtgttca
    20341 tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat cacaataaaa
    20401 agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga ctttaggaac
    20461 ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca cagattatat
    20521 ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa aataaatgat
    20581 aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata caggcttttc
    20641 tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc atttaatgca
    20701 tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt acttgaatgt
    20761 cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct agtagctaat
    20821 gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata ttttgctacc
    20881 atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat tgctagaaat
    20941 aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag ataattagct
    21001 aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc ttttctagtt
    21061 actaccacat tattcttatc taaagatagg tcatctaaat taatatttat aacctcggag
    21121 agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc tacatctcgt
    21181 tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc aatataatct
    21241 agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt ggcacgtgca
    21301 gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt gtctaagtat
    21361 ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgcc
    21421 gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc tttgagctta
    21481 gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg agatttgtac
    21541 aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc aggaacgata
    21601 gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc taaagtggtt
    21661 cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata
    21721 tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc attcatcctt
    21781 tcaataagta ggggagaaga gtat
//
LOCUS       DACDBG010000042        21801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDBG010000042 DACDBG010000000
VERSION     DACDBG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.042"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..884
                     /locus_tag="IVZ90_001607"
     CDS             <1..884
                     /locus_tag="IVZ90_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8262657.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="IVZ90_001608"
     CDS             932..3061
                     /locus_tag="IVZ90_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8262658.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="IVZ90_001609"
     CDS             3036..3725
                     /locus_tag="IVZ90_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262659.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="IVZ90_001610"
     CDS             complement(4133..4819)
                     /locus_tag="IVZ90_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8262660.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="IVZ90_001611"
     CDS             complement(4955..5149)
                     /locus_tag="IVZ90_001611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262661.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="IVZ90_001612"
     CDS             complement(5199..5639)
                     /locus_tag="IVZ90_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8262662.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="IVZ90_001613"
     CDS             complement(5643..6488)
                     /locus_tag="IVZ90_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8262663.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="IVZ90_001614"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="IVZ90_001614"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8262664.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8131..9333)
                     /locus_tag="IVZ90_001615"
     CDS             complement(8131..9333)
                     /locus_tag="IVZ90_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262665.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9320..11107)
                     /locus_tag="IVZ90_001616"
     CDS             complement(9320..11107)
                     /locus_tag="IVZ90_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262666.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11120..12073)
                     /locus_tag="IVZ90_001617"
     CDS             complement(11120..12073)
                     /locus_tag="IVZ90_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8262667.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(12258..12608)
                     /locus_tag="IVZ90_001618"
     CDS             complement(12258..12608)
                     /locus_tag="IVZ90_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8262668.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            12808..13887
                     /gene="pepA"
                     /locus_tag="IVZ90_001619"
     CDS             12808..13887
                     /gene="pepA"
                     /locus_tag="IVZ90_001619"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8262669.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14030..14350
                     /locus_tag="IVZ90_001620"
     CDS             14030..14350
                     /locus_tag="IVZ90_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8262670.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            14372..14836
                     /locus_tag="IVZ90_001621"
     CDS             14372..14836
                     /locus_tag="IVZ90_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8262671.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            15032..15637
                     /locus_tag="IVZ90_001622"
     CDS             15032..15637
                     /locus_tag="IVZ90_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8262672.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(15676..16965)
                     /locus_tag="IVZ90_001623"
     CDS             complement(15676..16965)
                     /locus_tag="IVZ90_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8262673.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            17393..17872
                     /gene="rlmH"
                     /locus_tag="IVZ90_001624"
     CDS             17393..17872
                     /gene="rlmH"
                     /locus_tag="IVZ90_001624"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8262674.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            18225..18539
                     /locus_tag="IVZ90_001625"
     CDS             18225..18539
                     /locus_tag="IVZ90_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8262675.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            18555..18941
                     /locus_tag="IVZ90_001626"
     CDS             18555..18941
                     /locus_tag="IVZ90_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8262676.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            18970..20355
                     /locus_tag="IVZ90_001627"
     CDS             18970..20355
                     /locus_tag="IVZ90_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8262677.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            20358..20511
                     /locus_tag="IVZ90_001628"
                     /pseudo
     CDS             20358..20511
                     /locus_tag="IVZ90_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            20534..21739
                     /locus_tag="IVZ90_001629"
     CDS             20534..21739
                     /locus_tag="IVZ90_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8262678.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         7067 a         4035 c         4403 g         6296 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat
     8101 gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt
     8161 ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt
     8221 acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata
     8281 cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg
     8341 tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac
     8401 ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt
     8461 gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt
     8521 caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca
     8581 aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat
     8641 taaatactta tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg
     8701 tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa
     8761 ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt
     8821 caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc
     8881 aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct
     8941 cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact
     9001 catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc
     9061 cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat
     9121 catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga
     9181 aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga
     9241 gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac
     9301 aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt
     9361 tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa
     9421 caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa
     9481 gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg
     9541 cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga
     9601 tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcataccat
     9661 aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc
     9721 agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat
     9781 ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat
     9841 ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg
     9901 ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag
     9961 ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat
    10021 aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt
    10081 gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc
    10141 cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta
    10201 cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta
    10261 aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga
    10321 ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga
    10381 tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt
    10441 gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa
    10501 atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca
    10561 tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca
    10621 gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag
    10681 agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa
    10741 tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa
    10801 tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct
    10861 gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg
    10921 caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa
    10981 ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa
    11041 aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct
    11101 tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt
    11161 ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg
    11221 ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc
    11281 gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa
    11341 ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca
    11401 ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
    11461 taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta
    11521 aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt
    11581 tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat
    11641 ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat
    11701 tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag
    11761 aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta
    11821 ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca ttcccttttc
    11881 cttgacgtgg ttctttcttt acgattgctc cgccttgaac ggccaattcg tatgttcgat
    11941 cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt
    12001 caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt
    12061 cacgtgattc catttgtcac tctccttata taataaaact aatcttattt caagaatacc
    12121 gtataataac tacaaatact agctattcca agaggaaata gccctatttt tataaaaatc
    12181 acccactata aaagaaggat aggatacctc tcttttagat agtgggtgcg tattcgttat
    12241 ttcatttttt tgacgtatta atctattgcg cccttttttc ttgaaatgtc taatacggtt
    12301 cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc tatccgggaa
    12361 agcccacccc gataagcact cgaccgaacg gcgaatttcc gaataggttc tttttcaaaa
    12421 gagatccaag caccttcgat tgcgccttct tttaaaaaag atgttttgat attttcaagc
    12481 acttggtctg ctgataacac ttttttgcga tgataccaaa tcgttgaagc taccccactc
    12541 aataatccta gaccgatccc aacggctaac ccgcctttaa aatagcttaa ttcgctttct
    12601 tcatacatgt ggatatacct ccttcttctc acttgctact ctttgtgttt attttaccac
    12661 actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata
    12721 cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt
    12781 ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag
    12841 ttaacagaat tacaaggaac aagcggtttt gaacaagacg ttcgcgccta tatggaaagt
    12901 catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttgggctg
    12961 cgccaccatg aagaagcgga tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc
    13021 ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcgga
    13081 tggaaccctt acgttgtttc tgctcaacgt tttacactta aaacctctac tggtaagaat
    13141 tatccatgta tttcttcttc cgttccccca catttattac gaggaacaag cggacaaaaa
    13201 tcagtagaag taacagatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg
    13261 agttacggtg ttcttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc
    13321 aacgggaaga atatcatcag caaatcgtgg gacaaccgct atggctgtac aatggtatta
    13381 gaagctttag aagcgttaca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat
    13441 gtccaagaag aagtaggctt gcgtggttct aaagcttctg tcaataaatt caagccagat
    13501 ctgttctttg cagtagactg ctctgcagca gatgacacag tgacgaaaaa tggcacattc
    13561 ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct
    13621 cgtttgcgtg agtacttgct agatatcgct gaaacaaaca acatccctta tcaatacttt
    13681 gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaatgaagg aatcccaagt
    13741 acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc
    13801 gattttgaag ctgcacgaga aatgttgatt caaacattga aaggtttaga taaatcaaca
    13861 gtgaacacga tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc
    13921 aaccttttgt cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag
    13981 gtgtataatt taattgaaaa atacataaac agaaagtaga ggaaaaagaa tgattatccc
    14041 aaatagttta gaagaattag caggctacgt agaaactgga aaaagtgtat ttttctttac
    14101 tgccggctgg tgcggagact gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga
    14161 cttttctgat tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga
    14221 atggaacatt tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg
    14281 tctagtgaat aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat
    14341 aaaaggctga catctgttag gaggggtcaa gatgaatcaa gaatataaga acattttagt
    14401 aggtattgac ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag aagtggctcg
    14461 ccgtaataat gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataattttat
    14521 gggctatgct cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaagcaaat
    14581 gatcgaagaa tgtaaagaat atggaaaatc tgttgattac acaaatatag aaggaattat
    14641 tgcatacggc tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat atgcaatcga
    14701 tctaatcatg gttgggcagt ctggactaaa tgcagtcgaa cgctttatga caggaagtgt
    14761 agccagctac gtcatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga
    14821 acagccgagt aattaagcaa gtggaacttg tataaccttt ttaatcaaac agtaaaggga
    14881 ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaagct
    14941 tcttcggaaa taaagcatga aaaaaatctc atgcagtgaa tgcaattttt tgttaagata
    15001 aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt
    15061 cggagacact ttgatggtta tcatagccga cgataaagga acagaaaaca ctgtcgaacg
    15121 taaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt
    15181 ccatatctcc gatcatctaa caattgaagg gaacggccaa gtaagcttaa cagatgaaga
    15241 attagcgaaa ttgaatcgat tgattaaaga agcaggattc gaagaacagc ttgaagctga
    15301 tcatgaaccg aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga
    15361 ccatttatcg attactgaga cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg
    15421 tgcaccaaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc
    15481 agatggaatg atgatctggc caggaacttt gcgaggggta gccagctatg gaatgatttg
    15541 ttctgctcgt gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc
    15601 tgaggatgcc gtagtcggag aagtatttcc aaaataaaga ggttgtgaca agtctttgtc
    15661 acaacctctt ttttattaat tttcactatt ttgattttgt tttaatgccg attgatcgat
    15721 cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga
    15781 atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt
    15841 gtcatcgatc ttagtaatga cgtcgtattg tttcaaccca gctttttctg ccggtgtagc
    15901 tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg
    15961 ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg
    16021 tgtcactttt ccgtcttttt ccagttggtt gatgatattc actacatcgt tacttgggat
    16081 ggcaaatccc attccttcta cgcttacatt agaagtcgat tcagaagtac ttgcgatctt
    16141 acttgagttg ataccgataa cttgtccttc gatattcact aaaggaccac cagaatttcc
    16201 cgggttgatc gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt
    16261 cgtacttgtt acttgtcggt tcaaggaaga aatgattcct gaagtgacag aattcgcata
    16321 ttcagatcct aatggggaac caatcgcaat cgcaggttca ccaaccttca aagcacttga
    16381 gtccccaaaa gaggcaaccg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc
    16441 tgaataggca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcatgac
    16501 ttccaaacct tgctgtccat ctactacgtg attgtttgtg actacataag cagtattccc
    16561 gtcttttttg tagattacgc cgcttccttc actataagct tccaaattac tatcatctga
    16621 gctgctactt tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt ggttttgact
    16681 ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc
    16741 agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat tttgattgcc
    16801 gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt
    16861 cagtagtcct ccaacgatcc ctccaacaag ccccagaccc aacttacgcc agaggctatt
    16921 atttttattt tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa
    16981 ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga
    17041 ctttgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga
    17101 ttttcttttt ccacagatat tgtgtaaaac tacgcggata ggattggaaa aataagggat
    17161 aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct
    17221 aatgaatgtg atgaaataag gtaaaacaac gctgttttct gtggataaac ctgtggatgt
    17281 tgtgcataac tgttggggaa atctttttaa caaaaatagt caaaataaac atatagacga
    17341 aaaaattgat tataataaac aaagtgagcg tgaagatcaa aggagagaaa acatgaatat
    17401 caaaattatt tcagtaggaa aattaaaaga aaaatacttg atccaaggta tcaatgagta
    17461 tgtcaaacgg ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc
    17521 cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat
    17581 cttagcgaaa atcaaagaac aagaatacgt ctatggctta gcaattgaag ggaaaaatcc
    17641 cacaagcgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct
    17701 tgtttttgtt atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc
    17761 tcagatctct tttggcaaaa tgacgtatcc acatcaattg atgcgcctga tcctcgtcga
    17821 gcaaatctac cgtgccttcc ggatcaacgc gggggctcca taccacaaat aatgatattt
    17881 tttaagagaa aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact
    17941 ttatcaaggt caaaaccact caatttacta ctaatttact acttatgaat gagctttgat
    18001 acgacgattt atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt
    18061 taataggtag acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag
    18121 tgaacttatg aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat
    18181 caaacattca ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg
    18241 tgattcccaa catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg
    18301 ttcagcgaag aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg
    18361 atgttcaaag agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt
    18421 tcggctttga ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa
    18481 tcggcactcg tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa
    18541 gaaagagagg aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt
    18601 ttggagaatt gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt
    18661 cggtttcaga tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca
    18721 tgattcaagt aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg
    18781 tggaacttat caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg
    18841 ttgactggta tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag
    18901 ctcaaccaca agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga
    18961 ctttttcgca tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt
    19021 cattttacga tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg
    19081 aagacaatcc agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac
    19141 attccctatt taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt
    19201 gtattcaaac gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca
    19261 aagatgatac ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt
    19321 aaagatagtg ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga
    19381 cttaaaaatg gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa
    19441 ctcttacact tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag
    19501 ttaaaggatt cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct
    19561 attgatgaag tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa
    19621 tatgataagc tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt
    19681 atactgacac tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg
    19741 aaaaatgctg accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa
    19801 gacttgcttt cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg
    19861 aagcagatga agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac
    19921 ttcttaatct ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc
    19981 gcagttatga ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta
    20041 atactggctt gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatcagttt
    20101 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
    20161 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
    20221 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
    20281 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
    20341 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
    20401 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
    20461 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
    20521 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
    20581 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
    20641 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
    20701 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
    20761 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
    20821 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
    20881 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
    20941 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
    21001 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
    21061 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
    21121 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
    21181 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
    21241 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
    21301 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
    21361 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
    21421 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
    21481 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
    21541 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
    21601 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
    21661 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
    21721 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
    21781 tatgaatttt ggacaaaacc t
//
LOCUS       DACDBG010000043        20681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDBG010000043 DACDBG010000000
VERSION     DACDBG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.043"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IVZ90_001630"
     CDS             131..505
                     /locus_tag="IVZ90_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8262679.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVZ90_001631"
     CDS             complement(619..1248)
                     /locus_tag="IVZ90_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8262680.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVZ90_001632"
     tRNA            complement(1382..1454)
                     /locus_tag="IVZ90_001632"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVZ90_001633"
     tRNA            1668..1741
                     /locus_tag="IVZ90_001633"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVZ90_001634"
     CDS             1696..1845
                     /locus_tag="IVZ90_001634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262681.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IVZ90_001635"
     CDS             2338..3666
                     /locus_tag="IVZ90_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8262682.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVZ90_001636"
     CDS             3868..4701
                     /locus_tag="IVZ90_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8262683.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVZ90_001637"
     CDS             4717..5454
                     /locus_tag="IVZ90_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8262684.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVZ90_001638"
     CDS             complement(5609..6577)
                     /locus_tag="IVZ90_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8262685.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVZ90_001639"
     CDS             6809..7642
                     /locus_tag="IVZ90_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8262686.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IVZ90_001640"
     CDS             7676..8515
                     /locus_tag="IVZ90_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8262687.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IVZ90_001641"
     CDS             8541..9101
                     /locus_tag="IVZ90_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8262688.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IVZ90_001642"
     CDS             9300..11021
                     /locus_tag="IVZ90_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8262689.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IVZ90_001643"
     CDS             11186..12328
                     /locus_tag="IVZ90_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8262690.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IVZ90_001644"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IVZ90_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8262691.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IVZ90_001645"
     CDS             14128..14775
                     /locus_tag="IVZ90_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8262692.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IVZ90_001646"
     CDS             15168..17813
                     /locus_tag="IVZ90_001646"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8262693.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IVZ90_001647"
     CDS             18123..19436
                     /locus_tag="IVZ90_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8262694.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IVZ90_001648"
     CDS             19426..20079
                     /locus_tag="IVZ90_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8262695.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..>20681
                     /gene="radC"
                     /locus_tag="IVZ90_001649"
     CDS             20134..>20681
                     /gene="radC"
                     /locus_tag="IVZ90_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP8262696.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNV"
BASE COUNT         6973 a         3458 c         4310 g         5940 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg t
//
LOCUS       DACDBG010000044        20552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDBG010000044 DACDBG010000000
VERSION     DACDBG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.044"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..279)
                     /locus_tag="IVZ90_001650"
     CDS             complement(<1..279)
                     /locus_tag="IVZ90_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8262697.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFN"
     gene            complement(304..735)
                     /gene="ruvX"
                     /locus_tag="IVZ90_001651"
     CDS             complement(304..735)
                     /gene="ruvX"
                     /locus_tag="IVZ90_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8262698.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(732..998)
                     /locus_tag="IVZ90_001652"
     CDS             complement(732..998)
                     /locus_tag="IVZ90_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8262699.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1253..1978)
                     /locus_tag="IVZ90_001653"
     CDS             complement(1253..1978)
                     /locus_tag="IVZ90_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8262700.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2339..2409)
                     /locus_tag="IVZ90_001654"
     tRNA            complement(2339..2409)
                     /locus_tag="IVZ90_001654"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2375..2377),aa:Gly,seq:ccc)
     gene            2526..3278
                     /locus_tag="IVZ90_001655"
     CDS             2526..3278
                     /locus_tag="IVZ90_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8262701.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3294..3773
                     /locus_tag="IVZ90_001656"
     CDS             3294..3773
                     /locus_tag="IVZ90_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8262702.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4024..5604)
                     /locus_tag="IVZ90_001657"
     CDS             complement(4024..5604)
                     /locus_tag="IVZ90_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8262703.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTFFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5597..6340)
                     /locus_tag="IVZ90_001658"
     CDS             complement(5597..6340)
                     /locus_tag="IVZ90_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262704.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6516..7292)
                     /locus_tag="IVZ90_001659"
     CDS             complement(6516..7292)
                     /locus_tag="IVZ90_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262705.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7540..8376)
                     /locus_tag="IVZ90_001660"
     CDS             complement(7540..8376)
                     /locus_tag="IVZ90_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8262706.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8387..9316)
                     /locus_tag="IVZ90_001661"
     CDS             complement(8387..9316)
                     /locus_tag="IVZ90_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8262707.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9508..9861
                     /locus_tag="IVZ90_001662"
     CDS             9508..9861
                     /locus_tag="IVZ90_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8262708.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9935..10591)
                     /locus_tag="IVZ90_001663"
     CDS             complement(9935..10591)
                     /locus_tag="IVZ90_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8262709.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10817..12751
                     /locus_tag="IVZ90_001664"
     CDS             10817..12751
                     /locus_tag="IVZ90_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8262710.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12837..14060)
                     /locus_tag="IVZ90_001665"
     CDS             complement(12837..14060)
                     /locus_tag="IVZ90_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8262711.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14054..15952)
                     /locus_tag="IVZ90_001666"
     CDS             complement(14054..15952)
                     /locus_tag="IVZ90_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8262712.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(15946..17064)
                     /locus_tag="IVZ90_001667"
     CDS             complement(15946..17064)
                     /locus_tag="IVZ90_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8262713.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17147..17980)
                     /locus_tag="IVZ90_001668"
     CDS             complement(17147..17980)
                     /locus_tag="IVZ90_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262714.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(17973..18890)
                     /locus_tag="IVZ90_001669"
     CDS             complement(17973..18890)
                     /locus_tag="IVZ90_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8262715.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19117..20241)
                     /locus_tag="IVZ90_001670"
     CDS             complement(19117..20241)
                     /locus_tag="IVZ90_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8262716.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20424..>20552
                     /locus_tag="IVZ90_001671"
     CDS             20424..>20552
                     /locus_tag="IVZ90_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8262717.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESAN"
BASE COUNT         6363 a         4079 c         3407 g         6703 t
ORIGIN      
        1 attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt ctaactcgcc
       61 ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat cttcatcttc
      121 tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt gtccgtcaac
      181 tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg tgatgtgttc
      241 gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc ttttctttta
      301 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca tcacagctgc
      361 taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct ctactagcat
      421 acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca aggagaatag
      481 ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga tggaattgtt
      541 catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta ctaattcctt
      601 caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct ctactccttg
      661 agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac cgacatccaa
      721 gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac aattacttcc
      781 atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata acgaggaatg
      841 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
      901 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
      961 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
     1021 aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga aaactacaaa
     1081 caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt tttccttttt
     1141 tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc tttgaatgta
     1201 tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc tactaatttt
     1261 ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga acattgtaga
     1321 taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat tgcactcccc
     1381 tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca tgatgatttg
     1441 tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga gcattcaacg
     1501 aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc tggaaaccaa
     1561 ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt ttccaacctg
     1621 tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt ccaagcatag
     1681 cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc catgttcctt
     1741 tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt tctatttctt
     1801 gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt gtggaatcaa
     1861 aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa acaccgaatg
     1921 cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt ttgctcattt
     1981 ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat aaataaatac
     2041 tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta aaaatacaaa
     2101 aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt atttattatt
     2161 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
     2221 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
     2281 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
     2341 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
     2401 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
     2461 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
     2521 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
     2581 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
     2641 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
     2701 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
     2761 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
     2821 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
     2881 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
     2941 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
     3001 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
     3061 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
     3121 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
     3181 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
     3241 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
     3301 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
     3361 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
     3421 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
     3481 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
     3541 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
     3601 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
     3661 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
     3721 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
     3781 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
     3841 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
     3901 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
     3961 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
     4021 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
     4081 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
     4141 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
     4201 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
     4261 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
     4321 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
     4381 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
     4441 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
     4501 aataagattg ctgataaaaa aagtctgatt aactgttaag aaagccaatg cgatccctac
     4561 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cacctcctag
     4621 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
     4681 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
     4741 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
     4801 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
     4861 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
     4921 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
     4981 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
     5041 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
     5101 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
     5161 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
     5221 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
     5281 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
     5341 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
     5401 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
     5461 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
     5521 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
     5581 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
     5641 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
     5701 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
     5761 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
     5821 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
     5881 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
     5941 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
     6001 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
     6061 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
     6121 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
     6181 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
     6241 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
     6301 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
     6361 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
     6421 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
     6481 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
     6541 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
     6601 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
     6661 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
     6721 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
     6781 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
     6841 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
     6901 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
     6961 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
     7021 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
     7081 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
     7141 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
     7201 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
     7261 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
     7321 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
     7381 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
     7441 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
     7501 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
     7561 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
     7621 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
     7681 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
     7741 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
     7801 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
     7861 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
     7921 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
     7981 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
     8041 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
     8101 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
     8161 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
     8221 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
     8281 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
     8341 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
     8401 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
     8461 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
     8521 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
     8581 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
     8641 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
     8701 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
     8761 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
     8821 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
     8881 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
     8941 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
     9001 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
     9061 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
     9121 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
     9181 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
     9241 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
     9301 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
     9361 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
     9421 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
     9481 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
     9541 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
     9601 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
     9661 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
     9721 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
     9781 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
     9841 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
     9901 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
     9961 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
    10021 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
    10081 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
    10141 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
    10201 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
    10261 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
    10321 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
    10381 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
    10441 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
    10501 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
    10561 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
    10621 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
    10681 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
    10741 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
    10801 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
    10861 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
    10921 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
    10981 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
    11041 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
    11101 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
    11161 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
    11221 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
    11281 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
    11341 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
    11401 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
    11461 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
    11521 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
    11581 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
    11641 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
    11701 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
    11761 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
    11821 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
    11881 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
    11941 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
    12001 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
    12061 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
    12121 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    12181 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    12241 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    12301 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    12361 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    12421 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    12481 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    12541 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    12601 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    12661 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    12721 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    12781 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    12841 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    12901 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    12961 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    13021 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    13081 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    13141 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    13201 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    13261 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    13321 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    13381 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    13441 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    13501 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    13561 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    13621 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    13681 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    13741 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    13801 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    13861 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    13921 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    13981 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    14041 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    14101 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    14161 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    14221 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    14281 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    14341 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    14401 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    14461 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    14521 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    14581 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    14641 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    14701 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    14761 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    14821 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    14881 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    14941 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    15001 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    15061 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    15121 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    15181 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    15241 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    15301 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    15361 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    15421 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    15481 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
    15541 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    15601 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    15661 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    15721 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    15781 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    15841 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    15901 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
    15961 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    16021 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    16081 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    16141 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    16201 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    16261 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    16321 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    16381 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    16441 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    16501 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    16561 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    16621 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    16681 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    16741 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    16801 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    16861 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    16921 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    16981 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    17041 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    17101 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    17161 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    17221 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    17281 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    17341 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    17401 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    17461 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    17521 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    17581 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    17641 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    17701 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    17761 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    17821 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    17881 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    17941 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    18001 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    18061 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    18121 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    18181 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    18241 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    18301 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    18361 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    18421 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    18481 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    18541 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    18601 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    18661 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    18721 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    18781 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    18841 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    18901 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    18961 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    19021 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    19081 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    19141 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    19201 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    19261 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    19321 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    19381 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    19441 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    19501 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    19561 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    19621 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    19681 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    19741 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    19801 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    19861 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    19921 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    19981 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    20041 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    20101 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    20161 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    20221 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    20281 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    20341 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    20401 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    20461 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    20521 attttcgact actaataaat gaaagcgcta at
//
LOCUS       DACDBG010000045        19997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDBG010000045 DACDBG010000000
VERSION     DACDBG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.045"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IVZ90_001672"
     CDS             complement(<1..626)
                     /locus_tag="IVZ90_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8262718.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IVZ90_001673"
     CDS             899..3370
                     /locus_tag="IVZ90_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8262719.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IVZ90_001674"
     CDS             complement(3473..5209)
                     /locus_tag="IVZ90_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8262720.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IVZ90_001675"
     CDS             complement(5206..5910)
                     /locus_tag="IVZ90_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262721.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IVZ90_001676"
     CDS             6041..6544
                     /locus_tag="IVZ90_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8262722.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IVZ90_001677"
     CDS             6504..6842
                     /locus_tag="IVZ90_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8262723.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IVZ90_001678"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IVZ90_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8262724.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IVZ90_001679"
     CDS             8393..8971
                     /locus_tag="IVZ90_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8262725.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IVZ90_001680"
     CDS             8975..9496
                     /locus_tag="IVZ90_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8262726.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IVZ90_001681"
     CDS             complement(9575..10129)
                     /locus_tag="IVZ90_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8262727.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="IVZ90_001682"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="IVZ90_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8262728.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="IVZ90_001683"
     CDS             10669..10920
                     /locus_tag="IVZ90_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8262729.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="IVZ90_001684"
     CDS             11045..11836
                     /locus_tag="IVZ90_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262730.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="IVZ90_001685"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="IVZ90_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8262731.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="IVZ90_001686"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="IVZ90_001686"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8262732.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="IVZ90_001687"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="IVZ90_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8262733.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="IVZ90_001688"
     CDS             complement(13893..15029)
                     /locus_tag="IVZ90_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8262734.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="IVZ90_001689"
     CDS             complement(15145..16059)
                     /locus_tag="IVZ90_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8262735.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="IVZ90_001690"
     CDS             complement(16150..16578)
                     /locus_tag="IVZ90_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8262736.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="IVZ90_001691"
     CDS             complement(16583..16957)
                     /locus_tag="IVZ90_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262737.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17243..17428
                     /locus_tag="IVZ90_001692"
     CDS             17243..17428
                     /locus_tag="IVZ90_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8262738.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17440..17688
                     /locus_tag="IVZ90_001693"
     CDS             17440..17688
                     /locus_tag="IVZ90_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262739.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17719..17898
                     /locus_tag="IVZ90_001694"
     CDS             17719..17898
                     /locus_tag="IVZ90_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262740.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17941..18411)
                     /locus_tag="IVZ90_001695"
     CDS             complement(17941..18411)
                     /locus_tag="IVZ90_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262741.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18498..19196
                     /locus_tag="IVZ90_001696"
     CDS             18498..19196
                     /locus_tag="IVZ90_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8262742.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19374..19715
                     /locus_tag="IVZ90_001697"
     CDS             19374..19715
                     /locus_tag="IVZ90_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262743.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19708..>19997
                     /locus_tag="IVZ90_001698"
     CDS             19708..>19997
                     /locus_tag="IVZ90_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8262744.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6561 a         3509 c         3933 g         5994 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    17101 caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat gtctatatac
    17161 taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt acagattatt
    17221 tttttagaaa ggagctattt ttatgtctga gaatttagac ttaaaaattc gagcggagat
    17281 gagaaaaaga agaatgactt tcaaagaact agctgcgctt atcggtattt caggagctta
    17341 tttatcagat attctaaacg gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac
    17401 agtgaaaaaa atattggaca tccgataggg gggtaggcga tgacaaaact aaaaaaacaa
    17461 gattttgtaa aaaaatacaa ttattctcca tctacttatc aacgtcgaat gtcggaacta
    17521 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
    17581 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
    17641 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
    17701 tttgagaggt gaaggttaat gggtaaattc aacagagcat tggtattcag cgcaccgcta
    17761 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
    17821 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
    17881 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
    17941 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
    18001 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
    18061 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
    18121 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
    18181 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
    18241 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
    18301 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
    18361 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
    18421 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
    18481 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
    18541 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
    18601 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
    18661 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
    18721 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
    18781 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
    18841 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
    18901 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
    18961 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
    19021 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
    19081 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
    19141 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
    19201 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
    19261 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
    19321 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
    19381 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
    19441 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
    19501 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
    19561 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
    19621 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
    19681 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
    19741 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
    19801 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
    19861 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
    19921 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
    19981 ggacacacgg aaaccga
//
LOCUS       DACDBG010000046        19474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDBG010000046 DACDBG010000000
VERSION     DACDBG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.046"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..996)
                     /locus_tag="IVZ90_001699"
     CDS             complement(22..996)
                     /locus_tag="IVZ90_001699"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8262745.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="IVZ90_001700"
     CDS             complement(1076..1951)
                     /locus_tag="IVZ90_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8262746.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="IVZ90_001701"
     CDS             2081..3988
                     /locus_tag="IVZ90_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8262747.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="IVZ90_001702"
     CDS             complement(4529..7807)
                     /locus_tag="IVZ90_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8262748.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="IVZ90_001703"
     CDS             complement(8078..8623)
                     /locus_tag="IVZ90_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8262749.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="IVZ90_001704"
     CDS             complement(8635..9327)
                     /locus_tag="IVZ90_001704"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8262750.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="IVZ90_001705"
     CDS             complement(9346..9630)
                     /locus_tag="IVZ90_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262751.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="IVZ90_001706"
     CDS             complement(9643..10206)
                     /locus_tag="IVZ90_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8262752.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="IVZ90_001707"
     CDS             10434..11096
                     /locus_tag="IVZ90_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8262753.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="IVZ90_001708"
     CDS             11220..12416
                     /locus_tag="IVZ90_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8262754.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="IVZ90_001709"
     CDS             complement(12517..13893)
                     /locus_tag="IVZ90_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8262755.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="IVZ90_001710"
     CDS             complement(13930..14796)
                     /locus_tag="IVZ90_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8262756.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="IVZ90_001711"
     CDS             complement(14885..15910)
                     /locus_tag="IVZ90_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8262757.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="IVZ90_001712"
     CDS             complement(15928..16602)
                     /locus_tag="IVZ90_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262758.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="IVZ90_001713"
     CDS             complement(16786..17679)
                     /locus_tag="IVZ90_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8262759.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="IVZ90_001714"
     CDS             complement(17753..19450)
                     /locus_tag="IVZ90_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8262760.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6156 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt ttta
//
LOCUS       DACDBG010000047        19458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDBG010000047 DACDBG010000000
VERSION     DACDBG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.047"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1031)
                     /locus_tag="IVZ90_001715"
     CDS             complement(<1..1031)
                     /locus_tag="IVZ90_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262761.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLY"
     gene            complement(1045..1969)
                     /locus_tag="IVZ90_001716"
                     /pseudo
     CDS             complement(1045..1969)
                     /locus_tag="IVZ90_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2086..3165
                     /locus_tag="IVZ90_001717"
     CDS             2086..3165
                     /locus_tag="IVZ90_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8262762.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            3197..3790
                     /locus_tag="IVZ90_001718"
     CDS             3197..3790
                     /locus_tag="IVZ90_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8262763.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3900..4127)
                     /locus_tag="IVZ90_001719"
     CDS             complement(3900..4127)
                     /locus_tag="IVZ90_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8262764.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(4230..6818)
                     /locus_tag="IVZ90_001720"
     CDS             complement(4230..6818)
                     /locus_tag="IVZ90_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8262765.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6838..7497)
                     /locus_tag="IVZ90_001721"
     CDS             complement(6838..7497)
                     /locus_tag="IVZ90_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8262766.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7546..8049)
                     /gene="trmL"
                     /locus_tag="IVZ90_001722"
     CDS             complement(7546..8049)
                     /gene="trmL"
                     /locus_tag="IVZ90_001722"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8262767.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(8270..9136)
                     /locus_tag="IVZ90_001723"
     CDS             complement(8270..9136)
                     /locus_tag="IVZ90_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8262768.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            9245..9886
                     /locus_tag="IVZ90_001724"
     CDS             9245..9886
                     /locus_tag="IVZ90_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8262769.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9948..11309)
                     /gene="mgtE"
                     /locus_tag="IVZ90_001725"
     CDS             complement(9948..11309)
                     /gene="mgtE"
                     /locus_tag="IVZ90_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8262770.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(11327..12226)
                     /locus_tag="IVZ90_001726"
     CDS             complement(11327..12226)
                     /locus_tag="IVZ90_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8262771.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(12228..13025)
                     /locus_tag="IVZ90_001727"
     CDS             complement(12228..13025)
                     /locus_tag="IVZ90_001727"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8262772.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13003..13707)
                     /locus_tag="IVZ90_001728"
     CDS             complement(13003..13707)
                     /locus_tag="IVZ90_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8262773.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13838..14413
                     /locus_tag="IVZ90_001729"
     CDS             13838..14413
                     /locus_tag="IVZ90_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8262774.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14531..15208
                     /locus_tag="IVZ90_001730"
     CDS             14531..15208
                     /locus_tag="IVZ90_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8262775.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(15287..17095)
                     /gene="pepF"
                     /locus_tag="IVZ90_001731"
     CDS             complement(15287..17095)
                     /gene="pepF"
                     /locus_tag="IVZ90_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8262776.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(17271..18269)
                     /locus_tag="IVZ90_001732"
     CDS             complement(17271..18269)
                     /locus_tag="IVZ90_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8262777.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18495..19148)
                     /locus_tag="IVZ90_001733"
     CDS             complement(18495..19148)
                     /locus_tag="IVZ90_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8262778.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5651 a         4216 c         3365 g         6226 t
ORIGIN      
        1 aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg accaacaatc
       61 ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc agcaaaaaag
      121 ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt catttttggt
      181 atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag agcggtaaca
      241 atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg aaagacagct
      301 aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa
      361 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
      421 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
      481 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
      541 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
      601 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
      661 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
      721 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
      781 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
      841 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc gagattgcca
      901 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
      961 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
     1021 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
     1081 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
     1141 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
     1201 acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg ttttctggga
     1261 gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt agagattctt
     1321 tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg caaagaattt
     1381 ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga ttcagctcct
     1441 tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc ggctcatcta
     1501 aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc
     1561 caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact aattgaatca
     1621 cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg caaaaataat
     1681 cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga acgaccccaa
     1741 tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat ttaatggttc
     1801 ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc ccactgccgt
     1861 ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa tgattcaatg
     1921 ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc atgaaaaact
     1981 ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa ctagtgacga
     2041 ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca taaatctcgg
     2101 ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt aagtcaagga
     2161 ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg tgtcagttat
     2221 ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt atgtgccttt
     2281 ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc
     2341 caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg taatcatccc
     2401 cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt acgaaaaaaa
     2461 gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac tcattgggaa
     2521 aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga aacacaatta
     2581 gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga caatgtcggt
     2641 catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca
     2701 aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat ggatcggatt
     2761 cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc cttagagttt
     2821 ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga aggattgaat
     2881 gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa
     2941 catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt ttatcgcttg
     3001 caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat tggtttgaaa
     3061 acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca agaaaaccag
     3121 caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca gtagtcatta
     3181 ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat
     3241 ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg aaacccatcg
     3301 tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg atgctgccaa
     3361 agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat tcaaagatga
     3421 agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca aacagaactt
     3481 gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat ttggtaatga
     3541 aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata gtcatgctta
     3601 tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa
     3661 taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt acatcagcca
     3721 attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt tttatcagca
     3781 tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg catcattcag
     3841 gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat
     3901 caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg cggaaaaata
     3961 ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg ttctacatca
     4021 ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa gtcattgcca
     4081 gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa gtaacgcccc
     4141 ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc taaccaacga
     4201 tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat aaaatcgtag
     4261 ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat cacttctgct
     4321 gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc ttctccaaca
     4381 aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt ttcattcgtc
     4441 ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt tgtatagttc
     4501 ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag tttggtccgg
     4561 agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt catccggctt
     4621 gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa aatcaacagt
     4681 tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg ctgatattga
     4741 tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg gattgagcaa
     4801 caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc atttgcatca
     4861 aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat gatacccgtg
     4921 atttctccca tatctccatt gaatacattc aattcgggag tattgactaa ttggaggact
     4981 ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc aggtttccct
     5041 ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg tccccgatac
     5101 attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc tttttccaca
     5161 attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga acgatctttt
     5221 tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc aagaggaata
     5281 atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg aatttgttcg
     5341 atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg atctttgtct
     5401 cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt agccagccat
     5461 gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc tttcgtactg
     5521 actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt actggcaggc
     5581 agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc aagtagaata
     5641 ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa
     5701 agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc tgttaaaata
     5761 aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc atactgtatg
     5821 ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg ataatggatt
     5881 tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc agaaaagtac
     5941 aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc aacaagttgg
     6001 ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt ttctaaaata
     6061 cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca
     6121 tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc tgctgcaata
     6181 ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat gtcttcgact
     6241 agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc ctggtagata
     6301 ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac ttgatccatc
     6361 ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt caaaccggag
     6421 atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc tcccagcaat
     6481 tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct ggaaagatac
     6541 gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag tttaagctgc
     6601 cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc attttcttga
     6661 atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc tgtggtttca
     6721 ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca
     6781 actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct ccttcttcta
     6841 tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc atactttcag
     6901 ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac tactattgaa
     6961 caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt gaatggctgc
     7021 agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt gggcaaccgc
     7081 atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg
     7141 agaaggatca tacagatcta aatgattacg caaattcggc agattatccg gatatttctg
     7201 gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc ttagcttatc
     7261 tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg ctgtatcacg
     7321 tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta cctttttccc
     7381 aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat tgctcccttg
     7441 aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata gttccatacg
     7501 cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat ccagcttgtc
     7561 attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa gtgcttcata
     7621 cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat cattcatagg
     7681 gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac cggttgtttc
     7741 ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac tatatacttt
     7801 atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta aaaaagcttc
     7861 taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg cccgttttag
     7921 atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag tattcgtggc
     7981 tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa ataaaacgat
     8041 atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct gacaatttga
     8101 tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt
     8161 gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca
     8221 acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat taaaaactgc
     8281 tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta atcttcttca
     8341 aaggattctt atataagtct gctttgattc gctcttctac ctgccattct aaaggtgaca
     8401 tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt acacgctgac
     8461 gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg gaataactct
     8521 gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa ttttcttgtg
     8581 gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct tggtaatgag
     8641 aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc aaattggtca
     8701 aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag actccttctt
     8761 ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt tcattcaaga
     8821 agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa
     8881 tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt ggaacgaaca
     8941 ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg
     9001 aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt tttacttctt
     9061 cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg cgtcctcggt
     9121 caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta gatgctcact
     9181 ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata aaggagaaga
     9241 ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag caattgctag
     9301 aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac
     9361 tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg taatgacgat
     9421 tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa tcatgcatac
     9481 tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt gtcttactcc
     9541 ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag aagggttgca
     9601 gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg aagcaatcga
     9661 ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac
     9721 tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg gccgcttaat
     9781 cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta aagaattgaa
     9841 cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa
     9901 aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta cataaaatag
     9961 cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc gctcaaagtc
    10021 gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat tagcatcggg
    10081 attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat ccctatgaca
    10141 aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt gatccccgta
    10201 acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac aaatccatga
    10261 tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg ggttccagca
    10321 ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc ttcacttacc
    10381 aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc
    10441 agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc ttccacattg
    10501 accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc gatgatatca
    10561 tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag agctaagaag
    10621 tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg aactgaaagc
    10681 actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc
    10741 agagaaataa cccctactag ctgaccttct tgattaacga catagatgta atagattgtt
    10801 tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt ttgattggct
    10861 acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc gtaatggaga
    10921 agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt agcaatttgc
    10981 tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc agaaagcata
    11041 tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc gttgtcttct
    11101 tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata gatcagctgc
    11161 ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg catctctaaa
    11221 aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa ctgggcaaat
    11281 ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc
    11341 gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc aactcttctg
    11401 taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt attcccagat
    11461 ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa
    11521 ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg atttctgtta
    11581 gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag accattcgct
    11641 tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt gccgataaat
    11701 ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt tcacgaagtt
    11761 gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat
    11821 cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt tttttataat
    11881 aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga atagacgaaa
    11941 cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata tcgatcggtg
    12001 tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg gttaccttat
    12061 catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg atttgaccgc
    12121 cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt aaaaaatatt
    12181 ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca atcctcacta
    12241 ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc aaaatggatc
    12301 cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac attcaattga
    12361 tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca ttcatcatgg
    12421 gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga agcaatctct
    12481 gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact tttattatat
    12541 gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact ttcaaacagc
    12601 tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat cgttgattca
    12661 ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg aacatccagc
    12721 aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag ttcttccagc
    12781 tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc
    12841 acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg
    12901 actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt
    12961 gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac gatcgctact
    13021 ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat agtagacatc
    13081 atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt cttcacggat
    13141 actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa gtcgttcact
    13201 catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa tcgttgccca
    13261 gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct caccgctgat
    13321 cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg cttttttatt
    13381 tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga tgatctcaac
    13441 aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa tatcagacat
    13501 ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta caatactatc
    13561 gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt gttcacggaa
    13621 ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt catatggtgc
    13681 taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa cattcattgt
    13741 ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg accaaaaaaa
    13801 gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga
    13861 atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact atcaattaac
    13921 agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg aacagctgaa
    13981 gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat atacattaaa
    14041 aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag aagaagcaga
    14101 agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa aactttctga
    14161 tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa aacgggctga
    14221 attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca atctacacga
    14281 ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt ttataaattt
    14341 tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac gtatgcttga
    14401 agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt tttacttttt
    14461 tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat ctattttggg
    14521 ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat actgtttcga
    14581 aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat caaaagaaaa
    14641 gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg tcatgcaaca
    14701 aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa tgacttattc
    14761 tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt
    14821 gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag aacttgctga
    14881 atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc gtcagtctga
    14941 cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg gcatcacaaa
    15001 acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg ttttagtaga
    15061 agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa cagataactc
    15121 atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc attcaagaac
    15181 tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac aagagagccg
    15241 acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat aaacgatcga
    15301 tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct tcaatatatg
    15361 cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa tcgctgttac
    15421 ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt agtattttct
    15481 tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag taattataat
    15541 aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg actgctggac
    15601 cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta gtcaatggca
    15661 cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt gtctggcgga
    15721 aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt acacgagggt
    15781 ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc gaagcaattt
    15841 ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga gtgaaataac
    15901 tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc gtatcatgcc
    15961 aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca ccgcttcgtt
    16021 tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct acgatggcca
    16081 tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct tcttcgtacg
    16141 taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg tgcagttttt
    16201 ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc aaatgcttgt
    16261 ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg ctcaaagacg
    16321 cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct tttatatgtg
    16381 tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac aatcccttaa
    16441 atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac acgccatgag
    16501 ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga tctgcattat
    16561 tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca gcaagaagag
    16621 attcctgctc agcagataag acatgggctc gattatctac tatttgctgg atataatggc
    16681 gatagacttc caattttggt tcttctttga aatattgcca aatctggtca tctgacaatt
    16741 gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac agactgcttg
    16801 ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt ttaaggtgcg
    16861 catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca atcgcattta
    16921 aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt tttaactctt
    16981 ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc ttggtcaagt
    17041 cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact tccatgaaat
    17101 ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta acatccacat
    17161 gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt ttagcataac
    17221 taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta ctattttttc
    17281 aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc ttcatcaatc
    17341 ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga gttgaaatca
    17401 ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa aacctgttct
    17461 tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg caaaagatgt
    17521 ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt gtgaataagt
    17581 tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt catttggttc
    17641 attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa agtccagcag
    17701 ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata caaataaagc
    17761 ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt atagtagcag
    17821 caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc aagtatccac
    17881 catacttggt aactgttatt caaataattt tgggttcttt caataaatct ctggtgagac
    17941 agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg cctttgccgc
    18001 aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag ccaatccatg
    18061 aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac cgaacagtta
    18121 tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa aatcactctt
    18181 tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc aaaacagcac
    18241 ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt tacgtctaag
    18301 aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag taataagaac
    18361 agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac cgcagatgca
    18421 agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc acagtctctt
    18481 actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt acgcgccatc
    18541 agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc agcaaactcc
    18601 aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat ccagaagtga
    18661 acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga ctctagttcg
    18721 acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt tgcttgtctt
    18781 tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc aataatctgc
    18841 tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga ttgagaaatt
    18901 tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt tggcaaaact
    18961 tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc ttccaaaata
    19021 ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa tgaaacgccc
    19081 cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt aatatgttcc
    19141 atttccattt tgctacacct cactttgcca caatgtttaa tatagattca ttgtagcgaa
    19201 atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata aaaccagcgg
    19261 atttttcaac ctttcaagct atcttattta actataacat ttttggcaga cgaatactat
    19321 atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc atcttctact
    19381 acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact tggaaaaact
    19441 atctttgtaa tagtcttt
//
LOCUS       DACDBG010000048        19404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDBG010000048 DACDBG010000000
VERSION     DACDBG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.048"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..1302)
                     /locus_tag="IVZ90_001734"
     CDS             complement(118..1302)
                     /locus_tag="IVZ90_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8262779.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1327..2334)
                     /locus_tag="IVZ90_001735"
     CDS             complement(1327..2334)
                     /locus_tag="IVZ90_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8262780.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2506..2865)
                     /locus_tag="IVZ90_001736"
     CDS             complement(2506..2865)
                     /locus_tag="IVZ90_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262781.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2837..3208)
                     /locus_tag="IVZ90_001737"
     CDS             complement(2837..3208)
                     /locus_tag="IVZ90_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8262782.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3225..3527)
                     /locus_tag="IVZ90_001738"
     CDS             complement(3225..3527)
                     /locus_tag="IVZ90_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262783.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3544..4014)
                     /locus_tag="IVZ90_001739"
     CDS             complement(3544..4014)
                     /locus_tag="IVZ90_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8262784.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3989..4267)
                     /locus_tag="IVZ90_001740"
     CDS             complement(3989..4267)
                     /locus_tag="IVZ90_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8262785.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4264..5313)
                     /locus_tag="IVZ90_001741"
     CDS             complement(4264..5313)
                     /locus_tag="IVZ90_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8262786.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5270..6199)
                     /gene="cpaF"
                     /locus_tag="IVZ90_001742"
     CDS             complement(5270..6199)
                     /gene="cpaF"
                     /locus_tag="IVZ90_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8262787.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6331..8085
                     /gene="ade"
                     /locus_tag="IVZ90_001743"
     CDS             6331..8085
                     /gene="ade"
                     /locus_tag="IVZ90_001743"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8262788.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8183..8845)
                     /gene="trmB"
                     /locus_tag="IVZ90_001744"
     CDS             complement(8183..8845)
                     /gene="trmB"
                     /locus_tag="IVZ90_001744"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8262789.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8915..9694)
                     /locus_tag="IVZ90_001745"
     CDS             complement(8915..9694)
                     /locus_tag="IVZ90_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8262790.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9901..11112)
                     /locus_tag="IVZ90_001746"
     CDS             complement(9901..11112)
                     /locus_tag="IVZ90_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262791.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11105..11842)
                     /locus_tag="IVZ90_001747"
     CDS             complement(11105..11842)
                     /locus_tag="IVZ90_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262792.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11980..12414
                     /locus_tag="IVZ90_001748"
     CDS             11980..12414
                     /locus_tag="IVZ90_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8262793.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12407..12751
                     /locus_tag="IVZ90_001749"
     CDS             12407..12751
                     /locus_tag="IVZ90_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8262794.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12931..13941
                     /locus_tag="IVZ90_001750"
     CDS             12931..13941
                     /locus_tag="IVZ90_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8262795.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(14010..14954)
                     /locus_tag="IVZ90_001751"
     CDS             complement(14010..14954)
                     /locus_tag="IVZ90_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8262796.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14938..17658)
                     /locus_tag="IVZ90_001752"
     CDS             complement(14938..17658)
                     /locus_tag="IVZ90_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8262797.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17655..18875)
                     /locus_tag="IVZ90_001753"
     CDS             complement(17655..18875)
                     /locus_tag="IVZ90_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8262798.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19156..>19404)
                     /locus_tag="IVZ90_001754"
     CDS             complement(19156..>19404)
                     /locus_tag="IVZ90_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8262799.1"
                     /translation="EIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESAD
                     ISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         5894 a         3939 c         3232 g         6339 t
ORIGIN      
        1 aaaaaagaag ggaaattgaa gttaaattac cattttgcgg tcttctccgc aaaaaaaaca
       61 catgcaagaa ttccctcttg catgcgtttc atcttttatc ctaacgaatg ctttggctta
      121 gccgcgcaga cgctccacat cacgggcaat cattagttcc tcatctgttg ggatcactaa
      181 tactcttact tttgattctt ttgtagagat atctacttct tcaccacgca attcattcaa
      241 cgctggatcg atttcacaac caaaccaaga catacctttg atgatttcac tacgtacatg
      301 tccgtcattc tcgccgatac cagcagtaaa gacaattgca tcaacaccat tcatcgttgt
      361 cacataaccg ccgatgtatt ttcggatgcg gtccacgaaa atgtcataag ctaaacgaat
      421 atgttctttt tcatagttct tttcaagatc acgcatatca ctagacaatc ctgttaggcc
      481 tagcagacca gatttcttgt tcaaaacatt gatcatttca tcaatatcaa tctctaattt
      541 ttccattaaa tatgggagaa cagctggatc gatatcacct gaacgtgtac ccattgtcac
      601 cccagctaat ggtgtgaatc ccatagaagt atccatggat ttacctccat cgactgctgt
      661 gatcgataca ccatttccta agtggcaagt gatgattttt agttcttcga tcggtttgtt
      721 caataatttt gccgcacgtt ccgacacata acgatgactt gtgccatgga agccatattt
      781 tcttactttg aaatcttcat aataagacac cggtaagcta tataaataat tttgctttgg
      841 cattgttgag tggaaagatg tatcgaatac agccacactc aaaatgtcag gtaaaatctt
      901 tttaaacgct ttgatcccca ttaagtttgc aggattatgc aaaggagcaa attctgctaa
      961 ttcagcaatg cggtccatta ctttctcatc gatcactact gaatccccat aagtttctcc
     1021 tccgtgaaca acacggtgcc ctactccggt gatttcatca taagagctta aaatttgcag
     1081 ttcaatcaac tgatctaaca gcatttttac tgctacttcg tggttttcga tatccatgat
     1141 ttgttcgaat ttttgattat cgccgtactt gatggtaaaa atagaatctt gtaaaccgat
     1201 acgttcgacg atccctttcg caactacggt ttcttctggc atttgatata attgccattt
     1261 caaacttgaa ctcccagcat tgattgcaat tgttttagac atatgactct ccttctcgtt
     1321 gaatgattac agagcagatg atttccagtt tttaaactcg ttgaaaaatt ccgtcacctt
     1381 ctctggttct tttaatgaag caagtttcac aagcaagact tcttttactt gttgtgcttg
     1441 tggtcctttt ttctgtaaga tcaaaatact tttttgcgat tgtttattac ggaacaattc
     1501 atctggcaat tgaatcattc cttgaagata accttcttct ttaaaccatt ttttgatttc
     1561 ttcactttga tccgtttcta aaaatccact cggcatcaaa aacaaaccaa aaccatccgg
     1621 cttcacatat ttcattgatt gctctagcaa taaatgatgt gcataactat ggccttcttc
     1681 ggtacttgtt aaaaactctt ttgctttttc gtcattcgga taataaccga ttggcaaatc
     1741 gctgattgca aaatcgacag gatctattaa taattcctgc aacccgtctt gatgaaagta
     1801 ttgaacattt gcttgtgtca aatcacttgt agaagctgcg acagccaaca atgtatcatc
     1861 aatatccaca ccaattcctt ctgtttggta ccccgcattc gataggttta aaagaactgt
     1921 taataacaag ttgcccattc ctgccgcaat atccagaatc ttaaccggtg cttttttatt
     1981 tgtgtatagt tgttcaataa gaaaaacaaa taaaaaaccg atactatcag gagtgagttg
     2041 atggttcgcc tgcaaatgtt ctgtctggct gcccttcaat aaaagtaatt gagacagtct
     2101 gcgccattct tctggctcga aagaaagttt cttcaactct tcataaagag ctgtgatccg
     2161 ttgcgttgtc tcttttgtcg gcactccatc caccacacgt acttgataat catctattaa
     2221 attttctgca ttttcgacat aggcttctaa aaaggaagtt cccaatgcat tctgtaaaag
     2281 ctgaatagct tccaggtttt gattaaaccc ttgttccatt ttttctggta acatggtgca
     2341 cctcatttct agatatatta taacaatttt ttcacctgtt taattctatc gaacaattga
     2401 caaaatttca atcgttttca ctgtttttta tttgtgaaac ggcaaacatt tttttattaa
     2461 aaacagtgtt ataacagaaa agagggcgtt ttcacgaaca ttccatcatt ctgtactagt
     2521 atctgtatca ttttttactt cttctgtgaa ccgaaagata aacggtgaga cttccacctc
     2581 taagaatagg gactgttgta cccactgata tgttacagat ccagttgtgt agtaaaaaac
     2641 tccttccgaa ctttcggcaa gcgtagactg ttccgataaa gccatctgct tcattatttt
     2701 tgctatataa taatttttga ttcgtatatt aagatccatt gacaaacggt agctttctaa
     2761 tgctgccatc aataaaaacg tacataaaag aaataaaagc aagacagaaa ataaaataga
     2821 gcccttataa cattttctac tgatcgtttt ttgtccatat ggcatatcca tcttctccat
     2881 tttcaaagtg tacataaaaa gaaattgtgt gttctttttc caataattga agctcagtga
     2941 tacttgttaa cataggttga tgtccgccgg aagttcttct tttacgtatc attgcctgat
     3001 atctttcgat gatatactgt ttatcctcga tcgtgtagtg cagttgctga tttttcacag
     3061 agacattgtg actgctcttg agttcttctt ctaattgtgc taaaaatatt agccactctt
     3121 tttgatcttt acgctgcaaa aattcactta cttggatggt ttgctgaatc aaaagattga
     3181 atgtgagcaa tagactgctc aatatcatca gtccaattag acattcaatc agcgtaaatg
     3241 ctggaattac ttgttttttc aatactgatc gcttctttct ccatattgac aaacactttt
     3301 tcgattactc cattattatc gacaatttgg atatcgtatg ggatcatttc ttcccttcgc
     3361 tctggtcttt tgtgatacat ccgctgcgtc ttgatatctt cataaatcat tcgaataaat
     3421 agcagttcct ccccactttt cttttctgcc tgaagcaaaa acagctggcc aaagctcact
     3481 aagtagacac agacagaagc gatcataagc gctgtaaggg actccaacaa aacaaatcct
     3541 gttttattca attctttttg tatagcgacc actccctatc tggaattgat acgtaatcgt
     3601 ccgctctttt tcttcccaat aaaattgata agcttttagt gaactttcat tcccggttct
     3661 agcagcaaaa gtaatagaag catggcgttt caaggtaatc tcatcaggaa tatctaatac
     3721 ttcccaattt attttatctg gattcggaac atgaaaaatg atttgattct cttcactatt
     3781 ccaataaaag cctgtttgga tctgattcac aatagcgatt ttttgcgtag aatagattcg
     3841 tttttcaaat tggccgaaaa attgatagac tgccaattca ttcttccatg cagaaaatgc
     3901 gagaactgga aaagaaagga tcgttgtaag taataaaatc agtaagagtg attcaaacaa
     3961 cgtatatcct gatgctaaaa ccttaccttc aaggccgctg atagatttct gcatgttctc
     4021 gtttgatata gccttcattc aacaattcct ccacagtagg aactcttcca ttattttgaa
     4081 gcgcatagag ctctttttgt gtgtctacca actggatgat cgcttcacga ctttcttggt
     4141 ctacatgatt cctataacgc gacaaattag ggacgaacaa caacactaat acactgatga
     4201 tcagtaagac gattagcatt tcgatcaatg taaaaccgct ataataagat atttttctct
     4261 ttttcatggc aaaacctcct ctatgcttcc gtaaacaggt aataaaacag cggcataaac
     4321 taataaaatc aaagccgcta cgcccaaaaa aatgattggc tgaataaaat gcatccagtg
     4381 atgaagacgt ttgaaaagct gttttcttgt ccattcgctg taaaaaatca gttctttacc
     4441 taaacttccc tttgcctctc cctgtaaaac aattttactg aattcttctg atagaaaacg
     4501 ataattctgg aactgctcag ccaatgacat acctgcctcc aatccttgaa tcaacttaga
     4561 ggcaagatgc tgaatcagac tttcttgttt cgtttccttt aagcagacta tgatttgcct
     4621 taattcgaaa ccttcatgaa aaagctttcc aatctctagt gacaaataag ccgattgata
     4681 caaggaatat atcggtccaa tcaataattg tctgcttatc cattcactct gatcaacagg
     4741 agacaacttc atcagacgat accttactag taaagccaat agagtaaccg ctaggatgaa
     4801 tagtagaaca taccaatgaa agacttgtat cagctgaata ccccaatgct cttgcatgac
     4861 cattcctgaa ccaataagct gaggcaaaac gaacaaccgc atactaaaca agatacctag
     4921 caaaaaggtg agcaatagca tcggatatgc aagcagcttt ttcaaatcat tgcgaaatcc
     4981 ttcaacaagc cgaaactgtt tggcaatttc caacaaagtt gtcggcagat tcccatgacg
     5041 ttcagctaac tcgatctgta ccagttgctc tgctccatat cctatttgcg aaagtacctc
     5101 actaaacgct tgcccttcct ttagctgatg ttggatattt gccaaaataa caggtggaaa
     5161 cgaacggatc gtgagtaata aataaaggga ttcttgcaga ctaaaaccag ataaaagcag
     5221 ttctcccatg gtttgtgcaa actggttttg ctggcttctt gttaatcctt tagaatagtt
     5281 ctgactgcca aatattttcc tgagcttctt cataactttc ctcccatgat ttttttactg
     5341 attcttcctc cataaattga taactccata aaacagaagg atggtgattg gcatcttcca
     5401 aaagttcttg ataaatgaca ccagctaaac attcagatag ctcttctttt ttctttgtca
     5461 gttcatggat tctgaccaat gtaccttcaa gattgcgtgc atgtacggtt gcaaaaaccc
     5521 ggtgccctgt tagtgctgct cttaccgcag catgtgcagt tgattcatct cttatctcgc
     5581 caatgattag caagtctgga cgatgccgta atgcgagttt caaaagtgta tcataagtct
     5641 gtgctatttt cggattgatc tgtagctgca gaaaaaacgg ttcttctatc tctacaggat
     5701 cttcgatagt aatcacttgc aatttttctt ccaaagccaa ccgatacatt agagaagtct
     5761 tgcctgatcc gactgtcccg ctgaaaaggt ataatccacg tctgacggtt ttctgtaaaa
     5821 tagttgcaaa atctcgagaa ctaaagcaac gatggggtgt ttcaaaagta ccgtataata
     5881 gccgaatcac taaactctct cgttgaaggt aatctcctac agaagacaac cgcagacgaa
     5941 cttcctgttt ttctaaagaa taagtaaatg cacctaactg agcttttctt ttttctccga
     6001 tatccatctg tcccaaatac ttaaatcttg agatcagttg ctgtccatct gccgaagaga
     6061 ctttcttcca gaatgttcgt ttttttccat agcgaaaata gatgtaaaat acttctgaag
     6121 ctggcaatat atatacgtct tgggcatgtt tggcaaaact ttcacttatc atttgatccg
     6181 ataatcgttt tatttccatt attctcccct ccacaaaaaa catacgtctt tttgatcttt
     6241 ttgtttcaaa taaaatgaat ttacaaaaaa atattggcaa aagccgaatt tatgttatac
     6301 ttataaaaat ttatcttggg aggcactcca ttggaaacaa cattaaggaa atatatcgaa
     6361 gtagcaagta aaaaaaagcc tgctgaccta gtgatcaagc atgctaagat cgtcaacgtc
     6421 tttacgaaag aaatcatgga aaaagatgta gcaatctgcg aaggaatgat cgtaggaatc
     6481 ggtgaatacg aaggaaaaag cgtacataat gctaatgggc aatatctagt gccaggattc
     6541 atcgatggcc atgtgcatat cgaaagctca cttttatcac caagcgaatt ttcaaaagta
     6601 tccttgcttc atggagttac tactgtagtg acagatcccc atgaaatagg aaacattgct
     6661 gggagtacag gtcttgagtt tatgatagaa gatgcaagac aaacactcat gaatatcttt
     6721 tttatgctac cttcttgtgt acctgtaacc ccttttgaga ccaatggtgc aaatttaaat
     6781 gcaaaaaaat tagccccttt tttacaaaaa cccgaagttt taggcttagc tgaagtcatg
     6841 aactatcaag cagtagcaaa taatgaaaca gacatcctag aaaagatcca attgatgcac
     6901 cagcataaga aaaaaattga cgggcatgca gctggtatcg gcatggaaga gttaaatgtt
     6961 tatcctgcag caggtattcg gacggatcat gaagctacaa ctgcaaaaga agctaaagaa
     7021 cgtttggatc ttggtatgta tttaatggtt cgagaaggga ccgttgcaaa agatctggca
     7081 tcgctactac cggcgatcac acctgaaaat tcaagaagat gcttctttgt caccgatgat
     7141 aaactgatca atgatttagt aaaagaagga tcaatcgatc atatcgtccg tcaagctatt
     7201 gcacttggaa tcgatccatt acaagccatt cagatggcaa cattgaatgc agcagaatgt
     7261 tttgatttga aaacactagg tgcgattgcc cctgggtatc aagctgattt cttcttaaca
     7321 gacgatctta ccaagctgcc aattcatgaa gttttctata agggaacatt agttgttcaa
     7381 tctgggcaac tccaagacca tttatttgct gatcattcaa attcatatac gtgttgtctg
     7441 ccaaaactaa acgctcaacc attaaatcca cattcgttag agctgcttct ttcttcttca
     7501 caagcccatg tgatcgaaat tatcccgaat agtctagtga ctaaagattt ggtagaaaaa
     7561 gtggaccgac aaggcgaacg attcattcct tcaatcgaaa aagaccaatt aaagattgcg
     7621 gtgatcgagc gtcaccatca aacgggaaac attggattag gcatagtgaa aggatttcag
     7681 ctgaaacaag gagcgatagc gacaacgatt gcccatgact cgcataatct agttgtcgtt
     7741 gggacaaacg acgaggatat gctgtatgct gctaatcttt tgattcaaaa aggtggcggg
     7801 atgaccgttg tcaacaaaca gcaggaaata gcttgtgttt ctcttcctat aggcggaatc
     7861 atgacacagg aacctttttc gatcgtctat gaacagttga atgaattaat aactaaagca
     7921 catcaactag gagcagctaa aacctttgat ccttttttga cactttcttt cttaacatta
     7981 tctgtcatac ccgaattgaa gatcaccgac aaaggattat tttcattttc tcgcttccaa
     8041 ttgattccac cttgtggaaa aaccgatgaa ataaatacat tttaaatcaa ataaatccca
     8101 gaaaatacat tacatctgta atgttcattt tctgagattt atttatggat attaatgttt
     8161 ttatgaaagc agaaagttag tttcacagac ttttcttttt ggtcttatct tgaaattgag
     8221 cttctactcg ataaatacgt tgaccttttg aagaaaattt ttcttcatac tccgtcataa
     8281 tattgccttc aaattgactt tgatgtaaat ctaaccatac ctgtttgagg atcatgccat
     8341 attgagaaaa acttgccaga gaatactcaa acaatccttg attatctgtt ttgaaatgga
     8401 tttctccatt tggacgaaga atctgttcat ctacagcaag aaaagtctta tatgtcaatc
     8461 gccgtttttc atgacgtttt tttggccatg gatcggaaaa attcagatag atttgatcta
     8521 cttcgctgtc agcgaaatac tctgtcaacg ctgatccatc cacatgtaga agtttcaggt
     8581 taggaagcgg ttgttcgatc aacttgtcta gtgcaatcga aacaacactg acctgcattt
     8641 ctactccaat ataattgatt tcaggatggg cttttgccat tccagtgatg aactggcctt
     8701 tccccatacc aatctcaatg tggattggat gatcgttttc aaacaattca ttccattttc
     8761 ccttccacaa tgttggatca ctaattacaa aattcgggtg agccgctaac atttccgctg
     8821 ctcccggacg atttcggaca cgcattattt atctccattc ctttcctcta attaaaaagg
     8881 tctggccaaa tctccgcaga gtttgggcca aacctcagcc actgaaaatt cgttttaatt
     8941 gtaaaatttc ttcgttcatc cgtcgatgat cttcacgttt gcaatatctg gtgatttctt
     9001 ggagcaaatt catggccgca taccaatgaa atttttcaag catttcgtca gtcgggcgta
     9061 tgccataact gatcagccac tgattccagc tagacagtgg cacataattt cctaaaatcg
     9121 ttgcaatatc caccgcaggg tctgcaaaca tgattgaatc ccaatcgact agatataaat
     9181 aatttttaca tactaaccaa ttacgatggt ttacatctcc gtgtactact gataagtttt
     9241 ccttatctat tgacgggatc tgctcacgaa gaaaacgatg gacaatttgc agaaattgat
     9301 ttttctggag ttcttccggt aattcttttt catatttttg cagcatcttc tccggcgtca
     9361 tgacacgacc accgatacgg gtcagcatgg ttttaagaga atttgaatga tgtaaatggt
     9421 acaaaacatc aatcacgtcg tttctttgac caatctcatt aggagtcagc aagcgacctt
     9481 ccagccactc ttgtgctgtc aaagtatcgc cattccccgt tcgttttgtc caaacaagtt
     9541 ttggtgtaat tccttccctt gaaagagcag ccaacatcgg agtcgtgttt cgtttaataa
     9601 agactttgtc ctcatcacgc atgcctatgt atgtttttcc tgtgtcgcct ttgatgggac
     9661 gtaaccgcca ctcttggtct aactgaaact ccatcatgca ttctcccgtc ctcttactct
     9721 ttgttttgca gtacttatta ttctagtcgc ctgaacacta cacgtcaagc acatatacgt
     9781 tctttttcat ttaattgcca ttcaaaaaac cagcttttct caaaaaaaat agaacgaaca
     9841 gcattcttac tgtcaacgct ctatttttca ttctcatttt taccctacac agctttgttt
     9901 tcagtctgac atcttcttca aacgagttgg tacatatagt ttaacaaaga gcatagtaac
     9961 aatcaaataa gccgctagcg gaacccatcg ctgctgccca tctaatacga ctgcagcaat
    10021 caaaccaaaa ataaccgacg cgataattaa tacccatgtg agtagatact gaatcgcctg
    10081 cttcttctga ttcttatgga ttggataaag ctgaacgaga accatatagc ggaactggtt
    10141 cgctaaaggt aacagttgga aaccgatcaa atataaaaat aagaaaccga taatcagact
    10201 aaaataaaag tcgactactc cggcaatcaa taacccacct ataattacta atcggaaata
    10261 aagaccacta aaatcagaac ctcttgccaa tcttcttgca aacaagtaaa gataagtgtt
    10321 ttttgattct cttttaatat gagtcaatat aggatccaaa tagcgtcttc ttttgatctt
    10381 tgccgtaatt tcaggaacat ctgtgaataa attgataaat tgatagatct gatgcaacct
    10441 atgttcttct acatgcacca ttttctccca atccaatcct ttttcttgtt gcttccacaa
    10501 aaaccagtaa tatagacttg cctgtactaa tgcacctgct attcctgccc atattagcca
    10561 ataaaatgat agaaacaaaa agatcagaga agaaaaaaac cagcctaatt tccaataacg
    10621 aataacttgt tgatctagtc gaaacaattc tgctctcttg atttgcagat gactgaattt
    10681 caaactccat aatatgatca gatagacgaa aaacatcgaa aaggattgct ccgtactaac
    10741 caccactaat ggcatggcca atccgcatgc caatatcaaa aagatcaacg gaaaaatgca
    10801 agaatagcga aaagctcgtt ccaaataggt ttgcatttct ttttctttag gaagtaaaaa
    10861 aacaatatct gctgcctctg caaaagtagc catcttaccc acgaataaac agagcatcca
    10921 aaatacagag attatgagcc ctccaattgc aaacggtggc tgcagggttt tcaaccaatt
    10981 agaataatat aatcccacac cgccaatcaa gaaaagacaa actaaaacaa aatgatcgtt
    11041 caatacataa cgcatatatt tcatcatttt cttttgatgt cttgctaaac gtttttgaaa
    11101 aaaactactc atgtgcctgc tcctttgtta aagcaatata gatttcgtcc aaagaagaat
    11161 ccggcagctg aaattctttt ctcaacatct caagagaacc gtctgctcgt atctctccgt
    11221 tatgtaagac cacaaaacga tcacaatatt tttctgcagt agctaaaata tgcgtagaca
    11281 tcaaaatacc ggctccttct tttttcattt ctaccatcaa ttcaagcaat gcattgatag
    11341 ctagtggatc caagcctaaa aatggttcgt cgatgatata taaagaaggc tcgatcaaaa
    11401 aggcgcataa gatcatcact ttctgtttca ttccctttga aaaattagct gggaaccagt
    11461 caagacgatt agataaccga aaagtctcca acaacttttc tgctcttttc attgctatat
    11521 ccataggtat atcgtaggcc atcgcagtga cttcgatatg ttctctcaat gtcaattctt
    11581 catataaaga aggagtctct ggaatatacc caatttgttt gcgatattct tccgtatttt
    11641 tctttaagga tagatgattg atttctatcg ttccttttga agcttccagc agaccgataa
    11701 tgtgcttgat ggtcgtactt ttccctgctc cgttcagtcc aatcaaccct actatctctc
    11761 caggctgtac atcgaaactg atatctttta acacagggag atgtccgtac ccccctgtga
    11821 catgatcgat aattaaactc attctttttc ctccgccatt tatttttatt tattatacca
    11881 cgaagaaaat gacggtattt tgtttttttc cttacaatag ctttaactta tggtaaagtt
    11941 aagctgaagt atttattctt tatagaaagg atgttttata tggaaaactg tattttttgc
    12001 aaaatcatca atcaagaaat cccaagctat aaaatatatg aagacgacaa ggtctatgct
    12061 tttttagaca tctctcaagc aacgaaagga cacacattag ttgtgcctaa acgtcatgta
    12121 gcagatattt ttgaatatga tcccgaactt gcaggagagg ttttttcgcg tgtgcctaag
    12181 attgctcaag cattggaaaa agcttttcca gaaatgaaag gtcttaatat cttgaataac
    12241 aaccgtgaac tagcctatca atctgttttt cattcacata tccatttagt ccctcgctac
    12301 agcaaagagg atgatttttc tattcacttt gtcaatcatc aagatagtta tggatcggaa
    12361 gaactaaaag ctattcaaga aacaatcgta aagcaggtgt cctgcgatga ttaaaaactt
    12421 tttcaaagga ctgttatttg gagctgctgc tggcactgtc ggaggtctgt tattggcgcc
    12481 caggagcggg aatgaaacaa gaaagaaact gatcgaagaa ttggaagaag caacaactct
    12541 gacaaatgat ttaaataaca gcctgaatca ttttaaaagc gcattgatcg aaacaaaaga
    12601 aacagctgaa tctgtgattc ctgctttcca agaagcaatc aaaaaagata tcgaagattt
    12661 taaattccaa gccgaaccac gtatcacaca aatcaatgaa caagtagaaa aactaaaagc
    12721 tgatctgcca gagtatcctg aacaaatata ataatgatta ttttctcaac agatttaact
    12781 atataagtga gaaaagtttg cgtatttcct aggaatgtga tgaaattttg aattttgttt
    12841 gaaactacca cgacaacgtt tttaaaggaa atgggatatg gtatagttaa tatataaaaa
    12901 taataactaa ttacaggagt gcaaatttta atgaaaaaaa aatcaattat tttggcagct
    12961 actagtgcgt tggctgtttt aactctagca gcttgttcag gcgatactaa taaagatatc
    13021 gcaacgatga aaggcggaac gatcactgta tctgatttct atgatgaagc aaaacttgaa
    13081 tcatcgaacc aatccttagt acaacggatg atcatttata aagtattcaa taacaaatat
    13141 ggcgataaag taaccgataa gcaagtggat gcagaatacg acaaacaagc gaaatcatta
    13201 ggtgatactt ttgaatcaca attagaagct gctggatata cgaaagatac ttataaagaa
    13261 tatattcgta acaatttagc ttttgaagct ggcttgaaag ctcacgtaga tattacagat
    13321 gacgatttga aaactgcttg gaaatctttc catccagaag tagaagcaca aatcatcaaa
    13381 ttatcaagtg aagatgaagc aaaagatgtg aaaaaatcag cggatgatgg agatgatttc
    13441 tctaaattag ccaaagacaa atcaactgat acagagacaa aagaagatgg cggtaaagtt
    13501 aaattcgact caacgacaac aactattcct gcagaagtaa aagaagcagc tttcaaattg
    13561 aaagacggtg agatctccga tgttattact acaacaaatc ctacttctta tgcgacagaa
    13621 tactacgttg tcaaaatggt gaaaaaccaa aataaaggca atgacatgga taaatacaaa
    13681 gatcaactaa aagatatcgc tactgaaact aaactaagtg acaatgcatt cacaactaaa
    13741 gtaatcggtg aagaattaaa agatgcaaat gtaaaaatca aagatgatgc ctttgaaaat
    13801 gtgttgagtg cattcacaac aacttcatca agtacgaaag actcaagtga aactacagct
    13861 tcaacaaaga gttctgacac aaaatcaact gattcaacaa aagaatcttc aactgaagaa
    13921 acaactgatt catcaaaata aaagaaaaca aaaaaagcga agacttatgt ctcgcttttt
    13981 ttgttttgaa tgaaaaaaat aaatttttgc tataaatctt ttggtacata gaaacttcgg
    14041 ttgtccatac cgaaaatccg atcggtgtat tttcctggtt ctgtttgacg aatcgaagtc
    14101 aacatcatct gcatcgaagc atcgatattg tcgatttgat gtaatatttc tgcttccatt
    14161 attcttggac gtacaggtga gccatattct aataagccat ggtgtgctaa aaccatatga
    14221 cgtaatacaa gaacatcttc gtcagcttcg ctgattttca acgcaagaca ggctttagtt
    14281 atttcttcgt caatcaatac cagatgaccg atcaaatttc ctgcaagcgt atattctgtc
    14341 gacataggac cggaaagttc aatcaccttt cctaaatcat gcaaaatgat accagcatac
    14401 aacaatgctc gattcagctc aggatattca tcggcgattg atttccctaa gtgaagcatt
    14461 gtcaaagtat gataagctaa tccactggca aatgcatgat ggtttctttt tgctgccgga
    14521 aactcaaaaa attgtttttg atatttatta agtagatagc gaacaatacg attccaatgc
    14581 gcattggtga tctcaaacaa caattgattg atttcttctt ccatttcttc tttcttcaaa
    14641 ggggctcttt ccatgtataa actaggctct cctggctcat ctacccttgt taagcgcata
    14701 tgaagaatct ttacttgagg gtttccttga taaacctctc ttttaccgtt tagaaaaacg
    14761 actgtgccgg ctgtaaattt attgatttcc tcctccgaag catcccagta tttaccatcg
    14821 atagtacctg aagtatcttg gaaagtaaaa gcaataaatt tttttccgtt tttagcgatt
    14881 cttacatctg cacttttgat caggacaaat tgttcaaact gctcatcgat tactaaatca
    14941 cgaatttttt tcattatttg ctccttctaa ttgttttacg atttggttca tctcatggta
    15001 atagcttacc atttctttat cagaagaaaa acaaattatt tgatactttt ctccaaattt
    15061 agctaaaagt tcagctaatt gatactttcg ctgatgatca taatgaagcc agccatcatc
    15121 aatgatgatc gggcatatcg aacggttagc ttctaatgag aggtaagcaa atctcatcgc
    15181 cataatcagc tgatctttcg tacctgtaga taactcatag atgggaaatt ctgtatatcc
    15241 gtctgttaaa gtcagtgtat catttttcag taatatcttc tgataatttc cttttgttaa
    15301 aatacctaaa taatcacctg ctgctcttaa caactggggt aattgtcttt cactcatctc
    15361 tgttgaaaga tcacctaaga atgtcccggc gatttcaagt gctccccatt cctccagtaa
    15421 ttcctgtaat tttgcttttt gccggctagc ttgttgataa agttcgtcca atgtgccgtc
    15481 tctttgcaac tcttctactt gaagttggag tcgttgccga ttttcacttg cttctttttc
    15541 ttcttgctgg agttgggatt gcttttcttt cagttggcgc agtcttacag ataatttctc
    15601 ttttgtcagc tcttcaggaa atagtggagt aagcatttcc gataattcat tgtatctttt
    15661 catctgctgt tcggcttgtt tggctttttg taaaaagaca ggtacttccg aaggataaga
    15721 taagcctgct tgagtcaatt gttcgctgta ttcttgtatc catacttttt gtttcttttt
    15781 caattgattg atttcttgct tcaataaagt attttgctga tagcttctgg caaatttcat
    15841 ctgttccatc tcttgtataa aacgggaaag caataccatt ttttctgaaa ttgtcttatc
    15901 ttgtatcggt aaccattctt taacaaactc aaatttttgg tcgatttgtc gtagtttttc
    15961 ttgtatttcc tgttctcttt tatgtttccg ctcaaatacg ccatataatt tctgataatc
    16021 tatgacttcc tgtccatatt ctttcatgag tttgagcgta tccatctttc ctaagtgccg
    16081 atgatccgca aaacgatata cttcttgctg aagaatctgt ttttcttctc cagttctctt
    16141 ttgtttttct tctgcttcag ctaattgagc taaggctaga tcaagttgtc ctagtttttc
    16201 ttgccacatt ccttttatct cagagaaatc atttttagtt ttaatttttg gttgcctttt
    16261 gtagaagaaa tagccaatca tgcctgcacc aattagtaaa aggatcactt tcaagggcat
    16321 tggtacaaat atacctgcaa tgattattgc tccgccagcc atcagcatat acggtttaga
    16381 tgaagagact ggtgctatct ctgactgatt ttgtaataat gctggatagg tctcttccaa
    16441 gtcattcact tctgcttcaa tcatctcttg ctgttcttta gctatttgga gacgcacaga
    16501 aatctggctt tctagatcag caatccttct ttcttcagcc agaagatgct tccattcttc
    16561 ttcatataaa aattcttccg gtaacggatt tttctgccaa tgccaccttt gttccaaaat
    16621 cttcaattct gaatttgttt ctctagtctc aatttgtaat ctttgccatt cttcgtttaa
    16681 ttgttccgct gtaaattgaa gttgctggag atcttttagt tcctgctcct gctctaaata
    16741 aaaataatat ctggctgact gctgatccat cccgctatgc ttttccagtg ttgcttctaa
    16801 ttcttccaaa cgctcatttg tttgctgata ccgtttggag aaagactcca gatctaaaag
    16861 aagttgctca tcaggaagac ctgtagtgct gttttttttg agagattgcc attcttcata
    16921 taaagaaaaa ttaagttctt gctgtctggc taatgaaaat tgtttgttcg cctcttttaa
    16981 ttgtgccaat aactgctgct gttgcgactc gtattcctgt tcttgttgga tcaacttgcc
    17041 aaaataagct tcctgttctt gtttttgatg aattttttct tgcagctctt tccattcatt
    17101 taatttttga ttgattagta atttttggcc ttttttcttg aataattgtt gtgctttctg
    17161 ataatattct tgccgctttt ggaatacttg agaactgccc gtgatgccta aagaaatcag
    17221 cgcatcatgt agctcttttt ctctcaaatg atctagttcc atcaactgct cttgctggaa
    17281 agtaaatacc tgctggaata actctttcgt caaaggtgcg atcagttttt ctagtaactc
    17341 atcacttccc acttgatccc ccagccacac cttcgatttt ccgcgatttt gctgcttgta
    17401 gcgctcgaca gcaaattcac catatacagg atgtttaagc catattttgc cgccataggc
    17461 tgagccgtct tttggtgtat aatccttttt tttacggcct tttgaaggga aaccaaataa
    17521 tattgcttga ataaattgat aaatcgttga ttttcctgct tcgtttcccc catagatcag
    17581 ctgattgcct gaataaaagt caaaactttt ttgacgccat tttccaaaag cagcaatctc
    17641 gatccttaaa atcttcattc ctgatcctcc tcaaataaaa aatcttgatt aagcagctgc
    17701 ttggcttttt caagtgtttc ttcttgatat tccggcagtt cgctcaatat tctagccgct
    17761 tcttgatgac tatatacttc atttaaaatt tgttggaaat cttgaggtgt ctgatagtat
    17821 tgaaataact gttccatcaa tttagcagaa gcagcaagtg gggtcttcgt ataaaaagta
    17881 ttctctatga gagaaatacg ccaaataaag aaatcattta tcctatctga gaattcctct
    17941 aacaaatagt ctaatagctc tcctgattgt atccgttcca aaacatctgt attgagatgg
    18001 tcataatctt ttaattttat ctcaattaaa ctaaatccat cctctacttt tgaaagctgc
    18061 tgacgaatgt gaacaatgac ctcttttgtc gttcttgctt tcctcaatga tatttccttt
    18121 gtcttccaat agacctctgc tactttaatc ggatgtattt ggcaacttcc tttacttaat
    18181 tcaactagta gaatagatgt agaagcttgt tcctttttag tatgaccttg aggcgcacct
    18241 ggataaacaa ttgtttgctt ttcatttaag accgttggca catggatatg ccctaaagcc
    18301 caataatcgt atcccttttc ttgcatcttt gagggttgga aaggagcata gtttcctttt
    18361 tgagagacgc caggttcccc gtggtacaac ccaatatggt aatctgtgag ttctctagaa
    18421 ggaaattcca tcactttgtc tttttgaatc cacggttgac gataactaaa tccactgaga
    18481 gagacagttt ctccactttt tatcgttatt ttttttgtct ctaccgtttc ggatgtaaat
    18541 aaatggacat ttttaggaaa ggagaaccaa taccgctctt tttgatagaa atcatgattt
    18601 ccgaatatta taaatacagg tatatggttc ttttctaacc gtttcatctg ttctgaaaaa
    18661 tgtttctgta tttttaatga aggacgattt tgatgaaaat tatctcctgc aagtaaaaca
    18721 aaatcaacgt tatttattat tgcctgttca atgatatttg ataaaacggt cagattcgtc
    18781 ttcaatagtt tttcctgtac agttttatca agagttgtca gtccttcaaa tgaccgatcc
    18841 atatgtaaat ctgccgcatg gataaatttc accattaaaa atttcctcct ttttttcttt
    18901 tatcatacca ttttcaatgt tttttttcat caataaccga aaatttgttc gtattatgca
    18961 aagaaattac gataaatggc cgacttcaaa aaataagttt attcgaaaaa aagattgtga
    19021 catgattttt gtcacaatcc taaatatcta tgttatattc gcttcgtttg acactgaaga
    19081 agtactattt gtactggaat ttatgctcca tttttccgta tttagaattc tatgttctat
    19141 actaccttaa ttagtttata tggtaagaat tctttcaaat gcttgagata ctccgtcttt
    19201 atcatttgaa acagtgacat aatcagcttt tttcatgatt tcttgatgac tatttcccat
    19261 tgcaaaacta atatctgcac tttcaaacat agataagtca tttaaactat ctccgatcgc
    19321 gattctcaaa ggtttcttta aattgtagat tgattccaaa cgatcaaaca ccacaccttt
    19381 agatacattt tgaggtacga tttc
//
LOCUS       DACDBG010000049        19104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDBG010000049 DACDBG010000000
VERSION     DACDBG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.049"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(119..1687)
                     /locus_tag="IVZ90_001755"
     CDS             complement(119..1687)
                     /locus_tag="IVZ90_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8262800.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1691..1972)
                     /locus_tag="IVZ90_001756"
     CDS             complement(1691..1972)
                     /locus_tag="IVZ90_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8262801.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1969..2892)
                     /locus_tag="IVZ90_001757"
     CDS             complement(1969..2892)
                     /locus_tag="IVZ90_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8262802.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2889..3767)
                     /locus_tag="IVZ90_001758"
     CDS             complement(2889..3767)
                     /locus_tag="IVZ90_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8262803.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3754..5091)
                     /locus_tag="IVZ90_001759"
     CDS             complement(3754..5091)
                     /locus_tag="IVZ90_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8262804.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5094..6197)
                     /locus_tag="IVZ90_001760"
     CDS             complement(5094..6197)
                     /locus_tag="IVZ90_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8262805.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6271..7779)
                     /locus_tag="IVZ90_001761"
     CDS             complement(6271..7779)
                     /locus_tag="IVZ90_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8262806.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7745..8437)
                     /locus_tag="IVZ90_001762"
     CDS             complement(7745..8437)
                     /locus_tag="IVZ90_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8262807.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8459..9433)
                     /locus_tag="IVZ90_001763"
     CDS             complement(8459..9433)
                     /locus_tag="IVZ90_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8262808.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9733..10605
                     /locus_tag="IVZ90_001764"
     CDS             9733..10605
                     /locus_tag="IVZ90_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8262809.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11187..13385)
                     /locus_tag="IVZ90_001765"
     CDS             complement(11187..13385)
                     /locus_tag="IVZ90_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8262810.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13617..14609
                     /locus_tag="IVZ90_001766"
     CDS             13617..14609
                     /locus_tag="IVZ90_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8262811.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14669..16090
                     /locus_tag="IVZ90_001767"
     CDS             14669..16090
                     /locus_tag="IVZ90_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8262812.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16083..17582
                     /locus_tag="IVZ90_001768"
     CDS             16083..17582
                     /locus_tag="IVZ90_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8262813.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17621..19015
                     /gene="uxaC"
                     /locus_tag="IVZ90_001769"
     CDS             17621..19015
                     /gene="uxaC"
                     /locus_tag="IVZ90_001769"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8262814.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5750 a         3888 c         3370 g         6096 t
ORIGIN      
        1 atctaaaaac attgatgcat aagattgata acttttttta cataacaata tatacaaaca
       61 aaaaaattca ctaactagtg aaaataagtt agtgaacctc atttattaaa gaattaaatt
      121 acagatgatc ttttccatca tacttgaaaa actcaaaatc agcataactt cctctttttt
      181 tatttagatc atgcactgct atcccgataa aatttccagt aaaccctcct gagagtatac
      241 acatgttttt ttgcggacct actttcttcc attcttcagt tgatacgtct ttcaaatgaa
      301 actgtgctgt ctctttcttt acagtgactt tcaaaaaagt cttcttttct gtgagtggaa
      361 tcgtccaatc ttctatttgt gctactccgt tttctgattc tatcatacgt aggcattttc
      421 ccttgtcctc attccaagta acataacaat aaatataatt ggattcattt aagtacagta
      481 ccagtccagc catctgatta aaattattag gcatgtattc aaatgctgtc tccgcatcaa
      541 aagtaaaatc cgtttgccga atagctaaca aatgatgttc gaaggtagat tgaagagatt
      601 caccggaaat cagacgtaaa aaacctggtc tacattttgt atcacaccaa tcagcatttg
      661 gcaaaatgcg tctggcattc cagttcttct ttagctctcc ttcaaatgta tccataaact
      721 gatgatcttt ttcttgttgt acctcttcat ttgtctcaat cattatttct tccatcggag
      781 ttgtctggct atctttaagt cttaaccaat cgtcggacgt ccaatacact tcttgaatag
      841 cggtttctct tcccaaaata gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt
      901 accatcttcc tgacggggtt tgtactaagc tcccatgtcc tgtacattgc aatgtagaag
      961 caggtttgtc actagctgtc agcataggaa aaccaggatc aagctcgtaa ggaccagtaa
     1021 tattctttgc tcggcaaact gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca
     1081 tcaaataata atacccgtta cgaaaataca aatgtggtgc ctccgttttc gctaattctg
     1141 tacctgcaaa tattttatag atgggaccat caagcttctt cttctctgca tcatattctt
     1201 gtaatacgat accgctggat ttattcggtg tgtccagacg ataatcccat agttcattga
     1261 caagccattt ttttcccgtt tcaagatcat ggaacaaaga aggatcaaag ccgttactat
     1321 ttaaataaat tggttcggac caaggacctg tgatctcaga tgcagtgatc aaataattgt
     1381 ggacatcttt aaacggacgg cctgcacttt tgacatctgt gtaaacaaga taaaacaatc
     1441 catcagcata actgatctgt ggcgcccata tacttccatt tgtaggattt ccttgcaaag
     1501 atacttgagt ggtcaaaata tccgttgtat gtgaccagtt gactaaattt ttcgactcat
     1561 atactcggat tcctggaagc cattcaaaag aggagacaat aatgtaatac cgctctttca
     1621 ctcttaatat attaggatct ggattgaatc ctcttaaaat aggatttttc actttgatag
     1681 tctgcatgat tcaggctcct tttctcattc ttactcgata taaactgaat gccccagctg
     1741 ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag accagtcatg tcctgtaata
     1801 atccaaatgt attttctaaa gacaaagttg aatgatctac agcaagcgac tgataacttt
     1861 ttttaaactc tgacgcagac atattactta aagtaactgc taacccgcca aagaaaatca
     1921 aagatagact gccaccgacc attcctagca atagttccca ttttcttttc atttctgaac
     1981 cccctgcttt tctaaatgta tccattcagg tcttttgatc gatgtttcat aattgttatt
     2041 acgttcagag aatttgacag tctgttcatt tttcttgtca tcccaattat cccaaccagt
     2101 tgggtgaaca tgcccaccta tcttacacgc ttcaaataag acatttgcat acggacgcca
     2161 cggtcttcct aaatagacgt ttctagtatc aggttctgca gtcaaggtac agctttcaaa
     2221 acgataccct gttttttgat ttttcggcgt agatgctgct gtgatgtatc caggattttt
     2281 actggctaag ctatggatat gacactgatg aaaaacggca tctgccccac caaatatgaa
     2341 atccaccgtt ccttcaatcc agcaatattc ataggtctga cgacaatact caggattggc
     2401 agaagccggt gtacaaaaag gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca
     2461 taaggtatcc tgataaccag atagtcgaca gttttttaaa gatacgcgat ctcctgaatt
     2521 gaataaagca acagcctgtc ctaccttttc tcctggacct gctgtatttt cgatgtgaag
     2581 attttctaat acgacattcg atccttctaa gaaaaaagta gcacttcgga aagtccctaa
     2641 ctggctaccg tcaagatact cctgttttgc aaaacgatta ccggagataa ctactgttcc
     2701 tgctcccaca agtttaaaat tggataatct gctttctata tattcatcat atcttccttc
     2761 ttttactatc aagacctttg gatggctatc cggcaggcct gccaaataat cgattccttc
     2821 ttggatagtt tcaaaatccg tcggggtatc tatagcattc tttccaatta tcagtttgtc
     2881 tttcatcctc ataaatgctc ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt
     2941 tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt ctggactacc cattgattga
     3001 tactttttgt ccagcccttt tctcagcttc agtacgcctt cgtccaagca catcgtatcg
     3061 ttttttccaa ctaagctaag acgagggcgc ggagcgattt tttcttggat tgctaacgtt
     3121 gtataagctg tcaatagatt tggcacatag taataaaacc catgatgatc taggcggcga
     3181 tgtagcaaaa gtgtttcgat gtccacctgt gccgctagat caatacaaac tttgacacga
     3241 gaatccaatg ctgtcaccca ccagcttaat aagcctccca tagaaagacc aatcgttgcg
     3301 agccgttgag tatctgtgtc ttcccttgtc tccaaataat cgatcaagct gatcacatca
     3361 taaatacgca ttccccataa cgtttttcct gttaataaaa attgtttgaa caactcgctt
     3421 tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa tcgaccaaac agcatatccc
     3481 attcctgtca gtacttctgc aaaagatgga gaagctaagt aaggagcacc atggatcaat
     3541 tcacttttcc cgactgaaaa atctccgccg tgagaatgca ggtaaaggac cgtcggaaac
     3601 ggtccttctt tttctggata agcatagagg gctggtactg tttccaattc atttaagtac
     3661 aattgatagt attcaagcat aaaaccgttt tgtttttctc ggtataatcg ttttccacta
     3721 ggggtatgtc tgctagatgg agcacctaaa aaatcattca actgcataag caaagttttt
     3781 acgaacaacc agttcatcca tattttctga ttgatttgat tggaatttct caatactgat
     3841 ttctttagta ttttctaata gaaatgctgg tccctcgtga ttttcaatcg acatctggtt
     3901 aaaggaaacc ttacccgcaa agccaatata aaatccttgt ctgctcattt cctctattcc
     3961 tgtcatcata gcaggaacac cgggaactgc atcttgggcc atcgagacat cgatattgtc
     4021 aaatgtgatt tcttgtatga attgttccgc taatccataa ataaaacctg cagaagcatg
     4081 gacattcctt gccgtgatat tcgaaaaatg gatccttctg aatgctgatg tccgttcatc
     4141 tatcggataa gccttttttt cccatacata cggttctttc cctcgcggac cacagaaata
     4201 ataaagattc agtataaatg gacacatcac attatccatg actatattgg atactcgaat
     4261 atcttcaacg atcccgcctc gacctcgtct agatttcaat cgtattcctc gatcagtttc
     4321 ttggaagata caatttgaaa tggtaatatt acgaatacta ccactcattt cacttcctaa
     4381 tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc
     4441 gatcctttcg tacgtatctt cagtccctgc cttgattgca atacagtcat ctcccacgtc
     4501 aatatggcaa ttactaatac gcacattttt gcatgattct ggatcaattc cgtctgtatt
     4561 tggtgaatcc gctgggttca agatcgtcaa gttatcaaac gttgcattac tgcaaagaat
     4621 cgggttgatc gtccaacttg gtgactggat caatttaata tctttcacag taattcgatg
     4681 acagttatgg aaactcatca atttaggacg aggataagcg agattatctg gttcatttct
     4741 aaaagtatgc caccattttt ttccatttcc atctaatgta ccgaaaccag tgacagagat
     4801 gttctctaca ttttgagcat agatacagga agcataaact tttcgatgga ctccttccca
     4861 acgtgaatga acaacaggat aatcttttgg atcgtcagag aatttcagaa ccgctcctgc
     4921 gcttaaatga agttcgacat tgctttttaa aaataaagct cctgttagaa attcacctgc
     4981 tggaacaact acggtcccgc ctccgtcact tgctgcagca tcaatggctt gttgtattgc
     5041 ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa atatcataca tatttataat
     5101 gcctccactt caatcaatgc ttgcaagaat gcaccaacgc ctttttgatc attacaaata
     5161 attggttcac tgatgtagta agcatatgaa ccatctcgct gatctgctcc gcctaaccct
     5221 gctacttgac agtttttatt caagttcagc cagccttctt ttgtttccag aacaaattca
     5281 tcaagtaatc cttgatgtga tttcttcact tgtggaagcc attcttcttt gttcagcagt
     5341 cccaaacgta ttcctttagc cattgcgtaa acaaacatac tactgccgga agcttccaga
     5401 taatttcctt tccgttttgt ttctgttgtt acttgatacc atgtcccgct gttttcttct
     5461 tgatatggaa gtaatgcagc cagtgtcttt tctagaattt ctttcagcaa agaacaatct
     5521 gtttcttcat gaagtaattc ataggtatct gctgcagcca tcaaatacca gccaatcgat
     5581 cggctccaaa aatgatggga tagacctgtt tttggatcag cccatggttg gaccttcttt
     5641 tcatcccatg catgaatcag caaatttgtt tttggatcga atagatgttt ataactgatt
     5701 tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac taaaattctg gataaattca
     5761 gcataaaaag gagaacccat atataagcca tccaaccaaa tttgataagg ataaatttct
     5821 ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt
     5881 tctgctgctt ttttatattt ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt
     5941 cctgtattta catggtcgat attgtattct tcaacgctgt agccacggat cgttccatct
     6001 tcttgcacga aatagtcgat attgtctttt atgaaatcaa aataaatttt ttcttgggtt
     6061 cgttcccaaa gtaattggaa ccctttaagt acgacgccat agtcataaga ccattttcct
     6121 ttataccact tttcttcata taagcgaggt gttctttcca tgatactttt tgctaatttc
     6181 tttgaccagt tttccattat ttgctctcct ttaaaaatta aaataggatt tgggacaaat
     6241 accaagaagt atttgtccca atcgtctata ttactgtgcc gctgctttgt atgcttcctc
     6301 gtactcttgt gtgattcgat cgcctccttg tgctttccag tcttctacag cttttttgaa
     6361 tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta gcatcaatga cgattttttc
     6421 aatttctgat cctacttcat tgttcgtcgg tgaatccaat ggcacagaag gatccattac
     6481 agcaaattct tcattttctt tgatcaattg attagagaat tctttttcag ggtctgtatc
     6541 tttgaaagag aacgtcactt cactaggacg agagcttgat aacggttgca cctcttgttg
     6601 ccaaagatcc atatcgataa attcgataga accatcatca ttcaattcat aatgcgttcc
     6661 ttcgataccg ccagtcatca atttatacac atcttcttct aaaagatcat caacaaattg
     6721 aagtaatttc ttcaattgtg cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc
     6781 gccaatacca tttccttctg accaaacatg ataaccgccg ccttcaccat ctgagatctt
     6841 gtttacaggc actaattcca tatcatcttg aataccttga cccatcgtct tcagattctt
     6901 gatatccacc attccagtgt agattccagt tcttccttgt gcaaattgtt cttgctgatc
     6961 agtcttttgg gtaactgcaa agtcttgtgc caaatagcca ttctcaaata gatctcttga
     7021 aaaattcaaa gctttcatat actcttctgt atcaaattct ggagtgaatt tgccattatc
     7081 gtctacagcc cagccatttg gtgctccaaa ataagaagtg aaaagtttaa agctggtata
     7141 acgaatatca ctaggcgcac gatcgccaaa accaactgtg tcgtccacac catttccatc
     7201 aggatcgttt tctgtaaatt gtttagctac ttcataaagc tcatccatgg tagttggaac
     7261 atctagtcct agattatcca accagtcttt tcgaatgact aagccagcac gagcgtagtc
     7321 tttttgtatc ggtacgccgt acaaggcacc gtcgatactc gcagcttcga ttctatcttc
     7381 agaaatttga cttaaatttt tatagtcttt taaatattct gatacatccc agaatacgcc
     7441 tgattttaac gcattgcgga cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat
     7501 atctcctagt tcatcagaag ccaatgcagt tgttaatctt tcttcttttg aagcatcagg
     7561 aatccagttg aatgtcagtt ccgtatcggt tttttcttcg atcaagttca caatcgtgtc
     7621 tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg attttagctt ttccatcttc
     7681 tgtctctgaa gaactgttac ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc
     7741 aaaactaaga cctaatttta cgagcgttgc ttttttcata aattttctcc ttcactgttt
     7801 taataatatt tttgttttct tctgtttttt ctgtatatga gttcttccat tccaaatggc
     7861 aaaatataga atttgctgtc cacgtgactg cggaaaagaa gagactgcca ccgataaagg
     7921 ttaaaacacc tggcattgtt tggatgatca tcagataaat ccctaccata cttaatatca
     7981 acactaatga gtaatgaaga tgtgagaatc cgaataaaaa tgccattttc attttgaaaa
     8041 aatatccttt ccaatcataa tgagccatga ttgggaaaat atagagtgct gagagcacat
     8101 atatgaacgc agcaatatac aaagcgatcc taagatacca attgtgtatg aaagaaagat
     8161 ccacaagtaa aacaatcccg atggcgccat atatccagct catcagcaag gtttcacgat
     8221 agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca
     8281 gccattttct aatcactgca accatcgcat acgatgcagg accgaaagta aaaactccca
     8341 aacctgcgat gactaaaccg ccccacagaa aattcaaata aaccaactgt aaaatacgat
     8401 ttagccttcc gtttatttta tctaatgact gcagcatatc tttccctctc tttctatctt
     8461 agtaaacgcc gtcttctcct agctttttgg caatcttatt agctaaaatt accataaaca
     8521 atcctacgac ccctttaaac aatcccattg ctgtactgaa acttaactgt ccattcttca
     8581 atccagctgt ataaatatag gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca
     8641 ataacaagta catatgctcg aaccctaagt ctaatgtctg accgattttt agaatcagca
     8701 aggtaatgat tactggacga attgccggca atgtaacatg ccatgttttg cgtaagcgtc
     8761 ctgctccatc catttctgca gcttcataca actgtgtatc gactgcagtt atcgctgaaa
     8821 ggtagatgat cgtagaccag cccaactctt tccagatcac ttgtccgata tatactgtgc
     8881 gtgtccattc aggggatgtt agaaaactga ttttttcaaa tcccattgtt gcaaggaatt
     8941 cattcaatac ccctccgtca acattcaaaa atacataagt aatcgaaaca atgattaccc
     9001 aagacatgaa gtgagggatg taaatgatcg tttgaatccc gtttttcaat cgtttgttcg
     9061 ttacttcatt taatagtaaa gacaaaatga ttggcatcgg aaaaaacacg aagatattta
     9121 atccgaacaa gatcaacgta tttcttaata atgtaaagaa tgtcggttct gtaaacaaac
     9181 gaatgaaatg tttgaaaccg acccaaggac ttcccattat tcctaaaaat ggttgataat
     9241 cttgaaaagc aataacgagt ccccccatag gaagatacct aaaaatcaag aaataaagga
     9301 ctcccggcag aatcatcaca tataaaaatt tattcgtttt gattcctgca attatttttt
     9361 ttcgtcttct gagcttttct tcttttttcg atagcgtcag ttgaaatggt tcttgtttct
     9421 ttacactttc cataagcccc tccttagttt tgtgaatgtt tgctgcttat gacttcatga
     9481 tagataacta tctgaattac gtatataatc atttttatcg taagtaaaaa cgcttacata
     9541 gcaatgattt ttgtgctata caaaacgata aagtgtattt tttcacaaat aaactacgca
     9601 tagcaaacct tctatttttt tctatctgac acttagcata ggaaatgatt atatacgtaa
     9661 tctttcaccg atgataaatt gaacatatag aaacagaaac atttaattta ttttgaggag
     9721 gagcctatct atatgaaaaa gaaagtctct ataggagaaa gaagttttac acttttcaac
     9781 aatacgtttc taattttgct agctttgatc tgtatcgttc cttttttaaa tatcatcgct
     9841 acatcttttg cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc
     9901 ttttctttag acgcgtatcg ctacattctt tctacgccga ccatttttag agcacttgct
     9961 gtttcgattg gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg
    10021 gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta
    10081 ttttctatgt tgttcagcgg ggggatgatt ccaacgttct tagttgttcg ttctctaggg
    10141 ctggtcaatt cttactgggc catgatcctg cctgtggctg ttaatgcaat gaatatgatc
    10201 attatgagaa actttttcca agccttgccg gatagtttgg aagaatcagc aaaaatggat
    10261 ggttgcacag attttggtgt atttttcaaa attatgctgc cattagcttt accgtctatt
    10321 gctactatct cgctttttta tgctgttact tactggaaca cttatatgac tgctattttg
    10381 tatatcaacg attcatctaa atggccgata cagattcttt tacgccaaat cgtcattgta
    10441 tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc acaaacagtt
    10501 aaaatggctg ttatcgttat tgcaacagtg cctatgctga ttgtttatcc ttttgttcag
    10561 aaatattttg tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg tactgaacgc
    10621 aaaatggtag ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat
    10681 tgcttcttaa atttttgtga aatttcggct tatttttcga agaatctact cctggaacac
    10741 cgtgtatcag aggttgtgaa agagacgttc agccttgagc gatgataaaa actctcacaa
    10801 acattgcgac gagtaaccca caggagcaat aagaagcgat tttgaaaaat agcttcccat
    10861 atttcacaaa attatgcctt attgtcgata gcctgtctct tgaacgccat ttattcgtaa
    10921 aaaagaactg tgacaagact tttgtcacag ttctttttgc gtgcttagcc ttgaaattta
    10981 actatccata aatacaaata ctagggttat tctaacttct tttattgctt gaatcagtcc
    11041 acaagaaaaa cagcctcttg attaaacggc gtttatcttg ttgattctgc ggcaataaaa
    11101 tcagccagca gcaaaacatg aagtgctgtt ttttgctgtc ctcttttatt gcttgaatca
    11161 gatcacaaga aaaacagcct cttgattcac aaatattttt ttcgatattc tcccggcgta
    11221 atcgattcct tctttttaaa gaaacgaata aaattttgcg gatttcgata ttgcaatctt
    11281 tctgcaatct cttttacgga gaggttagtc tctttcaacc atttttttgc tacctctaaa
    11341 cgatgatttt gaacaaaatc accaaatttt tctcctgttt cctttttaaa tacattactt
    11401 aaatagttag ggttataatg caacctatca gcgatcacat ctaaagacag atcacgatca
    11461 tattctgtgt agacgatatg cataatattc tccgacaaat ttttaaattc atgatcagtg
    11521 attgcttgag agcattctgt gattggcaga actaattgtt ctaaaacaag tttttctagt
    11581 tctttgggat gataattgtt cagtgctttt acataaattt gtttcatgtt atcaaagttt
    11641 tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa
    11701 actacttccc gactcaaagg atttttattt tgtgcaaaca atgcatcaat caattcatgt
    11761 actaaattct tcacttcctg atttccagaa cgaatggctt caaacaatcg attttgttct
    11821 tctatcggat atctaataag tgttttttca tgagcagcag gaacgatttc ctgataaaaa
    11881 ataattgaac ttggaccagt atttacacga taatacaggg cttcttttgc ccgatcaacc
    11941 gattgcttgc tttcattcag cgtttcgaac cggttactga tgcctatact gatagttaaa
    12001 ttcaagtatt cttttacacg tttttgaatg agtcggcaat actccatgac ttttttgctt
    12061 gcttctgcat catctttatc cattcgataa atagtggctt gcatttcttc attcaaaacg
    12121 atcggcagca ttcgattttc ctttggcaca atttccgtga tcatgttgtt gatcgctaaa
    12181 agtagcaaat cacgctcccc accgtgacta tcttctaaca gatcgatttg aaccaaagct
    12241 gtataatagc agtcattttg agaactataa ccaaattgct gcaaccgctg atctagttct
    12301 cgcctttcta ctcgatttct aaataaacta agtacaaata gcgtttccag ctgtggcttt
    12361 tgggtaacta agtgtgcact taacgcttct ttttctccta tgatccgact gactgattga
    12421 gcgaccaaat ctagttcttt cccactgaat ccttttgatt taagattcag acgttcctgt
    12481 atttgagaaa tcggacgggc aaatctgtcc gaaaataagt aggaaaaaag accaatgagt
    12541 cctaccagtg ttaaagacac tgcaactagc ccgattctca gattgcggat cattgcgcca
    12601 attgcactag agtcaacttc taatctgtaa atccaattat tataatctga tttcagtact
    12661 atatacgttt tatctttttc tcttatcact ttcagctgat tgtaatctag atccacgtcc
    12721 gttaatacag ttggttgctc tttatttttt actgtctgag tagtaaaaag gttgccaact
    12781 tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa caacttgttc tattgtatgt
    12841 ttctctatcg tagcaattcc caatgcatat ttttcttttt caaacattgg caaagaaatc
    12901 accatttcga ttcctttgtt tttcttttgc caaaataaat tatttttgtt atttatgtac
    12961 ttctgttgat acttttcaac ttcctcttcg gtcagttgac tcaacgatcc attgattact
    13021 ccccatttac cagccaaact gactaaatca taattattat catccataac aatcgtttct
    13081 ataaaattta gttctttcct gatttcagaa taagtggagt aatctgtata tgtcagcgga
    13141 tgcttgaata tgtccgagaa atttttggtc gaaccatacg tagaaaacga atattcaatc
    13201 gttctaattt tccattcaac gttagtttta acttgcctga gcaaattctt tttgtctgtt
    13261 aaataactcg attcgatcga ttcataggta ttctggtaaa tgaataaact aaatcctatg
    13321 acaagtacgg ctcctaataa aaacatcggt aaaaatattt tataaaatac tgtacttttc
    13381 ttcattttct tcctctcctc cttttaaaat aaaaggcttt catttttctg taaaattgat
    13441 actcatctaa ttaaatgagc attccccgct ttcaagaata gcataattaa aaataaaaat
    13501 ccaagtaatt tttaacgtgt gcaatctttt catatcaaag atctattgac ttatcgtagt
    13561 tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg
    13621 taacgatcaa agatatcgct aaagcagctg gcgtttctca tacgacagtt tctcgtgctt
    13681 tgaataacag cagtttaata aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg
    13741 aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa
    13801 tcggactatt tttctcaagt ttgcataaag gaacttcatc aaatttccta gcagattcca
    13861 ttgcaggcat ccattcagta ttagacacaa attacagttt atctgttgaa agtatcgatg
    13921 ttgttgattt aacagaaatc aacctgcaac gctacgatgg actgatcgtt atgagtcaaa
    13981 gtgatgatga tcagcctttc atcgactatc tcaaagaaaa agaatttcct ttcgttgttg
    14041 tcaatcgtca catcaatgat ccagcggtta tcaacgtcac tgccgatgat gcggccggtg
    14101 ttatgcaggc agtagattac ggtattggat gcggacatac gaagatcgct tatattggcg
    14161 gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa
    14221 aagcgagcat ccctgtcaat ctagcctatt ttttaacagg agattacagc ttagaaagcg
    14281 gcctccaaaa catggaatac ctattatcac tttctgacct gcctactctg gttttttgcg
    14341 gcaacgacga catagcgatt ggtgcattga gagctgtaca tttagctggc ctccgtgttc
    14401 cccagaacat ttctctgatc gggttcgatg attctccagt cgtctcctat ctcaatccgc
    14461 cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca agaaggaaca aagcttttac
    14521 tagatttgat cgaaggcagg acgatcgaac aatctaaaat tgagctctcc accttgttga
    14581 tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa ttttatataa aaatgttaac
    14641 gtgtgcataa cacagaaagg aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa
    14701 aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc aattttatgc gtggatttat
    14761 tgattggcag attcagcagc tgaataacca acaattgttt aaaggtaatg ttgcaatcat
    14821 tcaaccttta gaacaaggtc ttggaaatat gatgaaccag caagataatt tatatacggt
    14881 cattttacaa ggactcagca atggcgaaat cgttgatact tccgagatta tcaccgtagt
    14941 agaacaaatg atcaatcctt atgatgaatg ggatgcttat ctatctctag cagaaaatga
    15001 cgacttggcc tatatcgtct ccaatacaac agaggcagga atccaattcg tggagactga
    15061 taatttaaca gatactcccc cacagagctt cccgggcaaa ctgacagcct ttttataccg
    15121 acgtttcaaa ttaaacaaag ctggctttac catcattcct tgcgaactga ttgacagaaa
    15181 cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa aaatgggatt tagaacctgc
    15241 ttttgttgag tggttagaaa aagaaaatgt tttctgttgt agtttagttg atcgcatcgt
    15301 cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa cggcataact atgaagatca
    15361 attgatggtc aaagcagaac cattcatgct ttgggtgatc gaagggccta aagaattgga
    15421 gaatcaacta ccattcaaac aggctggctt gaatgtcatc atcacagatg atatgactcc
    15481 ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat acagcgatgg tccctcttgc
    15541 tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa gatcctttat tctccgcata
    15601 tgttgatcag ttattcaatc tagaattgat tcctatgctt tctttaccta aagatgaatt
    15661 agccatttat gctgaccaaa tcaaagaacg tttcttgaat ccttttgccc atcacaaatt
    15721 agaagcgatt tcattaaata gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa
    15781 gaagtatatc gaagaacaaa atcaggtacc accgttaatc actgtttcac tagctgcgct
    15841 acttcttatg tatcgaggcg atcaagtaaa gccacatgat gatgaaaaga cgataagcga
    15901 atttactgat gcttggtctg ataatggaac agctattcct cgattattgc aaaacgctgc
    15961 gctttgggga gaagatttgt ctcaaatacc gaatgtgaca gatacagttc aagaaatagc
    16021 tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa gaaatgaaag gaacgatggt
    16081 ccatgcttga aaccctgcaa ccaacaatga aattaaatcg acgagacagt gtggtcgtcg
    16141 cattaacccc tattccaaaa caaacacaac tgacgattga tcagcaaacg atcaccactc
    16201 ttgaagatat cccgcaaggt cataagattg cattgggtga tttgaaagct ggagacaacg
    16261 tgatcaaata tggctatcca atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc
    16321 tgcacactca taatgtaaaa accaatcttt ctggtgaatt ggattatcga tatgaaaaag
    16381 atgtccatcc aagtcattac ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa
    16441 atggaaaagt agggattcgt aatgatttgt ttttagtacc taccgttggg tgtgtgaatg
    16501 gcattgctga actgatcgtc aaacagttca aggaaaaaca tccagatctg ggacagtttg
    16561 accatattac gattttaaag catccttatg gctgttcgca gcttggaaaa gatcatcaaa
    16621 atactcgaga aattttagcg gacgcagtaa atcatcccaa tgccggagga gttttagtat
    16681 ttggattagg ttgtgagaac aatacagttc ctgaattcaa aaaaatttta ggtgaatatg
    16741 atgaagaacg agtgaaattt ctagtggctc aagatgttta cgatgagatc gaacaaggtg
    16801 tggctctatt agaagagctt ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc
    16861 tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga tggtctatcg ggaataactg
    16921 ctaatccttt acttggtgca ttttctgact acttgattgc tcaaggaggc agcacgatct
    16981 taacagaagt gccagaaatg tttggcgcag aacaagtatt gatggcacga gcggaaaatg
    17041 aagaagtttt tgactcaatc gttgacttga tcaatgactt caaacagtat ttcttgtctt
    17101 acggggaacc agtttacgaa aacccatctc ctggtaataa agcaggtggg atcactacat
    17161 tagaagataa gtctttaggc tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc
    17221 tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt attgcaagct cccgggaatg
    17281 atcttgtagc tgcctctgct cttgcttcat ccgattgcca gttggtcttg tttaccacag
    17341 gaagagggac tccgtttggc tcatatgtcc caacggtcaa ggtatctacg aatacaactc
    17401 tttttgaacg caaaggtcat tggatggatt ttaatgcagg cgtcttatta gaacaaccaa
    17461 tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa
    17521 cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt taaaaacgga gtaacacttt
    17581 gattttcata aaacagtata aaaagaaaga aggaagattt atgtttttat ctgatgattt
    17641 tttattaaaa gacgattggg cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat
    17701 tattgattat cactgtcact tagatccaaa agaaatttac gaaaacaaca atttcagcaa
    17761 tctgacagaa gcttggttag gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg
    17821 cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt gaaaaatttt tagcttggtg
    17881 tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt tggtcacatc ttgaattgaa
    17941 gcgtttcttc ggtattgact tgttgataaa tgaagaaaat gcagagaaaa tttggacaca
    18001 agcaaatgaa attttggcag ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa
    18061 tgtagaagtg atttgtacaa cagatgatcc cgcagacgac ttgcactatc atcagttgct
    18121 tgctcatgaa gaaccagatt tgaaggttct accttcattc cgtccagata aagcactcaa
    18181 tattgaaaaa gaaggattta atgcatgggt cacactactg gaagaagcag ctgaccaaac
    18241 gatcaccacc tatcaagaac tgatagaagt cttagggcag cggatagaat acttccatca
    18301 acatggatgt cgtgtttccg accacgcttt agacatctta cgctataaag aagcggatga
    18361 aacagtatta gaagagattt ttcaaaaagc acgagctaac aaactattat cttcagatga
    18421 aatcgatgct tatcttacag aaacactgat acatttgacc cacttctatc attctcataa
    18481 ttggacgatg cagctgcaca tccatgctta tcgtaactgt aatacacaaa tgttggaaaa
    18541 attagggccc gataccggct atgatggaat gaatgatctt tctttgacta ttcctctgca
    18601 aaaattattg aaccgtgcag aagaaacgga tcagttgcca aagacgatcc tttattcatt
    18661 gaatccgaat gactatcctg cgattttagc tttgatgggc tgtttccaaa aagaacagaa
    18721 tggaaaactg cagttaggtt ctggatggtg gtacaacgat actcgagctg gtatgcgcga
    18781 tcaattgaca caatttgctg atggtagtgc tttggggaat tttgtcggca tgctgacaga
    18841 ttcaagaagt ttcctgtcct acactagaca tgaatatttc cgtcgtgttc tttgtgaatt
    18901 tttaggcgaa atggtcatgc gaggagaagc acctgaagat gaacaacttt taggaagtct
    18961 agttcaagcg attagttata caaatgctaa aaattacttt ggcttttttg cctaattgat
    19021 taatagtaac agtaacgaat caaaaacagg atagtgaaat aacggaatcc gtttatccac
    19081 gttttagaga agaagacgga ccaa
//
LOCUS       DACDBG010000050        18410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDBG010000050 DACDBG010000000
VERSION     DACDBG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.050"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(105..560)
                     /locus_tag="IVZ90_001770"
     CDS             complement(105..560)
                     /locus_tag="IVZ90_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262815.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IVZ90_001771"
     CDS             complement(714..1922)
                     /locus_tag="IVZ90_001771"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8262816.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IVZ90_001772"
     CDS             complement(2034..2366)
                     /locus_tag="IVZ90_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8262817.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IVZ90_001773"
     CDS             2514..3389
                     /locus_tag="IVZ90_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8262818.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IVZ90_001774"
     CDS             complement(3471..4052)
                     /locus_tag="IVZ90_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8262819.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IVZ90_001775"
     CDS             complement(4058..5317)
                     /locus_tag="IVZ90_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8262820.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IVZ90_001776"
     CDS             complement(5319..6365)
                     /locus_tag="IVZ90_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262821.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="IVZ90_001777"
     CDS             complement(6600..6875)
                     /locus_tag="IVZ90_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8262822.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /locus_tag="IVZ90_001778"
     CDS             complement(7118..8428)
                     /locus_tag="IVZ90_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8262823.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IVZ90_001779"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IVZ90_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8262824.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="IVZ90_001780"
     CDS             complement(9974..10654)
                     /locus_tag="IVZ90_001780"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8262825.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="IVZ90_001781"
     CDS             complement(10703..11338)
                     /locus_tag="IVZ90_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8262826.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="IVZ90_001782"
     CDS             complement(11404..12807)
                     /locus_tag="IVZ90_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8262827.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="IVZ90_001783"
     CDS             complement(12794..13825)
                     /locus_tag="IVZ90_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262828.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="IVZ90_001784"
     CDS             13856..14077
                     /locus_tag="IVZ90_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8262829.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="IVZ90_001785"
     CDS             complement(14242..14979)
                     /locus_tag="IVZ90_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8262830.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="IVZ90_001786"
     CDS             complement(15132..16472)
                     /locus_tag="IVZ90_001786"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8262831.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="IVZ90_001787"
     CDS             complement(16709..18232)
                     /locus_tag="IVZ90_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8262832.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5184 a         3925 c         3107 g         6194 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggccccttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    18301 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    18361 cctattccct cctctttttt cagtttttta aagctttctt ctttttcatt
//
LOCUS       DACDBG010000051        17464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDBG010000051 DACDBG010000000
VERSION     DACDBG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.051"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IVZ90_001788"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IVZ90_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IVZ90_001789"
     CDS             complement(407..766)
                     /locus_tag="IVZ90_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8262833.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IVZ90_001790"
     CDS             complement(796..1137)
                     /locus_tag="IVZ90_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262834.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IVZ90_001791"
     CDS             complement(1134..1808)
                     /locus_tag="IVZ90_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8262835.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IVZ90_001792"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IVZ90_001792"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8262836.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IVZ90_001793"
     CDS             complement(4322..5512)
                     /locus_tag="IVZ90_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8262837.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IVZ90_001794"
     CDS             complement(5533..6060)
                     /locus_tag="IVZ90_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8262838.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IVZ90_001795"
     CDS             complement(6107..8896)
                     /locus_tag="IVZ90_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8262839.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IVZ90_001796"
     CDS             complement(9043..9243)
                     /locus_tag="IVZ90_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8262840.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IVZ90_001797"
     CDS             complement(9696..10016)
                     /locus_tag="IVZ90_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262841.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IVZ90_001798"
     CDS             complement(10519..11637)
                     /locus_tag="IVZ90_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262842.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IVZ90_001799"
     CDS             12108..13415
                     /locus_tag="IVZ90_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8262843.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IVZ90_001800"
     CDS             13536..14735
                     /locus_tag="IVZ90_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8262844.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IVZ90_001801"
     CDS             complement(14762..16030)
                     /locus_tag="IVZ90_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8262845.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            16123..17462
                     /locus_tag="IVZ90_001802"
     CDS             join(16123..16564,16564..17462)
                     /locus_tag="IVZ90_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8262846.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         5195 a         3612 c         3101 g         5556 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagat gatgtgcacc
    16081 ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt
    16141 gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt
    16201 gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat
    16261 catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat
    16321 gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat
    16381 atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct
    16441 tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct
    16501 aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca
    16561 tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg
    16621 ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac
    16681 aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata
    16741 gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc
    16801 gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa
    16861 aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata
    16921 aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac
    16981 ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc
    17041 tatctcctat acttgattta tttaacggag aaataattag ttatagtata tcaactagtc
    17101 caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg
    17161 gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa
    17221 aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt
    17281 ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc
    17341 gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa
    17401 ggataaaact aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat
    17461 agtt
//
LOCUS       DACDBG010000052        17093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDBG010000052 DACDBG010000000
VERSION     DACDBG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.052"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            208..2250
                     /locus_tag="IVZ90_001803"
     CDS             208..2250
                     /locus_tag="IVZ90_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8262847.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="IVZ90_001804"
     CDS             2261..2881
                     /locus_tag="IVZ90_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8262848.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="IVZ90_001805"
     CDS             2893..3351
                     /locus_tag="IVZ90_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8262849.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="IVZ90_001806"
     CDS             3370..3648
                     /locus_tag="IVZ90_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8262850.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="IVZ90_001807"
     CDS             3673..5040
                     /locus_tag="IVZ90_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8262851.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="IVZ90_001808"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="IVZ90_001808"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP8262852.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="IVZ90_001809"
     CDS             6227..6718
                     /locus_tag="IVZ90_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262853.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="IVZ90_001810"
     CDS             7161..8360
                     /locus_tag="IVZ90_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8262854.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="IVZ90_001811"
     CDS             complement(8855..9613)
                     /locus_tag="IVZ90_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8262855.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="IVZ90_001812"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="IVZ90_001812"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8262856.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="IVZ90_001813"
     CDS             10713..12158
                     /locus_tag="IVZ90_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8262857.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="IVZ90_001814"
     CDS             12186..12641
                     /locus_tag="IVZ90_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8262858.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="IVZ90_001815"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="IVZ90_001815"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8262859.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="IVZ90_001816"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="IVZ90_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="IVZ90_001817"
     CDS             14450..15064
                     /locus_tag="IVZ90_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8262860.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="IVZ90_001818"
     CDS             15305..15688
                     /locus_tag="IVZ90_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8262861.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="IVZ90_001819"
     CDS             16051..16560
                     /locus_tag="IVZ90_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8262862.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..>17093
                     /locus_tag="IVZ90_001820"
     CDS             16660..>17093
                     /locus_tag="IVZ90_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262863.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQL"
BASE COUNT         5463 a         2743 c         3609 g         5278 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caa
//
LOCUS       DACDBG010000053        16984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDBG010000053 DACDBG010000000
VERSION     DACDBG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.053"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..382
                     /locus_tag="IVZ90_001821"
     CDS             8..382
                     /locus_tag="IVZ90_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262864.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            612..956
                     /locus_tag="IVZ90_001822"
     CDS             612..956
                     /locus_tag="IVZ90_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8262865.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            961..1242
                     /locus_tag="IVZ90_001823"
     CDS             961..1242
                     /locus_tag="IVZ90_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262866.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1345..1659
                     /locus_tag="IVZ90_001824"
     CDS             1345..1659
                     /locus_tag="IVZ90_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8262867.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1637..3331
                     /locus_tag="IVZ90_001825"
     CDS             1637..3331
                     /locus_tag="IVZ90_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8262868.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3351..4529
                     /locus_tag="IVZ90_001826"
     CDS             3351..4529
                     /locus_tag="IVZ90_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8262869.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4492..5178
                     /locus_tag="IVZ90_001827"
     CDS             4492..5178
                     /locus_tag="IVZ90_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8262870.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5178..6338
                     /locus_tag="IVZ90_001828"
     CDS             5178..6338
                     /locus_tag="IVZ90_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8262871.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6348..7223
                     /locus_tag="IVZ90_001829"
     CDS             6348..7223
                     /locus_tag="IVZ90_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262872.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7220..7531
                     /locus_tag="IVZ90_001830"
     CDS             7220..7531
                     /locus_tag="IVZ90_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262873.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7521..7874
                     /locus_tag="IVZ90_001831"
     CDS             7521..7874
                     /locus_tag="IVZ90_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8262874.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7864..8265
                     /locus_tag="IVZ90_001832"
     CDS             7864..8265
                     /locus_tag="IVZ90_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262875.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8258..8662
                     /locus_tag="IVZ90_001833"
     CDS             8258..8662
                     /locus_tag="IVZ90_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262876.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8674..9282
                     /locus_tag="IVZ90_001834"
     CDS             8674..9282
                     /locus_tag="IVZ90_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8262877.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9302..9664
                     /locus_tag="IVZ90_001835"
     CDS             9302..9664
                     /locus_tag="IVZ90_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262878.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9667..9849
                     /locus_tag="IVZ90_001836"
     CDS             9667..9849
                     /locus_tag="IVZ90_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262879.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9866..13285
                     /locus_tag="IVZ90_001837"
     CDS             9866..13285
                     /locus_tag="IVZ90_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8262880.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13336..14073
                     /locus_tag="IVZ90_001838"
     CDS             13336..14073
                     /locus_tag="IVZ90_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8262881.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14083..16374
                     /locus_tag="IVZ90_001839"
     CDS             14083..16374
                     /locus_tag="IVZ90_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262882.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16398..>16984
                     /locus_tag="IVZ90_001840"
     CDS             16398..>16984
                     /locus_tag="IVZ90_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262883.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         6071 a         2472 c         3567 g         4874 t
ORIGIN      
        1 agagtgcatg gaagacaaaa tgtatttaag acaaaaagtt ttcacaaaaa aagatattga
       61 aaatcaaatt aatggattta tacagaaatt agaaaatgca aattcttatt tgataaataa
      121 ggaactaaat aaagcttatg accagtggaa agaagtatat gatgagttga aattgattca
      181 aaatgaaaca aaattagtac gaacggagaa aaagaatgaa aatagtttct tttttgatgg
      241 atatgctata ttgatgttgg aaactgtagc aaaacaaaat ataaaagctc ccaaaaaaga
      301 actatctgac aatattgata acgcattagc cgagttaagg tattacgtta tgcaaataaa
      361 agatgttagg atcactcgtt gagtggtctt tttattttgc acaaaggagg taacaacaat
      421 gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa atggtaacgg
      481 agagatagta cgaaagtata gaagaccaat aaagagcgat acatataaac gaaaggaaag
      541 caatgaagtt attccattgt atggcaaaag aatagctaag cattaaataa gattgtgaaa
      601 ggagacggga catgaacgag gaattctata gatggctatt acagttgata agagaagatc
      661 gtttggttaa gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac
      721 gagatcacta tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg
      781 ttcatcatat aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta
      841 tttgtttatg tgttgaacat cataatgaag ttcatggcag atatcttaca gcattggata
      901 aacaagagaa gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc
      961 atgatcatca atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct
     1021 tatactcagg gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg
     1081 ttatcagata actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa
     1141 gataataacg tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat
     1201 tacattgact tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat
     1261 cgcaaatttt ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa
     1321 ctttatcatg aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga
     1381 gaaaaattac gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca
     1441 ttcttagata atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa
     1501 gatatatccg agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg
     1561 cttgtttctg tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa
     1621 ttggtaggtg aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa
     1681 agctatagaa gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct
     1741 agaagaaaga gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc
     1801 aatcaatata ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca
     1861 atgttttatt tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct
     1921 ttcattaatg ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct
     1981 actaaccgct aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag
     2041 ccaagcaaag actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga
     2101 taaaaaagta tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact
     2161 tcgttataac acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt
     2221 tgatgaaatt cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg
     2281 taaaattcga gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc
     2341 gcttgatgac attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa
     2401 ggatggagca gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga
     2461 agttgatgat cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcaaattt
     2521 aaaacggaaa atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac
     2581 gttcatgacc aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata
     2641 tgatgatgtt ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg
     2701 aacagtcgat tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga
     2761 taaagatgtt tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa
     2821 catcaaacga gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg
     2881 aaaaaatata gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta
     2941 tattaaaaaa atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga
     3001 agcaggtttt actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga
     3061 tctggttgat gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg
     3121 ttggtattgc atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa
     3181 aatagaacct gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt
     3241 tttagatgat atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac
     3301 aggaactgga tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt
     3361 ttcaaaaggc ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt
     3421 gtaaattgca agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga
     3481 ttgcaaatgc gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga
     3541 atgatcttta ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta
     3601 gaaaaaaact gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta
     3661 ataattctag cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag
     3721 atgatgtgtt ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa
     3781 gtgatgttat ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt
     3841 atcaagcgta tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc
     3901 gtagatacgt actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa
     3961 tcaataaaat gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg
     4021 tatttcaatt acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt
     4081 caagagatat aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg
     4141 ttccgaaaaa cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt
     4201 taatgttcga aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct
     4261 atgaagtaga agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga
     4321 ttactagctt caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta
     4381 caatcaacga aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg
     4441 aaatatttat aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca
     4501 atgaaaacat ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga
     4561 tttattggtt ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg
     4621 gatagtctag gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta
     4681 tttcaaggga ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg
     4741 attaacggct tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa
     4801 atctacaaca atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc
     4861 gatgacttcc gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca
     4921 tataaaacac gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg
     4981 tttatggatg cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa
     5041 aatagctcag gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt
     5101 aaagaaaaaa gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga
     5161 caaggagatg taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc
     5221 aattgatgaa agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct
     5281 tatctaagga aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat
     5341 tagtattaat gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg
     5401 aagtagtgaa aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg
     5461 aacgtgtatt tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta
     5521 acacaactgg tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta
     5581 aactttgcga agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc
     5641 agttcaagct atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat
     5701 ggttagatcg ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag
     5761 caattgttga tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac
     5821 aaactagcgg agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa
     5881 ctatgggccg tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga
     5941 tttatcgtaa tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt
     6001 ctaaagtatt cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc
     6061 cagtaccagt ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg
     6121 tagcctcaga ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat
     6181 accattttgt tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta
     6241 aacgtaacga tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta
     6301 caaaaccaac aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc
     6361 tttgtcagcc ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga
     6421 agaaactagg aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta
     6481 ttttttttga gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca
     6541 aagagtatat tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt
     6601 cgcgtgagga cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta
     6661 atttgcgcaa attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc
     6721 taagtcttga ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa
     6781 acattgtaaa tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac
     6841 agtgggttat taaccgtcct aaagcgaatt attggaaaaa ggtttatctg gattctaatc
     6901 ggctatatcg ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt
     6961 gggcacggct ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag
     7021 acttttgttt tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg
     7081 ctggagtaac aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc
     7141 cttttgatga agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag
     7201 caattaaggc ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt
     7261 gaaggataat atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc
     7321 taaaaatgct gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag
     7381 cttagaatac ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga
     7441 gtttgaacaa aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga
     7501 atatattgca ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg
     7561 gttggttaaa gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg
     7621 tagaagatac agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata
     7681 taacagctat ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc
     7741 caatcgccaa gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca
     7801 acgttatcta tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg
     7861 aatatggtga ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc
     7921 aaaacttcct tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat
     7981 gagctacctg atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact
     8041 gagccaaaat atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa
     8101 gatttaacag gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt
     8161 cagagaatgg atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg
     8221 gtatttacgt ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct
     8281 aaaaataaat ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga
     8341 acgactgatt aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac
     8401 agaaaaaatt cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag
     8461 tcgtccactt aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt
     8521 tgagtattta aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga
     8581 atttatgaga agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca
     8641 attcgataaa ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca
     8701 acgttcgata acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca
     8761 atcgcaacga attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa
     8821 aaatgtggag cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac ggtaacaatt
     8881 actgcacatg taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt
     8941 attaaaccag gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct
     9001 gcagagatcg tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact
     9061 tcgaatacag gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa
     9121 ctagaaacaa aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca
     9181 gaacttgaag aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa
     9241 aaaacttcag ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga
     9301 aatgaacgaa gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc
     9361 taaactgaat atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc
     9421 aaaaatgact gtgggaaaag gatccatgga tatttctgtt attcaaggtg caaaggctgt
     9481 gtatattgct taccgccaag cgaacatgac tgattatatt tcattcgatg aatttatcga
     9541 taaatgggat tttgatatgg ctactgccag ctatatttat caattgatga tgttcaaaca
     9601 agcacgcgat gcttatcaaa aagaatttga aaaagcaaat aaggaaaaaa agcttcaaaa
     9661 gtaaaaatgc caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac
     9721 gttttttcta tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa
     9781 atggcttaca acaagagtgc ttatgaagga tggaaaaact atgcaataaa ccaatctcag
     9841 aaaaactaaa gaaaggaggt aaaaaatggc taaaaagaga acagaagcag aagtaacttt
     9901 catagctaac gatgacggat tgaaatctac gttaaaagaa atcagtgctg aattaactaa
     9961 aaatagagca gaattaaaac tagaacaagc tcaattacaa cagactggtt ctgaatcaga
    10021 caagttagga agtaaattat cttctttaga aaagcagtat gaattacaaa gccaaaaagt
    10081 tgaagtaact agccaacgtt tagccaatgc caaaaaatat tatggagaaa attccaccga
    10141 agttcagaaa cttgagagag aactgattaa ccaacaaaca gcgcaacaac gtttgtcaaa
    10201 cgaaattgat aaaacgagta atacactagc tcaagcaaaa ggcgaaatac agacgtacga
    10261 gtctacaatg caacagttgg atagtgaaca aaaaaatgtt caagctagtg cttctctgat
    10321 tgaatccgaa tacaaaaaat ggcaagtaac tgctggtcaa tcagcttctg aatccgagaa
    10381 attagcgaaa gcccaagaat atgtttctca acaatctgaa aatgcagaga aaacgataga
    10441 tatcctgaga cgacagttag aagctacaca gtctgagttt ggcgctacat ccacagaagc
    10501 aatgcagatg gaggcgaagc ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc
    10561 tgctaaaaat gtagatacaa ctaacttgga cgatatcgga agcaaaatag atatgaataa
    10621 tttaatggaa gcttctgacg ttttaagcga cattggcgat aagcttacag aattagggaa
    10681 acaagcagtg gactctgcta acagtgtagg tagttcccag agtaaaatac aagctaattt
    10741 tggtttgact aaacaagagg ctgaagaatt aacgaatgta gccagagaca tttattataa
    10801 aggttttgga gaatcgttag atcagtccac agatgcattg attttggtaa agcgtaattt
    10861 aggcgattta aataatcaag atttacaaaa tattacggaa caagctatgg tcctagaaaa
    10921 caccatgggc gctgatatgg atgaaacgtt acgtggtgta aatggcttaa tggtcaactt
    10981 cggattgagc gctcaagatg caatggattt aatggtttcg ggtactcaaa acggtttaga
    11041 taaaacgcac gaattaggcg acaatatggc agaatatagc caattatgga gtcaaatggg
    11101 atattcagct gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa
    11161 ccttgataaa gtcaatgact tagttaagga aatgggaata tcgttaacag atggtcgatt
    11221 tgagcaaaac atggatatgt ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa
    11281 tggtggagga acacaaaaag acgttattaa ttccatgatt caagatttta gcaatatgga
    11341 tggtcaatac gaccaattaa ataaagcttc tacaatttgg tctgcacttg gcgaagataa
    11401 cgcgatgaaa gttgtccaat ctttgactga tgttaaccat acatttgatg atgttagtgg
    11461 atctgcacaa aaaatgaatg aagattctac tactccgtta caagaattaa acggaaaaat
    11521 agctgaatta aaggattcat tagctcctat aggcaacaca atcatagatg cactcgaacc
    11581 agtaattgat tttctaggaa agatggctga tgcgtttaat aatcttccac aaccagtaca
    11641 ggattatgcc gtagcgattg gcggattgac tgctgcattt actttattaa tgccaataat
    11701 agttggcttc atggctctag gtggtcctac tacattaata ataggagcag ttattactgc
    11761 tattgctgga gttatagcaa ttataaaaaa ctggggcgca attactgact ggtttaaggg
    11821 aatatggagt aaattcactg attggttggg tggtacttgg gaaagcataa aagaaggtgc
    11881 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    11941 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    12001 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    12061 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    12121 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    12181 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    12241 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    12301 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    12361 atggacatct attagtacta cggcttcaaa tatttggaac tcaattagtg caacagcttc
    12421 tagtatttgg aatagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    12481 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    12541 ctctacagca tcaaatatct ggagtagcgt gactagtagt ataagcaatg ctataaacgc
    12601 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    12661 gaacggtatc aaaaacactg ctttgaatct ttggaatgga attacaagca ctattagctc
    12721 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    12781 ttccgacata ttcaataatg ttaaaagcac gatagctaat atatgggaag gtatcaaaaa
    12841 gactgttagc gctccgattg attggattag agataaaatc agtagtgtct ttgataattt
    12901 gaatatttct ataccacata ttccgttacc acattttaaa ttgagcggag aattcaatcc
    12961 attgaagggg aaaatcccaa cattgggtgt tgattggtat gcgaaaggta gtgtgtttaa
    13021 ttctccaaat attatcggtg taggtgaatc aggacctgaa gcagttttac ctttgaaaag
    13081 atctgtgctg caagaaattg gtgatcgtat cttgagtagc acctcagttt catctaggac
    13141 acaagcggtt caacctgtga acaattacga attcaatttc acaattgatg gtaacgcaga
    13201 tgaagtcact atgaagcaaa caactcaaca aatcattgat agcattacaa aagttcaaaa
    13261 tgataatgct tcggcatggc gttaaacagg agagtatttc tcctgttttt ttagtattaa
    13321 aaaggatgtg aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat
    13381 ttgcataaat tggctttagc tgaaagacca accatacctt ctccaaaaag acagagagtt
    13441 gaaacttctg ttttaggaag gttaggtggc ttagtgcaag attactcgtt tgaagacatg
    13501 tcgtttacat tgcactataa ctatttagag gatgtggaag accatcaagc gttcaagcaa
    13561 tcgttttata tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgacgat
    13621 cccaacgtct attacgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt
    13681 gaatggggag agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat
    13741 gtacctataa ccgtagataa accacagtca tttaacttgc tgaataatag tttagaagaa
    13801 agttttccaa agattatcat cactccttca gctacttcat gccagttcat cttaaatgat
    13861 tatgtgttta gtttcgaagg cttagtagca ggaactgatg tagtcattga tagtgatttg
    13921 atgctttgct acgaagagca atcggacgga gatattttag atcggtccaa caaaatgaag
    13981 accatgcaat atccgacatt gcaagtggat atcaatcatt ttaattgcac tggtttgagc
    14041 aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat agatgatcga taatttaata
    14101 actatttacg ataaaaatga cgcgaataat ttagctgaac atttatatga tacgcaaggt
    14161 ttaggcgctt tgtcagactg gttaacagct actgttagca ataaactaaa cggagccgag
    14221 atatttcagg gtacttatcc aataagcgga actaatgcag atttgattgt agaaggacgt
    14281 attattcagt gttatgtaga tgaaaatcga gcaaaacagc gtctacggat ttattatgca
    14341 aaaacttctg taataggcaa tacgatagaa gtaaaagctg aacctatttt caatgatata
    14401 agaaaatcgg tgttgaataa atatgacagc ggaacagaaa aaatcactgc tactcaggca
    14461 tggcaaaacg caaaagcttt agcgaaacca gctatccctt cgcagttttc tttctcgtca
    14521 ttagtagata cgcttgctaa tgtgaagata gaaaaagcga attttttaga attctttggt
    14581 ggaaaagagg gatctattct agatcgattt catggggaat ttcttaaaga taataacaca
    14641 ttacatcatg aaaaaaggct aggtacggat cataaaatca aagcgattta tactaaaaat
    14701 ttaactggtc ttgacttaga gatagatgct caaagcgttt tagttggagt ttatccattc
    14761 attagcagtt cttcagaagg agaagacgag atcactctac cagaagaagt cattttcacg
    14821 gattacgtgg atgattatcc tgctggatat gtttcttttg ttgattttaa agacaaagcg
    14881 actgatgtag ccacattaag ggaagctgct aaagactggt tgaaaacaaa catagataaa
    14941 caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca aagaggctat
    15001 gaaaaatttg ttgatctaga aaaagtttcg atgggtgacg gagtagatgt gtatcatcca
    15061 cagttaaaag tgaatatgtc agcgagaatt gtggaatata cgtttaatgt tctaaccaat
    15121 tcatacgata aattagttgt aggaaacgtc aaaacaaact tcttagaaaa cacagagaat
    15181 aatgtaagca atttgattaa tgatgccatt gatcaattga aaaatggtgg cgaaatcagt
    15241 gatttaatca atgatattgt agatcatcaa actgatatga ttactggtca aaatggtggt
    15301 tatgttttat tagatcctaa agaagcgcct agtcgtattt tgattatgga cacaccagat
    15361 aagaataccg caaggaatgt tttacaaatc aacaatgctg gtattggttt ttctaaaact
    15421 ggcattaatg gaacatatga aaccgcatgg acattagatg gcggattcaa tgcctcgttt
    15481 attacggctg gtgaaatagt aggaattact attagaggta ctacattaat tagtgatggc
    15541 actgattata gaacaagtat tgctaatggc aaaatgactt ggtactcaaa aaaagttaac
    15601 aaagatatta tggagctaga agcacgtgat tatgtaagtg ctgatgccgg tattgtatca
    15661 tacaccatga aaactggtgg tggtttcatg attagaaatc cacagggtaa cttggttttt
    15721 agtacgtggg ataatggtaa taacagaccg tttctatctt ttggtgcgcc caatttcagg
    15781 tatagcaatg ctagttatgt aacttctggc gacggtagtt ctttaagcat taatggtagt
    15841 gcgggtaact catgggaatt taaggtagct ggtaggacta tgaaatttac tagtgatggt
    15901 atgctaacgt tgccaggttg tttttttggt tcatgggaag atgggaaact tgctaggttt
    15961 gaacaatcaa cggtacaagt atataaagat tttactgtta gaggtactaa aaactcaacc
    16021 gtaccaacag aacattatgg acaacgacta ttaaacgctt atgaaactcc agaatattat
    16081 ttcgctgatt atggggaagc cgttacaggt gatgatggta aagttcgtgt tgatattgac
    16141 cccatgtttg ctgagacagt aaatctaagt cgatatatga cacatgtgac acctacagaa
    16201 ctagttttgt gtgctgttac tcatgaagat attgaccatt tcatcattga aactagtaag
    16261 ccaaacgtat tagttagatg gaatttagtg gcacaccgtc tagggtatga agatattaga
    16321 ttaaaagagg atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa
    16381 gacaaggagg tatataaatg gctagcagtt tatataattt ggctctagat ttcagcaaag
    16441 aattaaacta caccaaagct attatggctc gtcaaggtga taaagggatt acagtgactg
    16501 ttaaaccatt tttaaatggc ttgcagatgg atacgagtgg cggaacattt actttaaaag
    16561 gaacaacgcc atctaaccgt tacgtagata gtgtggcaac tagtgtaact agtgaagaag
    16621 tcacgttttc tcttgatggc acatttatga gtgaagcagg atattataaa cactgctacg
    16681 tagaatatag aaaagacaat caaattttaa caacgcaaga tatcattttt ttctcactag
    16741 gagtgtctga catttcgcaa ggccaagccg atgaatacgt ttcgcaattg gaagagttga
    16801 ttcaaaagta taacgaaact tttgatgctt ttatggctga aatcgaaggt agagtggaca
    16861 gcttaaataa acagattact gatttaactg gtcaagctaa aacactacaa gacaagttag
    16921 acgctctgaa agaagaaatt tctaaattag gtaacttgca ggtgatgtac agtaacagca
    16981 tcga
//
LOCUS       DACDBG010000054        16910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDBG010000054 DACDBG010000000
VERSION     DACDBG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.054"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IVZ90_001841"
     CDS             complement(<1..456)
                     /locus_tag="IVZ90_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8262884.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IVZ90_001842"
     CDS             complement(561..1424)
                     /locus_tag="IVZ90_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8262885.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IVZ90_001843"
     CDS             complement(1620..3719)
                     /locus_tag="IVZ90_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8262886.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IVZ90_001844"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IVZ90_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8262887.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IVZ90_001845"
     CDS             complement(5072..6913)
                     /locus_tag="IVZ90_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8262888.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IVZ90_001846"
     CDS             complement(7095..9488)
                     /locus_tag="IVZ90_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8262889.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IVZ90_001847"
     CDS             complement(9595..10455)
                     /locus_tag="IVZ90_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8262890.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IVZ90_001848"
     CDS             complement(10514..11035)
                     /locus_tag="IVZ90_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8262891.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IVZ90_001849"
     CDS             11355..12074
                     /locus_tag="IVZ90_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8262892.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IVZ90_001850"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IVZ90_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8262893.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IVZ90_001851"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IVZ90_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8262894.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IVZ90_001852"
     CDS             16434..16790
                     /locus_tag="IVZ90_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8262895.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4644 a         3506 c         2971 g         5789 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa
//
LOCUS       DACDBG010000055        16901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDBG010000055 DACDBG010000000
VERSION     DACDBG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.055"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(29..727)
                     /locus_tag="IVZ90_001853"
     CDS             complement(29..727)
                     /locus_tag="IVZ90_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8262896.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="IVZ90_001854"
     CDS             complement(720..2072)
                     /locus_tag="IVZ90_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8262897.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="IVZ90_001855"
     CDS             complement(2295..3695)
                     /locus_tag="IVZ90_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8262898.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="IVZ90_001856"
     CDS             3856..4593
                     /locus_tag="IVZ90_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8262899.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="IVZ90_001857"
     CDS             4718..5197
                     /locus_tag="IVZ90_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8262900.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="IVZ90_001858"
     CDS             5194..5961
                     /locus_tag="IVZ90_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262901.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="IVZ90_001859"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="IVZ90_001859"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8262902.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="IVZ90_001860"
     CDS             complement(6864..7466)
                     /locus_tag="IVZ90_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8262903.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="IVZ90_001861"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="IVZ90_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="IVZ90_001862"
     CDS             8147..8731
                     /locus_tag="IVZ90_001862"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8262904.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="IVZ90_001863"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="IVZ90_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8262905.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="IVZ90_001864"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="IVZ90_001864"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8262906.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="IVZ90_001865"
     CDS             10632..10811
                     /locus_tag="IVZ90_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262907.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="IVZ90_001866"
     CDS             10833..11858
                     /locus_tag="IVZ90_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8262908.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="IVZ90_001867"
     CDS             12033..13277
                     /locus_tag="IVZ90_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8262909.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="IVZ90_001868"
     CDS             13430..14377
                     /locus_tag="IVZ90_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8262910.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="IVZ90_001869"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="IVZ90_001869"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8262911.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="IVZ90_001870"
     CDS             complement(15279..15521)
                     /locus_tag="IVZ90_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262912.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="IVZ90_001871"
     tRNA            15642..15714
                     /locus_tag="IVZ90_001871"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16472)
                     /locus_tag="IVZ90_001872"
                     /pseudo
     CDS             complement(15833..16472)
                     /locus_tag="IVZ90_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(16595..>16901)
                     /locus_tag="IVZ90_001873"
     CDS             complement(16595..>16901)
                     /locus_tag="IVZ90_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8262913.1"
                     /translation="TLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFS
                     TNDLENASFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5761 a         3001 c         3377 g         4762 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt tcgtatcttt tttaccaaaa
    16321 acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat aatggatggc
    16381 gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat ccctttttta
    16441 agttctattt tttttcctaa aatgttattc atgataataa ctcctactat tcagtttttg
    16501 aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat ggatatttgt
    16561 attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt tgtaaaaatt
    16621 ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga tgttccgagc
    16681 gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa tcagtatcaa
    16741 aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg gaacagtcta
    16801 caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg tttgaaccag
    16861 ataaatttac atagtccatt ttacaattct cgaataacgt g
//
LOCUS       DACDBG010000056        16796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDBG010000056 DACDBG010000000
VERSION     DACDBG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.056"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..315
                     /locus_tag="IVZ90_001874"
     CDS             <1..315
                     /locus_tag="IVZ90_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262914.1"
                     /translation="LRTQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFFGDDFDFET
                     NQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEYKKIANFESLYKLFNET
                     KL"
     gene            359..1531
                     /locus_tag="IVZ90_001875"
     CDS             359..1531
                     /locus_tag="IVZ90_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8262915.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1518..2222
                     /locus_tag="IVZ90_001876"
     CDS             1518..2222
                     /locus_tag="IVZ90_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8262916.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2227..3387
                     /locus_tag="IVZ90_001877"
     CDS             2227..3387
                     /locus_tag="IVZ90_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8262917.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3463..3741
                     /locus_tag="IVZ90_001878"
     CDS             3463..3741
                     /locus_tag="IVZ90_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8262918.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3722..4093
                     /locus_tag="IVZ90_001879"
     CDS             3722..4093
                     /locus_tag="IVZ90_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8262919.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4109..4513
                     /locus_tag="IVZ90_001880"
     CDS             4109..4513
                     /locus_tag="IVZ90_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262920.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4510..4920
                     /locus_tag="IVZ90_001881"
     CDS             4510..4920
                     /locus_tag="IVZ90_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262921.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4981..5568
                     /locus_tag="IVZ90_001882"
     CDS             4981..5568
                     /locus_tag="IVZ90_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8262922.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5641..6108
                     /locus_tag="IVZ90_001883"
     CDS             5641..6108
                     /locus_tag="IVZ90_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8262923.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6154..6462
                     /locus_tag="IVZ90_001884"
     CDS             6154..6462
                     /locus_tag="IVZ90_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262924.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6657..13391
                     /locus_tag="IVZ90_001885"
     CDS             6657..13391
                     /locus_tag="IVZ90_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8262925.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13395..14267
                     /locus_tag="IVZ90_001886"
     CDS             13395..14267
                     /locus_tag="IVZ90_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262926.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14264..14891
                     /locus_tag="IVZ90_001887"
                     /pseudo
     CDS             14264..14891
                     /locus_tag="IVZ90_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14852..15313
                     /locus_tag="IVZ90_001888"
     CDS             14852..15313
                     /locus_tag="IVZ90_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262927.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            15317..16141
                     /locus_tag="IVZ90_001889"
     CDS             15317..16141
                     /locus_tag="IVZ90_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262928.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16159..16704
                     /locus_tag="IVZ90_001890"
                     /pseudo
     CDS             16159..16704
                     /locus_tag="IVZ90_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         6006 a         2577 c         3572 g         4641 t
ORIGIN      
        1 ttaagaacac aaatttctcc agctgaattc caagtgattt tattcaattc tctatatata
       61 aaacgaggtt ttggcttagg aattcaactt attggaagtg gtttttttgg agatgatttt
      121 gattttgaaa ctaatcaaca ttttgaaaca agtatcaatg aacagtggtt tctatcacta
      181 tcaactgttg acagtgataa ctctataaag cgacaaaaat tatctgaata tattaaagaa
      241 ttaggatccg tagaatataa aaaaatagct aattttgaaa gtttatataa actctttaat
      301 gaaactaagc tctagaaata gggctttttt atttttcttt gaaaggtggt gaaaacatgt
      361 gagcttattt gatatcttta agcagtcaat aagaaatgaa gagccttctg agtggatacc
      421 ggattttgta tatggagagg aagtatctgc tcgggcatac ctcaaaatta tggcaaaaaa
      481 tactgtttta gattttgttg caagaactat gtcaacgtta gaagttaagt ttaaaaataa
      541 agatgggaca tcagactggg attatattct aaatgttcga cctaattctg atatgtcagc
      601 agctacattt tggcaaaaat ttttttatcg gctcttagat gacaacgaag tgttagtaat
      661 atttacagat gataatcaat tgctgattgc tgatgatttt agtcgaacgg agtatgccgt
      721 gtatgatgat gtttttacaa atgtgatggt aaaaaatttt gtctttgaac gttcatggaa
      781 catgtctgac gtcatttata tggaatataa taacgaagaa ttagaaaggt ttacacgagg
      841 tttatttgaa gattacgccg cgttgttcgg acggattcta gaagttgcaa tgcgtaacaa
      901 ccaaattcgt gggtctgtat ccattgattc aacgggaacg gcgaatgatg aaaaaggaaa
      961 agatggtaag actagatctc aaagattaca agaatatatt gataggattt ggaactcttt
     1021 taaaactaac tcagttgcaa ttgttcccaa agtgaaaggc tttgagtatg aggaatacac
     1081 caataaacaa ggcgtatcga atcaatcgct agatgaactg aataagatga agtcgtcttt
     1141 aattgatgat gttgccaacg ccataggagt acctacggcg cttatttatg gtgaaaaagc
     1201 tgaactggat tcaaatatta aagcttttcg taaattgtgt attgctccac tagttaaaaa
     1261 acttcaagac gaattggtgg caaaaataat tagtcgtcaa gagtacaaaa acggggaacg
     1321 tatagaagtt aaaaaagttc tccaaaaaga tctattagag tatgctactc aaattgataa
     1381 aataacatct ggtggtgcgt tttatattga cgaagttcgt gatgaagttg attatgatga
     1441 gttgccaaat aatgaaggaa aaattcgtat tcgtacgaaa aactatgaag aaactgtgaa
     1501 gggaggtgag aacgacaatg ccgaaagcta aaaaagtacc atttcagttt actaacgaaa
     1561 ttcagaatgg taagcacatt ctcaccttga gtggaaatgt acagaaaaaa tattggcgtg
     1621 atgatgatgt cataaacgct aaagatattc gtgactctct agatactgta accgatgata
     1681 ttgtaatcaa gttgaacagc ccaggcggtg atgtgtttga agggattgag atttacaatt
     1741 atttaaaaga tcacccatca aatattactg tagaagttac agggcttgcc gcaagcgcag
     1801 caacgtttat tgtcgctggt gccaatgatg tgattatgaa tgttgggact tctttgatga
     1861 ttcatgaagc ttctacattt gcttggggga ataaacagga catccaaaaa acgttaaacg
     1921 cattggaaac gatcgatgat tctatcttag ctatttattc agataagact ggccaatctg
     1981 ttgataagtt gcgtgaatgg atgaatgaag aaaaatggtt tacagcagat gaagctgtca
     2041 aatatggatt tgccaattct gtgaagcgtg aagaaccaca aaatgaacct caaaatattg
     2101 cagcgatgat tcaagatgct gttgccgttg caatagcgaa tttaaaggaa cctgttacta
     2161 ataaaatcga acaagaatca aaacaaaaat cattaatcgc acgattgcga aaaggagaat
     2221 aaagatatgt taaaaattac agataaaact gcagatgcaa aaaaggcatt taatgctatt
     2281 tcttcgaaag aagatgcaac acccgaacaa attaatgctg ctctagaaga atatgtaacc
     2341 gccattgcag aagatgccgg caaacaggtg cgtgctgaat atgaagaatt gaaaaatgtg
     2401 acggacaacc gtgtattaga ggctcgtgga attcctacac taacagcaga agaaacaaag
     2461 ttttataatg aagccgtaaa atctggaggt ttcgattctg acgtagtttg gccagaaacg
     2521 attttggaac gtgtttttga aaatcttcaa aacgatcatc ctattttagg cctcattaac
     2581 tttacaccaa ctgtaggtcg tgtcaaagta attagagcac gtcgtaaagg tgttgctgtt
     2641 tttggaccat tgcataaaga ccttgaaggc caactagatg ctgaatttgg tgcaactgaa
     2701 tttgttcaac ttgcgttgac cgcatttttc ttaatttcta atgatacctt agatttagga
     2761 ccacgatgga ttgaccgttt tatcaatctt tctttaagtg aagcagtacg tgatgtttgg
     2821 gctaaaaaaa ttattacagg ttctggtaaa gatgagccaa ttggattgct taaagatcta
     2881 gatggtgcgg tcacaagtgg tgtatatcca gataaagcta gcgcagggac attaactttt
     2941 gcgaaagaga agattgttct tgaacttgca ggggtgatga aaaaattatc taaatacaca
     3001 tacaaagtag atggtgacga tgaaggtgaa gtgaaatacc gttctgttgc tggacgagtg
     3061 tatctaattg ttaatcctgt tgattactgg gatatcgtag ctagagttac atttgcaaat
     3121 ttaaacaatg tgtatgggac tacgatgcca tttatcgctg ttgatcatat catcgaatct
     3181 gtagacgtac cagaaagtaa atgtattgca tacgttgata aagagtatga agctactcaa
     3241 tcaagagctg aaaaagtcta tgtatataaa gaaacttttg caatgaaacg cgcaactttg
     3301 tatgctgtcg acatgcttgg taatggctat ccaacaaata atgatgcagc acaagtatat
     3361 gacttggact ttacagcggg ggagtaatgt cccctgcaat tggtgagata acaccgacga
     3421 ctgatggggc agtcattaat ctttcgtaaa tggggttaga gtatgaacac tgaagaattt
     3481 ataaaagaat ataaggcccg atttcgtatt ttccatactt ccgaagatga ggatatcggg
     3541 aaacagttag agagcgggtt tgccgatata aaatctctaa tcggcagttt tgatccttcc
     3601 acttatgaga aaggaaaaga attagtttat gaacgtactc ggtatctacg gaacgaatca
     3661 ctagagtact tttacgacaa ttttcaaaca atgattatgg atgcatcaat tgatctggca
     3721 ggtgctggat atgccaatta aatcaaatta taagaagcct aaaattgtag ctggtgatct
     3781 aaatacgcca gttacttttt ttgagtttca acctaataag ggtccagaac ctggtgatga
     3841 agaaaaaaag gaactgtatt tttgtacagc actagtttac aacccatcaa tgaaagatcg
     3901 tgacattctt ggcacaaaag aaggcgttac gataaaaata cgggatcccc atcaatctta
     3961 tattcctact aacaaacata aagtaaggat attagattat cgatatggag ataaagtttg
     4021 ggaaataatc gatgtctcac cggatttgga agaaaattca attgtaaaga tagtattaga
     4081 attaattact taaatttggt gataaatgat gagtaacgta aagtttgaag gcgttcaaga
     4141 aacaatccaa gctttagaaa aaaagtatgg agaaaagaaa actaagacat taacaaaaaa
     4201 agcaatcaat gttggcgctg aaaaagtaga aaaacagttg caaacggaca tgaccgtttt
     4261 taaagacaaa ggttatacga tcgctgaggt tgttcgcaag aacgctacgt atagagatta
     4321 caaggcagaa gctgaaatcg gttggaatgg tccacatcaa cgatatagaa tcattcattt
     4381 gaatgaatgg ggatatacac gaaagggcaa acaaattaga ccgagaggat ttggtgtaat
     4441 aacaaaatct cttaagaatt ccgagctgtt gtactttgat gctgtaggag aggaggttaa
     4501 aaagagttta tgaaagatat gcttaatatc atttacgagg ctctttgttt gaatgagtat
     4561 attcattcaa tgacctacaa cgagaatacc gagcaatatc gtattaaatt ttatgagcaa
     4621 cctgaaaccg ccgataaaac aggtccaatg atcacaattc gtcctgtaga tacacctaac
     4681 gaagcgtacc acggaagtaa caaagagctt tctgtggagc atatgataca gattgacgtt
     4741 gaatcgaaat atagagcaac atgcaagcag attcaatatg agataaaaaa agagatgaaa
     4801 aaactaggct ttggccaaat ttccggtcaa gggttagatg aatactttac tgaaacaaac
     4861 cgctttgtag acgcgcgtcg ttacgaagga aatacaaaaa tttacgatac cgaatattag
     4921 aaattaagac acgaaaataa cgtgtctttt tttattaccc aaaattagga ggaaaatttt
     4981 atgactttag taggattcaa aaaaatgaca atcggggtct ttgacagcac agggaagatt
     5041 ccagcagcaa atttatatgt aattgaaggt gaacaaaaca aaggggcgac tgtatctgct
     5101 gaaatcagtg ggctatcaaa agaaccatca aaagtttacg gatcgaacat cgcttactat
     5161 gtctctcaaa aaggaacagg cgatgtttca gctacttttg gattgttaga tttaccaaca
     5221 gaagtaaatg acaaaattct tggttacaaa accgatacaa acaaaatcag cttcttggga
     5281 gaagatactg agccaccata ttgtgcaatt ttgatggaat cggaggattt gaacggcgat
     5341 acagcaatgc ttgcgatgtt caaaggaaaa ttcagtcgtg aatctattaa tcttaacact
     5401 acgacaaatg aagctttcga accagaagca gaagaatatg tattttcagc gattgccaat
     5461 gatgttgaag gtgatgctaa aggccaaaca gtcgcaaaat atgttggaga cgaagaagct
     5521 tctattactg gtttgaaaga gatggtattt cctgcagcgg gggagtaact agccccgttg
     5581 ttggagcaat cactcccact attaatgggg caacagttga attaagttag gaggataact
     5641 atgccagata catttaaaat ctataaaaaa aatggtactg aatttacaaa agtcgcagag
     5701 ggtgaaagtc cactttctat tactggaatt gcagcaaata ctcaagttgc taaaggagat
     5761 tatcaagcta cgcgtgtggt cggaggtcaa gaatcagtaa aagtagatat tcctgcattc
     5821 accactcttc caatttcagt tactggtgtg acgttggata aaacgacagc tgaagttgaa
     5881 caaggcggaa ccttaaaatt aactcctact gtaacaccag caaatgcaac agacaaaaca
     5941 ggaagctgga gcagttcgaa tacagctgta gctacggtta ctggaggcaa cgttacagta
     6001 aaaacagacg ctgaagttgg cggaactaca gaagtttcat ttactactac ggatggcggt
     6061 aaagtagcaa aatgtactat tacagttatt gaaaaagcag cgggctagtc ttcggactag
     6121 cttttttatt tggaaaataa tggaggaatc taaatggcaa aagtaagaat tgaattgaaa
     6181 gaagaatcag gaaagaaagt ctatgaaaac atagacacaa cagggaaaga ttatcgaaaa
     6241 gctttagaaa caatcaaaaa gctaaatgaa gacggagtaa tgatttggaa tcgtttagac
     6301 atttatttgg attttgctgt aggtatcttc agggatagca aattgacagc tgaacaaatt
     6361 cttgatggct taccatctga gaaggtaatg agcacattgg acgacatcct aggagaagta
     6421 atggggattg aaagtaatcc agacccagac gcaaaaaagt aacacccgaa gaagcagaag
     6481 aaatgtactt aaacctatgc agagagttag tgaaagcggg atggtcacta tctgacatag
     6541 aagataattc ctttgaaatg atgatgaaaa ttgcgtgcac ttcgcctaag aaagagaaac
     6601 aaaaagaagt tgatcttaaa gacttcttga aatccatgta gagaggaggt aaacatatgg
     6661 caaatggaaa acctttaggc aatatgatta tcaaactaga cttagacagt tcagcttttt
     6721 caaaaggtct agccggagct aaaaacgctg ttaaccatca aatgaaagca atgaaatcac
     6781 agatgcaagt aatgaactct tcaggaaatt ctctcggtgc tttacaagca aaatacaatg
     6841 gtttaggcgg agttctacaa gcaaatgaaa agcaagtaga attgctcact aaggcttata
     6901 aggatagttt tgactctaac ggaaacgcta ctgcttcgac tgctaaatac gctaatcaat
     6961 tgaatcaagc aacagctaga tcagcaagtt acgaatctca gatgaaaaca actgttggac
     7021 agattgctcg gatgaaagtg gaaacggaag ggtttacggg gaaactaaag gctaattctg
     7081 atgtattgat taattctggg aaaaaaattg aagcctttgg taaaggagtt tctgggatag
     7141 gatctgccct aactgttgga gtaacagctc caatagtggc aggcgcaact gctgttacta
     7201 gtgcagctat ttcatgggaa agtgcttttg caggtgtaaa aaagacaaat gatgaagttg
     7261 tagactcgaa tggaaacgta gtttattcct atagcgactt agagaaaggt ctgcgtaatc
     7321 ttgccaccca attacctggt agccatcaag aaatagcaaa tgtagccgaa gcagccgggc
     7381 aattaggtat aaagactgaa aacgtagtta gcttcactaa aacgatgata gacatgggtg
     7441 aatcgactaa tatgtcggct gaaactgcgg caacttcttt agcacgattt gctaatatta
     7501 cccaaatgtc tcaaaaagac tttgataaat taggatcagt cattgttgat ctaggtaaca
     7561 attttgctac aaccgaatca gaaatcactg aaatgggatt gcggttagcg ggagctggta
     7621 aacagattgg aatgagccaa ggcgaaattc taggatttgc tactgctcta tcttctgttg
     7681 gtgtcgaagc tgaagcgggt gggtctgcct tttcgaaggt catgatccag atgcaactag
     7741 ctgtagaaaa aggatttggc gcttttgatc agctgaaaca gatggcagaa gaacaaggtg
     7801 ttccgtgggt taatttagta aatgctgtga gagacggtgg aaaatcgtta aaagcagtat
     7861 cagagcaaat gggattcact tcatctgatt taaagaaaat gtataaagaa gcggacaatt
     7921 cgaaaagttc gcttgaaaat tttgcgaatg tggcaggtgt tacaagtgaa caattccaga
     7981 agttgttcaa gagtgatcct tcagaagcaa tcatcaagtt cattcaaggg ctgaaagatt
     8041 cagaaaaaca tggtacttca gcaatcaaag ttctggatga catggatata aaagaagttc
     8101 gactacgcga cagtttattg agagctgcaa atgctagtga catattcaaa ggagcaattg
     8161 aaaaaggaaa cggcgcgtgg aaacaaaata cagctttaac aaatgaagct aataaaagat
     8221 acgaaacaac tgaatcaaag ctaaagatat tgaaaaacga agtgattgat acagcaatcg
     8281 atcttggagg tccatttgta gacgcgttgc gtgatggtct aaatgtatcg aaaccattga
     8341 taaaaaactt agggaatatg gcgaaagctt tctcagctat gagtgatgat caacaaagaa
     8401 acattatcaa atgggttgga ttagcagcag cagctggtcc agcgctgaag cttttaggaa
     8461 ctggtacttc agtaataggt aaagttactt caactactgg aagattgacg aaaagccttg
     8521 ttgatttagc agcgaaagca gcagaaaaga aggctatgga tgcctttgca tcgtcggtaa
     8581 caacagctgg tgctgcagct gcaaagacag caggagctgg aggtattggc gggttagtcc
     8641 ctatgatggg aaaaacagct agtgctgcaa gtactgctgc gggcgccagc ggaataggcg
     8701 caatgacaac gtcactaggt ttgttaggtc ctgctctact tggtattgtt ggcgttggtg
     8761 gcgcactggc agttggctat ggagcatgga aattatttgg tgaagaagct tggaattcct
     8821 cgcaaagagt aaagcaatgg ggaacagatg tcggtgagga gatagatagt actcttgacg
     8881 gagtacagaa gaatattgaa ggtgcaaatg gtcaattcgg tcttctcaaa gagggtttta
     8941 ctgttgatga tgcaaataat atggcggcta atttcgcaac cgctggaaca tctttggaaa
     9001 catctttaac gggtagaata aaggcattag acgatgcact aaaagggcta cctcaatcag
     9061 ttcaggatgc catgaaagaa gtagctgaat cagaagaaga aaccttaggg caatctcttt
     9121 ctaaaataaa agacaacaat gaagaaataa aaaaaatcag agaaagagcc gcaaatcaga
     9181 acagagactt gactgtttca gaactacaac agatacagga tctaatgaaa ggaacttctc
     9241 aagcgtatgt ggatacgctc gatgtttctg ctaaagacag aaaaaagata ctatcagcca
     9301 tgaatggtga tgttgcacaa gctacagaag aagaagcgaa gacgtggtta caatctttag
     9361 cgaaacaaaa agctgcaact accgaaaatt atagacaaat gcttgaagaa aaaaaggcgg
     9421 ctttaaagga aaagggagta agcaaagaat tcattgatgc attagaagat gaaatgaatg
     9481 attacatcaa aactacaaat caaggattcg atagccaaat agctgctata acaaataaat
     9541 atccacaatt gattaacgaa gtgagtttat caaatggtca attatttagc aaaacggcag
     9601 ctgctatgga caatactggt cagtatacaa aagtttttct cgaaagtaat gaaaagattt
     9661 tagcccaagc gaagaaaaca tcggatgaaa tagcagataa tgccgaaaaa aatgcagaac
     9721 aacttggtat ggtagctgat gaggctacgc atgctggatc aacttggaat ggcttagttt
     9781 tagacgaaaa aacaggaaaa gtaaaaacta atgtacaaga agtagtgaat gaagcaacga
     9841 aggatttcac taaatggaat gaattgaaac ctgttgttca tgatgcgaaa ctaacaacga
     9901 atgcaaaaga tgtcatttcc atagcggcag tacaaaatgg ctattgggat gatatggaat
     9961 ggaaagaaaa agaagcatta ttagaagatg agtgttctaa aaatgtatac aaggctttag
    10021 aaaatgctgg taaatggaat gagttagata ttccttctaa agaagcaatc ctaacttcaa
    10081 atactcctga agttatgggt gaaactctca tgaatttggg attatgggat acttacaaac
    10141 ttcaagtgaa agatttagat ttgaatgcgt ataaagtata cgatgctatc gatggatcag
    10201 aagaaaaatt aaatagttgg gctcaattag ataaccccac aaaagaattg ttattagata
    10261 actcagattt ttctacaaaa ctttttaatt ccgaagaaat gtttaaacgt tggaatcagt
    10321 tatcatctaa tgcaaagttt ctttcggcta ataatactaa atttacagag gttcttttat
    10381 catctgacag aatgtggaat caatgggtaa accttcctga taatgagaaa agattacttg
    10441 cagataacga agatttagct accactgttt tatcttcaga agaaatatac ggaagatgga
    10501 ctcaattacc agataatcaa aaatatatgt tagctgacaa tactgactta gcaaacaaaa
    10561 tattcgcttc agaagaatca tggaatgcat ggaaagcaat tccagacaca gttaaaaact
    10621 tgcttggtaa taatattgat ttactggcta aagtgaaaga cgggactgta agtgtagaag
    10681 aatataacaa agttatgcca cttcttaaaa ctctatatgg agataacgtt aatttaaaag
    10741 aggcaatcag tggggctaaa agctctatag agaattacaa caaaaatatt tttcctagtg
    10801 ataaaactgc agtaggacac gatgaggctt cacaagctgc taaagatgca tttgaagcgt
    10861 tcaacatctt ccaaacaaaa ataccagata aaattactaa aactgtatca gctgacttta
    10921 taggtcctat gccgaaaaat gcgaaaggaa caaacttcca tcctggcgga gcagctatgg
    10981 taaacgatca aaaagggcct acttatgaag agcttataac cttgccaagc ggagaaactt
    11041 ttatacctaa aggacgtaat gtcgttttag acttaccaag aggttcgaaa gtactgaacg
    11101 ccactaaaac taaacgtcta gtgcctaaat atgctgatgg tataggaaac ataacgacgg
    11161 tttcatctac gctaaactta gatgctttaa ttttagctat taacgagcta acgacggtat
    11221 tgagaactca actgatggca ggaacgactg atgctaaaga tactaaacta tcagatgaaa
    11281 caaaagttgc cgatccgatt attccggaca gtctttctga aaaatcagat cagtatctag
    11341 gaattggagc tcaatggtta actaatctaa tgaatggttg gaactcagcg gttcctcaat
    11401 atatgaatag tgaaatggtt tttattacaa actatctcaa tgcgctaaaa cttcaaaaca
    11461 atcctaatta tacgcaagga gcaacatgga ataagaactt actgaatgga tggaatagtt
    11521 taactgggac ctttattgct acaatcaatt cattttgtaa tcaagcaatg gtcacgctta
    11581 gaaactacaa cactcctatg tacaacaatg gccgaacgtg gcagcaaaat aatcttaatg
    11641 gttggaattc attgtatgga tcattcatag cccgtgtaaa tcagcttggt aacgattcga
    11701 ttaacaacct tcgttctaaa agtggtggtt tttacaatgc agggacattc ttgttgcaat
    11761 ctttaataaa cggaatgaat tcgttaggta attctctttc tactacaatg aacaatgtcg
    11821 ctaatacaat ggttggtggt ataggcaaag gagttaatgg cgtcatatcg ggagttaact
    11881 atgtgcttaa ggaagtcgaa tcaagtaaga atatcggaaa ctggccaatt ccacaatacg
    11941 ctaagggaac tgatggacat cctggtggac ttgctatgat taatgatcaa aaaggcctag
    12001 ttcacgaaga atatgttcag atgccagatg gacgtagttt tattgccaaa ggtagagatc
    12061 ttcttgttaa tttgcctaaa ggtgctcagg tcttaaatgc ttctttaaca aagaaattaa
    12121 aggaacgttt aaatattcca cactacgaaa aaggtattgg caatttagat attgttgatt
    12181 tgttagatga tgaaaaaaga atgttagaat tcttaactag caaagttgat ttttcaggca
    12241 ttaatgaacc atggcttgat atgactaaat caggaacgtc attaatgtcc aaagctgcaa
    12301 atacaatgct tcaatcaaaa ttaagtgaat tcttcactca tggcaatttt gatggtgcag
    12361 taaatgccaa tggtgtttat caatatttag ttgatgtggc acagaaagta atgggtaagt
    12421 tccccggact tacagtaact tctggatatc gagcaggaga tgcctactat cacggaaaac
    12481 ggcaagctat cgacttggct tatccaggta tttctggaga tccgagatat acagcagcag
    12541 caaattacgc ttttgaaaaa ttcccttcaa aaattgcgta tgtcattacg aatggacgtg
    12601 tacgtgaccg catgggatta tctggaacaa ggtcaagcgg acaatggacg aattggccag
    12661 atggcgatca cttcgatcat attcatttaa acggttcgat ggggtctggc gatatattca
    12721 atggaggagt tggaggaagt ggtgttgcac gctggcgttc ttatgtgtct aaagcattga
    12781 aaatgaatgg attaccagct acagcagcgt atataaatgc ctggatgtcc caaatccaaa
    12841 cggaatctgg tggtaatgag aaagcaatcg gcggcaatga tgggctcgcc gaaggcaatg
    12901 caacaggatt acttcaaacg aaacccggaa cttttgcagc taacgctttt cctggtcacg
    12961 gaaatattat gaacggattt gataacatgt tagcagcaat caattatgct aagaaacgtt
    13021 acggagttgc aggtatgttg caagttatcg ggaaaggaca tggatatgcc aatggcggct
    13081 tgattaccaa agacggacta tatcgagcag gagaaggaaa taaaccagaa atggttattc
    13141 cattgacaag aaaaacaaga gctatcgaac ttatgggtca agcattagcc ttcctttctg
    13201 gaagcaataa aaaagaaacg aaaagtgaga ttaattctgt agataataga catatcctac
    13261 aagcgataga aaaacagtct cgtatgacaa atgatctgct atcattatta ctctcgtttt
    13321 ttaaaagtaa caatcgatca gataaagaac tagctttaga tattcagaaa attttaatca
    13381 ggaggacata gtatttgagg acagtaatat taagaaataa attgaatgaa gaaattgatt
    13441 tgtccacaga agattatttt gctactgaaa tgtcgaatct aggttttgaa gttacaaaag
    13501 agcatattgg acaatggggg agtttcagag aaataggaga gagtattgaa ttgtctgaat
    13561 ttcaatcatc agttatgatt tctgttcacg gatttagaga acaacaatta tatggttcat
    13621 tagttgaatt tctggctaat ggaccttttg aactagaatt ttcttttgat tcagaaacaa
    13681 tgatcaggaa atgtagtttg aaatcattat ctaaaacaga aattgattcc caaagctcat
    13741 tgctaacaga tactttgaat ctatatttta cttcaagctg gtattctatt aagcgtgaaa
    13801 aattaataca gaattcaact tctctcaata cacgtggaaa agtttactca tttgttcgac
    13861 catatgtcta caccaaaaat gtatgggaaa agaaaggtgt atttaagttc agtaataatt
    13921 ctgtatattt gacaaatagt acagagcgaa tgtctccgtt aaaaataagg attattggag
    13981 agtgctcaaa tccatattgg gaagttatcc aaaactctaa agtagtagct tcagatggct
    14041 attttattaa tatgaaagac acacaaatat tagaagtgtc tagtttgcta gaagaccaaa
    14101 cggcaatttt aaaagatgta ggtggagttg aatcttctgt ttatcaacaa caagatttta
    14161 ctaaatcaaa cttcgtccaa gcgcctctag gagaattcag cgttgttttt catgttggac
    14221 atgcaaatgt agagattgaa ctttatgagg agcgtgatct attttgattt tagccattac
    14281 cctctttcac cgagatatga atttatataa cgaacgttta atttcgactg agttcgaatt
    14341 tggaacggat gaaattggag aagaagtaag taattttatt acagataaat atgtccatgt
    14401 aaaatcagga gattttctac tagcaaaatt tattcctagc gggaagattg cttattttgg
    14461 agtgattagc tcgcaagaag acaaaaaaat aaattgcaaa ggattactca gtttggccga
    14521 tagcgagata ccgactgtaa aaatatcagg tgctaattat gaagagcata ttcgacgact
    14581 gattgaatac tacttattga acgatccaac aaaacaacta aaaaatattt tggaggtaaa
    14641 aacagaaagc gttacttctc attcttatca agctgttgac acaaataaga gacgattgaa
    14701 tgcatatata ataaatgggt tcaaaaaata taatgttaaa tggtatgtca aagagattaa
    14761 aggtaaaaaa atatatactg gtattcgagc agttgatgat tcaatttata ttaaagataa
    14821 ttcatctgag tttaatgact gggatgtttt tgtgcaatct cctggaccag ggaacgagaa
    14881 caaattattg atcgtagata aagcaatgaa agatatagaa aatcccttgg tattgtctac
    14941 gtggtattta gatgacgaaa ataatcttac gcaagattca acgaataaga acattgcatt
    15001 gcctaccgtt aatttagtaa atatatatga tcaaactcaa gaagatagac cttcttatga
    15061 agaggtagct aaatcggagc ttaaaggcaa cgcctattca catgaaatta aagttaatgt
    15121 tgtgctaaat gctagaaata tcaatgtaga aaatatcgaa acaggaatgt tagcgactat
    15181 tgtttacaaa gagcagactt acaaatctgt tctaactgcc tggcgaatat ccagcagtaa
    15241 aaagaatata gaattgacat ttggtaatgt ccgaagtcgt tttatggatt attttgaaga
    15301 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    15361 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    15421 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    15481 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    15541 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    15601 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    15661 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    15721 actatgctca gttaattcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    15781 cgagctgact ctctctaaag gagaaatttt gtggaggggg actgcgttaa tgcatgatac
    15841 tcaaactgtc caaccttcaa aaaaaatttg gcagacagtt tcaggttttt tgttagtatg
    15901 gctcccatac gaaaacgggc aagcaattga ggatagatat gtaactacgc ctttttataa
    15961 ggagcgtata acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    16021 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    16081 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    16141 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    16201 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    16261 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    16321 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    16381 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    16441 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    16501 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    16561 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    16621 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    16681 gaatcagata agcaagtaaa tgatcgtatt gataaggcaa ataatcgtat tgatcaaacc
    16741 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag cattga
//
LOCUS       DACDBG010000057        16668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDBG010000057 DACDBG010000000
VERSION     DACDBG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.057"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(157..864)
                     /locus_tag="IVZ90_001891"
     CDS             complement(157..864)
                     /locus_tag="IVZ90_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8262929.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="IVZ90_001892"
     CDS             complement(943..1281)
                     /locus_tag="IVZ90_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8262930.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="IVZ90_001893"
     CDS             complement(1392..2354)
                     /locus_tag="IVZ90_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8262931.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="IVZ90_001894"
     CDS             complement(2369..3700)
                     /locus_tag="IVZ90_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8262932.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="IVZ90_001895"
     CDS             complement(3708..4388)
                     /locus_tag="IVZ90_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8262933.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="IVZ90_001896"
     CDS             complement(4607..5086)
                     /locus_tag="IVZ90_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8262934.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="IVZ90_001897"
     CDS             complement(5213..6892)
                     /locus_tag="IVZ90_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8262935.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="IVZ90_001898"
     CDS             7440..8942
                     /locus_tag="IVZ90_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8262936.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="IVZ90_001899"
     CDS             8955..9617
                     /locus_tag="IVZ90_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262937.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="IVZ90_001900"
     CDS             complement(9718..9921)
                     /locus_tag="IVZ90_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8262938.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="IVZ90_001901"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="IVZ90_001901"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8262939.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="IVZ90_001902"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="IVZ90_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8262940.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="IVZ90_001903"
     CDS             complement(13885..14499)
                     /locus_tag="IVZ90_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8262941.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="IVZ90_001904"
     CDS             complement(15105..15482)
                     /locus_tag="IVZ90_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262942.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="IVZ90_001905"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="IVZ90_001905"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8262943.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3593 c         2847 g         5399 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cacttgct
//
LOCUS       DACDBG010000058        16110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDBG010000058 DACDBG010000000
VERSION     DACDBG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.058"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..577)
                     /locus_tag="IVZ90_001906"
     CDS             complement(<1..577)
                     /locus_tag="IVZ90_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8262944.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="IVZ90_001907"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="IVZ90_001907"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8262945.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="IVZ90_001908"
     CDS             1633..3096
                     /locus_tag="IVZ90_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8262946.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="IVZ90_001909"
     CDS             3093..3551
                     /locus_tag="IVZ90_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8262947.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="IVZ90_001910"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="IVZ90_001910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8262948.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="IVZ90_001911"
     CDS             4963..5691
                     /locus_tag="IVZ90_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8262949.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="IVZ90_001912"
     CDS             5709..6905
                     /locus_tag="IVZ90_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8262950.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="IVZ90_001913"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="IVZ90_001913"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8262951.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="IVZ90_001914"
     CDS             7890..8831
                     /locus_tag="IVZ90_001914"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP8262952.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="IVZ90_001915"
     CDS             9007..9486
                     /locus_tag="IVZ90_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8262953.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            9499..10389
                     /locus_tag="IVZ90_001916"
     CDS             9499..10389
                     /locus_tag="IVZ90_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8262954.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="IVZ90_001917"
     CDS             10376..11185
                     /locus_tag="IVZ90_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8262955.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="IVZ90_001918"
     CDS             11199..11600
                     /locus_tag="IVZ90_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8262956.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="IVZ90_001919"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="IVZ90_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8262957.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(13156..13431)
                     /locus_tag="IVZ90_001920"
     CDS             complement(13156..13431)
                     /locus_tag="IVZ90_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8262958.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            13539..14303
                     /locus_tag="IVZ90_001921"
     CDS             13539..14303
                     /locus_tag="IVZ90_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8262959.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            14426..14929
                     /locus_tag="IVZ90_001922"
     CDS             14426..14929
                     /locus_tag="IVZ90_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8262960.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            15338..15979
                     /locus_tag="IVZ90_001923"
     CDS             15338..15979
                     /locus_tag="IVZ90_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8262961.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
BASE COUNT         5193 a         2654 c         3490 g         4773 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg atgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg acaagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga
    12721 aaaaaagaca aaaaatcaag aaagaaatac ttggaaagaa aagagtgatt ccaagtgttt
    12781 ctttcttatt taaaaaacta aaaacttttt tgttctgtct ggaaaatatg ggttaagcaa
    12841 atttattgca agttatagat cagctttttt actacctatc tttaatactt gaggcaaagc
    12901 gtctcaaaaa caaccttatc gacggtgttc aaaagtcagt ttctcggtat taagtcaaat
    12961 tttatgcaaa aatgtggaaa gccattttca caaaattctc cttactactc aaaactaagc
    13021 gactttctca caaccttatt gacggtgttc aaaagcttgc atctacggca ttaagtccag
    13081 cctgttgaaa aatatggaga gctatttttc acaacctgtc cttaatgctt gatgcagacc
    13141 agctttttca caaccttatc ccaccacatc aaatttcttt cgttcctcaa tcatcgggtc
    13201 ttcttggaca tccacatact gcactcgtcc acttgggctc ggagatttct tgacttcttc
    13261 aataaatttc tggatgatgt cgtcattgcc gattgcttcg atcgtgactg agccgtcatc
    13321 ttcgtttttt accgttccag tcacgcctag ttgatcagcg accatcttgg tcatgtagcg
    13381 aaatcctacg ccttggacgc gtccttggac gttcatttta actttgcgca tgttcgtcac
    13441 ctttctttcc tattttataa attaatgata ccgatttttt taggatgaaa caagctcaag
    13501 cacctattcc atgatataat catggggagg tgaggaatgt gaaagagatc caatcagcaa
    13561 aaaatccaag aatcaaagaa ttaaaaaaac tacataaaaa gaaatatcgg gaagaaaagg
    13621 aagaatatct tttggaaggg ttccatctga ttgaagaagc tgcgtcgtct gatgcagaaa
    13681 taaaagaact ttttgtgaca cagcgaggca tgaaagaatg gggagactgg ctggccgcaa
    13741 acaaagagat ttctactttt ctagtttcgg atgaggtcat ggatgctgta tcggatctac
    13801 ctacaccaca aggaatctta gcagtgatgg cgttaacggc tgaaggcacg ccagattttt
    13861 caggaggatg gctgttatta gataatgtac aagatcctgg aaatgtaggg acgatgatcc
    13921 gtacggcaga tgcatttgat atggctggtg ttttattagg caaaggaacg gcagatattt
    13981 atagtacaaa ggtgctgcgc agcatgcagg gaagcaatta tcatttgcct gtgttgcaaa
    14041 aagatttgac agaagtgatt cctttaatga aagacaaagg gtttacagtg tatggcacag
    14101 aattaaatga agaagcacac gccttagaca aagtagaaaa atcagataac ttcgcaataa
    14161 tcatggggaa cgaaggtcag ggggtaagtc aggaaatctt gtctttgact gatcagaacg
    14221 tatatatccc gatgaaagga aatgcggaat cgttgaatgt tggggttgcg gcagggattg
    14281 ttctttatca tttttcttta tgattaagga actattaagc tttggctatt ttatccaagg
    14341 cgactagaat aatccgaaca ctggtggtat acttagatca atttcagaag aaaatgaaat
    14401 tcaaaaaggt ggcagatcga taattatgac agaaaaatgg cgtgaagacg aagaatatat
    14461 gtcttatgta gaagatcttt tagaaacaga agcagtgaaa aaattagcga attacacaca
    14521 gcatgttcat tccactcgtt tggagcattc catcagtgtt tcttactata gttatctgct
    14581 cgcaaaaaaa tggggtggca atgctaaagc aacagcgcgt gctggacttt tgcatgatct
    14641 gttctactat gattggcgta cgacgaaatt tgatgaaggc acacatgcgt atatccatcc
    14701 aagaatcgct gtaaagaatg cagaaaagat caccgatttg tcggatcttg agcgagatat
    14761 cattttgaag cacatgtggg gcgcaacgat tgcaccgcct aagtacaaag aaggatatat
    14821 cgtaacattc gtagacaaat actgcgcagt caaagaagcc gctcagccta tgtcagcaag
    14881 tatgcgtaag agatggcaac gttacttcgg aaaagaatcc tcgatttaac aggtaaaacc
    14941 aataatagac catattgagg ttgtgacaga agtgggtcag cttcaagaaa taagaaggaa
    15001 tttacgaaaa ttgcttctca aatttttgtg aaattccggc ttattttttg aagaagctac
    15061 ttctggaaca ccgtttgttt gtagaaaggg actttgacac gacttttgtc acagtccttt
    15121 ttctgtttgc agttgactat ctgctcaaga tacggtttac ttttatttat cgaatgaatc
    15181 atgtacatag aagaatgaac attaaagggg gtgatgataa gatgcaaaac gtatcttatt
    15241 taaaagagtc cgggatcttc aggtaacaca tactttgcag aagatcactt ttttctacaa
    15301 agtatgcact ttggttagaa gaaaggactt taaaatcatg gaacaaacaa tgaaaccata
    15361 tcaatatttt tttataaaaa gagaagtaga gcagttatta aatgcgtact catctgtcaa
    15421 tgactcaaaa acggtacaaa cggttcaggc tctagcagct gaaaagattc gcgatgtatt
    15481 aggttacgaa ccatcagaaa tcgatacgtt tttaacgaca gttttagatg taaaactgac
    15541 aaaaacgcaa gcagaacgag cattggagga gctgaagcaa atcgtccagc cgtttatgca
    15601 gccgagcaaa ccacaaatcg agaaattatt ccgaaaagtg aaaaaactca aacaacccgc
    15661 ttggaatgaa atcgacttgc aagcacatac gtacatcggt tggaatgatc cagggacgca
    15721 aaaaaaattc atggtttatt acgaagatgg aaaattaaaa gggatcagcg gtactttatc
    15781 accaacgatt aataagggaa tctgtgcgat ctgtcagaaa acatcgaatg tctcgctctt
    15841 tctttctaca acgaaagccg gttctgatgg cacgtatacg aaaaagggga attatatttg
    15901 tcatgatagt gatcaatgta atcaacagtt aacgaaattg gctcctttct atgaatttgc
    15961 tgaacgtgta aagaaataac gataatttta ttgtttttta cagaaaaata tgctatcttt
    16021 taatggaagt gaataggtga tggtgttcgc ctttaaatgc taacgtaaga tagctaatga
    16081 cgcctactaa agtctggggc cagccccttt
//
LOCUS       DACDBG010000059        16010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDBG010000059 DACDBG010000000
VERSION     DACDBG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.059"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            611..997
                     /locus_tag="IVZ90_001924"
     CDS             611..997
                     /locus_tag="IVZ90_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8262962.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1091..1282
                     /locus_tag="IVZ90_001925"
     CDS             1091..1282
                     /locus_tag="IVZ90_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8262963.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1796..2128
                     /locus_tag="IVZ90_001926"
     CDS             1796..2128
                     /locus_tag="IVZ90_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8262964.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2424..2579
                     /locus_tag="IVZ90_001927"
     CDS             2424..2579
                     /locus_tag="IVZ90_001927"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8262965.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2855..4195
                     /locus_tag="IVZ90_001928"
                     /pseudo
     CDS             2855..4195
                     /locus_tag="IVZ90_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4436..6052)
                     /locus_tag="IVZ90_001929"
     CDS             complement(4436..6052)
                     /locus_tag="IVZ90_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8262966.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6170..6388
                     /locus_tag="IVZ90_001930"
     CDS             6170..6388
                     /locus_tag="IVZ90_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8262967.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6583..7044
                     /locus_tag="IVZ90_001931"
     CDS             6583..7044
                     /locus_tag="IVZ90_001931"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8262968.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7237..7476)
                     /locus_tag="IVZ90_001932"
     CDS             complement(7237..7476)
                     /locus_tag="IVZ90_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262969.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7500..9023)
                     /locus_tag="IVZ90_001933"
     CDS             complement(7500..9023)
                     /locus_tag="IVZ90_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8262970.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9041..9163)
                     /locus_tag="IVZ90_001934"
     CDS             complement(9041..9163)
                     /locus_tag="IVZ90_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8262971.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9193..10176)
                     /locus_tag="IVZ90_001935"
     CDS             complement(9193..10176)
                     /locus_tag="IVZ90_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8262972.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10200..10349)
                     /gene="rpmG"
                     /locus_tag="IVZ90_001936"
     CDS             complement(10200..10349)
                     /gene="rpmG"
                     /locus_tag="IVZ90_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8262973.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10370..10747)
                     /locus_tag="IVZ90_001937"
     CDS             complement(10370..10747)
                     /locus_tag="IVZ90_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262974.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10779..10958)
                     /gene="rpmF"
                     /locus_tag="IVZ90_001938"
     CDS             complement(10779..10958)
                     /gene="rpmF"
                     /locus_tag="IVZ90_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8262975.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10973..11242)
                     /gene="rpsN"
                     /locus_tag="IVZ90_001939"
     CDS             complement(10973..11242)
                     /gene="rpsN"
                     /locus_tag="IVZ90_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8262976.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11765..13537)
                     /locus_tag="IVZ90_001940"
     CDS             complement(11765..13537)
                     /locus_tag="IVZ90_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262977.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13491..15245)
                     /locus_tag="IVZ90_001941"
     CDS             complement(13491..15245)
                     /locus_tag="IVZ90_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8262978.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15355..15924)
                     /locus_tag="IVZ90_001942"
     CDS             complement(15355..15924)
                     /locus_tag="IVZ90_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8262979.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         5187 a         3051 c         2424 g         5348 t
ORIGIN      
        1 aacttacttc attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt
       61 acattttatc accgagaact catcattttt catcatgatg aatactattt acatatatct
      121 agttcaaaga caataattat tatgatccgt tattatctaa gcactataca gataatagga
      181 atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt
      241 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
      301 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
      361 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
      421 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
      481 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
      541 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
      601 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
      661 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
      721 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
      781 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
      841 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
      901 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
      961 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
     1021 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
     1081 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
     1141 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
     1201 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
     1261 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
     1321 tttcaaattt cgaacaataa atttgaaact atcttcttta taattataaa gtatcgattt
     1381 tacagcgttc tataatgatt catagaatct aaaacgaatg attttaatta tcaacgtttt
     1441 ttaattcgtt tttagcttaa aaataacttt tgtcacagtc tcactcctaa aattacttta
     1501 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
     1561 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
     1621 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
     1681 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
     1741 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
     1801 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
     1861 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
     1921 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
     1981 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
     2041 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
     2101 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
     2161 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
     2221 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
     2281 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
     2341 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
     2401 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
     2461 atcggtcttt tatttatcaa gagctgttga acgaccacca catcacacat tcagtgagcc
     2521 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     2581 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     2641 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     2701 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     2761 ccaatacttt ttcttttttc taataaacgc aaacagacac cttaaaatcc tttagaataa
     2821 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
     2881 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
     2941 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cggaggaatg
     3001 ctttcatcgc cactatcaaa atgaacaaac gatcattaaa tagggctgga aaaaagtatc
     3061 catcttattg aatgatgtca gtaactacaa gaccattctg cggattaaca aacagcgctt
     3121 caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagtg atcgtcgctg
     3181 ctccattgct cgcagagtca aacaagccat tcttgagcta ttaagcgaac ctctttctat
     3241 gtctttgatc gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag
     3301 ccttcggcct aaaaccattt ctttgagcca gctgttacca gaagtggtgt gtttcgatga
     3361 attcaaatca gtgaaaaatg tctcgggagc catgagcttt gtcatgatgg acggcaagac
     3421 ccatcaactg attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt
     3481 gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg gtttcagatt tctatacacc
     3541 ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca
     3601 tattagccaa cacattggtc gggcttttac caaccaccga attcaagtaa tgaagacctt
     3661 taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac tggaaactac ttcaaaagaa
     3721 tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac
     3781 agaagcagag atagtcgatc ggctattatc ttatgatgat tccttaaagc gaggctacga
     3841 agtctaccag gattttcttt ccgttatcag aagacaagat gttcctgaat tcgtcgcgct
     3901 cctaaaagaa gactacaagg agttgccaga gcactaccaa ccagtgttca cgaccttcaa
     3961 gaaataccaa acagaaatca aacgagcgtt gcgcgttccc tattccaacg gccctataga
     4021 atgtttgaat aaccatatca aagtactaaa acgaattgcc tatggctata gaaactttca
     4081 aaactaccga gaacgaatct ttttatatcg aggaaaatat tttaagaaaa ctaagaatac
     4141 tacccaactg actaaagcca gaacaacaac acggctagac aagatagctg tctagccgtg
     4201 tgagtatctc gattattcta ttgaatttct tcaccaacac tcattgacga agagccaaaa
     4261 taagtaatct aaccgactgt taactaaacc attgcaggtc ccgttaaaga tacttgtgcc
     4321 tgttgtcacc gcttcttttt cttgtccaca agcgcttaac acacttaata aagcccctgc
     4381 tattattaat aataatatct ttttcttatt aaaaacctcc tcatctattt ccagattagt
     4441 aaatgtagta atctgtgctt tcttttttct caatactaat aactaactta tggggtaaac
     4501 aaatactcgt ttgaccgggt tttgagatcc atccagttct aaccgcgatt tgatcaggac
     4561 tattatcttc tttaactcga attcttccat ctttaatctc aacaatatta tattgtccct
     4621 tcgcaggatg ataattcacg agtttatgat tgatcttatc caaatcgaag cgatccattt
     4681 ccttgccatc aattcgaacg atggcatatt tgatagcatc ttcagttgtt tcattctcct
     4741 tttctataga gaatacaaat aagggaataa aagaggtaat aatcaaaatg ccaataatta
     4801 tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt cattaacgat atctggctaa
     4861 acatattaga ctttttccat ggaaatggtg aaatttttcg gaaattatct gcattaagag
     4921 aatcagttaa acaaccctca agttgttcct taatacattg ttgatccatc ttcataccaa
     4981 taaaaactag tttattaatg cgttcaccaa acaacggatg ccaggcttcc ttcaattcgg
     5041 gatttttttg tataatcatt tgacactcaa ttttaggtaa actagctacc caataattta
     5101 atgattcaat acttttatag ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt
     5161 gggcaaacca aagaaaccct tttgctcttg taatattgcc aggaaaattt ttatccgtaa
     5221 gatgggcaaa tttttgtgcg tcaaggagta accgatttcg atagataaaa cttgagatac
     5281 catattcttc tgtttcagga atatgatttt gatagccaag ctctaactct ttgaaccagc
     5341 ccactgaata ggaagtctca tctatattaa aaagatttgt atttagaatt ttcgataagt
     5401 ctacttcacc tttaattgtc ctaattattt tggcattgga ctgtaactta gcaattagac
     5461 cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact attatttaaa agtaatatgt
     5521 tacaacactc gaattgatca attaacaagc cacgaatatc ctgttcttct tcaactacat
     5581 taccttcagc ttttctttcc tgaatctttt cacccgtttc gaaattatcc caaaaacgtt
     5641 ttacatccac tactataata atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta
     5701 aagggttttt agctaatata attgcttgaa taattggaat tggtttgctg attcctgaag
     5761 cttcaataac aatttgattc aagtcagaaa attgcactaa ttgatctagt tcaacaatca
     5821 aatcttcgcg taatgtacaa caaatgcatc cattcggcat cgaaacaagt ttttctccag
     5881 ttcttttaaa acctttcttg tcgactaata ccccatcaac atttacttcc cctatgtcat
     5941 ttacaatgac agcaatttcc tttccgtgat caccgtacag aatatgattt agaagtatcg
     6001 tttttcctga accaagatag ccagttaaga ttgtaatagg tatttttttc atagggaacc
     6061 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     6121 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     6181 tcgtcatctt actatactcg aacgagaaag aatctttctg cttcacgaag aaggattttc
     6241 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     6301 aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     6361 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     6421 ttatttgtcg attttcagtg gtcgccagag gaaatctcga atcgtttgaa gcatgaacat
     6481 atggactttt caattagttt tatcacgatc taacgtgcaa tttgcaatga tcttttcgat
     6541 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     6601 acaagacata caaaaaatca tgtcgagtct cgtggaaaga tttgccttac tcattcgatc
     6661 catggccgtc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     6721 gtagctggca aaactggtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     6781 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     6841 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     6901 gaattctcca atcatttcgc agttacagaa gcattgaata atatgccctt ttattttcca
     6961 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     7021 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     7081 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     7141 ttgataaagt gttgcgcttg atttgacaat tcaagacata ataaaaagca gatcaaatcc
     7201 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     7261 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     7321 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     7381 tgtactccca tttaatctca tttgctttgc aaatcgataa cgccgataag catcaatatt
     7441 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa aattgaattt
     7501 taatgagcca tcatttcttg agcaatttca tgtttagaca atgaagctgg atagaaagta
     7561 ggccaattat gcaattcatt atagagtttt tcttgacttt ctccaccaaa gaaaatatgg
     7621 aaatgctctg ccttacttgg tgcaattcca tgatcactaa attgaatata tttaaaacga
     7681 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     7741 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     7801 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     7861 taataatctt tgtactcggc agctgtcatg tctttattga tttttgcttt gtaatcgaaa
     7921 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     7981 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     8041 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttttct
     8101 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     8161 tttgtaagac tttcaagagg attcagtact tccaatttta tcccagcttc gttggccaaa
     8221 gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     8281 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     8341 ccagcaatag gaacttgttt caatccataa tccaaagcta aatatccaaa ggctgcgtgt
     8401 tgcgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     8461 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     8521 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     8581 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     8641 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     8701 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     8761 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     8821 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     8881 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     8941 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     9001 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     9061 tatttcgttt atgttctttg ataattttta atgccctttt tctagtttta atattcgggc
     9121 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccccaat
     9181 cactacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     9241 ttcatccaac atttttccga ttagaataat agtggtttgg tctttttgtc gagtggtgta
     9301 ttgaaaatta acctgttcgt ttacctcttg catggcaact ataaaatcct gctcttgtaa
     9361 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccagtcaat
     9421 ccattggagt aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     9481 ctcatgtact tgatggtgtt gatgatagta tttgccatta tagtgatttt ctgatgaatc
     9541 actttcaaaa actaagggat gattaaattt tttcaaatta taaaaatcct ttttttgaat
     9601 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     9661 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     9721 tgccatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     9781 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     9841 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     9901 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     9961 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
    10021 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
    10081 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
    10141 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
    10201 tatttcactt ctttaaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
    10261 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
    10321 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
    10381 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
    10441 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcgata cattgaaata
    10501 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
    10561 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
    10621 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
    10681 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
    10741 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
    10801 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
    10861 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
    10921 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
    10981 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
    11041 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
    11101 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
    11161 ttaattcctg acgtttatct gcatactttt caatcatcgc catttgtttc tttgctttag
    11221 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacacctc
    11281 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
    11341 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
    11401 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaggctctg aaaaataaga
    11461 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
    11521 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
    11581 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
    11641 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
    11701 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
    11761 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
    11821 ttatgagatt tttgtatatt tctgaccttc gatacaactc attatgagta ccttgatcaa
    11881 ctagttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
    11941 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
    12001 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
    12061 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
    12121 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
    12181 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
    12241 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
    12301 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
    12361 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
    12421 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
    12481 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
    12541 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
    12601 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
    12661 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
    12721 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
    12781 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
    12841 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
    12901 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
    12961 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
    13021 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
    13081 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
    13141 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
    13201 gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct ttataagtac
    13261 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacaa
    13321 acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat tcgtctatca
    13381 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa ctagaaatag
    13441 aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt ttataaaaac
    13501 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
    13561 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
    13621 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
    13681 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
    13741 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
    13801 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
    13861 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
    13921 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
    13981 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
    14041 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
    14101 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
    14161 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
    14221 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    14281 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    14341 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    14401 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    14461 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    14521 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    14581 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattccg
    14641 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    14701 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    14761 agaaccaatc caattttaaa gttaatgaaa aagcctaagg caactgataa aacaggtgtt
    14821 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    14881 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    14941 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    15001 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    15061 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agactgaaga
    15121 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    15181 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    15241 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    15301 ttccgtaata gcgtaaatta gagatgtaca aacagataaa aggtaaacta cgttctattt
    15361 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    15421 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    15481 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    15541 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    15601 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    15661 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    15721 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    15781 gattccagat aataatccaa aaatcccggg ggatatgctg tgaccaaacg ccccaccaat
    15841 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    15901 aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta
    15961 aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt
//
LOCUS       DACDBG010000060        15949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDBG010000060 DACDBG010000000
VERSION     DACDBG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.060"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            310..1449
                     /locus_tag="IVZ90_001943"
     CDS             310..1449
                     /locus_tag="IVZ90_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8262980.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1516..2091)
                     /locus_tag="IVZ90_001944"
     CDS             complement(1516..2091)
                     /locus_tag="IVZ90_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8262981.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2096..3070)
                     /locus_tag="IVZ90_001945"
     CDS             complement(2096..3070)
                     /locus_tag="IVZ90_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8262982.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3094..3138)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3353..4321
                     /locus_tag="IVZ90_001946"
     CDS             3353..4321
                     /locus_tag="IVZ90_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8262983.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4434..4712
                     /locus_tag="IVZ90_001947"
     CDS             4434..4712
                     /locus_tag="IVZ90_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8262984.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4830..5066
                     /gene="secG"
                     /locus_tag="IVZ90_001948"
     CDS             4830..5066
                     /gene="secG"
                     /locus_tag="IVZ90_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8262985.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5178..5924
                     /locus_tag="IVZ90_001949"
     CDS             5178..5924
                     /locus_tag="IVZ90_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8262986.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5927..8284
                     /gene="rnr"
                     /locus_tag="IVZ90_001950"
     CDS             5927..8284
                     /gene="rnr"
                     /locus_tag="IVZ90_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8262987.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8306..8770
                     /gene="smpB"
                     /locus_tag="IVZ90_001951"
     CDS             8306..8770
                     /gene="smpB"
                     /locus_tag="IVZ90_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8262988.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9072..9791
                     /gene="atpB"
                     /locus_tag="IVZ90_001952"
     CDS             9072..9791
                     /gene="atpB"
                     /locus_tag="IVZ90_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8262989.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9844..10059
                     /gene="atpE"
                     /locus_tag="IVZ90_001953"
     CDS             9844..10059
                     /gene="atpE"
                     /locus_tag="IVZ90_001953"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8262990.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10150..10674
                     /gene="atpF"
                     /locus_tag="IVZ90_001954"
     CDS             10150..10674
                     /gene="atpF"
                     /locus_tag="IVZ90_001954"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8262991.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10661..11203
                     /locus_tag="IVZ90_001955"
     CDS             10661..11203
                     /locus_tag="IVZ90_001955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8262992.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11226..12782
                     /locus_tag="IVZ90_001956"
     CDS             11226..12782
                     /locus_tag="IVZ90_001956"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8262993.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12796..13698
                     /locus_tag="IVZ90_001957"
     CDS             12796..13698
                     /locus_tag="IVZ90_001957"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8262994.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13721..15127
                     /gene="atpD"
                     /locus_tag="IVZ90_001958"
     CDS             13721..15127
                     /gene="atpD"
                     /locus_tag="IVZ90_001958"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8262995.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15141..15563
                     /locus_tag="IVZ90_001959"
     CDS             15141..15563
                     /locus_tag="IVZ90_001959"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8262996.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5294 a         2856 c         3443 g         4356 t
ORIGIN      
        1 atatcagcag gtagagagtt tttttgcaac tagcttctgt atatgatgtt tacaaagaat
       61 ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga
      121 gcttttaagg atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa
      181 ataggaggga agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt
      241 ggttgctcct atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga
      301 gaggattaga tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa
      361 tcagtgaatg gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat
      421 gacataacag gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac
      481 caaaagggcc aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct
      541 ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg
      601 tacggacgtg gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc
      661 gaactgaaag agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga
      721 gaagaagtgg gtgaactagg tggggaacag ctcacgaaag caggctatgt agatgactta
      781 gatgccttga tcatcggaga gccaactaat tacagtttga tgtatacgca catgggatca
      841 atcaactata cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt
      901 tataatgcaa tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac
      961 ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc
     1021 agtggcggga atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga
     1081 tcgattccag aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa
     1141 ttgaatcaag agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc
     1201 aaagctgatc cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta
     1261 cctttagttg gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc
     1321 ttcgactttg tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat
     1381 gttgaaatcg ataattattt ggatatgatt gaaaaatatc aaggaattat tttatcctac
     1441 ttagcgtaag atttttttaa taaatgaaaa aacagagcca gattagcatt attttgctaa
     1501 tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt gtggcaacat
     1561 agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt ctgtacctgc
     1621 ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata gctgaacgat
     1681 catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga tcgaagaaac
     1741 gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc tgtttttatc
     1801 tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa tttggattgg
     1861 cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc tggacaatcc
     1921 tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc
     1981 gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat tgatcccaat
     2041 acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca ttttgttagc
     2101 ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg
     2161 ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta ctgttagcag
     2221 gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt ccttgagttt
     2281 ccacagcagt gaagcttttg aaaccttcac agatggcccc ctcgataaaa taagcaacag
     2341 ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg aagtcaaaat
     2401 aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga cagcagtatt
     2461 cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc atttctatcg
     2521 tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag taattgtatt
     2581 cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag acaaaacgat
     2641 ccatatgttt cccaatattt gggttgactt tcaaagcaaa agcgatattg gtcaaaggac
     2701 ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa aaatcaacgg
     2761 catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc cctaaaccat
     2821 ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt tccattcctt
     2881 gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca ttctgcgtac
     2941 ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg atatcagggg
     3001 atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta
     3061 cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta ttctgggaag
     3121 tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag tatactggct
     3181 tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac tttaagtaaa
     3241 tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc ctatggatcg
     3301 gtacattgga taatagttag attatagttt ggcagaaagc ggcgggtgta caatggttat
     3361 tttttatttc attcttattc tgataattat agggatcagt taccattttc tttcttattt
     3421 ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt atgaagacac
     3481 tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac gacaaaaatt
     3541 agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca aaagttatga
     3601 cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata aatgggtcat
     3661 ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag gtaaaagata
     3721 tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag aaagtgaagg
     3781 agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga tccaactcat
     3841 cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg gagcttctac
     3901 gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta ttttagacag
     3961 cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca ctgtctttcc
     4021 taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg gttactcctt
     4081 aaagcaagca tcagctacga gacagcttgg cagcaaccat ctgcctttat tggtcattca
     4141 tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa atgctacggc
     4201 aggggataaa gcactgctgc ttgttccaga agcagaacac ttggaagcat cgatgaaaga
     4261 tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa cgatgaatta
     4321 attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag atcaggagac
     4381 tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg catatggaaa
     4441 tcaaagaaga aaaaaatcgt cttgctttat tcaatgatga acaacaagag atcggagaaa
     4501 tgacatggtc agatgcgggc ccagacatca tgatcattga ccatacattt gtagatccag
     4561 catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa ttagcaagac
     4621 gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt gaaaagaaac
     4681 cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt ttgaactttc
     4741 agctgttatt ccatttatat agagttgtca cggactttta tctatgataa agttaaagag
     4801 acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga atcgtgatcg
     4861 ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa aatagtgcag
     4921 cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct cgcggctttg
     4981 aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc ttattatttg
     5041 tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg cgggcggctt
     5101 tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg taaaatgagg
     5161 cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa catggaaagc
     5221 gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga atgctcgcac
     5281 gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga cacggaacaa
     5341 tggagccata tgatattttg caagaaaaag cagaaagctg gtgggcagac actaaaaaag
     5401 ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga ttatcaatgg
     5461 gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc ggttttttct
     5521 gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa tacgtacgtc
     5581 aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca gttgcttacc
     5641 gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc gtagaaagta
     5701 ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat gaaatgatcg
     5761 accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg acacttcgat
     5821 ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa ttagaaaaag
     5881 acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga caaaagaaac
     5941 gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt tttctatgga
     6001 agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac tagtacaaac
     6061 gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa aagtgaaact
     6121 tcctttaaaa ccaatcttga tcgaaggaac atttcgagca aatgaacgag gatttggatt
     6181 tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga ctggttttgc
     6241 aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag atatggatcg
     6301 cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt tagcagggga
     6361 attcgttgct tattcagaag aagaaataca agaaacggat ctttacggcg ttgttatccc
     6421 gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag ttcgtccagt
     6481 tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt atgcgactag
     6541 tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca tggatatcct
     6601 ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat tgtctgaagc
     6661 agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag atttaagaaa
     6721 tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg cagttacggt
     6781 taaaaaatta gataacggta attattttct tggagtacac attgcagatg tttcttacta
     6841 tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa gtgtatattt
     6901 gactgatcga gtgattccga tgatcccgca acgtttatcg aatggtatct gttcactgaa
     6961 tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa atggacaagt
     7021 agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga cctacacagc
     7081 tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg ctcagctcgt
     7141 acctatgttc caagaaatgg gtgaattgca tcgaatcctt gaaacgatgc gaatcaaacg
     7201 tggcgcgatt tcttttgagg atcgcgaagc aaaaatcctt gttgatgctg aaggacaccc
     7261 aaatgatatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat ctttcatgct
     7321 tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt ttatttaccg
     7381 aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg cttcagcttt
     7441 gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac agcatgtgat
     7501 cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc taagaagcat
     7561 gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg cagattacta
     7621 cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc gtctgatccg
     7681 ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctacgg aacttcctga
     7741 aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac gtgaagtaga
     7801 ttccatgaag aaagcagaat tcatgatgga caaagtgggg gaagaattcg atgggatcat
     7861 cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag agggattgat
     7921 ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt tagctcttgt
     7981 aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa aagtagtcaa
     8041 agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag aggttacagc
     8101 gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa aacaagcagg
     8161 aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta agcaaacgtt
     8221 cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga aaaagaaaaa
     8281 ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa ttgcacaaaa
     8341 caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga tggtcttgca
     8401 aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg gatttattcg
     8461 cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg aacaaggaaa
     8521 tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa aacaaatcat
     8581 ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa aagtctatat
     8641 tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat cttatgataa
     8701 acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat taaaaaattt
     8761 ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt acttaatgat
     8821 aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt cttagatcag
     8881 gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa agtgtctaat
     8941 tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg tggttagaaa
     9001 ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc ggggaaaaga
     9061 ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt tggtttgatg
     9121 gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta gcttactttt
     9181 ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa tgggtcattg
     9241 attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc aatttccatt
     9301 tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt ttagtaacaa
     9361 aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac ccgtttgtta
     9421 ctttaacttt ggcatttata atgatcttac ttactcactt atttggggtg aaaaagttag
     9481 gcttcaaagg atatttcgta aattcgttct tgaaaccata tagttttatg tttccgatga
     9541 aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat gggaatattt
     9601 atgcggggga agttttgcta accttgattg caaaaatggt aataagctta ggctggttct
     9661 cattgccgtt agcaattcca ttagagatgg tctggatcgc attctccttg tttatcggaa
     9721 gcatccaagc atttgtcttc gttactttat ctatggttta tatgagccat aagattgaag
     9781 tggaagaata atattcgttt taaaaacaaa ctactattta tctaatttag gaggaaaaca
     9841 attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg agccggttat
     9901 ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc tgaaatgtct
     9961 ggtcaattcc gtacaacaat gtttatcggg gtagctctag tcgaagctgt tcctatctta
    10021 ggggtagttg tcgccttgat tctagttttt ggtgtttaat tgaatcagta ttaaggcaga
    10081 aacaatttgg tttgttactg cttttttaca tgaaaactag aagtacttca aagaaaggaa
    10141 ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat gttaggaaat
    10201 gtcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca ttttgcttgg
    10261 ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga tttggattct
    10321 gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca acaattgtta
    10381 gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg agaattaagc
    10441 cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag taaagcccaa
    10501 gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga tgttgcagaa
    10561 ctatctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga aatgcacgaa
    10621 tcattaatca atcaatatat cgaaggtcta ggttcttcaa atgaaactag ataaatatac
    10681 agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa ataaagcaga
    10741 agaaatttat caagatctcg taagcttacg tcaaatctat gaacaagtac ctggattagg
    10801 taatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca tggacaagct
    10861 tgttagtggc tatgatggaa tcgttaaaaa ctttttagaa gttgtttatg cgtataaccg
    10921 aatggatgat cttttgttca tgatcgaaga atatgaacgt cgatatgatg aacatcaagg
    10981 tttgctgttg ggaagtgtca caacagcagt tccactttca gacgaacaac gaatacaatt
    11041 agaaaagaat gttgcaaaaa caatgaacta tcaatcagtc gaattaaaac aaatcgttga
    11101 ttcttctatt attggtggcg cgattgttga agctaaccat cgagtaatcg acggaagcat
    11161 tcgcacgcaa ttagaaaata tgcgtaatca attaaataga tagtacaaag aggtaggtga
    11221 atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa attgaaaatt
    11281 atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt gacgggatcg
    11341 ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt tcaaatggct
    11401 catacggaat ggcgcaaaac ttagaatcaa atgatgtagg tatcattatc cttggcgatt
    11461 tcgagtccat tcgtgaagga gataaagtaa aacgaacagg aaagatcatg gaagttccag
    11521 ttggagaggc cttgattgga cgggtagtta atccgctagg tcagccaatt gacggactag
    11581 gtgaaatcgt tactgacaaa gctcgtccag tagaagcaat ggcaccaggc gttatgcaac
    11641 gtaaatctgt taacgaacca atgcaaacag gtctaaaagc aatcgacgct cttgtaccaa
    11701 tcggacgcgg ccaacgtgaa ttagttatcg gtgaccgtaa aacagggaaa acttccattg
    11761 cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat gtagcaatcg
    11821 gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat ggcgcaatgg
    11881 attacacaat cgttgtgaat gccggtgcgt ctcaaccagc gccgttgctt tatatcgcac
    11941 catatgctgg tactgcaatg ggtgaagagt tcatgtacaa cgggaaacat gtcttgatca
    12001 tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg ctgcttcgcc
    12061 gtccaccagg tcgtgaagct tatccagggg atgttttcta cttacactca cgcttattgg
    12121 aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc ttaccatttg
    12181 ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt tcgatcactg
    12241 atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca gcggttgatg
    12301 ccggactatc tgtatctcgt gtagggggtt ctgcgcaaat caaagcaatg aaaaaagttg
    12361 ctgggacttt acgtctagac ttagcaagtt accgtgaatt agaagcattt acacaattcg
    12421 gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt acagtagaaa
    12481 tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg attttgtatg
    12541 ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac ttcgaacatg
    12601 aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc cgtacaacaa
    12661 aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa gacatgttct
    12721 tagctacaaa aggaaataat tcctctacag aagataaatt gaaatccatt caaaatgcat
    12781 agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat cgcttcaacg
    12841 aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa gttgacaaaa
    12901 tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga aatcgttacg
    12961 catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg ggatatcaat
    13021 tacaacagca tgttgctcag ccgtcctgtt aagaaaacgg gttatatcgt catcactgca
    13081 gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat gtctatttta
    13141 gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg aactggtgca
    13201 gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct atctgatcag
    13261 ccaagctttg atgaagtccg caaaatcgta aatatggcaa caactatgta ccaaaacgaa
    13321 gtattcgatg aattatacgt atgttataac catcatatca attcgctaac tagtcagttt
    13381 cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac tactttcgaa
    13441 caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt gcctcagtat
    13501 gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca tgctgctggt
    13561 atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga gctgacggtt
    13621 tcttacaacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat tgttgccgga
    13681 gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag gcaagattgt
    13741 tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct taccagatat
    13801 caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg ttcttgaagc
    13861 agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa cagatggttt
    13921 acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg taggtaaaga
    13981 aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa cacctttccc
    14041 agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg aattaagtac
    14101 aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc catatttaaa
    14161 aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac tgatccaaga
    14221 actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg gtgtaggtga
    14281 acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag ttatcgaaaa
    14341 aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc gtgttgcttt
    14401 gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg tactgttgtt
    14461 tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct tgttaggacg
    14521 gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc aattgcaaga
    14581 acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct atgtgccagc
    14641 cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg cgacaaccaa
    14701 cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat tggcttcttc
    14761 ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag caacagaagt
    14821 ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct taggtatgga
    14881 tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc aattcttctt
    14941 gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat atgtccctgt
    15001 agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg atcttcctga
    15061 agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga aaacactagg
    15121 ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta actcctgccg
    15181 gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct ggtgagcttg
    15241 ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa gttcgtgtaa
    15301 aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga atcatggaag
    15361 ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt gatatcgatg
    15421 tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa gcaaaagcaa
    15481 aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca atcaatcgta
    15541 tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt taagaacagt
    15601 aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt tacgaaaatt
    15661 gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt ctggaacacc
    15721 gtatatcgga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac aaatggaaaa
    15781 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga ttgaacaccg
    15841 tttatttgga ataagggact gcgacaagac ttttgggctc tttgtcaata aggactgatg
    15901 agttgtgcaa aatcaaatct gagtcagaat gaaccacatt ctggctcag
//
LOCUS       DACDBG010000061        15872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDBG010000061 DACDBG010000000
VERSION     DACDBG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.061"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(343..2313)
                     /locus_tag="IVZ90_001960"
     CDS             complement(343..2313)
                     /locus_tag="IVZ90_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262997.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2328..3194)
                     /locus_tag="IVZ90_001961"
     CDS             complement(2328..3194)
                     /locus_tag="IVZ90_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8262998.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3184..3417)
                     /locus_tag="IVZ90_001962"
     CDS             complement(3184..3417)
                     /locus_tag="IVZ90_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8262999.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3429..5585)
                     /locus_tag="IVZ90_001963"
     CDS             complement(3429..5585)
                     /locus_tag="IVZ90_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263000.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5605..5826)
                     /locus_tag="IVZ90_001964"
     CDS             complement(5605..5826)
                     /locus_tag="IVZ90_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263001.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(5936..6274)
                     /locus_tag="IVZ90_001965"
     CDS             complement(5936..6274)
                     /locus_tag="IVZ90_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263002.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6674..10315)
                     /locus_tag="IVZ90_001966"
     CDS             complement(6674..10315)
                     /locus_tag="IVZ90_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263003.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10318..12267)
                     /locus_tag="IVZ90_001967"
     CDS             complement(10318..12267)
                     /locus_tag="IVZ90_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8263004.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12309..13367)
                     /locus_tag="IVZ90_001968"
     CDS             complement(12309..13367)
                     /locus_tag="IVZ90_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8263005.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13486..13941)
                     /locus_tag="IVZ90_001969"
     CDS             complement(13486..13941)
                     /locus_tag="IVZ90_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263006.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIVKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14525..15328)
                     /locus_tag="IVZ90_001970"
     CDS             complement(14525..15328)
                     /locus_tag="IVZ90_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8263007.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         4568 a         3193 c         2233 g         5878 t
ORIGIN      
        1 aaaactctcg ctttttcttc atcagtaaag tttttataca actgattaca ataatgaggt
       61 ttttgaagtt tctgaatacc tatactaact tcaaatcctt cttcatgttg aattaggatt
      121 acttctgtgt ctctacctag aaatattcga tctaaaattt ttatgtttgg tttcattatt
      181 taacctccac tataattatt tcactcagta aggtttcact ttatgaaatt tctatagttc
      241 tcttttcaca aaacgtacca tgttctctaa gactgttttt agtctcaaat atacatgttt
      301 tttcttatcc tttccttata cactcaaaat caaagataaa acttaaagaa taaactctcc
      361 aataacaaaa ttattagtca gatccactgg tctcctgatc tgaaatttat tacttcttcc
      421 tgttgtcaaa tactgtctca aatcaacaga aagtttttga tcatcttctc cttttctagc
      481 aggagcaact tttttttcat acttcattaa ctctccactg gtcatctttc caatgatagt
      541 gtaattaaaa tcactttcaa aactttggta aatttttcca tctgtattga atatatcagc
      601 tgtttcaata tcttctttgt attcattgtc ttctcctgaa ccgattgtat caaatagaaa
      661 ggcaagacga attccatctt cttcaagcat tgttaatctt tttttaatta gcccaacggt
      721 ttcaatagat aacttatcga agccgtgaat cataacaata tcatcttttt ttgcttgtga
      781 tgtgatgaaa tcaaaggtat ttaaaaattg agcttccata acattggttt ctgctcttag
      841 atcacctaag ttatagtaaa actgtaattt gtcactttta ccgatttctt tcagtgtcgt
      901 tctttgatta aacatcctag agaagctgtc taatgctgtt ctcaatgtgt cttttaatac
      961 aataatacga tcgacttctt tttcagtacc agattttttg gcttttgttt cagcattagt
     1021 caaatagcta ataaaatccc caagaactgg cattgtatca ctactgttca ccaaaagttt
     1081 tgaacgttct tcataattaa tatcccaaag tttctgactt atataaaagt tacctaattc
     1141 ttttttcaat aaaactctag ttgcagaatc taatgttctg cctgctaaca aataaaaaat
     1201 ctgagttagt ttatcgagat tccgtctata taaatcacca atgtcctttt gattaccaaa
     1261 aagctccaaa gagttaagac cttcttcagc caaattaacc ttttctatta cactgtttaa
     1321 cgaaggtttg gcaacaaatg ttctaccagt tggatcacca aagtaatcaa atttatttaa
     1381 gacaatatga aaagttcgtt tatgatttgc cattgcatta ttagcaatca attgccccca
     1441 aagtgatgat gctgagattt tttcatcata gcctttaatt gtagcgtttt tgtgtgatgc
     1501 cactaaaact ttacttgtaa aagaatcatc cagtgccata aaagctacac cacttgcgta
     1561 gctagtagac atagtgccga tagcccatcc attcgcatca tctaaacctt tgcgaattgc
     1621 atgatcattt cctgcaaaat ccgaactcat agacgtatac tcatagacat tcccgattgg
     1681 ttcacttaaa agtctatcaa acagatcacg ttgatctcct ccatatgaca taagctttaa
     1741 tccatacatt ttttcatcat acaaccgttg gagtttaatt agctgttcgc tgattttatt
     1801 aggagaatcg gatactaata ggatttctag cttccaatca ataattttat ttgccatgct
     1861 gtctagtaaa gatgctgtat tcaaatccat aatccgcata acagttttgc taatttcagc
     1921 atctgaattt tttccttcta aattattttc ataactattc gttgatcccc caacattctc
     1981 gtgcattatt tcttgttgat ctttttgtga cactggctta tctgcttcaa ttagataggc
     2041 ttttacccct tctactctca cttcaggaat agctttaacc atccaaccaa atccataatt
     2101 tctattcatt ccataagtat tcactaattt cacaattgtt ccccaccgct tacctgtcct
     2161 aatgtaagat ggtgtaaatt gataaccaac taaagatgtt aaatcactaa caaaggtatt
     2221 attcactacc tttgaatttc tagttttttg ttctttttta gtttttctct tttttttgaa
     2281 atttaatagt tgtgatctac ccataatttc catttaatct ctcacctcta tttcagtgca
     2341 taaatatcag ataatatttc ttttgtttgt ttagctgaca actgagtgac actataataa
     2401 agtcctctac tcgtatagtt ttctaaacgt tccacacctt catccaatac catcttagat
     2461 tgagatttca agataagata ttggattact gttgttttca caccatcaat atattctgat
     2521 acaaatcgtt cttgctgatc tattatgtca aagactgctt tgttactcct tgcatttcgt
     2581 ttttgtattt tcatatttcg agtttgtctt tccgtattct gaggttgaga tgaagtgatt
     2641 ttgatttcag ctaaggaacg atctctggta ttttgatatt gtattgattt cgattcctga
     2701 gccataactt cttctggaaa agcggttagt gatgtagccc catctagatt gaacattaat
     2761 gctacactcc catctcgata tacgattgtt ccatcttgtt tcacttttaa aatctctgtg
     2821 cctcgaaatg gtttcttttt ccccatagat actcttgctg aattaactac tgatttgaac
     2881 agtctaatag aaccagaaat caatgaacca ctagatggat caataaacat atactggcta
     2941 attagaataa gaaatccaat tatcattaat aacgttggaa caaaaataaa tttcagttta
     3001 atcgtatcag aaactaagta aacaattgat ccaagaacaa cgactgcaac aactatagct
     3061 gatacaatta atctttttgc tgtaacagaa taggtgctat tgacatgctt ttcaagccat
     3121 tcaactctga caattgtttc agaaggtttt tgatgtaagt tatgtttaaa tccatagttt
     3181 gagttatctg ccatcagaaa tacttccttt cacgttcaac ttacggtaaa cacctacagt
     3241 tatcactatt gcaacaataa gcaagccaat taaagatatt gacttgctca ttgtctcgtt
     3301 cgtttgtggt agttcattta cctgatacac gagctgtgct tcctgtaagt gctgagaaga
     3361 ggtcatgttt tcttgattgt tttgaattac tggataaact attttttctt ctcgcataat
     3421 agccctcctt agttattatc agataagtga gcttgaattt tgtactgatt tccgtctgga
     3481 gttttcacaa ctgctttttc tccatcaata gaaatcactt cagccttagt tgttttccca
     3541 tcaatactga tgacaacatc tttcccaact ttgaactctg tagttggtgt tgcttcatag
     3601 ctactatttg ttgaagcaga gttcgtattt gtcccaatcg aattttccac tctcgaaata
     3661 gatgtttgag tttgagcctg tgcttgttga ttattatgac taataaagta ggacgatccc
     3721 aagatcgttc cgcaagccat tgcagcgaca gaaatgatag tagccatctt tttacttgta
     3781 aaatcacgtt ttgaacttga ttgattttct gtagcaactg ctaatgattt cgttttttct
     3841 gccatttgag ctagatcact tcgggaacga gataagtctt ctcttaaagc ttcactttgt
     3901 tctgccaact tttcgctatt taatttataa ccctctgcta aatctttgac ctctttcatt
     3961 cgatctttat attcttcgat agttttttgt tgctctacaa gttttgtttc taattctttc
     4021 gtgttgttag ctacctgctt ttcaacttct tctttgttcg caaaatattt gacaaccaca
     4081 tctgttgttt tctcttttgt atcatggaac tcagcttcat tttctgactt tgttttttcg
     4141 acaatctcta aagttttttc ttgcacgtct gttaatgatt tttcaatttc tggtttcaat
     4201 gaattgataa ttcgttccac cacaacttct tttgcttctt ggctttgttt caatgcatcg
     4261 tttttaatag attcaattgc ttcttggtac tgttgatcaa gactttcttc agttgctttt
     4321 ctatattcat cttcttgttg tttaacttct ggtaaatgct cagcatcata ccgttgctct
     4381 agtttcttta gttcagcatc caaataagcc tgtttcgcca tatcataatc tttacgaatg
     4441 atattggatt tttccatcac taaatcatca attttagatg cttctgcttc gtagctctct
     4501 ttagcaaccg atagtgtatt tgtaaactga ttttgaccat ttaatgaggt tgcttcctct
     4561 atccgcaaaa gctcatcagc taacatttgc tttcctttga ggatcaattg ctccttcgtc
     4621 ttatttaatt cactgttgat tttattatta gcaatagaga tgcgagcgtt catactctct
     4681 ttagctaaaa acagcaatgg tttcaactca ggtatggata gcatctcctc gtttaaatcg
     4741 acagggtcat taatctcatc taaaactaca ggttgaaatt tgtcagcaat gatctgttca
     4801 aaattttttt ctaaaaaatc agcatctaag tttttcggta catcactttc agatgtgatt
     4861 ttttcagctt cataaacata attttctttg tcactttggg attcgggttt ttcatcaatt
     4921 tttggttgtt ggtaggtttc tgtttgtatt tgtgtttctt ctatgcttac ttcttggttt
     4981 tctgcttcgg ttggtttgtt atcatccaca aaaagagctt ctttttcatc tggtacattt
     5041 tctgctacgg cttcaaattc aacttcttca gaagagttga aggtctcagc aattcgttct
     5101 aatccattag tagctacttc taattgctct actgcgctat agaagtctgt agcttcttca
     5161 acttctgatt caaacacatt aggatcatcc ggaataacta ataattgacc actactatcc
     5221 ataaaacgga cacctgctaa tagcttctct ttaaattttt ctggtagatc agctacataa
     5281 ttcaaacttg acttcaatag aacgacatac ttctcagaat cgagaatagc atcatcctca
     5341 tctataactc cctcactgtt gagcttttgt acatcttcaa ttaccatata atttttcttt
     5401 ggtaaatctt tggtcagcaa cgcatcgaat tctttccata cctttgcttt agcttttttt
     5461 gccttcgctt ctttttcttc ttttttgtac acttcgagta attgtggctt caaagagagt
     5521 gcaactacag gaactgtttc tgccacataa ttaatagatt cggtaatatc tttgaatttt
     5581 gccattattg taaaactcct tttttcagtc aatagttctt ttctgtttat ctagcatata
     5641 tacatatgct ttttcgaatt taataagcgt ttcaagtctg gctttttcaa tctttttact
     5701 cccatttcga taatcataga actggttaac attcatattc attatctttg ccagttcggt
     5761 acctttcaga ctgctcatta atacttcgtt ggcgtgagct aacatttttt ctctatcaaa
     5821 aaccatctta catttacttt cttccctcac ttctaaacta cgcttggagc gtagtttttt
     5881 ggtaaaaagt aaattgaaca aatcggcata ttatccaaat ttgttcaatt cacttttaac
     5941 ctaacagttc attttttgga aattttgaaa ctagatagat accaacaact ggaataagtg
     6001 ctgatatgat attagctttt tggttcaaat cattacctgt tttagtaccc attgtgtaaa
     6061 gcataacggt tgtaattaca ggaataataa agattgcaat aatcgcccca acatatttta
     6121 atccatctcc ccattttgca tcaccaaaaa atttcattgc agtgaatcct ggtttcaacg
     6181 aaaaaacgaa tgccaagaaa tacaaaacaa cttgtactaa agttttcata tcaggcgtca
     6241 ttccgctaac caaattttta atagaatcga acatataaaa acctcctttt taatattttt
     6301 aataagttaa ttatatcatc gacaaatctt atttgttcac ttgtcaatga tctaactaag
     6361 aaaaagacgt ctcgatattt acatctttat gttatcataa taacataaat tttcgatgat
     6421 taaaagagat tttccattaa attatttcac tttttttatt ggctaaaaat gctggtttat
     6481 caagtttttg tttagataat aattctgttc tttaccagca taagcccttt ttaattatac
     6541 aactttgaac agtaaatatt taattcattt atagacaatt taaaataatt tttttgcata
     6601 aatagaaatt ttccttgaca tcttcagtta gaaatcaaag taaaaagaag gagcaaatca
     6661 atgctccttc tttttactcg tcttcagact tattttcagt tgatttttga ttaccgtatt
     6721 ttacaatata gtccttgact gacatggtag atttcttcac tactttacca gttttggatg
     6781 ctcctgtagc agtagccttg gcaaccttag ccgttccttt acctatttta atagcttcgt
     6841 ttttagcttc ataggttatc cctgctagac taccttttgg agcttttgct aaagctttat
     6901 ctgctgaggt gtaaacattt ttaccagttt ttgatagata ttcaacggct gatttagctg
     6961 ttgatggttt cttggttgat tgattagatt tccctgaatt atctgaagta gatttagaat
     7021 tatttgttct attattttta tttgtcagtt caggttctct aattgattga ttgtctttcg
     7081 acattcctga tccaatattt gtttcatatt tatctgtttg attaacatta ggagtaggaa
     7141 tgaacttttt cttcacatct gattgttctt tatccgtttc ttttgttagc gattctctgt
     7201 ctttaagtac tgtgtcattc cgaatagcac tattatcaaa gttgctgtta agctctcttg
     7261 actgactttc accagatgct ccatctccta attcccgatg cactctgtct gacacgtcct
     7321 caccactcat ttcattcctc agttcttgat gagtttgatc taaagtaatc tcgtcttctt
     7381 cattctcagg aattgcaaca gcaattggcg tatgccgttg ttgttcgagt tctctatttc
     7441 gatctgccac aagcgcatct ctcgtctcaa tgatattccc atcctcatcg actaaatctc
     7501 cattcacatt atatccatcg ctgtgtttac caagtttatt accaaactca tcaacaagat
     7561 ttccatcttc atcaactaaa cgaccttttt cgtcaactaa acgtccgtta ctatcatgac
     7621 caaaatcacc atttagcaac ctgcgtccat tttcgtcaac aagatttcca tcttcatcga
     7681 ctaagcgacc tttttcatca attaaacgtc cttcgctatc cataccaata ttaccatctt
     7741 cactatcttc caatggttta ccattagtat ctataaggtt tccatcttca tcgaccaaac
     7801 gccctttttc gtcaataaag ttgcctttag catctttacc gtatttacta tcttttaata
     7861 tttcaccatt ttcatcgaca aagtttccgt tttcatcaac catacgtcct aaatgatcaa
     7921 tatattgttg ggagttatta cgtagttgat cttgacgaac taaatctttg aaagaggtat
     7981 tattgattgt ggaaccaact ccgccaccgt tgaatgacat tccttctggt gtttcaggaa
     8041 ttactttatc agcaatggac ttagcatcag cattgctatc tcccatattg ttcaaacgtt
     8101 cagccatttt ttgcgctcca actgctccaa gacctcctgc aaccatatct tttactgagc
     8161 tgttgccacc atcataatag ggatttttag ctccttggct tttcttgatt gctttagaaa
     8221 cgccgtccac aactcgcaca gcatcatttc tattaccaaa taataactta gcacgttcat
     8281 caaacccaaa cgctacttca agtggtaagc caataagcat attaatcaaa ttatttactt
     8341 tttcgccttt tttaaagact aaaagatatc ctacacctaa aatcatagca gatcgaacaa
     8401 tatttgctac gccatcgcca actaatgtat taggaatgaa tcccacagca gtatcaccaa
     8461 atgcacgaat aaaagtaatc agaaagatag gtaattgtag tccgatcaga gatgttgtaa
     8521 caacacctaa tgaaattgcc atcacacctg ctcctgatgc aagtgatcct gttagaacta
     8581 cttcccaaat ccgaataaat tcacgtttaa atgctttcac gatattcaca ttgaacatcg
     8641 cccaagcaca tgctaagtaa agaactgcta aaacgattga ttcagcgttc atcgctagcc
     8701 acgagccaaa cttacccata gctccatgac caactaagga tgctgtacgc cattctgttt
     8761 ttactgttga catattctta gcactgcctt gaattcctga aggagcgata ttatacgcat
     8821 aaaacttagc tcccgatgta tcaagtgccg attggagcaa taaagctacc gactgtgtgc
     8881 taaaagttgc attgttagca acgccccaag gttgagtagc atcaaagtta tgcgctgttc
     8941 cttttccatc aggcttgcga ttgtcgtctg tcacattttg atttgctgac caaatatatc
     9001 catttaaatt ggttggtttc ttaattgcac ttccttcttt tccatcccct aaattatagc
     9061 ctgtccaaac agcaggcgct cttcccatat aagcatcatt ttcggcgaca ttaacatcac
     9121 cggcataagt attaatattg aaagtatcat tcgatgacca ttcttttaat agagccatac
     9181 cgactgaatt agaatctaga tcattatttt gtctggcata actgctagta ttgatcgctc
     9241 ttatcattgc agatttttta gatggttcat aatctgtatc aacgaacttg atttcggatg
     9301 taggctttcc gtcaggtaca tcaccaccag tatcagatga tggagcaaga ttttgagtag
     9361 aagcccaaag tcttgtgtta atgatgtagt cagaaacaac atttgttggt atatctccat
     9421 ctttttttgc tgactggatg ccgttgatat aaactcccaa tagcgatcct gccactggta
     9481 agaatataaa caatacaaaa atacgaacaa tccatttttc aagtactggt gataatccct
     9541 tcgagttaac accatctttc gacaataatt tcattccttg ataacctagt attgctgtga
     9601 aaaagagcat agataagctt gctatcgctt taaataattc attatttaat cccaaattgt
     9661 taagcaagct agttaatgct ttagataccg gattagatac atccgatcca ccttctcccc
     9721 accccataag agaaaatata ttcatactca tgaaaaattt tccaaggagt tcatttatgg
     9781 attcagcaat attagaaagg attaaactaa ttgatccgat aaaactacca atcgtcctac
     9841 caatacttcc tagaatggat gatgcttgac tggcaatagc atttcctgag actgtttcaa
     9901 tatatccgta gtaactataa gttgctaaag tagatgctag ttgagtacct gcattacccc
     9961 ctgcaatcga attaccttgt ttagaaatgt cagaataagg gaaatcggca ctgataccac
    10021 cttgtcctaa caaattcatt tgtccagttt tagcggcact atcattaact tctttatctc
    10081 ccattaggat acgagcatat agtgtccaca ttccttgttc tgccggttta ctttccgcat
    10141 atgcaacgtt cttatttata actgtttctt gtttgctatc ttgattgtca ggagttggat
    10201 cattcattcc aaaaggagcg cctaaagtaa ctgctaatac tcctccaata actagacctc
    10261 ttacgaccca tcgttttatt ctaaggtttt gtagtaaata tttcttgaat ttcatagcta
    10321 cgcccctcca atcgaaaata caatcttgtt ttctttactt tcagcaacgg aaggctttgc
    10381 aataatttca gcattagaat caccttttcc ttcaaacgct ccggcttcac tcaacttaac
    10441 atcgaatatt tttgcgtttg gataagtaat tattgcttca gtaatcgctt cttcaaactc
    10501 agattcaact aaatgcaaga tattccttgc accccctgat ttagaatcct cttgtgaagc
    10561 catgtcaaaa actaagtgca tggagagatc gtctgcataa aaattttgac catatactct
    10621 ttctttccta ggtggaagac gtaaatctat atttaaatgt tttcgtacta attcagcact
    10681 cttcaacatt tcattataag caatttccag aagagtttgt tcggataaag cccaaaatgg
    10741 aactattcgt ccaaatcggt taataacttc aggctttaga ccttcaccaa tcaaggcttg
    10801 tttgatccct gatgcgactt ttcggtattc tctttttagt tcagtgctgt attcattaga
    10861 attttccttg tttgtaacac gagatagtga gctatatcgg tttaacgaat cgaacatctc
    10921 cgcacctgca tttgttgtcg caaataaaat tgtatttgac aatgaaacgg tgtcgtgaac
    10981 ttctcgatca tcccatcctc tttcagtaaa acttatccta ccttcatcta aaattgataa
    11041 aaaggcatcg agtgtttcta caccagcttt ctcatattca tcacacacaa taatcgcaga
    11101 aggatcatgc cttacatgag tacctataag tcgtctaaaa cgcattgcat catctttggt
    11161 tttgtagtcc ggcatattaa ctaagagaac tttattttca tcgccataaa gtcctcttgc
    11221 cacggcttta gccattgcag ttttaccgac ccctgttgat cctgtaaaca gagcagttgc
    11281 ttttggttgc tttcgacctt taaaacgatc aaatccaagc ttcatacgtt taaacaacat
    11341 tgttatagta taaactgcta acggttgtcc ttttatggac tttaattcct cttgaagttt
    11401 ttccatatca aaatcagaat ctagctgaat attatactta ccaaatattt gcaatagact
    11461 ttctcgatta taagcatttc cgtctactcg atcttttgca atcaaatcct ccattacagc
    11521 aattgattta gccggttcgg acaaatcctc acgataaagt ttgtttgctt gtacgatttc
    11581 ttgaagtaat tcatcaggaa tcctttgcga taatctaaaa gaagcctgag gatcatcttt
    11641 aacagaggat gtatttagcc attgtctcaa aatatcgacg acaacatcgc tttttagttc
    11701 attcaattct aacggttgta gccgtctccc taaggcatca tctcttttga tatagttttc
    11761 atattctttt cttgtagtag cggtaatcat ttgaagtgat tcatttgcta acggttcttt
    11821 tagcgaatca cccccaattt tattgccact cccaaaagag ctaacaactt tatgaaactc
    11881 gtcaatgaag aggacaaatt cattatcttt gtcctcagct tttaaaattc gctcatattc
    11941 tttcaatctt ggaattaatg tttctaaacg tgtttggagg acatcatctc ccccaacacg
    12001 caattgtccg attgataatt tatatacttg tactttcttt ccatcttgtc gctgtctttc
    12061 agcccattca cttgcaagtg ctgttttacc tgttccggct tcacctaaca agatagcttg
    12121 cggtgtttcc ggtctattca agatcagtga caactcatgg agttcgtcat cacgtcccac
    12181 taacttatgt tttggtggtt tcattggtgt gagagcatga ttaaattcgg tttgaatatt
    12241 cttaaaaaac tcggtactat tttccatctg tttgtgttca cttcctctaa aataagaata
    12301 atttacttct agtcagtcaa agttaggtac ttaaatgtcg tcttcatgtc tccaaaagaa
    12361 tctttgttaa agtcagcatt tttgtattga tcaatcaatt tttgtgtatc attgattgct
    12421 ttaatttcgg tctccattcg actgtctagt tgacgaatac tttcttgcga atctttatcg
    12481 tcaggatttt gttgtaaacg ttccagatac tcagcttttg tggcttcaat tttcttgatg
    12541 ttagtatttg aagcatttaa ctgatcttct agcttattta caacattatt cttacttgta
    12601 actgctacaa cttttgaata atcaatatcg ccatctttgg taagaaagct tccttgatag
    12661 acattagttg aatctgaata tggatttatt ctgaaattga tattatcagg agtattaaca
    12721 ggttttgctt ttttcccaaa agaaattact ccttcattat taacatcatt catagaatat
    12781 tgagaaagtg cttttgtaac tgaatctgct tcattcacat tgactttggt atcatcgtca
    12841 gctgtatcat caccagaaat tagtgatact tggtttgcaa taaaaacttg ataagctctt
    12901 tcctctaatt taccttttag gaataagaaa gcatccccat cagtacttaa catcccatag
    12961 ctcatttcaa gcttcttagg acgatctttt tcttttgttg ccatatataa gttatagttt
    13021 tttccttgcg tagataacaa ctttctagcg ttttgggagt aaccaagttt tactactgtt
    13081 acatctctat tttgatctgt ccacacatct gaaatagtga tttctgctcc tgatcgagca
    13141 aactccaatg aagaccctat aggtgttgta ttgttcacga gataataatt atagtttcct
    13201 aattgataaa tgtagctacc caagcctgtt aataaaaaaa gcactcctag aaaagttgca
    13261 agcataatgc gtaatccgtg acgtgttctc ttatccttat caccaagcaa ccctccaata
    13321 atactgtatt gatctttggt agatgttgaa ttgacgtttt tttccatcag tttgtctgcc
    13381 ccctttcata aaagagcaaa caatcagacg ttttttcggt aattctaggt tcgggatttt
    13441 gaatcatttc ggtatattct tccccattaa tatttaacac aaaaattatc ttcctttctt
    13501 cgttcctttc ttttgctctt tattttccaa ataatcaatg atttttgcta ttgtaccaag
    13561 atagtaattc ccctcagctt tgttttttcg gattttgcta accattggag taggtacaat
    13621 ctcttcatct ctcaattcac tgaaagattt tttacctaga tattttccaa taacagattt
    13681 taaaaaacgt tcaaaatctt tctcgtaagt tgttttcttg ccttttagtt catggttcat
    13741 attgtcaacg acttttgcgg tttcaataat tgttttagtt ctcagcttaa caatttcatc
    13801 tggaattttc aatcctcgta atttcatcgt tgtactaact ggtaacccat gctttactgc
    13861 atcagcaata ctaaaacttt ttagatcatc tagtaagctc tccatatagt taacaaattc
    13921 atgccaatta atagcattca ttgtctcacc ccttcatttt tttaaaatga ggttcgggcg
    13981 taacacattt atgttattat aatatcataa taacataaat aaatcaatca gcaaatttag
    14041 atatccctta aagtatcagc aattattcaa caagatattt attttagcta tttcagtcat
    14101 cataaaagac taattagcaa taaaataact aatattatcc atttatcaga cgaaaagaaa
    14161 aaacttatct aattactcta tattaacttt taacaaatag ttgacttgtc caaaaatgtt
    14221 accttcaatc tcacaaagat attaagatgt ttcctaccaa aactgatatc accggacttt
    14281 ttattctgtg ttgtccaaaa gtgttacctt tagtcgatca acaaattaaa aaaaattgtt
    14341 tttttcttga cttgtccaaa agtgttaccc atagttgtta aaatttgtat agattagcca
    14401 ttctttcgaa tattatttag actgaaactt gcttacaaaa aaacttcttg catttattgt
    14461 ccaaaagtgt tacccatact tttttaaaga agcaggttgt attaaaatta caacctgctt
    14521 cttctcaatc ttcttctaac caattatgca ttgaaatggt tggcaagttt gattctggct
    14581 cactaaaagt gaatctgagc cgatcaactc ttcttccttt ctttgcatag attttttcat
    14641 aatcaaaagt ttcaaatacc tgtattccgt tttcatcagg agctaaaaat tcttctttcg
    14701 cctgctctag tattcttttg tcaatatttg tcattctata acttgaaggt atacttaatg
    14761 ctcttctaaa atcttcaact ttgactaccc aataaccatt tcgccactct ttatttctgt
    14821 attgcatgag atgacgatac aattcttttg tgtagctact tcttagtttt gtaaaattat
    14881 caagttcaaa acgagtaaag tttttgccaa ttgagtttaa gataaattca aattcctcat
    14941 ttgccttaat tgtaactgtt tccttctgtt tatctatccg gaatttttga aaaagccaca
    15001 gctgttcata gacgtctgaa tcttcaaaac ggtaggttaa agtacctagt ttgtcactca
    15061 aagcttttaa cacagaataa aaactgtcct tttctcttct gtcgtatttg accaaataac
    15121 gaaagaggtt gaaatcaaaa gttacttctg ctgttccttg acgtttcatt tttgataaaa
    15181 tagcccaaaa cacatccatt tcaacaggct taaatcctcg caaagctgtt gtattcagac
    15241 cattgttata acgcacaatt gcatttgttt gttctgatac taaatctaca tcactatcaa
    15301 taatcttttc ggtaacttct tgagacattg attactctcc aatcttcttt ttgcgctcct
    15361 ttaatcatgt taatctggta ctaattaaag gaaacaattt tttctgcttg ttacctaaat
    15421 taaatcacaa ctaaaggtaa cagtcaacaa agatttgtta ctaatctaat tttctatttt
    15481 ttcgccttcc aaatcaagaa aaccgaaggt aacgtcttat cctagtgtga tacctcaaat
    15541 gtccactttt gttacctttg ctgtccaaaa gtgttaccct tactgtccac ttttgttacc
    15601 tttactgtcc aaaagtgtta cctttatacc cctttaatac ttactctccc aagagattaa
    15661 gcctccccta acagatatat taacagatat ttaacagata tattaacaga tatttaataa
    15721 aggaggatta ctcctctctt atttcaaaat cttaaaaata aaaaatctta aaaaaaacta
    15781 acactatcta ggagtttatt ttgcttgtgt aactttgaat aattgaaaaa aaggaaaagg
    15841 aggaagcttc agcttcccct tttatttata tt
//
LOCUS       DACDBG010000062        15585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDBG010000062 DACDBG010000000
VERSION     DACDBG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.062"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IVZ90_001971"
     CDS             complement(<1..257)
                     /locus_tag="IVZ90_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263008.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IVZ90_001972"
     CDS             complement(273..923)
                     /locus_tag="IVZ90_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263009.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IVZ90_001973"
     CDS             complement(934..2106)
                     /locus_tag="IVZ90_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8263010.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IVZ90_001974"
     CDS             complement(2125..2997)
                     /locus_tag="IVZ90_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8263011.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IVZ90_001975"
     CDS             complement(3019..3939)
                     /locus_tag="IVZ90_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263012.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IVZ90_001976"
     CDS             complement(3939..4208)
                     /locus_tag="IVZ90_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263013.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IVZ90_001977"
     CDS             complement(4205..4585)
                     /locus_tag="IVZ90_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8263014.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="IVZ90_001978"
     CDS             complement(5052..6515)
                     /locus_tag="IVZ90_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8263015.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="IVZ90_001979"
     CDS             complement(6579..9596)
                     /locus_tag="IVZ90_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8263016.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="IVZ90_001980"
     CDS             complement(9747..10586)
                     /locus_tag="IVZ90_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8263017.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="IVZ90_001981"
     CDS             10731..10976
                     /locus_tag="IVZ90_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263018.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="IVZ90_001982"
     CDS             11039..11461
                     /locus_tag="IVZ90_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263019.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="IVZ90_001983"
     CDS             complement(11555..11872)
                     /locus_tag="IVZ90_001983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263020.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="IVZ90_001984"
     CDS             complement(11899..12105)
                     /locus_tag="IVZ90_001984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263021.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="IVZ90_001985"
     CDS             complement(12086..12268)
                     /locus_tag="IVZ90_001985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263022.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="IVZ90_001986"
     CDS             complement(12282..12632)
                     /locus_tag="IVZ90_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263023.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="IVZ90_001987"
     CDS             complement(12658..12960)
                     /locus_tag="IVZ90_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263024.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="IVZ90_001988"
     CDS             complement(12963..13196)
                     /locus_tag="IVZ90_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263025.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="IVZ90_001989"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="IVZ90_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..>15585)
                     /locus_tag="IVZ90_001990"
     CDS             complement(14627..>15585)
                     /locus_tag="IVZ90_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263026.1"
                     /translation="NQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEI
                     EDMGFDQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIF
                     DDFDEKNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFV
                     PKDVLTWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETF
                     AEDLSKTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSD
                     QAEYSLLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
BASE COUNT         4091 a         3301 c         2447 g         5746 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttc
//
LOCUS       DACDBG010000063        14939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDBG010000063 DACDBG010000000
VERSION     DACDBG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.063"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..562
                     /locus_tag="IVZ90_001991"
     CDS             <1..562
                     /locus_tag="IVZ90_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8263027.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="IVZ90_001992"
     CDS             762..1076
                     /locus_tag="IVZ90_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8263028.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="IVZ90_001993"
     CDS             1097..1474
                     /locus_tag="IVZ90_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8263029.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="IVZ90_001994"
     CDS             1484..2257
                     /locus_tag="IVZ90_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263030.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="IVZ90_001995"
     CDS             2279..3682
                     /locus_tag="IVZ90_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8263031.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="IVZ90_001996"
     CDS             3864..5048
                     /locus_tag="IVZ90_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8263032.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="IVZ90_001997"
     CDS             5045..5335
                     /locus_tag="IVZ90_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263033.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="IVZ90_001998"
     CDS             5332..5553
                     /locus_tag="IVZ90_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263034.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="IVZ90_001999"
     CDS             5595..6374
                     /locus_tag="IVZ90_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8263035.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="IVZ90_002000"
     CDS             6441..6935
                     /locus_tag="IVZ90_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8263036.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="IVZ90_002001"
     CDS             6991..7629
                     /locus_tag="IVZ90_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263037.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="IVZ90_002002"
     CDS             7701..8096
                     /locus_tag="IVZ90_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263038.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="IVZ90_002003"
     CDS             8080..10533
                     /locus_tag="IVZ90_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263039.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="IVZ90_002004"
     CDS             10530..12557
                     /locus_tag="IVZ90_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263040.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="IVZ90_002005"
     CDS             12554..13576
                     /locus_tag="IVZ90_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8263041.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="IVZ90_002006"
     CDS             13593..14522
                     /locus_tag="IVZ90_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263042.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="IVZ90_002007"
     CDS             14767..14883
                     /locus_tag="IVZ90_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8263043.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5072 a         2485 c         2954 g         4428 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DACDBG010000064        14811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDBG010000064 DACDBG010000000
VERSION     DACDBG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.064"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(10..240)
                     /locus_tag="IVZ90_002008"
     CDS             complement(10..240)
                     /locus_tag="IVZ90_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263044.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(676..1242)
                     /locus_tag="IVZ90_002009"
     CDS             complement(676..1242)
                     /locus_tag="IVZ90_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8263045.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1242..2198)
                     /locus_tag="IVZ90_002010"
     CDS             complement(1242..2198)
                     /locus_tag="IVZ90_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8263046.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2198..2854)
                     /locus_tag="IVZ90_002011"
     CDS             complement(2198..2854)
                     /locus_tag="IVZ90_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8263047.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3224..5242
                     /locus_tag="IVZ90_002012"
     CDS             3224..5242
                     /locus_tag="IVZ90_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8263048.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5669..8083
                     /locus_tag="IVZ90_002013"
     CDS             5669..8083
                     /locus_tag="IVZ90_002013"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8263049.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8431..9984
                     /locus_tag="IVZ90_002014"
     CDS             8431..9984
                     /locus_tag="IVZ90_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8263050.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10365..12149)
                     /locus_tag="IVZ90_002015"
     CDS             complement(10365..12149)
                     /locus_tag="IVZ90_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263051.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12164..13885)
                     /locus_tag="IVZ90_002016"
     CDS             complement(12164..13885)
                     /locus_tag="IVZ90_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263052.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13985..14746
                     /locus_tag="IVZ90_002017"
     CDS             13985..14746
                     /locus_tag="IVZ90_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8263053.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4801 a         2749 c         2916 g         4345 t
ORIGIN      
        1 gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata tatatcgagt
       61 ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt gtttattttc
      121 ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt ttttactgct
      181 ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt cttccatcat
      241 aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg atatttgagg
      301 ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat gaatgacata
      361 caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat tcttttatat
      421 atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata aagaggttgt
      481 gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt
      541 ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga ataaagaggt
      601 tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat atcatttatg
      661 ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg gctggttctt
      721 ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt tggaacaaaa
      781 agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga cgacaaggat
      841 caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc gtgtagtctc
      901 tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga aaatccctgc
      961 tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt gtagaaacaa
     1021 tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat cgattgcttg
     1081 ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg ctagaaaaac
     1141 cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt gcaagatttc
     1201 ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct cacctttttc
     1261 cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc aattgcattc
     1321 aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca cggcgcatcc
     1381 cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc gcaaatgaca
     1441 ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc ccgcaacatt
     1501 ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc tgaatctaaa
     1561 atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa tccgttgatc
     1621 aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc taaataagtt
     1681 tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt ttgcaatcca
     1741 agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac tacttcatct
     1801 ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa attgactact
     1861 tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa attttggaaa
     1921 taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat ttctttctgg
     1981 aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc agatacactg
     2041 caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc aaatcctcca
     2101 tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta attccaagaa
     2161 tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc tttctgtaag
     2221 agttatctat ttgttttctt ttttctctat acactggata agtcaggcac agccaagaaa
     2281 gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga taaatgcggc
     2341 ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg cgtaaggttc
     2401 ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc atactgcctg
     2461 ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc aatgaaggac
     2521 gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt acaataaatc
     2581 cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat aaagccgcac
     2641 aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag agttggtagc
     2701 tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg aaaccagaaa
     2761 gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca agaaagaagc
     2821 taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga agcaatgact
     2881 tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca atgtatgatt
     2941 cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa gacatttttt
     3001 ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc cttttaaaca
     3061 agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac ggtaaattac
     3121 aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat taagagaagt
     3181 tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa acattgcaag
     3241 aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac gaggggcagc
     3301 aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac agccggttcc
     3361 tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag cggcatttat
     3421 cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat atgcatcagt
     3481 gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat ctcaagcacc
     3541 ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct acatggatac
     3601 gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac aatatccttc
     3661 ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat ttggcaacgg
     3721 ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg cgacagcttg
     3781 tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata atatcatcgc
     3841 aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg gtggagcgac
     3901 tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca attctggtaa
     3961 tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg attctgtttg
     4021 gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga acaacatcca
     4081 aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg aaaatggctc
     4141 ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta catcaaacgg
     4201 aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt ggaagatctc
     4261 aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca agaacaattt
     4321 cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg ctggagcaaa
     4381 tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa gttcttctaa
     4441 tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga gcgtctcaaa
     4501 taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa ataactttat
     4561 ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca cgtcaggtaa
     4621 tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca gcagttcgtc
     4681 tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa ataaaaacgg
     4741 catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca tttatccagg
     4801 acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt caactacttc
     4861 tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat ccactggtaa
     4921 tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca aacatcatat
     4981 cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct atccaggaca
     5041 gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag ttactggttc
     5101 gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca gtcatggaat
     5161 tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt atccaggtca
     5221 aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga attccacaaa
     5281 aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa gtagtaaaaa
     5341 gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt ttgaactcgc
     5401 ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc cgttacaacg
     5461 ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg tcctataaaa
     5521 agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa aacaaagcaa
     5581 aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa tagaaacaag
     5641 tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa ttgagaaaaa
     5701 atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc ctgaaaagcc
     5761 aaaattttac gcactggata tgtttcccta tccatccggt caagggctac atgtagggca
     5821 tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa
     5881 cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat atgcgctaga
     5941 tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc gacgccaaat
     6001 caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg atccggagta
     6061 ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag cttatgaagc
     6121 agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg aagaagtgat
     6181 cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc gccaatggat
     6241 gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag ttgattggcc
     6301 agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag caaatattga
     6361 atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac cagatactct
     6421 attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag aaattacgac
     6481 ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa aatcagatct
     6541 aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt atgcaatcaa
     6601 tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat cttcatacgg
     6661 cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat tcgctcaaac
     6721 tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag ctgcttacac
     6781 agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac aagaagcaat
     6841 cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg
     6901 cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc caatcatcca
     6961 ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac ggttgcctaa
     7021 gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata tcgaggagtg
     7081 ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc
     7141 acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata acaaaaaaga
     7201 actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca ttggtggagc
     7261 agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct tatacgatat
     7321 cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa tgatcttagg
     7381 cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg atgatgtggt
     7441 cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc cattagatgc
     7501 ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg atcgtgtatg
     7561 gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat tgaatgatgg
     7621 caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact atgaaaatct
     7681 tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct ataaagtgga
     7741 cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca
     7801 catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt acgcaccatg
     7861 gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt tccaagtaaa
     7921 cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg agctagaaaa
     7981 agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt
     8041 tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc tttgtcaata
     8101 aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt ctgtctcaga
     8161 ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt ttcatgttct
     8221 caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct gtaatcatca
     8281 aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa ttgagagtac
     8341 ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt ttgacgcata
     8401 atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat tatcgaagta
     8461 ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc
     8521 aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt atcgttccta
     8581 tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt aaataatttg
     8641 tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt ttttcttcta
     8701 cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga
     8761 gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct ttattttgag
     8821 aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt attgctgaga
     8881 tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg aatgaacaca
     8941 ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt
     9001 ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat tcttttagct
     9061 ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat aaatgctgct
     9121 tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa tgaagggttg
     9181 ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga tctcaaactt
     9241 gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg ggtatttgtg
     9301 ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg gctgattcct
     9361 gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg gtttatctat
     9421 tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac atggcaaacg
     9481 tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt ttatggcaac
     9541 gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt gtacatttac
     9601 actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat caatcagcca
     9661 gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg tgaaagtttg
     9721 ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat tccggaaaac
     9781 tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca aataagcgcg
     9841 aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc agggaaaatg
     9901 tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt tcagcaacta
     9961 tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt cagcaccgag
    10021 tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt aactcgtgat
    10081 tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt attttacaag
    10141 atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa aaagggattg
    10201 tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca aaaagtatca
    10261 ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa gctatcgatc
    10321 atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc tatctgaaat
    10381 tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga atgtgttcct
    10441 ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc aatcgttgac
    10501 aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat tgcttgctga
    10561 atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat aacgacagga
    10621 ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg agataacgtg
    10681 ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat aaagtgatca
    10741 cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat tttcccgtag
    10801 gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac catcccaaaa
    10861 gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt
    10921 cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt tcctgcacca
    10981 gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac ttgattcatc
    11041 aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt tcctttcggt
    11101 tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc taatatctca
    11161 aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata agaagcggta
    11221 gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa gccgatcgtc
    11281 acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa ggcgagttga
    11341 ttaatgaaac gaatggccgg atagatagca cagttcataa attgagcacg tttaaaagcg
    11401 ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg ctgattgaat
    11461 gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa aacacccagt
    11521 tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt tgtaacaatt
    11581 gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat ataaaacagc
    11641 atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat acctgtcaat
    11701 aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc acctacttga
    11761 tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc ttttcgcacc
    11821 ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa tgaagtgatg
    11881 ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc ttgtttgata
    11941 tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag atcatcgatc
    12001 ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa actgcataga
    12061 aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg tatcaaagca
    12121 cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt ccttgtgatt
    12181 ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta ccttgtccaa
    12241 cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta ctgaggcgtt
    12301 gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct cttaatgctg
    12361 catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc tctgctggtt
    12421 tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa ttagaaccgc
    12481 cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct tctaattgag
    12541 cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc atctgcaagt
    12601 tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg atgtgggagc
    12661 ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact tttcctatcg
    12721 tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat ccggtcccgc
    12781 caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct agaactggat
    12841 cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt gtctgatcaa
    12901 aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct gcttgcagta
    12961 tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat accatagctg
    13021 cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc acgacaccga
    13081 tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa gcataaacga
    13141 ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc actttgatca
    13201 tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat gattgattat
    13261 caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg gtgatccgat
    13321 ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt cgataaacaa
    13381 tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga gaataacgaa
    13441 acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt tggcaaaata
    13501 aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa agagatccag
    13561 aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa tatttctccc
    13621 gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag gcaactgcag
    13681 cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt ataactgagg
    13741 cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt acacctaatg
    13801 taccggcgat ttctaaacag atcatcacaa aggtaaggag agcgagtcct ttgtttttct
    13861 gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac ttttcgattc
    13921 tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa agaggtgaga
    13981 aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt tgccaaaatc
    14041 aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag aagagaatga
    14101 ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt tttatagaaa
    14161 attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga cggagttata
    14221 tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga tgaaacaaaa
    14281 acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata caaacgagga
    14341 agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt ttgaagatat
    14401 tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga gtatgccagc
    14461 ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac atttatttac
    14521 tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct tattgaaatc
    14581 cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa tgtatgaaaa
    14641 aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg atgaaaacaa
    14701 tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa tatgtgttta
    14761 agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg t
//
LOCUS       DACDBG010000065        14749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDBG010000065 DACDBG010000000
VERSION     DACDBG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.065"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(177..1757)
                     /locus_tag="IVZ90_002018"
     CDS             complement(177..1757)
                     /locus_tag="IVZ90_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8263054.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2158..3534)
                     /locus_tag="IVZ90_002019"
     CDS             complement(2158..3534)
                     /locus_tag="IVZ90_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8263055.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3662..4780)
                     /locus_tag="IVZ90_002020"
     CDS             complement(3662..4780)
                     /locus_tag="IVZ90_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8263056.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4922..5356
                     /locus_tag="IVZ90_002021"
     CDS             4922..5356
                     /locus_tag="IVZ90_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263057.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5434..5967)
                     /locus_tag="IVZ90_002022"
     CDS             complement(5434..5967)
                     /locus_tag="IVZ90_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8263058.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6061..7239)
                     /gene="mutY"
                     /locus_tag="IVZ90_002023"
     CDS             complement(6061..7239)
                     /gene="mutY"
                     /locus_tag="IVZ90_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8263059.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7232..8029)
                     /gene="recX"
                     /locus_tag="IVZ90_002024"
     CDS             complement(7232..8029)
                     /gene="recX"
                     /locus_tag="IVZ90_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8263060.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8136..9518
                     /gene="rlmD"
                     /locus_tag="IVZ90_002025"
     CDS             8136..9518
                     /gene="rlmD"
                     /locus_tag="IVZ90_002025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8263061.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9692..10270)
                     /locus_tag="IVZ90_002026"
     CDS             complement(9692..10270)
                     /locus_tag="IVZ90_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8263062.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10592..11062
                     /locus_tag="IVZ90_002027"
     CDS             10592..11062
                     /locus_tag="IVZ90_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8263063.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11170..12396)
                     /locus_tag="IVZ90_002028"
     CDS             complement(11170..12396)
                     /locus_tag="IVZ90_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8263064.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13150..13350)
                     /locus_tag="IVZ90_002029"
     CDS             complement(13150..13350)
                     /locus_tag="IVZ90_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8263065.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13484..14092)
                     /locus_tag="IVZ90_002030"
     CDS             complement(13484..14092)
                     /locus_tag="IVZ90_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8263066.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14177..14647)
                     /locus_tag="IVZ90_002031"
     CDS             complement(14177..14647)
                     /locus_tag="IVZ90_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8263067.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         4411 a         2974 c         2435 g         4929 t
ORIGIN      
        1 aattaactag caccacgggt atttttatat ttttttgatt agacatttct atgattttaa
       61 tatagtaaaa agagccgtca cggaaaatcc gtcacggctc taaacaaaat atgagaggct
      121 gtttttgttt ggctattctt attacttgat gcagggcagc ttgtgtcaag acttctttac
      181 atcaaacttt ttaattttac attaggatat ttatccgcaa accagcgttc tgcaaattgg
      241 ttctcgaata agaacaatgg ttggtcgaaa cggtcacgag ccaagatatt gcgacttgaa
      301 ctcatcttct catccaaatc ttctggatcg atccagcgag cgattttgtg tcccattgga
      361 gacatgatta cttcggcgtt atactcattt aacatacgat attggaatac ttcgaattgt
      421 agctgaccaa cagctccaat gatatattct tccgttagat atgttttata aagctgaatc
      481 gctccttctt gaactaattg atagattcct ttgtggaagg atttttgttt catgacattt
      541 tttgcagtca ctttcataaa cagctctgga gtaaatgaag ggagttcttc ataagcaact
      601 tttagttttc cttcgaacaa tgtatcccca atctgataat tgcctgtatc atatacaccg
      661 atgatatcgc ctgctactgc tttttcgacg ttttctctcg aatctgccat aaattgtgtg
      721 acattgctta atttcagttt tttatttgta cgttccaacc agacatccat tcctcgttcg
      781 aatgttccag aacagattcg gacaaaagcg attcggtcac gatgggctgg attcatattt
      841 gcttggatct tgaatacgaa acctgaaaat tcttcctcat aaggacttac catctgatca
      901 tcttgtgttt tgtgtccata aggagctggt gccaattcaa cgaaagtctc taaaaaagtc
      961 tgtacaccga agttagtcaa tgcagaacca aaaaatactg gtgtctgatc gccacgagca
     1021 attttttctt gattaaatgc atctccagcc tctttgatta gttctacttc ttctaagact
     1081 tgttcatata aggaattttc agttagagga tgattttcgg gaagcagtcc atcttcatcc
     1141 aattctacta atcgttcatc ctgataattt tccggacgat aaaactctac acgattatgg
     1201 tagatatcat aaagaccttc tagcccttta cccataccga ttggccaatt catcggataa
     1261 gattcgatat ctaatagctc ttctaattct tctaacaatt ccaatggttc acgaccatca
     1321 cgatctagtt tgttgataaa agtaaatatc gggatgccac gtttttttac aacttggaat
     1381 aatttctttg tctgtgcttc gatccctttt gcactatcga ttaccatgac cgcactatcc
     1441 acggccatca aagtacgata agtatcttca gaaaaatctt catgtcctgg tgtatccaag
     1501 atatttactc gtttcccttg ataatcaaac tgcataacag aactagtaac agaaatccca
     1561 cgttgtttct cgatttccat ccagtctgat ttggcaaaat tacctgtttt tttgcctttt
     1621 acggtcccgg cttgacggat agccccacca aacaatagta attgttctgt aatcgttgtt
     1681 ttcccagcat ccggatggga aatgatcgca aaagttctgc gattgtccac ttgttgcttt
     1741 aattctggat tattcataac ctaactccat ttcttccatt caattattta tattttctct
     1801 aggcacagat tgtccgatct cttaaagaaa gacaatttca tatagggctg tgacaaaagc
     1861 cgtgtagcag tctctttttc cgagtaaacg gcgttctaga gacagcctat cagcaataag
     1921 cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg ctgctcttat tacttaatgc
     1981 aggatagctt tttcacaacc tttgtcaact actacaactg aatcagtata gcatggattc
     2041 atcgaatgaa aaagccacca aaccataaga gtcaaatatt tttttgtcag ctgactaaaa
     2101 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
     2161 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
     2221 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
     2281 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
     2341 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
     2401 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
     2461 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
     2521 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
     2581 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
     2641 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
     2701 aaaaccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
     2761 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
     2821 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
     2881 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
     2941 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
     3001 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
     3061 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
     3121 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
     3181 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
     3241 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
     3301 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
     3361 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
     3421 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
     3481 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
     3541 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
     3601 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
     3661 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
     3721 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
     3781 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
     3841 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
     3901 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
     3961 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
     4021 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
     4081 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
     4141 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
     4201 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
     4261 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
     4321 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
     4381 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
     4441 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
     4501 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
     4561 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
     4621 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
     4681 aaataaagtt tatttctgat gaaatcatga tcaatgcagc tatgatcaat aattcaagcg
     4741 accaaaacat tagtttagac tgtctaagtt tttcaaacaa tagatttcct cctttctttc
     4801 ttactagtat atattatatt cgttaaaaat tcttttattc tttttttatt tttgaaaact
     4861 tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag gtggctagac
     4921 catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg ctgtacggat
     4981 caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga
     5041 tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt
     5101 acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga ttctttctga
     5161 atacagacac caagggttag gaaaaatact gattgaagaa gcagaaaact ttgctaagaa
     5221 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
     5281 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
     5341 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
     5401 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
     5461 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
     5521 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
     5581 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
     5641 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
     5701 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
     5761 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
     5821 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
     5881 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
     5941 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
     6001 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
     6061 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
     6121 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
     6181 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
     6241 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
     6301 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
     6361 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
     6421 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
     6481 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
     6541 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
     6601 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
     6661 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
     6721 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
     6781 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
     6841 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
     6901 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
     6961 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
     7021 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
     7081 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
     7141 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
     7201 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
     7261 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
     7321 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
     7381 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
     7441 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
     7501 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
     7561 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
     7621 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
     7681 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
     7741 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
     7801 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
     7861 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
     7921 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
     7981 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
     8041 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
     8101 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
     8161 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
     8221 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
     8281 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
     8341 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
     8401 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
     8461 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
     8521 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
     8581 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
     8641 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
     8701 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
     8761 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
     8821 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
     8881 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
     8941 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
     9001 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
     9061 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
     9121 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
     9181 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
     9241 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
     9301 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
     9361 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
     9421 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
     9481 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
     9541 ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg ttttatgaat
     9601 actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa aagggttgtg
     9661 acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct atcacttgat
     9721 aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta tatccttttc
     9781 gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt gctttgtcgt
     9841 ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga ttccaagttg
     9901 gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa ttccaagtaa
     9961 tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc tgttcatcta
    10021 aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat gtatttttgg
    10081 tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt aagtttcctg
    10141 ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca tgcgcaaaac
    10201 ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat aacgtactaa
    10261 tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct ttattgtatt
    10321 aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga
    10381 tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag aatcaaatca
    10441 tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt aataacaaca
    10501 attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga gccttcatgg
    10561 taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata ttcgtttttg
    10621 ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta gcggaaatcc
    10681 cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct ggatatttgg
    10741 gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt tacttaatca
    10801 caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt tcatttttaa
    10861 ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag caatcgatat
    10921 tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc atcatcgcct
    10981 gacaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc tattgaatta
    11041 ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta acacatagaa
    11101 aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta ttaaccaaac
    11161 tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat aaccataacc
    11221 attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg gtgtatagta
    11281 ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact gatcatatgt
    11341 gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat caccgtttgt
    11401 cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat ttttcaaatt
    11461 aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt ggatcgaaag
    11521 aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc cttcgccttg
    11581 caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta catcaagcat
    11641 gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa tttttctata
    11701 attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta cttcccgctg
    11761 acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag aataggctaa
    11821 acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat ccaaagtaaa
    11881 gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt ccatgccatc
    11941 caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa ttgaatccat
    12001 ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg ttccataacc
    12061 tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt tttgtttcgg
    12121 attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc ctaatccacc
    12181 ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc ttactctctc
    12241 agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg tttggctcgc
    12301 ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa cgaatacccc
    12361 taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt tcacattttc
    12421 taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc ataagaaaac
    12481 tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa attcttaact
    12541 atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc aatcgtttta
    12601 tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc aaataatcgt
    12661 gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc taccgttcag
    12721 attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt aaacccatct
    12781 tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa ctagtgattg
    12841 cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac gattttcttt
    12901 taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac aaatggtctt
    12961 ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa gaaaatgttc
    13021 cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt agccaaaaaa
    13081 tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt gtctttattc
    13141 aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac cttcaacaat
    13201 cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt gaattgctga
    13261 gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc ctttttcatt
    13321 gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa agttcttgca
    13381 agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt tcgctctttt
    13441 attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt gctgcttcga
    13501 aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata taatctggtc
    13561 ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct agaattggtg
    13621 tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct aattttccat
    13681 tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct tttttgaatt
    13741 cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt atttctcctg
    13801 taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg ttagcatgtc
    13861 cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc atatcagata
    13921 ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct gaatttaaat
    13981 tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct tcgtcaatat
    14041 aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc ataaaaaaat
    14101 tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag agaaggaaat
    14161 tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct tcaaaaacct
    14221 cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata acaataattt
    14281 gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac aataccattg
    14341 cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg cattcaataa
    14401 gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc gttacaaaag
    14461 caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt aaatcaaaat
    14521 attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca ccaaaaaaga
    14581 gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac tgtcgatact
    14641 gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta tatttcaata
    14701 actttcatct ttatttaaaa aaattaattt tttttgatcc tttgccttt
//
LOCUS       DACDBG010000066        14199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDBG010000066 DACDBG010000000
VERSION     DACDBG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.066"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(13..318)
                     /locus_tag="IVZ90_002032"
     CDS             complement(13..318)
                     /locus_tag="IVZ90_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263068.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            complement(500..931)
                     /locus_tag="IVZ90_002033"
     CDS             complement(500..931)
                     /locus_tag="IVZ90_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263069.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            complement(946..1242)
                     /locus_tag="IVZ90_002034"
     CDS             complement(946..1242)
                     /locus_tag="IVZ90_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263070.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            complement(1319..1738)
                     /gene="ssb"
                     /locus_tag="IVZ90_002035"
     CDS             complement(1319..1738)
                     /gene="ssb"
                     /locus_tag="IVZ90_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8263071.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            complement(1793..2134)
                     /locus_tag="IVZ90_002036"
     CDS             complement(1793..2134)
                     /locus_tag="IVZ90_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263072.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            complement(2206..2472)
                     /locus_tag="IVZ90_002037"
     CDS             complement(2206..2472)
                     /locus_tag="IVZ90_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263073.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            complement(2959..3870)
                     /locus_tag="IVZ90_002038"
     CDS             complement(2959..3870)
                     /locus_tag="IVZ90_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263074.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            complement(4813..5235)
                     /locus_tag="IVZ90_002039"
     CDS             complement(4813..5235)
                     /locus_tag="IVZ90_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8263075.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(5225..7399)
                     /locus_tag="IVZ90_002040"
     CDS             complement(5225..7399)
                     /locus_tag="IVZ90_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8263076.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(7415..9757)
                     /locus_tag="IVZ90_002041"
     CDS             complement(7415..9757)
                     /locus_tag="IVZ90_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263077.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(9779..12322)
                     /locus_tag="IVZ90_002042"
     CDS             complement(9779..12322)
                     /locus_tag="IVZ90_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8263078.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(12332..12730)
                     /locus_tag="IVZ90_002043"
     CDS             complement(12332..12730)
                     /locus_tag="IVZ90_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263079.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(12811..13095)
                     /locus_tag="IVZ90_002044"
     CDS             complement(12811..13095)
                     /locus_tag="IVZ90_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263080.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(13119..14117)
                     /locus_tag="IVZ90_002045"
     CDS             complement(13119..14117)
                     /locus_tag="IVZ90_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263081.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         3974 a         3025 c         2208 g         4992 t
ORIGIN      
        1 ccgctaaagt atttaaaaat atcgtatatt cagttctgct agttgctcta tttcttctgt
       61 aaaacgtttt gtcgcattcc cactctcatc tgaatcaatc aaacatgttt taccaactct
      121 tgacagatac accgctagtc ttccatctcg aatttcatga ccaacattct tacttttcga
      181 taatgttctt tgcattcgca atcttatatc tttcgaatat aagtcggtta tagacccgta
      241 cattcgcaag ttcgtcagtt taattaaaaa cagtctcacc ctgagtaatt tatagcgtcc
      301 cggcatatcc aacttcatat gattccacac ttagaaattg ttctgccgcc tttacctagc
      361 ttcatacaat aacttcctag tagactttat tgcatgtagg agtatcagac cagttgattc
      421 agcagacatg atagctttca ttccaactgt caaataggtt agttgtgatt aatcttaaga
      481 ttaatcctcc cgtttcgtat tacatactta tggggaaact tgtcgcacgt tgatagccaa
      541 tcaaatgaca acgattataa agataatcgc cattcttcat tttcttttgt ttccagccag
      601 ttggcttaat gtataatcgg tctcgttctt ctgttggcat cctgctttta tgcaaaagtg
      661 cacttgctac acctacccga tttaaacgat ccaaatctga aaaaaattgc ctggttccat
      721 tttctaacga aaaatcttct tcagaaaaac tcggtttatt ttgattcact atttctactt
      781 gtgtatttag cggttgttct tcatcttgtt ttgttattgc ttgttttatt tccgataagg
      841 gagctttttg aacgtcaatt gcttcacaac ctgtcccaac aaaatcaatc aatgctagca
      901 caataaaaat acttcgtttt aatcttttca ttttttctct cctctttaat cattgttttc
      961 ttgttcatat cgattgaaca attgaatcgc atttatgggt tcttctcctg tttgtttcgc
     1021 aatcaattta gacaaaattt tttccaacca ttttttgtct ccgacataat ctaaaaagtt
     1081 gcccatcgtt gttaatacca attcatcact tgcaaaatct accaagcgaa cgctttgata
     1141 aggaggtaaa cttgcaacat aattaataat attttcatct gtataatcaa acgagcccaa
     1201 atattgcatg gttcgttgat cgtaactgtc aatgattttc ataaaaaatc gcctcatctc
     1261 tatttatttt ttgttctata cctgaagaaa tcaggtatag aacaaaatta tttttcactt
     1321 aaaagggcaa ataactatct gaaatttgaa tctcatttcc tggaattgaa gaattatttt
     1381 gattattcgc cattgatttt gaccgttgct cattgatttc tttactctct aacaaataga
     1441 aatctgtcac tagaacttcc gtaacataca tggtctgtcc ttgctgattc tgatagctcc
     1501 tcgtttgaag acgtccatta atgccaatca aacttccttt tccagtatat tttgctaaag
     1561 tttccgcagc tttccgccaa atgacacagc ggatataatc tgcttcacgc tcgccgtttg
     1621 cattcgtata atttcgatta acagctaaaa taaatgtgcc tgtcgcattg ccttttggtg
     1681 tgtattttaa atccacttct tttgttaacc gtcctactaa attcactgaa ttcatcatga
     1741 ttttttctct ccattcttct atttattatt tttgaaaggg gaaactatcc ccttaataat
     1801 aactactttc ataaacagct tgatttttat atctatgaaa cgtgtcccaa ctacgataac
     1861 cacataacat atcaggatag gtttctggat caattcgttt ttttacaata ctgtctttag
     1921 tttttgctaa aggatactct ataaacttgt ctttcattgg aacaactttt ccactcatgg
     1981 acgaataatg ctcgaccgtt ttcttttgaa taggctgaaa ccatgctgtt ttttctgtta
     2041 acttcacaat ttgccaaaaa gtcacgttcg tttgttcgta gccccaacta ctataaaaaa
     2101 tgtctcctac ttgataaggt gtgttttttg ccattttctt tttccttctt tctttatatt
     2161 tttaccgtac aaagagaatc attctctttg cacgtcatct gacttttata agaagttttc
     2221 aagcttcccg ttgttaattt cccactcata ccattcagtg aaatcttgcg gaaaaccatt
     2281 cactgcatgt ttttcttgtt cttttacttc ttgttcgttc attgggtcta aggctgtagg
     2341 tttgtttatc ggtcttgcat aacaaccaat ataggtttca aaatctcctt caaagctccc
     2401 ctcaataatc aaacctcttt cttgcaactc ttttcttgcc tgttctttca gttgctcgtt
     2461 tttagttgtc atggttgttt cttcctttct tgaatagaat gttttatttg attttttcgc
     2521 cacccacccc caccttttcg gtagtgtgtc gggggcacgg gggtttgggg gtgcaacccc
     2581 caaggtcttc attcggctcg tttttagacg aaggcctgct tgtttgattt tgggtttcag
     2641 ttgtcaagga tcaaagtgga cttctgttta gaggcccttt tcgtatagcg gtcgttcggc
     2701 ataaccggct cgaaaacatg ttctgacgat tgccaagggc gcgcagcgtg cattttaccc
     2761 ttggcaattg gcaggttatg ttttaggtta tactcgccga atgactgcta tacgattagg
     2821 gactctcgaa cgtccacttc tttgacaacg gataaatcaa acaagttagt ccttcctaaa
     2881 aacgagccgg ttcagacgct tgcgtctgaa cggtgccagc aaactggcac aaaaaaagag
     2941 cgaagcgaac accctctttt acaatacttc aggcgtaaac ttcacttttg gtttttcctc
     3001 attttcagtt gattcttcaa ataaattcaa acgactttga acagcgccat tcggataaat
     3061 tttttctcgt tctactgtcc gccaattgac tgctggatca ttctctaata atgcttttaa
     3121 tccctcttca aactctaaag ctgaaaaaat atgcaaccgc tcaaacaatt cttgttgact
     3181 cgcatctttt tcagtttcgc gcttgaaaaa aatacgctta aacgcatgaa gttcttcttg
     3241 aattgcgcca ctaggtgtca cataaattaa atgtgattca ggacgtgttc gcaactcacg
     3301 aatatatttc ggaaaaatcg cttcacggta gcgacgacgt gttttcaacg ttagctcaat
     3361 ttcaaaggac caattgatct catgattttc tttgtctcga aaacgaaaaa taaaatctgg
     3421 cacaaaaaat tttcttcctt ttgtcttagg atttaaatcc ttcgtttcat agacggtacg
     3481 tctttctgaa ataatccgtt ttaaatgtgg gtggtctttt gccaatgcca ttcctttaat
     3541 caaataattg ttaatttgta aatggtgatt caaattatat tcaggaactt tcggcaacgt
     3601 ataataccca cctatgcttt gatgtccctc tttcgtcaaa taataataca tttctgttcc
     3661 atattttttt cgatcgatta actgtttttc atataatctc actaaagcat tcgttactcc
     3721 ttgtttacta attgatctgt caaaattcaa tagtttgtat agctgttttc tagtaaccac
     3781 tctcaattca tgaagcaaac atagaatcgc taaagtacta ctattgcgag tagaataatc
     3841 aataaaatag ccatgcaata aatacttcat atttcatcac cttcaattta ataatgcttt
     3901 cttgcaagtt tctgttcgaa caaatcttcc tgattcctct gcaactgctt tactcccctc
     3961 tcttgtaagt ttgtgttcga acaagtcttc aagtttcctc tacacttgtt ttactccaac
     4021 tctctcgcaa gtttctgttc gaacaagtct tcaagtttcc tctacacttg ttttactccg
     4081 gctctctcgc aagtttctgt tcgaacaagt cttcaagttt cctctacact tgttttaccc
     4141 ccactctctc gcaagtttct gttctggaca gccccaagaa cactggggcg ttggccgtgg
     4201 ggttgattct ccccctcttt caaaagaggc tgaaagaggg ggttaggggg gagttagttt
     4261 tgagacccca agactggggt aggggttgat tttttccctt ttaaatttta tgctactact
     4321 atttttagta gtgtattctt tatctgttgg ctgtcggcgc ttggtatgtt attcggagac
     4381 ttcccgctta ggtttttgac cattttctct cataagtggc ggtttttcca cgttgagaga
     4441 aaatggtcaa aaacctggaa gtcggagaat gcctttcata ccaagcgccg acagccctcc
     4501 ccccacttat tacatataac ctttaaataa tctctaaata accctcaaat aatccttact
     4561 aatactgaaa cgtaaaaaaa ataaacggat ttcaaacaaa atacgtttat tttttctatt
     4621 gattttagct caaaatcgat tcaattcgag tgaaatttgc ttcaattttc aattggtttt
     4681 tgttcaaatt ttgaaacatt tttgctcaaa aaatgagtga atttgtttca aaagaaaaac
     4741 aaatacgatc gaaaaaaatt tgaactcata acaatttttt gtttttttca acgtattctc
     4801 tttcaatttt cactaaaaat taggaagaaa ttgattcaat gtcttttcaa aaaagaatgt
     4861 ttttctctca atttttttga cgtttctatc atgaatcacg agaaaacttg ggacactttt
     4921 aacatggtaa cgttgtaaaa aagtttgaag ttcttcttgt cttggttctt ttcttgtttc
     4981 aattcgatat acattcctag agaatttttt gtcgtatctt tccaagaaag attcaacttc
     5041 ttggcatttt ttacaatcat ttcttgctag atacacaaaa aatgattgat tcgttttttg
     5101 aagttctagt acttcttcta aattactatt gattagtttt ccatttgatt cttcgttagt
     5161 ttttatcagt ttaaaacaac caaataagag gaaactaaca ataagtatcc taacaataac
     5221 tttcttattt cgcatattgt ttctccaaat ttggtttccg aatatagacg actcgcggtg
     5281 gttcctggac gtcttcagct gttcgataca ccacaaattc gcctgttctc aatgctcgaa
     5341 tgcgattcgg attgaattta aagcggtcta cgtctcgttt cgtgcctctt tctcctgtcc
     5401 aatcaacctt tcccatatca gaataacctg cttcttgtgt cgtcatttcc gttaattcaa
     5461 tgtcttcttc tgtccccatt aaatctgccc aataatcaat ttctgtcggg tcatttgtct
     5521 tcccaataaa gaacgtattc acattcccaa tcaactgttt tcgaaggaca ggatcaattc
     5581 tatcaatgtc cgcaagcgtt tgaggggcat acgccgcatg caccccagcg cctcgtccct
     5641 tattgactaa gtctataagg ttatcattta gataagaagc tggttcgtca aatattacta
     5701 ggactttcga gcgattggct gtttctcctg cttgtttata cacttctgac gcaaagaaat
     5761 taatatctcc cacaagcatt tgagccaatg tccgaataaa ctcactataa atcaatccgt
     5821 ttaacgagat ataaacaatc tttccttctt taacaacttc ttgaatatct aattctcttt
     5881 ttcctccaga agtatcaaag agttcaccaa gctcactatc caataacata ttgatattgg
     5941 tctgtaaatt ctcagaagtc gcatacaaat aaaacgggtt cttcttatgt tcgtaacgaa
     6001 caaacaaccg tttaaacaac gtttgctcga tttctgtcat gagagaaatg ttttctttta
     6061 ataatagata atacgtttct aagtcaacgg ttttacgatc atgttcaacg attcgctctt
     6121 cacttaactg ttccacatcg gcaaaatcac gttctgtttt gtccatcgct tcattctttt
     6181 tcgttttttt cttcgtggtt ttcttctttt ctacgaccac tttttcttta aataaatctg
     6241 gatttttcaa ctgaatacga tccgcaaaca aatgtaagac gtttcgtggc aataaataat
     6301 gttgtaacca aactagattt ctttcaattt tatggctctt ttggaactcg tctaaaagct
     6361 gaatcgttac ctgtaataac gctttggctg ctgtcgaata atacacgctt tctgtttctg
     6421 ctagattggt taatttatct cgaatggcaa tatcattgcc attttttaca ggattataac
     6481 gcaaatcgcc accgtccaca aagacttcaa tcgggcgatt gtatgcctct gccacttctt
     6541 ttaacgcttg taaagtctct cctgctcctt tcccatcgac taagataacg gggatacgaa
     6601 acttacacgc atgttctgct agtaattgga tcaccgtcgt ttttcctgaa cctgtagtcg
     6661 ctggaataat cacatgttgg ttcaattcac taaagttcat cacataaggg agcccatact
     6721 catctgttcc taatagaatc actttggcta atgaagccag ttgtttattc cgcaaagcaa
     6781 cgtcttttat gacaattgct acctggtacg cttgctgata ctctgctgta tatttttggg
     6841 actcctgaaa tttattcttc cgacgttcct tgtattcttt actatttagc aaagctgttt
     6901 gccgttcttt ctttgaaaca acttttcttt tttcaataaa agacgcatac gcaatcaaac
     6961 aacttcctgc acctgtccca cctaaaagga gggcaaaaat cgtttgtcca ttaaactgca
     7021 acgcatgaga aaacaagttt aatggaaata ataagtaaca cgacaaatac cctctaggaa
     7081 gaacaaaaat aaaacaaaat agatttaata gcgtaaaacc taacgcaaaa ataaaaaaaa
     7141 gagagcttct tcgttttaag aagctctcat actcaaagtc ataaatatgt gcacgtgtct
     7201 cttttttatc tatcataaag acaatcgcta aaggaatact gcctacaata aaaaatggat
     7261 atagacaaac acctaataaa gtgacgataa aataaagttg ttgaaaccga ctttttggcg
     7321 tttctttttc ttgttttctt ttgcgatcga cagaaaacat ttgctcaaaa tgtgtcggtc
     7381 gttttggtgc attcatcata ctatcgattt cctcctatcg ttttcgttgt ttcatttctc
     7441 ttcgataaat cggttgtttt tctcgttttc gtttcatttt tgcttgagtt gctcgaaact
     7501 cactccgtgt cttcggtgct tgttttggtg attttgtttg tttcatggtt gagtatgctt
     7561 tagatacggt tgaaggcgct ttggtttgtg ctgttactcg attgactgct gacatatgtt
     7621 cccgaacccc ctctggacgt cgaacagatt tcggtgtggt agaacctgtt gctcgtggta
     7681 cattcccttt tcttagtggt ttttgtaccg catgtttttg tcctgcaact gtccgggttt
     7741 tcatcgaact tgtttttgtt acgggagtaa gtgcctcctt ccgacgacga cttgtttgtc
     7801 cctgacgacg ttttcgatcg ttcaattctg ttctaggcgt atgcttctgg ccataagact
     7861 tgtgtttttg ctttggattc gctaatgaca ccaccattgc agaaccctga ttgcgatacg
     7921 gactacgaga cgcacctttt ctacttcttt ctcttagact aggattgcct tgcaattctc
     7981 gttgtaataa atcggctgtt tttcttgcct gtttctccat tgctgttcgt tgcatacgtt
     8041 cttgactttg ctcttctgct tgtttcgctt gatcatgcaa gtctcttgct ttttgtgcca
     8101 tacgttccat acgatttggt gctttatgac gaggttgttg tgcggattca cgccgaaaag
     8161 cacttcgtgt ggcttgtttc gccgtttgtg gcatacgaac gtctgagcca cccccacgtt
     8221 ttcgttgacg ttcttgtctc ttccgtgcgt tttctttttc tttttgtttc tgtgcttttc
     8281 tcgcttcttt gcgttctttc aaccgttgtt tggcttccct tctcaatttt gcttctgccc
     8341 caaatggatt tcccgcaaaa gctaacgctc ctcgaacacc cattcttcca gtaaatagac
     8401 tgcctaaaaa gcctcggaac aaataaattc ccaacggcgc aaacaaataa acaatgatca
     8461 agagaatttt ttcccatgga tcgtcttgtg tactacttaa ttggaacccg taactaagaa
     8521 aagaagaaaa aaggatacat gcaaaacctg tcattccttt taaaaacgtc aaaataccaa
     8581 aaactttggc atagttacca aacacattca ttgtgacaag aaacaatcct aaaaaaagta
     8641 agaacggcaa catcggtact aacaatagtt gcatagttcc aataatcaat cgaaacatac
     8701 ttaagacaaa ataaacaacg gtttgaatca gattcacgac aaaataaaac aaggcaaatg
     8761 ctgtattttt ccaactattg taatattgta tgttcttgtt tttcagtgta tctgcttcat
     8821 actctgtgac tttgtcatga attttctccc ctatttttgt acctaaatca ttgtccaata
     8881 aaagattgat tcgatcatat tcttttccag catatttgac tttcttctca cgaattgtgt
     8941 ctacattggt tgtgccataa tttaacaaca gatatggttc ataaacatta ctataaaaga
     9001 cccgagccgc catcaatttg cctgccgttt ccactttttt atccgctgtt acctcttctg
     9061 tttcttttgt ttcataattt gtttcttctt caggtgttaa cactggattg accttcacga
     9121 acgccgtttc cactgtagaa tctaaagaaa aaagaacatc aaaaagattc ttatttgtcc
     9181 cagaatcttt taatacgctt aacccagtaa aaataaggat cgtcataaga aagacacgga
     9241 gtgcttgttt cattcgagcc tctccagcat atttgattgc catcataatt gccgaaaaac
     9301 tgattccaat agttcctgca atacttaaca tattgtccgc aattttttgt gttaagtttt
     9361 gaatgggttc tcgaatggcg gaaataatat ctaattcaaa gagaaattca accattgaag
     9421 cattgaattc tcctaacatt tttacgccac tccaaacaaa atttttcatc actcctgcaa
     9481 cattgtacga tagctttttt ttaaggttca ttgactcata gtcattggtc atcaattcaa
     9541 acgaattctt tttgtaattt ttatacaatt gatccacatc agtcgttact tcttttactt
     9601 ctactttatc gttggcattc tctccttcag aacgatcggt ttcagtgaca cccgcaccat
     9661 cattcatttg atcggcagaa acagtagccc cttgtagaca gaaaaaaaga ccccctaata
     9721 aaaggagcgt ccaccatttc agagattttt ttcgcaaaaa aaccacctct tttttgtttt
     9781 atcgttgttt ttcattttct aacattgctt gaattttttc atcttgattt tcagtagaag
     9841 aaacagccaa taaccaacta tcaaacaaca catcaatggc aatcggttga tttcttccca
     9901 aatgatcctg gaataaacag gttcctgttt gtaattgttc catcatttct aaattggctg
     9961 aattcacttc catatcaaag aaggacaata gtttctctcg tgcttctttt tgtttcggac
    10021 gaaacgcaaa tttgtaagaa attaactctt ttttatcttc ttgatcgtaa tctttaaacg
    10081 cttgcacaac aaggtaaata tccgtattat tcgcacgtcc ttttcgcaag ctatcatcaa
    10141 tcaaatagct tccttgtgcg gtatcttcat acccttttgc ctcgtcataa ataatcgcag
    10201 cgtcttcttc tttgtttgta gaaaagatat acgtaaactt cataatgact tccataattg
    10261 ccattcctgc aagttgttcg ctatttaatc gacccgaatc aatctctttt tgtgtcggaa
    10321 tgcgtattcc ttgtgtccct aacacgttga tttgtgaatc aaaacgtatc ggcgtactgt
    10381 gctcattccc aatgagaatc tttcctaaac caacgctatg ccctaaaatc aatttagcta
    10441 gttctttgtc actttttcga atttcttcga taacttgact taacgaatgc ttacctggca
    10501 tggtgtaaac tacatcgatg gcgtcttgga tcaccgtttt cttgtggctc gctgtttctc
    10561 gattggtatc tacttctcct aacattccta acacattttt agcggtttga attgcctctt
    10621 cttttggtaa aaacaataga ggatcaagca tccctcgaca gctttcatct gaagataacg
    10681 aaataaaatt aattcgttca tacatagcca caaactctgg tatgtgtccg aatttttcaa
    10741 gtgcctttaa aaagaacttt tgggtttcat ttttcggatc aatataaaga attttttgac
    10801 ctaaaaacat cagccataaa aaaatatatt ttacaaggac cgattttcct ttacctggtg
    10861 gaccaataat caacgtattt ccattggtat gttccgcgcc ttcaatactt ttctttgtta
    10921 aattgggata ataccatact agtttactag atagcgctaa cgcttctttc gcagatttaa
    10981 acttttttgc aataacaatc cgtcctaaag gaaatccttt cttatttcct acttctttct
    11041 ttaagtccaa gccataatct gccacaaaac ctgtcgtagc gacctgctcg taactacgaa
    11101 aacgttgcgt acttccaatt aagcattgat taaacaacgt tagctgatcc gctaccgatc
    11161 gataaaattt gaaatctgta gactcaatcg caaattcaat atctttgact cgttcttcca
    11221 attcttcttt tgaagaagca gatacaacca gcgtgacggt agaataacac aatcgttttt
    11281 ctttgcgctt cagcttatct tttacatcca ttaaccgttc tgtaccaaaa tttaatacat
    11341 cgtctgaatc taaaatgccg tcaacggtct catattcttt ttcttgttca taaatccgtc
    11401 cagccatttt attaattttt cgttcatcag attttgaatg ctcaaatcga agtcgaatat
    11461 gcgtatcaat tggaaaagat aagctatcct gaagattttg aataaagcta ctcccataca
    11521 tggctaaagg agcgtccgtt tgacttaaaa acgtcgaata atgcgtttga ttcaactgtt
    11581 caatcgcaat gtatcctgct tcattcgaaa tgacgccctc gcaaatttcc attggcgtaa
    11641 gctgtctttc aggcaatttt tcttctgatc gatggaaaaa ataataataa atttgtccaa
    11701 tctcttgggc agataagcgc tctatcggct tataattgga caattccgta tagagctcct
    11761 tttctaatgt ttcatacgtt ggtaacacac tacttatagg aactcgttgt cccgtaatac
    11821 gatagataaa ctttttaaaa acatgcttaa gcaattgaaa atattcgatc ggatcagaca
    11881 ctttcatttc tgtagtgaaa cgcacaaaca gatacgttcg atactcgttt aattgcactt
    11941 cgtcttttaa aatctttcct gcctgttcaa agtacacttt cccaagatct gcgcattctc
    12001 cctttaccaa atctcgaatg gtttcttcaa tgtgcgtatc taggttaaat tgacgtggaa
    12061 tattgataaa atgatagtca tattcatcat gtccaagtat gccttcgcca tcttgaagat
    12121 agttcttgaa aaaatcaatg tcatttaaag gaaatacttg ggaagaaagt tgatagccta
    12181 cccacacatc atcatttttg gtcaaaacaa aatttcggat aatggcgtct atcggaaatt
    12241 ctaatcgagg aagcttcgtt tttttctttt tgaactgcca aatcccgata ataaagacga
    12301 ccaaaagaaa caaaaacgcc atttactcga ctcactcctt ccatttgtgt agggaatacg
    12361 tcactttttc atggaaaaac ggcacataca tttcatgttc aaagcgttgt ttccgtttct
    12421 gataatagga cgttcgccca attaaatact cgaaaaagtg tttattgtcc catttcattg
    12481 aaaacaatgt ccaaacgaga acaccaatcc caaaaatcat aatcaaccag gactttcgaa
    12541 acagtgtctg caagaaacta atttgattca catacgcaat caaaaatagg atcaaaagaa
    12601 tgaagagaaa taccccacct atgactaatt gttccatgcg aatgccatta gaaatccagg
    12661 gaattttttg tcctttaatc ccgtaaattt tgtgagggtg cttaaactcc tttgtatagt
    12721 tgtattccat gttcactcta cctcctttta caaaaaaaga agcgaccaga agttctaatc
    12781 ttctgatcgc ttttgcagtt tgtctttttc ttatgataat tgtatccagg gcacaatcat
    12841 ttgcgttacc cccacgttct cttcactatc cttaataaag ataatgttct ttagccattc
    12901 accaatatca acggcattcg ataacaataa gacgagcatt atcaaaataa agacgccaat
    12961 aatggcaaac gctgcccgcg caaagccttg ggtcgcataa gcaccaacca ttagaacaat
    13021 cgcaacgatt gccaatagcc aaatgacttg tccagataca tcatcaaata acgatttaag
    13081 atcaaaaagt ttcatttcca ttctcctcta agttcatttt attcatttaa cgtagtaaca
    13141 aaataactat cgccacttgt ttttacttcc attgaaaagt ttgattggaa ctcgtttcct
    13201 ttctcaaaat agaaacgata cttcccttgc aaaaagtagt ttccttcttt attttttact
    13261 gcatgatcca gttgtacatt gatcaattcg ccgccttcta accctttttc gttagaaatc
    13321 aatgccaatt tttcatcatt tttgacatat aactcaaaaa accgtttcac aaatgcttcc
    13381 agcttgattt cttcgctttc agtgaccgct tgcccagaag aataaaatcg attttcctga
    13441 tagttcacag aattggtatt cttactctca gaaagattca aaaattgaac gccatcaagt
    13501 acttttaaaa cattgttttg ataactgact ggaaaagaaa gttgactgct tttccaatgt
    13561 tctccttctg taaaagtaac ctcataaaat aggcgataca ttgcttgtgt caaatcgatt
    13621 tcttctagtt taataaagcg aacatctttc gctactcgat tggtagccgt cgttacaaag
    13681 cctagatcag acgcttccaa tccttgtgct aaataatgat tgacggaagc ctcccattcg
    13741 tttcgttttt ctggcgaata agtaaataac ctttcaataa aatgttgtcc ctcgtacttc
    13801 aattgttccg tagaagacaa ggtttggttg acttcagtta acaatgtttc tggattgact
    13861 tgttttttca ctgctaaacg cgttaatgta tccactcgtc caaagtttag gaggagcacg
    13921 acacccatta acagaaacaa acaaaaaaaa gcgcctgaaa atacactcat ggcacttttt
    13981 tgcgaaaatt ggatcattcg atctgacggt tctgttttct ttttttggaa caaaaaacgt
    14041 ttcttttgtt tcttttcctt ctgttgtttc ggtggcttag tttggaacgc ttgtggatta
    14101 aattttgcat tactcatgag aaatgccata ccgttctaac aataatttcg gtattttgtc
    14161 cacaatcact acttcgtgga caaccacact tgccccctc
//
LOCUS       DACDBG010000067        14152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDBG010000067 DACDBG010000000
VERSION     DACDBG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.067"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..591)
                     /locus_tag="IVZ90_002046"
                     /pseudo
     CDS             complement(37..591)
                     /locus_tag="IVZ90_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(607..1086)
                     /locus_tag="IVZ90_002047"
     CDS             complement(607..1086)
                     /locus_tag="IVZ90_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8263082.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1092..2116)
                     /locus_tag="IVZ90_002048"
                     /pseudo
     CDS             complement(1092..2116)
                     /locus_tag="IVZ90_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2133..3239)
                     /locus_tag="IVZ90_002049"
     CDS             complement(2133..3239)
                     /locus_tag="IVZ90_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8263083.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3468..6065)
                     /gene="adhE"
                     /locus_tag="IVZ90_002050"
                     /gene_synonym="adhC"
     CDS             complement(3468..6065)
                     /gene="adhE"
                     /locus_tag="IVZ90_002050"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8263084.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6370..6750)
                     /gene="yajC"
                     /locus_tag="IVZ90_002051"
     CDS             complement(6370..6750)
                     /gene="yajC"
                     /locus_tag="IVZ90_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8263085.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6847..7992)
                     /gene="tgt"
                     /locus_tag="IVZ90_002052"
     CDS             complement(6847..7992)
                     /gene="tgt"
                     /locus_tag="IVZ90_002052"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8263086.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8210..8908
                     /locus_tag="IVZ90_002053"
     CDS             8210..8908
                     /locus_tag="IVZ90_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8263087.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9076..11715)
                     /locus_tag="IVZ90_002054"
     CDS             complement(9076..11715)
                     /locus_tag="IVZ90_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8263088.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12334..13365)
                     /gene="queA"
                     /locus_tag="IVZ90_002055"
     CDS             complement(12334..13365)
                     /gene="queA"
                     /locus_tag="IVZ90_002055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8263089.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13558..>14152
                     /locus_tag="IVZ90_002056"
     CDS             13558..>14152
                     /locus_tag="IVZ90_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8263090.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDE"
BASE COUNT         3968 a         3101 c         2550 g         4533 t
ORIGIN      
        1 tagatttcta gtctaacttt tggggtgcac atcaaaagcg cctacgagat aacctcccat
       61 aaacaaagca gagatcgaag caccaccagc aacaagtgaa tacatgataa aagcagtact
      121 tggaggaatg accatacctg tcggagctga cgcgatattg accgctgtcg caaacttccg
      181 gtcatatcct tcttccactt gctgagggat caacactcca ccaatcgcgg tagaagcggc
      241 aatggccgag ctagagatgg aaccaaataa agcattccct aacacattcg tatgagccaa
      301 agcccccggc acgcgcccga cgaacaacat cgcaaaatcc actagtttct tagctatccc
      361 tccattattc atgataatgc ccgagagaac gaaaaacgga atagcgatca gcgaaaaact
      421 attgatactt cccaccattt tttgagctga actaaaagca gaaacatcaa acggtataac
      481 taacaataaa gtcatcatcg atgagaatgc aatacttata gcaatcggca tccctaatac
      541 aagcaacaca acaaatacaa aaaataacac taatccggct tctattgcca ttttattcct
      601 ccatttttat attttttctt gaattgctgg cttgttgctt aatgtatcga ttagattgat
      661 caggctatag ataatgatga gtacccccga tacaggtact gacagataaa catatcccat
      721 aggaatactt aaagaaggcg agatctgccc cattgtcaat gtgacagcat tccatccgcc
      781 atacaccatg atcatcgcag caaataccag aaataaactt tgaacaacaa cttctaaaat
      841 tttacttttt ttgccagtta atttttcact taaaaccgtt acggctaaac gtgctttttg
      901 tcccacaaca tacgcggaag acaacatggc aaaccagaca agaccgaatc gaacaagctc
      961 ttccgtgacc gtatttggat ttcctaaaac cgttctggaa aatacttgcc ataaaacaac
     1021 agcgaccatc ccaacaagca aaacagctcc caggatctct aataccttgt ttaaaatcat
     1081 tttcattaca gctacgctcc ttgctcttgg atcttttgat aaaggtcttt gtaggtagga
     1141 tcgtttttga atatatcatg aagtggggca actgcctctc gaaacggtgt attatctaca
     1201 tcattgaatt ggactccatg ttcttcttgt gctaaagctt tttcttcttc aattgctttt
     1261 gcaaaagctt caatttcaaa aaccgttgat tctttcgctg cttcttcaat ggctttttcc
     1321 tgttcatcag ttagctgttc tttgatggca tcattcatga tgatgatatc tggcacacgt
     1381 gtatgctcat cataggaata gtatttagct accccgccat gtccagctgt atacaagaca
     1441 aattcattgt tttctgatcc gttgatcaaa ttggattgaa gagacgtgta cacttcatca
     1501 cttcccatag gtacgggcga accgcctaaa agctctacca ttttgatagc ggtttcgctt
     1561 tgcatcacac ggatcttctt tcccttcaaa tcttccggcg tatggatcgg accgtctacc
     1621 atataaaagc tgcgctgacc agaatcataa taggtcaatc cggtaaatcc taaatttttg
     1681 gtcgattggt agattggctc cattatttct tcattatcca tcaccttaaa gaaatgttct
     1741 tcatcattga atagataagg aacggaaaaa atcgaataat ctttagagaa actttccaac
     1801 gctgaggcac ttacttttgt gaaatctact gctcctgact gtgtcaattc gatcagttca
     1861 cgttctccac ccagttgtcc atctgggaaa tactggatct gtacggaacc atctgttttt
     1921 tcttttacta aacgcccaaa ttcattcatt gcatcaatta ctggctgttg cttgcataag
     1981 caaagcgcaa ggtaactatc tcatttacgt ctgcatctgc gacagttgcc acactagaat
     2041 tatttccgca gccgcttaga cctagcagac ctaggctaag cgaacttgct gcaaacagca
     2101 gtgatgtttt tttcatatgg gtttcctttc ttttaacaat aagagacgag caattctgtc
     2161 aaacaaagca ccgttaacga ctgtccataa ggcattgctg tgattccgat attccgataa
     2221 aaatctaaac tgtcccccat tcctgtcccg attgaaacgt tttgaacttc tcctttttca
     2281 tcgatttgtt caagcaatcc ttggatagca cgataagcga cttctttata ttcttgtggt
     2341 aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat
     2401 tctacgtaag aagaaggatc atccaacaac gtatgccata aaccgctttc agactgataa
     2461 gtttttaatg cacgcacttg tgcttcaagt gtttcgatca ataatttgcg caatgcatct
     2521 tctttcgata attctaagat ttcaatgatc tctggaatag caatcgtcaa ccagcagttt
     2581 ccacgagccc aaaatgcttc ggcatagtta tgatttcctt caaacgtcca gccgtgatac
     2641 cacaatcctg ttcgtttatc catcaagtat ttgatatgga tcataaactg gtaacgagct
     2701 tcttctaaat attctgggcg attcaacagt ttcccgattt ttgccaaagg aagcactgtc
     2761 atcatcaatg tatcgtccca caactgattc ttattttccg gtccatatgt ggcatgctgt
     2821 aacccgtctt catttgtccg aggcatgtca ttcatgaccc attctgccca ttgttctaaa
     2881 taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt aagccatagt caatagcggt
     2941 gccattgtat tgacgttttt gggcggagcg ccttcttcca tacgatcctc aaaccattca
     3001 ttgaccactt gataagcgcg aggatttttt gtcatctggt aatttttata aataccatat
     3061 agaccgacac cttgtggcca attccacact gtccagcttt tgtcatctac tttcagtcca
     3121 tcaaagtcca acaaaaattc accattatcg tctttgatcg ttgtcaggtt ttcaacgagc
     3181 aggtcaattt tttctttcac taattcttta ggtacaaaaa tctcttcttt aataatcatc
     3241 tttacgccac ctttttaaac gctttcattt tatatcctta atatagcagt aataccgtcc
     3301 actataaata tcgctttttg aacaaaaact gtctaaaatt gacctaaatg ttaaataaca
     3361 aaagtaactt tcagttactt ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa
     3421 gttctttttc atctattttc ttatcttttt tctacgcttt cttttgctta tccgttgtaa
     3481 gcatcaatga tgatctgttt caattcagag atcaatggtt ctttcggatt agctgtcgta
     3541 cattggtctt cgtaagctaa ttcagccata cggtctacgg ttgtatctag tgtttcttgt
     3601 gtgactcctt gagcttttag actcatgttg atgccgacag attttcctaa ttcataaaca
     3661 gccgttgcta aagcttcgac taattcctct gttgtttcgc ctttcagacc taagaatttt
     3721 gcgatatctg cgtaatctgt atctgcacgg aaatagtcat atttagggaa catcgcatgt
     3781 ttttgtggat cttttgcatt atagcggata atatgcggta aaagaatcgc atttgtccgt
     3841 ccatgaggga tcccgtattc cccgccgatt ttatgtgcga ctgagtgaca aatacctagg
     3901 aatgcgttag caaacgccat accagccatt gtagaagcgt tgtgcatttt ttcacgtgat
     3961 tccatatcag gtgtttttac tgatttttct aaatagtcaa agactaactt gattgcttgt
     4021 aggctcaatc cacgtgtata atctgaagcc atgactgaaa cataggattc gattgcatgt
     4081 gttaacacat ccatcccggt atctgctgtg acagatgctg gaactgacat gacgaattga
     4141 ggatcgacga ttgcgacatc tggcgtcaat gcataatctg ctaacggata tttcacatgt
     4201 gtttcactgt cagtgatgac cgcaaatggc gttacttcag aaccagtccc tgatgttgtc
     4261 gggatacata cgaattgtgt tttttctggt ttgtcgatct tatatgtccg tttacggata
     4321 tccaagaatt tttgttttgc accaaagaaa gatgtgtctg ggtgttcata gaacatccac
     4381 attcccttag ctgcgtccat tgcagaaccg cctccaagag cgattactgt atctggcttg
     4441 aagtctgcca tcagtttcgt tcctgcgtat acggtatttg tcgatgggtt cggttcaaca
     4501 tctgagaaga cttcgatctt cacatcattt ttacgtcgtg ccaatacttc acgaactgta
     4561 tcgcagtaac cgaattggac cataccaggg tcacaaacga tcatgacacg ttcaacgttt
     4621 tccattttct ctaagtacaa tagagagttc ttttcaaaga agatttttgg cggtaatttg
     4681 aaccattgca tgttatttct ccgtttcgct acagttttga cattgattaa gtttacagca
     4741 gagacgttac gagaaacaga gttcttacca taagaaccgc atcccaaagt caatgacgga
     4801 atcatttcgt tatagatatc accgatgccg ccttctgcag atggtgtatt cactaaaata
     4861 cgacaagctt tcattcgaag accgaatttg acatgcaact cttcgttttc tgaatggata
     4921 actgctgtat gtcctaatcc tccaagattc aacatacctt cacacaaatc aaacgcatgg
     4981 tccgtgttgt tagctttgat catggcaaga actggagaaa gtttttctct tgatagcgga
     5041 taatctgggc ctacgccttc caattcagca atcagcattt tcgttccttt tggtacactg
     5101 attcctgcca attttgcgat ttccatcgca gaatgtccaa cgattgatgg attgaccgca
     5161 tattttcctt cgttcatcac tgcatcttct aatttttgca attcatctgg ttttacgata
     5221 taaacttggt gtgcttggaa ttctgctttt actgctgcat agatttcttt atccacgatc
     5281 acagcttgtt cagaagcaca aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg
     5341 ttcactgcac gtttaatttt tgctgttttt tcaatataag ctggtacgtt acctggacct
     5401 acacctaaag ctggttttcc tgtagagtaa gcagacttga ccattccagc tccacctgtc
     5461 gctaagacga tcgcgatacc tggatggttc attagcatag aagtcgcttc gatcgaagga
     5521 tgttcgatcc attggataca attttcagga gctccggcag cgatagcagc atcacgtacc
     5581 actcgtgctg cttctgcaga agatttttgg gcacttggat ggaaagcaaa aacgattgga
     5641 tttcttgttt tcaacgcgat cattgatttg aaaatcgttg tggaagtcgg attcgttgtt
     5701 ggagttactc cgcaaaccac accgactggt tcagcaattt caatcaaccc tttttggaca
     5761 tcttcattga tcacaccaac tgttttatca tgtttgatat tatgccaaat atattcggac
     5821 gcatacatat ttttgattgc tttatcttcg taaacaccgc ggcctgtttc ctctacagcc
     5881 atcttcgcta atggcatatg ttgatccaaa gctgccattg ccatttcatg cacgatatgg
     5941 tctacttttt cttgatcaaa gctttccatt tctttcaatg ctgcatttgc tttttctgcc
     6001 aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt tttctttttc tattacttta
     6061 gccatcatta tcctcctaat gggtcatggt tatttgttaa ataattcaca tgtatatcat
     6121 acaatattta tttttctatg taaacacttt tttgtgattt ttttcacagt tatttttaaa
     6181 tataaaattg ctttatttac aggtgtttgt atagaaaaat atataaagtt atttattatt
     6241 atttatatga cacacccgat tattcaatat tgagcaatat atcccatgct cttatctctt
     6301 tcttttttga ataaaaaatg aggtaggaca tctttctgcc ctacctcgtc tttaaataag
     6361 ctttgatact tatttttctt ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt
     6421 ttttacttca actgtctcag ctggttcttc tgttgtttca acagtagtcg ttgcatcatt
     6481 tgttacgaca gtccctggtt tgaccgtttt gattgctgca cgatcgtact caaggaagat
     6541 cccttcgcag tctaaaacaa ctgtccgttt gtcattgtcg atttctgaaa caactccatg
     6601 taatccgccg atcgttacga ctgaatcacc tggtttcatg ctatctaaca agttttgacg
     6661 ttcttgctgt tgtttctttt gcgaacgtga cataaaaaac cacattgcta atagcgcgat
     6721 aaacatcaca agcattggta atgttcccat ttttttcacc tcactaattc tgtatctaca
     6781 aaaaacactt tagcagaaaa atgctgataa cactagtttt ttacttaatt tttaaaagat
     6841 tttcctttag aagctttttg cgttttcttt attgaacccg tattcttcaa aaaaggcttc
     6901 tctaaattca agcaaattat catccatgat tgcttgacgt acttgtttca ttagattcaa
     6961 caagaagtat aaattgtggt aagaagtcaa acgaatccca aatgtttcat cacatttgat
     7021 caaatggcgg atataggcac gtgtatagtt tttacatgta taacaatcac atttctcatc
     7081 aattggacgg aaatcatggg catatttcgc atttttcact actagacggc cttgagaagt
     7141 catacatgtg ccatttcgag cgattcgagt aggcaagaca cagtcaaaca tgtctactcc
     7201 acggataaca ccatcgatca atgaatcagc agcaccgaca cccatcaagt aacgtggttt
     7261 gttttcgggg atcaacggtg tagtgaattc taatacacga ttcatttctg atttaggttc
     7321 cccgacagat aacccgccga ttgagtaacc agggaaatcc atcccgacta gatctttcgc
     7381 gctttgacga cgaagatctt caaaccctgc tccttggatg attccaaata atccttgacg
     7441 gtctggatta gcatgagctt tcaatcctcg ttctgcccat cgagacgtac gttctactga
     7501 ttttttcacg tagtcgtaac tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat
     7561 atcagagccc aatttatttt ggatgttgat tgctttttct ggtgataaaa acatacgcga
     7621 accgttcaag tgatttttga aatgcacgcc ttcttcttcg atattccgca tatcacttaa
     7681 tgaaaaaact tgaaagccgc ctgaatctgt taaaatcggt tgatcccagt tcataaattt
     7741 atgcagtccg ccagcttctt ctactaagtc ttcaccaggg cgtagccata aatgatatgt
     7801 attacttaaa ataacgccag cacccatttc tttcaactct tctggggaca ttgtttttac
     7861 tgtagctaat gttcctactg gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat
     7921 caattcccct aaacgcgcgc cagtatgttt ttctttcttg attaaacgat agcgaatggc
     7981 aggttgcgtc attcatttcc ctactttcta tataaaatat cttgacaact cccaccatca
     8041 taatcgtttc aagtgctttt tgcaagttcc ctcggcttcc ttctacttta tttaatattc
     8101 ttttttttat ccttttttta tgaataacaa aaaaaagaaa aacattatga cagaaatatt
     8161 ttttgataca tggtatattg gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc
     8221 tgcagaatta aaacgccaag caaaagagag tctaaaagga cgttggggac aagctgtttt
     8281 actaaatcta atccctacat tgattggtat tgctactatt cttcttatta tgattcctat
     8341 tatcttgttt gccaccacca tatatgatgg atcagcgagc gtctcagaag cgacaacttc
     8401 aggaggcggt agttcaggag gcggtggcgg aattatttcc agcatcatta gcgctttgtt
     8461 catgagcggt atttcttgga cttatcttga tattttacgt ggacaaaaag atcagatcga
     8521 accattcaaa gatgcatttc gtggattcaa aggtttgttt atcggcggag tgatcagctt
     8581 agctgtgctg atctcgatct ttacaacatt atggggtttg ctattagtta ttcctggaat
     8641 cattaaaagc tattcttatt ctcaaagcta ttttatttac tacgatatcg tccaagaaac
     8701 aggacaaaca cctaaagtac tggatacgat cactgcaagt cgaaaattga tggatggtta
     8761 taaaggacaa cttttctggt tagatctttc atttatcggc tggcatatct tagcattagc
     8821 aactgctggt attggttatc tatggctcag cccatacatt tctgcaacaa aagcagcatt
     8881 ttatgaacaa ttgccaaaag atatttaaaa gagagtgtga cttgtgaccg agggggtggc
     8941 aggagaacac cgtttatcga gaggcagtaa aagaaacatt cagcccatta gcaaaaagga
     9001 ggtgtgacaa cattcatgtc acacctcctt tttgtatagt tccagtaaaa agaaaatcaa
     9061 cttagaaaca gttaatcatt cctcttcttc tgcttccggt aatcgatgga acttgatcag
     9121 ttcattcacc gcgattggta cgatagataa aatccctacc aagaaccagt gattcaaact
     9181 gatgtgtact aggccgaata gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat
     9241 agctaatgcc agtaacgcca tttctaccaa agctttattg gtcgctaaat tgactttgaa
     9301 gatcgattgg ctgctgcgta cattgaacac atgcagaatc gatgaataag ctaagatcaa
     9361 gaatgcaaca gtttgaccga catgctgact tgcgtctacc catgggctga tagtatcgac
     9421 ataactacct aaataataac caaacaaggt tacgatggtg aagacccctg catttatggc
     9481 aattttttgc aataaacctc gagcaaagat cccttcattt ttcggaatgg gtggctgatc
     9541 catgatgccg tgttccgctg gctctcttcc taaagcaaat cctggaagtc catcagcaac
     9601 tacattgaca aacagtagtt gcacagctgt aaatggtgct ccccatccta acagcatcgc
     9661 aatcaaaaca ataaagatct ccgaaatatt acagcttagc aaaaagttga tagctttacg
     9721 aatattctga taaaccgaac gtccttgact gacagcgtct acgatcgtcg caaagttgtc
     9781 atccgtcaaa atcatatctg cggcactttg cgccacatct gttcctgtga tccccatggc
     9841 acacccaacg ttacttgctt tcaacgctgg tgcatcattc acaccatccc cagtcatcgc
     9901 tacgactgca cccgtacgtt gccaagattt gacgatccgg atcttgtctt ctggcgttac
     9961 ccgagcatag acagagagag ctttgacacg cgcatctaat tcttcgtctg ataattgatg
    10021 cagttctgat cctgaaagtg cttctgacgg atcattcaag atacctaatt ctttcgctat
    10081 ggcactggca gtcaccacat gatctccagt gatcatgact gttttgatgc cagccttttt
    10141 cgctcgcttg attgctcctt tactttctgg acgtggtgga tcgatcattc cgatcagacc
    10201 aatcaaacgt aagtcttttt ccagtgcttc agaagaaata tcttgcggtt cttcgtcata
    10261 agtagcataa ccaacagcaa tcactctcag tgcccgttta ccaaagcgat cattaacgat
    10321 cgccgcttgt tctacatcgc ctgaactaaa gcgcggagca agaacatcga atgctccttt
    10381 ggtgattgag atgtattttt cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga
    10441 atcaaaagga agctcgttta ctcgtggata tttttcttct aattctgcct ttgtatgata
    10501 attctcttca acagccctta cgatggctac ttctgttgga ttacctttga taacaaggtc
    10561 gtcatcttct tgctcgaccg tcacatctgt acatagtgca gccattttca atatttccat
    10621 cgcgctgttc gtcattgaat cttcgatatt cgttacttca tcttctttgc tccatacccg
    10681 gcggacgcgc atttcatttt gtgtcagcgt tcctgttttg tctgagcaga taacattagc
    10741 ggttcctagc gtttcaactg ctggcaactg tcggatgatc gcatgtttcc tcgccatctt
    10801 ctgcacaccg tatgcaagtg tcaacgtaac aataacagtc aaggtttccg gtacagctgc
    10861 aacagccaag gaaatcgaag tcatgaacat ctcaaggagt ggttctcctt gcagttcacc
    10921 aatcacaaat actaaagccg cagccgctaa agcgatcata ctgattcgtt tgcctagctg
    10981 attcaatcgg acctgtaaag gtgttttttg catagtattt tcgttcaaaa gatccgcgat
    11041 cttacccatt tctgtagcca tgccaatggc agtgacgatc gctttcccac gacctgcagc
    11101 aaccgtacat cctttaaata ccatattaag ttggtcacct aatgactcat cttcttcagc
    11161 aataaatgct gcatcttttt caactgcttc actttctcca gtcaacgccg attcttctac
    11221 acgtaattgt gatgcttcga tcaaacgggc atcggcagaa atagcatcac cggactctaa
    11281 aacaagaata tcacccacta ccagttgctc tgactcaaca gtggtcactt tgccgtctcg
    11341 tatgacagtt gccgtttgtt tattcatatc ttgcaaggat tccagtgctt tttctgcgtt
    11401 tccttcttgg acaatcgcca ataccgagtt gatcacaacg atagcgatga tcagcagtcc
    11461 ttcaaataaa tccccatgtt ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac
    11521 aagaaggatg atcgttgtaa aatcggacaa gcttcgaaag aattttttga tcatcgattc
    11581 tttcggcgcc tcatcgaatt tattcaatcc ctgctttttt cgccgttcat ccacttcttg
    11641 tgaagacaac ccagtatctg gatctgcttc tgttaccttc tttacttctt ctattgtctg
    11701 cttgtattct tccatcctat tcctccttta aaatcttcgt acaaaaaaag gtacttattt
    11761 tactataaca tccttttttt atttacttca aataaagggc ttatcgatta tttgttaaga
    11821 aagcgtaaaa tttaaagtgc ttatatttgc aaacgctacc aaaggatgct attatataga
    11881 tgtaaggaag gaggatacca tcaaatagac agaagaattt ctcgatagaa cagtgtaagc
    11941 aaacaaagtt gttagaagta caggtttcac ccagacatgc gtgtagagta caaacctata
    12001 aatagtgaat gcgtatggat gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat
    12061 acgtaacaat tgaataacgt aaacgtctga aaattgaata ggaacaagag atgtttcaaa
    12121 tgttgaagat gtaacgaaaa caaaattttt gtttaacatg aagaaattca acaccgtgct
    12181 ttgcatgtat gatggaaagt tacaatcatt tctgtttatt tgaatccttc cgaggtacaa
    12241 gaaagccgag cttatcgaga gctcggcttt ttctttgtca taaaatcttt gtaaagcttt
    12301 tttgttattc aaaactagcg gcttttcata accttatttc acgaacatcg catctccaaa
    12361 actaaagaaa cgatagcgct cttcaatcgc atgctggtaa gcagataaag tcaattctct
    12421 tcctgcaaag gcactaacta acatgactaa agtcgatttt ggcaaatgga aattagttga
    12481 gaaagcttct accactttga attcatagcc aggcgtaatg aaaatatcag tccatccgct
    12541 gtctgctttg atttctccat taaatttcgt tccaatcgtt tctaatgttc gaattgaagt
    12601 tgttccgaca gccacgattt tcccgccatt tttacgtact tggttcaatc gctcagccgc
    12661 ttcttccgtc aaacggtaga attcactatg catatggtgt tcttcaattt tatctacgct
    12721 gacaggtcgg aatgtaccta accctacatg tagtgtcaga tagactaatt caacaccctt
    12781 tgcttcgatc tcatcaagta attctttcgt aaaatgcaat ccagctgttg gcgctgcagc
    12841 agaaccattt tctttcgcat agacagtttg atagcgatct gggtcttcca gacgttcttt
    12901 gatatatgga ggcaatggca tttctcccaa tgattcaaga atctctagaa aaattccttc
    12961 atactcaaag tcaatgatcc gtccgccgtg ttccaattct tctttcacta ctgcttgcaa
    13021 tctgccatca ccaaaagaga ttcttgtccc aactttggca cgtttcgcag gtttgatcaa
    13081 tgtctcccac gtatctcctt gtgtattcgt tagaagaagg acttctaaat gcgcaccagt
    13141 atctggtttt tctccatata aacgagcagg cagaactcgt gtattattca taactagcgc
    13201 atcaccaggc tgtaactctt caatgatttc atggaaatgt ttatcttcca tctctccagt
    13261 tttccgatcc aaaacaagta aacgagaatg atcgcggtct tttaatggtg tttgtgcgat
    13321 taattcttcc ggcaagtcaa aatcaaaatc ttctgttgtt aacatatcgt cactcacttt
    13381 ctatcatgtg cttcatcatt ctatcatgtt tttcgttctg ttggcaaaat cagcactttt
    13441 tcacttctat tttttgtttt cctaaataaa aaaactaccc tatttgtatt ttttcattgt
    13501 tttaggcata ctagtaatag atagttcttc attgaaaata gaaaaagagg tggcaccatg
    13561 attgaatttc aaaatgtgtc aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc
    13621 aatctttcat ttgaaaaggg tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa
    13681 acgacgacga tgcggatgat caaccgaatg actgatccaa cacaagggaa aattttgatt
    13741 gatggaaaat ccatccaaga aattgatcca gtggaattgc gacgtcagat cggctatgtt
    13801 atccaaagta ttggattgat gcctcacatg acgatccgtg aaaatatcac tcttgtccaa
    13861 aaattgttga aagtcagtca agaagaacgg aataaaacag ctgaaaaaat gattgatctt
    13921 gtggaattgc cccgtgagat gttagatcgc tacccacatg aattatctgg tggtcagcag
    13981 cagcgtattg gagtcgttcg tgcccttgca gcgaatcaag atatcatctt gatggacgag
    14041 ccattcggtg cgcttgatcc aatcactcgt gattccttgc aagaccttgt caaagattta
    14101 caagagcgtt tgggaaaaac gatcgtattt gtaacccatg acatggatga ag
//
LOCUS       DACDBG010000068        13620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDBG010000068 DACDBG010000000
VERSION     DACDBG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.068"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IVZ90_002057"
     CDS             <1..848
                     /locus_tag="IVZ90_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8263091.1"
                     /translation="IESSEHKYDFKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPE
                     VHEGGHLDTLTTSVVFLVFKNKLIIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIM
                     QKDFYMMLNDLRGVKEKIDDLDSEISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKA
                     LDFIKKNFTTIDDIDFSTKKIIDDLYDTSDTMDRIILSYSQYLDKLENMINNMISYQL
                     NMIMKTLTEISIVLTIPAIIFGFWGINVHVPFEKSSYGVLAVLIISAILSLICWLLMR
                     RKTYL"
     gene            1366..1821
                     /locus_tag="IVZ90_002058"
     CDS             1366..1821
                     /locus_tag="IVZ90_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8263092.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2132..2488
                     /locus_tag="IVZ90_002059"
                     /pseudo
     CDS             2132..2488
                     /locus_tag="IVZ90_002059"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2501..2911
                     /locus_tag="IVZ90_002060"
                     /pseudo
     CDS             2501..2911
                     /locus_tag="IVZ90_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3026..3190
                     /locus_tag="IVZ90_002061"
     CDS             3026..3190
                     /locus_tag="IVZ90_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263093.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(3341..4320)
                     /locus_tag="IVZ90_002062"
                     /pseudo
     CDS             complement(3341..4320)
                     /locus_tag="IVZ90_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4325..5092)
                     /locus_tag="IVZ90_002063"
     CDS             complement(4325..5092)
                     /locus_tag="IVZ90_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8263094.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5582..6007
                     /gene="lacA"
                     /locus_tag="IVZ90_002064"
     CDS             5582..6007
                     /gene="lacA"
                     /locus_tag="IVZ90_002064"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8263095.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6024..6539
                     /gene="lacB"
                     /locus_tag="IVZ90_002065"
     CDS             6024..6539
                     /gene="lacB"
                     /locus_tag="IVZ90_002065"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8263096.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6550..7482
                     /gene="lacC"
                     /locus_tag="IVZ90_002066"
     CDS             6550..7482
                     /gene="lacC"
                     /locus_tag="IVZ90_002066"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8263097.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7485..8465
                     /gene="lacD"
                     /locus_tag="IVZ90_002067"
     CDS             7485..8465
                     /gene="lacD"
                     /locus_tag="IVZ90_002067"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8263098.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8493..8810
                     /locus_tag="IVZ90_002068"
     CDS             8493..8810
                     /locus_tag="IVZ90_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8263099.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            8810..10516
                     /locus_tag="IVZ90_002069"
     CDS             8810..10516
                     /locus_tag="IVZ90_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8263100.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10658..12064
                     /gene="lacG"
                     /locus_tag="IVZ90_002070"
     CDS             10658..12064
                     /gene="lacG"
                     /locus_tag="IVZ90_002070"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8263101.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12087..12359
                     /locus_tag="IVZ90_002071"
     CDS             12087..12359
                     /locus_tag="IVZ90_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP8263102.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12380..13279
                     /locus_tag="IVZ90_002072"
     CDS             12380..13279
                     /locus_tag="IVZ90_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8263103.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4403 a         2290 c         2652 g         4275 t
ORIGIN      
        1 atatcgaatc ttctgaacat aaatatgact ttaaaatagg caatatacta acgtttgatg
       61 atcgagttgc atatgacacg atgatagata agaacgatga aaaaagtttg ctcgtaagtt
      121 ttttgtttcc cgaggtacat gaaggtgggc atctagatac cctgacgaca tctgttgttt
      181 tcttggtatt taaaaataag ttgataattt ttacaaaaca aaaattaaaa ttcattcccg
      241 aactcttatc caatatggtt ttacgtgatg tcaatcattt cgtggaggaa gtacttttaa
      301 agatcatgca aaaagatttc tatatgatgc ttaacgacct tcgtggagtc aaggaaaaaa
      361 ttgatgattt agactcagaa atatcaacgt ccggttcact caaaccaacg tttggtgatt
      421 tattgactct tcaaaaatac atgatagctt tgtcaacaac ttataaagct aaccataaag
      481 cgcttgattt cattaaaaaa aatttcacaa ctattgacga tattgatttt agtactaaaa
      541 aaataattga tgatttatac gatacaagtg atacaatgga tagaatcatt ttaagttaca
      601 gccaatattt ggacaaattg gaaaatatga taaacaatat gatttcgtat cagctaaaca
      661 tgattatgaa aaccctaacc gagatatcaa ttgttttgac tattccagca attatttttg
      721 gcttttgggg aatcaatgtc catgtcccgt ttgaaaagtc ttcttatggt gttttagccg
      781 tattgattat ctctgctata ctaagtctaa tttgctggct tttgatgcga cgcaaaacat
      841 atttgtaaag tatttgtgat accctaatat gatttaaagc agaatttctt tgttgacttt
      901 ttaaaaggct gattcctttc aataatttaa tagcttcagc ggctgctcag aagtcaataa
      961 attggactat ttttattcag ttacttaggc aacatttgag atacgattgc gattataaaa
     1021 attgatatac caaccaatgg ctgcttggaa gactgacata ttagcctcga tatcatcgaa
     1081 taactaaata cagaaaaata caataaatat tatcaaacta gggactatac tagaaaacat
     1141 cagtacattc cttaactcag ggtctataat tagaaaatcg cccctttgaa aactgattaa
     1201 aacagccccc attatctagt gactagataa tgggggctgt ttttttgtta agtgatataa
     1261 taaaccataa aagaagttta gaaatacggt cctggtcatc aataaataaa caatttaatt
     1321 aattatttat ttaattattt aatttcagaa aggtatacat gaatcatggt gcaacaaatt
     1381 gtcctaccca tcaaagactc cagcgtctta aagatggtac aagatacact tctcgatagt
     1441 ttccgtgctg gtcgccgaaa ttacaccgtt tttcaagttg gtaaggccac gctactacgt
     1501 gtgagtgatg tcatgacatt aaaaaaatca gatgtctata atccagacgg ctctgtcaaa
     1561 aatgcaacct ttattcatga taaaaagacc ggtaaagcaa acacgttata tttaaagcct
     1621 gttcagcaag acttgttgca atatcatgat tggctggccc aagaaaatat caattctgag
     1681 tggttatttc cctcgacagc ccattatgat cttcatatca ctgagaagca gtttcataag
     1741 gtgatggcgc atgttggtga tctcttagga atcaacttgg aagacaaaga gaaaaaattg
     1801 cgagtcagaa aaccgttttg agatatgaac agcatttgcc ttcacgcctt agcgatttta
     1861 aataatagcc ttgaaagtga ttatggaaaa gccctctacg ctcaatgcaa aattgacgta
     1921 gaaactattt ttgatcgatt gaaaggtgta tttggtatgt gaagaaccca tgtcagaggc
     1981 aagaacgttg cacctactga cattggaatg atgcttatga gcatgaatct aaccaaatta
     2041 tcccttgagg ctagaagaaa agcggaagct tttcaacaca aagcagccaa aaataaaaaa
     2101 cgcgacgaag caatcacatt tatgattatt catcgcgttt tttatattta gagctagtta
     2161 tttgctagac ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt
     2221 ggaattagca gaaccaggtc attatgacga aaagtggcaa aattggaaac ttgaatcttt
     2281 gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa
     2341 aattgttgca gaacttctca aaaaggcaaa tacaattatt gttgcaacag atagcgacag
     2401 agaaggtgaa aatatcgcct ggtcgattat ccataatgca aatgcctttt caaaagataa
     2461 aacatttaaa agactatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca
     2521 aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat
     2581 tgccgattgg ttgatcggca tgaacgcaag ccctttgtat acgttaaatt tacaacagaa
     2641 gggcgtacaa ggtacatttt cactaggacg tgttcaaacg cccaccttat accttatttt
     2701 tcagcgccag gaagccatag agaattttaa aaaagaacct tttttcgagg tggaagctaa
     2761 gtaaaacaag ggtcatttaa gggcgttcta agccccacac agcgttttaa aacccaagag
     2821 gagcttttta gcttttgttt cttctaaaca agctaaaata ggcaatcaag aggggataat
     2881 tgctgatgtt caaaccaaag agaagaaaac gaatagtccg agttcgtttt ctttaagtag
     2941 tttgcaatca aaagtcaatc agctttataa agcgacagcg agccaaactt taaaagctat
     3001 gcaaggactg tatgaagcaa aattattgag ttatccaaga acagatacac catttattac
     3061 agagaacgaa tttgcttatt taaaagcgaa ttttggcaaa tatagcggtt ttttaggact
     3121 tgatcttgaa atgttgttat cattgataat gatacgttca aacaacagca cccaaatttt
     3181 gacgaattag tgaaactcta tgaaaaagac gtagtaaaac atgcaacatc ctattctaat
     3241 cagctggtaa agcttaatta ggttatataa gcttgaaaat aaagggtttg tccaatatgg
     3301 acaagccttt ttcttatgtt ttgccacaga tttgccacac ttattgtgta agagtatcaa
     3361 ggataggtat aatttgatac tttgtttttt tagtgatgtg caaataaatt tctgttacac
     3421 gactacctct agaatgccct acacggtctt gaattgcttc taggggtatt ccttactctg
     3481 ctaagaaact aatatgcgta tgacggaata tatgggtcgt aatatgttta ttaaaactaa
     3541 gtttattatc aatatcaatt cctgctcgtt ttggattacc ttttcgattt aattttccat
     3601 tttcaggtga gttgaggaaa ttgttaagga cactataatg aagaggtgtt ccattatagt
     3661 cgggtttatg acgacttttg gaagtaaaaa tccatccatt atctttataa tcaggattga
     3721 atctcatatg ttggtgattt aactcaattt cctctttaag aatttctata gcccttttag
     3781 ataattggac atttcggtaa ctttgtaagt tttttacatt accttcatag ctattttctg
     3841 cagctcctcc gtgagtggac tgactaatga tgatattttg cttttcaaaa tattgatagg
     3901 caccaatatt ttggagtttc gactctgtaa gtcaacaggg ccattagtta gaatacctat
     3961 tgggatgcgg tttttatgta gagccagcag tagttgatgt atctcaggaa tcatcgtaat
     4021 ctgatcgagt tcatattcat agtcagcttg gaagtctaga gcttctgtcc tagaaatact
     4081 atcgatgcca aattcttcca tagtcttctg acagcgaaaa attctaagct gctcaatact
     4141 gatgagtttt ttcgtgtatt taggaaaagc aacatcagac caatatctga aacgtttgta
     4201 aatgttatct atttgttgga tatcaatcgt tggaaaacat ttctcaacgc tacgtttaaa
     4261 ggggagttga tggtcataaa gagtgtcatc aaggtcaaaa acaatatttt tataagtcat
     4321 aattctagtc cgcttttaaa atagtagtgt actggctaaa ttcctctaag tgttgagggc
     4381 taatctggtt atctgtaatg atggtatcga gttgatctag attatagaag ttaaagaaat
     4441 cgtatcgatc gaatttagta ctgtctacta ataagaattt ttctacagca ttgtttaggg
     4501 caagttgttg aatcacacct tccttgtcac tatatgtagc aatagaatta tgggtaacag
     4561 catttgcact aacaaaagct ttggcaaatc taattgcttt taaatttgtc gaagccattg
     4621 aacctacaaa agctccagtt atttctctat attcaccgcc aagaagcaat aaatcaatgg
     4681 tttcgctatc atttagaatc aagaacacag ggagactatt tgtaatgaca cgaattttat
     4741 aacctttacg acctttcaat tctaatgcca gttgttcaag tgtagttcca ggtccaataa
     4801 aaatagtatc gccatctttg attaaagagc aggctttttt agcaatgtca attttttctt
     4861 ttgtatttag acttttcttc tcgatatgtg tcttttcaag tggcttttta gaggaaagca
     4921 attgtgcacc accatgagta cgtgttaaaa gtccatcagc ttctagagca tcaaggtctc
     4981 tacgggctgt catatcggaa atatctagtt catctattat ttcttttatg gttatggtcc
     5041 catcaatctt taacatatct aaaatttttt ctaatcgtcg ttttttgttc atatgaagac
     5101 ccttctttca taaagtaatt tttttccaaa gataattctc ttttaattgt atcataaaag
     5161 acaatatttt caaggtaaaa caaacaattt caaacaaaaa caaatgttag atgatgaaat
     5221 aagaacagag gattgacgta tattagctta ggtcagattt tgtataagac gaaaataaag
     5281 taggacctct taatcagtaa gttatagaaa gtaaaagact tttgtaatac ttgaatagat
     5341 atttcacgtc cattttgtga tggattaaat gaacaaaaat gaacaataat ttaacggtgt
     5401 tatctatttt ttaaaaaaac aaataaaaaa aacaaaaaat aaacaaaaaa agtgttgctt
     5461 tttgtttgaa tgcttgatat tatataaaca aagaaatgat gaaaacgttt tttcgaacat
     5521 tttgcaaaat gttttctact tctacgtagc atttctttct aaaatttagg aggtggtcaa
     5581 aatggctatt gttgttggtg cagatctcaa aggtacgaga ttaaaagatg ttgtgaaaaa
     5641 ttttcttgta gaagaaggtt ttgaggtaat tgatgtgact aaggacggac aagattttgt
     5701 tgatgttacc ttggcagttg cctctgaagt aaataaagat gagcaaaact taggtattgt
     5761 aattgatgct tatggagctg gtccattcat ggtagcaact aaaattaaag gtatggttgc
     5821 agcagaagtt tcagatgaac gctcagctta tatgacacgt ggacataaca attcacgaat
     5881 gattacggtt ggcgctgaaa tcgtcggtga tgagcttgct aaaaacatcg ctaaaggttt
     5941 cgtcaatggt aaatatgatg gcggacgtca tcaaatccga gtagatatgc ttaacaaaat
     6001 gtgttaagaa aaggagttaa ggaatgagaa tcgcaattgg atgtgaccac attgtcacag
     6061 atgaaaaaat ggcagtatca gaattcttga aatcaaaagg acatgaggtc cttgatttcg
     6121 ggacatatga ccacgcacgt actcactacc ctatctatgg taaaaaagtc ggtgaagcag
     6181 tagtaagtgg tcaagcagac ttgggagtat gtatctgtgg tacaggtgtc ggcattaaca
     6241 atgcagtcaa taaagttcca ggtgttcgct cagcgttggt tcgtgatatg acatcagcta
     6301 tatatgcaaa agaagagtta aatgcgaatg ttatcggttt cggtggaaag attactggtg
     6361 gtcttcttat gaatgacatc attgaagctt tcattgaagc tgagtacaaa ccaacagaag
     6421 aaaataaaaa attgattgca aaaatcgaac acgttgaaac acataatgca catcaagcag
     6481 atgaagattt ctttacagaa ttccttgaaa gatgggaccg tggtgactac cacgattaag
     6541 gtataacaaa tgattctgac agtcacacta aatccttcag tagacatctc ttatcctcta
     6601 gaaacattga aaattgatac agtgaatagg gtgaaagatg ttagtaagac agctggtgga
     6661 aaaggcttaa atgttactag agtcttgtat gaatctggtg ataaagttac tgctacaggt
     6721 ttcttaggtg gtaaaattgg tgaatttatt gaaagcgaat tagaacaatc tcctgttagt
     6781 ccagcttttt ataaaatctc aggaaacact agaaactgta ttgctatctt acacgagggt
     6841 aaccaaacag aaattttgga acaaggacca acaatttctc atgaagaagc agaaggcttt
     6901 cttgaccact atagcaatct tattaaacaa tcagaagttg tgacaatttc aggaagttta
     6961 ccttcaggac ttcccaacga ttattatgaa aaacttatcc aacttgcttc ggatgaagga
     7021 gtagcagtcg ttttggattg ttcaggtgca cctcttgaaa cagttctaaa atcgtcggct
     7081 aaacctactg ccatcaaacc taataatgag gaactttcac agttgcttgg aaaagaagta
     7141 acaaaagata ttgaagaact caaagacgtt cttaaagagt cactttttag tggtatcgaa
     7201 tggatagtgg tctcattagg tcgaaacggt gcctttgcaa aacatggtga tgttttctat
     7261 aaggtagata ttcctgatat cccagttgtt aatccagtcg gatcagggga ctcaacggta
     7321 gctggtattg catcagcttt aaatagtaaa aaaagtgacg ctgacttatt aaaacatgcg
     7381 atgacattgg gtatgttaaa tgctcaagaa acaatgacag ggcatgtgaa tatgactaat
     7441 tacgaaacac taaactcaca aattggagta aaagaggtat aaaaatggta cttacagaac
     7501 agaaacgcaa atctttggaa aaaatttcag ataaaaacgg tgttatctca gctttggcat
     7561 ttgaccaacg tggtgctttg aaacgtttga tggcacagta tcaagataca gaaccaactg
     7621 tagctcaaat ggaagaacta aaggttttgg ttgctgatga acttacaaaa tacgcttctt
     7681 caatgttgct tgaccctgaa tatggacttc cagcaacaaa agcattggat aaagaagcag
     7741 gtcttcttct tgcctatgaa aaaacaggtt atgatacatc aagtacaaaa cgtttgcctg
     7801 actgcttgga tgtttggtct gcaaaacgta tcaaagagca aggcgctgat gccgttaagt
     7861 tcttgcttta ctatgatgta gacagctcag atgaacttaa ccaacaaaaa caagcttata
     7921 ttgaacgtgt tggttctgaa tgtgtcgctg aagatattcc tttcttcttg gaaatccttg
     7981 cttatgatga agaaatctca gatgcaggtt ctgttgaata tgcaaaagtg aaacctcgca
     8041 aagtcattga agcgatgaaa gttttctctg atccacgctt taacattgat gttcttaaag
     8101 tagaagtacc agttaacgtt aaatatgtcg aaggttttgc tgatggtgaa gtagtttaca
     8161 gtaaagctga agctgctgac ttctttaaag cacaagaaga agcgactaac cttccataca
     8221 tctacctaag tgcaggtgta tctgctaaat tgttccaaga aacattgcaa tttgctcacg
     8281 actcaggtgc caagtttaat ggtgtgcttt gtggacgtgc tacttgggca ggatctgtag
     8341 aaccttacat caaagaaggt gaaaaagcag cacgtgaatg gttgcgtact actggattcg
     8401 aaaatattga tgaacttaac aaagttcttg ttaaaacagc tagtccatgg actgataaag
     8461 tatagtaaaa acataaaacg gaggatattg ttatgaacag agaagagatg actctcttag
     8521 ggtttgaaat tgttgcttat gctggagatg ctcgctctaa acttttagaa gcgcttaaag
     8581 cggctgaaaa tggtgatttc gctaaggcag atagtcttgt agtagaagca ggaagctgta
     8641 ttgcagaggc tcacaattct cagacagcta tgttggctcg agaagcttct ggggaggaac
     8701 ttccatacag cgttaccatg atgcatggtc aggaccactt aatgactact gtcttattaa
     8761 aagatgtgat tcatcacctc atcgaacttt ataaaagagg agcaaaataa tgcataaact
     8821 cattgaactt attgagaaag ggaaaccgtt ctttgagaaa atctctcgaa acatctatct
     8881 tcgtgctatt cgtgatggat ttatcgctgg tatgccagtc atccttttct catctatctt
     8941 tatccttatt gcctatgtcc caaatgcttg gggattccac tggtcaaaag acattgagac
     9001 cttcttgatg actccttata gctattcgat gggtatccta gcattctttg ttggtggtac
     9061 taccgctaaa gctttgacgg actcaatgaa ccgtgaccta cctgcgacca atcagattaa
     9121 cttcttatct acgatgctag cgtctatggt agggttcctt ttgatggcag ctgagcctgc
     9181 aaaagaagga ggcttcttga cagccttcac gggaacaaaa ggtcttttga cagcatttat
     9241 tgcagccttt gtgacagtta atgtttacaa ggtctgtgtg aaaaataatg ttactattcg
     9301 tatgcctgaa gaggttccac caaatatctc tcaagtattt aaagacttga ttccattcac
     9361 tgtagcggtt gttctccttt atggatttga actcattgtt aagggaactc ttggtgtaac
     9421 cgttgcagaa tcaatcggta cccttcttgc tcctctcttc tcagctgcag atggttacct
     9481 tggaattaca cttatctttg gtgcttatgc cttcttctgg tttgttggta tccacggtcc
     9541 ttcaattgtc gaaccagcaa tcgctgctat cacttatgcc aatatcgatg ccaacttgca
     9601 tcttatccaa gctggacaac atgcagacaa agttatcact tctggtactc aaatgtttat
     9661 tgttaccatg ggtggaacag gagctacatt gattgttcca ttcttgttca tgtggatttg
     9721 taaatcagaa cgtaaccgtg ccatcggacg tgcctcagtc gttccaacat tctttggggt
     9781 taatgagcca atcttatttg gtgcaccaat cgtattgaat ccgattttct ttgtaccgtt
     9841 cattttcgct ccgattgtca acgtttggat ctttaaattc tttgttgaca cattgaacat
     9901 gaactcattc tctgccaacc ttccatgggt tactcctggt ccgttgggaa ttgtactcgg
     9961 tactaacttc caagttctat catttatctt agccggactc ttggtagttg ttgatactat
    10021 catttattac ccatttgtta aggtatacga tgaacaaatt cttgaagaag aacgttctgg
    10081 taaaacaaac gatgctctta aagaaaaagt agctgcaaac ttcaatactg ctaaagcaga
    10141 tgccgttctt ggaaaagcag gcgttgctaa agaagatgta gcagcaaaca ataatatcac
    10201 aaaagaaact aatgtccttg ttctttgtgc aggtggaggt acaagtggct tgcttgccaa
    10261 tgccttgaac aaagcagcag cagaatacaa tgttccagtc aaagcggcag cgggtagtta
    10321 cggtgctcac cgtgaaatgt tgccagagtt tgacttggtt atcttggctc cacaggtcgc
    10381 ttcaaacttc gatgatatga aggctgaaac ggataaattg ggcattaaac ttgcaaaaac
    10441 tgaaggagct caatatatca aattgacacg tgatggacaa ggtgctcttg catttgttca
    10501 acaacagttt gattaataaa tttgtaaaag ttatttgaga cgagattatt ctttcaattc
    10561 cttataatat tgttaaccat atcattgcat ttcatttcct tggtttccgg aactcagaat
    10621 atcgtctcaa atgctttttt tgaaaggatt tatagttatg actaaaacac ttcctaaaga
    10681 ttttattttt ggtggtgcta cagctgctta tcaagcagaa ggtgcgactc acacggatgg
    10741 taaaggccca gttgcatggg ataagtatct tgaagacaat tattggtaca cagcagagcc
    10801 tgcaagtgat ttttaccata aatatcctgt tgatttagaa ttagctgaaa aatatggcgt
    10861 taatggtatt cgtatttcaa ttgcctggtc tcgtattttc ccaactggct atggcgaagt
    10921 aaatgaaaaa ggtgttgaat tctaccataa tctttttgcc gagtgccaca aacgtcatgt
    10981 tgagccattc gtgactttgc accactttga cacaccagaa gttcttcact cagatggaga
    11041 tttcttaaac cgcgaaaata tagaacactt tgtagattat gccgctttct gttttgaaga
    11101 gttcccagaa gtaaactact ggacaacttt caatgaaatt ggcccaattg gagatggtca
    11161 atacttggtt ggtaaattcc ctccaggcat tcaatatgat cttgccaaag tcttccaatc
    11221 acaccacaat atgatggttt ctcatgcacg tgctgtgaaa ttgtataaag ataaaggtta
    11281 taaaggcgaa attggtgtag tacatgcgtt gccaaccaaa tacccttatg atccagaaaa
    11341 tccagcagat gttcgtgccg ctgagttaga agatatcatc cataacaaat ttatcttgga
    11401 tgcaacctac cttggacact attcagataa aacgatggaa ggggtcaacc acatcctagc
    11461 tgaaaatggt ggagaacttg atcttcgtga tgaagacttc caagcacttg aagcagctaa
    11521 agatttgaac gatttccttg gtatcaacta ctacatgagt gattggatgc aagcctttga
    11581 tggtgagact gaaatcattc acaatggtaa gggtgaaaaa ggaagttcta agtaccagat
    11641 taaaggtgtt ggtcgccgaa tcgcaccaga ttatgtgcct agaacggatt gggactggat
    11701 tatttatcct gaaggcttgt atgaccaaat catgcgagtg aaaaatgatt atccaaacta
    11761 caagaagatt tacatcactg aaaacggtct tggatacaaa gatgagtttg tagataatac
    11821 cgtttatgat gatggtcgta ttgattacgt gaagaaacac ttggaagttt tatcagacgc
    11881 aattgcggat ggtgcaaatg ttaaaggtta cttcatctgg tcacttatgg atgtcttctc
    11941 atggtcaaac ggatacgaaa aacgttatgg actattctat gtagactttg acacgcaaga
    12001 acgctatcct aagaaatcag ctcattggta taagaaagta gcagaaactc aagtgataga
    12061 gtaatattac ttgaaaatgt aaaaaattgt tatccgatac tttagaaaaa gtacaacact
    12121 tatttagacc aggctttgaa aaggtatata ttgttagttt cgaagattgt cctatgattc
    12181 ctgagcttga agctaccccg ttgctaaaat gtggtaaatg gtatgtttca actggaaaag
    12241 agtggatttg ccattctgat ttagagttat cagcatttga atgggaattt ttacaatcac
    12301 tcgatgtaga aatacgtgag actattcact tcgaggtaaa ttatttgcct ttccaataat
    12361 aaaaagaaag gaattagttg tgactatcga attaaaaaac gagtatctta ctgttcagtt
    12421 taaaacactt ggtggtcaac tgacttcgat taaagacaag gatgggatag agtatctctg
    12481 gcaagctgat ccaaattatt ggaatggaca ggcgcctatt ctatttccta tctgtggtag
    12541 tttacgaaat gattgggcta tttatagacc tcaagaaaga ccctttttta caggtcttat
    12601 tcgtagacat ggttttgttc gaaaagaaga atttattctg gaagaagtca atgataatag
    12661 cgtgaccttt agtattaaac caaatgctga gatgcttgat aattacctat atcagtttga
    12721 actgagagtt gtttatacct tgaatggtaa atcaattaga accgaatttc aggtaacgaa
    12781 cttggaaatg gaaaaaacaa tgccttactt tattggagcc catccagcat tcaatagtcc
    12841 tttagtagaa ggtgaaaact atgaggatta ttatattgaa tttagtgagg ttgagtcttg
    12901 ctcgatacca aagagttttc ctgagacagg attattagat ttgcaagatc ggacaccttt
    12961 tttggaaaat caaaaaattc tcgatttgga ctactcactt ttttcccatg atgccattac
    13021 acttgaccag ctcaagtctc gtagcgtaac gcttcggtca aggaagtctg gtaaaggtct
    13081 tcgagtagat tttgatgatt ttccgaactt gattctgtgg tctacaacta ataaaagtcc
    13141 attcattgct ttggaacctt ggagtggtct ctccacttca cttgaagaag gtaatttctt
    13201 agaagataaa agacaggtaa cgaaaatttc acctcaagaa acttctagaa aaatctatga
    13261 tattactatc cttaattaaa atttgttatt atttgagtct tcaataagat ttgaatactc
    13321 ttaccttgtg atgatagatt ggtcttctcc gtgccagtct tacatcgcaa ggtttttaag
    13381 ttttttgtgg gtctatgtca actgtagtgg gtgacgaaaa gctaatatct aaagacagtc
    13441 tggtagtaaa aaataagatt atttactact tcttgattta aaataatagt ggagccatta
    13501 atttgtggca aatttaattt aatattttgc ctcaaattag ccacactttt tatagaacta
    13561 agaggaaaaa tgaaattttc ttcctattaa tatagttgtt tgaaatctta aatatagcgt
//
LOCUS       DACDBG010000069        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDBG010000069 DACDBG010000000
VERSION     DACDBG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.069"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IVZ90_002073"
     CDS             complement(<1..914)
                     /locus_tag="IVZ90_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8263104.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IVZ90_002074"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IVZ90_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8263105.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IVZ90_002075"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IVZ90_002075"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8263106.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IVZ90_002076"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IVZ90_002076"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8263107.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IVZ90_002077"
     CDS             4440..5321
                     /locus_tag="IVZ90_002077"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8263108.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IVZ90_002078"
     CDS             5318..6034
                     /locus_tag="IVZ90_002078"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8263109.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IVZ90_002079"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IVZ90_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8263110.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IVZ90_002080"
     CDS             6751..7752
                     /locus_tag="IVZ90_002080"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8263111.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IVZ90_002081"
     CDS             7749..9110
                     /locus_tag="IVZ90_002081"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8263112.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IVZ90_002082"
     CDS             9070..10221
                     /locus_tag="IVZ90_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8263113.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IVZ90_002083"
     CDS             10196..11365
                     /locus_tag="IVZ90_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8263114.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IVZ90_002084"
     CDS             11540..12919
                     /locus_tag="IVZ90_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8263115.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IVZ90_002085"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IVZ90_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDBG010000070        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDBG010000070 DACDBG010000000
VERSION     DACDBG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.070"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IVZ90_002086"
     CDS             complement(14..706)
                     /locus_tag="IVZ90_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8263116.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVZ90_002087"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVZ90_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVZ90_002088"
     CDS             complement(1143..1712)
                     /locus_tag="IVZ90_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8263117.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVZ90_002089"
     CDS             complement(1786..2109)
                     /locus_tag="IVZ90_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263118.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVZ90_002090"
     CDS             complement(2263..3075)
                     /locus_tag="IVZ90_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263119.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVZ90_002091"
     CDS             3429..4277
                     /locus_tag="IVZ90_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8263120.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVZ90_002092"
     CDS             4422..5363
                     /locus_tag="IVZ90_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8263121.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVZ90_002093"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVZ90_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IVZ90_002094"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IVZ90_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8263122.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IVZ90_002095"
     CDS             complement(9827..12013)
                     /locus_tag="IVZ90_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8263123.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IVZ90_002096"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IVZ90_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8263124.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IVZ90_002097"
     CDS             complement(12235..12675)
                     /locus_tag="IVZ90_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8263125.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IVZ90_002098"
     CDS             complement(12749..13288)
                     /locus_tag="IVZ90_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8263126.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDBG010000071        13304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDBG010000071 DACDBG010000000
VERSION     DACDBG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.071"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..314)
                     /locus_tag="IVZ90_002099"
     CDS             complement(<1..314)
                     /locus_tag="IVZ90_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAP8263127.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FK"
     gene            complement(311..2500)
                     /locus_tag="IVZ90_002100"
     CDS             complement(311..2500)
                     /locus_tag="IVZ90_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8263128.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(2634..3824)
                     /locus_tag="IVZ90_002101"
     CDS             complement(2634..3824)
                     /locus_tag="IVZ90_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP8263129.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(4696..6327)
                     /locus_tag="IVZ90_002102"
     CDS             complement(4696..6327)
                     /locus_tag="IVZ90_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8263130.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(6341..8014)
                     /locus_tag="IVZ90_002103"
     CDS             complement(6341..8014)
                     /locus_tag="IVZ90_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8263131.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8027..8344)
                     /locus_tag="IVZ90_002104"
     CDS             complement(8027..8344)
                     /locus_tag="IVZ90_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8263132.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(8369..9769)
                     /gene="lacG"
                     /locus_tag="IVZ90_002105"
     CDS             complement(8369..9769)
                     /gene="lacG"
                     /locus_tag="IVZ90_002105"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8263133.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10006..10791
                     /locus_tag="IVZ90_002106"
     CDS             10006..10791
                     /locus_tag="IVZ90_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8263134.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10794..11486
                     /locus_tag="IVZ90_002107"
     CDS             10794..11486
                     /locus_tag="IVZ90_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8263135.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11760..12131)
                     /locus_tag="IVZ90_002108"
     CDS             complement(11760..12131)
                     /locus_tag="IVZ90_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8263136.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(12531..12761)
                     /locus_tag="IVZ90_002109"
     CDS             complement(12531..12761)
                     /locus_tag="IVZ90_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263137.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(12855..13193)
                     /locus_tag="IVZ90_002110"
     CDS             complement(12855..13193)
                     /locus_tag="IVZ90_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263138.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         4098 a         2745 c         2165 g         4296 t
ORIGIN      
        1 attttaaaag gataaataat aaattcaaga caaaactgat gtattgtccc cacccaaata
       61 ttaatatcat catctatact aagtaaactt aggcgttcct taatttcttc agcagctcta
      121 ttagtgtatg tgatagcaat tattcttttt agtgatccat cgtgtttaat tgcttggtaa
      181 gcaattttcc ttgtaagtac ccttgttttc ccactcccag gacatgcatt tacataaagg
      241 ttatcttcac tttccacaac atctttttgt tctaaattta aatcatttac gattttttcg
      301 aagatatttg tcattttgaa acctcagcat aaagtatttt tgtgtaattt tctatctgat
      361 tgattagata cacaacatcc tcatctttac caaagtactc taataaagaa attattgcta
      421 tattcaaatc agctgtatca cttgttttta aactagctga taaagctgac aattctgtag
      481 tttcctcgta caagtcgatt gtatggagta gaattttttt ataaattgag agatctacta
      541 tttcctgaca tgcaaaagca atagcattaa gaatatactt aggaattttg attaaaaagt
      601 caattttttc acctaactca attgcaaacc accccttacc aacatgattg gctagtttta
      661 acatcgctaa atttctttct aaatattgtt cactttgtat ttcttcttta taagcagtta
      721 ttgttttttt atctttgaat gcgtcatcta taattggact gacataagtt agaatatttt
      781 ctttattcac atctaaaaac tcaatttcaa atgttgtatt agcataaaaa caattaaccc
      841 aattgttttc attaaaaagg gaggataatt tttcttttct tgcttgacct ttctcttgtg
      901 cagtgctttt atacaatcga tgttctgatg ggattgcctg ttcatctaaa tcagttacaa
      961 tagcacactt tctctgtatt ctatcatcgt caaacaagct agcgatatat tcaaacgaag
     1021 tactaccaac attaatcaaa cctattccaa gttcgtctaa gtttatacca aataattttt
     1081 ttactatttg aggaataagt atttcttcag catctccttc aactaagata acacctttag
     1141 aaaaaagcaa tccatttctt ttaacatcta aatatctctc tactgcgttt attagattta
     1201 gattgttctt ttttaacttt tctttagcaa atgagttcag attatttgct ggattcataa
     1261 cgatagattt gccattaaac gattttaata cattcatccg agatatttca gacacttcag
     1321 ctaaatgtac agaatgtgtg gtcatgagaa tctgagtata gtttttttga agatttaatc
     1381 cttcgaacag agttttttga atatgatgat gaatatgagc ttctggttct tcaatcaaca
     1441 taatattaag tagttctctt gaacgacacg cttcaaattc gactattttc aaagctaaat
     1501 aaatcatatt tagatgtcct aaccctagta agtctaagtc gatattttgt tctggcttca
     1561 ttgaaagaaa ttttgctaaa gaacccatat catctgacat agttgaggaa agaagaagat
     1621 taggagaata cactgaacca attatgctat ttagttgccg attaatattt tcgccaattt
     1681 tcctaatttc aggaacactg gtaatagtct catttaattg ctgtataatt gtttttaatg
     1741 actcaacatt atctgatgat atttttgatt caattgtttc catgattctt ttgactgggt
     1801 ttcgattgtt tttcatttct ctaagaacat ctcgaagagc gtcaacataa acaaccgaaa
     1861 tatgcttgaa aatatctgac atgtcaattc ttgagcccaa cagagcttca tcgtcttctt
     1921 caggattact agctatattg tcattgagtc ttcctaccaa tttatcataa ttttcattga
     1981 tacagaaatt tagatttgat ttagatgtgt agtaaaattc atagtcggat agacgaatag
     2041 aatttctaaa ttcgttaaat tgatgagagt ctaattcagt ggaaatatca aataattgct
     2101 ttctaatagc tttgttaggt ctaataaata gagaaatact acctacacct ttatctgcat
     2161 ttgaaatcaa ttcttctaag attgtttgac tttcagaatt taaagaaatg gaagcacaaa
     2221 tctcattgtc aaattcttct tcgctgattc cttcaaatgt cgcagagata ataatccaat
     2281 gcccttgcca cccattttga attccatgaa aaaaatctga ttcttttagt gttttactac
     2341 tatagtaata actatcatct aataagattc tcaatgcagt aagagcattg gttttccctg
     2401 aatcattctc acctataata gtatttgtct cattatcgaa tcgaaagatt tcggattcat
     2461 aatttctaaa atttcgaatt tgaagagtag atataaacat aagcagatct cctaactatt
     2521 taattaatat gattatatag taagtttatc ataaattatg ttagtaaaag ttctcattat
     2581 aagggagcgt caataatttt gtgtaaatga ttcttcccta ctgcagattg tttctaattc
     2641 tcaatcagtt ggcttagcat ttgttcgagc tcgccttcgg cctgttggaa acctttatga
     2701 ctgcgaccta ggaatttttg gttgtaagta tcaaaagacg agactaagaa acgttccatc
     2761 gactcttcat tttggaactg ttccttgcgg tggctgtatt tcttgatttg tttattaaag
     2821 gattcaatca agttcgtaga ataaatactc ttgcgtatgg ccaaggggaa atcatagaaa
     2881 gtgagcaagt gatcgttcga aagaatcgat tcaaccactt ttggatagct ggttttccat
     2941 ttttccgcaa aagtaccacg tgcttccaac gccacctctt tagatgaggc ttgatacacc
     3001 attttaaaat catcacagac ttccttacga tcatcgacac gcactttatg actgatatta
     3061 cgggaaacgt gtacacaaca atgttgaaaa cgagctttgg gatagaaccg actaatcgct
     3121 cctaccatcc cctttaagcc atccgtgatg aacaggagga catttttcaa accgcgctct
     3181 tgaaggtcca ataaaatttc ctcccaaatc gtgatggatt cagtcggtgc aatcgcatag
     3241 ctcaatactt cctttgatcc gtccgggcga atgccaactg cgatatgaat agcttccttg
     3301 gcgacggttt tccgctttaa cggaatatac gttgcgtcca tataaatagc agcataacgg
     3361 tcatgaagct cccgcccatt aaacgccgtt acctcttctg taaatgattt tgttatattg
     3421 gacatggttt ggggcgtgta gtaatgccca tacattttct caatcaagtc tgcgatttcc
     3481 gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaacgt gtcattcgtc
     3541 cgtctataag caggaacagt ctgttgcttg aactcaccat tgcggtcgcg cggaatctgg
     3601 agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt acgagaatta
     3661 cccgtgttaa aaccaatgcg atcatacttt tcgtaatcta ggaaagccgt taactccgtt
     3721 ttgagaagcg tattgactgc tttctccaaa tggacacgga ataattcatt caaatcgcct
     3781 ttgttcgcta gagtctttag aatttctgta gtaaaatcgt tcatagggaa gtcctctctt
     3841 ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt ctatttacac
     3901 aaaatatttt acactctcca ttataacaat tgggttaaaa agaaaataga tagtattcta
     3961 cctcatattt atttgaaagt aatatataaa agacgttcaa attcgaacgc ctctcgttaa
     4021 ccaaatattg gattcagaat ctagttttca tatataaaca gagtatgatt aggtgcaaaa
     4081 attgcacttt tattgcactt ttatttttat ttatagtgtt ttactgttct gtatattgtt
     4141 ttaactccct tgatagtatc gcattctgtt ttatacactt tacgttgaaa acagagtggg
     4201 aataaagagc caacataata tggaggggga atccctataa atcaacattc ttcaatagct
     4261 aaaatgcttg attttcatta aaaataagat ggttccctac caaaagagac tataatataa
     4321 gttaatttgt ttcattctta tcataaaaag aagttctcga aaaacgaact gttttccgag
     4381 aacttttttc tatccatcta agtgctcaaa tcatattcac gacggaattc taaggttacc
     4441 ctttcttaaa caaactttat gttatctgca caactttttg caattttttc attgcttctt
     4501 acgaaatcgt tgaactctct ttagcataat acaaagtgta gatgaagtct ggtgctttac
     4561 tcgttttggt aaaaatgact ggaaatcgtt tctggttatt tttggcaaag gcgaggtgca
     4621 ggttcgattc ctgccttcta cgctaacgat gtggctgaag ggaacctccg atcttgaatc
     4681 gaaacgatcc gcagtctatt gtttcaccaa tgaaacaaaa tcaacgttcg tccaagcatt
     4741 tcctgaagcc ttgctataaa ccccaatttc acaaacacct gttgtaattg gaatgtcttt
     4801 gattgtaatt tttgtccatt cacttgtttt aacaaccgca gtatttagtt ccgctccacc
     4861 ataatttttg atatataact gattagtatc aaactctccc ccagacatta cataggcact
     4921 caatgtgtaa gttcctttgc ttatgccact aacattttga taagctgatg cctcgtatgc
     4981 ttgatcctgc caaaaagtta gtttgtagtc cccaaaaaaa gtgcccgctt ccgttttgat
     5041 cgtaccctta gcggttcctt gttgaaacca ggtttgccaa ttggaaggac tggtcgttgg
     5101 tccatcgctt tcgaaacttg catttttaac tagatttatt tgcgcccctg cttgtttaaa
     5161 cgcacgcaag cttggcaagg cgtttccagc aaaatcaaac atcgcttggt tgtcccattc
     5221 gtttcccata gtggattgaa cactgagata attcatacct gcttgtgttg cccaactcac
     5281 atttccgttg taccaaagag gttcccaata gaagaagccc gccccgcgat tattcggtac
     5341 tgcctgaatc cgatcggcaa gatccagcaa atactctgct tgtcctgtcg gtgttggttc
     5401 atatcccccc gcagctgctt cggcttcacc aaaagcattt ggcatagtat cggcattcgc
     5461 tgttgtatgc ccataagctg tttcaactac gatcacttct ttaccatacc gttggctgat
     5521 cgcattcata ttggtttgaa gttcattcat tgaaccgtgc caatacggat aatacgacac
     5581 tccaattaaa tcaaatgaca cacctcgact cgtgatttca tcaaaccacc aagtaaatgc
     5641 tcctgtatcg ccaccatcag ctaagtggag cattactgga gttgttttag gttgagtgtc
     5701 tcgaacagct tggataccag acttcaaaaa agcagccaat cgatcaaatt ctcccccgcc
     5761 ttctccccaa gacttgccat aaggccagag cattccgcta ttcagttcat tgccgatctg
     5821 aaccatatca ggataaacgt ccagctgctt catttccgac agaacgttct ttgtatagtc
     5881 gtacaatgcc gtattcattt cttcaaaggt cagaccttgc catgctttag gaagattctg
     5941 tttcccagga tcaacccaaa aatcactgta gtgaaaatcc agcagcacct taagtcccag
     6001 attttttgct cgttttgcaa ggtcaatcgt cgtttccaga tcatttgtcc ctgctccata
     6061 actttctcca ttactattat aaggatcctg ccacaaacgc agtcgaacat agttgacacc
     6121 attatttttt aaaatctcta aggcatcttt tgaaatatcg tcttcataat attttgcacc
     6181 agatttttcc atatctgaca ggatcgaaat atctgctccg cgaataaatt cgctggcaga
     6241 tgcttcaaca gtttttgaaa cagcccccaa agcccctaac acagctgcaa ataaaacagc
     6301 tttacgcaac caaattccca ttttcattat ggctcctcct ttattgaaat tggttcagaa
     6361 taaactgaac agctttttca ggatctctcg ttaatccgat atattctgct ccttttgtcg
     6421 caaccagctt gattcctaag cgatcggtgt cttctttgat atcatcatag taagaattga
     6481 cttgcggtgc taagaccacc atatcaaaat cctgcagcat ttcgtaatga gaaccataag
     6541 cacccgccat tgcactgatc ggtgctttcg attcagatgc accttttttt aaagcattag
     6601 ctaacatcgc acttgtccct gcgccagcac aaagtaccaa cacattgatt tctttatttg
     6661 catcgctgat aacttgactc gctgccacag gtacttcctc acgtacatcg gcagaagcac
     6721 cttcctcggt tgcgatcacc tctgcttttt gtgcttcttt ctctactaag acagcatcgt
     6781 atgctttgca aaatggtaga tagatcagga agtcaacgac gattagaacc gctgctagaa
     6841 caaaagctaa gatacccatg cccgttccta gaaccaaacc gatcggtgct ggagttgccc
     6901 aaggcaaaac gtacataaag ctgttcatgc ccaatacatc gacaaaaaac ttgaacaggc
     6961 aaacgtttac aacaggtgtt aacaaaaatg gaataaaaaa ataagggttc aaaatcatgg
     7021 gtgccgcaaa tagcaatggt tcgttgacag cgaaacttac tggaataaag gaagctttcc
     7081 caactgcctt caactgctta gacttagcaa agagtaggaa gataaaagga acaaccaatg
     7141 ttgccccagt tcctcccata gttcctacaa aattccctaa acccaccgtc agtactttag
     7201 aagcttgttc accggaattg aacaattgca gattggtttc cacattggca tagataattg
     7261 ccgcaattgc cggctccacg atcgacggtc catgaacacc gacaaaccaa aacagcgcca
     7321 tcgctcccca gataatagcg attcctaagt acccgtcagc tgctgtaaat agcggctgca
     7381 gcaccgtaat gattgcttct gcaaatgaga tttggaagat ttgtcgaatg cctaaatcaa
     7441 ttaaaacggc cgcaaatact gaaaaagaga aaggaaaaat atctcggaag gtttgcgaaa
     7501 tcgttccagg aacttcttta ggcattttaa tggtaatatc tttcagcaca caaaacttat
     7561 ataaattaac tgttaaaaat gcagcgacaa aggaagccag tagtcccttt gtccccatat
     7621 attccgttga aaatccacct tcaatcggag agacgcttaa caacaggaaa cttaccacag
     7681 aggccagcat cacagaggtg gtgttgatta ctttgttttt cggtaagcga cgattcatcg
     7741 aatcagccaa gcagcgagca gtggttgctg aaacgagtaa ccctacaaca cccatcgaat
     7801 aattatagac tttccatagc catgtggaga cagattcagg ccattggtaa ccaaaaatct
     7861 caggaaccgc agcaatcaaa atgaaaatgc ttgaaaataa aatagctggc atcgctgtta
     7921 aaaatccgtc acgaacggct cctaaataac tattacgtga gattttttca aaaaatggtt
     7981 tccctttttc gatttgttta atgattccat ccatagcgtt cttctcctat ccttgattct
     8041 gtctgtacag tacaataaag tcttgaatca gatctctcag caataaagtg gtcattaaat
     8101 gatcctgccc atggataaaa atgaatccca tctccatctc tttcccagat gcttcttccg
     8161 ctaaaatttt tgtttgcgaa ttatgtgcaa gggttaaatt ttcgtcggct tctttcaacg
     8221 ccgaatcaac atgttcaaag tttccttgtc ttacttcttt caataatttc aataatgtac
     8281 tgcgagcgtc gcctgaatat gcaacgatct caaaaccaag catttgtaat tcctctttat
     8341 tcatcttttc ttcctccatt atcacttatt aaataatcgt tttggtttca ctgaccaact
     8401 tgtaccagta agctgattct tttgggtaac gcttctgggt ttcaaaatca acataaaaca
     8461 agccatacct tttgttatac ccatttgtcc atgagaaaag atccataagc gaccacaaaa
     8521 aatacccctt cacatttacc ccggctgtta tcgccttgct taaggattcc aaatagactc
     8581 tcaaatagtc gattctcggc tgatccataa taatgccatc ttcaaactga tctttatacc
     8641 ccatcccgtt ctcagtgata tagattttgt tgtaatgtgg gtaatcgctt ttaattcgca
     8701 gcaacaagtc atatagacct tctggataaa tcagccagtc ccaatcagtt cgaggaattc
     8761 cttctttata aatacgttct ccgattccct tcactttata aacagaggtc cctttttcac
     8821 ccgtaccgtt atgatggatt gcattttctc catcgtaagc tttgacaaaa tggcattggt
     8881 agtgattgat ccctaagtag tcattacgcg tggatgcttt tttcaattca acgaaatctt
     8941 cttcaggaaa atgatagctg gcctgatttg cttcacatat ctcatcaaga gcagtcaagg
     9001 tttcagttga ataatagcct aaataggtag catctaataa gaaacggatc gacaatgcat
     9061 cgtctaaaaa ggcagcatgc ttatcttccg gcgcgtctgt tgccgcatat ttcgtctcta
     9121 atgagtgaac tacaccaatt tcacccggaa gttcgttttc tttgaaatag ttcactacac
     9181 gggcatgagc aaccatcata ttatgaagac aggcgacgat ctttgtaaaa tcatatttga
     9241 ttcctggtgg gaaaacacct aacaaatatt gattggttgc aactggatag atttcattaa
     9301 aggtactcca caccttgact tctttgaatt catgaaaaca aaaaatggca taggaaacaa
     9361 atgcttctat tgtttctcga ttcaaaaaat ctccatggtc aaacaaacgt ttaggggtat
     9421 caaaatgatg caacgtcaca aacggcgtta cgtttctttt cttacattct tcgaacacac
     9481 gatgataaaa agcgattcct tctggatttg gtttcccagc tccatcagga aaaatgcggc
     9541 tccaagcaat ggataatctt agaccattta caccaaaacg ttcacacaat tcaatgtcct
     9601 ttgcatattg atgataaaaa tcacttgccg gatcgggact gaatcgccct tgttcctcta
     9661 aaaagtcatc ccaagcaaca gcgcctttgc caccttcctt agtggctcct tcaacctgat
     9721 aggctgctgt ggctccgcca aaaataaaat cttctggtaa tttaagcatg tacatctctc
     9781 ctcaaatcta taatcagttt tcgctctttc taactgattg cattgtaaac gcatccaaaa
     9841 catttgtcaa caaaaacaaa cacaaataat cataaaaacc agaaaacagc aataaaatct
     9901 attgtaacaa acataaaaac caccctaaat gtttactttt gttcgaatta gattgactgt
     9961 tgtccgttta tatgatagat tatccttaaa ttagaggtga agacaatgtt gaaaaaagaa
    10021 agattgctta ctatcgtaga aatggtcaat aaaaaaggaa tccttaccgt taatgaaatc
    10081 atcaatcaat tagatgtttc tgatatgacc gttcgcagag atttagatga attagaaaaa
    10141 tcgggcaagc tgctgcgtgt ccatggaggg gctcaaagtc tttcctacac tttagatcaa
    10201 gaactttctc acactgaaaa agccactctg cagattcaag aaaaaaaaga aattgctgcc
    10261 acagcagcct ccttgattca cgagggagaa acgattttct taggaccagg tacaacaatc
    10321 gaactattgg cgacgcattt actgaacaag aaaattcgga tcattacaaa taattatcca
    10381 gtttttgata ttcttgctca agcagatgga ccagaaattc tactgattgg cggtgatttc
    10441 cggaaaaata ccggcgcatt tgtcggaccg atcaccaacg acaatttaag aagactaaag
    10501 tttacaaaag catttatcag ttcaaatggc gttcacaacg aagcgatctc tacttacagt
    10561 accgaagaag gagaagcaca gcagatcgct ctaaataatt cccgtaagaa atatctactc
    10621 gttgataata aaaaattcaa tcgagatgat ttttatgtgt tttataattt gcatgatttc
    10681 gatctgctca ttacggatcg ccagatctcg cgggaggttc aagagcacta tgcacaatat
    10741 gtcgatctgc aaattgctga ttcaaccgaa aataccacta taggagcgta actatgaaag
    10801 ggattatatt tgatttcaac ggaacaatgt ttttggactc tcatctgcat gaagctgctt
    10861 ggctgcatat gatacatgat catacaaaag atggtttatc agacgaggat attttaaaaa
    10921 atattcatgg tcggacgaat acggagattt tgaaacattt catttcagag catttgaccg
    10981 cctctgagat cgccgtattg tcagaagaaa aagaaagcta ttatcgtatg ctttgcttgc
    11041 aaaatgaaaa cgagcttcag ttgacaaaag ggcttgaagc gactcttgac cagctgactg
    11101 accaacagat tccattaacg attgccacgg ctactgtcaa agagaatgtt gctttttatt
    11161 ttgatatttt cgatttagcc cgctggtttg actgggacaa gattgtctat gacgacggct
    11221 ctttccctgg gaaaccgcaa ccggatattt ttcttaaagc ggcagacaaa ctgagcttag
    11281 ctccggctga ttgtcttgtt atcgaagatg cttactcagg attacttgct gccaaacgtg
    11341 cggggatcgg aacaattatt gcaattgatc cattcggtaa aaacaaaaca gtatttgaac
    11401 aggcacagct aaataaagat ggaaccatca aagattttac tggtttttgg acagagcacc
    11461 tgtctgccac atccgttaat tcttaaacga ttcaactaac taccaaagaa aagcaactga
    11521 aatgcacacc tccttttata aaaaactgcc agtgaaacga catgaaagat acactgctat
    11581 atccttagtg aaatatagca gtgtatcttt gactttttca ttcttttaac catatgacgc
    11641 gttcatctct atctataatt aacattaaaa atagtgtttt cacattattt ctcaaaccaa
    11701 tcttaaaaat catcaggagt acttttttga tatactttta ttaatgctgg gcgtaatggt
    11761 taaaagattt ccactagttc ctctccacca agctgagttg aaaaatgctt atctgtaaac
    11821 aacatagaca aagaggaaat aaacgtttcc gtatctttct ctgtcccaag gatattaatt
    11881 aattttccta ctaggtaagt atcattaaac ttaagatctg ccttcacatc aaaagttttg
    11941 ataaaagctt ttatcatgcg tacaattttg tctttatcgc cataattgat ttcaccactc
    12001 atatcaatat agtcactaat ccctgaagaa ataatggcaa ctgcttcttc tttcgcctta
    12061 tcagataatt catttaatga ctgggattga ttgatgactt cttgagtttt atctgttacg
    12121 taatctctca ttatcctatc ggcatactcc gcaaagtaat cttgaaaaaa ttcttctaca
    12181 gggtaaccaa cttcgttttc ccaaccagga gaaatttcat tatcagtact tattaaatct
    12241 attaaaaacc aacccgttct ctgagtgttg attctataat atttttaatt tcctatataa
    12301 atcaaaaata ttatagaatc aataagctaa ttaagaaaca aactttagaa actctagaag
    12361 aacttctgaa tttatataga agtgtgtagc tacttactaa atatacaaaa gttaaaataa
    12421 aatactctgg atactcttta tactgcatta aagttagtaa tattaacagc aaagatatcg
    12481 aaaaaacaat aacataatat gtgtcaaaat ttttcataat aagaactccc ttactgttgc
    12541 atactaacat aatccaaaaa ctcttctact tcagctaaag acattccact ttctttagtc
    12601 caaaaaacag ttttttctat cgatccttct ctgtaaactg taaatgtcgg agtcgagtta
    12661 attgagtagc gactcttgaa ttctttccac tcattttcat cttctctaac ttttgctaca
    12721 ttcacgtaat aaatattttc ttttcccttt tctaccagca tatcgaatag cttcctgctt
    12781 ttgatgatcg atttcttgtg ctacatctag taaattttca tcaatttcta tatcataaag
    12841 tatttcacta gtgtctacat ccacaatata tactttttat cagtagcaat ttttgagtaa
    12901 tccgttaaaa tgatctcagt ctcgtcactt tcatcagtat aaatatacgt taacaatgaa
    12961 ctcacctttt caatcggtgt tagctctaag cctactgtat tttttactat tgagtcatta
    13021 tttaccacca taacttcgta ctgtatatac catcctattt ggtttccatc aatatcagta
    13081 attggctcac tagctaatgg agtctccgga acaatatttt cctgatattt cttaaactct
    13141 tctgtagcac gaaaaaggtt atcatgttcc tcacttgaaa ctggttgagt catagaaaat
    13201 gttacttggc taaacagagg gaaaataaac actacaatca acaaagtata aaaaaaagcc
    13261 cttctttttc attttttatc ttccaatcac aaattgaaaa actt
//
LOCUS       DACDBG010000072        13281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDBG010000072 DACDBG010000000
VERSION     DACDBG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.072"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..98
                     /locus_tag="IVZ90_002111"
     CDS             <1..98
                     /locus_tag="IVZ90_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8263139.1"
                     /translation="SVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(160..1971)
                     /locus_tag="IVZ90_002112"
     CDS             complement(160..1971)
                     /locus_tag="IVZ90_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8263140.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1968..2333)
                     /locus_tag="IVZ90_002113"
     CDS             complement(1968..2333)
                     /locus_tag="IVZ90_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8263141.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2496..2891)
                     /locus_tag="IVZ90_002114"
     CDS             complement(2496..2891)
                     /locus_tag="IVZ90_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8263142.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2909..3871)
                     /locus_tag="IVZ90_002115"
     CDS             complement(2909..3871)
                     /locus_tag="IVZ90_002115"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8263143.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3871..4083)
                     /locus_tag="IVZ90_002116"
     CDS             complement(3871..4083)
                     /locus_tag="IVZ90_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8263144.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4104..4802)
                     /locus_tag="IVZ90_002117"
     CDS             complement(4104..4802)
                     /locus_tag="IVZ90_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8263145.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4822..5364)
                     /locus_tag="IVZ90_002118"
     CDS             complement(4822..5364)
                     /locus_tag="IVZ90_002118"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8263146.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5496..6500)
                     /locus_tag="IVZ90_002119"
     CDS             complement(5496..6500)
                     /locus_tag="IVZ90_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8263147.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6497..7486)
                     /locus_tag="IVZ90_002120"
     CDS             complement(6497..7486)
                     /locus_tag="IVZ90_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8263148.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7483..8289)
                     /locus_tag="IVZ90_002121"
     CDS             complement(7483..8289)
                     /locus_tag="IVZ90_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263149.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8455..9411
                     /locus_tag="IVZ90_002122"
     CDS             8455..9411
                     /locus_tag="IVZ90_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8263150.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9488..10006)
                     /locus_tag="IVZ90_002123"
     CDS             complement(9488..10006)
                     /locus_tag="IVZ90_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8263151.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10094..10243)
                     /gene="rpmG"
                     /locus_tag="IVZ90_002124"
     CDS             complement(10094..10243)
                     /gene="rpmG"
                     /locus_tag="IVZ90_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8263152.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10471..10917
                     /locus_tag="IVZ90_002125"
     CDS             10471..10917
                     /locus_tag="IVZ90_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8263153.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11112..13007)
                     /gene="asnB"
                     /locus_tag="IVZ90_002126"
     CDS             complement(11112..13007)
                     /gene="asnB"
                     /locus_tag="IVZ90_002126"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8263154.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4139 a         2822 c         2257 g         4063 t
ORIGIN      
        1 cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa
       61 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
      121 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
      181 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
      241 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
      301 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
      361 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
      421 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
      481 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
      541 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
      601 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
      661 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
      721 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
      781 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
      841 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
      901 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
      961 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
     1021 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
     1081 caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
     1141 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
     1201 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
     1261 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
     1321 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
     1381 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
     1441 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
     1501 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
     1561 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
     1621 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
     1681 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
     1741 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
     1801 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
     1861 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
     1921 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
     1981 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
     2041 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
     2101 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
     2161 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
     2221 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
     2281 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
     2341 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
     2401 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
     2461 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
     2521 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
     2581 aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg
     2641 tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct
     2701 aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc
     2761 attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt
     2821 agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc
     2881 cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac
     2941 caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt
     3001 gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg
     3061 ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc
     3121 aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt
     3181 cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt
     3241 tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg
     3301 tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag
     3361 gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc
     3421 cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat
     3481 cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga
     3541 tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc
     3601 agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac
     3661 ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga
     3721 ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc
     3781 tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac
     3841 ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc
     3901 aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa
     3961 acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc
     4021 acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc
     4081 catctttaag cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa
     4141 atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa
     4201 tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc
     4261 ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac
     4321 acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga
     4381 aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc accaccagac
     4441 agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata
     4501 taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac
     4561 agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact
     4621 aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg
     4681 atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa
     4741 gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc
     4801 atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct
     4861 ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg
     4921 aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct
     4981 attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc
     5041 tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc
     5101 ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt
     5161 ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc
     5221 tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc
     5281 tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg
     5341 taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg
     5401 aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat aaaaagaaga
     5461 cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc gtgtttttgt
     5521 ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta
     5581 ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag cagaaaaatg
     5641 gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac gactagcttc
     5701 gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc tacactaccg
     5761 cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac gttaaggcct
     5821 aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc ataaagtcct
     5881 cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg ccagctcgca
     5941 ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt agataattgg
     6001 atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc ggacaataag
     6061 attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac acagcaagca
     6121 gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga atagtgcgaa
     6181 aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc caatatacta
     6241 gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac gccaagacaa
     6301 gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac aatcagcgcc
     6361 aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta aataaaaggc
     6421 gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa gaaatagaac
     6481 gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa aacggaacgc
     6541 caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg
     6601 taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt agaacataac
     6661 gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca ataaatgaaa
     6721 caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag aaaacaatca
     6781 accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc aaatgaatca
     6841 aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca
     6901 atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa aaagcaagat
     6961 cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa aaagaactaa
     7021 tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa aactgcaaaa
     7081 aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga aacacagaaa
     7141 aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa gcacccgcgt
     7201 tgattcctag taagcctgaa tctgctaaag gatttcttgt caccccttgc atcaatgcac
     7261 cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc aaacgaatat
     7321 ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat
     7381 ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa
     7441 caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt gatcctcctg
     7501 ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg gaagcgtgac
     7561 aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca cttcttcagg
     7621 tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg aaaaacgtga
     7681 agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt gattgatttc
     7741 agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg tcggttcatc
     7801 caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac gctgggtttg
     7861 tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg tagcatccaa
     7921 cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt tttgataagg
     7981 gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt cactagtttg
     8041 tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga tatcagagtt
     8101 attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt tcaataaagt
     8161 agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag gaatcgtaac
     8221 agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag ctgctcttaa
     8281 tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg ctgtttcaac
     8341 atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca gcgttcgaaa
     8401 tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa
     8461 ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt actgagcgct
     8521 tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac ttcatccaat
     8581 agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa cgcgcccaaa
     8641 cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa acctatcgcc
     8701 cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt aaaggatgtc
     8761 ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc tgatttgctg
     8821 ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg
     8881 ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact aaaagatgtc
     8941 ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc
     9001 atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc tgcagtgttg
     9061 tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta
     9121 tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa aaaagccact
     9181 tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga ctacatcatc
     9241 cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa gaatctaaaa
     9301 gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct atataacgga
     9361 cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg attttaacta
     9421 aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt cttattaaca aactagacgc
     9481 atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat ttttgaaaac
     9541 gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata agggcgaagt
     9601 ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg atcaatgttc
     9661 gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca cccgaaacat
     9721 agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa tgcattactt
     9781 ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata tggcgataag
     9841 gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa attcgatcca
     9901 ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac aaaccatttt
     9961 tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta tccgctcctt
    10021 atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca aggaattgag
    10081 aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat
    10141 atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt agatagttac
    10201 gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc cctcccattt
    10261 ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat tttttctaga
    10321 ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat acaacggttt
    10381 tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg tttatataga
    10441 ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga ctttcctgca
    10501 tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta
    10561 ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca agcaaaaaaa
    10621 tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc
    10681 aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc agatgtgatc
    10741 gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac
    10801 cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa
    10861 tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga ttattgaaca
    10921 tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg
    10981 ttcctctgcc aactccgaac cataagtcat aatatctata aaacgggaag agctgttttt
    11041 tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt tttattgata
    11101 tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat cgtttcatct
    11161 acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt tctttgctct
    11221 tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg atcaaaaaat
    11281 tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg ttcttctttc
    11341 aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca ttcttccgga
    11401 agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt caataaaaac
    11461 ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg tactcggact
    11521 tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt tggcatccat
    11581 tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc tgttttctcg
    11641 taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg tgttaacact
    11701 tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc ttttgcagga
    11761 gcaagtgatt catataaatg gatccgtcca tggaagtttt tcatttttcc gattttacgt
    11821 cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat cacacggatg
    11881 aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc atccgcccct
    11941 tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt
    12001 acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg gaatgctttg
    12061 aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag ttcggttagt
    12121 tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga gaaagaatga
    12181 tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa gaaagaacca
    12241 acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc gtcgattttt
    12301 tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc ccaatattgc
    12361 tgaatgtcta tttttccatc tttatacgta taataatggc cttcaggcaa acgatagaca
    12421 tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata aggtttcaac
    12481 gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt gatttctgaa
    12541 ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc aaagtggtct
    12601 cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa cattccgcgg
    12661 atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac ttctgtatct
    12721 gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg ctggtaatta
    12781 tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat aggctgagta
    12841 ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg tttatcgata
    12901 tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt attaataatt
    12961 gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta actttcacat
    13021 cctaatataa aattactgtt tatttcattc atatgcatct tatcatagaa taaatagcgg
    13081 acgaatattt tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt
    13141 ttacagaaaa taaagtccca atatctaatt taccatgttt tcttttttga ggctacctta
    13201 ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc aaaaaaacca gcaaaaaaag
    13261 acacgaccta aagatcgtgt c
//
LOCUS       DACDBG010000073        13262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDBG010000073 DACDBG010000000
VERSION     DACDBG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.073"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            165..1453
                     /locus_tag="IVZ90_002127"
                     /pseudo
     CDS             165..1453
                     /locus_tag="IVZ90_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1579..2658)
                     /locus_tag="IVZ90_002128"
     CDS             complement(1579..2658)
                     /locus_tag="IVZ90_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8263155.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2761..3201)
                     /locus_tag="IVZ90_002129"
     CDS             complement(2761..3201)
                     /locus_tag="IVZ90_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8263156.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3258..3668)
                     /locus_tag="IVZ90_002130"
     CDS             complement(3258..3668)
                     /locus_tag="IVZ90_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8263157.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3848..4282)
                     /locus_tag="IVZ90_002131"
     CDS             complement(3848..4282)
                     /locus_tag="IVZ90_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8263158.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4382..5230)
                     /locus_tag="IVZ90_002132"
     CDS             complement(4382..5230)
                     /locus_tag="IVZ90_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8263159.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5619..7385
                     /locus_tag="IVZ90_002133"
     CDS             5619..7385
                     /locus_tag="IVZ90_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8263160.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7487..8746
                     /locus_tag="IVZ90_002134"
     CDS             7487..8746
                     /locus_tag="IVZ90_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8263161.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8893..10194
                     /locus_tag="IVZ90_002135"
     CDS             8893..10194
                     /locus_tag="IVZ90_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8263162.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10197..11048
                     /locus_tag="IVZ90_002136"
     CDS             10197..11048
                     /locus_tag="IVZ90_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8263163.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11061..11876
                     /locus_tag="IVZ90_002137"
     CDS             11061..11876
                     /locus_tag="IVZ90_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8263164.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12027..13193
                     /locus_tag="IVZ90_002138"
     CDS             12027..13193
                     /locus_tag="IVZ90_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8263165.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4551 a         2390 c         2473 g         3848 t
ORIGIN      
        1 gcttcttgag tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata
       61 ttactgtttt tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac
      121 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
      181 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      241 cagaggagat agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      301 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
      361 cagcgaccat caaagcaaaa attgactatt caggagactt agcacctata ctacaatcag
      421 ctgttacatt tgagaatgtt gatcccgctt tgtcgattgg ctatgacgag aacactttga
      481 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
      541 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catcatttaa
      601 cgccgtatag tcagcggcag acaattgagc aagattcagc tatcgactca ggaggagatc
      661 ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag
      721 aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa
      781 ctcaaccaga gccagacaat gaatcaaaac ctgaaataac ccctggagaa aaaccagatg
      841 tagacccaga agaaaaacca gatgtgactc cagaaccaga tacggattct ggaaaccaaa
      901 cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg
      961 tagccccaga agaaaaacca gatgtaactc cagaaccaga tacggattct ggaaaccaaa
     1021 cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg
     1081 tagatccaga agaaaaacca gatgtgactc cagaaccaga tacggacgca agagaccaag
     1141 gaataccaga aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa
     1201 aatcaaatct agcaattcta aaaataaatg aagagcagct gaataaaaaa tcacgaatat
     1261 ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc
     1321 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
     1381 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
     1441 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
     1501 ccagaaaatg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
     1561 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
     1621 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
     1681 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
     1741 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
     1801 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
     1861 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
     1921 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
     1981 cgtatttaaa agagcgacaa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
     2041 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
     2101 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
     2161 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
     2221 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
     2281 aacaggatct gcatcttttg gctgatgctc gtaaaaatta ataacggact ggatcatgtc
     2341 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
     2401 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
     2461 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
     2521 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
     2581 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
     2641 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagca aaaagaaaaa
     2701 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
     2761 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
     2821 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
     2881 tgcttgtttt tgcaacgcag caccttgagc agtcagcgta acatttacga ttctttcatc
     2941 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
     3001 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
     3061 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
     3121 gggagtgtag tgtctaatga tttctttaga ggcggcatat aaaggaaaac atacttgatt
     3181 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
     3241 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
     3301 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
     3361 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
     3421 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
     3481 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
     3541 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
     3601 tcgggattat gcacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
     3661 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
     3721 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
     3781 cgcgctttct cgaatagcaa acatatacaa aaaatatcag agacaaaaaa taatctcatc
     3841 ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat
     3901 ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac
     3961 caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat
     4021 tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag
     4081 cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtaccat
     4141 acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta
     4201 tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat
     4261 cgatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga
     4321 ttcttttatt ataccagcta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa
     4381 ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa
     4441 tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat
     4501 cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta
     4561 gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat
     4621 gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga
     4681 caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaataa
     4741 acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt
     4801 ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt
     4861 tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc
     4921 gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa accaagcttc
     4981 cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa
     5041 cgatattcaa cactaaaata actatagctc ggtcaaagcc taaagcagct tccagtaata
     5101 ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag
     5161 cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg
     5221 tttctttcat ttattcactt ccttaattca ttaataatac tcttatctta ttataactta
     5281 cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt
     5341 ttcattcttc tagaatatac ttctacgcca aaaactgtta aaaaactttt actttagaaa
     5401 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagact
     5461 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
     5521 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgcttct ctatcactta
     5581 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
     5641 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
     5701 gtactaggaa aaatgatatc aaaaaagtat atgtgctatg tggagatcca tataccataa
     5761 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
     5821 atgattattg ggaaattgaa gtgacgagcg aaacaagtcg cttacagtat gcgttccatg
     5881 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
     5941 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
     6001 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
     6061 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
     6121 cagaccgaga agactttttc gggggagatt tgcaaggtgt gatggatcat ttggattact
     6181 tgacggacct tggcatcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
     6241 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
     6301 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
     6361 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
     6421 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
     6481 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
     6541 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
     6601 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
     6661 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
     6721 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
     6781 attatgcatt tacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
     6841 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
     6901 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
     6961 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
     7021 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
     7081 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
     7141 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtaaatg
     7201 atcgtgaaga taagctctat ttcacacgga aactagaggg acagatcatc tatgtttact
     7261 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa
     7321 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
     7381 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
     7441 ataataaggg tgtaacagca cgttgaatac aaaggaggaa atgaagatga aaaaaagaag
     7501 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
     7561 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
     7621 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa
     7681 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
     7741 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
     7801 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
     7861 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
     7921 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
     7981 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
     8041 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
     8101 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
     8161 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
     8221 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
     8281 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
     8341 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
     8401 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
     8461 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
     8521 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
     8581 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
     8641 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
     8701 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
     8761 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
     8821 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
     8881 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
     8941 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
     9001 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
     9061 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
     9121 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
     9181 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
     9241 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
     9301 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
     9361 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
     9421 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
     9481 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
     9541 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
     9601 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
     9661 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
     9721 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
     9781 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
     9841 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
     9901 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
     9961 gggaatttca ataatttctc catgatctat ctgtttaaca atggcggtcc cggaagcgta
    10021 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
    10081 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
    10141 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
    10201 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
    10261 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
    10321 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
    10381 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
    10441 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
    10501 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
    10561 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
    10621 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
    10681 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
    10741 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
    10801 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
    10861 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
    10921 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
    10981 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
    11041 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
    11101 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
    11161 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
    11221 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
    11281 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
    11341 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
    11401 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
    11461 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
    11521 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
    11581 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
    11641 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
    11701 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
    11761 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
    11821 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
    11881 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
    11941 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
    12001 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
    12061 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
    12121 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
    12181 agattacaag taacgcatct ttggcactag gagaagccta tatggatcat cgaatcgaga
    12241 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
    12301 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
    12361 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
    12421 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
    12481 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
    12541 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
    12601 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
    12661 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
    12721 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
    12781 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
    12841 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
    12901 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
    12961 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
    13021 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
    13081 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
    13141 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
    13201 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
    13261 ac
//
LOCUS       DACDBG010000074        12966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDBG010000074 DACDBG010000000
VERSION     DACDBG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.074"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IVZ90_002139"
     CDS             complement(287..649)
                     /locus_tag="IVZ90_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263166.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVZ90_002140"
     CDS             complement(723..1103)
                     /locus_tag="IVZ90_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8263167.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVZ90_002141"
     CDS             1185..2360
                     /locus_tag="IVZ90_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8263168.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IVZ90_002142"
     CDS             complement(2437..3021)
                     /locus_tag="IVZ90_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8263169.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IVZ90_002143"
     CDS             3222..4220
                     /locus_tag="IVZ90_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8263170.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IVZ90_002144"
     CDS             4332..4841
                     /locus_tag="IVZ90_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8263171.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IVZ90_002145"
     CDS             complement(4871..5530)
                     /locus_tag="IVZ90_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8263172.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IVZ90_002146"
     CDS             complement(5532..6296)
                     /locus_tag="IVZ90_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8263173.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IVZ90_002147"
     CDS             complement(6304..6981)
                     /locus_tag="IVZ90_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8263174.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IVZ90_002148"
     CDS             complement(7018..8403)
                     /locus_tag="IVZ90_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8263175.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IVZ90_002149"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVZ90_002149"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVZ90_002150"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVZ90_002150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8263176.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IVZ90_002151"
     CDS             9342..10199
                     /locus_tag="IVZ90_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8263177.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IVZ90_002152"
     CDS             complement(10280..10615)
                     /locus_tag="IVZ90_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263178.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IVZ90_002153"
     CDS             complement(10682..12052)
                     /locus_tag="IVZ90_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8263179.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IVZ90_002154"
     CDS             complement(12130..12666)
                     /locus_tag="IVZ90_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8263180.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3903 a         2617 c         2145 g         4301 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaagg
//
LOCUS       DACDBG010000075        12794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDBG010000075 DACDBG010000000
VERSION     DACDBG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.075"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..731
                     /locus_tag="IVZ90_002155"
     CDS             9..731
                     /locus_tag="IVZ90_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8263181.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="IVZ90_002156"
     CDS             789..2828
                     /locus_tag="IVZ90_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8263182.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="IVZ90_002157"
     CDS             complement(2937..4088)
                     /locus_tag="IVZ90_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8263183.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="IVZ90_002158"
     CDS             complement(4242..4772)
                     /locus_tag="IVZ90_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263184.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="IVZ90_002159"
     CDS             complement(4871..5221)
                     /locus_tag="IVZ90_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8263185.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="IVZ90_002160"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="IVZ90_002160"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8263186.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="IVZ90_002161"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="IVZ90_002161"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8263187.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="IVZ90_002162"
     CDS             complement(7624..8286)
                     /locus_tag="IVZ90_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8263188.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="IVZ90_002163"
     CDS             8470..10116
                     /locus_tag="IVZ90_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8263189.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..10235
                     /locus_tag="IVZ90_002164"
     CDS             10107..10235
                     /locus_tag="IVZ90_002164"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263190.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10202..11266
                     /gene="uxuA"
                     /locus_tag="IVZ90_002165"
     CDS             10202..11266
                     /gene="uxuA"
                     /locus_tag="IVZ90_002165"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8263191.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11374..>12794)
                     /locus_tag="IVZ90_002166"
     CDS             complement(11374..>12794)
                     /locus_tag="IVZ90_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8263192.1"
                     /translation="ESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYC
                     YYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYG
                     LSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKE
                     VLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLP
                     ICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLF
                     IPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDF
                     REQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIE
                     QIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISE
                     YIFELKEEALMDQSKKENQVTS"
BASE COUNT         4281 a         2358 c         2378 g         3777 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tcacaggaag aaaagattac cttagatgaa atcagacaga ttcctgcggt
    10261 caaagggatt gtaggcacat tgatggatat accagtagga gaggtatggc ccagggagag
    10321 gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta acactaaaag taattgaaag
    10381 cgtcaatgtg catgatgcca ttaaaattgg attaccttcc cgtgagcagt atatcgaaaa
    10441 ctataagcag acaatccgaa acttggcaga atatggaata gaagtcatct gctataattt
    10501 tatgcctgta tttgactggg tgaaatctga ccttgactac cgcttagaag acggctcatc
    10561 cacgttagct tttatatctg atgatattcc agatgatcca agagaaatcg tcgcgcgcat
    10621 cgaacattcc agcaatgaat ttgaattgcc gggatgggaa cctgagcgct tagctcatat
    10681 caaatccttg cttgaagcct atgcatcagt agacgaagaa aaacttcgag aaaattttgc
    10741 ctattttctc caatcaatca tcccaacatg tgaagaggta ggggtcaaaa tggctgtaca
    10801 tccagatgat ccgccatact cgatttttgg attacctcga atcgtaaaga atcgagaaga
    10861 tttagattgg ttgtgccgca tagtagacag cccttataac ggaatcactc tttgtacagg
    10921 gagtattgca gaagatccag aaaatgatgt atatgggatt ttagcagaat tttgtcaaag
    10981 agatcgaatc ccatttgcac atgttcgaaa tattaaattt ttagctaatc gagatttcta
    11041 tgaggcaccg cataaatccg aatacggttc attagacatg taccgcatac taaaaacaat
    11101 gtatgacaac ggatttaacg aatacatccg tcctgatcat ggaagaatga tctggaatga
    11161 gacaggaaga ccaggatacg gcttatatga tcgagcatta ggtgctgttt atctaaatgg
    11221 attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat aattagtgaa aaaaactgta
    11281 attttattat gtaaataaga aggatgttca aattcttgat acataaatcc ggaggttgtt
    11341 aataagcatt cggatttatg tatcttattt ggtctaggaa gtaacttgat tttctttttt
    11401 cgattgatcc atgagtgctt cttcttttaa ttcaaagata tattctgaga tcctcataat
    11461 cacttttttc cagtcttctg gtgtgatgat tccttttaaa atatagattt ttttgatatg
    11521 agaaggcaat tgtttcaaag ggcagtcgct gataacgatg ttgcagttct ccataaaatc
    11581 attttgtatt ttgatcgatt catttgaaaa aatttgttct atcttaacga tcagatcttt
    11641 actgatataa aaatcacgcg tatagttcac accaatcaca attcgcggct gttttttgta
    11701 gcgatcatac aaatgtacta ggtcttcaac catccattgc acattttcct tttctaaaat
    11761 aactggtaac aagtgttgat tttgttctct gaaatccata taaaattgtt caatggcggt
    11821 ttggacttct ttcaacacgc tatgttcttg ataaagcatg aaagtaggaa ggggcgtatt
    11881 tggttgattg aagttaagaa atccattata aagtttattt cgtgcgaatg ccaatgagta
    11941 aagaatcttt tctttttcag gtataaacaa atcaaataca gtacaaaagg actccaccat
    12001 ttctcttgaa taaagaacat gaggaacgtt tgcttttttt aaaagtttgt attgctccaa
    12061 gcttgtttct tccgattcga ttttcgtgat agaaagtcga gctaaaatat tcactaagat
    12121 gcgttgttcg cgacaaattg gcagattagg tcgcaaaata tctacagagg gatcaataaa
    12181 aaagtcaata tattcatact ctgtgaattc tttttgtacg tctttcacag aagttgcagg
    12241 aaaattctct ttacatattt ttaatagaaa atataattta tctatcatac cgctatttag
    12301 tcggtcaagt acttcttttt taaaaaaagt attcatcata aaagccacta cttgcggatc
    12361 ttcatcataa aattctgtag cagtacttga aaagatgttc caataaatat ctaatgctgc
    12421 atattgaatt tgtacctctt ttccagaaaa agactttctg ccattattag aaaaggagat
    12481 agacaaaccg tacaatgcta aaaatgcatt aatctttttc attttcgtat acaaataaga
    12541 ggcactatag tttgtttgct gacttaaact gataatcgaa tttgtctgac agctgaacaa
    12601 agaagtcaag atctgataac tgactgattc gttgcagtat cttccaaata gacgataata
    12661 gcagtaagcc ggatcaattc cttcaaaatt caactttaaa aaaggttttt gcttatcaat
    12721 atgcagttct gttagattga gacgctcaaa gtcttgttct aattctttga tgtatagtaa
    12781 aaaatttgat tcag
//
LOCUS       DACDBG010000076        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDBG010000076 DACDBG010000000
VERSION     DACDBG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.076"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVZ90_002167"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVZ90_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8263193.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVZ90_002168"
     CDS             complement(322..891)
                     /locus_tag="IVZ90_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8263194.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVZ90_002169"
     CDS             1077..1979
                     /locus_tag="IVZ90_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8263195.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVZ90_002170"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVZ90_002170"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8263196.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVZ90_002171"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVZ90_002171"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8263197.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVZ90_002172"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVZ90_002172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8263198.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVZ90_002173"
     CDS             8547..8732
                     /locus_tag="IVZ90_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8263199.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVZ90_002174"
     CDS             complement(8794..9990)
                     /locus_tag="IVZ90_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8263200.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVZ90_002175"
     CDS             complement(10160..11596)
                     /locus_tag="IVZ90_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8263201.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVZ90_002176"
     CDS             11889..12698
                     /locus_tag="IVZ90_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8263202.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDBG010000077        12504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDBG010000077 DACDBG010000000
VERSION     DACDBG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.077"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..852
                     /locus_tag="IVZ90_002177"
     CDS             <1..852
                     /locus_tag="IVZ90_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8263203.1"
                     /translation="KKNIKLIILRIGMLLCLGVGLYPVITQAYTYYQTQQTLNAYKEK
                     YDEASEKENEEIFKKYSEINRKRKETEQTLVTIPEDNNNIPSNGNLQKDKLGELVGEI
                     LIPKINVDLPIYNGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFTDL
                     ELLKLGDKFFINVAGKKLAYKVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGENSH
                     RLLVRGVRIPYKKNIVEKEKKKEDLKVKAQINWYPIIVVGVLSIIALIVIIKKIVAKR
                     RGMKNEE"
     gene            842..1921
                     /locus_tag="IVZ90_002178"
     CDS             842..1921
                     /locus_tag="IVZ90_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8263204.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNNQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSEAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            2260..3060
                     /locus_tag="IVZ90_002179"
     CDS             2260..3060
                     /locus_tag="IVZ90_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263205.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            3148..3954
                     /locus_tag="IVZ90_002180"
     CDS             3148..3954
                     /locus_tag="IVZ90_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263206.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            4186..5055
                     /locus_tag="IVZ90_002181"
     CDS             4186..5055
                     /locus_tag="IVZ90_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8263207.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            5075..5422
                     /locus_tag="IVZ90_002182"
     CDS             5075..5422
                     /locus_tag="IVZ90_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263208.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            5435..6697
                     /locus_tag="IVZ90_002183"
     CDS             5435..6697
                     /locus_tag="IVZ90_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263209.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            6777..8153
                     /locus_tag="IVZ90_002184"
     CDS             6777..8153
                     /locus_tag="IVZ90_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8263210.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEGYDFFEYLVILMHERYKLMEKAGLQHITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKHVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQSEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            8150..8779
                     /locus_tag="IVZ90_002185"
     CDS             8150..8779
                     /locus_tag="IVZ90_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263211.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            8798..9277
                     /locus_tag="IVZ90_002186"
     CDS             8798..9277
                     /locus_tag="IVZ90_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263212.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            9779..9832
                     /locus_tag="IVZ90_002187"
                     /pseudo
     CDS             9779..9832
                     /locus_tag="IVZ90_002187"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9836..10435
                     /locus_tag="IVZ90_002188"
     CDS             9836..10435
                     /locus_tag="IVZ90_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8263213.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            10451..10927
                     /locus_tag="IVZ90_002189"
     CDS             10451..10927
                     /locus_tag="IVZ90_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263214.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            10944..11846
                     /locus_tag="IVZ90_002190"
     CDS             10944..11846
                     /locus_tag="IVZ90_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263215.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKKDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFTVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            11919..12113
                     /locus_tag="IVZ90_002191"
     CDS             11919..12113
                     /locus_tag="IVZ90_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263216.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            12218..>12504
                     /locus_tag="IVZ90_002192"
     CDS             12218..>12504
                     /locus_tag="IVZ90_002192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263217.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKL"
BASE COUNT         4794 a         1717 c         2469 g         3524 t
ORIGIN      
        1 aaaaagaata tcaagctaat catcttaaga attgggatgt tgctatgtct aggagtaggt
       61 ctataccctg taattactca ggcttatacc tattaccaga ctcaacaaac gttaaatgcc
      121 tataaggaaa aatatgatga agcttctgaa aaggagaatg aagaaatctt caaaaaatat
      181 tcggaaatca ataggaaacg aaaagaaaca gaacaaacct tagttacgat tcctgaagat
      241 aataacaata ttccgtcaaa tggaaatctt caaaaggata aattgggtga attagtaggt
      301 gagattctta taccaaaaat aaatgtggat ttaccgattt ataatggcgt aaacgatttt
      361 caattatcag caggagcagg aatacttact ggaactgatt atccgaccgg tgggattgga
      421 aaccattctg tcatatcagc tcattcaggt attcctaata aaaaatattt tacagattta
      481 gaattgctca aacttggtga taaattcttt atcaatgttg caggaaaaaa acttgcttat
      541 aaagtagatc ggataaaaat aattctacct accgaatttg aggatttgct tcctgtagca
      601 gggaaagatt atgtaacgct tatgacgtgt actcctgttg gagaaaattc tcataggtta
      661 cttgttagag gagttagaat accctacaaa aaaaatattg ttgagaaaga aaaaaagaaa
      721 gaagacttaa aagtaaaggc gcagataaac tggtatccca tcatagttgt aggtgtttta
      781 tcaataatag ctttaatagt cattattaaa aaaatagtgg caaagagaag agggatgaaa
      841 aatgaagaat aaaatactac tttttttgac tcttatttgc actttggttt taacaattta
      901 tcctgcaaat cagctctatc atcaatttaa aacgaaagag aatgtggcgg aaatccaaga
      961 gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg gcaaaaaaag tcgaacaaat
     1021 aaaatcgaac agcggtaacc aaggattaga caataatcag aaaacagacg gcggatcatt
     1081 tgatattggt gaagctgaag gaaaggtcaa tccttacaca ggagatgttt tttcaaaaaa
     1141 tcaaacgaaa gattttgaac catctaccta ttcagaggcg gttggttatg tctatattcc
     1201 aaaaataaat gtgctagagc ttttgtttct aggcgcttca caggagcatt tatatagagg
     1261 tgtggcacag atgagtgcta ctagtctacc tttatcaggt aaaggtcatc aaacagtaat
     1321 agcaggtcat cgtggcggtt atacgtcacc acaatttctt tatgttgatc aattagtagc
     1381 cggagaccac atttatatag attatcttgg tgaaaaagca gtttatacgg ttttccgaac
     1441 agacgttatt agaaattacg gtcagaattc aacactgact gatattcctg atttagatat
     1501 gttaacactt tatacttgtc atccatatcc tcagaactac gatcgtttat tagttagaag
     1561 tctaagagat gaaaaagcaa aatacatcac gccacaagta acagttggca ataccgcata
     1621 cgctgatgcc attgatagcg agtccgtttc agataagaca gaaactgtta catcttctaa
     1681 tgatttattt gaggatgttg atcgtgccaa caaggaatta gatggaaata atgatcttaa
     1741 tattccggaa caaggtagcg tagagatgca agacagaacg tttgctgaaa aggcaagttc
     1801 aatatttaca ggaattacca ataaacatca gaaaatgctt ttagtcattt ggactgtaat
     1861 aggagcatta gcaagtctaa aggttcttat tcttttaatc aaagagtttc gaaaagcata
     1921 atgaagatta taaaaattga tagagtacag taaaaaggga ttgatactat tattgatctc
     1981 ttttttattt tgaatagagc tagattcact tttaaataaa atcaaaactg atcttaattc
     2041 taaagaaaaa gtcaagaaaa aaatacaaca aattttttta tcagaatatt tatcagaata
     2101 ttattagaaa agataaactc aaaagcttaa tttaataggg ttattggttg tatttagaaa
     2161 taatgtatgg aaaagctata aatcaataga taagatgtag aaaatcttat ttgtgaatga
     2221 aaaacaagtc atcgtaaaat attaatagga ggtgtagtaa tggtgggaat atcaaaaaca
     2281 gctttagact cattagctaa actatttagt agcaggtttg atggtgcaga atttgttgaa
     2341 tatctaagcc aaaacgaaag tggaaaaact ctgatcgaaa caatgaaaaa agactatttg
     2401 aatgaaaatg actttccgac atggacgaat attgaagatg ctacatggga tgtagtatgt
     2461 acgatatatt ctccttgggt aacgattgct gattttgttt tagataaaat tggaggggat
     2521 acataccctg atgccaaatc tcttgctaca agtgacttta aaacgttcta tggttatcta
     2581 ataggtctaa taggcaatgg aatagatttg aaagttacgc caaaacaaac acctgaagaa
     2641 attgaaaaag aaaaaggaga ggttcaaatg gcttatgcaa atgacagaga aaaagtaaaa
     2701 ttattggaag gtaaattatc tgaagaagaa cttggaaaaa tggtgcaaga aatcaatgaa
     2761 gacagcaact attttcctga attgaaggta tatcaaaatg acgaagattt ctttaatgaa
     2821 atgtttagta atagtccatg ggaagcagtg agtaaaacaa atttaggaga atggaacttt
     2881 caagatgatc tagttgcatt tagcaatgat gggtatttac gatcatattc agaaagtgag
     2941 tatagagctg aattaaaaac gtatgaaacg gatattctga tgagctattt aaatctatta
     3001 gagagtggtc agggagagtt aagagataaa gttgaagaac tattaaaaga agagctatag
     3061 ggattatttc aacataaaca caagcagaaa actactaaac aaaacgtacc atgcaaaaag
     3121 tcggttaaac cggctttttg ctctattatg agaggagatc aaaccatgag tgcaacaacc
     3181 gatatggctt tagaagcagt aataaaagat aatacaggaa atgtcgaaaa cgtagaagac
     3241 tttagcgata ttgcaatcaa tctttcagca tggatttcag ctgtaggaga agaaaatatc
     3301 gaatttgaga tcaagaatat ggatgactta aaagcaaaat ttccttttat cgcatcgaaa
     3361 ctaagtcaag ttgccgagga taaatcgtgg aatgaattag ttagtttttc cgaaaaaatt
     3421 tatgatatta cgcaagaaga tgagtgtgtt tcatgggaaa tgaataatca atacaagaaa
     3481 attgacgctt tcgtcaaatc acgtatagaa ggaacagctt ttgagtttga aggtatagat
     3541 accgcagata attggttaaa taaagaatcc tttattaaaa gtatagcgga tgatcttgaa
     3601 aaacaatggt ctaaggaaga ctatgcagag tacctaaaaa cagaaagaga tacagaggtg
     3661 aaagaaaaac tcactgaggt atgtttggat gaacatattg atcccaatat cgtaaaacaa
     3721 gaagactatg aagtggttat tgataaagtt cctactgatt ttgaggaagt tgtaaagtat
     3781 ctagttgaag aagcattatc gcaatactct tcagatgagg aagtggacga agaaactgtt
     3841 cgtaccagag tttggcaact ggtttttgat gacaaagatt ataaatattt gagtgatcaa
     3901 aactcggtta attggagtgt acgattatta attgatcaaa atgatattga ataaaataga
     3961 gctgaaaagc tctatttttt ttgttaaaaa aactagctat tttattaaaa ataatttggt
     4021 tcaaatttta tttttttaac ttgataattt cttgccatcc ttttttaaat tggctatata
     4081 tcaaggttta tagtgggaat aaataaaaat acatttgaca aaatttacaa tatagtttat
     4141 tatatattta tcttattatg ataacacaat aaaaggaatg gtggtatgaa aaagaagaag
     4201 tttagtcgat cgttgttagg aattttggtt ttagttgcgg ttggtcttag tggcaagggt
     4261 tactttcaga ataacagcac tcaggaacat gattttcaga tcaaaagtag tgttcagata
     4321 ggtaataaag cagacagtga atcaaaagaa tttttaggga tacgttatga tccaaacaaa
     4381 tatccaaata atatcgtaaa cattaatgat gggaaatata cactgacaaa agaagaccat
     4441 gaagcattat cagaaaaagg cagtcaaacc ttttgggtaa attatgcaga tttagatcaa
     4501 cttggaagag caggaaatgt tgaagcgttg atcacctatg atgctgtaaa ttctcactca
     4561 tctaaaaatg tggaacgtcc atctttcaaa agttctgtcc atattgcagg agaatacaag
     4621 gatggagtat ttgatcaaga gagagatact tgggtcggca aagattcaaa caatgaaatc
     4681 actcagctag aaggctataa aggttatctc tataacaaaa gtcattcatt agcatggtcg
     4741 ctaggaggag atatggaggt acacaattta acacttggaa cgagagcgca aaatgttggg
     4801 aaaaatgatc agaatggagg gatggcttac tcagaaacga ttgtacgtga tgcaatctat
     4861 aaaaataaga atgaaaaagt attgtataaa gttgatccaa tttataaagg gaatgaatta
     4921 gttcctcgtg gttctcatgt cgaagctttc tcagttaacg ataatggaaa aacggtaaac
     4981 cttaatgttt atgtatttaa caatcaaaaa ggaatcaaca tcaactatca aaatggtact
     5041 tgggtaagag attagaaatt gagaaggtga agaattggaa acaataagca tcaatggatt
     5101 agataaatca gttgcaaatc gaataaaaaa aggaaatggc tactatcttc tgaagaaggg
     5161 taaatattca gttgctagag agttagaaac aatgagtatc gtgtatgtag ctaagaatca
     5221 ttttgacgta gaacaggcta tttttgatcc agattctgaa atagggaaat gtgttgaatt
     5281 atcagagaaa actgttagta caaaaggggc gatggtcatt gagagagaaa tgttagacga
     5341 aatcagagta atcattccta aagatacgca gatgtgtgag gacagcataa gtgacgatga
     5401 accaaaatca gaaagaaaat aaggagatat tcagatgagc gaaaaatcca ttgaattaaa
     5461 ggaatataag cgaagcgatg gagaaaaata ttatcctgta gccagttttt ctaaatcagt
     5521 atatctggtt ggacgtgcat tggtagttgg tgcaattggc atatatattt tgcattgggt
     5581 aacaaaggtt gtacaactgc ttgtagatac cttagtaagt attcttgtct cgttaaatga
     5641 tgttaacgga acagatgatt taggattaaa agagatgttt aaagagttac tcaatgcgtt
     5701 ttttcccaat tatttaagtt attcatataa tgtacaaagc ttttttgcaa agctacactt
     5761 ggtagacatc tctattttag tattagaagc aggaacggta ttctttgttg gatatttggt
     5821 tactttgatg ttcaggcatc actatggaga acaaagacca ttctttgatg atatggaaag
     5881 tagaaggttg aaaagagaag ctctagaagc tatggatgtg aagtatgatc caaacaagat
     5941 tataggagat gaaacgaaca acaaaaaaag aaaacgaaac aaaacgcaat tagaatccga
     6001 tgtgctatca acgcttaaaa atatcaaaat ttctgttcat acaagacgtg atctaagtgt
     6061 caatgaagat attcgagaat atatcgtaaa aataccgcaa ccaaaaggga agaaagttag
     6121 agaaacagtt ttacgacaaa ttcaagattt agggtcgatc ctgacaagag caacaagagg
     6181 gcgagctaaa tttggggaaa aagaagacga agtttcaggt gattacttca tcttcagagc
     6241 atcagtaaac attgaaagag aacgtaagga aagagtaagt accaaaaaca aggaaacgga
     6301 aaaatcaaca gcatccattg cagtttcaaa ttcacaattt gcttttccaa tcaatctctt
     6361 tgcagataac tccaaaaata tccaaaacca aaccgaaaaa gctagaaaat atgccgaaac
     6421 tcaaaagcaa tctttagaac tttatctatc ttctgctgac gtacctgcaa gtctaacaga
     6481 tatgcacgtt gggaatacat ctattgaata tttgtttact ttatctcgta ccagaaaaat
     6541 tcaactaagc tcaatgaaag aagaaattga aacttacttg catatatcag gtgtaatcgt
     6601 gcggatcaac gcagggcgta ttcaaattac tctaccatta ggagaaaatg atcgtattcc
     6661 aattgatgta ccatcaatga tcaatagcgt gttttaaaag acatcacaaa tagaaacaga
     6721 aaatacaaac taccagttca caaacagaaa agtaccgaga aaagtgagga atagaaatgg
     6781 ctatgaaatt attagagaaa gtcaaaaaga gagccgaaaa agctgaatct attttgctag
     6841 ataaagaacc taatataaag cttaatggaa aattcagtgt caatatgttg aacacaaagg
     6901 ctaaagaccc gacagatgct atttttggaa ttaagcctga tggcacaatt gctcattttt
     6961 gcatcagcga aatgccacac atgttggttg caggaacgac aggatcaggg aaatcagtat
     7021 tactgaatga aattcttgtt acagcaatgt gtcactcaac gcctgatgaa ttaaaaatag
     7081 gaattgtaga cccaaaaagg gttgaatttg gaagatacaa aaaattgccg ttcatgttgg
     7141 ctgatccaat tacagatatg gatgaaggtt atgatttttt tgaatatctt gtgatcttga
     7201 tgcatgaacg ttacaaactg atggaaaaag cagggttaca acacatcacg ctatataacg
     7261 aatatgccga aaaaaatgga ttagatagat ttccctatgt gatcttatta gttgatgaat
     7321 attcacagtt ggttggaaca cataaagaag tagaaggttt gattgttcaa ttaggacaaa
     7381 tggcaagggc ggcaggtatt catgttatcc tcgctacaca atctcctcga tcaacagttg
     7441 tcactggtat tatcaaagcc aattttccgt cacgtgtttg ccttatggta gcaagtgatt
     7501 tagaaagtag gatcgtatta gatgaaggcg gtggagaaag cctaagtcca aagggagata
     7561 tgattattaa gcacgttaat ggaagtatgg ttagagcgca gggagcttac atcagtaaca
     7621 aagaaattga aacaattttc aatcatctta gaaatactat gccagaacca gaatatgttg
     7681 attaccgagc aatcgtagct gaatctcgtg gtgagggtgg agaagacgag gattttggtg
     7741 gaattggtaa agctcctgta agaaaatctg ctaaaagaaa tttgaacaca cctttagcaa
     7801 attcatcatc taaagagcca tcaattgaaa agaagagtac gtttgctcta aaacttgaaa
     7861 aaaacaaaga agaaaaagaa aaacaaggag ctaaaacact agaaaaactt caatccgaaa
     7921 aattagaaaa agttttaaaa gcattagata aagctgattc tgaaccgcaa acctcaaaca
     7981 acacgaataa tgaacagccg atttcatcta aaactaaacc gaaaactact gatccgaaag
     8041 tttcagtaaa cgtgaccatc acaaccgata agtctgagaa gaaacaagta aacggaacga
     8101 cttcacctat catgcaaaaa atgttgaaag ccaaaaaaca ggtgcataaa tgatcattgt
     8161 cagttctttt ttgaggtggc ttacgccata taaatataca gatgaagtag ttgtgaaacg
     8221 aacgaagaaa agagataaaa aagcggtact aactcctaaa cagatgcttg atcatggcat
     8281 tttaggggtg aaggttttag aagtgaataa accgtttgaa tcaaatagtg caagagaaaa
     8341 gttgcttgga gctgaagccg atgtcgcata tttttaccat gacaaaagca agcatcaaat
     8401 agctattaaa ttcaatccaa aatcggcatg gcggtatcaa gcaagagatg acttgaaacg
     8461 taggaaactt agaccaatgc tctatccaga aggaaaagaa gcatttgata gaacccatat
     8521 cgttcccatt ggttttcatg gtacagaatc agataaccgc ctattagtag gttggaacag
     8581 caatcaaaat agagttcaga tcaataaatt tgagaagaaa gtaaggaaat tcaacgatcg
     8641 aaaaacgatc ctatggtttg cagatatttg taaacagccg gatggaacag caaagtggga
     8701 agcggtgata tttgacgaaa atggtaaaaa agtaatgtct gaaacatggc atgataaagc
     8761 taagtttgtt tggcagtagc agtagaggtg aaaaagattg ctagaacttt ttgaagcaaa
     8821 tagaacaggc tttaatcaaa aattagaagt gggagaaact atagaatata aagataaaaa
     8881 ctatattatt atcggaatct ataacacgag aattaacaga ataggtgatc cacgtataac
     8941 cagtgacttg gtttgtcaat ctgttaacta ttcacctgat ctgacaagca ggtataagaa
     9001 atacgtgtta ttagaatata gacacaagat tactgatgaa attggtgtaa acaacacacg
     9061 aaacatattt aagatcggaa cagtgattcc ttatagtaac aacgaagaaa aaatatttta
     9121 tcagatttac ggaatagaaa agtttgaata tgaacatgtc gatctgctag tgacgtacca
     9181 aatgcgttta atagaacctt ggtcacaggc agaaattgat aaagcagtaa aattgaaccg
     9241 attatcaaaa tttaacgtat taggaaatgc cttttaaaaa tcgcaataca caaacagaaa
     9301 aataccaaaa aattagcaat gaacgtaggt aagaacatga caataaataa taataaccct
     9361 aaaaaatata atgctcttgt ccaatgctat atcgaaaaca aaattacaat gcaagaagtc
     9421 gctgatgaat taggcaaatc aaagagccta gtatcacacg attttaagca caatgttttt
     9481 ccgaacccga ttgagctgag gaaaaaattt caaaagagag tttatcaaga aataggtaag
     9541 tggattgatg aagggaatgc aattgaccct ttaattgaaa aatatccttt tttaaaaagc
     9601 aaaaataaaa ttcaaaggtt aattcgtcaa ggcaatattc aagctaaaga acctgttttg
     9661 acaaccaatg atttggctaa aataaatctt cataaacaaa ttattgaaga ctttgaaaaa
     9721 aatctttcaa caactgaaat tttaaaaaaa tacaatattc cgagaggaaa gctaacatcg
     9781 actcttttta gagaatatgg agaatcggca gttactaaag caaaggaagt agcgaatgag
     9841 agaaaaaaac aaaaatagta gtcgatacct agtagcgact gtagtttttg tttgtgtatt
     9901 gattttcctg tttgattttt tgcagattgg aaaaagcata attggaaata cattgagtgc
     9961 aaaggaaatg gttgctttag gtggtttgta tgaaggacac ttgttcccga atatattcat
    10021 ttctatcgtt tctcatatta acttgtacca tttaatttct aatttattgt tcttagtagt
    10081 tcttgaagaa gcactgaatg atcagtatga gtggttaaca ttattgatcg ttttaatcat
    10141 atcaggattt ctaggaagct tagttccttt tctgctacac agcaacatcg taagtatggg
    10201 gcttagtgga ggtatttttg gatggattgc tttatttgcg attgcataca tccgttttga
    10261 tagcaagaaa catttattac taagtgtttt tgtattattg ataatcggca ttttactggt
    10321 atctactttt gaatcaggcg agacaaacgt cttgtcacat gtaatcggat ttgtctgtgg
    10381 gatgatcttg ggatttattc taaagaacgt ttcattgatc aaacaaaaga attaaaaggg
    10441 agtgctaaaa atgccaaaaa aaaaagaaaa gaaaattcca gtgtttttgt ctgtctcaga
    10501 aatgttatca cttcaaatac tgttaagaaa aaagattgaa acaatagacg aagaagttag
    10561 aaataaaagg gctatgtacg aacaacgtga agcgctaaga gaacgaatcc actattttga
    10621 tgatatacac actaaaatct atgaagcacg tacaaatcac gaagacgaaa gcaaattagg
    10681 attagctcaa caacgaatat ttgaatggct gaatacgaat actgagcaag aaccgttaag
    10741 agcaattagt aatctttttg aatttggaac gaagcattgg tttaacgctg tagaaaagga
    10801 tgctgactta caagaagcgt tcgatacact aagcgaggat agcaaatcca tgcgagactt
    10861 attgatcact tatctaaatg agcatgaatt tgaacagacg aaagttaaaa agaaaaataa
    10921 aaaatagaaa gttgtgatgt atcatgctgt ttaccttatt aggttattta tgtttagttc
    10981 ttgctttttt tggagcaatt gctatatttg tcggtcagtt tcaagcaaat aatccgataa
    11041 tgcgtaacat aggcattgga attgttatca tatccttgat tgtatttggt gccacaaaga
    11101 tttttgggag taatgatcag cagtttggta tttctggagt tgataatagt tatgctccag
    11161 attcattggg aagagtagtg atagatggaa cgtttgagaa tgtaacgagc tacaaaaatg
    11221 tttcattgac agtgaaaaaa gatgacaaga aagcatcaga tgacgttctc gctacgatca
    11281 agccgtctca atcacagttt gaagtgtttt atacagtcaa agagaataac cctgtacgca
    11341 agctaacatt agtttttacc gttgacggag aagaatacta taaagatgta aaaatcaata
    11401 aagcatcaac gaaaaagatt gtcgaatcag cgaaaaaaga agtagctaca gaagaaagta
    11461 caactgaatc aagttctact gtcgaagttg aactaccaga taaaattgat tttgatcctg
    11521 aaaaagtcaa atcgtttgaa tctgtttctg gttctatggc agatcggttt aacactgaag
    11581 ttttcaaagc tatcaaaact gttaaattgg atactgaaaa gcaaaggatt aatgttacct
    11641 tattcaatgc agttgaatct atggatcagg ataaaaaagt tacggttgca agagaactag
    11701 ggaaaagagt aattgggttg tatcgaggta cggcaaaacc agattcagac ggttcagatt
    11761 tgccagttca cgtagtttat gaagacggta aagtttttgg agactctaca gcaaatgaag
    11821 cactaacgat taaattagct aaatagccgg aaaattataa taaacaagtg gtgtcacacc
    11881 accatcgcat aggtgtgaca ccttggagat gattactgat ggtagtaaat aaagaaaaag
    11941 taagaaagta tgttacttta aaaatagctt ctgttgaata tttagaaaag cgagctaaag
    12001 aagaagaaaa aacaagaggt aagaaggtaa cagtttcaga gttgttagaa gaattaattg
    12061 agagtgacca aacaataaga cgtgcattta ccaataggtc tgttaaaagt tagggtgata
    12121 ctatctttaa tttcaaccaa atttctaata tatgatgaat agtttccttg tacaaaatat
    12181 ctaattaaga gataaaagtt aggaggattc attcaaaatg attatccaaa agtttgactg
    12241 gaaaatgaaa ttaggaatta gtcttgtaat attgggattt attatttttt tggggataaa
    12301 agcacaaaaa aatcaatcta cttttgaaac tatgtcttct caaattattg aggaatgttc
    12361 caaaatttat tattgtgaga acaaaactga agaatctaat ggcttttatt atatcgatca
    12421 accacatgat gttgttataa aaatggagtc acatgaagta aacgaatgga ttcaaagaag
    12481 tactaatgta tcaaaaaagt taga
//
LOCUS       DACDBG010000078        12307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDBG010000078 DACDBG010000000
VERSION     DACDBG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.078"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IVZ90_002193"
     CDS             287..694
                     /locus_tag="IVZ90_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263218.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVZ90_002194"
     CDS             708..1109
                     /locus_tag="IVZ90_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263219.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVZ90_002195"
     CDS             1111..1482
                     /locus_tag="IVZ90_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263220.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="IVZ90_002196"
     CDS             complement(1518..1817)
                     /locus_tag="IVZ90_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263221.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="IVZ90_002197"
     CDS             complement(1823..2053)
                     /locus_tag="IVZ90_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263222.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2670..3332
                     /locus_tag="IVZ90_002198"
     CDS             2670..3332
                     /locus_tag="IVZ90_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263223.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3411..6636
                     /gene="fss3"
                     /locus_tag="IVZ90_002199"
                     /pseudo
     CDS             3411..6636
                     /gene="fss3"
                     /locus_tag="IVZ90_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6750..7064
                     /locus_tag="IVZ90_002200"
     CDS             6750..7064
                     /locus_tag="IVZ90_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8263224.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7077..7451
                     /locus_tag="IVZ90_002201"
     CDS             7077..7451
                     /locus_tag="IVZ90_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8263225.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7461..7805
                     /locus_tag="IVZ90_002202"
     CDS             7461..7805
                     /locus_tag="IVZ90_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8263226.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7876..9228
                     /locus_tag="IVZ90_002203"
     CDS             7876..9228
                     /locus_tag="IVZ90_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8263227.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9288..10430
                     /locus_tag="IVZ90_002204"
     CDS             9288..10430
                     /locus_tag="IVZ90_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8263228.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10455..10709
                     /locus_tag="IVZ90_002205"
     CDS             10455..10709
                     /locus_tag="IVZ90_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263229.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10965..12149
                     /locus_tag="IVZ90_002206"
     CDS             10965..12149
                     /locus_tag="IVZ90_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8263230.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12164..12283
                     /locus_tag="IVZ90_002207"
     CDS             12164..12283
                     /locus_tag="IVZ90_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8263231.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         4168 a         2017 c         2646 g         3476 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgtaggat cgaatttaaa ccataattgt
     1801 gtctcattaa aaagcataac cattactccg atctgttttt aaaattagtt tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
     1921 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat
     1981 cttttttgct gctttgctca taaaagtctg catattcatc actgatattt tttctaataa
     2041 agtcttccat cataaaaatc acctcaaaac gattatacga acttacgttc tcgttgtaaa
     2101 gcgatatttg aggggcaaaa agggggcaaa aatacgttta tctttgatat atctcagatt
     2161 gaacatagtg tttattatat gtcggtattg ttgatttata gctgttttga gggtattaga
     2221 tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct gctcgtatca
     2281 aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc
     2341 ttgtatatca agggcttttt cttttttctc tatcacagtt ttaagccatt tttggctgtt
     2401 ttggaccatt tttcaatcat attcccttaa aaattgtcct agattgtccg acattgacta
     2461 tcagtgtcgt caaaataggg caaaatttag tagtcagctt gacgacaaat ataatacgaa
     2521 aaataccaaa acatttaccg aatttagcaa cttttgaacc caagtttctc aaagggtcag
     2581 ctattagcaa aaaagacaaa acatgtactt ttctactaaa aagttgatgt gataggcttc
     2641 tatctaaata actattagga gtttttatcg tgaagcaaaa tgatggacgt atcatttgga
     2701 aaaatcaaag tgagttaaaa ctgattttaa caattaatga attcatcgaa aaacacggaa
     2761 ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa tccaaattct gcaccaagta
     2821 tgtggttcat taacaaaaaa tatggatcat gggagaattt attgattagt ataggacgag
     2881 aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca agaattattg gaaatagtag
     2941 aagcctttat taagtgcgag aagataacct ctcaacgaat gtacgaacaa aaatcagtag
     3001 ggaagaatat tccttcactt agtaccatta aaaagatgtt aggagatata cgtccacttt
     3061 ttaaagaaaa aaatgatgga tctcgattta ctgatttcga actgctgtta gaactaaaaa
     3121 atgaaataat ccgattaaaa ctacaggatg atttatcaat gacaaagttt cgaaaactag
     3181 ttcaatcccc aaaactacca tcagttgata caattatgaa aagaacgaac aaaaattggg
     3241 aggaattgat ggcagagatt ggatttgatt atcgaagaat caaaatttat aagcagagaa
     3301 acaacctatc caagacaaag aaaactaaat agtatcactc taaatcagac gttcaaaatt
     3361 aaaatttgga cgtctttttt attacccaaa aacgaaagga gatttatttt atgaaaaagc
     3421 caagatttaa gaattggcgt ttgctcgcaa cactgagtct attatgccaa acaattggtg
     3481 gatcgcttgg cccaacgatt gcctttgctg atgaaattac tcatccacaa acggtaactg
     3541 tggaattgga tttggctcac cagtatgctg tcgaaggtac atttagcgat ggtcgtccca
     3601 tgtcagaagt taccgttccg cactatgcag tttacaatgg tgtgaagcaa gatattttct
     3661 gtattgagcc aggggtgccg atttacaatg agttcactcc tggttatgag aaaaatccat
     3721 tgcctgatat gtcggagaaa gcgaaattag tttccgtttt atggaaggaa gctggtacgg
     3781 atgtggatac acatattgtg gcccagaaga tgatttggca agaagttaat ggctacgctt
     3841 cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga gtcaaaaatc
     3901 aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc
     3961 gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc ggagtttgat
     4021 gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca actagtgatt
     4081 actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc agctaataca
     4141 ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg ggcaattgat
     4201 aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt gaagattaaa
     4261 aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt agactttgga
     4321 aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac attggatgag
     4381 attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc ttatacgatt
     4441 gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt gacttcaaaa
     4501 aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac aggaactgat
     4561 ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa agacagtcca
     4621 accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa
     4681 gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa agctagtaat
     4741 ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg
     4801 gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga aaccactttg
     4861 acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt taaaggagct
     4921 gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga ggcttttaaa
     4981 ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac tttagatgaa
     5041 aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca agaaaccaaa
     5101 gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa aaaggtggat
     5161 gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca agtcattcgc
     5221 tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga aactggcttc
     5281 aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac aggtgccgaa
     5341 gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg caagtttgaa
     5401 aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg atttcaaaaa
     5461 attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta cactaagagt
     5521 gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt
     5581 ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt gatgctctat
     5641 gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga cggaggcaaa
     5701 gaattgacag accttgattc taccgagcta gttgataaat tgagctataa cttgcatgaa
     5761 atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc
     5821 caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc aacgttggcg
     5881 aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga acaagttttg
     5941 gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta tgaagcaggt
     6001 gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt caattgtacg
     6061 gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc gcaaaccttt
     6121 actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga tgtactggat
     6181 ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa tacgaccaaa
     6241 gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt cactaaaacc
     6301 gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt taccttcaaa
     6361 gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga tctaaaagaa
     6421 aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc agaacaaccg
     6481 gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa gacattccca
     6541 caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt gatctttgcg
     6601 actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa acaactaaat
     6661 aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc
     6721 aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg ccagatatgg
     6781 ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact gaaggatatg
     6841 ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag caacgagccg
     6901 atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat caagatcaac
     6961 cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt
     7021 ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa aagaagatga
     7081 gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta cgattttccg
     7141 cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac gaggtaaaag
     7201 aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt agtatcccag
     7261 caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc aatccaatca
     7321 tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg ttcattaagg
     7381 ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac caaaatgaag
     7441 gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat tgtctcgatc
     7501 aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc tatgatgaaa
     7561 aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgcc gaagaagatt
     7621 ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc gaagtaaatg
     7681 aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa atgccggatg
     7741 ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata gcagtaaatg
     7801 actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt tcgattggag
     7861 gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca gcacttggtt
     7921 tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat
     7981 tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat agaaatcgtt
     8041 cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg aagcacgatg
     8101 ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg acaaaagcta
     8161 gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag tgaaggattt
     8221 ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc caaaatctat
     8281 taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg aaaatatcaa
     8341 gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga actagtagaa
     8401 aaggaactca aagactccta tgtggagtac actttactgt atgatataat tgccaatcga
     8461 attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa aaatcaagta
     8521 tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg tgggaagact
     8581 tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct ttttgttctt
     8641 gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt ctattcccaa
     8701 aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac tcgtagcaag
     8761 gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct tggacttcca
     8821 ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac gacgaaggaa
     8881 agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca atctggtttc
     8941 tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg gattcgcgat
     9001 caatttaatt tccgggtggc tttgggtcgt atgagtgaac tgggatattc catgatgttt
     9061 ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta tgtagatacg
     9121 ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata tgatttctta
     9181 agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa taatagtatg
     9241 tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg acatttttag
     9301 atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt caccagtgta
     9361 tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc catgatacaa
     9421 gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt caatctctcg
     9481 gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt gcgggaaagc
     9541 ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccgcta
     9601 ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat cacgaaggag
     9661 gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat gtggaatggc
     9721 aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc cttatcggac
     9781 attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct ccaacatctg
     9841 atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg gggggacaag
     9901 tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc aaaatcgcat
     9961 tgccggtaga tgatggaatt tctgtagcgg gtatgttact gggaaatttt gaacaaggca
    10021 atcgggttta tgagataact ggaacagcgc ctactttgtc aacgaaacaa ggcggaacga
    10081 agatcatgct tcggaaaaac gaaacagaat atcaagaggt gaagccaggg aacagtgtga
    10141 acctggcttt tcctaattcc acaagtcgtc gaggccgtct gggaaaacaa agtgtgcata
    10201 ccttattaac aggagaccaa caagcagtcg tcacggatca gtatcaaatt cgaaagttaa
    10261 cacctaggga gtgctggcgg cttcagggct ttccggactg ggcttttgat cgagcttctc
    10321 aagtcaattc agatagtcag ttgtataagc aggctggaaa ttctgtaaca gttccggtca
    10381 tttttgatat tgctagaaga ttaaaggagg taaaaacaga tgatttatga tgagttgatt
    10441 ggggaatcta ctgggtgatt ggcaagattc agtcagatcc ggaattggaa gaagaactgt
    10501 accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccctgatgat ccgtatgtaa
    10561 ttgatgagga aacggatgct cgaatggagc tgaatcaagt catcagtgaa ttggaacgaa
    10621 ttgcggactt tgctaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt
    10681 tggaaattga gggtattacc gcggagtaac ggccaaataa accactgata ggcactgacc
    10741 gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgtttatg gacggcgtta
    10801 gccggccatg atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaataa
    10861 gcaagcaacg ataaggaatg gctgtcctat caaggataat aagcaatttg tgtagatgtt
    10921 aactgatttg aaaaagttaa caccttaaat ttggaggtga gaacttggca caaaggaatt
    10981 tagattatcg gctattaaaa gaccggcgta atgaatatgg cgtttcacaa aataaattag
    11041 ctacgacttg tggacttagt cgcccgtact taaatcagat agaaaatggt ggcgtaaccg
    11101 catcgacaaa aaccatgagg aaaatttttg accaactgga aagtttcaat cccgagttac
    11161 ctttaacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat gcgaggaaaa
    11221 tcattcaaga gattctccat ttgaaatttg attatatgct ccacgaagat tacgcctttt
    11281 actcctatca agaacaatat gttatgggag atattgtggt gatgttgtca catgaagaag
    11341 ataaaggcgt ccttttagaa ttaaagggtc gtggttgtcg gcagtttgaa acttttttac
    11401 tcgcccaaaa gcgtagctgg tatgactttt tcgaagattg tctaaaagcc ggtggcgtga
    11461 tgaaacgctt agacctggca atcaatgatc gagttggcct gttagatatt cctgatttaa
    11521 cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agctatcgtt
    11581 ccggggaact gttgaaacct gatgataaag atggtatggg caatactttg tatattggca
    11641 gtcttaagag tgaggtctat ttctgtttgt acgaaaaaga ctatgagcaa tatatcaagt
    11701 tagggattcc tttagaccag accgaaacga aaaatcgttt tgaaatccga ctgaaaaatg
    11761 atcgagctta tcatgcaatt caagatttgt taaaaggtcg cagtattgaa agtaccacat
    11821 tttccattat taaccggtac ttgcgatttg cggataaggt ggaaggcaaa aggcgaacta
    11881 actggccctt gaatgaacaa tgggggcgct tcattggacg gaatcagaag gaaattcaat
    11941 taacttctga accgaagcct tataccattg aacgaacgtt gaactggctt ggacgacaag
    12001 tcgcccctac gtggaagatg gccaaggagt tggatcggtt gaatcagaca acgtacattc
    12061 aggatatggt aaataatact cgactatcag accggcacaa aaagattttg gagcaacaaa
    12121 gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtgatggaaa ttgtgaaaga
    12181 tattttattg gtgtttggtg gcagtatgct tggtgttact gtgatgtgct tgatgcatgc
    12241 aagtgcagcg gcggaccgag caatggaaaa gaaaggaaag tgatgaaatg aatttcggtc
    12301 aaaattt
//
LOCUS       DACDBG010000079        12247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDBG010000079 DACDBG010000000
VERSION     DACDBG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.079"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..843)
                     /locus_tag="IVZ90_002208"
     CDS             complement(<1..843)
                     /locus_tag="IVZ90_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8263232.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIK"
     gene            complement(1048..1398)
                     /locus_tag="IVZ90_002209"
     CDS             complement(1048..1398)
                     /locus_tag="IVZ90_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263233.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1993..2439
                     /locus_tag="IVZ90_002210"
     CDS             1993..2439
                     /locus_tag="IVZ90_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8263234.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            3063..4055
                     /locus_tag="IVZ90_002211"
     CDS             3063..4055
                     /locus_tag="IVZ90_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8263235.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            4071..4955
                     /locus_tag="IVZ90_002212"
     CDS             4071..4955
                     /locus_tag="IVZ90_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8263236.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            5004..6611
                     /locus_tag="IVZ90_002213"
     CDS             5004..6611
                     /locus_tag="IVZ90_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8263237.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6612..8096
                     /locus_tag="IVZ90_002214"
     CDS             6612..8096
                     /locus_tag="IVZ90_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8263238.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            8273..9265
                     /locus_tag="IVZ90_002215"
     CDS             8273..9265
                     /locus_tag="IVZ90_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8263239.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            9317..10186
                     /locus_tag="IVZ90_002216"
     CDS             9317..10186
                     /locus_tag="IVZ90_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8263240.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(10606..12171)
                     /gene="guaA"
                     /locus_tag="IVZ90_002217"
     CDS             complement(10606..12171)
                     /gene="guaA"
                     /locus_tag="IVZ90_002217"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8263241.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         3799 a         2039 c         2594 g         3815 t
ORIGIN      
        1 ctttattttt gtataatgaa cggtactttc ttgaaaatcc atcacttgtt caggacttcc
       61 caactcattt aaagctaaag cattgtaaaa aaatggacgt ctaactatat attcgcttgt
      121 atatagtata tcttgattcg tggaatcata gggtttcttt accatctttt tggtgacagt
      181 tggtagtttg agattccggg acttgaaaaa agcttttttc ttatgctctt tcaattccag
      241 ctctttatcg aaatatttgc tgatatagcg cccgcgattt tcgatactgt ctacatcgat
      301 cttgttgatc ttaacgaaac cctgacccca aatcgtagtt aaccggttaa atgagataaa
      361 ggggaaatca aacaagataa tatggtagtg gattgcaccg cgttgctgct tttcccacgt
      421 ggcgagatac tttaaatttg cttttttagt tttatagagg tcataattca gtcgtttgat
      481 aaatttttta aattcattat ttgcatactc aatgtcttga atgttttctt tgaaagtgag
      541 agttaaaaat ttcgtttgat tgtcaaaatt cgtgtctaca aggcgggcaa tctcaaatcg
      601 catactttga taatgtttct gtttgcgttt taaactgtca tattgtcctt gagcggataa
      661 ttcatcatag ttttttcttt tttcaagagt aatatcattt tcataaatcc aagctaattt
      721 ttgttttcgt tccagattta cttcgttttc gatcgtttta cgtttgggag taatgattgc
      781 ttcataatca taaatttcaa tatacgtggg agtttcaatg attttctgat tataagcttg
      841 caaaaatcat cactccttgt tgcgtattcg attgtcaaag aaccagtcat tttctcctga
      901 tgttgatcta taaatcaagt taagggggaa ggcctgcgta gcagcccttc tagcctatgg
      961 ggtgttgggc ggtcttaacg gacagaaacg gctctgtggg cgctgccccg ccgtttcgtc
     1021 ctaaccaccc aacactccat agtatagtta gttttgtgta gtggtacctt gttttctggg
     1081 aatcaaagtt gccgtatcag cggttgccga taacccataa ctgacaaatt gtccattcga
     1141 gcgtccccaa ggtacaatca ccaaattggt aaatgtaatt atggctaaac cttgttgggt
     1201 aagttcttcc ggcgtgatca caggatgttc agatttgact ttgacactga ttttttcgta
     1261 gcgaagtttt ggtaggatac attcgtagct ccaacctaga atttcgccct ccttttgcca
     1321 gcgagtgaca ttgacgactt ggtattcttt ttcgagtgtt tcttttggta aactgagatc
     1381 ggtaattttt attggcatca caattctcct tttttatacg aaagtttata cgatttgatt
     1441 aaaaaatata gatggccatc tacgacttgt attatatacg agaacttata cgtagtcaag
     1501 agattttcta aaaaaatata tacgattttt tatacgagtg ttataatgaa aaaaatgata
     1561 aggaggaatt tgaatatgtt tggtttagta caacctaata aagtgaaggt gggccaacga
     1621 atcaaagaaa taaaagacgg aatgtctctc tcttttacag aattagggaa tcgattaggt
     1681 ttaaaaaagc cgacgatcag ttcctacgtt caaggctatg cgttggctcc taagagtgtg
     1741 atcaaccaat tatctagtat cagcggaaaa cctgtcggct ggttttattt tgggcatgtg
     1801 gaagaatata tccgagagta tttgtttctc attggtcaga aaaaaatagt ggacgattat
     1861 cctgaaatca ttgagaaaat aaagcaagaa ttttatacgg gcgagttcaa aaatcctgct
     1921 tgggagaatg agctaggcta tcctttggag gaatttatcc aagaatgttt tgtagaatat
     1981 tcgagtgaaa tcatgaagga ctatgtcgta cagattacga aagaggtact tgaagaatcc
     2041 aaaacgctaa acaaactatc atcaaaagca aaagaagaag ctgtcacgat tctttcatct
     2101 gggatcattg actatatcga gatgagtggt gagatcaagt atggagagaa agatcgcatt
     2161 gcgaaaatgg tgaaagaatc aataaaaacc tttgatgtga aacaagacct gacatttaat
     2221 gattcctatt tgattggaaa gttgatcaat attcttggta cagatgaaga tacggagctg
     2281 tttctttctt ctttatcaat gatatttact gataagcatt tttccactcg atttggtggg
     2341 gaagaattag tcgaaatatt tcaatcgatg cgaccagcgt taatcaaact ctatcgggag
     2401 aaaacaccag atgaatttta tgattggttt gagaagtaaa ataagaaaag tggcttagat
     2461 tacaagttgg tgaaaaaatt tttttcagca taacatcagt aatctataaa ggggttgttg
     2521 attatactgt agaagttaag tactcaagag attttttgta cgtaaacaaa gcaaaaaagg
     2581 gtgcagtttc actagaaaat gtactctttt ttgtgatttt tcttagttat agagtttttt
     2641 tgaaacttta aaaatgagaa aacatattga ttttaaagag tgtagacata gcttcttttg
     2701 ctaaatagat taaacttact ggtgagtggt cttgaacata cttttaaatc acattttact
     2761 gagttttatt ggattttata attcattgga aacctcgtac ttaatctgtt tttatctatc
     2821 gactagaatc tgtatatatt ccagctatca atgaggccgc agaaaagggt gcttaaaaat
     2881 aatctgttgt ttaaaatatt attatcagaa tacttagata caaaattctg gcacaaagta
     2941 aaaaaaatat tgacttaaaa aaatggatat ggtaatctat aaacgaaaat agtggtaacg
     3001 tttccaaaat gcatcttcgt taaattttta tttttttaaa cgaaaatgga aacgtttcta
     3061 aaatgtattg ggaggagatt gaattggaaa agacattggg accaggtggt gagataaggg
     3121 cggtacaacg ggataaaaaa aatatttcga agtgggaaaa attaaagaaa cagttgcggc
     3181 gtgataagtg gatgtacttg ttgctcttac caggtatcat catgacgttt atctttaagt
     3241 atgtgccaat gtacggtgct gtgattgcat ttaaagacta taatcctatg ctaggaattt
     3301 tggaaagtcc ttgggtagga ctagatcact ttagggaatt tatcagttca ccaaatttta
     3361 cgcttttgct gagcaacaca ttaaaaatta gtatttatgg tttattgtta ggtttttttc
     3421 caccaattat tttagcactg agcttgaatc aaattatcaa taaaaaaata aaacagaaaa
     3481 tccagttgat tctatatgcg ccgaatttta tctcagtagt tattattgtg gggatgctgt
     3541 ttttgttcct atccgcaaat ggacctatta atgggttagt agaaaaggtt acggggcaac
     3601 caattatgtt catgtctaat ccagcttatt tcagaacgat ttatatttta tcgggaattt
     3661 ggcaaggaat cggctggtca tccattcttt acaccgcaac gctggcggga gtcagtaccg
     3721 atttatatga ggcagcaaat atcgatgggg caaatatttt acaaaagatt tggcatatcg
     3781 atttgcctgc attgaggcct gtgatggtga tcagcttcat cttggccgcc ggcggtatta
     3841 tgaatgtagg atatgaaaaa gcctacttga tgcaaacttc aatgaacatc cctgcctcag
     3901 aaatcatttc cacttatgta tacaaagtcg gcttgcaatc aggtgattat gcctactctg
     3961 ctgctgttgg tctgttcaat atgattatta atgtcgtttt attactgatt gtcaacaata
     4021 taaccaagcg gctaaacgat ggtcaaggct tgtaatgaaa ggagaaaatt atgaatagtg
     4081 ctttatcaaa aatcgaccgt caaatcttat ggttgaatcg agtgattgta acttttttag
     4141 tcgtcatcac cattgttccg ctgatttatg tactggtagc ttcttttatg gacccagcta
     4201 ctttgatcaa taaaggtata agcttcaatc cgaaagattg gacaatacag ggatatcaac
     4261 gggtatttgc agatgattcc attttacgcg gttttgtaaa ttcgttgttt tactctttta
     4321 gcttcagctt attgactgta tttattacga tgatcactgc gtatcctttg tctcgaccta
     4381 acttggcggg gaaaaagatt attatgactt tttttgtcat tacaatgttc gtgggtggtg
     4441 gcttgatacc tacctattta ttggtaaaga acttgggcat gttagatagt gtatgggcga
     4501 ttatcgtacc aggtgctata aatgtgtgga atattatctt agcccgtacc tattttagtg
     4561 ctttgccaaa agaactttct gaagctgcta ctatcgatgg cgccaatgac ttgcaaatac
     4621 tgttcaaagt catgcttcca ttagcgaaac caattatgtt cgtattgttt ttatatgcct
     4681 ttgttgggca atggaactca tactttgatg ccatgattta catcaaagat cccgacttac
     4741 agccgttgca actcgtatta agaaatattt tgatccaaaa ccaaccttca caagacatgg
     4801 tgggagccaa tactgccatg gcagaaatga aacaaattgc tgagttgatc aaatacgcaa
     4861 cgatcgtcat ttctagtctg ccattaatca taatgtatcc tttcttccaa aaatattttg
     4921 ataaaggtgt tttagttggt tctgttaaag ggtaagtatt gttcactatt tgtatgaata
     4981 aaaattataa aggagatttt aagatgaaag caaaatggat gctgccaatc gtttttactt
     5041 ccgtactaat tttgggggct tgtggaggct caaaagaaaa gagtaaatca agccctgatt
     5101 atgaactaga aaatgtctct tttccattga aagaaaaagt atctttaaaa atgatgtctc
     5161 aaagtgcccc tttggctccc aatgatccta atgacaaatt gattttccaa cgattagaag
     5221 aagagaccaa tctccatatt gattggacaa actacagtag tgattttgcc gaaaaaagaa
     5281 atttggatat cgctagtggt gatttaccag atgccatctt caacgccgct gcaggtgact
     5341 atgatttatt aaactgggcg gaaagtggcg ttattgtacc gattgaagat ttgatcgaag
     5401 attatatgcc aaatctgaag aaaatttttg atgaaaaccc cgaataccga caaatgtcta
     5461 ctgcacctga tggtcacatt tactctatgc cttggattga agaattaggc gaaggaaaag
     5521 agtcgattca tacagtaaat gggatggctt ggatcaacaa ggagtggttg gataatttgg
     5581 gtcttgaaat gccgcaaacc actgatcagt tgatgactgt gttggaggct ttcaagacgc
     5641 aagatccaaa tggtaatggt gaagcagatg aaattccttt tagctttatt aacaatggag
     5701 gaaacgaaga cttaaaattt ttaatggggg cattcggtct aggtgataat gatgaccact
     5761 tagtcgttga taacgatggt caggtacaat ttactgctaa caaagaaggg tataaagaag
     5821 ccatcaaata tttcaatgaa atgaataata aaggtcttat tgataaagag gctttcgaac
     5881 aagattggaa tacgtatgtt gccaaaggga aagaacataa gtatggcgtg tactttactt
     5941 gggataaagc aaatatcacg ggtgcagatg atagttatga cgtcttgcca gtcttagcgg
     6001 gaccagatgg aacgaaacat gttactcgta cgaatggcat gggtttctca agagatcgtt
     6061 tcgtaattac tagtgcgaat aaaaacttgg aacttacagc taggtggata gataaaatgt
     6121 atgaaccgtt acagtctgtc caaaataact ggggaaccta cggtgatgaa gcacaggcga
     6181 atatttttga attaaaggat ggtatgttaa aacatttgcc tttagagggg acagcaccgg
     6241 gggaactaag acaaaaaacc gaagctggag gaccattagc agttcttgat tcttattacg
     6301 gcacagtcac tactatgcca gatgatgcga agtggcgttt agatttgatg catgacacgt
     6361 atttgccata tattacagaa gacaatatct atccacgtgt ctttttagaa aaagaagaaa
     6421 cagatcgatt agcaaaaatc gaaacggata tgtgggatta catctatcgc aaaagagcag
     6481 agtggatcac cggcggtaaa atcgatgaag aatgggaaga ataccttgct gaattggaac
     6541 gtgtaggttt ggaagaatgg attcaaataa aacaaactgg ctatgataat tttttgaagg
     6601 gagcacaata agtgttagta gaagaaacgg cgattttgga taaagcaaat cgatttattg
     6661 aaatgatgcg aggaaaagtt aaccctcagt atcgacaaaa ctatcatttt tccgcaccca
     6721 tcgggtggat aaatgacccc aatgggttta tatattacaa agggcattac catctcttct
     6781 atcaatacta tccttacgat agtcgatggg ggcccatgca ttgggggcat gcgcgcagta
     6841 gtgatttggt ccattgggaa gatctaccag tagcgttagc cccagatcaa ccttatgaca
     6901 gcggcgggtg tttctcagga agtgcaattg aaagagacgg aaaactatat ttattttata
     6961 caggctgtca agaaattgat ggtattagcc atcagcatca atgtttggcg gtctctgaag
     7021 atggtattca ttttagcaaa tacagtggta atcctgtggt agcagaaaaa cagctaggtg
     7081 aaaatgggct aatacaagaa tttcgtgatc ctaaagtaat tgttcacaac gatggttact
     7141 atatggtagt agctactaaa tctacacagt ctactggtag aatagtgatg tttcaatcgt
     7201 cagatttgta tacttggcac ttttactctg tgctgctaga agggacttca gagcaaggaa
     7261 taatgtggga atgtcctgat ttattccatt tagacggacg agatgtctta atcatgtctc
     7321 cgatacaaat caagaagaac ggactggaat atcataatat cagttctacc atggcttgta
     7381 ttggtgaggt aaactgggaa acaggtcaga tgatagttga taatttccat gaaatggatt
     7441 ttggaatgga tttttatgca ccacaaacaa caagagatcc taatggtgga cgggtgatga
     7501 ttgcttggat gcagatgtgg gatcgtacat tgcctacaca tgagttaggc catctttggg
     7561 caggctctat gagtttgccc cgagagttaa gagtgaaaaa aaatcgattg gtacaaaaac
     7621 ccatctcaac tgtatatagt catttagttt atcaacaagg cagtgaaaat attgcagtga
     7681 ctgacacatc agtcgtattt cgaaacgcgc tgtctgattc ttcatattta catctagtcg
     7741 cagatttgga gaaagctgaa gtttttacaa ttgaattact gcggtctaag acggattgtc
     7801 tcaaactagc ctatgacact gctacacagg tttttgaatt ggatcgtcag aaactaggaa
     7861 gagagcttac aggggcggaa gaagaccctc tgaatgtacg aaaagttgaa gtacctttga
     7921 ttgataatca gctcgttctt gagattttta gagatacctc atcaattgaa gtctttatta
     7981 atgggttaga agttatgacg gctactttct atgagctgga aaaagctcga gatatcttgt
     8041 ttcattcgat cggagaaagt cgtattcaag cttttgaaac agcgagtgtg aaataaatgt
     8101 ttgattgctt aggcgatgaa tcaattattt aataggtttt gaaggataat gtttttagaa
     8161 aagaacagat agctagattt cccatggatt tgctattagt attttgaaaa aattagtaat
     8221 ttatgacaaa attcattata ctaaacttgt gcagaaatgt gagggatcaa ctatgaaagc
     8281 aacaatgaaa gatgtcgcaa aattagcagg tgttggagta ggaactgttt cccgggtatt
     8341 gaataacggt tctgttaaag cttctactag agaaaaagtt gaagcagcaa tgacagagtt
     8401 aaactatcaa ccagattttt atgcccgggg gttaaagatg aagcatacga acacggttgc
     8461 tttgattcta cccacggtat ggcatccatt ttttggcgaa tttgcctacc atgtagaaac
     8521 agcattagaa agacaaaact acaagcttta tttgtgtaac tccagtggta atagtgaaaa
     8581 agaacaagaa tatatccaga tggtcagcca aaataaagta gacggcatca ttggaattac
     8641 ttattcagat atcgatgaat acatttcgag taatctgcct tttgttagta ttgaccgtta
     8701 tttcactgaa gaggtgattt atgtgaccgc tgacaatgcg gccggtggac gtttggctgc
     8761 aaatgaactt tatcgccgag gatgtcagaa tgtagcatat atcggtgggt accaagaaac
     8821 accgaatgaa acgaagagtc gtcgaaaata ttttgaaatc gagtgccgtc aactggggat
     8881 ttcctataaa gcgttagata tgttagaacc tttagaccat ctctcagaac gtgtaacaaa
     8941 ttttttgtta gagaatcccg atatcgatgg gatttttacg atcaacgatc caatggcatt
     9001 agctgtcatt gatatgcttg aaaaaatagg gaaaaacgta ccagaggatg tccaagttat
     9061 cggatttgat ggtcaaaaga tgacagaagg agatccttat ctagtctcaa cgattgccca
     9121 acctacaaag caaatggcgg aagtggcagt ggcaaaatta attgctgtca ttaatggaga
     9181 gacagtagaa aaacgtaccg tattgccgat tgaatttggc gaaggtgaca caacgaaacc
     9241 tttagtgaga aacgattttg cttagtgcaa atacgtttct ttttttgaaa atgagtgaat
     9301 gaaaggtgta gataaaatgt tatatggtgg aatagaagcc ggtggaacaa agtttgtctg
     9361 tgggattggt aatgaacaat tacaagtgat agaacggaaa agttttccta ccacaacacc
     9421 agaagaaacg atggagcaag tgtttgattt ctttgataag tacaaaggaa atatcgcatc
     9481 aattggtgta ggagcatttg ggcctattga tattcaaatc gaatcaagaa cttacggata
     9541 tatcaccaac acaccgaagt tagcttggca aaattatcat tttgtaggag ctttggaaaa
     9601 cgccttgtcg gttcccatct attggactac cgatgtaaat gctgcttgtt atggagaata
     9661 tatggcaggg tatggaaaag gtaaaaatag tgttctgtat tacaccgttg gtacaggtgt
     9721 tggtggtggt gctgtagtgg atgggaaaat agtcgcaggt tttagtcatc ctgaaatggg
     9781 acatatgctc ttacaacgtc ataatgaaga tcaatttgaa ggaatctgtc cttttcatca
     9841 tgattgtttg gaagggttag catcaggacc tgcgattgaa aaacgctttg gaagaaaagc
     9901 ccagactatc tcatttgatc atccttattg gacgattgag gcagattatc ttgctcagtg
     9961 tgtgtataac acaacgttac tatatgcacc ggaagtaata attttaggcg ggggtgtaat
    10021 gaaacagccg caactactcg agttgattcg ccaatcgttt aaagaaaaaa tgggttccta
    10081 cgtggatacg cctactctcg aagaatatct actaactcca aggcttgctg acaatgcggg
    10141 cttgattggc tgcctagctt tagcaataaa gaaactcaat cattagaata gtatttatta
    10201 gaactattca taacataccg gaagtttaaa agggtattct gaaacttaaa tagtctcaat
    10261 tcattcttct ttcttgagaa tctgagtaga gacttttatt ctgtttcaaa ttaatataat
    10321 tacgaaatga tattgagtac aagaaagttc tttttaccag atcttttaaa tcgaaaaatc
    10381 tgatatatat atttatagta ctaaaaatct gattagataa aaagctaatt caataggaaa
    10441 ataaaggtag tgaataaaag caaagccctt gttagtagtt aatctaacaa gggctttggt
    10501 agggaaaatg gtaggaaacc atcttatttt ttacgaaaat caggaacttt agtaattgaa
    10561 taatgttgat ttataggtat tcctcatcca tattatgttg gctctttatt cccactccac
    10621 tgttgcaggt ggtttgctgg taatatcata cacgatacgg ttaacgtgtg cgacttcatt
    10681 tacgatacgg acactgattt tttgcaagac ttcccaaggg atacgggcaa agtcagcggt
    10741 catgccgtca atagaagtga cagcacggat acctacagtg tagtcatacg tacggccatc
    10801 tcccataacc ccaacagagc gaatgcctgg taggacggtg aagtattgcc agatgtcgcg
    10861 atctaaaccg gcattggcga tttcttcgcg caagatagcg tctgattcac ggacgatttc
    10921 taatttttct tctgtcaatt caccaaggac acgaatacct aagcctggtc cagggaatgg
    10981 ttggcgccaa acgatgctgt caggcatgcc taattgggta cctaattcac gtacttcgtc
    11041 tttgaacaat gtgttcaatg gctcgatcag cttgaattgc atgtcttcag gtaatccacc
    11101 tacattgtga tgtgatttga tggtttgggc agtttcagtt cctgattcga tcacatcggt
    11161 ataaagggtt ccttgtgcaa ggaaagaaac gccttcttca ccggcaagtt tggttgcttc
    11221 atcatcgaag acatatacaa attcatttcc aatgatttta cgtttttctt ctggatcaga
    11281 cacgccagca agttttgata agaaacgttc tttggcatcg acttttataa tgttcaagcc
    11341 aaattttccg ccaagacttt ccataacttg ttctgcttca cctttacgca acaatacgtg
    11401 atcaacgaag atacatgtta attgatcgcc gatcgctttt tgtaaaagaa caccaacaac
    11461 agaggaatct actccgcctg aaaggcctaa taaaactttt ttgtcaccga cttgttcacg
    11521 aatttttgcg atctccatgt cgatgaagtt atccattgtc cagtcaccag tacattggca
    11581 gacatctagt gtaaaatggc gcaacaagtc atttccatat tcggaatggc gaacttctgc
    11641 atggaattgg attccgtaca ttttgcgttc tgcattttgg atcgatgcga tggggcaatc
    11701 tttgctggta gcaacgacat cgaaatcagt cggtgcttgg gtaactaggt caccatgact
    11761 catccaaacg gtttgttttt caggagtgtt ttggaaaagg gccgcttctg cagaagtgat
    11821 ttccaattct gcttttccgt attcacggtt tttggctggt ttaacttttc cgcctaattt
    11881 ataagtaatc aactgcatac cgtaacagat tcctaaaact gggatcccca aatcaaaaat
    11941 cttttcatca ataccaaagg ccttctcatc ataaacactg tttggtccgc cagaaaggat
    12001 gattccttta gggttcattg cctttacttc ttctgcggta atgcggtggc tcatcaactc
    12061 tgagaaaaca ccgaactcac gaatacggcg agtgatcagt tggttatact gactaccgta
    12121 atcaagtacg ataatttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
    12181 aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
    12241 cagacct
//
LOCUS       DACDBG010000080        12161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDBG010000080 DACDBG010000000
VERSION     DACDBG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.080"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            151..1089
                     /locus_tag="IVZ90_002218"
     CDS             151..1089
                     /locus_tag="IVZ90_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8263242.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            1101..2012
                     /locus_tag="IVZ90_002219"
     CDS             1101..2012
                     /locus_tag="IVZ90_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8263243.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            2036..3508
                     /locus_tag="IVZ90_002220"
     CDS             2036..3508
                     /locus_tag="IVZ90_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8263244.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            3559..4170
                     /locus_tag="IVZ90_002221"
     CDS             3559..4170
                     /locus_tag="IVZ90_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8263245.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            4200..6113
                     /locus_tag="IVZ90_002222"
     CDS             4200..6113
                     /locus_tag="IVZ90_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8263246.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            6241..9222
                     /locus_tag="IVZ90_002223"
     CDS             6241..9222
                     /locus_tag="IVZ90_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8263247.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            9219..10880
                     /locus_tag="IVZ90_002224"
     CDS             9219..10880
                     /locus_tag="IVZ90_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8263248.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            10920..11852
                     /locus_tag="IVZ90_002225"
     CDS             10920..11852
                     /locus_tag="IVZ90_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8263249.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         3991 a         1924 c         2515 g         3731 t
ORIGIN      
        1 acagggctac ttatgattgt gaatacagtt tccgtttaaa aactattcta ttgtagaggt
       61 ttaataaact aaaaataatg tataaaaaat taatgtaagc gattaaccaa gtgggtataa
      121 tcaaggaaac gctgagagag gagagacaaa atgaagctaa agaaagggtt attaggaaat
      181 ctaaaaaaaa accgagcatt gtttattatg cttttaccag gtgcgttatg gttatttata
      241 ttttgctata taccaatgtt tggagttgta atcgctttta aagatttcag aatttcatca
      301 aggggattct ttacaagttt gttcaacagt ccttgggttg gctgggaaaa tttcaaatat
      361 ctctttgctt cgcaagatgc ttggatcatt actcgcaata cggttgttta taacgttgta
      421 ggtattttgc taagtacttt ttgtacagtg tcattagcaa tcattctgag ccaaatggcg
      481 aacaaacggt tattgaaaaa gatgcagaca tttatgattt ttcctaattt tttatcatgg
      541 gtagtcatca gttactttgt ttacgcattt ttgagtacgg ataaaggatt aattaatatt
      601 ttactaagca attttggtgt ggaacccata aattggtata gtgaaccgaa gtactggcct
      661 tttattattg tttttacgag tttatggaaa ggcgtaggct atggatccat catttattac
      721 gcgtcaatta tggggatcga tactagttat tatgaagctg ctttagttga tggtgccagt
      781 aaatggcaac agataaggaa tgtagtcttg ccacatttaa gatcgattat tgtcattatg
      841 ttgattttgt ctgttggtag tatttttcga gctgattttg gattgtttta tcaattgcca
      901 agaaattcgg gggctcttta tgatgtgacg aatgtattag atacttatgt ctaccgtgga
      961 ttaatgaata gcggagatat cggaatgtct acggcagcgg gattatatca gtcatcagtt
     1021 ggctttgtct tgattttgat tactaataag atagtcaaca agttagatcc tagttcagca
     1081 ctattttagg aggaatcaaa atgatatcaa aattaaaacg gcagatgtac ttttcgctgc
     1141 cagtcgaacg gtttttcaca atattactaa tcttatttac tcttagctgt atttttccct
     1201 ttattttcgt aatcgtcatt tcattaacag atgaaaaagc attacaactc aacggttacg
     1261 aattattgcc ggctcaatgg agcactgatg catatcaata cttaatacaa gatggcggac
     1321 agttacttcg gtcattaggc gttacaatta tgattacagt gattggaacg ttgattactg
     1381 tttttatgac gggaacctat gcttatgttt tatcaagagc ctctttccct taccggaagt
     1441 tctttacttt ttatcttttt tttaccatgt tatttgcggg agggatggtt ccttcttact
     1501 tagtgatgac gaagatgctt ggcttaaaaa atacaatttg ggctttgatt ctacctcttg
     1561 cttttagccc atacaatgtc attattttaa ggacattctt taagaagtcg attccagaat
     1621 cgattatcga atccgctaaa atggatggct gtagtgagtt tcgagttttt ttccaaatcg
     1681 ttttgccttt agcgatccca ggggttgcaa caataggttt attttcaagc ttaggttatt
     1741 ggaatgactg gttcaacgca ttgctgtata ttgacaccaa caaattgatt ccattgcaat
     1801 atttgttaat gaagattcaa aattcaatgg aattcttagc aaataataat gacattacat
     1861 tagcgcagca acaagcaatt caaaatagcc ttcctcaaga atcaacacga atggcaatgg
     1921 tcgtagtagc tactttaccg attgcaattg tttatccgtt cttccaaaaa tactttgttc
     1981 aaggattgac gatcggtgga gtgaaagaat aaaaaagaat aggagatgtt aaaagatgaa
     2041 aaagaacatg cagaaatatt tgattggtgg tgcttctgta ctaacccttg gcctattagg
     2101 tgcttgtggt agcagtaatg gcgaagcaaa taatgaaaca ggaaccacaa aagttacttg
     2161 gtatcaggtt ggagaagaac ccaaagattt tgatgaggtc atgacaaaag tcaacgaaaa
     2221 attaaaggaa gaaatcaatg tagagttgga tatgcggctg attccggacg gggattatca
     2281 gcaaaagctt ggtgtgatga tcaattcagg agaagaatat gatatttgtt tcgtcaatgg
     2341 cacagattat gtgaattatg ggaacaaagg tgctttcatt agtctcaacg atcatttgga
     2401 taaagagttg aaagattatg cagctgaatt gaatgaagga tttattgaag gtggggcaat
     2461 taatggcgaa acttatggat tgccagtcaa taacgatatt gccacaacgg aatattggac
     2521 ctttaataaa gatttggtag atcaatacca attggatgta gagaatgttg aaggtgtaga
     2581 aacattggct ccagtattag aaaagtttgc agaaaaaaat actgatacga atattacgcc
     2641 aatcggtatg acccgtacca gtgtctgtat tccttatgac aatattatcg gtggtggtgt
     2701 accgctagcg gttgcatttg aaggcgatac acaaacagtt gttaatactt atgagactcc
     2761 agaaatgatg acttatttga aggctatgca tgagttttat aaaaaaggat atattttgaa
     2821 agatgcagcc actgttacga cagatccatt tcgaattgat agtaaaaact ggtttttagg
     2881 tcgtaactca agtggttatc aaccattagc acaaaaaaaa ttagaagctc tagcgggaca
     2941 tggattaatt ttaaaaccag ctggaaaagc ttttaaaacg actggatttg caagagtggc
     3001 aatgcatgcg gtatctgctt caagtaaaca tccggaagca gccttgaaat tgttgaatta
     3061 tgtgaatacc aatcaagaag tagcggattt aatggtttat ggaattgaag gaaaacacta
     3121 tgatcgcaat gaagatggtt cgattacttt aacaaactct gatgattatt atcaaggcgc
     3181 ttgggcattg gtaaatactt atctagtttc aaaaacttca ccgattacag aagaaagtaa
     3241 aaaactagaa gaagaaagta aaaagtttca aaatgaagca ccgatgtctc ctattttagg
     3301 tttctctttt gacgcttctg attttcgaac agaaattgca agtgtacaaa atatcgtgtc
     3361 acaatatgaa gtacttttaa gctcgggaac agttgaccct gaacaatatg taccagagtt
     3421 gttagaaaaa ctagaaaaag ctggattgga taaaattcag gcatctgctc aagaacaata
     3481 cgatgaatgg cgaaaatcta acaaataatt ttttactaaa tgaaaagaat agactaagca
     3541 gttagtgggg ggattcaaat gaacgaaaac aaatggacca aaggatttaa ttgggttttt
     3601 caattgttgt tactaaactt tacttatttt atactaattt gtttaggatt tggaatttta
     3661 ggttttttcc ctgctacgat agctttgatt gctggcgttg tgaatcgaaa aaaatatggc
     3721 ttgcaagagt ctgtgaagtt catgagtaaa atctataaga aaaattttgt tagatataat
     3781 ctatattgct ggtcagcaac tttgttgttg gccggcaata ttactacgat ctggctttgt
     3841 tggcagcaaa gcgcaccatt gttttattta ttaagtacat tattatcggt aattacactg
     3901 ttcctagtct ttgcaatgtt gaattgggtc gtgatcaaaa gtcttgggtt gtggatgatg
     3961 ggagaccgac tgattgacta tttgcttttc acagcaacga tgttgccaac ggtagtcatg
     4021 caaggcttgg tgcttggaat ttatttccga atattgatga ttttgcctgt gatttttctg
     4081 tttggcggaa cagctgtaac ggtaatattg attagtaaat taactgaaag agcgttagca
     4141 aagttgcctt gtcagataca atcgcactaa ttttttcaca aaaaggagta tttctttcta
     4201 tgaagttaac tgataaacaa gtagctaata tagcctttat ccagcaactt gagattccat
     4261 tgacacaaaa agatgaagaa caaatagact tgatttttac tgacacaaag gctaatgtgc
     4321 gacaaatcaa gattactaaa aatgagcagc aacgaatttg cttgtcttat tcacatgaag
     4381 acttgctgta tcgaggattg tttatctgcc ttccacgttt tttagctcaa ggaggagaga
     4441 tgccatacat tgaagaaacc tcgatcattg agaccagtta ttcattagat gcttctcgta
     4501 atggcgtgat gaataaagag actctaaaaa aattcatttc ttacttagcg ttgatggggt
     4561 acaacacatt gtatatttac acagaagata cctatgaagt agaagacctt ccatattttg
     4621 gttaccttcg cggagcgtat acaaaagaag aagtgaatga aattgttgaa tttgcttctg
     4681 gttttggcat agaagtagtt ccatcgatcc agacccttgc tcacttgacc caatttttga
     4741 agtggtatgc tcaacaagaa ctgatggatg atcaaaatac tctactgatc ggtgaagaaa
     4801 aggtttatgc ggcaatcgaa aagatgatta ttgcctgcaa agaaatgtat cagacagagc
     4861 gtattcattt gggaatggat gaagcctatc aagcaggtct gggacgatat ttaagcctgt
     4921 atgggtattg tgatcggaca actttaatga ttcaacatat ggaccgtgtg ttatcaattg
     4981 ttgataaata taagatgaaa ccaatgattt ggagtgattt catttataag ctattagatg
     5041 aaaccggtaa agataggtat tacaatcctg attttaaact caaagcatta gcagctaaag
     5101 gattaccagc aggattaacc tatgttcatt gggactatgg ttcagatgaa gtttcctcct
     5161 atgagaaagt aattgataac catttgaaat tttgtgacat tgatcattac gttttagcta
     5221 gtggtgccca tatctggggg agactagccc cgaatcatgg gaaaacagaa gcaattgttt
     5281 ctgcagctgt aaaagcttct aaaaataaag gtgtacgttc tgtgatgtta actacttggg
     5341 gagatgaagg gcaggaaaca gagcattggc attccttaat tggggcatta aaattagcag
     5401 attatgtcta ttctgaagaa gagtggaatg attttgagga aatctacgat cgtttattag
     5461 gaacaggtag ttatcaatta atgtccgaat tacgatattt cgatgaagtg gataccatta
     5521 caaataacaa cttacaaatg gcttccgtga gtaaactgct actttggcaa gatccgttag
     5581 ttggtatata tgactttcat gtagccgctc ataacaaaat tgccactaga acaattggta
     5641 cttattatca tgagttagct cagaaattag atatgaataa aaattggaat ccaaaacaac
     5701 caattttaac aactattcgt caacgatata tacttttagc tgaaatttta gcacaaaagg
     5761 cagaactagg catctattta caacactgct gtctggtaga agaccttgca ggattaatgg
     5821 aattagcgaa tcggtgtgaa gcactagcca atcgagtaaa tgaattggca tctattcatg
     5881 aaataatttg gtatcaaaca tacaaaacct atggttggga agtattagaa caacgatatg
     5941 ctgcattagc tcatcgtctg aaaaccgttg cgcgtcgtat taaaaatcat cttcaggggc
     6001 aagatgccct agaggagctt ttggaagaac gattaccgtt ttgtcaatat caagtgccgc
     6061 ttgaaatttc tggctttaat tatcggcaaa gctcggtgtc aggctacaat taacgaagga
     6121 atcttcccgt tgaacggacc acttgctcat ctaatgggaa agtggttcgt tttctattca
     6181 taagtagctt accactaact cttttattaa gaagagaaaa aaagtaggaa gtgagaaaat
     6241 atgttatcgg actattatca aaatctgtca gttataagtg tcaatacaga accattacgt
     6301 aattattata ttccttatgc aagtaaagaa gaagcaaaac aagagttaga aagggaaaaa
     6361 acgagccagt ttatcgatct caatggtaca tgggattttc agtattatcc ttctgtccaa
     6421 cagttaccgg aaaatttttt tcaagaggcc tttactgaaa gctatgatag aatttctgtt
     6481 cctggagttt ggcaaacaga gggatttgac ttacctcagt atatcaatgt atcttatcca
     6541 attccttttg atccgcctta tgtacctaat gatacccctt gtggtgtata tcggcgtcag
     6601 tttcaaattg agcaaacaga tgcacgcttt tacttaaatc tagaaggagc agatagtaat
     6661 tgttttgttt ggataaatag aaactttgta ggctatgacc aagtgagtca tagtacatct
     6721 gaatttgatg tgaccaagta tttacaggtt ggggttaatg aaattcaaat tctagtgatg
     6781 aagtggtgtg atggtaccta ctttgaagat caggataagt ggcgaatgag cggtctattt
     6841 cgtccagtgt atcttcttca acgaccaact atgcatctga caagttatca actacggacg
     6901 ttgactgatc ctaaaaaagg aagcggaaca ttaatggttt cttttcaacg atttactcct
     6961 attgaagtat cgttacctat ttcttacgaa ttattccatc ctgatggacg tctcatagcg
     7021 aaaggggagt gttactcgga acaacaaata aatttggatg ttgaagattg ccttttttgg
     7081 acagcagaaa cgcctaaatt atatacatta cttttaacat gtaatggtga atattttaaa
     7141 gaaaaagttg gctttcgagc actgacgatc aagaatcgta agttattgct gaacggaaca
     7201 cctattaaat tatatggagt caactatcat gacgtgaatc agcaaggcac gccagtgatg
     7261 acccgagagc agttacgtgc agatttgtta ttaatgaaaa aattaaatgt gaatactgta
     7321 cgaacggcac attaccctaa atcaccagat ttttatgaat tggcggatga gattggtctg
     7381 tatgtaatca atgaagcaga tttggagtgt catggtgtgg ttaatctata tgatactgaa
     7441 gcgaattatc aactaattgc taatgatccg acattcgaaa agccgattat ggaccgagtg
     7501 atccgttcta ttcttcctta tcaaaatcga agctctatca ttatgtggtc tatggggaat
     7561 gaatctggtt atggagtaaa ttttgaaaaa gctcaagctt tagcacggaa attggatgat
     7621 tcacggttga ttcataatga acgctcgatt gaaccagtta ttgaccaagt gaatgatttt
     7681 agtaacttgg atgttattag tcaaatgtat cccccactgg aagatgttca tcgtcattgt
     7741 caaagaagtg atactgaaaa accttttttt atgtgtgaat atagccacgc tatgggcaac
     7801 ggtcctggag atcttaaaac gtacttcgat ttgattcaac agtatgattc ctttcttggc
     7861 ggttgtgtct gggagtggac tgatcatgca atcaatagag gaactttaga aacgccatct
     7921 tatttatacg gtggtgattt tggtgaaaaa tatcacgacg gcaatttttg cgtagatggt
     7981 ttattgaccc cagatcggca attaacagta ggtgcattgg agtaccgtaa tattcattcg
     8041 cctattcaac tgattgctgt agatgaggag ctgcaagaag taaccttgag caatataact
     8101 gcttttacag ccggttcagc atttaacctt tctcttgaat ggtatataga tggaaaaaaa
     8161 acagtttatg aagtaatgaa tcctttcata cttgagcctg gcaaaagttg ttcattgccc
     8221 atcacagtac ctacaacgat tcatggtgct catacggttg ctgtttcgct atctgtttat
     8281 aaaaatgatg agctttatgg acagcaacag ctctttctta aaaaagaaaa aatgagattg
     8341 ccaagaactg ctcatcataa tgatgcacca caaatttcag taaaaggcac agagctgata
     8401 atcaaaggag aatcctttat ttatatatat caattagcaa aagctgcgtt cacttctgta
     8461 aagatcaatc aaaaggaact tttggaacgt cctactcaat tttcaatttg gcgtgcacca
     8521 acggataatg atcgtaactt agtaaacaaa tggaaagcag ctggatatgc tgaaatgtct
     8581 ttagaaatca gaaaaaaaga ctggaaattc gtagatgatc atctcctgat cgaaacgtct
     8641 tatcgaggag ttattccagg attacagcca atttttacaa tgcacataca gttgaaaata
     8701 aatggtgcag gcgtaatcaa ccaaaaagta tcagtggaga aaaatccgat ttttccagat
     8761 ttccctcgct ttgggatgga ctggccgttg aatgatactt tttcgaaggt ttcttattgt
     8821 ggctatgggc cgtatgaaag ttatcctgat aagcactatg ctgcgatgtt tggtatttat
     8881 gagatggaga ttgaggaatt tgatcagcca tatatcaaac cacaagaata tggtaaccgc
     8941 tggcaatgtg attgggtatc aatcaaaaat catcaaggat ctaccatgac ggtttatgga
     9001 aactctaatt tttctgctct gccttatagt caagctcaat taaccgaaaa gtcacaccat
     9061 tatgaattaa taaaagaaaa tagaacttgg ttcaatttaa gttataaaca aaatggtatc
     9121 ggctcacaca gttgcggtcc ttatttagaa gatcgtttcc gttttatgga agatactttt
     9181 gactggaata tagtgattac tttcaaagga gaaaatagat gaaagaaaca aagatcaatc
     9241 aagtcaaaaa aaaattatct cgttatcaag aagttgttta tcgaaaccat tgcttaaaat
     9301 ggtcaaaaaa taagctgaat gttcgtttta atggattatt ttatcgtttc gaacagttag
     9361 aagaagtctg gaaaccggta acgaatttcc aatcatgtcc tgtacagctc ataaatgatc
     9421 caaaagaaac atacgattta tgtataacac acctttataa ccagcatttg aaaaatgacg
     9481 gattttattt aaaggttact aatgacaatc aacttacgat tgaatcagca aaccttcgag
     9541 ggtttcaata tgctatggaa gccttattag agagtttttt ttcggtagga gatcaattat
     9601 tattaccgga attgattctg aaacatgaac catcagtgaa aattcgagga atcattgaag
     9661 ggttttatgg tatcccctgg acacatgaaa tgcgcctctc tcttttttca tatatgaaag
     9721 ataaccaatt gaatactttt atgtatgccc ctaaagatga tgaattatta cgtaaaaaat
     9781 ggcgtgaact ctacgatgcg caagaattag acaaatttaa ggagctttta tctgctgctg
     9841 aggctagcaa tatcgatttt tggtacttaa ttagtcctgg aaatgatatt gatattacgt
     9901 gtgaagaaga cattcaagtt cttctgaaaa agttagaaca actgattgag cttggcgtat
     9961 ttcaatttgg tctattaatg gatgatattg actatcaatt aaaaggtgct gctaaacgcc
    10021 gttttcgaga gccagcattc gctcatgcct atttagtgaa tcgagtagag gaatatctta
    10081 gcactcggtt gtccaatcat gaattagtca tttgtcctac agaatatgat aatcgtcatg
    10141 gttctcgcta tttagcaacg ttaagtcaag aaataccaga acaattaccg ttcttttgga
    10201 ctggcccaag cacgttagcg gcgtcaattt ctacagaaga gctgcaggaa atggcttctg
    10261 tctatcaacg gccgatgctg atttgggata atattccggt aaatgattat ttagaggata
    10321 aagaactatt gttcatgagt ccatatgaga atcgaacacc gaatctttct aaggagagat
    10381 accaggttac aggagtagtt tcgaatccaa tggctcaatt agaagcttca aagtttacga
    10441 tcaatagtat ggctaattac ttatggaact gtgagcgttt tgatcctttg gaaacttgga
    10501 cgtcagtcgt tgaaaaagta gttggaagta aactacaacc agcttatctt accttaacca
    10561 atgcttttcc aaatcattac acaagcagtc tgctttcaga tgaagaatta ctgctagcca
    10621 aagacccaga atgggtcaca aaagaaatgg ctgctttagt ccaagcagta aaatggatcc
    10681 agcaagaaca acatacatct agattgagaa cagaattgga gccttggtta caagcaatca
    10741 cagaaagttg gactttctgg caagaatggc aattaaaaaa agatccaaaa gaagcagaag
    10801 aatggttggc taagaaaaaa acacaccgca taggacgtga tttagtaact gcccatttag
    10861 agcaaattat caaaagttag atatcttaaa aaataaaaag caagaaaaag gagaacaaaa
    10921 tggtcaagag attaattagt gcaaactaca gtgatgtcaa gaaaatgacg tcaaaagagt
    10981 tgaagcaatc catcaaagcg agtgaaggac gggtgattgt ttgtgaaacg gttgctaata
    11041 ctcttccaca atctggagaa attacgaatg cagaagttgc gtcagcattt ggtgcagatt
    11101 tgattctttt aaatggtttc gattgttatc gtcctgcagt ttttggacta ccaaatgtga
    11161 cgattgagaa ggtttttgat ctacagcaaa cgattgaaaa cccgattcct aaattaaaag
    11221 aactagtcgg tcgtcctgtg ggaattaatt tagaacccat tgcccctaaa acaaatatga
    11281 tgagtgagaa agtagaaatc agccaaggaa gagcaagctc tgttgaaacg atacaaaaag
    11341 cagaagaact aggagtcgat tttatttgct ttaccggtaa tccaggcact ggagtaacca
    11401 atttagaaat tactaaatcc gtaaaaatgg caaaaaagta tttttcggga ttgattattg
    11461 ctgggaaaat gcatagcgcc ggctcagaag agccagttgt gaataaagag gcgattattt
    11521 cttatgcgga agcaggcgca gatattttac tagttccttc agtagggtct atcccaggct
    11581 ttttagagca agacctaatt gaagcggttc ggttggcaaa aaaatatgat ttgttaacga
    11641 tgtctgcaat cggtactagt caggaaagtg cccgaccaga aacattgcgg caattagcca
    11701 ttatgaataa aagttgtggt gttgatttac aacatattgg agatgctgga tacgggggat
    11761 tggcaccagc agaaaatatt ttcgaaacct cggttgcaat tagaggaatg cgacatacca
    11821 tcaactgcgt tagccgctcg atcaatcgct aacaaaatta aataaaaaat ggttaaattg
    11881 attatttaag tgggaacata cttgagatat cagctaactt ctgtcacttt aaaaaaggga
    11941 ctgtgacaga agtgaaaata gacaaaaaat atccgaacaa gagactagga tagcagaaaa
    12001 actatcgttt tgcaacgagg accaactgga ccgtaggagc aattctctta ttgttcggat
    12061 atttcgcttg ttcaaggact tatgtctgta gtgccctttt ttggtaggaa aaatggtagg
    12121 gaaccatatc atttttaatg aaaatcaagc atttcaaaag t
//
LOCUS       DACDBG010000081        11659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDBG010000081 DACDBG010000000
VERSION     DACDBG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.081"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(154..1284)
                     /locus_tag="IVZ90_002226"
     CDS             complement(154..1284)
                     /locus_tag="IVZ90_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8263250.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1825..2031)
                     /locus_tag="IVZ90_002227"
     CDS             complement(1825..2031)
                     /locus_tag="IVZ90_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263251.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2047..2661)
                     /locus_tag="IVZ90_002228"
     CDS             complement(2047..2661)
                     /locus_tag="IVZ90_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8263252.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2808..3491)
                     /locus_tag="IVZ90_002229"
     CDS             complement(2808..3491)
                     /locus_tag="IVZ90_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8263253.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3600..4058)
                     /locus_tag="IVZ90_002230"
     CDS             complement(3600..4058)
                     /locus_tag="IVZ90_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8263254.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4142..5905)
                     /locus_tag="IVZ90_002231"
     CDS             complement(4142..5905)
                     /locus_tag="IVZ90_002231"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8263255.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6126..7586)
                     /locus_tag="IVZ90_002232"
     CDS             complement(6126..7586)
                     /locus_tag="IVZ90_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263256.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7794..8720)
                     /gene="glsA"
                     /locus_tag="IVZ90_002233"
     CDS             complement(7794..8720)
                     /gene="glsA"
                     /locus_tag="IVZ90_002233"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8263257.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8743..10170)
                     /locus_tag="IVZ90_002234"
     CDS             complement(8743..10170)
                     /locus_tag="IVZ90_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8263258.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10201..11415)
                     /locus_tag="IVZ90_002235"
     CDS             complement(10201..11415)
                     /locus_tag="IVZ90_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8263259.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3535 a         2412 c         1922 g         3790 t
ORIGIN      
        1 ttcctctagg aaaaaattaa ctagcaccac gggtataata tgattttttg agatactttc
       61 attgcgaatt cctctttcta aaagaacctc tcgcttgaaa aataaaagat tgtactttat
      121 gataattcaa actgagagtt tctcagtttc tctttattgt ttttcaggtc gatagagcgc
      181 caactcttcg gcctgtatct ttgaagaaat attcaacgtg attccgattc ctaaagaaag
      241 gatcagataa cttgttcctc cgtagctaac aaatggcaat ggcacaccag tcattggcat
      301 cagccccgcg atacttccca cgttcattat cgtctgaacg aatagcaaag ttccgaatcc
      361 taaaagaaac aatccagctt gctgattttt acagcgactg cttaaacaaa agattctcat
      421 gcataatgag aacaataaga aaagcacaca caatgcaccg attagcccaa gctcttctgt
      481 aataatcgaa aaaataaaat ctgtttctgt ctctggaaga tacccttttt ttgtgatact
      541 atttcctaat ccccgcccaa atatgccacc attatatagt gcataaaacg aatgggtcat
      601 ttgatagccc gcaccatgac tatcgataaa agaatctctt aaagtcgcga tcctttcata
      661 agcatgatta aacatctgtg gaagccaacc tttatttcct aaatataata ccccgcctgc
      721 tgctccaatc agcaatgcag aaaaagtaac gattagatag atcccttttt taaatggaac
      781 cgccgctgcc caaaatatga cccacgcaat cgaaaggatc atcaatgctc cagcaatctt
      841 tggctgaaat aaaatcagaa aagttatacc tactgtaatc aggaatggtt tttttagatc
      901 ttttgggcta ctatttccgt cacgaaaaaa ccagcttaaa taaaaaatca aaaaaagatt
      961 tgccagttca gaaggctgga attgaatgcc aaacagagag atccaacgtt gtgcgccgtt
     1021 gacagtgaca ccgaatatcc ctattcttac taatactaaa aagaaaatcg ataaggctaa
     1081 accgtatcct gctattttag ggtgaagtaa atagtgtagt ttgactgaac gagctaagaa
     1141 gatcacgctc caactcaaaa atatgaaaat tagttgacgc aataatagac tttttgtatt
     1201 ttcatcggct tgcaataaac gataagaact ggcactgtat acttctaaaa gaccgatcat
     1261 ggacaaagtc aaataaggac ccaaaatcca ccaatcgatc ttttttcacc gcatctcctc
     1321 ctcacacagc aatccacttg tcaggtgagg agcgagtttg tctcatcaaa taagtcagtc
     1381 acaaaaatat aaagaaaagg tcagaaacct tttcgaacaa gaattctgac ctagcatcat
     1441 ctcatcgtcc gagttttagc actatacaac gtaaagaaaa cattcttagc tattggaaaa
     1501 agccttaatc caacagtttc tgttttaccc attggcgact ttcgacgttt ctgggcaaca
     1561 gcctatgttt gtataggagc ctcacctaac aaggttatta ctatattcta tttacgagtt
     1621 catgatagac ttttcctaat gattaggcaa tggaatttga aatgaaatag tgaaatttaa
     1681 caatctttaa tattttttag aaaaaaataa caaataagtt gaaagaatcc cgttgcaaat
     1741 tctttcagtt gcaccaggat tctttgataa ttttttagtt ttatgtgttt ttttatatgt
     1801 tttttcactt ttcggaataa atatttattt gttaaagtaa ttaaacaatc cgctaatcgt
     1861 ttgatctggc atttctattt ccttaccatc taaagtaaca gtagtaacga caaaatggtc
     1921 ttgcaatggc ggaacttcta agtactgccc ttgcagaaaa tcattcactg tttcatattc
     1981 ttttttcatt tcttttgaat ctgtcgatgg tttatagctt atttttaaca taactagtgc
     2041 tcctttttat tcgggacgtg caatcgtgaa aatttctcct attgctgcat aatcatttcc
     2101 tgccaaacgg cttacagcat tcaattttct tggatcgatc cttcctgtct tatcatcata
     2161 gactgtttca tttaaatgat atttctgtac ttctagcaaa aataaatcag ctgttacttt
     2221 atttttattc cgtatctcga catgttcata taaggatgtt tcaaaacgaa cttttgcttc
     2281 tgtcaatgct ggtactgaaa tgattactga atcagttaaa gtcaagtcag tcaggtacag
     2341 ttcgctttcc tcattaggca gattggctgc tgttttattt acttcttcta cgttgtcctc
     2401 atcaaggata tggataacag cttctcttgt ctcaagaata ttctttgctg tgtcttttac
     2461 tgcatcttgt tttctctgga tcgccaagga taagatgggt cgattcgaac tgacgatatt
     2521 gaagtaactg aagggtgcga tattcactat atcttttgct gatttagtcg ttactactgc
     2581 tactggtcta gggatgactg aaccaatcag caacttgtaa ttttcacgtt ctgtcaattc
     2641 tgaaggactc cgttcaaaca tggtttcgcc ttctttctat aaaattcgtt aaaaagcaga
     2701 tcttgaagac aaaatgaaaa gtaatgcttt tttcatcttc ttagaatctg ctttaaacta
     2761 tctatcaatt tttctttttt gtcaagaatc tgtgccgtgc gtgtttttta gttatttgct
     2821 ttacttgcgg cttcgacata tcttttagtg atcaattgcg gacgggtgat aatagatcca
     2881 actacgactg aaaaagcacc taattcatat gctttctcta cttgttcagg agagtcagta
     2941 tgtccttcca taattactgg gatatcgact tgttctatga tttgattcaa taaatcaaaa
     3001 tcaggtgtct gcgtctttcg tgaatattct gtatagcctg ctaaagtcgt agacaaaaga
     3061 tcaaaaccat cttcttgcgc ttgaagcgct tcctcaaaag tcgaaatatc tgccatagct
     3121 aaccgaccgt gttcgtgaat catttggaca agttcagcag ttgttttttc tgtcggacgt
     3181 ttacgagaag ttgcatccag tgcaatgatt tctgcttgcg tctccaaaag ttcttccact
     3241 tctttttctg tcgcagtaat aaacacttct gacccatcgt aatctctttt gataatccct
     3301 attaccggta aatcaacgat tcgcttgatt tcattgatat cttctgtcgt atttgctcga
     3361 ataccaactg ctccgccttc ttttgcagcc aaagccattc tccccataat aaaagaactg
     3421 tgtagcggct cgttttccaa agcttgacaa gaaacgacga ctccgccttt gatttgttcc
     3481 agtgtatcca caatatctcc tccagtaatg ttatataaaa atacagcttc atataaaaat
     3541 gtaacccttt tctattcagg agacaataat gagctatttc tttcagcaga cctttcttac
     3601 taaaagaaaa ttacttcaaa ttcttcgtat acgaccagct tctcttctgt aaattctatg
     3661 ttcaacgctt ctttataagc atgtttaaaa aactcagcat ggactttccg ccaataagct
     3721 aatgtccgat ccccttctcc ttctttccaa gcatgctctt tcgtgacttc actaaaaggc
     3781 aagatggtca catttatatt tttgatgata caaactggtt cattttttcc attcaggacc
     3841 acgtctaatt gaccaacttt tggtaaaggc tcattctctt gtttatacag atcgtaacca
     3901 cttgatgtac cagtctttat cttttttaca actagttcgc tcaactcgtc tgccattttt
     3961 tctgtatccc caaatgaaaa gaaacctaat ttttctgatt gataactagg atatttttct
     4021 tggaactttt tgacatattc ttctacttgc tgatacataa catccacccc tttgcttttt
     4081 ataatgaaaa aaccatccag atactagtat aaacaattat ctggatggtg agcataatga
     4141 attattgaat attcaagaca cgcagactat cttcaacgat ttgttttggt gttaaatgat
     4201 aacgttcata aagctgccct acaggaacgc tatccgtgaa ttctttctct gcaccaaaat
     4261 ttaatacgtt cattgaagaa tcaccataat aacgactgat attttcgcca aatcctcctg
     4321 acaaactacc atcttctaat gtgatgacta attcgtgtgt ttcttttaat ttatctaatg
     4381 tttctacgtc attttctgtg atcgttcctg gattgatcaa cgttgcttcg attccttttt
     4441 ttctaaaagc ttttactgtt tcttttccta attcaagaaa accgcctaaa gctaaaattg
     4501 ctacttttcc tccattattt actgtttgat aggctggaat actataatca tttgcgatag
     4561 ctgtaccatg ttctacacca tgtgtaggaa ttcggattgc tacaggctgc gctgtttgtt
     4621 cgaacgccca atctagcata gagatcatat cctcttttgt tgctggagca agatatttga
     4681 tgttcgggat acttgataag taaggaatgt caaatgttcc ttggtgtgtt cggtcactag
     4741 ctgagatcgt tccgccaccg actaaaataa tcactggatt ctcgttgatc gctaaatcat
     4801 gtgataattg atcatatgcc cgttgcaaga aagtacttga ttggaagacg actggacgta
     4861 ctccctgaga agccaaagca gaagcgaaag tgatagagtg ctgttcagcg atccccacgt
     4921 caaaataatg atctggatgt ttttcttgga atgttttcag tccaaaggcg cctggtatcg
     4981 ctgcattgac tgcggcaata ggagctgtac ttgtttgcat tttcttatcc aaataatcca
     5041 tgatgatcat attaaatgtt tctccagaag ttacttgctt tgcctccccg gtggacagat
     5101 caaatggtac atgccaatga tatttcattt tattatcgat tgccggctga taaccgcgac
     5161 ctttttccgt atggacgtga agtacaatcg gatgatcaat atcttttact tcttcaaata
     5221 aacggatcac cgtttccaga tcgtttccat tatccacgta acgataatca aatcccattg
     5281 ctttaaatag gttattgctg cttttaccgt tggtttgacg aagctcagct aagtttttat
     5341 ataaactgcc atgattctca tcaatagaca tctcattatc gtttactaat acgattagat
     5401 tggattgtaa aacaccacca ttgttcaatc cttcaaaagc taagccacca cttagggagc
     5461 cgtcaccgat aaatgctacc acatttcctg attctttctt cagatctctc gctttagcta
     5521 aacctgttgc taagctgata gatgtagatg tgtgccccac tgtaaaatag tcatgtttac
     5581 tttcatgttg gttggaatat ccgctcactt cacggtaatg tcctttttca aaaccatttt
     5641 ttcgaccagt taaaagctta tgaccataag actgatggga aacatcccaa acgattttat
     5701 cctttggtga atcaaataca taatgaaacg caatcgttaa ttctactaca cctagattcg
     5761 gaccaacgtg cccacctaca tcgctgtcct tttgcaagat tttttggcgc atctcttctg
     5821 ccaactgttt catttcctca acagataatt ttttgacatc tgcggggcta ttgattgcat
     5881 ccaatacatg taatactgta ctcataatac attctctcct ccgtactatc ttcttttaat
     5941 gaaaatagat ttgttacact ttatctattc tactacttag agttgactct aagtcaatag
     6001 cattttaaaa aaatttcaga gaaagcttgt tatataagta gatcttcgca cgaatcagga
     6061 ctcaaataaa aaccttctac agacaattaa aaagctttgt agaaggtctc ctaattaagg
     6121 tctcatcaag aaatatgttt ttttaatagg tgaagttcct gagcaatatg atacaaatgg
     6181 atgtttgcac cttttaatga gattggatag atctttgtct tttcagtttc taatccattt
     6241 tgatagctag cagcatcgca caaaaccact ctttttttcg cacatagaca ttcatcaggc
     6301 aattcttcta atgaacgaaa taaaatagga tttatttcag cgatgccttc ttccgcatac
     6361 aaatgaaatt gggttcgata aaaaaattgt ttgactgaac tttctgaaac aacaaaaatt
     6421 tctatttcct gtctccccgc ttgttcactg ttgatcaaag aaagactttt gacgaatttt
     6481 cgaatgaaat tttcattcca tcttagttgg atttgatttt tctttttcca ctcttccaat
     6541 atttctacaa tttttccgta cagaagttgt tctttttctg atagaagata gatcctttcc
     6601 ggtaaaaata cttgtccatc tgcccacaac gtacggacaa attgtaaaaa ggattgcttg
     6661 aaaataaaat tttctgcatc gtcgatttca gcgatactaa taatctgtaa aatcaattca
     6721 ttcagtgata ggtcattgag aacaaaattt ttataaacaa cttcgaaatc ttttcttaac
     6781 agctccatgt tctcattggt ataatttcta ctgttgaaca aagaaaaaat ataatagaca
     6841 tcttcttctt gtaagtctag aattcctttt tgctcttcca atccttgaca gactaattga
     6901 aataacggct tcgtttctgc gcttttctta tcttcttctt tcagtccaag gggaaatgcc
     6961 tttcttccga ttgcgatctc tattcctcgt atgataaagg acttttcatc ttccgaatat
     7021 cttcttttaa aaaaatgagt ctctacataa tcaatcaact cgttacaagc ttcttctaca
     7081 tacggatttt tattttcata gccttcccaa ttgacataac gtactaaagc tagcaagatg
     7141 ttccgcgagt caaattctgg gataatgatt tctttatttt tcaaataatc attttcttta
     7201 aaaaaatcca atacgatttt tttgattgta tatactttgc tcaaagaaat aaattcaaga
     7261 tcagccagtt ctgtactcgt gaatatacca ttaaaataat aacttaaaaa ctttaaaaaa
     7321 taagattgac cgtacagaaa ctgtgtgagc tcagaaagag caataccatg tttgaatacg
     7381 agtttatact tatgcttctc tcttacttgt tcttttatac aatattctag acactcggct
     7441 agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat agctgtttgt
     7501 tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt tctaaatata
     7561 tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt cttatgattt
     7621 tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa agccctttca
     7681 aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctcacaatt tcagcctcat
     7741 tgtaggtagc tttgagccgt ttggactttt accccagcct ctttcaagaa tggttattca
     7801 aagatgtcag cgtctagttc tttgactaca tctttcaaca attggatacc agctgcgctg
     7861 ttaccaaatg gatctagtgc cgggctgaag acacctatgc cgtaacgatt tggcacagct
     7921 gccatcaacc cgccgcctac accgcttttt gcaggtacac caacatggac ggaaaactct
     7981 cctgattcat catacaaacc agcagttgtc ataattgatt tgacgattgt agcactttct
     8041 tcagtaatga tttgtttgcc attccaagga gcaattcctt tatttgccag cacagcagct
     8101 aattttgcta tgcctgtagc ggtgacattg agggagcatt gtctgaaata agcatcgagt
     8161 atctccggta cgtctccttc gatcatttga ttacctttca tatagtaagc aagagagcga
     8221 ttgatatcac ctgttcttga ttcagaatga tagatttctt cattcaatgt gatattagga
     8281 tcatcacaga tctctttcat aaatgacaag attcgctcaa atttttcatc tgcatcttta
     8341 ccggcaatca acgaggttgt tgcaatcgct cctgcattaa caaaaggatt cattggatgt
     8401 ttgcgatgat tgatttccat attcatgata gaattgaagg caaaaccggt tggttccgtg
     8461 ttgattgttt gaaagacttt ttctattccg ttgtctttga ttgctaataa caatacggga
     8521 acttttgaca tgctttcgat ggcaaagcgc acatcggcgt ctcctccctc aatcagttgt
     8581 tttgtatcca aatcataaat cgccatacct aattgtttgg gatccacttc agctaatgct
     8641 ggaatgtaat ccgccacttg tcctgtttga taatagtgga aattttttcc aatcaattgt
     8701 tctaattctt ttacttccat ttgaataacc agctttcatt ttttattttt ctgcatgctg
     8761 gaatttatga cggcatgcgt agatcgtaaa tggaataact aagacgataa gataagaaac
     8821 aatcagaatc gtttcataaa tcccatgctg tccagctgcg atactagcag gtggaacaaa
     8881 tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa caagcaacga ttacttttac
     8941 aacttttccg cctggaacct gataggctct ttcaagactg tcatctttaa aaaccagctt
     9001 caaatagccc gcgaaaaaca taaagtacaa catcaggtag attacaacag tcaatgacat
     9061 cgctgcaaga aatgaaagat tattcccccc gccgcttaat gtaaagacgg ctgcccagat
     9121 tgttgcaacg atcccatttg cgatcatcaa atgagcagga acaccgtgtg tgttttcttt
     9181 tgcaaaatat tcaggaagta atccttcttc tgctgctacg tgaagtgctt ttgaaggaga
     9241 ggttacccaa gaagcaattt ctccgatgac acctaaacaa acaagaatcg caagaatttt
     9301 cgcaatccat gcaagatttg gatcaatgtg tcttaatagg aattctaacg attgaatcac
     9361 ccctgtatta agagacaaac catgttgagg tactgtagta gcgacggtca atccgccaaa
     9421 agtatctaaa atgattgcca gaataaccaa tacaaacatt gctaatggat aatccttttt
     9481 agggttttcc atttcatttg catgactggc agatgtttct acgcccatat atgctaaaat
     9541 aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa tcaggaatca atgttttcat
     9601 agacagtatc gtttgtagtg gagcgcctga agcgacataa tggatcccta agccaaacaa
     9661 gactaatgct ggcaacaaga taccagctac aaaaccgatc cgagcaatca atgaagtgta
     9721 tttggttcct tttaactgtg agagtaaaat agcccagaaa ataatcaata ggattgccaa
     9781 tttcagatat ttattgtttt gtatagcacc aatacccgtt gcataggata atgcaccagt
     9841 taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga aaaagattgc
     9901 ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac cgcctttttc
     9961 ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa accatagcaa
    10021 accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg tgggatattc
    10081 atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg ttaatttttt
    10141 tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt tgaatttgtt
    10201 tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt
    10261 agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca gcacataggc
    10321 atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc agctgcttgg
    10381 attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc
    10441 gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct ttttacgaaa
    10501 atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg cactttttgc
    10561 ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc ctgttagtac
    10621 gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag taatcatcgc
    10681 aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag
    10741 tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc
    10801 tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt gaccaagtgt
    10861 gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa
    10921 atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc aaacagggaa
    10981 ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat tcaatctccc
    11041 tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt ggtaaagaaa
    11101 gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg taacatgtct
    11161 catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc caaaggccaa
    11221 tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa aagattgaga
    11281 catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag ctagaccagg
    11341 agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc ctgtattgat
    11401 aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa agaaggtgta
    11461 gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga cagacaatta
    11521 ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga caaaaaataa
    11581 acggtcttct ttttatagaa aaccgtttat tttcgtcttt ttttattttt aaagatattt
    11641 tttcataagg aataaagaa
//
LOCUS       DACDBG010000082        11171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDBG010000082 DACDBG010000000
VERSION     DACDBG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.082"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IVZ90_002236"
     CDS             6..305
                     /locus_tag="IVZ90_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8263260.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVZ90_002237"
     CDS             378..1739
                     /locus_tag="IVZ90_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8263261.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVZ90_002238"
     CDS             1761..2021
                     /locus_tag="IVZ90_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263262.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVZ90_002239"
     CDS             2035..2787
                     /locus_tag="IVZ90_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8263263.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVZ90_002240"
     CDS             complement(2881..3486)
                     /locus_tag="IVZ90_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8263264.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVZ90_002241"
     CDS             complement(3539..4513)
                     /locus_tag="IVZ90_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8263265.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVZ90_002242"
     CDS             complement(4891..5670)
                     /locus_tag="IVZ90_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8263266.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVZ90_002243"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVZ90_002243"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8263267.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVZ90_002244"
     CDS             7161..8558
                     /locus_tag="IVZ90_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8263268.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVZ90_002245"
     CDS             8639..9079
                     /locus_tag="IVZ90_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8263269.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVZ90_002246"
     CDS             9227..9553
                     /locus_tag="IVZ90_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8263270.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVZ90_002247"
     CDS             9554..10303
                     /locus_tag="IVZ90_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263271.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVZ90_002248"
     CDS             complement(10383..10838)
                     /locus_tag="IVZ90_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8263272.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3548 a         1956 c         2293 g         3374 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt a
//
LOCUS       DACDBG010000083        10713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDBG010000083 DACDBG010000000
VERSION     DACDBG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.083"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            241..2472
                     /gene="pflB"
                     /locus_tag="IVZ90_002249"
     CDS             241..2472
                     /gene="pflB"
                     /locus_tag="IVZ90_002249"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8263273.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2716..3477
                     /gene="pflA"
                     /locus_tag="IVZ90_002250"
     CDS             2716..3477
                     /gene="pflA"
                     /locus_tag="IVZ90_002250"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8263274.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3562..4488
                     /locus_tag="IVZ90_002251"
     CDS             3562..4488
                     /locus_tag="IVZ90_002251"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8263275.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4670..5563
                     /locus_tag="IVZ90_002252"
     CDS             4670..5563
                     /locus_tag="IVZ90_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8263276.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5697..5822
                     /locus_tag="IVZ90_002253"
     CDS             5697..5822
                     /locus_tag="IVZ90_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263277.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5956..6588)
                     /locus_tag="IVZ90_002254"
     CDS             complement(5956..6588)
                     /locus_tag="IVZ90_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263278.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6736..7278)
                     /locus_tag="IVZ90_002255"
     CDS             complement(6736..7278)
                     /locus_tag="IVZ90_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8263279.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7413..7802
                     /locus_tag="IVZ90_002256"
     CDS             7413..7802
                     /locus_tag="IVZ90_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263280.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7806..8570
                     /locus_tag="IVZ90_002257"
     CDS             7806..8570
                     /locus_tag="IVZ90_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8263281.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8699..9646
                     /locus_tag="IVZ90_002258"
     CDS             8699..9646
                     /locus_tag="IVZ90_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8263282.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9776..10516
                     /locus_tag="IVZ90_002259"
     CDS             9776..10516
                     /locus_tag="IVZ90_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263283.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         3769 a         1794 c         2124 g         3026 t
ORIGIN      
        1 ttaatacagg tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa
       61 aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg
      121 taaataaaac ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc
      181 tgtgttatac taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca
      241 atggagcaat ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac
      301 tttattcaaa ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca
      361 gaagcaacag aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt
      421 aacggtgtct atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc
      481 tatttggata aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa
      541 gcattcatgc cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat
      601 acaccaaacg atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt
      661 gtatttgacg cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg
      721 cttccagacg cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat
      781 ggaatcgact atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca
      841 atgagtgaag aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg
      901 caaatgaaag aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca
      961 aaagaagctg tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt
     1021 gctgcgatgt caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg
     1081 gataatggag taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa
     1141 ttacgtatgg tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct
     1201 atctgggcta ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag
     1261 aatgatttcc gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg
     1321 actgttttgt attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct
     1381 atcgaaagtt cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct
     1441 gatgattgtg cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc
     1501 tttggcgctc gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat
     1561 gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac
     1621 tttgaagaag tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc
     1681 aatacgttga acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg
     1741 gctttgatgg attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat
     1801 gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa
     1861 ggaatagcag ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac
     1921 cgtgcagatg aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat
     1981 aaaacttacc gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt
     2041 tacggtaaaa atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca
     2101 ggagcaaatc caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca
     2161 gctaaaatga attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct
     2221 tcaggtcttg gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga
     2281 tacttctcaa aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt
     2341 gatgcaatgg aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca
     2401 gttaaattcc gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac
     2461 gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa
     2521 tagccatcgc atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg
     2581 gcaggacttg tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa
     2641 aattttgttc agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa
     2701 aagatgtgaa cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc
     2761 ggttcagtag acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt
     2821 tgccaatttt gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca
     2881 gatgaacttt tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt
     2941 actgttagtg gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa
     3001 gcaaaagagc ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa
     3061 gaacctttct tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac
     3121 atcaaacaca tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt
     3181 ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta
     3241 gttcctgagc gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc
     3301 ttgtcaaatg tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg
     3361 gaaacacttg gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc
     3421 gaaaatgcta aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc
     3481 gcatcggaaa gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga
     3541 aatcacaaag gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat
     3601 acagatgcaa tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa
     3661 acagaagcag ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt
     3721 cagctatctg caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt
     3781 gtggatcata atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct
     3841 gtagtagacc accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca
     3901 gaaccagttg gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca
     3961 attcctaaag aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc
     4021 aaatcaccaa catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct
     4081 gaagtcgatc taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat
     4141 aaatcagctg cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac
     4201 gttcgtatcg ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag
     4261 ctagaagcag caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt
     4321 gtcacaaaca tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa
     4381 gtagaagaag ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt
     4441 tcacgtaaaa aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt
     4501 atgaagcatt caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta
     4561 aaggccgaag aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt
     4621 acgtaaaagc cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta
     4681 ttcaaaaaat aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt
     4741 gaccgattat ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga
     4801 attttcgcaa aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa
     4861 gagggaagaa cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca
     4921 acaagccact tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga
     4981 acaaaaatta gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga
     5041 agcgtatttt tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg
     5101 aaatgagaag taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc
     5161 taattacttt tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc
     5221 cgaacttatt ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga
     5281 caggatacgt aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact
     5341 tgtgacgaat actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa
     5401 aaacaatttt gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc
     5461 ctttcttaaa cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc
     5521 ttatatcaaa gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa
     5581 attatactga ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg
     5641 agtattttgc ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc
     5701 aaaaaaatgg tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt
     5761 taaataatca atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt
     5821 agattaggaa gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat
     5881 aatttatcca atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt
     5941 atgaagtcgt aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca
     6001 cataaataca cctagactga cgatgaagag catatccaac agatttcctg cgacttggat
     6061 cgtcgaaggg ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat
     6121 aaaacaggtg acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag
     6181 tgaaaaaata aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa
     6241 gaaaagcgta ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa
     6301 tgcaatcaat acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc
     6361 accgaaataa acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt
     6421 ttcttcggct tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg
     6481 cgcatgtgag gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa
     6541 aaaagagcct aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa
     6601 ttaaattgat aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt
     6661 aatcaaaata ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt
     6721 ttttaagttt gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc
     6781 tgaaatatat tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag
     6841 gcgatacagt tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc
     6901 ttccagcccc attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg
     6961 caaactttta tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa
     7021 tgccaatttg tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata
     7081 gacaaataaa ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata
     7141 aatcgtgatc tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa
     7201 catgaaacgt aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa
     7261 catttttgga tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa
     7321 aatcgtaagt gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata
     7381 atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg
     7441 ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc
     7501 caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg
     7561 tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga
     7621 atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt
     7681 gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc
     7741 agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt
     7801 aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg
     7861 ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga
     7921 caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga
     7981 tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct
     8041 atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt
     8101 tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa
     8161 tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg
     8221 gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt
     8281 atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat
     8341 tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa
     8401 aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa
     8461 aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg
     8521 aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga
     8581 aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt
     8641 taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat
     8701 gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt
     8761 catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc
     8821 tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat
     8881 catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct
     8941 gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga
     9001 tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt
     9061 cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata
     9121 tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga
     9181 aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc
     9241 aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg
     9301 gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac
     9361 agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat
     9421 tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt
     9481 tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg
     9541 tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta
     9601 tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct tattactata
     9661 tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa
     9721 caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa
     9781 agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt
     9841 aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa
     9901 tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat
     9961 ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga
    10021 aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac
    10081 aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac
    10141 agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta
    10201 ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc
    10261 tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac
    10321 agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg
    10381 aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg
    10441 tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa
    10501 tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc
    10561 tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt
    10621 tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca
    10681 aactattttc gcagatcgtt tgcaaaaata gtt
//
LOCUS       DACDBG010000084        10667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDBG010000084 DACDBG010000000
VERSION     DACDBG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.084"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(72..1217)
                     /locus_tag="IVZ90_002260"
     CDS             complement(72..1217)
                     /locus_tag="IVZ90_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8263284.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(1278..1925)
                     /locus_tag="IVZ90_002261"
     CDS             complement(1278..1925)
                     /locus_tag="IVZ90_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8263285.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            2119..2412
                     /locus_tag="IVZ90_002262"
     CDS             2119..2412
                     /locus_tag="IVZ90_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263286.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            2456..2767
                     /locus_tag="IVZ90_002263"
     CDS             2456..2767
                     /locus_tag="IVZ90_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263287.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            2764..3388
                     /locus_tag="IVZ90_002264"
                     /pseudo
     CDS             2764..3388
                     /locus_tag="IVZ90_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            3688..4050
                     /locus_tag="IVZ90_002265"
     CDS             3688..4050
                     /locus_tag="IVZ90_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263288.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            4087..4935
                     /locus_tag="IVZ90_002266"
     CDS             4087..4935
                     /locus_tag="IVZ90_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8263289.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            4925..6400
                     /locus_tag="IVZ90_002267"
     CDS             4925..6400
                     /locus_tag="IVZ90_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8263290.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            6666..7073
                     /locus_tag="IVZ90_002268"
     CDS             6666..7073
                     /locus_tag="IVZ90_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8263291.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            7076..7291
                     /locus_tag="IVZ90_002269"
     CDS             7076..7291
                     /locus_tag="IVZ90_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263292.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            7295..7675
                     /locus_tag="IVZ90_002270"
     CDS             7295..7675
                     /locus_tag="IVZ90_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8263293.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            7809..7964
                     /locus_tag="IVZ90_002271"
     CDS             7809..7964
                     /locus_tag="IVZ90_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8263294.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            8033..8506
                     /locus_tag="IVZ90_002272"
     CDS             8033..8506
                     /locus_tag="IVZ90_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8263295.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            8503..10197
                     /locus_tag="IVZ90_002273"
     CDS             8503..10197
                     /locus_tag="IVZ90_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8263296.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            10163..10348
                     /locus_tag="IVZ90_002274"
     CDS             10163..10348
                     /locus_tag="IVZ90_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263297.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            10352..>10667
                     /locus_tag="IVZ90_002275"
     CDS             10352..>10667
                     /locus_tag="IVZ90_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8263298.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFA"
BASE COUNT         3659 a         1736 c         2188 g         3084 t
ORIGIN      
        1 ctaggagaaa ccttgatatg atagggtttc tccttttttt attttttact gtatgccata
       61 ttgtatgcca tctagtcaat atttaagtgt tgtactaatt tttcaacagt cttttctttc
      121 gtttcttttg ccaagtgacc gtaacaatcg atcgtctgac taatttttgt atgccgtaat
      181 ctagtttgaa cttctaccat agtagctcca gcgtccaaga gcattgaagc atgagtgtgt
      241 cttgctacat gcacaccgag aaatggaacc atagcttttt cacaaatagt aatcaatctt
      301 gcataaatag cgtggtgtgt gattagttct ttatagatac cacaaaaaat catagtcgga
      361 tcagttacac caagtgtcag catatatttc cgctgattaa tctgccattt ttttaatata
      421 gaaattgttc tttcatctag tggaagaacc ccagcactac aatcagtttt aggatcactg
      481 attttatagc catttttgga ttggcttagt gttttattga tggtgaccgt attttcttga
      541 aaatcaatgt ctgaccaatt caatgcaagg gcttcactga tcctaacccc gctataaaag
      601 agaaagcgta gtaacgtttt atcaaagtcg tttcgataag tttcaggttt gctgtttagg
      661 taatcaaaca aggtgatcag ttgttcttta ctgtaaactt tgaccttttc tttatgctta
      721 gtggtggttc ttttaggcat taatacgttg tctagtggat tagtctcaat aatcccaaga
      781 ttgatagcgt agctaaaaat acgcttagaa gttgaatgaa gtaccttgta ttggtcagta
      841 gtttcagccc agctatttac aatttgttgg gcttttttta ccgtgacttc attcacacga
      901 agatttccat attttggaag aatccacttt ctaaacttga tttcagtttt catgaaggtg
      961 gcttctcgga cagtggtttt atatgtttct agccacaagt cataaacttc tttaaacgtt
     1021 tcgttgttcg cttttttaat accgttttta tcgtagtcca cttgcaagcg tgacaaggca
     1081 agctgggctt ctttttttgt tttgtaacct cgtctacgaa ccttatcagg tttgccagtc
     1141 attttgttca ttccaagata ggcttcatag agccaaaatt tacctttttt agtttcatat
     1201 tgcttgaatg ttgccataat agttttccct ccatagtgta gcaagccgtg ggggcggttg
     1261 gaggaggcag tttattatta ttttgaatag tgtttattta gctcagtaac aagagctgtg
     1321 aactctttta gaagatcatt ttcataaaat tcttgatact cttttttctc atctgtattt
     1381 ttttctgaat cattaattaa tgagtcatat cgtaaaagcg tatttagaag agctgcgaga
     1441 taagcaattg gggtactccc gtcaggaaat tcaattcgag atcctgtttt aagatatttt
     1501 attgcgtttg ttaaataaat cttctgtgag acagtcaggt tatcttcttc gatgttattg
     1561 atagcattag aaagattaga atcaattata gagttatttt tttcaatagt ttttttcaat
     1621 tgcttttcaa gcacatttcg cttaatttcg ggcatcatat gcatatctct taatgtataa
     1681 gatccgttta atagataggt agtggataca tctaatacct ttgaaagttg tttaagcctg
     1741 gtgttattgg gaagattata gccattttcc caattactaa cggcaccttt actggccggt
     1801 ggggataatt tacctccaag ttgttccatt gagagtccct tttcaattct agccagtcta
     1861 attctttgtc ctgtctccac tgcttcgggg tatttttttc tagccacatt ttcaccacca
     1921 ttcatatatt ttctctacta tatcacgatt taaatatgtg tgaaacataa aagttcaaaa
     1981 aagtatgtta aaattcttgc aaatgaaaag gtatgatggt atattgagtt tataagaggt
     2041 atagaaatgt attcaagagc ctcttgcatt tttttaaaat ctttgtatag aaatgtatgc
     2101 taacgagggg ggataaatat ggaattaaaa attgacaaac tcacgcttga tgatccagct
     2161 tttaaaatta ttgacgatcg agttgttcaa caagtagagc aaactcaagg aacgttactt
     2221 tcattattag aaaaaaagta ccagtttcca aaatatatga acaaggcaca agctgctaag
     2281 tatatgaacg tttcatacaa tactatgatg cagaaatatg ttcctaacgg tttaaagttg
     2341 attatagttg atggagtcgt aagaatttca caagatgaat gtgatcgctt tatggaagaa
     2401 taccaaaaat aaacgcaagc cgtgggggca aagggattaa taggaggaaa taataatgga
     2461 atcgacacaa aataaagcaa tcgaaaaagt attatcaaca attcttacag aagacacagc
     2521 ggcagaatta gcaaacttgg aaggcaaagc gctagaagaa acgtttgaat gtttgtacga
     2581 acaaatggac tatcaaaaat tgctaccaca agaaccaacg gtaagcggag tattgcgtgg
     2641 cttgaacgat ttagtacaag cagagtttaa agaacgactt tctattgaag agtatcaaga
     2701 aattttgtat cagcaagttg agttgttagc gagcttgcta gggcttgaac tggaggacgt
     2761 tgaatgaaaa ctgaaatttg gaacggacac attataagat ttgtcgatat taatgatgaa
     2821 tggtgggcag tggcgaaaga tgttgcggaa gcgttaggtc ttaaacaggt tactagagcc
     2881 attcactctt tgcctaaaga tggggttact actagtaagg tcatcgattc attgggcaga
     2941 acacaagatg taaacatcat caacgaaaaa aaatatttac cgcatggcat tcaaaagccg
     3001 taaaaaagaa gccgaggcat ttcaagactg gattttcgac atcatcaaag aactacgcca
     3061 gtccacagga cttgaaggct ttcaagtatt ccgcatgcta gacaaagaac accaaaaaga
     3121 agcaatgact aaattaagcc acgctattac tgaaccaaaa ccagtagact acattaaagc
     3181 gaatgtgata gccaataaag ctgtatcaac tatttatggt cattccaaaa tggtaaaaaa
     3241 gaaagatatg actcctgaaa tgttggttga tcgtgaacct attttagatg agacagtaga
     3301 attaatgact gttaaagaaa aatatgggtt gcagttttca gtgagtgaga aaatttataa
     3361 tcgttctgct gaattgcaga caacttagga gcattaacat gatcgagaac aaagaaaaag
     3421 ccttatccat aacagtcttg gcggacagga taaaggcaac ttaattggga taaaacccga
     3481 tattctttgt tccgattata ccatattaaa aaatcggagg gaaccattat gaaacgaaac
     3541 ttaattatag gaagcatttt aatcggcgga attttcggct tattaccatt aattgcaaaa
     3601 atagctatct ttgcaggatt agcgttatat ctcttcactg cttatgacga gtacgactat
     3661 caattaagaa ctggagggca taagtagatg tacaaaacaa aactattaaa ccaactcgat
     3721 agccttgaat tagaggaaat taatcaaggg atcgctgaac tagaaaataa tatcgggaaa
     3781 acctactttg gaaattcatt caatgaaaaa ctaacagttt tgtatgtact caaaaaacat
     3841 gcagaacaca aaataatttg tcgagaaatc aacgagctaa aaaatcagat tttaaccgca
     3901 tggttaaaca ttactgatat gcaagaagca agggtgaaaa catttaatac atgggtaaag
     3961 tatcagaatc aattaaaagg tgctgaattt gttcgggatg ggttgaaata cgaacttgag
     4021 cagttgaagc tcatggaggt gtctgaatga tttataccag tattttgaaa aaggaggaga
     4081 ggtttattgg caaataatta tcaagcagca acggaattac aaaagtcggg atggtcaatt
     4141 tatcccttat ctcctggaac taaaattcca ataaaaggct ctagtggttg gaatgatgca
     4201 actaacgatt ctaatcaaat taatgaatgg tggacggaag atgcaactag aaatattggg
     4261 ttaatgcttg aagatcatca aatggtggta gttgacctag atcaacattc tgaaacacga
     4321 aacggcgttg agaattataa aaaacttgct tcaatgtatg atccatttcc ttctacttat
     4381 acggaaatca caccaggtaa aggaatccat ttcttcttta aaattcctga tggtgtaatt
     4441 atccaacaag aaacaggtgc atttgctgat atcttcgggc gtgatgataa aggtaaaagt
     4501 ttaagcggga ttgatattat cactaaagga gttccaatag cgccaactat gattatttca
     4561 agaagtgttg ggagagcata tgaacctatt gaaaacgtat ctctaaataa tattgcacca
     4621 gctcccgatt ggctagtgaa cctactgcta aaaaaaccgc aagaaataaa gactcaatac
     4681 cgacccgtag caaaaacagg gacagcaaaa aaattagata tgattgttca aggcgctggc
     4741 gaaggtagta gaaacgatca tttaactaag ttatgcggtt ggttactatg gcacggtgta
     4801 gataatcaaa ctttagcaga attgatttat acagcaaatg cgtacaatga accaccgcta
     4861 aaagataaag aagtgaatca aattattcga tcgatgatta aaaaagattt gagggggaaa
     4921 gctcatggct cataagcata aattagatag tattttagac tttcccgaag ccagcgagcg
     4981 tgaagacaat atcattgaat tgaaaacctg gatgtcacga ttaagatgca ataaagacga
     5041 tcagattaaa agtaacagtg ttgtgaatgc agaattgatt ttaaccaatg atagcaattt
     5101 agctggaaca attgcttata acgaatttag cggatacatt cacctattaa aagattctcc
     5161 ttggatcaat cgtaccgctg gagaatggga agacagtttt gaggatgcct taacggcata
     5221 tattgaagaa aactacaatg tagtttttga tgacaacaaa atacacaaag cagtagtaaa
     5281 tgtggctaga aagaatgttt tcaacccagt aaaagaacgt atcgaaaaag ttaaatggga
     5341 tcaacagcct agactggaaa cactgtttat tgacctttta ggggtagacg acaaccttta
     5401 cacacgagaa gtaacaaaac gttggatagt cggatcagtc gcacgtatct ataaaccagg
     5461 aataaagttt gagatcgttc cagttttgga tggaccgcaa gggattggta agagtaccgt
     5521 tccagcatta ctttacacgg atgatttttt caccgattca ttagattcgc taggtgaaaa
     5581 gaaagatgat tatatgcagc ttcaaggcaa tgtaattatt gaacttggag aattatcttc
     5641 aatgaacaaa actaaaattg aacagataaa aaacttcatc agtgccaaga tcgataaaat
     5701 tagacctcct tatggtcgga atgtcatagc ttgggcaagg caatgtgtat ttattggaac
     5761 gtcgaatgat gggcagtatt tgaaagatga tacaggaaac cgccgttttt atcctttgcc
     5821 ttgtaagaat aagccaaaga tgaatccgtt taaaacaaag gatgaatatt ttcttcaagt
     5881 gttagctgaa gcaaaggtgc tttttgataa aggacaacga atctatttta gtcccgatga
     5941 agataaagaa gtcttagaaa ttgcaaaaga ctatcaagag gatgccaaga tggagaatcc
     6001 aattaaagaa gcaatcacta aatatttgga aatggaaatc ccgtataaat gggaagaagc
     6061 tccagcgtgg gcgagacgtt cttattatca gaactatcct gacagtaaat gtaatgagaa
     6121 aaatttacag catttctata atggagctac tatgcaacaa gagttttatt tagttgatag
     6181 cgtactcacc gctgatattt tggaagctgt ttttgataaa caagcaaagg atttattgaa
     6241 tggtcgatca gatgcagaaa ctaaaaagat tgccttgatt attagcagta ttcctggttg
     6301 ggaacggaag caactgccaa aaagaaataa acgacgagga ttttacaata agtcaaatgc
     6361 ctatgaaaat aaaaatcgag ctggaaagta caaaaagtaa cgggtagtaa cgggtcagta
     6421 acacctagcc cgttactagt ttacccttac tgccccaagg gatacagcaa tttggtaacg
     6481 gggtaacacc ttatttcctt aaaacttttg aaatagttag ctaatatgct ggggaataat
     6541 gttactggat tttacccgtt acctgttacc aaaacgctag aacccttgtc cccgttggga
     6601 ttgagtggta acgggtacct gttacgtagg tgttaccacg tgttacgaat caaggaggaa
     6661 agtaaatgct caatattatt tcaacaaaca aagcaccgaa ctttcaatat acagatgaaa
     6721 tggatcggtt tctaatgaac acgctggcat ttagtgtggg attggtaaca gaagattatt
     6781 ccacatttga tccagaagta ttaaagataa tggaagaaga acctgattgg ctgcaggagt
     6841 cagtagcgtg gtgtcaatcg ctagtagtgg gatcattggt agatagtggc aactatgatg
     6901 atacaggtga gctaatggat gaatttaatt gtctgcttaa cctatatgat cgagcgagac
     6961 aacgagagct tacatcgaat gaggataatt tgtttttaaa catccatgat aagttcttgg
     7021 ctttgctact aacagatgat gaattgataa ctaatttatt ggaggtagaa taattatgca
     7081 tatggaaagt attagatact tcgatggtga gaaacacatt gagtattcaa acactcaaga
     7141 agatgtagac tttattagtt ttgaggaaga taaaatcact aaagttaatt ttaaagacgg
     7201 aacgtgcttg aagatagttt cgccttatat tgaatacaaa tcaaaatggg aacaagatga
     7261 ttctgacgaa cgagatcgct ttggttggta atctatggca gttaaaaaac aatgtaatca
     7321 tgctggatgt aaaacattga ttgattatag gcagaagtat tgtgggaagc ataaagcaaa
     7381 gcaaacggca attaagcgtg aagaaagaaa gcaaagcgaa ggtaaatatt ttcaattcta
     7441 tcaaagtaga acatggcgaa aggcttcata cttgtatcgg ttaaatcatc cagtatgtga
     7501 ggattgccta gaagaaggct tgataaggaa agctgatgtt gtagatcaca aaattgaact
     7561 aaaagatgat tggaatagac ggttagatga aactaatttt cgttctttat gccatgcaca
     7621 ccataattcc aagacagcca atgaaagaca aagacgagaa aagagtaccc tttgagttaa
     7681 gggggcatag tgtgaaactt actgacaatc gatgcctact cattttggta caaataaccg
     7741 ttgtgaaaag gcataagggt aattacaaat aacgattaca cttgtaatca caagtgaaaa
     7801 agtatataat gaaagtagga gatttattga aaaatacaat tgttattact gataaaacag
     7861 ggaaacagcg caccattgag ttacctcatt ttggtcaagt gaccattcaa atgcagaacg
     7921 gaaaaatcat ttatatagat aaattagata aagaaaaatt ctgatcgaaa aacgaaggaa
     7981 tgtgagctta attgcttacg ttcctttttc ttttgtctga aaggaggaca acatgggaca
     8041 ccctaaaata ttacacgata caaaaggcaa tatatcaagt gaagaaaaag caatccgtgt
     8101 ggacgctaga gaagaattgt tcaagcagca accattaata aatatcacgc cccctgactg
     8161 gatggcagcg agcgctagaa gtgaatggaa tcgtatcgta ccaacattaa aaaaagatta
     8221 tccattgagt gaagcagatt atggttcatt ggtagcctat tgtttagcct ttgctcgaat
     8281 gaaaacagcc gaagccgaga taagaaaatc aggtacgttt atcacatgcg aaaacggagt
     8341 aaagaaagcc aatccagcgg ttcgggttca atcacaagct atgagtgatt tgaaaaaaca
     8401 agccacctca ctaggtatga ccttagaatc acgatcaaaa ctagctttga acaaggttaa
     8461 aaatgatgaa cccgaagacc cgttcaaaga gttgatgaat tcatgaatga ttacattgag
     8521 aaagtcctat caggtaagct gattgcaccc aagaaaatta tccaagcgtg tgagcgtcat
     8581 ataagcgatt tggagcgttc taagtcagat agcttccctt atgtgttcga tgaagaacaa
     8641 gccaccaaag cgattaaatt cattgagttg ctaccatcta cggacggtaa agcaatcaag
     8701 atgttaggat ttcaaaaatt cattctaggc agtctttatg gctggcgtac taaagaaggg
     8761 aattacaggc gattcaatcg agcgtttacc agtatgagcc gtaagaatgg gaaaacgtat
     8821 atcgcaagtg gcatggctgc caatgcgttg attatggaac aagaaccagc agaagcaagg
     8881 caagtattgt ttgtaagtaa cgctttgaaa caagctaaat tgggctatga tatgctgtct
     8941 aattcactta gaaacgtggt caagtctagt aagtttttaa gaccacaact gaaaattatg
     9001 aactctaaga ttcagcactt gccctctaat tcgttcgcta tggcactggc tagtgaaacc
     9061 agcacgctag atgggtttgc accaacaacc gcaattcttg acgagtggca cgaagcaaaa
     9121 actcgtaaaa cgtacaacgt cattaagtca ggaatgaccc aacaaaagaa cggcttattg
     9181 tgtgttatta gtaccgctgg gcttgattta aacgttccta tgtacgagga atacttattg
     9241 ttagatcgtg tgctaaaagg cgaagaacaa gccgacaggt actttatagc gatatgggaa
     9301 ttggacgatc ccgaagaaat tcacgatcaa gagaaatgga tcaaagccaa tccgattttt
     9361 gaaagtgaag aaatcaaaaa agtaatgatt ccaaccattc aagatgatgt gaaccttgct
     9421 ttgaaacaaa ataaccttaa ttctgtattg gtgaaaaact tcaatttatg gagacaagcg
     9481 agtgaggaca gctatatgat tgctgaagac tggcaggcaa ccgaagtaga accacaagat
     9541 attacaggca agcccgttta tatcggagtg gatttatcta aaacagatga tttaactagc
     9601 gtttcatgga tcgtaccgct agataacggc gaactttatt gtgattcaca tagctttgta
     9661 gccaccaaat atgggcttca agacaaagaa aagcgcgacg gtttgcctta tcgagaacta
     9721 gaaaaagctg gtgagtgttc cattactcaa ttagaaagtg gaatcgtgga ctatgaccaa
     9781 gtatttcagt ttattcaaga tttgattcaa gaaaatgatt tggaatgtat ggggatttgt
     9841 tatgacccgt ataacgctaa ttcgcttatc agtaaagctg aaaaagccaa ctacccaatg
     9901 ttagaagtaa gacaaggaac gattactcta aacgttccga cccgaacttt tagagaacaa
     9961 gtttatgaag gcaacgttat tcacaataaa aatacgattc tcacccatgc agtgaacaac
    10021 gctattttaa aaacggataa caacggcatt cagattaata aatcaaagaa cagtaacaaa
    10081 attgatccaa tagctgcatt aatcaatgcc tatgtgtttg caatggatta cttcaccaca
    10141 acggaaggag cgaaagcaga caatgaattt tatacaagtg aagaattttc tttctaatta
    10201 cattcatacc gttcttttac ttcttggatt ggtgtgtgtg ttggttgcta tcaccttact
    10261 aacaaatgtc tactatggct tgttagcgct gggcatagtg cttattgggg tagcggtcat
    10321 gctaaataca gaacagaagg gaggttaaaa gatggcattt tttaaagcga gacaaaatac
    10381 aacgggagat cctttcctac aacatgtggt atcaatccaa tcggatgatt acaccaccag
    10441 ctttacaagc gttcgtgcat taagaaatag tgatgtgttt gcagccgttc gaattattgc
    10501 tagtgatatt gcttcaagtc cgattcaatt ggtcaaaaac aatatgccac aagctgataa
    10561 tgaactggtg aagttactaa acgagaaacc taattcagaa atggatggtt ggcatttaaa
    10621 gtttgctttg gcggtcaata tgttgctaaa cggtaatagc tttgcag
//
LOCUS       DACDBG010000085        10665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDBG010000085 DACDBG010000000
VERSION     DACDBG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.085"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..787
                     /locus_tag="IVZ90_002276"
     CDS             200..787
                     /locus_tag="IVZ90_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263299.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            787..1686
                     /locus_tag="IVZ90_002277"
     CDS             787..1686
                     /locus_tag="IVZ90_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8263300.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNEQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1686..2600
                     /locus_tag="IVZ90_002278"
     CDS             1686..2600
                     /locus_tag="IVZ90_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263301.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQMLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            2652..2867
                     /locus_tag="IVZ90_002279"
     CDS             2652..2867
                     /locus_tag="IVZ90_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263302.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            3058..3312
                     /locus_tag="IVZ90_002280"
     CDS             3058..3312
                     /locus_tag="IVZ90_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP8263303.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            3491..4540
                     /locus_tag="IVZ90_002281"
     CDS             3491..4540
                     /locus_tag="IVZ90_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8263304.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            4717..4947
                     /locus_tag="IVZ90_002282"
     CDS             4717..4947
                     /locus_tag="IVZ90_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263305.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            4950..5207
                     /locus_tag="IVZ90_002283"
     CDS             4950..5207
                     /locus_tag="IVZ90_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263306.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            5327..6298
                     /locus_tag="IVZ90_002284"
     CDS             5327..6298
                     /locus_tag="IVZ90_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263307.1"
                     /translation="MKTKGFNVKENAIGGNKIFKKDIYVWFNGKKYMTTNKRWSELVQ
                     NTVKKVMPDGYHFNYIQCSQTTESIYFSILNENRLYYFRVSSHHRKKREDNVTTFLSG
                     KYNGFKELSEAIGEYLKSNRFVRISDKHILILLLYLNNSFKLFWRDDTFYYIENGMDI
                     YSGINGILKHYIKALLRYGVLYQEDLSNQIKISNSGKSLLAKYKKLLSPIYKGIDMEA
                     VNSETLFNLVVDEKFANEFFKSKKIERLNNLFPENAIKAYVDNNKSRLYNWFIPKRLR
                     GKIKIGDQVKVINLNHTKIVTVTELYIAEKEDITKMKPVIKILDNKE"
     gene            6308..7102
                     /locus_tag="IVZ90_002285"
     CDS             6308..7102
                     /locus_tag="IVZ90_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263308.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            7131..8249
                     /locus_tag="IVZ90_002286"
     CDS             7131..8249
                     /locus_tag="IVZ90_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263309.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            8282..8602
                     /locus_tag="IVZ90_002287"
     CDS             8282..8602
                     /locus_tag="IVZ90_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263310.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            8857..9750
                     /locus_tag="IVZ90_002288"
     CDS             8857..9750
                     /locus_tag="IVZ90_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263311.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            9770..>10665
                     /locus_tag="IVZ90_002289"
     CDS             9770..>10665
                     /locus_tag="IVZ90_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263312.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLADNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFV"
BASE COUNT         4081 a         1343 c         2257 g         2984 t
ORIGIN      
        1 tcatcttaag agaagaatta cgattgttta taggatttac aaaatgaata agtttgaaga
       61 gtgatttggt cgcagactag atcactcttt ttgtgtaaaa aaactttaca cttatttttg
      121 agtgtgttaa gattttttat atagagtgac ggtttcttgt cagagtgaat gataaccatc
      181 aatattggag gaataataag tggaaaacaa taatgatatt agatatttta gcattgggga
      241 aagagttaca acagtaagtg aattatcaaa ggattataga gataaagaag gaacgattat
      301 ttctcttaaa aagaatcgaa gaggtgtttg gcaatatgtt gttgatttag gggatggcgt
      361 tacagttgag tgtaaagata gtcaactgat cagaaatgat ggcagtcatt ccattgaaaa
      421 agcaattctt gtattcccaa aagtatcaag caagattatg aatgactggt tgcttggaaa
      481 atttggtgag tacaataaaa aagaaatcgc agaagtgatc ggcaaatctt atccaacagt
      541 attagggtat ctaaaaaaga taacgaacaa agaagcgaaa gttgaggatg ttttaacagt
      601 aagagaactt cagtcattaa tcgaatatcg gaataaacaa ataacaaatc ctgagtacct
      661 taaaaaagaa gatggtctat tgaaacaatt ggaacaggta aataaaatcc ggaagatgca
      721 aaaggtagct cctttaagcc tagataactt taaaaaaatc tatttgaaag agatcgaaaa
      781 agactaatgt ggagtgtcac tttaccgttt caacctgtat ctgcaccaag accaagtgca
      841 agaaggaatg ttgctgatgc aactaatgac gtagcagaga aaaggatttc aacctattat
      901 gataaaaagt atctcgatta tttgaaaaaa ataaaaagct ttatcgaaga tcaaggtttg
      961 ttagatgatg aattttactc gatcgtaaat gatccgatgg gtgcaattat ggaagtggac
     1021 ttttactatc agataccaaa aagctataaa aaagtctata acattatgaa gacgacagcg
     1081 ccagatttag ataatttagt aaaaagtgct atggatggta ttttcaacat ctcagctata
     1141 cgagatagcc atatcacagg gttaattgct tttaaataca atgtggcaaa cgaaggaaaa
     1201 acagttgtga ggatcaagcg atattcagaa tttgagtggg atacttctga aggattgaac
     1261 ggagatattt gggaagccac atttgatttc aaaccagtag caacgccaag accaaagtct
     1321 aaatttagag gaaatgggat tatcacttac tatccaaaag agtataacga ctatttggaa
     1381 aatatcaaga atacgcttaa agagaaagat ttggtaaatg aacaactgaa aaaggtaatg
     1441 aatgctccta tgggggttat agctcagata gattattttt accaagtgag aaaagataag
     1501 aaaaagcttg attcactgat gcgtacttca cagccggata ttgataactt agtcaaagga
     1561 acattggacg gaatttttaa caaggaaatt ggcatcaaag atagcagggt ggtaggttta
     1621 atagctttta aatacaatac ttttgaaaag tcgaaaacaa acgtgcgact acaggagatg
     1681 ggctgatgaa taataacgaa aaaattcttc atgtacttga tagctttgaa acaattcaag
     1741 aggaactaaa gaaatatcga gatgttttgg aacaaagata tgattttgtc aatcagcaaa
     1801 agagtaatca catggatttc attctcaata tgaatgactt gaagaagaaa ttagttgaac
     1861 gaaaagaaca agaaaagttg atcaaagctt actttgaact cggtgaaaaa gaagttaaaa
     1921 aggcaatgga actgaatgaa gatcgcaggg ttttagatca gatgttagaa caattattgg
     1981 tgatgtttca aaaaggacga atagatgagg atttaattga agaagggttg agaaagtatc
     2041 ctgcaaactc aggtatcgga atagtgctga aagcgattga tgaagaagaa attgaagatt
     2101 tcataccggc agaggatttt gaatcggcaa tggaatatat aaaatattac agccaaggga
     2161 tcactgcttt tagagagttt gatcctgaag atgttattca tgacttgaac aacctaaaag
     2221 aatggtgtga aggttatgaa gttgatgatt ccggtttgga ctacttgatc agtattatgg
     2281 aaatcgaaga agagatgccg gataagcctg atccaacaga tattttagaa ttaatccatg
     2341 atgctagaaa tccaattgca tacatttcta gaggatatac tgtattggaa tactataagc
     2401 cttatatatc agcgatgaac catttaagac gtgtattaag ggaaaaacgt gaataccgct
     2461 cagtgttaaa tgcatcgaat agattagaaa aagctgtttc agagctagat gcttattatc
     2521 gtgaacatta cctgcaagca ggtggtatgc caagaaatac gaaagcaaat atcagtaggt
     2581 atataaaaga agctgagtag gcggagttag cccataaaat ggcaaaaggt aacctgaaag
     2641 gggcggtttg aatgaaaagg gatgtttttg aattgtacgt attagaacgt ctagaagacg
     2701 gcatgatggc acaagagata attgatgaaa ttgatgatat gatcagcaga tttggtcaat
     2761 tggaattgaa tgatggaaac aaaaattcgg tagtgctaca acaattttgg ttaaataatt
     2821 tttcgtataa ggattttatc aaagaaaagg caaatagatt gtactgacta aattcacgag
     2881 gatcagtgat gagagcttat tgctaagagc agttaggctc ttttttgttt gtgttaaatt
     2941 attttatttt acaatacaca tttatacaca aaaaaatgtt gacggatagt acacaaatgt
     3001 ggtaggatat atttgtaagc aagttagatt aacaagttaa aggaaggaag agcagttatg
     3061 aaaaaagaag aactaataga tatgtttcaa attgttgaac gagcaaataa tatgggaatt
     3121 atgttttttg atcgtatttc tttaaagatg gatttaagcg ttgcccacca agaatttaat
     3181 ctacgattaa aagctttatt gatttctgat gatgttaatt tcgctcatga tgttgtaggt
     3241 atacagaacc atatagatag agaaaacaaa cgtatgggtg atggattctt accaagatat
     3301 tcaagtttat gatttggcga aagattcaag agtatgtgtc attattgata ggtactcttg
     3361 aattggaaag gaggaatgaa tttaagacaa caacgattat gatcttaaaa caatcgttta
     3421 tgtaattggc aatcttacga ataattttca tgcaataatg acaataaaaa atgaaaaggg
     3481 gtaaaatcaa atgaaaaaat ttttagtagg aattctattc acaatggggc tagtgctatt
     3541 atcaccttca gtttcagcag aaagctctat ttccatgtat cgtatgtata atcccaatag
     3601 cggagagcat ctatacacta gaagcgttgg agaaagagac aatctgaaac gttatggatg
     3661 gcattacgaa ggtgtcgcgt ggcaagctcc aacaagtggt tccccagttt atcgcttgta
     3721 caacagatat aacggtgaac atttttacac cgttgatgcc ggagaaagag ataagatttc
     3781 gcacacagga tggacgtacg aaggtgttgc gttctattcc tacaacggag caaatagagt
     3841 tcctgtgaca cgcttgtaca acaaatacgt gaattggcac cattacacgt tagatcaaaa
     3901 tgaaaagaac gtaattagta aacaaggttg gaagattgaa ggtacgggtt ggtatgcaat
     3961 gccaaacagc gcaccaaatc ctacacctgc acctgctcca agtaatgaaa aagtgttaaa
     4021 tgttcctgtc atttggcaag gtaatacgat gttgtgtgaa ggtgcttccc tgttagaggg
     4081 attgcactac aaaggagtag cgacaaatca aaacttaacc agttttgtaa actatatgcc
     4141 aagagctaat gacaataatc catatcatgg ctattctggc gaatggcgct acaacgtaaa
     4201 tggtacgtat caaggcatga tgcctgatcc agtagttaaa tgggcaaaaa atgttggtgg
     4261 taatgccgtt aatatcactg gttcaggaac gaatgggatc aagaatgaaa ttcgcaatgg
     4321 taatccggtt gtaacttgga ttacttataa ctatgcttca cctcaattca aacaaatgcc
     4381 ttggggacgt gcagtctgga atggacatgt ggttctcgtg gatggcttca aaaatggtgc
     4441 ataccatgtt gttgaccctg tatttggcgc aaaatggatc aatgcaagca cgtatgagag
     4501 agcatttaat gtgactggaa tggcggttgc tgtacgttaa ttgatagaat taataaataa
     4561 atgaaataag agcttaatta ctgaaatggt aaatgggctc ttatttttta gaaaaaaact
     4621 gttttttgta atacgaatct ataacaaaaa acgttgaatg tagtacacaa aagtggtata
     4681 ttaaaaccgt agagattatg cgaagaggtg attttgatga accaaagaag aagaaaaagg
     4741 ctagaagaaa taagcgagcg actatccgag ttaaaggatg agttagaagt aattcaagaa
     4801 gaagaggaag agtacaaaga aaatatacct gaaaatctac agggatcaga aagatatgaa
     4861 aaagcagaag aatccgttga tcaacttgaa gaagctgttg atggtttatc cgacatatta
     4921 gaattgattg aagatatagc tagctagaga tgattaatga agatttcagg agtgagcaaa
     4981 aaatgattga tatttcactg atagagaagc tacttttttc aaataagtac acagccaaag
     5041 agttggctac aagatggcaa atatccgaat cagctattaa agcctatcgt caaaataagg
     5101 gaagcgtagg atggcgtgat tggaaaggaa tcagcattgg aaaagcagaa gacataatga
     5161 aacgtgaagg atttcaagct gacaatgatt ctaaaaaagt tgaatagttt aatagtcgcc
     5221 aagaaaagta ccaggacgag gggaaaggta gattacaaag gaaaacactt ttaaataaac
     5281 ttcgcaaaag catcaggtta tgagtggcta gggctatacg aatgtgatga aaacaaaggg
     5341 ctttaatgtc aaagaaaacg caataggggg gaataaaatt tttaaaaaag atatttatgt
     5401 ttggtttaat ggaaaaaaat atatgactac taacaaaagg tggtcagagc tagtacaaaa
     5461 tacagttaaa aaagtcatgc cagatggata tcatttcaac tatatacaat gctcacaaac
     5521 aactgaaagt atttattttt caatattaaa tgagaataga ctctattatt tcagggtaag
     5581 ttcacaccat aggaaaaaga gagaagataa tgttacgaca tttttatcgg gaaaatacaa
     5641 tggcttcaaa gagttaagtg aagccattgg tgagtatcta aaaagtaata gatttgtcag
     5701 aatttctgac aaacatatat tgatcttgtt actttatttg aataatagct tcaaattgtt
     5761 ttggagagac gacactttct actatattga gaacggcatg gatatttatt ctggtatcaa
     5821 cggcatatta aaacattata ttaaggcgtt acttcgatat ggggttcttt atcaagaaga
     5881 tttaagtaac caaattaaga ttagtaattc tggaaagtca ttactggcaa aatataaaaa
     5941 attgctttct cctatctaca aaggaataga tatggaagct gtaaattctg aaactttatt
     6001 taatttagtt gttgatgaaa agtttgcgaa tgaatttttt aaaagtaaaa aaattgaaag
     6061 actaaataac ctgtttcctg aaaatgcaat aaaagcttat gtagataata ataaatcacg
     6121 attatacaac tggtttattc ccaaacggtt gcgaggaaaa atcaaaattg gcgatcaagt
     6181 gaaagtaatt aatctaaatc atactaagat cgttaccgta acggagttgt atatagctga
     6241 aaaagaagat attacaaaaa tgaaaccagt tattaaaata ctagataaca aagagtgagt
     6301 ggaatatatg gtagcaaaaa agaatactct aaaagaagct tctgaaaaat atcaagatat
     6361 gaaaagacga ggattaatac caaaggacaa aaaaggtata tggagagaat ttgttaagga
     6421 agtcagtagt gatcgaagtg gcggtgactt tgttggacga tatacagcaa aaggttatag
     6481 caggcgctta aggatcactg ttgaatatgg aacgaatgaa tcagctacag agctgaagca
     6541 aaggtactta gatacacgaa gagaagcttc aaaaaaatat gatgatttca agagcagagg
     6601 tctaattcct aagaagaaaa aggggatttg gcgtgagttt ttcaaagaag aagatagcaa
     6661 tcggaataag ggagacttca ttggacgata cacagcaaaa ggctacacta agaatttagg
     6721 aatcaaggtt gaatacggaa ctggtgaaag ttcatgggaa ttgagaaatc gctatttaaa
     6781 tacattgtta gagaaatata aagcagaatt attggagttg ttcgatgtta atgattttat
     6841 cgagatggct gaaaagtatg gtcatgaaga aggttttatg atcaatgaca ttatggttat
     6901 tgataatttt acggatttat tagcacttgc gaaaaaagca agggatttaa gagaaacgca
     6961 gatgtatcta ttcagaaata aaaacggaag aaaccggaga actgaatcga gtgagattga
     7021 agattactac gatgccttta cacacgatct gggagccttt gtagacgaga taagtggtgt
     7081 aatgaacaaa tatagggtct agaataaaaa agccggagat gagcagataa atgggaaaca
     7141 atagcatata cacacctgaa atgcagaata aaattaaaag acttgtcgga ttagggtgga
     7201 tgacaagagg aaaattagat tcttttagta cgatcaaata tcttttgatg gacatttacc
     7261 gtcagaacaa gcaaacagga atacattttt tgggctatga taagccggaa gatgcacctt
     7321 tctttgttcc aagttcagcc tatagagcca gtacctccgg tagcatgtat aacttggatt
     7381 tgtttgagga aggagcaaag atgatcgaat atgatttcaa cgaagcctat tcgaggatca
     7441 tgagaaatta tcctttgccg tcaaacactt atttgaaagc aaaatataat acagatcaag
     7501 ctattttgag agcgataaat agaccaaaag taagaccgta tgaaaattta aaaacgtata
     7561 tgttcttttt ggttaatttt gaaggaacag ccaaagaagg aacatatgct aatccaaaat
     7621 cacagtcgat gtttgcggat tatggcgaaa atcccaaaac agctaatgag cctatttggt
     7681 tatcggagat tgagctaaca cttatccttg attattatga tattaaagag atggaagtcc
     7741 tagatagcca cacatttttg acgaaaaaag gattactcaa agaatatttt gaaagaatcg
     7801 agccattaaa aactgatcct gatacgttga agctttataa aaatcttaga acgaaagtat
     7861 ttggtgctat cggacaaaaa tcattaagag aaggagaaac aagcaccttc gataaataca
     7921 gtatgtataa tcgagcgtat tcctccatgg tagcaggcgt atttagagat cgtatggtta
     7981 gatatgaaca gaaatacgtt catagcgcat atgggctatt aaaaattcgt actgatggat
     8041 tgtatttcag atccgaagta ccagagtttg aagagctgta taaacttgga gtagttaaga
     8101 aaaaagttca tacaatatcc aaagaagaag tcgaaaaagc tttagaattt aaaaacaaag
     8161 gatttgaaaa taagggctcc tttgggaaat ctaaagatga agttaagggt gttgtgatag
     8221 acgatgattt cgagtttgat tttgactaaa tactctttgg aaaagatggt aggaggtaaa
     8281 tatgaagaag aaaattgtgg aaggaaatgt aggtgttatt attctaaaag attcaactga
     8341 ctatgctaaa tcaatggcac taaaagacga aaaagttgca cgcatggata aagatttagt
     8401 tgaatttctg atggatgata cagaaagatt aaaaggggat cagtttttga gtttaatgaa
     8461 agacaaaggc ttaaacacat atggtttatt tgattataag aaattagcta tagagtgggt
     8521 aaaagaagga gaaatatttt ttattgaaga atgggaaggt actgagaact tagttcttca
     8581 tcagtatctt ttgaaagctt aatctaagta aaacagttct gactagttat gcttgtcaga
     8641 atttcaaagg aagttttcca taaccatcat cattcaaaaa taaaggattt gaaaataagg
     8701 gttcctttgg gaaatctaaa gatgaagtta agggtgttgt gatagacgat gatttcaagt
     8761 ttgattttga ctagattttt ttgcaatgtt taagagtaaa caaggtaaga actaagatca
     8821 tgaagattga gagtaaagaa gatagaggac gaatgtatga gaacacacta tatttattta
     8881 gaagataaaa ttgtagatga aattccggca aaaagtcgag aacaagctag aaaattattt
     8941 attgaaaaat acaaagagat caatggagat agtgaagttc caaaagtggt cgtaacagtt
     9001 agaaaaaggc aaacaaaaaa gagaagtacg tttcatgtca gccctgatat atataatctt
     9061 atagagcgac atagaagtga agataatatt tctatctctc aatatattgc tacagttatt
     9121 gatgaattga tttcaggaaa gtatgactat gcttttgatt ttgaggatga aaaggattac
     9181 aaaagtgttg ctgtctttca atctgatttt gagaagctaa aaaactatgc tggcaggaga
     9241 aatgttaatt ttgcaagact actagaagag ttctactccg aagagttggg agtaggaaat
     9301 gaatacaaga gagaaactta tgcgaaattg tctcataagt tatcccaagc gcttaaagaa
     9361 gtgacagaag ttaaagcgga actagatgct aaacttgaag aagttaaaaa atatcaaaca
     9421 gagaggaaga acaacatgaa gaatcaagtg gaatttgacg aacagaaaga atttgaaaaa
     9481 gcaagaaaac gctataacaa aattatgaat gaacttagct atgaacactt aacagttcat
     9541 gaagaattga gtgagggtac agaaaaatat acgatcgcag atatggttaa tgaagtggaa
     9601 actcttttag cttctttctt tgaaaatggt cacgtaaacg atgaatggcg aaaagaagaa
     9661 cctaaaaggg ttaacgctta tattgctcga ttaaaaaggt ttattgataa atataaggag
     9721 aatagtgaca aattttctaa agataaataa ggagagagtg tgattacatt tgccagaaat
     9781 cgaaggaata ttttcgccaa aaataggtaa aaaacagtta gatttcagca gttttccgag
     9841 cgtaaataga gattataatc taggtgatga ggtacagata ctaaatattg atttcaaaga
     9901 actagacttc gctgttgctg tttccaacaa acccaaggca ttggaacgag tgaaaaaaat
     9961 ataccaagag aatgatcaaa atatcaaaaa tatgatcaaa aagtatagac gagataattc
    10021 aaaagttgaa gacgttttat tcaacggtac ggcaaaacat gatttgaatg tttatatggc
    10081 attaggagtg gctttgtact gtaattcaat taatcagaca gatattttgg ttaatatcgt
    10141 gaggaatagc tatcaaaaaa tattaaaaat aagtagcgaa acaaaccctt attatgcaat
    10201 gaaatatctt gcggataatt tattaaatga taaaaaattg atgcacaaca ctgaagagct
    10261 gttatctaat ttggctgcga ttaactttct attcaaaaca ggttctgaag attattttat
    10321 agataacgta ctaaaaggtg cttacggaac agcattttat ccgctttaca atatgaaaca
    10381 gcataaagcc gaacaaaagg ataacaaact tgaaattaaa aagatgaaag aactattagg
    10441 tataggattt gtaaagccca attcaaaagt cactattgct tcgttagcaa ctactaacaa
    10501 tgatgggaaa atagtaaatg ggtttgacga ttcagatata gatatattgt caaaatcata
    10561 tttttctttg tatggagatt ttgcagattt gatgggtatt gatagttact tagtcaataa
    10621 ttttgagatc aagggtagcg agctgaatga tattgtgtat ttcgt
//
LOCUS       DACDBG010000086        10594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDBG010000086 DACDBG010000000
VERSION     DACDBG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.086"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..814)
                     /locus_tag="IVZ90_002290"
     CDS             complement(<1..814)
                     /locus_tag="IVZ90_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8263313.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISL"
     gene            960..1763
                     /locus_tag="IVZ90_002291"
     CDS             960..1763
                     /locus_tag="IVZ90_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8263314.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1941..2888
                     /locus_tag="IVZ90_002292"
     CDS             1941..2888
                     /locus_tag="IVZ90_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8263315.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2956..3249)
                     /locus_tag="IVZ90_002293"
     CDS             complement(2956..3249)
                     /locus_tag="IVZ90_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8263316.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3277..3942)
                     /locus_tag="IVZ90_002294"
     CDS             complement(3277..3942)
                     /locus_tag="IVZ90_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8263317.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4082..4714)
                     /locus_tag="IVZ90_002295"
     CDS             complement(4082..4714)
                     /locus_tag="IVZ90_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8263318.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4721..5788)
                     /locus_tag="IVZ90_002296"
     CDS             complement(4721..5788)
                     /locus_tag="IVZ90_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8263319.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5785..6516)
                     /locus_tag="IVZ90_002297"
     CDS             complement(5785..6516)
                     /locus_tag="IVZ90_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8263320.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6685..7164)
                     /gene="greA"
                     /locus_tag="IVZ90_002298"
     CDS             complement(6685..7164)
                     /gene="greA"
                     /locus_tag="IVZ90_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8263321.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7300..8475)
                     /gene="mltG"
                     /locus_tag="IVZ90_002299"
     CDS             complement(7300..8475)
                     /gene="mltG"
                     /locus_tag="IVZ90_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8263322.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8679..9848
                     /locus_tag="IVZ90_002300"
     CDS             8679..9848
                     /locus_tag="IVZ90_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8263323.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(10115..>10594)
                     /locus_tag="IVZ90_002301"
     CDS             complement(10115..>10594)
                     /locus_tag="IVZ90_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263324.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         3265 a         2105 c         1638 g         3586 t
ORIGIN      
        1 ataaagaaat tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat
       61 caatatgtcc gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa
      121 catcctttac cccatcgatt gcttcaattt cttgttccat tttttcatag tcgatagcag
      181 gtgcactttg tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata
      241 tatatatcga aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga
      301 tcaaaattgc tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat
      361 aactagattt tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca
      421 aattcgcgag caatccaata actgctactg ttagcatcaa atgactattt atcttttctg
      481 gtgaagtgaa ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga
      541 atgagttgat gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat
      601 ttgcttcttt tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac
      661 taaggttatg gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa
      721 attctgataa agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc
      781 tctgtggctg tgtaggcact attccttcct ccattttatt tatattggat agtctttaga
      841 caagaataac atttttttga tatttcacca atcattcagc tgtttcctta aagcacttgt
      901 atttcacttg atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta
      961 tgacaacaaa acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga
     1021 gaaatgtccc attttggaaa gccattgtct atcttttagt tttgagtata attatggctt
     1081 tgccaatctc ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga
     1141 ttgctactcg cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag
     1201 aaggattcat ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa
     1261 cagaacagga catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa
     1321 acaaacttat catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc
     1381 aattagaact tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt
     1441 cttttttaag tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta
     1501 tttatcctag ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc
     1561 tctatgcacg ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct
     1621 attcaatttc attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg
     1681 acactagcgc cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat
     1741 gggcaaaaat atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac
     1801 ccttttagag gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct
     1861 ttctcaaaaa acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa
     1921 agaaaggcgg gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg
     1981 atgaatttat cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac
     2041 aaccagagga tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa
     2101 aactattagc ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc
     2161 taccccttga gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca
     2221 gtgaatcgat tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc
     2281 aagcacaaaa ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta
     2341 tccaagggaa aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa
     2401 aatgcaaagc attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag
     2461 gaatggaaaa gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg
     2521 tagtcaatat cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg
     2581 aacactttga tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac
     2641 aagatctggt gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg
     2701 aggaagagcc tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt
     2761 cccacattgc aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca
     2821 atctgaaaag ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag
     2881 gatattaata gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg
     2941 tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc
     3001 ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa
     3061 aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc
     3121 aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc
     3181 gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt
     3241 taatttcatc ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag
     3301 aatatccaca tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc
     3361 atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa
     3421 gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga
     3481 gactcggatt tctgctaaag aatatttatc tgacaactct aggtaatcta aaatattctt
     3541 tgacactaaa ttatgcgcaa tccttattcc catgtctcgc tcgggatgga cgacgcgatc
     3601 agctccgatc ttttcaagta ctctggcatg atactcattt tgggcttttg ctaaaatatt
     3661 tggtacgccc atttccttta ccatcagagt aactaagata ctggcttgaa tatcttctcc
     3721 aatagcgaca attacatgat cgaaatttct tatgcctaat gatcgaagtg tcatttcatc
     3781 ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga cccgatcttc
     3841 attactatca atagccagta cttcttgacc agcttcgatc agtgttcggc agatacttcc
     3901 gccaaatcgt ccaagaccta caattgcaaa attttgtttc accgttttcc tcctttagta
     3961 tcttcttttc tcagtataac aaagccttta caaaaacaaa agcaagcatc attattgtaa
     4021 aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt
     4081 tttatttcac caaaccatgt ttgaatgcat agatagcggc ttgtgtacgg tcttccactt
     4141 ctagcttagc caatatattg gaaacatgtg tttttactgt tttcagtgta ataaacaatt
     4201 cgtccgctat ttcctgatta ctttttcctt cagaaatcag cattaaaatt tcattttccc
     4261 gattcgttag ttcttcatgt aatacaggct catttcgccg actcattttt tccatcattt
     4321 ttgttgtcac ttctggttct aatactcgct cgccacgttg cgttgccctt atagcatcag
     4381 caatttcatg tgcggtagag gtcttcaaca aataccctgc tgcacctgct tcaatggcag
     4441 gataaacttt ttcgtcatca ataaaactgg ttacgataat gattttagct tctggccaat
     4501 cttttaagat tgcttttgtt gattcgatac catccatttc ttccatgact aagtccatta
     4561 aaatgacgtc cggtcgaagt gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt
     4621 ctccaattac ttcaatgtct tcttgaatag ataaataaga agagacgcct aagcgcacca
     4681 tttcatggtc atctactaat aaaactttta tcatccttat ctatgcctcc ttcatcaaag
     4741 ggaccttgat ttctacactt gtaccttgac ctttaaaact aatgattttt actgttccac
     4801 caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt ttcgtttcat
     4861 tcatattaaa ccctgttccg tcgtcaataa tacgtaacaa aagattattg tctattttgt
     4921 gcaaatatac ctctaattca ttggctttag catgtcttaa tgtatttgat aacaattctt
     4981 gaacgattcg gaacaagtga tcctcaatgg aactagatag ttttacatct tctacatccc
     5041 atttcagcga aatctggatt ttattttgca attccttcaa taactgctcg ataccttgct
     5101 ttaaactttt ctcttctaaa ttgactggac gtagatgaag aagcagcgca cgcatctcag
     5161 actgggaagc gttgatgatc tctgctacca ttttcaactg cttgcgaaac atttctggtg
     5221 tctcgctttt ttctgcttgc tccgtcaatg ccgacatcat catcattgct gcaaacaatt
     5281 gttgcgaaac tgaatcatgc agctcacggg ctaatcgatg tctttctaat tctaaaattt
     5341 cttctttggt ttgtccatct acatattggg gacggctcgt tacaatttgt aattcactag
     5401 atacttcaat cattttttct tttatcttag tgatttcctt atcaatatct ttgatgtata
     5461 aatcgtccga tgcacttggg atcggaagaa gcaacaattt ggattcataa ttgccagaag
     5521 aaagtgcacg cagcttttct tcgattttcc cgtattttgt cttttgaata agtgataata
     5581 atagaaagac aattaatcct acacctaagg aaatggctaa caaatggaag ataagcggaa
     5641 catataataa acgtgcacgc agtaattctc cccaccaatg actttgattg ctggcataaa
     5701 aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta
     5761 gcatcgctct ggatattttt cccatcatat tcgtatcacc tcgacatctc caatcaaggt
     5821 attagtaata atcttcaatc tacgagggtt ttcatcataa tcactgctgt agattttgat
     5881 ttgttcattt ttcaaagaaa attgctcttc ttcaaacaag acatttccta ctaatgtcgc
     5941 atgctctaat cggatggcta cgcccaaagg tactaagatc cgtgttctgc ctatcccttt
     6001 tcttacaatc acaatattat catcttttgg caaaagcgta ttgcccaaat ctatgatagt
     6061 atcaccagaa atgacggcga tgttgatatc gtcccattca tagacgtcgt ttccaatacg
     6121 ttggttaccg aataattgct gtcttttccg ctcattatta tgtgttttta attgctctgt
     6181 ttggatcatc atgatctgtt tctttcgcca aaaagcattt ttggttaaat ctatccctga
     6241 aatctctaca cctttcagac caataaataa aacggcaaat acagccatca tccataaagc
     6301 aggactgttg actaagctaa aaaaaatgac caaacttcct aatatcaatt gaaaattttg
     6361 aaatttagta cgaggatgtt tgcgcatagc tagatatata ttgaaaatac cgaatatcac
     6421 taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc
     6481 ttcaactacc acaaaaaaac gccaagaact attcatcgct ctttctccct cctgacacat
     6541 tctcatttca tcatacctat ttttttaact gttggctata ggactttaga tgtaagatcg
     6601 tttgttgaaa tctaaaatac acaaagagaa aaagatctag agtgtatcat cagtaagatg
     6661 aactctctag atctttcagt cagactacgc ttgttctact ttgacgattt taactaacat
     6721 atcgccacct ggagtagcaa tagttacttc atcattttta cgtttgccaa tcaatgcttg
     6781 cgcaatagga gaatcattgg aaattttgcc agaaaacgga tctgcttcag cgcttccgac
     6841 aatcgtgtac tcttcttctt ctccatctgg tagttcgatg aatgtaactg ttttaccaat
     6901 tgatacctca tctgcatcta caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt
     6961 ttcaattgtt gtgattcttc cttcaacaaa tgcttgttca tctttcgctg attcatattc
     7021 agagttttcg gaaagatctc cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc
     7081 gcgtttgact gtttttagtt cttctaattc tttttctaat ttttctttac cttctagagt
     7141 cattggataa actttctcta ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa
     7201 aggtaatctt ttgaggaata tagtccaaaa aagattacca cgcttttctt caaatgtaaa
     7261 tgtttttcta aaaatgatca atgtctagag cctgtttttc taagaattat ttacatattt
     7321 ttccactaat tccatatgtt cttcataagt tttagcgaaa taaacatttc cagtgctcgt
     7381 atctgctacg aaataataat agtcattttc tgctggttct aatactgcct tgattgcctc
     7441 ttcgcttgga ttgtcaaaag gtccaggtcc atagccggta ttggtataca aattatatgg
     7501 tgaatctaca gctgtatctt catacgtcac taattctttg tgttcaccta atgcgtataa
     7561 aacagagata tctgactgaa gtggcatatt tgctttgatt cggttaaaga agacttgtgc
     7621 aatgttttta cgatcatttt cttttacgcc ttctttttca actagagaag ctagagttaa
     7681 aacttcttgt acagtcaaat ttttctgctt gatagctgaa taatagtttt gcatgaccgt
     7741 attcgtcttg ttcaccattt caatcactag atccttaagc gaagtactcg tgtaataatc
     7801 atatgtggct gggaataagt aaccttccaa acggtatttt gtgttctcag ctttagatgc
     7861 actttccaac agttctggga atttttggtg caactcgtta aagaaagtct catcattcac
     7921 taaatctaaa aattctttct tgtctttacc agttaccttc gctacccgtt cagcaatctg
     7981 atcgatatcg tacccttctg gaatggcaat tttcccatct gcaacttttg ttggctcaga
     8041 agtaccgcct tcttgcaact gtttgccaat ctcgtcaaga gtcatattag gcgctaactg
     8101 ataatagcca gcttgaaagc cagttaaatt tttaaatttt gtataataat tgaaaacaat
     8161 cccgctttta ataatattgt ccttctccaa aatctcacca atctgcttgt tagaagagcc
     8221 actcgggatc tcgacttgga ctaattggtc atctgattta tctaaaggct tcaacccgct
     8281 atctacataa cgatacacgg taaatccaag aaatccgcca ataatcaata atgtaagagc
     8341 aatcactaaa atgatcttcc tcacaatctt atcttctttt ttacgcagat ttctccgctt
     8401 gtcgattata tctgctgtag ctgccttact ggcttctttt ttatctttac ttgggtgttg
     8461 tttgtttcct gacaaaacaa ctcctcctta ctatcaagtt cgtaacttta ttcattatac
     8521 atgatatttg aattggcgtg aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt
     8581 aaatccatta ctataagatt aaaataaatg ttatcaagat acaagctatt ttagctttat
     8641 tctttctgta aaaaagaaag gagactaaat aaactattat gaaaaaagga aaacatgcta
     8701 aaaaaagaaa atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct
     8761 ctacaggaac tattcttgga tggcaatttt acaataatga acactctcag atagttgccc
     8821 atgctcaatc agctcaaaaa aagttgatac tgactaccaa tgaacaacag ctcgttgaaa
     8881 aaatcgatca actattaaga aaaaatgatt atattggctc aatttacgta aagaaaaata
     8941 atcgcatcat catggaaaaa ggctatggct atgcgaataa actcacgcaa caaccaaatg
     9001 atccttctct ttattatcag attggttcgg ttcaaaaagc attgactact cttttgattt
     9061 taaaacaagt cgagcgtggg cacctttctt tagatactaa gctggctcaa ttctatccac
     9121 aaattgctgg cagccagcaa attactatcc gtgacttgct gtatatgcgc tcaggcttgc
     9181 accgaactgc ttctccaacc ataccgatga ctgatgaaga agtcgttcaa tttgcgattc
     9241 agcacttaaa atttacagat tatcagacct ctcattatga ttcattgaac tttaccatat
     9301 tgactggaat tttgatcaaa ttaacaaatc aaccgtatga aaaattattg accgatgata
     9361 tcatcgaacc tttaggatta cagcatacaa aattttatga ccaagtcaaa aattcatcca
     9421 atcatgcact atcctatcaa atgtctaaag aggatgatta ctttaaacca ctgacagaat
     9481 ctgaaacaga gatccataac gaattaggga ctggtaatat cagtatgtcc gtctatgatt
     9541 taaatacgct gtttacacaa gttttatctg gaaaactagt ttccaaagac ttactcttct
     9601 cattatggcg tgaaaatgcg aatggacatc catatagtgg tggggtatat agcggagata
     9661 attatatcct gtcgcaagga aatatcgact cttttgatac atctgctata tttagcaaag
     9721 attcacagga tgctatcatc atggaaagca atataaaagc tgacaaaact atcaaactgc
     9781 ctactgctga tctaagaaac caaatctatg acctgatgga aggaactact aagttggcaa
     9841 atagctgata tgatgaaaag atagcaaata ctacttcttt gaatagcatt tgcttaaaaa
     9901 aagtactcga ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa tttttgctac
     9961 agactttaat tccaaataaa cagaacagaa agaggtgttc ttctttctgt tctgtccaaa
    10021 ttattctaag atttctcttt accaacatta tttgacaaag ggcctattaa atctaaaata
    10081 gtatctttag attgctaaaa tatgtgctag caaatcattc atggcttgtt cttattttta
    10141 attttatgca ataaaaaaga agaacaatta gcgtgattat agaaataaat aatgagattt
    10201 tagaaaaaaa tgtgtatgaa tacctgattg ttactttatt tattccactt tcacttttta
    10261 ctgtaacaaa aggacctcct tgttttagta ctgtagcatt ttttgaatta acagtaaccg
    10321 ttaatccttt ataccggaat acaggtaaaa ctattgtttt gccttttgaa gatttaaacg
    10381 taaatgtaat ttcatcttta tttggagaaa agctactata aataacttga ccatcttctg
    10441 taacttgatg gtttttcaaa agcaattgat cttttttata gcctataggt acataatcaa
    10501 aattctcaaa ttcttcaagt ttattttcca tttgttcatt agaataagtt ccttgaaaat
    10561 tcatttcttt caccatgtta actactgatg aata
//
LOCUS       DACDBG010000087        10492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDBG010000087 DACDBG010000000
VERSION     DACDBG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.087"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IVZ90_002302"
     CDS             complement(<1..357)
                     /locus_tag="IVZ90_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8263325.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IVZ90_002303"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IVZ90_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8263326.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IVZ90_002304"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IVZ90_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8263327.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IVZ90_002305"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IVZ90_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8263328.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2237..2869
                     /locus_tag="IVZ90_002306"
     CDS             2237..2869
                     /locus_tag="IVZ90_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8263329.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="IVZ90_002307"
     CDS             complement(2871..3776)
                     /locus_tag="IVZ90_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8263330.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4514)
                     /locus_tag="IVZ90_002308"
     CDS             complement(3861..4514)
                     /locus_tag="IVZ90_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8263331.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="IVZ90_002309"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="IVZ90_002309"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8263332.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="IVZ90_002310"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="IVZ90_002310"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8263333.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="IVZ90_002311"
     CDS             6763..7848
                     /locus_tag="IVZ90_002311"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8263334.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="IVZ90_002312"
     CDS             7845..8909
                     /locus_tag="IVZ90_002312"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8263335.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="IVZ90_002313"
     CDS             complement(9158..9961)
                     /locus_tag="IVZ90_002313"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8263336.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IVZ90_002314"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IVZ90_002314"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10492)
                     /locus_tag="IVZ90_002315"
     CDS             complement(10268..>10492)
                     /locus_tag="IVZ90_002315"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP8263337.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3103 a         2220 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aa
//
LOCUS       DACDBG010000088        10084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDBG010000088 DACDBG010000000
VERSION     DACDBG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.088"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(245..757)
                     /locus_tag="IVZ90_002316"
     CDS             complement(245..757)
                     /locus_tag="IVZ90_002316"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8263338.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(771..3068)
                     /gene="recJ"
                     /locus_tag="IVZ90_002317"
     CDS             complement(771..3068)
                     /gene="recJ"
                     /locus_tag="IVZ90_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8263339.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3210..3644)
                     /locus_tag="IVZ90_002318"
     CDS             complement(3210..3644)
                     /locus_tag="IVZ90_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8263340.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3654..4442)
                     /locus_tag="IVZ90_002319"
     CDS             complement(3654..4442)
                     /locus_tag="IVZ90_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8263341.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4439..5380)
                     /locus_tag="IVZ90_002320"
     CDS             complement(4439..5380)
                     /locus_tag="IVZ90_002320"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8263342.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5557..6144)
                     /locus_tag="IVZ90_002321"
     CDS             complement(5557..6144)
                     /locus_tag="IVZ90_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8263343.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6365..7678)
                     /gene="obgE"
                     /locus_tag="IVZ90_002322"
     CDS             complement(6365..7678)
                     /gene="obgE"
                     /locus_tag="IVZ90_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8263344.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7966..8976
                     /gene="gap"
                     /locus_tag="IVZ90_002323"
     CDS             7966..8976
                     /gene="gap"
                     /locus_tag="IVZ90_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8263345.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9061..9996)
                     /locus_tag="IVZ90_002324"
     CDS             complement(9061..9996)
                     /locus_tag="IVZ90_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8263346.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3262 t
ORIGIN      
        1 ttacgggggt gtgacaaaaa tcggtcacat cctttttttc gaataaacgg tgttctaaag
       61 ccagtttttc ggtattaagt caaattgcgg aaaaagtgat tttttaaact tctactggtc
      121 tttgcttgtt tccatttata aaaaaatagc gaccctcaaa agtcagatag agaatctgac
      181 ttttgagggt cgctttaaaa tcgcttcttt aagaataaca tcaagaatag cttctttttt
      241 ctgcttaata ttccattaat gtaatgatgt catatccttt gattttgtcg cgtccatgga
      301 gatccatcaa ttcgatcaaa aatgcgcagc ctacgactac accgcctaat tgttcgatca
      361 gttcgatcgt cgctttgatt gttcctccag tagctagcaa atcgtcacaa atcagcacac
      421 gttgacctgg tttaatggca tccttatgca atgtcaatgt atctgttcca tattctaatc
      481 cataagtcac ttcgatggtt tctcgaggta acttcccttt tttgcggact ggggcaaaac
      541 cgactcctaa ctcataagca acaggacatc ctacgatgaa tccacgggct tctggcccaa
      601 ctaccatatc aattcgtttt tcttttgcat aatttacgat ttgtttagta gcttcccgat
      661 aagcgtcacc atctgccatc agtggggaaa tatctcgaaa cacaattcct tttgacggat
      721 agtcgggaat actggcaata taatctttta aatccactct ttgtttcctc ctaggttgtt
      781 aaccatttct ttatggtagg aatatcgctc aaaagcaaaa attcctcaac tttgatttta
      841 ttgagccgtc gctggtataa tctgctctca gtcaaaggat gactttctgg gctagcgact
      901 ttttgtaaaa caccgttctc tattgtaaca aatttcaatt caaaaaacac ctgtatcata
      961 aaaattaata atttttcagg gatacgcaaa tattgagcta ttgcttttat tttatgacgt
     1021 atatcaattc gttcttgaga atggatcaat ttaaacaatt ttgcgtactg ttctcttgtg
     1081 cctaccccat tcaaataggc ctcatcaggt gaaatcccca tcagataaac acgattgaat
     1141 gaataagacc gaaaaacttc tttcagatcc gctaaatcat ctggacaatc cataacaact
     1201 agagagtttg gttttctttt ttcaattatt gcctttacag aatctagtcc ttcgaaaaca
     1261 atcgcagctt cctggataat ctcaggcacg aatttcaacg tcgaagaatc aaatgctaaa
     1321 taaaggctac cttccgacgt tcggctctgt tcacggaaac gttttgcccg ccaatcaaat
     1381 acttgcagac cacccacaga aaaatcagat accatcagct gcggtttctt acgcccgttc
     1441 cattcattga tcgaaagctt tcctaccaca tctacctgat tattcagcag ttcttcttct
     1501 tgagtaccaa aaccaaaagc gaccgcatca agctgactcg atgcatcgga aaggacaaat
     1561 ttcagatgct gttggtttgc tccgattctt ttgatatttt ctgcttggac ttgtcgaaat
     1621 aaaaactgag gcaaaggatt atccgtacca aatggcgcca atagtttcag ctcatcaatt
     1681 cgctctactg ttacttcatt tggcagcaat acttcatcaa ttctcaactc aggccctctc
     1741 atcaagtcaa tttgatgatc gactatatat tggttcatct tctcctgtag tatagtgaca
     1801 ttttcacttg gcatcgttaa accgactgct gcatgatgtc ctccgaagta agtaaataaa
     1861 tctcgcatct ggtcaagcat ttcaaaaaga ttcaaggctt caatactacg tccagatcct
     1921 ttagctgatc catcttcttt taaagtaagg acaagcgttg gtttacccgt ttcattcatt
     1981 attttcccag caacaatgcc caaaacgcct tcatgccatc ctcggtttac caatagatgg
     2041 atatgattct ccttattgac catcgctaat gcttcttgtg ttatctgctc tacgattgct
     2101 tttctttctt cattgatctc attcaatttt tttgcttgaa cggatgcttc ctcttcatca
     2161 aacgtcgcaa gtaacgagac ggcaggacgt ggatcgccca tccgaccgat tgcatttagt
     2221 ctgggagcaa tcgaaaaacc gatcgttgtt tcgtctgcgt ccctcatttt tacgccgctt
     2281 tcttcaaata gcgcctgaag tcctatccgt tctgaatgat gaatggcatc gattcctaat
     2341 gaaacaagga ttcgattctc atcagtcaat gaaactaaat cggcgatcgt tcctatcgct
     2401 actaaatcca aaaattctgc aggcggttct tccaataatg cagaagctac tttgaatgct
     2461 actccgactc ctgccaaatc tggaaatgga tagtgccccg ctggatgacg agggtgaaca
     2521 atcgcataag cgtctggtaa attctcaggt agttcgtgat gatctgtaat gatcacgtct
     2581 acaccagctt gctgagcgta tgctaccgca tcatttcctg ctaccccgtt atcaacagtg
     2641 atgatcaact ggattccttc ttcgatcttt tcttggtata ccgattggtt tggtccatac
     2701 ccatgctcaa atcgatttgg caggaatact tctacttctg caccaagtaa ttccagtgtt
     2761 tctttcatta ccgttgcact ggtgatccca tctgcgtcat aatcaccgta aacgaggatt
     2821 ttttctccac tgattacagc ttcttggatt cggctgattg ctttatccat atcgtgtatc
     2881 agatacggat catgtaattc ttgttccttt ggatgtaaaa atcggttgat atccttttcc
     2941 tcatggtaac ctctttgcca cagtaactta ccgacaaatg gagataattt cagttcacgg
     3001 atcatttgga gaaaggattc agaaggctct gtctcgctta acttccaatc aaaatttgct
     3061 tgtctcactt tctcactcct aacttagtta ttatagcaaa ggaaatcagc aaaaaaaagc
     3121 agaaagaaag aataaaaaca gatcagatcc gctagacaga tgttcagcag gacctgatct
     3181 tttgtacgac catttatgaa atatccctat tagtcaaaat aattgaagct ttgtttgggc
     3241 gattgagttt tttgttcttc cggcgctttc aattgactga tttgtgcggt tttcgcttgt
     3301 aactccgcct gataagcagc ttccaactcc gcacgttcgt ttctaaactc gcgttgctgt
     3361 tcttcttttg cttcttcaat tttcttttga agttctgttt gatattctga catttcctga
     3421 gtcagctgtt tgatttgctt tttttgctgc catagtgttg ccgaagcagt caaaaataca
     3481 atcagtgcac caatcagcgc agaaccgatg atcactaaga tcaatggcgc agaaaaggaa
     3541 gtgaagccaa aacttacagg aacattttta ttgttcaata cagcaaaaag aacaataatc
     3601 aaaactaaaa caaatccaag aatcacattg ctttgctttt tcaattcctt cacctacttt
     3661 ttgttaaaaa ttccgctggc taaataatca ccaatcgttg gaaacagcgt ataaaatttt
     3721 gatgcaactt ctaaaataaa cggttgattg atttcccgtt ttggatgtct catattgcgg
     3781 acgatcattt ttgccaactt ggcaggttct aaaacgattt gtccgacttt ttccaaatag
     3841 cttccgcttg gatcagcctt atcgaagaaa tttgtctcga ttggacctgg attcactgtc
     3901 gtaacagcta cgccaagtgg tttcaattct aatcgcaaag cgttggagaa gccaaggact
     3961 gcaaatttcg tggcagaata aaccgttgat tttgcagttg ccatttttcc agccatagag
     4021 gcgatattaa tgatatgacc ttgtcttctt tctgccatat cgatcgcaaa tttttgtgtc
     4081 aaaaccatca ttcctaagat gttgacttca aacatatcgt atgcttttcc aaggtcgaat
     4141 gtaaggaagt cttcgaaaat cccaaaaccc gcattgttta ccagaatatc gacgcgcccg
     4201 acttcttcca tcaccttttc atataattcg tccacgcttt caggattgct gacgtctacc
     4261 ttgaaagcat aagcctcttt gccgctcaag cgtctgcact cgtctctgac accttctaca
     4321 aatgccattc ttcgtgcgca acttataacg attgctcctt gctttgctgc ttcataacag
     4381 atttgtgcgc ccagaccgcc tgaactacct gttactaaaa caactttatc ttctaaattc
     4441 atgcctcttc ctcctcgcga aatggaatct caatgctatc aaaatctttc ataatcgtcg
     4501 tgttttcaaa cacttcttgt gcttcttttt caagctctaa agctgctttt cctaaatatc
     4561 tcgcactgat atgagtaagt agcagccgtt ttgcctgcgc ttcttttgca acttcagcag
     4621 cttgatgagt cgtggaatgg aaatagttgt gcgccattct tgcttcatct ttattaaatg
     4681 tgctttcatg taccagaata tcgctatttt ccgcaagcac aacggaatta tgtgtttttc
     4741 tcgtatcgcc taaaatagtc acgattcgtc cttttttatc tggacctacg aaatcttttc
     4801 cattgatcgt ccgcccatct tctagttgga tcgtttcacc ttgcttcagt ttgccgtaca
     4861 gtggtccagc tggaatacct aattctttta gacgatccac ctgcaattct cctttatgat
     4921 catgttcaac cacacgataa ccaaaactgt cgatcccatg atttagtttt tttgtataaa
     4981 cagaaaattg ctgatcagta aaaatcgggg ctgtttcatt tagctcaatg aatttcagcg
     5041 gataacttaa acgcgtttgc gaaataccta aagagacttt tatgtattcc tctatccctt
     5101 ttggcccgta aatctctaat ggcgtatccc caccttgaaa tgaccggctg ctgatgagcc
     5161 cgggaagtcc aaaaatatga tcgccgtgta aatgtgtaat gaaaatttta ccgatttttc
     5221 tcggacgaat agtcgttcgc aaaatctgca tttgcgtgcc ttcgccacag tcgaacaacc
     5281 aaacttcatt gcgctcgtct aaaagtttca atgctatact tgtcacattt ctatgttttg
     5341 caggaacacc tgcacctgtt cctaaaaatt gtaattccat aatctacctg actttcttta
     5401 agttcctctt taattttagg gtaaaaagaa gggataatca accgtaagtc gagaatgggt
     5461 tcaaattaaa aataacgttc taaagcgata gcgttttctt tgaaaattac tataaaaaca
     5521 gcagcgaacc cgtttgagat cctctgctgt tttttattaa tttgaagatc cagaactttc
     5581 attgtcgttg gaagtttgcg gatgtttgtc cttccaagtc ttttgtgcag cttctttcgc
     5641 atgattttca gcttctttcg catcatgcac atctttttcg acatcttcat aatccaagcg
     5701 agtaatttct ccgcctaatt ccatcattac caattgattc agtgggcgat tgtcctcttc
     5761 atcagcaatc aagatcagtc ctgtagctcc ttcaccgatt ttatttccta caaagtcgaa
     5821 cacggtctgg gcatctttta tttctttggc atcttgtgtc gcaccgatca ttcctccggc
     5881 aaaccatcct aataaaatcc ctagcggacc acctaataca ccaaccaaca ttccgattaa
     5941 tccacccgta gaagatttgt tattaccggt aaagtcgata aaatcttcta ttttgaattg
     6001 atgtgtccca ttaggatcat ggctgacaac tgccatctgt tctcctttaa tcgaacgaac
     6061 agcatgtaac tttttgattt ctgaaaatgc ctgatatgcc tggctttcta cttgaaaatt
     6121 taaaatgatt actcgtttgc tcattaaaaa cgcccctttc ataccttctt gttatttatt
     6181 ttacctttaa ttgaaaaata agaaaagaaa agcactcttt ttttccaata aaatagggct
     6241 gtgacaaaag tcgtatcaca gctccttttt ccgaataaac ggcgttcttg aagctgatca
     6301 cttctgtcac aacctccctt aaatctgaat ctatattttt tggtatttgt ttttatttgc
     6361 ttgcctattc gacaaattca aattcaaact caccgatacg tacaaggtcg ccatctttcg
     6421 caccgcgtgc ccgaagagct tcatccacac ccataccacg taattgacga gcaaaacgca
     6481 tcactgtttc atcatgatcg aaattcgtca tttggaataa tttttcgatc ttctcaccag
     6541 ataagatcca cgttgcatct gaatcacgat cgatttggaa ctcaggacct tctggattga
     6601 atccatagtg tacagtttct tcttccagtt cttcttcgta caatgggaat tctggcgtta
     6661 cttctaacaa atctgctgta gcattcaata acgcgtccaa tccttgtcga gtcactccag
     6721 aaattggaaa tactgggatg tcatcagcaa attcatcttc tttttccttg ttcagctgtt
     6781 ctttgaattt cactagattt tccggtgcat ccggcatatc cattttgttt gccacgatga
     6841 tttgcggacg ctctagcaag cgtaagttat aagtagataa ttctttgttg atggcaagat
     6901 agtcttcata aggatctcgt ccttccatcc cactcatatc gatcacatgt aaaattactc
     6961 gtgttctttc gatatgacga aggaattgtg tacccagccc aacaccttgc gaagctcctt
     7021 caatcaagcc tggcaagtct gctgcggcaa aacttctgcc atcacttgta gtaaccattc
     7081 ctagattagg tacgagcgtc gtgaaatgat aagcgccgat ttttggacga gcagatgaaa
     7141 taacggatag taaagtcgat ttcccgacag atggaaaacc tacaagtcca acgtctgcca
     7201 atactttcaa ttctagttcg atttttcgct cttgtcctgg ttcaccgttt tcagcaattt
     7261 ccggtgcagg attccgagga gaggcaaaac ggatattccc tcgtccaccg cgtccacctt
     7321 ttgccacaac tagtgtttga ccattttcaa ttaaatctcc taaaagagca ccagtctccg
     7381 catctcggac agtggttcct tgtggtactt taacataggt atcttctgaa ccacgaccgt
     7441 gcatcccttt actcatccca ttttcacctg gctgcgcttt gaaatggcga ttgaaccgaa
     7501 aatccatcaa tgtacgtaac ccttcatcga cgatcaagat gacatcgccg ccacgaccac
     7561 cgtctccgcc agccggtccg ccgtcaggaa cgtatttttc gcgacgaaat gcaaccattc
     7621 cgtctccgcc cttgccagct ttcacatcga tcgttacttg atctaaaaac atggacataa
     7681 ttaagtcctc ctattattca tttatttatt tgactgcctg aatttccaca ataaacaaaa
     7741 gtcttactta ttttatcggt gaaatctgat tttgtctatc atttcagcaa aaagattgtt
     7801 taccttcttc tttttttatc atttggtaag gtttccaacc ttttttctgc taccaagtat
     7861 aattttttga caaaatattc tttgcgtagc agaataacag tgtattaatt gtattaattg
     7921 aggatgccgt ttcacagcac ccctaataaa ggaggaactt ttgatatgac agtaaaagta
     7981 ggtattaacg gatttgggcg tattggccgt ctggctttcc gccgcatcaa agaagtctca
     8041 gatgatattg aagtagtagc aatcaacgac ttaacaagtc caactatgtt agcacaatta
     8101 ttgcaatttg actctactca tggtacttat cctggcacag ttactgcaac agaagactca
     8161 atcgtagtgg atggcgaagc aactcgtgtt tatgcagaac ccgacgcaag caaaatccct
     8221 tgggcaaaag aaaacggtgt agatatcgta ctagaatgta ctggttttta tacatctgaa
     8281 gaaaaagcaa aagcgcattt agatgctggt gtcaaacgag tagttatttc tgccccagct
     8341 ggtgctatga aaaccatcgt ctataacgtc aatgatgata cattagatga aaacgacaaa
     8401 atcatctcag caggttcatg tacaacaaac tgtttagcac ctatggctta tttcttaaat
     8461 aatgaatttg ggatcgaagt tggtacaatg acaacagttc atgcctacac ttccacacaa
     8521 atgctcttag atggacctgt aaaaggcggt aatttgcgtg ctgctcgttc agctgctgac
     8581 aatactatcc cacattctac tggtgctgcg aaagcaatcg gcttagttat ccctgagcta
     8641 caaggaaaat tacaaggaca cgcacaacgt gttccagtcg ttgatggttc attaactgaa
     8701 ttggtttcag tattgaaaac aaaagtaact gctgatcaag taaacgaagc aatcaaaaaa
     8761 cacacagttg acaatccttc attcgggtat gatgaccgtc aaatcgtttc tagcgatgtc
     8821 attggaacaa ctcaaggttc gatctttgac ccaacgcaaa cagaagtaac aactgctggt
     8881 gatttccaac ttgttaaaac tgttgcatgg tatgacaatg agtatggatt tacttgccaa
     8941 atgattcgct tactagaaaa attcgctaac ttgtaatttg atagatcaat cgataaaaga
     9001 agcctttgac tggatccagt caaaggcttc tttcttttca tcccctactt ttaaactcgt
     9061 tcaatgaaca tattggctta aaaattgttg gactacttgc tgatattttt caggatctgt
     9121 cttgtatgct tccgcatgct cggctccttt tacaacatac ttttcttttg gtcccttcgt
     9181 tgcacgatag accttatcta acatttcaaa tggtacaaat gtatcttcgt ctccatgtat
     9241 aaagagcatt gggcgctggt ttttcttcaa ttgttccact gcatttgctt caccgaaaaa
     9301 gtagccagca cggacttttg tcactagact cgttacttgc ataagcggaa atgctggaag
     9361 actaaacata tcgtctagct gataagcaag ctcatcatga acggaagaat aaccgcaatc
     9421 ttcaatgatt gctttcacat tatctggtag tttttctccg ctagtcatca taactgttgc
     9481 tgctcccatg ctgacaccgt atagaacgat ttcttgcgat tttccattgt tttctagcac
     9541 tttgtttatc cactgtacat agtctttacg ctctggccat ccaaatccaa tgtaatcccc
     9601 ttcgcttttt ccatgtcctc ttgcatcagg aacaaggaca ttgtaaccca tatcatgaaa
     9661 catctttgcg taattggtca tcgtttcagc gtttcccata tagccatgag ccacaagagc
     9721 tgttttttca cttttcgttt cagcaggaag ataaatcgct gataatttca gaccgtcttt
     9781 tgaagtgatg gtccaatcgc ttcggttttc ttttgaacta aaccatttat gtgcatccgt
     9841 ttctttggat gtctgtgttg taccaggtgt atcttcgtct aaaaagtctt tctcagaagg
     9901 cacaacagca taattataaa aataattccc tgcaaacact aacgcaacaa tcagagctaa
     9961 agcgacgcct aaaaggattt tcatccaaag tttcataaat catccatctc atttctcttt
    10021 tttcatataa ccttgaatta gtataatcaa agaatctcta ctatgcaatg ttcattttta
    10081 taac
//
LOCUS       DACDBG010000089         9968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDBG010000089 DACDBG010000000
VERSION     DACDBG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.089"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(190..2310)
                     /locus_tag="IVZ90_002325"
     CDS             complement(190..2310)
                     /locus_tag="IVZ90_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8263347.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2540..3718
                     /locus_tag="IVZ90_002326"
     CDS             2540..3718
                     /locus_tag="IVZ90_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8263348.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3734..4492
                     /gene="aroD"
                     /locus_tag="IVZ90_002327"
     CDS             3734..4492
                     /gene="aroD"
                     /locus_tag="IVZ90_002327"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8263349.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4515..5000
                     /gene="ybaK"
                     /locus_tag="IVZ90_002328"
     CDS             4515..5000
                     /gene="ybaK"
                     /locus_tag="IVZ90_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8263350.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5071..6324)
                     /locus_tag="IVZ90_002329"
     CDS             complement(5071..6324)
                     /locus_tag="IVZ90_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8263351.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6442..7791
                     /gene="gdhA"
                     /locus_tag="IVZ90_002330"
     CDS             6442..7791
                     /gene="gdhA"
                     /locus_tag="IVZ90_002330"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8263352.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7903..9249
                     /locus_tag="IVZ90_002331"
     CDS             7903..9249
                     /locus_tag="IVZ90_002331"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8263353.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9557..9943)
                     /locus_tag="IVZ90_002332"
     CDS             complement(9557..9943)
                     /locus_tag="IVZ90_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8263354.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3189 a         1802 c         2042 g         2935 t
ORIGIN      
        1 atatgaataa agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt
       61 gtaaaaagca agaggttgtg aagaagctgc cctattctat taccgtagat gcaagctttt
      121 gaacactgtt tattcgaaaa gaggagcagc gacaataatt ttgtcgctgc tccttttaaa
      181 tttattttgt cattcggaag aacttgtggt cgttccgtga agttcttggt acgtttgcgt
      241 tttataaaga tcaacagttg attgtccgtt gtgttcacta aagacactag ttgacttgtc
      301 accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt cataatcact
      361 ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac cattattgat
      421 ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa tttcattctg
      481 tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga tcgtaccacc
      541 gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa gctgttcatg
      601 atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta cacctagtaa
      661 gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga ttccgccctg
      721 tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat tgtcgtagtt
      781 tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc ttgaattcga
      841 gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc cgctttcttt
      901 caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg caaagtaacc
      961 gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat tggtgaattt
     1021 tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt gttgcagacg
     1081 ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt attggaacga
     1141 attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc ctaaattctt
     1201 gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg cactagtgta
     1261 accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc cgcctaattg
     1321 tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg tttccgcatc
     1381 gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg tgctgttgct
     1441 gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg agaccattct catctgtcaa
     1501 tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca cattttttcc
     1561 tttagcgagc ccgaacaaat cgtcatttgg tgctgcataa tgttctttta cataattttc
     1621 tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct ggtaagtttg
     1681 aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat aatcacgaga
     1741 gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat ttcctgagaa
     1801 aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg ggcgagggtc
     1861 caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga tccaatagat
     1921 cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg ctactttccc
     1981 agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat aagtgacatt
     2041 cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat aagcaactct
     2101 agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa tagggttgat
     2161 aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa ctaaataggc
     2221 aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga gcctcttatt
     2281 caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac ttcttaatca
     2341 cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt tactagtatg
     2401 acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt ttctttaagc
     2461 ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt gggaagagaa
     2521 agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca atcgattgaa
     2581 aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag aaaccacgca
     2641 atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc taggaaagca
     2701 gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc aatctttttt
     2761 tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag atgattctac
     2821 taccgctttt cgtatattca atggagaagg agacggaatc ggcggtctga ttgttgatta
     2881 ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac atcgtgaatt
     2941 attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg agaaaattcg
     3001 atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg caccggaacc
     3061 cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag gattaatgac
     3121 tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt tcgcttctgg
     3181 gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg ccgcaatggg
     3241 aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa aaacacagga
     3301 acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta tggacgtatt
     3361 cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg tattggatcc
     3421 accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt atggggaact
     3481 ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt ctacaaatgc
     3541 tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc atgccaaaaa
     3601 tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga cgacggatac
     3661 ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata accattagaa
     3721 ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta gaccaaagat
     3781 ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag ttcttgcacg
     3841 tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag ttttttcttt
     3901 tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac tgccgttatt
     3961 atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc gtcagtatcg
     4021 cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata tcgagttgtt
     4081 caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa acattccttt
     4141 aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc tttaccgatt
     4201 gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc ccaataatga
     4261 acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg tctccatgcc
     4321 tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg gcggaacgac
     4381 gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc caggacaatt
     4441 accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt agaacaagtt
     4501 tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc cgtatggtcg
     4561 aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc cacctaagtg
     4621 cgtcatcggt cgctgaacag cttccagaaa gccaatctcg tatatttaaa acactggttg
     4681 ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc gaattgaatc
     4741 taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat ttaaaagatt
     4801 tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg aaaaaactat
     4861 ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc tcggctggca
     4921 gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg agcggacaat
     4981 ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg ggatgttgtt
     5041 tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat tcattcctgc
     5101 tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac cattgacatc
     5161 cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg ttttaggata
     5221 aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag gaaaagtttt
     5281 gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt gtttttgaga
     5341 ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa ctaacaatcg
     5401 ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt tacttttttc
     5461 tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca gagaaagtgt
     5521 cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac gcagtgcagc
     5581 cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag tcaatacacg
     5641 acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt gaagtgtttt
     5701 ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa gaggcgtcac
     5761 cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag tagatggata
     5821 agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga attccgtgcg
     5881 tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag agaatagaag
     5941 cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt ttgatttatt
     6001 tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt gtgattcaaa
     6061 gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa actttccatt
     6121 tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat tgcaccgacc
     6181 accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca agagtacgcg
     6241 tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag cgccgactac
     6301 gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca tatctgaaga
     6361 aagaaaacag aaaaaaaatg ttttgagcca aaagagaaaa tgagctaaaa tgagggaaga
     6421 taagcaaagg agcgtggaac gatgacaaac gcaacagaat atgttcaagc aattcaagaa
     6481 aagctacatc aaaaggatca agggcaagaa gaatttttac aagcaatcga tgaattcatg
     6541 cccacggtta tgtctttttt ggacactcat cccgaatata tcgaaaagaa tatcttagaa
     6601 cttttaacgg aaccagaaag agttattcaa tttcgagtgc cttggcaaga tgatgaagga
     6661 aactggcgag taaatagagg gtaccgtatc caatacaact cagctatcgg cccttataaa
     6721 ggtggattgc gcttccatcc tagtgtcaat ctaagtatct tgaaattttt ggcttttgaa
     6781 cagattttca agaatagttt aaccggtctg ccaatcggtg gaggaaaagg cggaagtgac
     6841 ttcgatccta aaggaaaatc tgataatgag atcatgcgtt tttgtcaaag tttcatgctg
     6901 gaattgtcaa aacatatcgg cccatcgatt gatgttcctg cgggagatat cggtgtagga
     6961 gctagagaaa tcggctattt gttcggcgaa tataaacgat tgaaaaaata tgatactggt
     7021 gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga tacgtacgga agctactggt
     7081 tatggattag tgtattatgt caaacatcta ttgaacgaag aaaaagattc atttaatcaa
     7141 aaaacagttt ttgtttccgg aagcgggaat gtagccattt atgcaatcga aaaagtgcaa
     7201 gagctaggcg ggaaagttgt tacatgttca gattctagcg ggtacatcta tgatccagaa
     7261 ggaatagatg tgaaactatt gaaagaagtc aaagaaatca atagacaacg tttgacggaa
     7321 tacgcaaacc aacgtccttc tgctatttat catgctggag aatctgtttg gacattgaaa
     7381 gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg aaatagacga aagcttggcc
     7441 gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag gcgctaatat gccatgtaca
     7501 gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt attgtccagg aaaagctgct
     7561 aatgctggag gagtagcagt atctgcttta gaaatgagtc agaacgtcca gcgattgact
     7621 tgggaaagag agcaagtgga tgagcagctg gatcaaatca tggaaacgat ttaccatacg
     7681 tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc tgcttcttgg agcaaatgtt
     7741 gctggatttg aaaaagttgc caaagcaatg gcagcagaag gtttagtttg aaaaatgaaa
     7801 ctttcttgga aatattgtca gcattttgtt tatttgcttg aaaaataata aaaaaaaacg
     7861 taaagtatta cctgtagagt aaacctataa ggaggagaac aaatggcaca cattcatttt
     7921 gactattcaa aagtagcacc gtttgtcaac gaacaagagt tagggtacat gcaaagccaa
     7981 gtaacagcag cccacaacga attacgggaa ggtactggcg caggaagcga cttccgcggc
     8041 tggatcgatt taccaacgaa ctatgacaaa gaagaatttg cacgtatcaa agaagcagct
     8101 aaaaaaatcc aatctgattc tgaagtatta gttgttatcg gtatcggtgg ttcttattta
     8161 ggcgctcgtg cagcgatcga cttcttgaat cacacattct tcaatttatt agatagcaac
     8221 acaagaaaag caccacaaat cttctttgcc ggaaattcaa tcagctcaac atatattgct
     8281 gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa acgttatttc aaaatctgga
     8341 acaactactg aaccagcgat tgcgttccgt gtattcaaag agcttcttgt taagaaatat
     8401 ggacaagaag aagcaaacaa acgtatctac gctacaacag ataaagcaaa aggtgcagtt
     8461 aaagtagaag cagatgcaga aggttgggaa actttcgtga ttcctgatga catcggcgga
     8521 cgtttctctg tattaactgc tgttggtttg ttaccaatcg ctgttagtgg tgcagatatc
     8581 gatgcattga tgcaaggtgc cgctgatgca agcaaagcat actcaagcga caaactagaa
     8641 gaaaacgaag cttaccaata cgcagcaatg cgcaacattc tttaccgtaa aggcaaagta
     8701 actgagttgt tgatcaacta tgaaccagga atgcaatact tctcagaatg gtggaaacaa
     8761 ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct atccttcaag cgcaaacttc
     8821 tcaactgatt tgcactcttt aggacaattc atccaagaag gacgtcgcaa tatttttgaa
     8881 acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa ttcctgaaca agcagaagat
     8941 ttagacggtc taggctactt gcaaggaaaa gaagtggact tcgtaaatac aaaagcattt
     9001 gaaggaacat tgcttgctca tacagacggt gacgtgccta acttgctagt caaaattcca
     9061 gaaatggatg cttatacatt gggttataca atgtacttct ttgaaattgc tgtaggtatt
     9121 tcaggttact tgaatggaat caacccattc gaccaaccag gtgtagaagc ttacaagaaa
     9181 aacatgttcg cacttctagg caaaccaggc tttgaagatc ttgctaaaga attgaacgaa
     9241 cgcctgtaag ataagggttt ttcgctagat ttaaataatc agtgatagaa acatgttcgc
     9301 tttaaaatga aaactggtac ccatttaggt actcaaacat actgcatcgt ttattcagaa
     9361 taaggggctg tgacaagacg tttgtcacag tcccttgttt gtatagaaat cgattgattg
     9421 ccttgctctt tagtttgttt taggacttta agaacagtga ggctgacttc gaaggttaat
     9481 tctgtttttt tgttttctag aatcagtaag caaaaaagca ctcacttcga gtgctttttt
     9541 gcgatatatt cttagcttaa gataggccta atttttcttt tacagatgag ggtaccttat
     9601 cgctggtaat tacttttttc tctatgatcc gcttttcgct tgctttcaac tgcaagatcg
     9661 ttccttcggg gtaagcagaa ccattggcag agtcaccaga agtaatgaca tcgaaatcta
     9721 actgtctttt ttcaccgttt tcagcataag catctacgtt gtatttaaaa cctcttccaa
     9781 ttacttcccc attatctgct ttgatatcag tttctgcagg cattggagct ttgactaccg
     9841 cataataatc tttacctaca taagtagact gatagtagtc ccaacctttc catcctataa
     9901 aagcaatcaa tatgataacg atcaacccta aaattttttt catgaaagat ctcctttttt
     9961 atttttat
//
LOCUS       DACDBG010000090         9964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDBG010000090 DACDBG010000000
VERSION     DACDBG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.090"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IVZ90_002333"
     tRNA            8..81
                     /locus_tag="IVZ90_002333"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IVZ90_002334"
     tRNA            101..173
                     /locus_tag="IVZ90_002334"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IVZ90_002335"
     tRNA            201..274
                     /locus_tag="IVZ90_002335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IVZ90_002336"
     tRNA            288..361
                     /locus_tag="IVZ90_002336"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IVZ90_002337"
     tRNA            385..474
                     /locus_tag="IVZ90_002337"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IVZ90_002338"
     tRNA            487..560
                     /locus_tag="IVZ90_002338"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IVZ90_002339"
     tRNA            579..654
                     /locus_tag="IVZ90_002339"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IVZ90_002340"
     tRNA            656..726
                     /locus_tag="IVZ90_002340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IVZ90_002341"
     tRNA            740..813
                     /locus_tag="IVZ90_002341"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IVZ90_002342"
     tRNA            838..925
                     /locus_tag="IVZ90_002342"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IVZ90_002343"
     tRNA            947..1018
                     /locus_tag="IVZ90_002343"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IVZ90_002344"
     tRNA            1041..1130
                     /locus_tag="IVZ90_002344"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IVZ90_002345"
     tRNA            1143..1216
                     /locus_tag="IVZ90_002345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IVZ90_002346"
     tRNA            1235..1307
                     /locus_tag="IVZ90_002346"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IVZ90_002347"
     tRNA            1311..1383
                     /locus_tag="IVZ90_002347"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IVZ90_002348"
     tRNA            1388..1458
                     /locus_tag="IVZ90_002348"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IVZ90_002349"
     tRNA            1471..1544
                     /locus_tag="IVZ90_002349"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IVZ90_002350"
     tRNA            1556..1627
                     /locus_tag="IVZ90_002350"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IVZ90_002351"
     CDS             1893..3086
                     /locus_tag="IVZ90_002351"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8263355.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IVZ90_002352"
     CDS             3216..4688
                     /locus_tag="IVZ90_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8263356.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IVZ90_002353"
     CDS             4771..5559
                     /locus_tag="IVZ90_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8263357.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IVZ90_002354"
     CDS             complement(5626..6294)
                     /locus_tag="IVZ90_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8263358.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IVZ90_002355"
     CDS             6409..8121
                     /locus_tag="IVZ90_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263359.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IVZ90_002356"
     CDS             8124..9896
                     /locus_tag="IVZ90_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263360.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACDBG010000091         9777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDBG010000091 DACDBG010000000
VERSION     DACDBG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.091"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IVZ90_002357"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IVZ90_002357"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8263361.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IVZ90_002358"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IVZ90_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8263362.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IVZ90_002359"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IVZ90_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8263363.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IVZ90_002360"
     CDS             2857..3732
                     /locus_tag="IVZ90_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8263364.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IVZ90_002361"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IVZ90_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8263365.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IVZ90_002362"
     CDS             4629..5063
                     /locus_tag="IVZ90_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8263366.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IVZ90_002363"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IVZ90_002363"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8263367.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IVZ90_002364"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IVZ90_002364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8263368.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         3426 a         1687 c         2192 g         2472 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaat
//
LOCUS       DACDBG010000092         9673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDBG010000092 DACDBG010000000
VERSION     DACDBG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.092"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            321..1556
                     /locus_tag="IVZ90_002365"
     CDS             321..1556
                     /locus_tag="IVZ90_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8263369.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2555..2815)
                     /locus_tag="IVZ90_002366"
     CDS             complement(2555..2815)
                     /locus_tag="IVZ90_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8263370.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2842..4143)
                     /locus_tag="IVZ90_002367"
     CDS             complement(2842..4143)
                     /locus_tag="IVZ90_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8263371.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4435..4893)
                     /locus_tag="IVZ90_002368"
     CDS             complement(4435..4893)
                     /locus_tag="IVZ90_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8263372.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4906..6231)
                     /locus_tag="IVZ90_002369"
     CDS             complement(4906..6231)
                     /locus_tag="IVZ90_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8263373.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6248..7660)
                     /locus_tag="IVZ90_002370"
     CDS             complement(6248..7660)
                     /locus_tag="IVZ90_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8263374.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7677..8441)
                     /locus_tag="IVZ90_002371"
     CDS             complement(7677..8441)
                     /locus_tag="IVZ90_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8263375.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8545..9420)
                     /locus_tag="IVZ90_002372"
     CDS             complement(8545..9420)
                     /locus_tag="IVZ90_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8263376.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3138 a         2003 c         1456 g         3076 t
ORIGIN      
        1 ttctttctta cgtgtgtata atgacgacag cacacctatc aattaatata attattttat
       61 ttttttatgt tctcatgttt atttttaatg acacaccttc tcgtatttat ctggcacaac
      121 atataaatcg atcaaaaaat tgttgcgtgt ttttttgaaa attggagttt caacttgatt
      181 acaaaattta aatttagaag ttgtgaaaac agcgagagac agtttaatat tcaaaatata
      241 cataggctac tcgaacgctg tgttttttag tatactagta agaaacgaag ggatagactc
      301 attccaataa ggaggatttc atgaagacaa ttctttttgc tcctgcagta ttcaatttag
      361 ctgagacaac acgaatgatt gaggttgcca aagctatgaa agaccgttat tactgtgtat
      421 tctttggctt ttctaaaact tttgctcctt tgatcacaga aagtggcttt gagtatcatt
      481 tactagcgcc gatccttacg aaaaaacaag aagaacaaat cataaaattt gaccagttgc
      541 gtacacttaa aaaccctttt accaaagaaa tggttgcctc tcgtatcact agtgaacaga
      601 gtctgattgc taagtttgag cctcgactga ttgtgacagg ttcgaatgtg acaatttttt
      661 tatcggcccg caataaaaag attcctttag tatatgttaa accctatgct ttgagtgaac
      721 ctttttttca atcagagcat gcgctgatgc cccacgagtt acgtttcttc ggccctttaa
      781 gcaatcccct ttggcactgg acaaaaaagc gtatagtaaa tacccgatgg atccccaatg
      841 ccttcaaaca cgctgcacaa aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga
      901 tggatgctga tctaaatctc atcacaacgc ctaaactatt tttggaagaa gaacagctgc
      961 cggaaaatta tcgaatcgtt ggtccaattt ttgctaagct cagtacttcc ttgcctgaag
     1021 aagtgctaac ttttatacag cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa
     1081 tgggtagttc aggcaatccg cgtatgttac gcaaaatttt gttcttctta caaaaacaac
     1141 cggatctagc ggttattgca cctattgact atctgcttga tgacattcaa tctgactcag
     1201 aagatctttt aataacagaa tatctgtcta gtcatctttt gcatgaattc attgattttt
     1261 cttttatcca cggcggggaa gggaccgtcc aaactgcctg tgaatccggc aaaccgttta
     1321 tcggtgtggg gatgcaattt gagcaaagct gtaatattga ctattgtgta aaatacggaa
     1381 acgcaatcgc cctcacaaaa ccagtgactg aaagaaaact gcagattgct ctgcgcgaag
     1441 tttcttcacc caagattcgt cagcaggcac gcacgctgca agccgttttt ccaataaatg
     1501 gtccacaaca agcaattcaa gaaatcgaaa ccttcctgag caaccaccca atatagtttc
     1561 aagtagaaaa agctttttta atagagactc atcagtaatt tcgctattcg agacccagac
     1621 agtgaaaata caaaaaaagt aaatagtgga ttgaaaagtt agagttactt aaattacggt
     1681 gtctaaaaaa cacaaaatca tccttaaaat aaaagatctg acgctatcca aatgagcata
     1741 ggtagcgtct gccatttatt cttagtcaaa ttctttgaca cctttctctg gttggatcat
     1801 ctcaacgttt cttcaaaaga aagaccataa taattaggca aatacatttt cttaaaatgt
     1861 gtcatataga tctagaagca tcgctttgta gagatttgtg ccgctagtcc cctcaacaat
     1921 tactaaatca aatgtaggat ttaccacttt atctttgata atcaacctcc ctcttctgtt
     1981 gagacaatga tctcgcaatc cgatgcatac tcctatataa actgccagca atccccacat
     2041 attgagtaga tcatgtcaga atgttctatg tcgtcagcaa cgatacacta aaagtcacgt
     2101 tcaccatgac tgattgctat tcccagcaca attatttcta tacacgtttc atgatttgaa
     2161 taaacactca catcatacat attaccattc atacaccata ctgcagcccc tactatatgg
     2221 ttgtaagctc ccaaatctat atttgacttg atcactttgg tagtagatct actaattcta
     2281 aatccattgc tgataaaatt tccatttttt cctccttctt tctgccaaaa cagttaaaac
     2341 aataataatc tttatgtaaa aaagggctgt gacaaaagcc ttgtcacagt cccatattcc
     2401 gaataaacgg tggtcaaaag cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa
     2461 gccaacattt cacaaaaatt taagaagcaa ttttcgtaaa tttcttctta tttcttgaag
     2521 ctaaccactt ctgttacaac cacttttaga acatttaaag cttcgatgtg acaaaccatc
     2581 tatgctgctc gatcataaaa ttgatagcaa aaattccaca aagtacatat gtcccccatg
     2641 aaaacaaaac attcatatgc ggtacaatga tctttggaag aatcacgatc aaaaatgatg
     2701 ccagtgccca aatccatcga ttgatttgaa tatgatatgt agctagcata ttcaatataa
     2761 ggtgactagc cgttaataaa aagataaaaa caaaaaataa aatcagccaa tacattgttt
     2821 cccaccttct aaaaaccaaa cttagacagt ctggacttcg ttttctccac gactttgttc
     2881 atctgacagc atctttttat cataagcttt tacaaatgga taatagataa taaatgctat
     2941 caatgtgttg atcatgatca agataacaac acgccagtca tttcctgctg aaagataacc
     3001 gccgattgga gctggtaatg tccatgttgg caaaacagta aatctattga ccatttctaa
     3061 tttgactgca aaataactga taatagtcaa aactaaagga actaagttga acgggatgaa
     3121 catgtaagga ttcaacatca taggagctcc aaaaattatg ggctcattga tattgaagat
     3181 accaggaata atagaaaaac gaccaacctc tttcaagaat tttgatttac agaagaatat
     3241 aaacaaaaca gataccacga tcgttgctcc tgcaccacca attgttaccg tccattgata
     3301 aaactgttca ggagcgatat agggtaattg ggtggcagct gtaccatctg ccaatgcctt
     3361 tgcattctca tccaacatga tcagccacat tggacgaaca acagccccta caatactcat
     3421 gccatggata ccaaatgacc acaacaaatg gatcaaaaac attggtagta aaacaccaag
     3481 caaattattc cctaaaatat tggttaaagg ctcgaataac gataataagg cagcgtttac
     3541 atcaaagccc gcgacgcttc gaatcagcca agtaattgtt aaaatgactg cacctggaat
     3601 caatgcctca aaactacggg caacagcagg tggcacttgt tctggcattt tgatagtaat
     3661 atttcgattt ttaaagaaac gatatacttc cactgcaaaa atcatagata agatccccgc
     3721 aaacatccca gatgctccca aagaactcat aggtaagacc cagccaagag gtgcctcttc
     3781 aggagttaaa acgtttacag gaacttgtaa cataaagaac gttcctaaag ataatatacc
     3841 acctgtcaca ctatctaatc catatgattt agctagatcc gaacccataa caaaagttgc
     3901 ataaatagcc atcaatccca tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg
     3961 ggcaataaat tcattccatc caggaaacgg taaattgata aagaccataa aaaatgtccc
     4021 aatcaaaatc aatgacaatg ttgaaacaac cccattacga attgctaata aatgacgctg
     4081 tgtaccaatt tttgccattg gtgggacaag tttctcttcg acccatttca tcagcttttc
     4141 cactaaaacc actccttttc aataaaatat gaaccattca ttgcatgaca acgcaaagaa
     4201 tgttcataac taattatata attaagaaat tttatttcaa tatattctct taaataatca
     4261 aatttatttt taagaatcct gaaaataagt tttctaaatc ataggagcaa caaaaaaatt
     4321 cgataattaa caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa
     4381 aattttgtaa attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa
     4441 ttcaaaacaa ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa
     4501 aatgcctgga gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa
     4561 atctataata gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg
     4621 aaacggactt ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc
     4681 agattcactt tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt
     4741 aactggcgca tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt
     4801 tgaagaaaag acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc
     4861 ttttagaatg tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg
     4921 ccaatatttt ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt
     4981 tggtacggtt tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta
     5041 agcatctact agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc
     5101 actgattggt tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat
     5161 ttcaacaact gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat
     5221 aaagcgacga cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg
     5281 ctccccaaaa ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat
     5341 ttctttggta tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc
     5401 agtatgctca acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt
     5461 acttggaaaa tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc
     5521 tcctatatta aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg
     5581 cataatttct cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa
     5641 accataatac gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc
     5701 ttcaattgaa atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg
     5761 aacagcttca gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc
     5821 atagtcttgt atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc
     5881 aaatcctccg agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc
     5941 gtccttttct cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc
     6001 tgtaaaagca agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct
     6061 tttcagcata aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata
     6121 taaacgtatt tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc
     6181 tggtgtatac gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt
     6241 ttcctttcta ttcttctaat aatttattga attctgccat aacataagtc acttctaagc
     6301 cgatgataat ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct
     6361 tgattcgctg ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac
     6421 aactatagac atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca
     6481 tatattcata atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc
     6541 caaactctgc cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat
     6601 aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat
     6661 tggtagcgat tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa
     6721 ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca
     6781 cgtacaacat aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa
     6841 taaatgaagt tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat
     6901 aagctgcttt atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa
     6961 aaactgataa aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc
     7021 tgaaaacttc cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca
     7081 acacatggtg cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac
     7141 ctaatgctcc catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg
     7201 gccagataaa gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg
     7261 ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat
     7321 tatacgtcca tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt
     7381 ctgggtccat aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg
     7441 caggtattcc tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga
     7501 tatccaagtt tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat
     7561 cgtcagctga taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca
     7621 tcgctcttcc aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa
     7681 atatcctcat atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct
     7741 gaatgaatta aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac
     7801 aattcctgat ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat
     7861 atcatgagta aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca
     7921 tcagtttctc ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct
     7981 tttcgttcaa agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc
     8041 tcctgatttt catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc
     8101 cccgcatgat aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca
     8161 atgttaaatg gatgctgata ataagataag gatctgaact ctccgctttc gataagagta
     8221 tcgacagttt tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc
     8281 ctaggcattt cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat
     8341 gtagttgatg tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg
     8401 ctatagccaa aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt
     8461 ctactaaaat aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg
     8521 cctatagata gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat
     8581 gatttcattt tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag
     8641 tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac
     8701 tggcgtttct tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg
     8761 atgataagtc agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa
     8821 aatcgtggtc gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc
     8881 agcattaatg atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata
     8941 tcgatgattg attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa
     9001 tctcatttct ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc
     9061 aaaataaata tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct
     9121 agctgttatc agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga
     9181 gactatctca ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc
     9241 atcctcaaca tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc
     9301 aattttttct attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac
     9361 atacaccgtt aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat
     9421 acatattttt gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt
     9481 cttaggtcgt gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac
     9541 ataataaaag cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt
     9601 gatttggagg taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc
     9661 tatattgata att
//
LOCUS       DACDBG010000093         9665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDBG010000093 DACDBG010000000
VERSION     DACDBG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.093"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..203)
                     /locus_tag="IVZ90_002373"
     CDS             complement(<1..203)
                     /locus_tag="IVZ90_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8263377.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(640..900)
                     /locus_tag="IVZ90_002374"
     CDS             complement(640..900)
                     /locus_tag="IVZ90_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263378.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            975..1178
                     /locus_tag="IVZ90_002375"
     CDS             975..1178
                     /locus_tag="IVZ90_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8263379.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="IVZ90_002376"
     CDS             complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="IVZ90_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8263380.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1807..2706)
                     /locus_tag="IVZ90_002377"
     CDS             complement(1807..2706)
                     /locus_tag="IVZ90_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8263381.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2719..3312)
                     /locus_tag="IVZ90_002378"
     CDS             complement(2719..3312)
                     /locus_tag="IVZ90_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8263382.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(3553..5757)
                     /gene="topB"
                     /locus_tag="IVZ90_002379"
     CDS             complement(3553..5757)
                     /gene="topB"
                     /locus_tag="IVZ90_002379"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8263383.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5768..7228)
                     /locus_tag="IVZ90_002380"
     CDS             complement(5768..7228)
                     /locus_tag="IVZ90_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8263384.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(7289..9661)
                     /locus_tag="IVZ90_002381"
     CDS             complement(7289..9661)
                     /locus_tag="IVZ90_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263385.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2372 a         2010 c         1641 g         3642 t
ORIGIN      
        1 cgtttcttta ctttctcatc aatcaaaaca taatcgccat aagacaaggt atattttttc
       61 ccgcatgttg gacattgata caaacgatag cttttgatac atttcgctct ttctccacaa
      121 tcgcattcga cagtatgtgg tgcatgatag tttgggaata attcgctgaa gggaacaccc
      181 caataatcaa atttctttcg catgattgat tctgcctcct cttaggaaag gttttatttc
      241 ctttcgttag ttctttcttt attgatttgt tggccagaat gaatcaacga aaagcgacaa
      301 ggacaagggc gcgcagcgtg ccatttaccc ttgtccttgt cgctttttgg tgataaaagg
      361 caacttgttg ccggtctggc aaatcaataa agagatagcg aacaggaacg aaccttttgg
      421 tgagaggaag ttttttctat tttgtcttgc tttgcaagac accatatctt agtgaacgtg
      481 caacgttcac ttcctttctt ctcgaacggc ggtggcggtt ggagtgagag aaaggttttt
      541 caattgagaa ggcacttgtg tcttcctaaa aaggtctttt cctgtttcta tgtaaaacaa
      601 aaagagctgc aatcctcatt taggactgca gctcttcttt tatatgccca tcgatcgcgt
      661 aaaatgatcg gggtttctaa tttgcatcga ttcaatgact aattgattcc gtttattgaa
      721 atgacctgtg acggcaatat tgtattttcc atcatccata attaacaatt tatcgataat
      781 ttcaatatct gcaactacac aattaatcga ttcatgaatt gtttgtaaag taaagcgaac
      841 caataatggt tgtgttttta ttactcggat tttatcaagc aaaccaacat atttgttcat
      901 cacgatcacc tcgctaaaat tatagcaaaa acagctaaaa ttgagaattt ttattcgatt
      961 ttagatatgc tataatgatc aagacagtaa agtttgtgac ggtggctatc cgattgaaag
     1021 tgaggtggtg cgtatgtgcg tacttccaaa atcttacaga aaggagaagc cttttgcctg
     1081 ttgaaacggc gctaggatta atgattagtt tcggttcttt gatcgcaaca atcatttttg
     1141 gaatcttagc agttgtaaac aacaatcaaa aaaaataacc gtcagcttta gccggtcgac
     1201 gattattttt caaaaataat attaagctgg tcaccgtctt aaacggtgct gttaggggag
     1261 ttgttagcgc aactcctctt ttcgtatcca ttataacaac tttattgatc ttttacaatc
     1321 agaatggtaa atcatcatca ctaatatcga ttgattgtcc gccaaatgga ttttgtgaag
     1381 cgttcaatcc attgttgtta ggcgtagcta catcccgttg ttcaaaggtg gtagggcttg
     1441 gactgctgga cgattgatca gcgtgcgcac ggctttctgt cgctgcttta ctttctaata
     1501 gttggaaatt atcggccaca acttctgtca catacacacg ttgtccttgt tggttatcgt
     1561 aggttcgggt ttgaatccgt ccaacaacgc cgatcaaact gcccttttta gctaatgtcg
     1621 ccatcgtttc tgctggtttg cgccaaatca cacagttaat aaaatcagct tctcgattgc
     1681 cgttttgatt cgtgaaattt cggttgacag ctagggtaaa agtcgctact gcagtaccac
     1741 tcgcggtata acgtaaatct ggatctttgg ttaagcgtcc tactaaagtt acattattga
     1801 tcatctttat ctctccattt cttgattcat ttgttgtgat cttttaaact gccgttttct
     1861 ttcttgattt tcaattttga tcacactttt cattaggtga tcaatttcgt tgactgtatc
     1921 tgtaaatgaa atggaattcg caaacgtgtt aactgagcga tatttttccc actgcttttc
     1981 caaatagtca tttcctctga tcttatccat ttctctttca taataaaagg acaacggttt
     2041 tgtatcccca cgttttgtaa atgtatttcg aacacttttg tataactctt tcgtatcgat
     2101 attttctagt ttcggaatgg taaataagtc gtagtaatct tttgctctcg aattatctgt
     2161 gccaaagctg aacgtagcac ttagcttttc tgcaataatt tgttctgttg gataagctgg
     2221 aatctccact tttttatctt caaacattaa ttggtgatag tgtgtatcta cttcaggata
     2281 aatagaatct cctgttgtca cgtccatttt aaacggtaca cgtactttct ctaaattagc
     2341 caccaattta atttgaaatc caggataaaa atcggcttct cttgtttcct tcattccttg
     2401 aatagagaaa acaatgcctt cttttgtggg ttttctagtt aaatcattaa aaatgctctt
     2461 caatttttct tcagttaatt tgctatttct aaatgtcgta tcgatatcaa ttgttgtcct
     2521 tctatcgatt ccatattttg atcctaataa aaagccgcct ttcaaaatga agtcattttt
     2581 gtactttgat tcagaaattt gttcaattag acgatccatc ataaaatgcc gttgcactaa
     2641 ctgtgcattt aatcctgtac tctttgctaa atttcttgtt tttgctgcaa attgtgtcgg
     2701 tgtcatcttt ttcctccatc aatataagct ttgcatgtgg tatttcattt ctttttctac
     2761 atgtagtttt gtcatgtaat ctcttaattt acttggatct ctcaatgggt cttccatgta
     2821 ctcttttaat gcttctaatt tcatttcata ttcaaattca tatctcggat tccaaatatc
     2881 acaaagtgta cgctctttat catacatagt aataggattt ccttgaaagg aagttccctc
     2941 tacttttcct agttcataaa cttctttctt cacgaaatga aattgtaact gctgctcttt
     3001 tgtaattgaa cgatcaacgt tatagccatg gggaaaagtt aagtgaatga atttcggcat
     3061 attcgtagaa agctggtaaa tatctaaagt cgtttcatga gagaagattc ccctggaaaa
     3121 tcgttgctga agccaaaata aatcatctat tccatgacca tcctctacat aataaccacg
     3181 atcaactttg atcaattttc cttgattcac caatctttgt acagtggttg ctgcgattcc
     3241 aaatttcctg gcttccttca tagataaatt tccatcgcga atagccaaca attctttcag
     3301 tttatcttgc atttacttcc cctcccttcg ttacaaatac actacatcaa atatatcata
     3361 gtagtttatt tgtaacaaga ttaacgtcta tcgatctaat tcgatttctt tttgaatgcg
     3421 ctgctggttc tttaattgtt gcagtcttct tttatttgca cgtaatttct cttgttctaa
     3481 acgaattttt gctttttgat tttcgatttc ttgttgtttg tgtgttgtat ttcgcatagg
     3541 ataacccctt cattattttg tagtgcggcg tgttcttttc ttatagttcg tttttttctt
     3601 ttcagaaaat tcaaagcctg ttgtaaagtc ttcttttaaa acgatcgttg cttcaaacga
     3661 acctttctga ccttttagtc ctctgactga ttggcttgtt cggccgtttt gaatgatctc
     3721 tttcagttgt gtggctgtga gtttcttttt ggcaatcgtt gtccataatt taaaatcaca
     3781 ggcttcagac gtacatgccg ccacattttg ataaagcaag acttcttgtt cccgacattt
     3841 tggacaaaca cctaaatgac gcgttgcttc ctgtttttca ctggctgctt tcacctcttg
     3901 taacgttgat ccaaaatctg ttgactggat ttgtcccggt acgacttcca acaagtgaga
     3961 gacaaatttt tggatattag ctaagaaagt agcttgtgtt cctttctgct caccaatctt
     4021 taataagtaa ctttcccatt tggcggtcat ttcggcactt gttagcagcg gctctttttc
     4081 cacggcttgg caaagtaaaa tccctttatt tgtcaccaca agcttatttt tttctacctt
     4141 gatatattcc ttttgtttca aagtttcaat aatatttgct cgtgttgctt ctgtaccgat
     4201 tccttctact tctttcaata tggattgtgc ttcttcgcta tccacggttt ttccagctgt
     4261 tttcatcgct gtgatcaacg taccttcggt ataaggttgt ggtggcttcg tttctttttc
     4321 aaggacttgt agatctaccg tcaccgcttc tccttttgtc actttaggaa gtagcggttc
     4381 ttcttccttt tcttttctgt cgctctcttt gccaaacagc tttttccaac ctagatctac
     4441 tggtactttt ccgactgtct ttagctgaag ctgctgaact tgcgtcaaga tcgttgtttc
     4501 ttcataggta tacttttccg caaacatggc caccgttgtt tttaacactt gttcataaat
     4561 agcctgctgc agtggactta attgttcaaa agccgctgcc gtcatgaccg tctttgtagg
     4621 aatgatcgcg taatgttcct ggactttctt attattcaca tatcgactat tcggtgtatg
     4681 aataggagta ggaatggctt cggcctttaa aaagtgtttg tattcgtcta agtgatccaa
     4741 cagatacgca tactcccctt ctgtaatata aggggtatct gttcgtgggt agcttagata
     4801 tttcccttca tacaaccctt gcatggcttc caaagtatct ttggcactag ctttcatgag
     4861 ctgattcatt ttagattgaa ggctggatag agaaaaaaga cgtggacttg ccgcactttt
     4921 tttctcggtt tcaacttggt ggatcgtacc tggctgtttt cccaattgaa cccccttttc
     4981 ttttaaataa tcttcaaatg cttcttgcgt cgcttgggtt tcattcggat caagcttcgc
     5041 atcaaatgcg ccattttgag cgattacttg ggcttttcct tcaaaatatg gttctttttg
     5101 gaagtttcta atcttttcct gtaattgata aatcatgtaa agcgttgggg tttggactct
     5161 gcctaaggaa aaacttcctg gtatgccttt ttgctgcaag agtaaactat acagcgggct
     5221 accgttcatg ccgatcaacc aatcggcgat ctggcgtgct tgtgcttctt caaattttgg
     5281 gtaggtctct tctgcatctc gtaactgctg gaatccctga taaatggctt ctttttctaa
     5341 ggagttgatc caaagtcttt taaatatctt tccatgatcg gcgcctgctt gttgaatgat
     5401 cgtccaagct atcagttccc cttcacgatc gctatctgtc gcaataataa tggtgtcggc
     5461 cgattgaagt tgctgtttca ccaccttgaa ttgctttttc ttatctttag gaacatgata
     5521 gtgataatgt gtagggaaaa taggcaaatg ctctaatgac cattgtttcc aattttcatc
     5581 gtacatatcg ggcgaatcta actcgactaa atgaccaaaa ccatacgtga tcacagtttc
     5641 tcctgaaaac aatcgatctt ggatctcaaa atacccatct tttcgggtag ctttagaaaa
     5701 gctatcggca taagccttcg cttgtgacgg tttttctgct aaaataacgg ttttcatcat
     5761 gacgatttca ctccctttct tgctgtctac cttgttgaaa caacatggca cgtttttgct
     5821 ttctgatctc ttgttggtag atttcatgct tcaattgatc attccaatcc ttttttactt
     5881 caggtacaaa ttcatgttca tgatttttat agcctttgta gtattgttct gctttataag
     5941 agaaaagaat ctgagcatta atatcccctt tttcttgata aaaacgatca aaatcgtacg
     6001 taccatccac gcgttcacaa gcttttaatt cttttgcgac ttctctacaa atatctttca
     6061 aattaatagc ttctattgaa tcgttaacag aaagctgctg accaaaagac gcatttactt
     6121 tgtgagcgga aaccaattgg ttcgttaatt gcagattgtt ttctgtcttg tgtatggcca
     6181 taatagcttc tggtgtgatc ttttcttctt ttgcgacttc ttcataaatg actttgtatt
     6241 cttttggcac ttgccaatca ttagcgattc ctcgttcaca acgtactttc tgattcgtaa
     6301 atggatcaac tgcgcccatt aattgttctt tttcgtagaa aatatgtcct gcacggtcat
     6361 tatccacaca aatctcgatg gattgaggaa aggcttgttt ctttctcaat tcagatactg
     6421 cctcaccaaa ataatgactg atcacatggt gttttaaccc ttccatcgaa actaaccacg
     6481 tatcgtttaa ctgatcacga tttagtgcag catagctaag taaatcaata ctactctcaa
     6541 aaaattttaa ctgcttaggg tcaccgatct ttaggttaaa gccatgattc gctgtcgaat
     6601 ttttatctat atgcttatat gtgcctctct caccaaaacg ttcataatca atgaacgttc
     6661 cttgataact cgcccctact gtctcatttg tctgtggatc tttccataaa aaggcgaccg
     6721 catgatctaa acgatcgtct cgaactaaga aattttgccg ttcatgtttt ggctttagtt
     6781 cggcaatcaa cccttctttt tctgcataat caatcagcca ctctggtagt tttcgttctt
     6841 ccttcagata attccttcct ttttctgtga gtggttgata atcaacgggg tgatctttgt
     6901 acttttctgt ctcaaaatga agattttcca ctcgttctgt tttgtcttca ttctttagaa
     6961 taaaatcagt ggctgcttta aattgttgct tagggtcttg gatctctttg tctatgaaaa
     7021 aaagtttagc taattcaata atgtcgccac taatattttg actgttccaa taaaaacgtt
     7081 gttttcttcg atcaaaaaca aatgaatcat ggtcttctaa gcgatagtct cgtcctttat
     7141 tgactacagc cattccattg tttcgagcaa aatccactat atcgatctgt ttacatcgat
     7201 caataagact catttttttc tcggtcattt taaaacctcc caaaagaaaa aagactatcc
     7261 gtttttcaga tagtcttttt tacttgtttt atacacgttc tttttgtgac aattgttgtt
     7321 cataatagat ttcatttttt cgttttgcca cttctgacac tgtctcaagt gaaaatcgga
     7381 atgaatttcc tagcgcaaat tcttctgaag gtaacgccgt ttggatctct tttccataac
     7441 tttttccttt taaaattact tgattatgct gtggatcttc aataaacgcc gcatccttca
     7501 aatgtaacgt tcgacccgta tcttcttcca ttactttccc gtctttcttg tcgatccaaa
     7561 gtgaaccaac aggaacaata ttcttttgtt ctgccgtttc tacagtattt ttatgctcct
     7621 gcttcacttc tgctggttgg atctctggtt ctctttctct gtattgattg atttcattag
     7681 aaatggtttg aatgaattca tttgcgacac ggctgacatc attcatgatt ttcccacgtt
     7741 ccgcagcttc cttatcatcc aacgtctgac cattcttcgt ccatgctgca atataaggaa
     7801 tagctttctc cgaagtgtcc attccaaaat gtttgcacac aatataagaa gtcatttctg
     7861 cctgcaattc tttagttgcg gtatctaatt ctgaaaattt gtgcattctt gcatgggcta
     7921 attcatgaat acttgtagct aaatgctgcg taggagtatt tcgagtattc atgacgattt
     7981 cttcttcgcc agtaaattcg ttacgaacat atgccccccg tgcttgtcct aattctcttt
     8041 gatagacttc actttctgtc atatctcgaa tttgtattcc aatattgttg gctaccgctt
     8101 gaatcccagc ttgaagttct gactgtcctt ttttatccaa ttgaaaatca aagacacggt
     8161 tcggaaagat tttcggatag tcttcaggtt ttaactgtgt ttgtgtgata tcaaacactt
     8221 tttcaatcgt gtagtaaggt tttttgatca cttcgatttg accactttcg attcgttttt
     8281 tatcttctgg tgtagcttct gtttctcgaa caaagccacg tcctttctca taaaatcctt
     8341 tgaccgtttt tctgacaaag atcttgttcc cttgttcccc ttttttgata tagattcctt
     8401 cttttttcaa cgcagcacga cctaaacaag ccgttgcacc tggaaattgt tcctcaatca
     8461 ataattgatt tctaaaggat cgttctggaa aacgagacat aaaattcaaa agttctttca
     8521 cgtcttcagg agaatgggta tgctttttca ccgcatccag tgcctgttgc gaaagggatt
     8581 tgacttcttg cttaatctct tctgccgttc gtttttgata gcccttttgt tttgtttttg
     8641 tttctttcga ctgcatttgt tctttcgaaa caggttcatt tttgaccgtt tgtttttgtt
     8701 ccttttcagg tacttgttcg cacaattttg ctaactctaa atagatgggt tgattactgt
     8761 tgacgccatc ccctagatcc attcggatct gatcgactac ttctccgttc ttcacttctt
     8821 caaaatagaa tttataataa cctgcttctg cggcgtattg gttatccaac gttttgagtt
     8881 gatcgatcaa ttctaatgtt acgatctccc caccataact tttcaatcct ttttctggat
     8941 cattttcgtt gaattctact cgatagaaat gatcgttttc tttctccttc aaagaatcag
     9001 ttggcgcagc ctcttcaagt aattcacgaa cagctgcttt ttcctcttcc gacgtggctt
     9061 ggtatttttt tgattcctca aatcccccgt tttcattcag ttctaaagca acttttcgat
     9121 agtctttcaa aaaataaatg gttggatttt ttttccattg cgtcactcgt ccgctgtcaa
     9181 taattgcttg tacttcagga agaagatttt cttttccttt ttctgatctt tctttcagct
     9241 gttctaatgt ttccaatttc cgttggtatt ttcggatcgt agcggctgaa aaagtcgatt
     9301 cacctttttc aaagcgttcg atttcttctt tgattcttgg aatattttcg attgtcatat
     9361 ccaggccgcc gtacttgtta tagcccattt cctttagctc ttcttgtctt tcaagttttt
     9421 ctacctgttt ttcttgttgc ttgatctctt ctctcagact atgcactttg ttttctaaac
     9481 gttcttgttg cctaaaccaa gaatgcccat ttctcttatc gttcattggc tgaccgttgg
     9541 tctttctctg gtgttcaaac atcttctcaa tttgttcgtt taattcattt tgcaatcgta
     9601 ctaattttct tttggatttt cccaagcgac tattgtctag tggttctttt tcttctgcca
     9661 tgtcc
//
LOCUS       DACDBG010000094         9606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDBG010000094 DACDBG010000000
VERSION     DACDBG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.094"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..396
                     /locus_tag="IVZ90_002382"
     CDS             <1..396
                     /locus_tag="IVZ90_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8263386.1"
                     /translation="VLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNM
                     IKRNYKLPDWIEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTS
                     DASNNSNTNKTSTSSTESKTKENKKSSDE"
     gene            416..934
                     /gene="msrA"
                     /locus_tag="IVZ90_002383"
     CDS             416..934
                     /gene="msrA"
                     /locus_tag="IVZ90_002383"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8263387.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            931..1158
                     /locus_tag="IVZ90_002384"
     CDS             931..1158
                     /locus_tag="IVZ90_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8263388.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            1310..1861
                     /gene="lepB"
                     /locus_tag="IVZ90_002385"
     CDS             1310..1861
                     /gene="lepB"
                     /locus_tag="IVZ90_002385"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8263389.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1944..2486)
                     /locus_tag="IVZ90_002386"
     CDS             complement(1944..2486)
                     /locus_tag="IVZ90_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8263390.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(2723..3121)
                     /locus_tag="IVZ90_002387"
     CDS             complement(2723..3121)
                     /locus_tag="IVZ90_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8263391.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            3381..4241
                     /gene="ylqF"
                     /locus_tag="IVZ90_002388"
     CDS             3381..4241
                     /gene="ylqF"
                     /locus_tag="IVZ90_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8263392.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            4234..5001
                     /locus_tag="IVZ90_002389"
     CDS             4234..5001
                     /locus_tag="IVZ90_002389"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8263393.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            5057..5914
                     /gene="dprA"
                     /locus_tag="IVZ90_002390"
     CDS             5057..5914
                     /gene="dprA"
                     /locus_tag="IVZ90_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8263394.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            6036..8114
                     /gene="topA"
                     /locus_tag="IVZ90_002391"
     CDS             6036..8114
                     /gene="topA"
                     /locus_tag="IVZ90_002391"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8263395.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            8083..9459
                     /gene="trmFO"
                     /locus_tag="IVZ90_002392"
     CDS             8083..9459
                     /gene="trmFO"
                     /locus_tag="IVZ90_002392"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8263396.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         3349 a         1684 c         2002 g         2571 t
ORIGIN      
        1 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
       61 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
      121 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
      181 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
      241 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
      301 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
      361 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
      421 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
      481 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
      541 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
      601 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
      661 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
      721 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
      781 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
      841 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
      901 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
      961 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
     1021 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
     1081 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
     1141 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
     1201 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
     1261 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
     1321 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
     1381 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
     1441 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
     1501 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
     1561 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
     1621 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
     1681 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
     1741 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
     1801 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
     1861 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
     1921 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
     1981 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
     2041 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
     2101 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
     2161 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
     2221 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
     2281 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
     2341 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
     2401 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
     2461 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
     2521 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
     2581 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
     2641 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
     2701 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
     2761 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
     2821 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcgtttt
     2881 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
     2941 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
     3001 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
     3061 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
     3121 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
     3181 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
     3241 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
     3301 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
     3361 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
     3421 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
     3481 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
     3541 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
     3601 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
     3661 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
     3721 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
     3781 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
     3841 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
     3901 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
     3961 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
     4021 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
     4081 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
     4141 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
     4201 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
     4261 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
     4321 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
     4381 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
     4441 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
     4501 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
     4561 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
     4621 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
     4681 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
     4741 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
     4801 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
     4861 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
     4921 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
     4981 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
     5041 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
     5101 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
     5161 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacatc gggagctgtt
     5221 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
     5281 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
     5341 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
     5401 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
     5461 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
     5521 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
     5581 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
     5641 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
     5701 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
     5761 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
     5821 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
     5881 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
     5941 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
     6001 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
     6061 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
     6121 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
     6181 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
     6241 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
     6301 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
     6361 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
     6421 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
     6481 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
     6541 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
     6601 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
     6661 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
     6721 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
     6781 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
     6841 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
     6901 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
     6961 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
     7021 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
     7081 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
     7141 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
     7201 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
     7261 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
     7321 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
     7381 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
     7441 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
     7501 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
     7561 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
     7621 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
     7681 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
     7741 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
     7801 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
     7861 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
     7921 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
     7981 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
     8041 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
     8101 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
     8161 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
     8221 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
     8281 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
     8341 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
     8401 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
     8461 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
     8521 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
     8581 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
     8641 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
     8701 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
     8761 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
     8821 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
     8881 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
     8941 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
     9001 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
     9061 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
     9121 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
     9181 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
     9241 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
     9301 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
     9361 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
     9421 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
     9481 agacggatag tctcgctctt tttctggctc tttgtcaata aggactgatg agttgtgcaa
     9541 aatcaaatct gagtcagaat gaaccacatt ctggctcaga ttttttgtta tcttacattg
     9601 attaat
//
LOCUS       DACDBG010000095         9563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDBG010000095 DACDBG010000000
VERSION     DACDBG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.095"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..717
                     /locus_tag="IVZ90_002393"
     CDS             79..717
                     /locus_tag="IVZ90_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8263397.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(1041..1124)
                     /locus_tag="IVZ90_002394"
     tRNA            complement(1041..1124)
                     /locus_tag="IVZ90_002394"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Leu,seq:cag)
     gene            1652..2152
                     /locus_tag="IVZ90_002395"
     CDS             1652..2152
                     /locus_tag="IVZ90_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263398.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2342..2686)
                     /locus_tag="IVZ90_002396"
     CDS             complement(2342..2686)
                     /locus_tag="IVZ90_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8263399.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2840..3760)
                     /locus_tag="IVZ90_002397"
     CDS             complement(2840..3760)
                     /locus_tag="IVZ90_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8263400.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3947..4438
                     /locus_tag="IVZ90_002398"
     CDS             3947..4438
                     /locus_tag="IVZ90_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8263401.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4462..6837)
                     /locus_tag="IVZ90_002399"
     CDS             complement(4462..6837)
                     /locus_tag="IVZ90_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8263402.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            7163..7789
                     /locus_tag="IVZ90_002400"
     CDS             7163..7789
                     /locus_tag="IVZ90_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8263403.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7812..8516
                     /locus_tag="IVZ90_002401"
     CDS             7812..8516
                     /locus_tag="IVZ90_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8263404.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8708..9541
                     /locus_tag="IVZ90_002402"
     CDS             8708..9541
                     /locus_tag="IVZ90_002402"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8263405.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2917 a         1848 c         1696 g         3102 t
ORIGIN      
        1 tgaaatattt tcacgtgtta aagtattcct cgttgaacac aacaaataaa actttttaac
       61 gaataaagga gatttttcat gacatcactt aaaactttac tttttggaac aactttggct
      121 gccggcgctg cattcttcat gggaacgact gctcatgcgg acgaagctta taccgttcaa
      181 tcaggtgata ctctatcaac gatttctcaa aaatacgttg gtgataactc cttgatcaat
      241 gccattgcag aatcaaattc aatttctgat attaacctga tctactcagg acaacaatta
      301 acaatcccaa cagaaggctc tgcacaagca gctgctgaac ctcaagcagc cgttcaagaa
      361 gcaccagtcc aagctgaacc tgttcaagct gaacaacctg ttgtacaaga aacggttcaa
      421 acggaaacac aagcagcgcc tgttgctgaa acacaacctg ctcctgctgt aacagaaact
      481 gctgctactc cagcaagtac aagctctgca aaagaatgga tcgcacaaaa agaatcaagc
      541 ggttcttaca cagcaacgaa cggacgttac atcggccgtt accaattaga ttcttcttac
      601 ttaaatggtg actactctgc tgcaaaccaa gaaagagtag cagaacaata tgttacttca
      661 cgttatggtt cttgggaagc ggcgaaagca ttctgggaag caaacggttg gtactaagat
      721 tcattatttg aacgtttaaa cacattcaaa atatagtaag aggtcatgcg atttattctt
      781 tcgcatgacc tctttttgag tttaagttta gtctttattc cttaactatc gtttgccagt
      841 atttcttgta tcagaatcta taaatctact atttgaatca atctattagg ttttaaactg
      901 tcccaccttc acacaaattt tttttgattt atttgaatct ggttccgttg tttaaaattt
      961 ttcctatatt cttttagata atatgttatt ttctttctca tcgctccagg tgtaccttct
     1021 ctttataaaa aaacaaatta tgcggctaag aagactcgaa cttccacgag gtcaccctca
     1081 ctagcgcctg aagctagcgc gtctgccaat tccgccatag ccgcatcaca tcttttagtt
     1141 taggattaca tgtgtttttt gtcaagaaca tatttttgtt ctttttcatt tctgtacgcc
     1201 ctcccttatg ccatctacac aaataattca tccgagcaac tccatcatct attgagggtt
     1261 ggtgaaaaaa tctgttttat ccatatgatc gatttcaatg ttaaacgaac gactctggga
     1321 ttagtaacta ttttattttt ttcttcattt cttttttcct ctttttctcg attcagctat
     1381 cttctttcat ttggatattg attatttctt ctgttgatta ctatccttct ggaattctag
     1441 acgctgaagt aattcacgtt ttctttcttc tagagatcat aagtaacgaa tgaacgaact
     1501 tcaaataatt tttttattgt aattcatatt tgtattgttc gatgattctt aatacgatat
     1561 gacacaataa ataaaaatag gcatggctat ttacaacaat agtgctatag tagaaaacaa
     1621 atgaataagg ttacttaaag aggtgaatac gatgaaactg atcaaactat gggaagcaga
     1681 tttaaagaaa gcttatcagc tacaaactag ctttggacca aatgagagtg gatttatcaa
     1741 caatgcttat ggctatacat ttgctgaatt tttagattat gtaaaaaaat gcgaaaaaac
     1801 gtctttagga atcgatcttc ctgagagtta cgttcccgca accgtcttta ttttagaaag
     1861 tgatgaacat gagtatgtag gaatttttaa tttgcgccat aaactgaacg aaacattaag
     1921 aaacggcgct ggccatattg gttacggcat ctcccctaat tatcgtaaaa aaggatatgc
     1981 cacacaagga ttagcattag tactacaaga ggcaaaaaaa atcggcattg atgaggcact
     2041 tttgtctgta aataaaaaaa atcttggcag tcttaaagca caacaaaaaa atggggctgc
     2101 gatttatcgt gaagatgaga aagaatacta tacgaaaatc aaactgatgt aaaaaagaag
     2161 gattccaaaa ggaaagggat ttgaatgtcc tttatcaata gaacacattt atttaattaa
     2221 cttataatat agcagacaaa attttataaa caaagaaacc ttttatctct tttcttctag
     2281 aaaggataaa aggctttttt gtttttccta tttgcttctc atagctagtc agccaagatt
     2341 tctatcgtat ccccaacgca atccgtgcat aacggctcat cttatctgtc gtccatgctg
     2401 gataccagac tagtttgact tctgctttcg ttacttctgg cacttctttt aatgcgtcat
     2461 ggatagattc tgttaaaata tctgctaatg gacagcccat agttgttaga gtcatcttaa
     2521 tgactgtttc tccattttca ggattgaatt ctatatcata aatcaagcct aaattgacga
     2581 tgtcgattcc taattcagga tcaatcacca tttccaaggc agcaagaata cgttctttga
     2641 ttagttctat ttcttgtcca gaccgttctt ggtttgcttc gctcatcgta cgacactcct
     2701 taacacgatt tttatttatc atactctttt tttagctgct tgtaaatgag aatttagaag
     2761 taagattctc gtttaaattt ataaaaatgt tcttattaga tgtaatagat taaatttatt
     2821 ctaaaataaa cctgattcct catttttttt gtgaacgatt ttttatttct ggcaatacgc
     2881 cagttgttcg atacattttt tgttcgtttt catttgcaaa ccggccaaat actgaatgag
     2941 ttggtaaaat attgataaat gcatcgtcgt cgatttcatg aacgatctga tcaagatcat
     3001 acatttcgta gcgggtgatg accatcatga tcatgttccc ttgttttttt gaatagccac
     3061 cgacagacgg cagtaatgtc attccacgta ccatccgttt tgctagttct tctgttacct
     3121 cttctggctt gacggtaacg atcattgctg ttactttttg atgactcgtg tggatggcat
     3181 caattacttg agtcattgca taaatggaaa taatcgtata caaggcactt tcccagttaa
     3241 agaagaagcc tgcgatgact acaatgatac catttagaat cagcatgtag tttccgactg
     3301 tttttccagt tgtttgtgaa agtacaagag agatgatatc catcccacca gttgtaaagc
     3361 ccatttttaa agataatcct acgccaagac ctaataaaac accgcctact agggcattca
     3421 tcaaaatatt atcagtgatc tgtccgactg gaagcagcat cgtcatcacc gatacacata
     3481 caacattcac aaaactaagg atcgttgctc gttttcctac tttgagaaac cctaatacaa
     3541 acacagggat attcaaaaga aaaataaatg ttcctgtgtc tatcttgata tgaaaccatt
     3601 catataaaag cgaggaaatg atttgagcga tcccattcat ccctgcagaa aagacattgg
     3661 ctggaatcaa aaaataattt aaagcgaccg cagcggtcaa tccggttatc aaaataacca
     3721 ctattttctt taatctttca tttttatgat acgcagtcat aattttactc acaagcacct
     3781 ctcccctact ttggtattcc aaatgtaaga ataacagttt atctattaaa ataaaatact
     3841 tcgaactcaa atgagaagaa ttttagcaaa caggtaagaa tctgccctaa tcctatgata
     3901 agatagatgt atggtaaata tttatatgta tatcaggagg gttcctatgg caaaagatgc
     3961 tagcttcgac attgtatcag aagtcaatac agaagaagta aaaaatgcga ttcagcaaac
     4021 aacaaaagaa ttgtccaatc gctttgactt caaggattct acagtagaaa tcaaattaga
     4081 gaacagcaat ctagttgtat tgagcgatga tgatttcaaa atcgaacaga tcaaagatgt
     4141 attatttggt aagctgatca aaagaaatgt gccaatcaaa aatatccatt tttccgaatc
     4201 agaacacgct ctaggtggca aagctcgaca aactgccgat ttggtcaacg gaatcgatcg
     4261 tgaaaacgcg aagaaaatca ctacagctat caaaaatgaa aaactgaaag taaaagcaca
     4321 aatccaagaa gatcagatta gagtgacagg taagaaccgt gatgatctac aagctgtgat
     4381 tgctttatta cgtaaacttg acttaccaat cgagttgcaa ttcactaact atcgttaatc
     4441 aaaaaacgag gctgacgtct gtcattcctc gtttttttga tttactgctt cttttgataa
     4501 ccaaaaacgc tgtattcctt cttccaattc cccaacactt cgtacttctc ttgctgtttg
     4561 ccaatgttgt gggaataaac gtcgataaga aaaactagaa aatcgttgat ctgccaatac
     4621 aacgattcct tgatccgcca tgccacgaat cactcgacca ccagcttgca agactttgtt
     4681 cattccagga agctgataag cgtaagcaaa tccaaggtgt tccttctcat cataatatga
     4741 tttgatcagt tcctgctcat gattcatctg cggcaaacct acacctacga tcatactgcc
     4801 gatcaatcgg tcccctctca aatcgattcc ttctgaaaaa atcccgccaa gtacgcaaaa
     4861 tccaatcaat gtttcagcgg gatttgtttg aaattttgtt aaaaatcgtt cgcgttcttc
     4921 ctcattcatg tccgtttctt ggattagcac attgctgtcg ggatattgct gtctgaatgc
     4981 ttccaccgca agatctaaat attgataaga aggaaagaaa acgaaataat tcccgacctt
     5041 tccatcagca aaagcatgaa tcgtctctac taattttgcc agactgtgtt ccctttttcg
     5101 ataagtcgtc tctaggtaat ttgcgatgaa aacttgctgg tttccttctg ggaaaggact
     5161 cggcagtttg taagccagac ttttttgtcc ccccagtgtt tcctgataat aagagagtgg
     5221 tgaaaaacta gcagaaaaaa gaatactgct ttttccttta tccaacgact gctcaagaaa
     5281 caagcttgga tcaatacaaa actcttttac tatcagatcg cgatacgttt tttcgactgt
     5341 tgtttcgtag tgatcatcat aaaattcact gaccttcaaa aaatgaagca aatcaaaata
     5401 ataaggaagc aattgttctt gtgatgggtg ctcaggaaat tctgccagcc attctttaat
     5461 tttttcgctt aattgataac ctgcctttac taaagattca gccggtgctt tttgatgatg
     5521 ataatcccaa tgatcttctt ttgctatttc tcggatcgaa tcaaattctt ttaatagttt
     5581 gttgaaacgg cgatgaagtt ttttgaattc ctttggtata gcttccttac ttcgtttcgt
     5641 tttttcataa gatagttctg ccgaatacat ctctcgggaa cgattcacca aattatgtgc
     5701 ttcgtcaatc aaaaacagat agtcctcgtt tttttcatct tcaaaaaaac gtcttaaata
     5761 aacagttgga tcaaataaat aattgtaatc cccaatgatt acgtcacacc agatactgac
     5821 atctaatgat agttcgaaag gacaaagtgt atgctttctt gcataagttt cgatgacttc
     5881 acgagtgatc tgattctcat gattcaaaag atcccataaa ccttcattaa tccgattgta
     5941 ataaccattg gcatatgggc actgatctgg attacaggtc gtctcatcta aaaagcagat
     6001 cttgtctttc gccgtcaaag tgacactttt tacttcagct cctgtatctt ttaatgctgt
     6061 catagcatct tcagctactt gtctagttat tgttttagcc gttagataaa agatcttttc
     6121 tccctcttct tcccctacag ctttcaaagc ggggaataac gtagagattg tttttcctgt
     6181 tccggtaggt gcttctacaa acaatttctg tttcgtacgg atcgttttat aagcagctac
     6241 cgctagttca cgctgacctt tcctatactc atcatggggg aatttcaaag ccatcaaaga
     6301 ggcattgcgt acagtgcgcc agttttcttg aaaaacaagc cattctttat aatcttcagt
     6361 cagcttttta aaaaaagttt ctaactgctc tcttgtctga tgttcaacct ttctcgtgat
     6421 cacttcttct gccgtctgga agtaagtcaa ttgcagattt atttcttgta aatcatgttc
     6481 ttgactatag atataggcat acacccttgc ttgatgaaag aaaagttcca cttgttctgg
     6541 ttctaaatct tcaaatcgtg gttcggaggt tttgatttca tctatataat aaataccgtc
     6601 tcttttgaaa ataccgtctg cacggccttc aacgatcagg taatattcat ctaattgaac
     6661 tgttgttttt aaaaagactt ctttttcata gttttctccc gcatcttttt ggagcttgcg
     6721 gtggattctc gctccttcta atgcagtatg gttacttttt ttacgattgt cgatacttcc
     6781 acgtcgcaaa ataaattcta ctaagtgccg cacagatatt tttgactgtt tgatcatttc
     6841 caccacctct ttctctctaa gattacctga aaaaacctag tttagcaaaa agagctctct
     6901 ttccaaacat aaaaaaacac cctgctgatt aggcagggat ataggatata gaatttggat
     6961 ttttggatga atctaatgta acaaggacat attaaaatgc tatgaaaact tactttcctt
     7021 ctctttagaa tatccaaata cttttcatat agtctgataa ttcagataag taaagagaaa
     7081 taagtacctg tttttgtttc ctagcaatga atgcgttata ctaatggatg aaagaagttt
     7141 tggctatcaa ggaggaatgc acatgcatat cgaaagatta aaaacaggaa tgattgaaga
     7201 aaactgttat ttggtctaca acgaagaagc acttttgatc attgaccctg gagaagatgc
     7261 ggaacgaatc aaacaagaaa tcaaaaaaac aaaccaacag ccggtagcga ttttgcttac
     7321 ccatactcac tacgatcata tcggtgcagt ggaaccttta cgcgcgtatt atcaaatccc
     7381 agtttatgtc agcccactag agcaaaaatg gctaggtgat ccgattttga atctttctgg
     7441 actcggtaga catgatgata ttgctgatgt tattgtctcg cctgcagaat atgaatttga
     7501 aatgaaaact tatactttag gaaacatgtc ttttcgcgtc gtccctaccc cagggcattc
     7561 tataggaagc gtcagcttta tttttgatga ttttgtcgtt tctggcgacg ccctgtttag
     7621 aggaagtatc ggtagaaccg atctgtatac agggaattta gaacaattac ttcatagtat
     7681 tcggacccag ctgtttgtat tacctgatga atttgcagtc tatccaggac atggcgatgc
     7741 aacgacgatt gaacaggaaa aaaggacaaa tccttttttc aatcgttaaa acgacttagg
     7801 aggagaataa aatgacagaa actacacttt atatcgtacg acacggaaaa acgatgttca
     7861 atacaattga acgagtacaa ggctggtgtg atacacccct cacaaagcaa ggacaagaag
     7921 gcattcatta tttaggtcgc gggttaaaaa atgtcgactt ttcatttgct tattctagtg
     7981 acagtggtcg tgcaatcgaa actgcccgta ttattctagg agaacattct aagggaaaag
     8041 aaattcctta ttttattgaa aaaaggatta gagaatggtg tttcgggtct cttgaaggtg
     8101 gatatgacat ggaaatgtgg ggtgtgatcc ctcgtgtatt gaattttaaa acatatgatg
     8161 aaatgtttac cacagaagtt tcttttgaac aaattgctaa tgctgtatac aacttagata
     8221 ccgctgggtg ggcggaaact tatgaaacat tgcgagaccg tgtatggaca ggatttgaag
     8281 atatcgccca tcactgcgaa aaaaatggtg gtggaaatgt tttagtcgta tctcatggtt
     8341 tgacgatcgc ctttttgctt aatctgatcg atcctaaaca gcctgttcgt gctgggcttt
     8401 taaatggcag cgtaacaaag gtcacttatg caaacggtcg attttctatc cacggcatca
     8461 atgatacgtc ttatatcgat aacgggaaaa cacatagtcg cgactcgttt ctataatggg
     8521 gatattttaa tttttaccag agaatcataa aaggcaaagt ctgaacgagg gctttgtctt
     8581 cttttttctc ttcgtgtact tatctgcttt atttctcaag tttttcttaa tattaaaaaa
     8641 cagattgggt ttctcaaaaa ataacggtat actataagat gtatcttaat tgggaaggaa
     8701 ggaatttatg ttttttgcaa atattatcaa agcagtgatt ctcggtatta ttgaaggaat
     8761 aacggaatgg ctgcccatca gtagtactgg tcatttgatc ctagcagatg aattcatcaa
     8821 actggatgcc agttcacaat ttatgtcgat gttcaatgtc gttatccaat taggggcgat
     8881 cttggctgtc gtcgttcttt attttcacaa gttaaaccca ttttctccaa gtaaatcaac
     8941 ggtagaaaaa aaagatacgt ggacactttg gtttaaagtg atcgttgctt gcataccagc
     9001 catcatcatt ggtttgcctt tggatgattg gttagaagca aatttccaca agttcttacc
     9061 agttgccatt gttttgatcg tttacggtat tgcctttatt attgtcgaaa aacgcaacaa
     9121 aaacaaaaca cctcgttgga cttctctgaa tgactttacg taccaagcag ctcttcttgt
     9181 tgggttattc caagtcttgg cacttgttcc cggaacatcc cgttctggcg caacgatcct
     9241 aggatctatc ttgattggtg cttcgcgttt tgtcgctaca gagttttcat ttttcttagg
     9301 gattcctgtc atgtttggcg caagcggctt gaagatcgtc aaatatttag ctgaagggaa
     9361 ttcattcaaa atggaagaaa ctgttgttct tcttgtagga acattcgtat cttttgtggt
     9421 ttccgtctta gctatcaaat tcttactaag ctatttgaag aaaaacgact ttaccgcttt
     9481 tggctggtat cgtattatcc ttggtatcat tttgattggt tattggttta ttgcaatgta
     9541 gaaaaaacca gaacacttct ctt
//
LOCUS       DACDBG010000096         9399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDBG010000096 DACDBG010000000
VERSION     DACDBG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.096"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1034)
                     /locus_tag="IVZ90_002403"
     CDS             complement(<1..1034)
                     /locus_tag="IVZ90_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8263406.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDA"
     gene            complement(1047..1841)
                     /locus_tag="IVZ90_002404"
     CDS             complement(1047..1841)
                     /locus_tag="IVZ90_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8263407.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(1852..3228)
                     /locus_tag="IVZ90_002405"
     CDS             complement(1852..3228)
                     /locus_tag="IVZ90_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8263408.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(3357..4004)
                     /locus_tag="IVZ90_002406"
     CDS             complement(3357..4004)
                     /locus_tag="IVZ90_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8263409.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(4088..5515)
                     /gene="uxaC"
                     /locus_tag="IVZ90_002407"
     CDS             complement(4088..5515)
                     /gene="uxaC"
                     /locus_tag="IVZ90_002407"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8263410.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(5589..6668)
                     /gene="uxuA"
                     /locus_tag="IVZ90_002408"
     CDS             complement(5589..6668)
                     /gene="uxuA"
                     /locus_tag="IVZ90_002408"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8263411.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(6671..8302)
                     /locus_tag="IVZ90_002409"
     CDS             complement(6671..8302)
                     /locus_tag="IVZ90_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8263412.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            8619..9290
                     /locus_tag="IVZ90_002410"
     CDS             8619..9290
                     /locus_tag="IVZ90_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8263413.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         3026 a         1874 c         1249 g         3250 t
ORIGIN      
        1 aatgcatcac gtaaacgctc ttctgaaata attcctttgt aataaccttc catcatataa
       61 ttaaagtctt catctggatc attaaaaaat aggaatagat cacatccagc cgcaatagaa
      121 gtgggcaata attctgaccg tttcattgcc ccagtcattc ctaacatatg actagcatca
      181 gtaactacta atccattaaa cccgagtttt cctctaagta agtcagtgat taattctttt
      241 gataaagttg caggaagtaa atcttcatct ttcagatcaa gattaaaatg ttttgaataa
      301 gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat taagcctgag
      361 tatacttttc caaaagaatt atcccattct tctgtactta aactattaat actaaaagat
      421 agatgttgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc aggcaaaatt
      481 ccactctctt gaataccttt catataagct aaactaagtt ctaaaacttt atcagggctt
      541 tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat atctacaata
      601 ggagcaaaac tccagttaca tccaatagcc gatgcttcaa ttccagaaac tctccctaat
      661 tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc aacttcggta
      721 ccatctgtac aagctccgtt accacctgct tctgtattag cagcaattaa taaaggaatt
      781 ttgctttctg tttgtaggat atagttttga tcatatacct catcagcagt tcctggatta
      841 tatcgtacag ctccaatatg gtactgattg accatactac ttaagtaatt tttatcacga
      901 ctagatccca tattaacaaa taattgacct attttttctt ctaaagtcat agcatcaatc
      961 gtctcttcaa tccaagaaat agcatcttca tctaaatgat atggtttttt tcgtaagtct
     1021 acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca aaaagtatct
     1081 atatattttt gttcaatatc tatatactct cccgataaac atttaaataa aatatcagtt
     1141 cttaagtttt cccatgattc tttttcttta ttaaccaaaa taggataaaa gaaaacttta
     1201 ttcttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat cttatccaaa
     1261 tcacaaccat tttcaattac acttcgaata atgtcttctt tttttccttt atcttgacaa
     1321 cacagttcat caactaagga taataattca tgtctttccc attcttcttg aatagcttcc
     1381 ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt tatagtatct
     1441 ctagcactta aaaagggttt atctaatcct ttagcttgac taatctgtcg attgacttct
     1501 tccgaccatt ttaaagcttt taataaatcc tctgactgag agtattctat ttccctcttc
     1561 aaagatggat tagacaattc ttttgttaca gatacccagt gctttaatcg attaatcgat
     1621 ggtacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat tccacgagtc
     1681 tctgaatata aattaatatt attccaatcc tgtaggaatt tttccctatg atgaacatca
     1741 aaaatctctg cagcaattgg cccgaaaaag agattgtgtt tataagtcat tgtatccatg
     1801 acacaaccat ctgaatcaat acaaaatatc caactgttca tgttttttct cctagttatg
     1861 tttttgattt agcaaattat aattttctaa tactttttct ttagataatg gatatacgaa
     1921 aaataaaatc aatcctacaa caaagaaaca aactgctgga atcaacgttg aagagttata
     1981 cagcttttgt aaaacttctc cactttgtac ttttgctaac gaatcatacc ctattataga
     2041 aagagtccaa cctccgacac cgccagctaa agcttgtcca atttttcggg caaaagaata
     2101 tactgcataa atagttccat cttctctttg gccagatttc acttcttgat catcgattac
     2161 atcagtaata ttcgcccaaa tgactgtatt aaaaatacct aaccccatat atccaacaga
     2221 agtaagaact aagaatacat acatattact aggctttatc aaaaataaaa gtaaatatac
     2281 aatagcagag aagaacatcc ctactgacgc caattctttt ttaccgtatt ttttactgaa
     2341 aattaaactt acaggtgaaa caagtaccaa catcaaaata ggattgataa agttcattaa
     2401 aactattccc tgtgcattat tataataatc tggaaataca taattgttca tactcattgt
     2461 taaaagctga gcaagcaaca aaaaaatagc tgcagctatt atagaaagca acgctctatt
     2521 tttagctaat ccttttaaag atttcaacaa tgaaactttt aactgcgatt catcactttt
     2581 aacccgttct acagttaatt tataacatat tacgtaacaa ataactgcac acactgaaaa
     2641 tactcctgcc acaattgtaa aagttgatcc accacgtaca atttgagctc cagaactatc
     2701 tcttgtataa ataaaaattg gtgttaacac gccaatgaac atacctgcta aggtagctcc
     2761 tatactacga aatgttgata acgctgtcct ttcttctgca acagaagtga tagctgatgc
     2821 cattgaaccg taaggaatat taatagatgt ataaaaaata cttccccata gtatatatgt
     2881 tacatacata tatataactt taatactcat agaagctgat gccattgatg tttgatacat
     2941 taaaaaactt gctaaagcga ctggaccact catccgtaaa atccacggtt taaatttgcc
     3001 agttttagat ggttttgatc catccacaat acgccccatt gttatgtctg taactgcatc
     3061 aataaatctt gctactaaaa ataatgttcc tacggtagct ccgcttatcc ccaatacttt
     3121 tgtataaaaa atcattaaaa aagaactagc aaaaatgaaa gtgaaatcat tcccaaaatc
     3181 tccaaacata tatcctattt tgtctcttag accaaatttt ctttccatat aatttgcgct
     3241 ccttcctatt gttaagcgtt ttcattctgt tgaactgcca acttacgtac tatactctga
     3301 agtataaaga gttagtattt attttttaca ttttatagta ttatttattt tttctcctaa
     3361 taattcttta tctcataaaa cttatctata tatttactag ctattcgagt tacttcttca
     3421 aaatcacctt tttcaacagg agccaacaaa ttcccaccta caccaactgc aacaacgcct
     3481 gcttcaaacc actcttctaa gttatctaga cttactccgc cagtaggcat tatatttaca
     3541 tatggtaatg gagcttgata cgtttttaca acagatggac caaaagcaga acctggaaat
     3601 agtttaatga tttctactcc agcggtaagt gcctccttta tctcagtaat tgtcatacaa
     3661 cctggtaaat aaggaatttg atataaatta caaatctctg ctgtttgtcg atcaaaagat
     3721 ggacttacga tatattctgc tccagccata attgcaatac gagcagtaaa cgcatctaaa
     3781 actgtaccgg ctccaattat tacatctttt tgatcagcat attttttagc aatttctttg
     3841 attatttctt cagcattcgg aactgtaaaa gttagttcaa tccctttaat tccacccgca
     3901 gtaattgcat ttattgcttt aatggcctca tctttatttt ttccacgtac aaccgcaatg
     3961 actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcttt
     4021 tatggataca tactctagct agttgtacta acttacaata aataaattta agcttttatt
     4081 ttttgtatta ttttctactt tcgatttcaa aaaaattaaa ataattatac gcattacgat
     4141 aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt cgacctgctt
     4201 taacccactc gcttagtaat tgacataata ctcgacggaa atactcatgg cgaggatagc
     4261 ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgcg
     4321 ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc
     4381 ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa
     4441 gccaatcggt tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag
     4501 cagtgtcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac
     4561 caccgacatc tactccaatt tgttccttta atatacttga attatttctt aaagcattta
     4621 aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac
     4681 ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta
     4741 ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt
     4801 gatctgctaa tcgacagcca atttgatgaa aataatccac tcgttggctc aatgcctgct
     4861 tcaacccatt aaagtttttg attggtatac ccgctgcttg actaagtttt aaaatatagt
     4921 tcgaaaatcc tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct
     4981 tgaaagaatt atcctgttta gaaagctcag tatgatatcg caagtcatct gtaggatcat
     5041 ccgttgtaca aatggctttc acatccatca tcttcagcaa ttcttttggg cgataggaat
     5101 ccgtttggat tttttcgttg gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg
     5161 tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac
     5221 ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca ttacctgcac
     5281 cactgatcaa ctcttcttct atcccattag ctcgcatcaa tcgccattta taatgatctc
     5341 cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca
     5401 aacatgctgg gtctaaatga caatgataat cgatgattgg ttggtgttct gcaaatttat
     5461 gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta
     5521 agcactcctt tccttccctt agtttttaga agagaaaatt tttcttctaa atagactcca
     5581 ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact
     5641 gcacctagag ctcggtcata taacccataa ccaggtctac cggtttctcc ccaaatcatc
     5701 cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc
     5761 ttatacatat ctagagaacc atattctgat ttatgcggcg cttcataaaa atcgagtcca
     5821 ttatcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa
     5881 aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat
     5941 aacgtaatac cattagaagg agaatccact acattacaca accattctaa atcgtcacga
     6001 tttttaacaa tacgaggtaa tccgaaaata gaatatggtg gatcatctgg atgaactgcc
     6061 attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata tgcaaagttc
     6121 tctcgcaatt tcgcttcatc aacttcttta taggcttcta ataattgctt gatttgtgat
     6181 aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc tacttttcta
     6241 ataatttcct ctggatcagt aggaatattt tttgaaatat aggctaacgt agaagaccca
     6301 tcatctaaat gataatccaa atctgatttt acccaatcaa agactggcat aaaattataa
     6361 cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcgatg
     6421 tattgatctc gtgttggcaa accaattttg atatcatcat gaacattaac actctcaata
     6481 actttcaagg taaggccatt ggcttcgact tcatctttta aagctttaat tcgttctctc
     6541 ggccagacct ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg
     6601 atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc
     6661 atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg
     6721 cttgaggacc tactagcatt tcgcaaaaga ttttttcaat tttctttcct atctcaactt
     6781 gatataaatc taaaccaaat atttccttat tttctaagat ttcctgtacc gcacaatgaa
     6841 tctttgcagg atcttgttca cctaatttca atactgagaa tttttcctgc aactctacca
     6901 ataaaggatc aggacttggc acaaaatttt ttccttgatc atcgactgcc aataaataac
     6961 gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt
     7021 cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg
     7081 caattcgttg cggtgtatct ggaatatagg gattaggcaa acgtttattg attacttcat
     7141 caataaattg ttttggatca ataacgcctg gactttcaac tactggtaag ccttctacat
     7201 aacctatccc tttaattaag ccaaccaaat catcatcttg catttctgcc tgaattgatt
     7261 tataccccaa caaacaacct gttattgcta atgccgtatg gagcggattt aaacatgtcg
     7321 taaccttcat gatatctgct ttatcaactg tttctctatc tgtcataatc acaccagccg
     7381 cttccagttt aggtcgacca tttggaaaac tatcttcaat gactagataa tggacttttt
     7441 cagtattggt aaaagcagcg atattggtat ttttcgcagt atgaattata tctaagtctt
     7501 cgaaacccgc cttcaataaa tccattttga cactttccga aggatttggc gtaattctat
     7561 caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt
     7621 cagaaacgta tccttttttt tgccaagctt ctgcaatcat aaaaatgcta tcttgaaaac
     7681 gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggtaac tctccagcat
     7741 taaacctgct atacaacaag gatgtcacaa tactcatcgt atgttgagga ttggtaggtc
     7801 cagattttat atctgcttcc actaccgaaa aaaattgtcc tttagaatct tttaaactgt
     7861 atcctttttc agtaatagta aacgttgtaa actgtaatgt aggatcttca aaatatttaa
     7921 ccactctttg atagtccgtt atattctttg gattgcaata taaactatca gctgttactg
     7981 ctaaaagtct ttttttgagt tcaccatctt cacccataac aatctccaaa atatcattgt
     8041 tgtagtcatg ataagctttt tctactactt cttcatcaaa tgtctcacac acaactactc
     8101 cggattctaa ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat
     8161 aaagatttcc tccaccaaaa tgaagccaca ctggatgttt ctgaccttct tgtttgaccg
     8221 cttcgacatt aaaatacggt agttgaactt gcattttgtt caatccttcg atagaatctt
     8281 gccagtcttt aatcaaaacc atatttaccc ctccatactg atatgttagt ttccgttaca
     8341 attgtagtat aaaatcgaat gtaatcgctg tcaataagaa aaaactaaaa atattctttt
     8401 ttctaccatg tcagaattat tgtgtctttt tttagtaaaa gcacaccaat atatattttt
     8461 tagaactaac ttaaattaaa gcttaatttc tctttgatct tatttgagta ttttttgttt
     8521 ttttaatagc tagtatgtta ctttgaaatt tgatattata taacgagcat agaactaaaa
     8581 tatcaaattc aaaaatgaaa ttttgagagg gattaaatat gacaattaaa actagtctcc
     8641 aaaatcaagc ttatcaatct attcgtcagc aaattatcta cgctgactta gaacctggaa
     8701 aaaaagtatc agaaaaaaaa ttggaagaaa cgctttctat cggtcgtact ccaatacgtg
     8761 aagcattgat tcaactccgt catcaaggac ttgttgatac aattcctcaa agtggaaatt
     8821 atatctcatt aatcgactta gatttagcaa aaaatgctcg ttttgtcaga gaacatttag
     8881 aacgacaaat tatgctagag tgttgtgcca agatgaataa tcaaaagaaa caagttttag
     8941 aaactataat tgcagaacaa gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc
     9001 ataatgataa tttatttcat aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc
     9061 aatggttgga agataataat acgcacttgg aacgatttcg ttggttacga gtaaaaacgg
     9121 aaggattaaa atgggaaaaa attatggaac aacatcatca actatttaaa gccttgacca
     9181 tgcaagaccc tgaagaagct aacttcttaa catccttgca tcttcattta atgcttgatg
     9241 aacaaaatgt tgtattgact actcacccaa aatattttaa aaatatataa ctgctaaatt
     9301 tctttttttt aatttattta catttctgat tactacagaa aactaactac tttttttatg
     9361 taaagttctg tcaaatatag tcactttata ttactttct
//
LOCUS       DACDBG010000097         9359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDBG010000097 DACDBG010000000
VERSION     DACDBG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.097"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            440..739
                     /locus_tag="IVZ90_002411"
     CDS             440..739
                     /locus_tag="IVZ90_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263414.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(836..1270)
                     /locus_tag="IVZ90_002412"
     CDS             complement(836..1270)
                     /locus_tag="IVZ90_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263415.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1502..2212)
                     /locus_tag="IVZ90_002413"
     CDS             complement(1502..2212)
                     /locus_tag="IVZ90_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8263416.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2202..3062)
                     /locus_tag="IVZ90_002414"
     CDS             complement(2202..3062)
                     /locus_tag="IVZ90_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8263417.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3067..3705)
                     /locus_tag="IVZ90_002415"
     CDS             complement(3067..3705)
                     /locus_tag="IVZ90_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8263418.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3720..4019)
                     /locus_tag="IVZ90_002416"
     CDS             complement(3720..4019)
                     /locus_tag="IVZ90_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8263419.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4062..5546)
                     /locus_tag="IVZ90_002417"
     CDS             complement(4062..5546)
                     /locus_tag="IVZ90_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8263420.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5566..6027)
                     /locus_tag="IVZ90_002418"
     CDS             complement(5566..6027)
                     /locus_tag="IVZ90_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8263421.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6045..7112)
                     /gene="ulaG"
                     /locus_tag="IVZ90_002419"
     CDS             complement(6045..7112)
                     /gene="ulaG"
                     /locus_tag="IVZ90_002419"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8263422.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7330..8100
                     /locus_tag="IVZ90_002420"
     CDS             7330..8100
                     /locus_tag="IVZ90_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8263423.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8373..9200)
                     /locus_tag="IVZ90_002421"
     CDS             complement(8373..9200)
                     /locus_tag="IVZ90_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8263424.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2894 a         1928 c         1472 g         3065 t
ORIGIN      
        1 aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc tttacttggt
       61 tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa gaacgagcta
      121 gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta aatccaatgt
      181 atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta tagaattaga
      241 agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc atatgattat
      301 ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga atcaaatata
      361 cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat aatgtatcag
      421 tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac tacttaagca
      481 atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag tgacagagta
      541 tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa aaggaatata
      601 cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa
      661 tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt
      721 gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact aagtctatcc
      781 tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt atatgttatt
      841 tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa ataccaccca
      901 tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc tcagtatcat
      961 ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa gcttcattca
     1021 aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata tcttgaataa
     1081 atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc ccttccccat
     1141 tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca gtgtcttttg
     1201 tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc ataagggaat
     1261 caatattcat ttttttcttc ctttcttata caattctccc tctaataata cagttctttg
     1321 actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc ttaaaattcc
     1381 tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa attttcttca
     1441 caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta ccctagcatc
     1501 tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta tctagtaaat
     1561 attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc atcagacaaa
     1621 tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc catgtgaacg
     1681 gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt ctttccttaa
     1741 acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact tcttctttcg
     1801 ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc gtgccataag
     1861 ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa
     1921 tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga tctgaagatg
     1981 gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc atttgatctg
     2041 cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct agctcacggt
     2101 ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc aatgccagat
     2161 ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac atcatagcca
     2221 gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct tcgaaagtct
     2281 tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag acgtttcaaa
     2341 tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc acgaaactgg
     2401 cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt gatataatcg
     2461 atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa attgcctagg
     2521 tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa aaattttgaa
     2581 atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc atatttagaa
     2641 gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt cattgatttc
     2701 tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag attatgcgcc
     2761 aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg atcagcagaa
     2821 ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac accggagctg
     2881 aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg tgctaaccgc
     2941 tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt tacaagggaa
     3001 aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc aatttgagcc
     3061 atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg
     3121 atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga tcccttcaaa
     3181 taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc ctagttcaat
     3241 cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata acagcgcatc
     3301 acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca tggcttgttc
     3361 aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt gtgctgcctc
     3421 catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg cttttgcaac
     3481 attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta gttttttatc
     3541 tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt
     3601 gcccacttca ataatatcta ctacatcacc aactttcata acatctccta atgcatgttc
     3661 taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag ttccttcctt
     3721 tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc tttgactgac
     3781 atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt aaacatattc
     3841 aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt tccctctgaa
     3901 atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc tttttccaca
     3961 cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa aattctcatc
     4021 ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct ctgcttttct
     4081 gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt ccgatattgt
     4141 aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata acctaggact
     4201 ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc gatataacca
     4261 ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac ttgtatgatt
     4321 cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt ggcataaact
     4381 gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat cgttggtgat
     4441 ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa caagaaccca
     4501 atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat tcctggaaca
     4561 gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc atcgacgaat
     4621 gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga agtcgtcata
     4681 atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa ataatctttg
     4741 cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt agcaaccata
     4801 ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt tttgtcttgc
     4861 ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa ttttgcaaat
     4921 ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc
     4981 tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa aatcagtccc
     5041 ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa
     5101 gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg cagtttcgta
     5161 tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa taaaatcatc
     5221 acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc attttgtcca
     5281 aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt
     5341 ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta accgaccgtt
     5401 gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat atagcctaat
     5461 aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc
     5521 caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat tatattctgc
     5581 catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa ccaatccatc
     5641 tgtcattaat aattcggata atttttggat attttctaga tgttcttctg gatcttttgc
     5701 tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac tttcaaaacg
     5761 gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat cacttttttc
     5821 tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt atttttctac
     5881 acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga tcaatacaag
     5941 acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt aatgaatcaa
     6001 ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa aaacgacgga
     6061 aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa attaaactca
     6121 agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat ataaggttta
     6181 aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc ttttggatca
     6241 gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc attcaatgac
     6301 tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg tgggttttca
     6361 ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc aaataaattg
     6421 gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa taaatagttt
     6481 actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc tggatcttca
     6541 caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat gtctttgatt
     6601 ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc ccatttttgc
     6661 cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt ttgatgaact
     6721 gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat taaagcgtct
     6781 acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt aggctgcata
     6841 ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc attcccatga
     6901 ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc atgacttttc
     6961 agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc cactacttct
     7021 gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac taaccacttt
     7081 tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata tcgttgttcc
     7141 aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta tatgaccgtt
     7201 tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat aaagacagat
     7261 atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt ccctcaggag
     7321 gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga aattttggaa
     7381 ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct tgatgtatct
     7441 attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat catacgcaat
     7501 tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa
     7561 aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta tgatacgctg
     7621 tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc taaccatttg
     7681 acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa ttgtagttat
     7741 attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga catcgcatta
     7801 aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg agtcgatgta
     7861 gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat gatggtcaac
     7921 aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact gacatcaaag
     7981 ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt ttgttctgaa
     8041 aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga ttcattttaa
     8101 gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa atttatcgat
     8161 aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat ccatccaatc
     8221 gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa ataggaatca
     8281 aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg aataaacggt
     8341 attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc aatcaccttt
     8401 accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata tctttctaaa
     8461 taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa gtattccaat
     8521 gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa ttggattgct
     8581 ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac aatgcagacc
     8641 atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac ataaactttc
     8701 gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc tgcttgtaag
     8761 tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa taatgctgca
     8821 tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat ttgatttaaa
     8881 agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata agattcttgg
     8941 tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat tttgtcttct
     9001 tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag cgcacttaag
     9061 atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc ttgttcacaa
     9121 atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc ttttagtaat
     9181 gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt ctcctttata
     9241 tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata aaaattttaa
     9301 ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt ctttttgtg
//
LOCUS       DACDBG010000098         9352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDBG010000098 DACDBG010000000
VERSION     DACDBG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.098"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IVZ90_002422"
     CDS             complement(93..761)
                     /locus_tag="IVZ90_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8263425.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IVZ90_002423"
     CDS             895..1182
                     /locus_tag="IVZ90_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263426.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IVZ90_002424"
     CDS             1598..3136
                     /locus_tag="IVZ90_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8263427.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IVZ90_002425"
     CDS             3133..4098
                     /locus_tag="IVZ90_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263428.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IVZ90_002426"
     CDS             4085..5407
                     /locus_tag="IVZ90_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8263429.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IVZ90_002427"
     CDS             5648..6391
                     /locus_tag="IVZ90_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8263430.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IVZ90_002428"
     CDS             6406..7398
                     /locus_tag="IVZ90_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8263431.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IVZ90_002429"
     CDS             7410..8147
                     /locus_tag="IVZ90_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263432.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IVZ90_002430"
     CDS             8137..8859
                     /locus_tag="IVZ90_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8263433.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9352)
                     /locus_tag="IVZ90_002431"
     CDS             complement(8943..>9352)
                     /locus_tag="IVZ90_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8263434.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1605 c         1969 g         2711 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg tt
//
LOCUS       DACDBG010000099         9194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDBG010000099 DACDBG010000000
VERSION     DACDBG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.099"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1277
                     /locus_tag="IVZ90_002432"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IVZ90_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IVZ90_002433"
     CDS             complement(1456..1791)
                     /locus_tag="IVZ90_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8263435.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IVZ90_002434"
     CDS             complement(1775..2047)
                     /locus_tag="IVZ90_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8263436.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IVZ90_002435"
     CDS             complement(2498..3301)
                     /locus_tag="IVZ90_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8263437.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IVZ90_002436"
     CDS             complement(3279..4403)
                     /locus_tag="IVZ90_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8263438.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IVZ90_002437"
     CDS             complement(4419..5234)
                     /locus_tag="IVZ90_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8263439.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IVZ90_002438"
     CDS             complement(5224..6105)
                     /locus_tag="IVZ90_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8263440.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IVZ90_002439"
     CDS             complement(6181..7470)
                     /locus_tag="IVZ90_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8263441.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IVZ90_002440"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IVZ90_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8819..>9194)
                     /locus_tag="IVZ90_002441"
     CDS             complement(8819..>9194)
                     /locus_tag="IVZ90_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8263442.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2829 a         1966 c         1466 g         2933 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca gccagactgt agacaaaagc tgttccaaac
     8881 tcgtaactcg agcttggaac agctttttat ctacttcgtt tcgaattttt gaacaaaaaa
     8941 ataaaggctc cttgcctttc catagccagg tggctaattt ctttaaattc attgcagcaa
     9001 acactagcat cgtatgcgtc gcgacttttt ccaagcctcg atacttggtc caacgcatcc
     9061 catgcttttc ttttgcatcc gcaaatattc gttcaatcgt ttgtttccgt tttttgtaaa
     9121 ttgatttatt cagatctgtt aggcgaagat gttccacttc atccatcaga tctccccaaa
     9181 catgtctcgt gatg
//
LOCUS       DACDBG010000100         9186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDBG010000100 DACDBG010000000
VERSION     DACDBG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.100"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IVZ90_002442"
     CDS             256..531
                     /locus_tag="IVZ90_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263443.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IVZ90_002443"
     CDS             544..2616
                     /locus_tag="IVZ90_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8263444.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IVZ90_002444"
     CDS             2781..4667
                     /locus_tag="IVZ90_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8263445.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IVZ90_002445"
     CDS             4679..5626
                     /locus_tag="IVZ90_002445"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8263446.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IVZ90_002446"
     CDS             5646..6173
                     /locus_tag="IVZ90_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8263447.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IVZ90_002447"
     CDS             complement(6236..6889)
                     /locus_tag="IVZ90_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8263448.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IVZ90_002448"
     CDS             7021..7863
                     /locus_tag="IVZ90_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8263449.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IVZ90_002449"
     CDS             8021..8917
                     /locus_tag="IVZ90_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8263450.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..>9186
                     /locus_tag="IVZ90_002450"
     CDS             8920..>9186
                     /locus_tag="IVZ90_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8263451.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDI"
BASE COUNT         3179 a         1558 c         1908 g         2541 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatatt
//
LOCUS       DACDBG010000101         8997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDBG010000101 DACDBG010000000
VERSION     DACDBG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.101"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            93..677
                     /locus_tag="IVZ90_002451"
     CDS             93..677
                     /locus_tag="IVZ90_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263452.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            939..1661
                     /locus_tag="IVZ90_002452"
     CDS             939..1661
                     /locus_tag="IVZ90_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263453.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1792..2535
                     /locus_tag="IVZ90_002453"
     CDS             1792..2535
                     /locus_tag="IVZ90_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263454.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2541..2906
                     /locus_tag="IVZ90_002454"
     CDS             2541..2906
                     /locus_tag="IVZ90_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263455.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2922..3452
                     /locus_tag="IVZ90_002455"
     CDS             2922..3452
                     /locus_tag="IVZ90_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263456.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3991..4173
                     /locus_tag="IVZ90_002456"
     CDS             3991..4173
                     /locus_tag="IVZ90_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263457.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            4284..4601
                     /locus_tag="IVZ90_002457"
     CDS             4284..4601
                     /locus_tag="IVZ90_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263458.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            4604..4836
                     /locus_tag="IVZ90_002458"
                     /pseudo
     CDS             4604..4836
                     /locus_tag="IVZ90_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4951..5430)
                     /locus_tag="IVZ90_002459"
     CDS             complement(4951..5430)
                     /locus_tag="IVZ90_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263459.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            5550..6887
                     /locus_tag="IVZ90_002460"
     CDS             5550..6887
                     /locus_tag="IVZ90_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8263460.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6910..7437
                     /locus_tag="IVZ90_002461"
     CDS             6910..7437
                     /locus_tag="IVZ90_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263461.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            7424..7987
                     /locus_tag="IVZ90_002462"
     CDS             7424..7987
                     /locus_tag="IVZ90_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263462.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            8409..>8997
                     /locus_tag="IVZ90_002463"
     CDS             8409..>8997
                     /locus_tag="IVZ90_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263463.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADI"
BASE COUNT         3404 a         1128 c         1801 g         2664 t
ORIGIN      
        1 agtttaccat aaaaaaaaat taaaaagcga gacatattta tgttgttgtg ttatcataat
       61 aacataaata tgtagagtga gatgatgaaa aaatggattt gaaacaatcg ttttacatct
      121 ggttatatcc tgataatcct atgtcaataa tggctgatat ttttgtagtt agcatcttat
      181 tgtatttatt ttatctaatt ttctttcaca atttaatcgg gtacatgatt cttgattgga
      241 aaggaatgtt cgatatagaa agaaaaccat ttgctaacaa tcaaacctat accgtcttcg
      301 aaagtggaga ttgggaagaa aatgaagttg atgtgatcca tgaaattgga ttagctttat
      361 caggaataca agatgctgat gttttggtat caatgacaac ggatactgga tacaacgctt
      421 atgaggtatt ttatcttatg tattttatat ggcgtgatgg tcaagaaatt atcaatggtc
      481 gtgctgaaaa aacaatcaat tctaatactg aaatttcttt ttgtatgggt gaaggggaaa
      541 tgattgtata taaagatggg tcagcaaaca ttaaaacagc ctttggtgat attgaaacaa
      601 gtattaagga acttagggaa tatgtgcaga acccatataa ttttcaatat ttagcaggaa
      661 gaataaattt agtttaggag taaaaatatg aaaaaaatta taaatcaacc aacaaatcac
      721 ttgataattg aaggtgttat tttcgtaaca atgtatttta ctgtaggaaa tggcatctca
      781 aatacaatca ttcaaaaaaa tccacaatac ctattattta gtctttatgc ttggattgta
      841 caaatggttt gggtttccat attgatgctg ttgttttatt ggatagtacg ggttattctt
      901 ttattgttaa acaaaagaaa aaaggatagg tgaataaaat gaaatcaact aaattgaagg
      961 ttgtattgtt atcgacagca gcattaataa cccttacagc atgtggtaca actaatagtg
     1021 ctaataataa caaagacaca aagtctagta gtacgatggt tactaaaaac agcactacat
     1081 cggcatcaac aacaatgtct tcaatgactg aaaataatgc tgaaaagtat caaaaatacg
     1141 aagacattaa taaaggtcaa aagccggatg aagtttataa caactttatt aatacagtaa
     1201 aaaatgatga aaattatggg aatgatccat tgcaaaataa aaatctgact atcgttcttt
     1261 acaaacctga ttgtccagat tgtcaaaaag cagaatcaaa aatcatgcaa gaaataaaag
     1321 aaggatttag ttatgctcca acattaatgc atgatattac cataaataaa ccagcttacg
     1381 agtattacgc agttgctgta gatattactg atggtgtacc tgagtgggta caaaacattc
     1441 ctggtctggc tgatgagaac aatcaatatc atacgccaac agttgcagta gtaacaccaa
     1501 agaaagttaa cggcacacta tactgggatg taatgggaat gacaacaaat acaactgatg
     1561 aaatgattga aagtacaact aaatattata tggatttggg agcatataaa ttatatcaac
     1621 aatcagagcc tgttatggac gatcaatcca aagttaaata gatctttagg gcgtaagccc
     1681 tattttttta aaattaagtg ataacaaata aatgatttct gaaacaaacg ttcttactca
     1741 actactcgaa acacacaagc taattaacta gattataaag gagaatgcgt aatgaaagaa
     1801 atcagtgcaa atgaagctca tattccgcaa aagaatcagg ttggtatata taaggtgctt
     1861 catggtgaag gaatttataa aatacagtta cttaccggtt acaataatgg tgcaaatggg
     1921 aagtggttaa aggcaacaag tattcgtgaa cttagaagtg aagtagaaaa aattaggctt
     1981 ggtctcacaa gtgaagaaat agctaaactg aagggaaata attcttttgt aattgaaaaa
     2041 gaaaaggaaa aacatctact ttatcgtgag ctgaaaattg aagaatacta tgaaattgat
     2101 ttacctgaaa aattaagaat tattgatcaa cgttcagaag aaaaatggaa aactgaaata
     2161 tatccaagct taaatgacta caagacatca ggaaagccca tgtataacta cacaggcttt
     2221 ttaacctaca cacaatacaa atatgccttt cgcaaattaa aaggcattaa acaagctgaa
     2281 attgccctgt atgagatgaa aaaagtggat agattgaaat tgattcaaga tttagtgcaa
     2341 aatacatgga atcaagcgat tgtcgagcta gaaaaagaaa atgggaaaat tttaactaat
     2401 aaatatctga tcgtaaatga aattgaagaa ttaattggtt tattttcatg tgaaaaaggt
     2461 gaagtcttct tgaagtcatc accaacctat tcaattttgg acagtccgta tcagtttata
     2521 caggcaagga gatgagttag atgtctagct tgaaagatga ctatatccaa gcaaaacaag
     2581 agtatgaaaa aatacagatg ttgaaatctg agaaagtcga attattttta acacaaaaat
     2641 tagcagagga tcatattgct aacttagatg ctaaaacttt agaggaatat gatcggcagt
     2701 ttaatcagat acatcacaat atcattttgc ttgttgagca ggcaaagcga aggtttgaaa
     2761 aagcggaata tgatctatta gatcaaggaa ttgtatgctt ggataaggga ttgcgaagat
     2821 attatggtgc agagttaaaa aagcaaaatg tagatagaaa tgcactaaaa catgaaatct
     2881 tagaattgat aatgcaacga atttgaaggg atagaaagca aatgtcacaa aacattagtg
     2941 ctgtaaaaca attaatagaa gaagatttag agaccacagt tcagagatta gcggaacgaa
     3001 tccattttgc aaattatcaa accatctcta actggtcaag caggaacaga aatccggaag
     3061 aaattccaat gaaatttttt tatcgaatcg gaaaagaata tggtgttgat ccgataaaaa
     3121 ttttcgagaa gaccatgatt tattactatc atggaacacc taacgaagtg ttagaacatc
     3181 ttttggaaga ggcaaaagaa aaatcatcaa tattagagct atcgcaaaat gacaaagtta
     3241 ttcaagtgaa atgcgttgat ccaacaggaa agattattgt cggttttgat attgaaagaa
     3301 aatccaaaat agattttatt acacaattaa aaaagataaa taatttagat attctaacgc
     3361 ttagaccaga tcatgaacag ttgtttatga gttttaaaaa acaattgaaa gaaatacaaa
     3421 aaagatataa caaggtgttt cctaatgcgt gaaaagctca aagaaatcag agttttttgg
     3481 ataattaaca aagaatgtat catcttatat actcttggat tccttagcat gatcttgttt
     3541 tggtggttat atttaaaaca gtaattggag agggtcaaga ggatttttat caagataagg
     3601 atgtggaaac tgcatgtgga ataatagatt aaaaacagga ttgttattaa tagttatttc
     3661 ctgcgctatg atgattggta tgaggataca aagggaacaa tcctattttg aagtttctgc
     3721 taacaacgta atagaaaaat gctattacgg tcaacattat tggagtgagg aagtcagaga
     3781 aaatatagat cgtgaatatg ttcaaagaat tgtttaccga ctggtcaaat ctcattaggt
     3841 gaaaaagact atctgataaa agtaggaaaa gagttaatta taaaagtacc ggaacactta
     3901 tttaaagaaa attttgagat acgtgaaacc ttggtatgga aagaagaaaa atagtttgga
     3961 agacatagaa tggtataagg ggaaattgaa atggcagtaa ataaaatatt gcaggtacta
     4021 atcatagacg aatttggcgg aggagaatgg tttgatgtgc agtccataga ctttgaagaa
     4081 gaagtcgtag ttatagaaaa tatttacggc gaagtatatt catattcgct tgaaggtgag
     4141 aatattgagg atttacggtt gattggtgag tagaaaaaaa cagaaatatc aaaatggaga
     4201 taaaaattaa tagcatgtaa agatagtcat gcttgggtta aaaatgttaa aattcaaaaa
     4261 aatgagtcag aagggataaa agtatgaaaa gagaggaata caaacaaaga ttaaacgaac
     4321 tgcttgaaga ggacgaaaca ttaactcatg gttcgcctga tgaaattcta tacatgattg
     4381 ataatatggt gatctttggt ggatatgaac ttggtaatag gtcagttgat cataatattt
     4441 tagaatttga tgatgtatca tgggaagaaa ttcttgattg gggaattttg gcggtacctg
     4501 aaactaaaac ctatattagt gacactatgg ttcctttttt tgaggaattg gattataaaa
     4561 gacttcccaa aaatgaaaat catatactgg gaggaaacta acaatgttaa gtgacttatt
     4621 tattttactg atctatctag gtgtaagtga aattattgcg tattttttgt ttgtcattgc
     4681 taattatggt atttccagtg caatcgttca aatgtttaga ttaccagtaa gtgaactaaa
     4741 aaaatgatga gattatcagc atatctttta atgtttttga atgtggtcac gttaatcttt
     4801 gtagtaatcg gtgtgacttt cgatttaatg gaatagtggg aaatctataa tggaaaaaag
     4861 agacacgaat gaacatattt tttgaatcaa tattgttgat ggtttttgcg atcagcgctt
     4921 accagaaatc taaaaaagag taatgattgt ttaatcatca ctcttttttt gtagggaaat
     4981 tgaaatatgt ggagcttgta ttggtttagt taggattgct tggctataag gagctagttc
     5041 tttatcaaaa ccatcaaagt aggttttcag tagcgattta actgatcgct catcttgttt
     5101 gatttggata atttcagcat ccaaatcggt tgtgagatca ggagcttttg atgcgattac
     5161 ctgatttttt ttatcttcca actctcttag gctgtttaac aagttgtaaa aagaatcatt
     5221 ttgattgttc attccaacgt gagttgcaat tttaacagaa gtttcgtccg tattgtcatc
     5281 aaaagaactc attggaatat caagtagtaa cgaggatgtt tcttttactg actttttctt
     5341 tacagcattt tttgtttttc catctttttt gctctttact cgagatgttt ttggagctag
     5401 tttaggtaaa tcacttgttt tatcagtcat ttattagctt ttcctttcta ttaatttatg
     5461 ttattatatt atcataataa cataaaaaag tacacttttt gggggcgtag ttattgaaaa
     5521 aatctaagag aaagattgcc acattgctta tgttaatggt gcaatttaca gggattgcaa
     5581 tgagtgtaat cacaataaat ccgcaagtga tacatgctga tgggatagct acttatgatg
     5641 cacaatttaa ctgtactact gaaggtagtg ggtcaggaac agatagcgat ggaaacgata
     5701 ctggaagtgg cgctgtaaca ggtgatgtag ccaaaaatat tgaagatatt tacaaataca
     5761 tgaatggaga atatggctta tcaggtgcta gtattgcagg tattttaggt aactggaaac
     5821 aagaatcagg tattgatcct cgagcagtag aagggtatta cggagatgga cagagagaag
     5881 ttgacttcat caaacaattt actgatggtc tgatgggtat tggctatggt caatggacag
     5941 ctgggcgaca tacagcatta gttgattggt ctaagaaaaa taatggcggt tcttggtgga
     6001 ctacagaagc ccaattggac tttatgttca aaggtgatgg gggattcgta ggaatattaa
     6061 aagattatgc tttaaatgct tctgatgatg tcgtagaaaa tgcagtaact tttcataaaa
     6121 actgggaaat ttcagctgat tccgaatcag ctgtaatagc tgtacgtggt gaaaatgcaa
     6181 aagaaatctg gaagtacatg aaaagcaagg gtatggacgg tgcaaaagat gaaagtaaga
     6241 tcaataaaat atccggaggt tcaagtaccg gagatggatc gaataattca tcaagttcag
     6301 catcaggaac agtagaagat gcctgtgcaa ctacggatga cgctaattct ggttctggat
     6361 caattgggaa agaaggaaaa attaacggta aaaaaggtca agtaatagcg aatatgactt
     6421 atgaagaagc tttgaaaaaa tatgggaaag cgatcacgct accaaaattc aaagaaccag
     6481 attggtcaac ttcacctttt aacggaatat atcaaggtca gtgtacagag ttgacgtggg
     6541 cttatatgag ccaattatac gatgggaatc aaccgactac tggaaatggt ggttatgtat
     6601 gggaaagcta caaatcggca ggagcaaaaa taacgaacaa accaatcgta ggttatgggt
     6661 ttagtgcttc ggatggatgg ttatgggctg ttggagatgc agggcatact ggtgtagtta
     6721 tagcggtgtt tgaagatgga tcatttctat gtgctaactt taatgttcca ccaacccaag
     6781 ctccaacaag gggagtttcg gtaacgttga ttgatggagt ggacggatca gataatgtgc
     6841 atttctttag cggtgtagga aaagctaaat ttgaaatcga aaaataaatt ggaatgaagg
     6901 acgatataga tgaatagtaa agtgaaagat acaattatca tttctttgct ttctggtatg
     6961 ttattagcac tgcttgttgg aatcctattc ttcaataagc agaatcagga catagaagct
     7021 gaaaaacaca gtgtgcatat tgaagataag ctgacattct ttaaagaggt taaaccaaat
     7081 gaggtggatg gattacggaa agatttagaa aatgatattc aagaaatgtt gaatggatca
     7141 gttcaaaata agtttgcaga tcaatcaggt gaagaatttt tgagaaaact atttattgta
     7201 acaggtaaac caccaattac tgataagagt agtgagaaag aaaaaatcga gtactataag
     7261 ccttttactt ttaagctaac aaactttgga attaagacat ctagtagtgg gttgtatgaa
     7321 gcattgaccg attttgaagt tcaatacaat ggaaaaaaag tgtatgatac cacattaacc
     7381 atcacactaa ataatgataa gaaagtgact gggggtgctt tttatgcaaa atcatgaaat
     7441 cactactgct aaaaaggcga tcggaatact ttccataatt gtgatccttt gttgtgtggt
     7501 actatttcaa aagaaaaatg cttatgataa taatgtcaaa gaattggaag accttaaaag
     7561 taaacaaatc gaagtagtca agaataacca gaaagtgaaa gctatgcagg aagctcaata
     7621 caaagaattg ggaaatgctg aaattaagga acaaagtagg aaattcttta atttatttta
     7681 caactggaat tcatgggaaa aatatacgaa gaatatgaag gaaatacaaa aagaatttcc
     7741 tcagatagta aagtctagtc ctgttgatat ttctggcacg atggtaggca ctggtaatag
     7801 tccaatatct agctatactg ctgattacta tacaacaaat caaaaaggta aaatggttga
     7861 attgattaca cagacaagaa atactccaac aacagcaaca acaacaattt ggcgtggagt
     7921 aacaagcata ggagaaaatc aattgtttta tttggaggat ttagatagct atgtaagagc
     7981 agagtaaaat aaaaaaagag ctaacggtac caacgttagc tctttgtgct gacgatgata
     8041 acaaaaccta ccgaaaaagg attgtttcat cacaaacaca aacagaaaag aattgataaa
     8101 aaacccgaac ctcagaattt ttattaatca attcatttag attataataa attgagctga
     8161 acattacaat acgtatatgt tgtaaaacgt aaaaaaagtc aaaagtaaat actttcttaa
     8221 aaagtcaaat aaaactatta aagacaatat atatggttta tttgacataa gaaaatggtc
     8281 tagatgataa ttgtaatata gaattgtaga cttaaataaa gcataaaata ccttttatga
     8341 taaggtatag ctcttaattt taagcagttt tagatataag attgaaagta aagtggggga
     8401 gaataataat ggcagatcat aaattagaaa agttattaga agaaacgtgg aatccaaaag
     8461 aatttagtga attttttatg gagaattttg aaactgactt agcagtgatt gttaaagatg
     8521 ctcttagaga gcaagggtat cctgaaactg caaactatat aaacattaat tttactttgt
     8581 atactgaaaa taaaggaact tgggattttt gggctacttt agcaaataag gagttaagtg
     8641 ataaatcaga cacaggaatt cgtaactttt ttgaatcaaa cagagatgat tatatgtatg
     8701 ctaacaatca aaataaattg aactttagag tagagtttga tgaaacacct gaagaattta
     8761 tagaaagaca accgcctaaa gaaaatgtag caaaggtatt ggaagatcgt tggaattcag
     8821 atgaaatagt ttcaacaatt agcgaactag gcggacaata tgaaccgcta gtagaagcgg
     8881 ttagagaaga gctaagactg aataagtttc ctgatgttca aaatattgat gtttctcaaa
     8941 ttgaaataaa cgtcaagatc acgaataaac ttgattatgg tagttgggca gatatag
//
LOCUS       DACDBG010000102         8490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDBG010000102 DACDBG010000000
VERSION     DACDBG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.102"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IVZ90_002464"
     CDS             complement(<1..173)
                     /locus_tag="IVZ90_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263464.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVZ90_002465"
     CDS             complement(593..1714)
                     /locus_tag="IVZ90_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8263465.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVZ90_002466"
     CDS             2042..3451
                     /locus_tag="IVZ90_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8263466.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVZ90_002467"
     CDS             3448..4176
                     /locus_tag="IVZ90_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263467.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVZ90_002468"
     CDS             complement(4304..5215)
                     /locus_tag="IVZ90_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263468.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVZ90_002469"
     CDS             complement(5232..6239)
                     /locus_tag="IVZ90_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8263469.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVZ90_002470"
     CDS             complement(6236..8365)
                     /locus_tag="IVZ90_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8263470.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVZ90_002471"
     CDS             complement(8370..8477)
                     /locus_tag="IVZ90_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263471.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DACDBG010000103         8440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDBG010000103 DACDBG010000000
VERSION     DACDBG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.103"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IVZ90_002472"
     CDS             94..1248
                     /locus_tag="IVZ90_002472"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8263472.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IVZ90_002473"
     CDS             complement(1270..2022)
                     /locus_tag="IVZ90_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8263473.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IVZ90_002474"
     CDS             complement(2386..5052)
                     /locus_tag="IVZ90_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8263474.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IVZ90_002475"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IVZ90_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8263475.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IVZ90_002476"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IVZ90_002476"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8263476.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2488 a         1736 c         1496 g         2720 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca tgaagaaggt
//
LOCUS       DACDBG010000104         8280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDBG010000104 DACDBG010000000
VERSION     DACDBG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.104"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..1369
                     /locus_tag="IVZ90_002477"
     CDS             join(207..483,483..1369)
                     /locus_tag="IVZ90_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8263477.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1649..2629
                     /locus_tag="IVZ90_002478"
     CDS             1649..2629
                     /locus_tag="IVZ90_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8263478.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            2751..4169
                     /locus_tag="IVZ90_002479"
     CDS             2751..4169
                     /locus_tag="IVZ90_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8263479.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4240..5709
                     /locus_tag="IVZ90_002480"
     CDS             4240..5709
                     /locus_tag="IVZ90_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8263480.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            5719..6186
                     /locus_tag="IVZ90_002481"
     CDS             5719..6186
                     /locus_tag="IVZ90_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8263481.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6222..7094
                     /locus_tag="IVZ90_002482"
     CDS             6222..7094
                     /locus_tag="IVZ90_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8263482.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7175..>8280
                     /locus_tag="IVZ90_002483"
     CDS             7175..>8280
                     /locus_tag="IVZ90_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8263483.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEES"
BASE COUNT         2835 a         1246 c         1692 g         2507 t
ORIGIN      
        1 gaagcgttag aatcattctc accagaccaa atttcaacca ttaaacagat tctagaaaaa
       61 gtatcttata caatcaatga atatcaagta agtctgacaa gaaaggaaga ttgacatgaa
      121 aaaaatgatt tttgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtaaaata
      181 aatgaaaaca cactggagga atttttatgt caagacgtca acgtagaacc tattcaaaag
      241 aattcaaaca acaagtcgtc gatctttatc tcgcaggaaa gccccgagct gaaatcattc
      301 gagattatga gctgactacc tcggcctttg ataagtgggt caagcaagcg caaactactg
      361 gctcgtttca ggaaaaagat aacttgatcc ctgaacaagt agaactaatt gcattacgaa
      421 agaaaaataa acagcttgaa atggagaatg acattttaaa gcaagcggcg ctgatattgg
      481 gacgaaaaga caagtaattg ataccaataa gcacaaatac tcaatatcag cgatgtgtaa
      541 agtgttaaac atctcttgcc aaacctatta ttatcaagcc aaaccagttg aagatgaatc
      601 agaactggaa gaactcgttc aagaagaatt tattcgcaat cgaaaagctt atggtaccag
      661 gaagctgaag cagtgcttgg ccaatctagg tcttcaagtg agtcaacgca gaatcggccg
      721 tatgatgaaa cagcgtggct tgcgttcaac atacacattg gctcatttca aattccattc
      781 ttctacttgt aacgaagcca agaccgccaa tattctagac ggaaccttcc tacaggaaaa
      841 gtcattagaa gcgattgtaa ccgaccttac ctatgttcgt gttgggaaga aatggcagta
      901 tatctgcctg attcttgatt tgtttaatcg agaaatcatc ggctattcat gcggggagaa
      961 gaaagatgcg acgttagtca aggaagcttt tgcaaagatt ccgtactcgt tgaataatgt
     1021 acaacttttt cacacggatc gtggaaaaga attcgataac cagacgattg acgaaatttt
     1081 atgggccttt aatatcacgc gttccttaag caaaaaaggc tgcccctatg ataacgccgt
     1141 tgcagaatcc acctataaat ctgtcaaagt agaattcgtt catcaatacc gctttgagac
     1201 attggctaat ttgcgattag agctatttga ttacgtccat tggtggaata ccatccggct
     1261 gcacggcacg ttaggctatg agacaccgat cagttttcga cagcagagat tggcgcagcg
     1321 aatccttgat aatgagctcg gatgtgatac cggtggcgag gcaccttaat tatttgaatg
     1381 agcttctgcc ttagagaatc agattcgagg aggctcgttg tcaagggcag tcggaacacc
     1441 agcagcaagc ttacgcaata ccttataatt tttgtcaaaa aaagtgttgc cattccacat
     1501 atatctttaa acagctattg ccagataaaa ataaatgata ttatatatat aaatacttat
     1561 ccgaaaaggg gaatgagaga gtcaaggttc ttggaatgtc gaatagtcga gtaggtagat
     1621 tttctatcta caaccttgtt gtttaaaaat gaaaaaaatt ggaattagag aagttgcgaa
     1681 agaggcaaat gtttctccta cgactgtgtc gagggtattg aatgatcgtg gatatttgag
     1741 tgaggaaaca aaaagaaaag tttttgatgc catgaaaaaa ttagaatact atcctaatga
     1801 actggcgaga gcacttttta aaaacgaaac atataccgta ggtttgattt ttcctacaat
     1861 tactaatcca tttcacgctg agttaattca agatatcgag ttcgagcttt ccagcagggg
     1921 atataaagtg ttgctgtgca atagcttaaa caatccaaca aaagaaaaag agtacttgac
     1981 gatgttgaga aagaatcagg ttgatggaat tatagtagga acgcacaata aagatattgc
     2041 ggattatgat attccagggc tacctattgt agcgattgat agaagtcttg gtgaaaatac
     2101 agttacagta tcttgtgata attacagggg tggggaatta gctactcaac tacttgtaaa
     2161 tcatggctgt cagaatatac tgtgtattcg gggagatagt aagataaagc tacccgcgaa
     2221 tagtaggact ttagcatata aagatgtttt gaataaattc atgctggaac cgttagtcct
     2281 agaagtacca tttgtaaaag aaatcaatga aaaaaatgaa ataattaaga catatctgga
     2341 aactcatcca aatattgacg gggtttttgc aggagatgat ttattggctt ctttggcgat
     2401 taacaactta ggaagttcgg gcaaaagagt tccaaaagag gttaaagtga ttggatttga
     2461 tggagctcaa caaacattga tttataatcc agaattatca actattcaac agcctataga
     2521 tcaaatatct aaagtagcag taaaaaaatt attgaatatg attaagggcg aaaaggagac
     2581 ggatgaactt tcacttccag taaagctcat aaaaaaaaat acagcataag ttaaatcggc
     2641 gaaaaattcc ttcgtcgatt taaaaatatt tttatgaaac gggttgacat atgaaacggg
     2701 ttgacatata ttagttgtga agataaaaac cttttcactg gaggaagaaa atggagaaat
     2761 tattaattaa aagcaataat catcaacttt actaccaacc taaggatgtt tgggtcggag
     2821 atgttatgcc ttacgggaaa gaaggaaaat tctttattta tcatcaacgt gatactagaa
     2881 atccagttcc ttttggagaa ccgtttggct gggctttagc aactacgaaa gattttgttc
     2941 attttgaaga ctttggggaa tctcttttaa gaggggatga caatgagaga gaccagttta
     3001 tctacgctgg ttctgtattt gaggcaaatg gaaaggttca cgcattttat acagggtata
     3061 atcgtgaatt cttagcgcag ggaaagacat ctcaagtgtt gcttcatgct gagagtgatg
     3121 attttgttca ttgggaaaaa tcagaaacag cattggagtt agaaccacaa gaagggtatg
     3181 atattcgaaa ctggagagat ccatttgtcg tttgggatga tgaaaaagaa gaatatttgc
     3241 taattttagg ggcgcgaaag ggtcaggata aacatctaca aaccggacgt ttagtggaat
     3301 ttacatcaaa agatttaact aattgggaat tcaaaggtga tttttgggct ccgggattgt
     3361 ataccatgtt tgaaatgcca gaacttttca agattggtga ctggtggtat cttgtctatt
     3421 cagaatactc agatggaaat aaaatactct atcgaatgag tcaaagtttg gaaggtcctt
     3481 ggattaagcc taaagatgaa gcttttgatg gacgggctta ttatgcaggt agaacagctt
     3541 atgatggaaa acgtcgagtc ctatttggat gggtaccaac aaaagatcaa gaaaatgata
     3601 aaaacaatta tttatgggga ggtactttgg ttcctcatga agtctatcaa aaagaggatt
     3661 tttcacttgg ggtaaaaccc atagacgaag tgtggaatgc attcataaat ttacatgata
     3721 ttgccgatgt agagatagaa acagcagaca caagagaaga agtaacgctt gtcaaaaata
     3781 gtggaacttt gtttaattta gaaacaacaa tttcatttga tgagaatctt agtgactttt
     3841 cactgagatt atataaaaac cttgagacag atgaatctta cgaatatcgc ttcaatttag
     3901 atgaggggcg tcttacattt gataaaaacc cttgttaccc ttggtttcaa tatatggata
     3961 aaggacttat tagacctata tctttaaagc ctaaccaatc ttataaacta aaagtgattg
     4021 ttgatgacaa tataatgaca ctttatattg atggaatcgc tcttaacgct cgtgtacatg
     4081 gttgttttgg tcaagcaatt actattacag caacaaatgg atcaatgact gcaataaata
     4141 ccaagttttc aaatgaaata aagaaataat aaacgtcaat catgtggcag gtctgaaaat
     4201 caaacatgac ctgtcacatt taaaagggag gataaatgaa tggactacaa aaaaattgcc
     4261 gaagatgtat tggaaaacgt tggaggaaaa gagaatatct caagcgctac acactgcatt
     4321 actcgtttac gtttaattct taaagacaaa agcaaagcga atatggaaat gcttgaggaa
     4381 atagaaggtg caaaaggtgt tcttttcaat agtggacagt tacaagtaat tttcggaaca
     4441 ggtataattg aaaaggttta caaagagttc gttgaattga caggagctaa ggaagtatcc
     4501 gtttctgaaa tgaaagacga aggagtcaat aattcaggaa atagacttca acgagctttt
     4561 aaagttttct cggatatctt tattccgatc attccagctt ttattggggc agcaatggtt
     4621 ttaggaattc gttcgttgat taccactcct ggaatgtttg gaatggatgg ctcattcgct
     4681 gatcacaatg tattcctaaa tgatttatcc aaatttctgg gtattattgc aaccacattt
     4741 gattatttac cagttttagt tatgtattct gcaacaaaac gttttggcgg aaaccctatt
     4801 ttaggattac ttgtaggatt tgttatgatt catccaggac tagccaatcg gaatgatttc
     4861 gtcatgggaa ctataaagcc tgattattgg catttatttg gattaagcat tccagcagtt
     4921 gcgtttcaag ggggtgtttt tccagcaatt ttgacttctt ggtttttagc aaaagttgag
     4981 aaaattacaa ataaatattc tcctcaagta cttgcttaca ttttagtccc aactgtgaca
     5041 attgttcttg ccaactttgc actattttta gtgtttggtc cattggggaa ttttatcggt
     5101 aatgttttag gtggaatcat tgatttccta tatatgaaga tgggtgcctt tggtgctttt
     5161 gtttttgcag gcgcgttaca accattagtt gtaacaggaa cacaacatgc tattcaaggt
     5221 attgaggcaa acttagtcgc aacaactgga ttcaattata tccaacctat ttggtcagtt
     5281 tcaattattg cccaaggtgg cggttgtatt ggtatgtatt tactctttaa gaaaaaatct
     5341 aaagatagag atattgcgat ttcaagcttt attccaacac tggtaggtat tactgaaccg
     5401 gctatctttg ctgttaattt gaaatattca atcgttcctt ttgtttgctc gtttattggt
     5461 gctggttttg gtggagcatt tatgaaaatt tttgatgtaa aagccattgg acaaggattg
     5521 actgtattgc caggtctgac tattgctaat ccagttcttc cttatatatt tggtaatctc
     5581 atagcattca ttttaccaat catttttatt ctcgcttatg ataaatataa agtgtttatc
     5641 cctgaagcta aacaccgtaa atttgcagaa aaaactgact caaaaaaatt gccagttcaa
     5701 gtaaagtgag atgagagcat gttaaatttt tttagaaaaa atgaaataag cgttgtagca
     5761 cctgcaaccg gtgtttttga aaaactggag aaattaagtg atccagtctt ttcaaaagga
     5821 atgatgggac aaggatttgc aattgatgca aaaaacgaaa cgattgtatc tccaatcgat
     5881 ggttttgttt caagtatttt tcctacaaaa catgccttag gaataaaaac aaaatcagga
     5941 attgagatac ttgttcatgt aggaatagat acagttgagc taaatggtga gggatttgat
     6001 attaaagttc aagaaggaca aaaagttaag gctggggata ttcttatgac cattaatttt
     6061 gtagtttttg aaaagaatca caagactaaa gatgtcattg ttgtttttcc agactaccaa
     6121 gacgaaattt tcattccgaa acttgaagaa gtaaaacaag gacaagaatt gtttgttctt
     6181 ggctaatgat aagtaattaa taatagaatc gaggaaaatg tatggttttt ggggctattg
     6241 aagctggtgg aacaaaattt gtttgtgcca ttggagataa agatttgaac atatttgaaa
     6301 aagtcagctt tcccacgact acacccaaag aaacaatgaa tttagtttta gattttttta
     6361 atcatcataa agatgattta gaaggtatcg gagtgggatc gttcggtcct attgatattc
     6421 attgtgaatc taaaacgtat ggcttcatta catcaacgcc aaaacttgct tggcaggatt
     6481 ttgattttat tggaagttta aaaaagcaac ttaatattcc aactgcttgg acgactgatg
     6541 taaatgcctc ttgctatggt gaatatgttt ctggaaatgg cagaggcctt tcaagtgttg
     6601 tttactatac cgtaggaacg ggcataggag gtggcgctat ccaaaacgga aacttcattg
     6661 aaggttttag tcatccagaa atgggacata tgcttgtaaa acgacacaat aaagacaact
     6721 tttctgggac atgtccattc cacggagact gtttagaggg cttagctgct ggtccggcaa
     6781 tcgaaagaag atcagggaaa aagggacaag aagtgactga aaatgacgtt acttgggaaa
     6841 tagaagctta ttacattgct cagtgtgcat ataatacaac attgatgtta tcacctgatg
     6901 tcattatatt tggtggcgga gtaatgaaac aaaaacactt aaaaaagaaa gtacaagata
     6961 agtttattga gttgatcaac ggatatgtcg ctattcccga tgttgacaat tatattatga
     7021 caccaaaatt gttagataat tctggtattg taggttgttt agctttagca agagacgtta
     7081 aagaaaaatc ttaatttgga aaaaagatcg tctcgactat ttgtttggtt gatgcgatct
     7141 ttttttcaat tagtaattga agggacgcct aaaagtgaaa aaaaaaatga aagatgaagt
     7201 attagagtat atcaaaaata atttaaagta ttacgatttt tctgctcaag atattgcaat
     7261 gaaattttgt attaaaagaa atgtcgcttc ccactatttg aatcagttat tttcagatgg
     7321 aaaattacta aagaatgatt ctgttagacc agtaatgttc aagtataatc aacaaaagcc
     7381 aaaagactgc ttttctaagt ttattggagc agacatatct ttgaaatcca ctatagataa
     7441 atgcaaagct actgtaatgt atccccctaa tggcctccca ttaataataa aaggaaacag
     7501 tggcgttgga aaaagttttt tagcaagttt gatctatcag tatgctcttg atagaaaagt
     7561 aattcataat gacgcaaaat ttgttgttgt gaattgtgca gattatgcaa ataatccaga
     7621 gctattgtca gcagttttat ttggatataa aaaaggagcc tttactgggg ctgaaaaaga
     7681 tactgcaggg atgctatctt ctgctaatgg aggatacctt tttttagatg aggttcacaa
     7741 tctaagtgca gaaaaccaag agaaattgtt tcttcttatg gattctcaaa agtatcgtaa
     7801 acttggtgaa agtgtgcaat gggagtatgc aaatgttcgc cttatcttag ccacaacaga
     7861 agataaaaat acctctttgt tagcaacttt tagaagacgc atcccagctg aaatcactct
     7921 gccagattac aataatagaa gtacatctga aaaaattcaa ttattatttg aatttcttaa
     7981 agatgaagca attacgattg attcaaaaat attttgttcg attgaacttt ttactgatct
     8041 tttatcgaaa acatttgaag gaaatgttgg agaattgagg aatgaaatca agcttctttg
     8101 tgcagaaggt tatttaggaa ataagagaaa gtcttcaatt tatttagggg acaaactaga
     8161 cagcggtttt tggatagatc cagaaattag tatagattta aaaaaaatgt ttcttaaatc
     8221 actttctgaa attgatttga ttgaactttt tcaaaatcac atttcaattg aagaaagcat
//
LOCUS       DACDBG010000105         8219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDBG010000105 DACDBG010000000
VERSION     DACDBG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.105"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(342..698)
                     /locus_tag="IVZ90_002484"
     CDS             complement(342..698)
                     /locus_tag="IVZ90_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8263484.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(712..1122)
                     /locus_tag="IVZ90_002485"
     CDS             complement(712..1122)
                     /locus_tag="IVZ90_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8263485.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1469..3655)
                     /locus_tag="IVZ90_002486"
     CDS             complement(1469..3655)
                     /locus_tag="IVZ90_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8263486.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3873..4556
                     /locus_tag="IVZ90_002487"
     CDS             3873..4556
                     /locus_tag="IVZ90_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8263487.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4637..5356)
                     /locus_tag="IVZ90_002488"
     CDS             complement(4637..5356)
                     /locus_tag="IVZ90_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8263488.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5668..5874)
                     /locus_tag="IVZ90_002489"
     CDS             complement(5668..5874)
                     /locus_tag="IVZ90_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263489.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6119..6721
                     /locus_tag="IVZ90_002490"
     CDS             6119..6721
                     /locus_tag="IVZ90_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8263490.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6862..8142
                     /locus_tag="IVZ90_002491"
     CDS             6862..8142
                     /locus_tag="IVZ90_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8263491.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8155..>8219
                     /locus_tag="IVZ90_002492"
     CDS             8155..>8219
                     /locus_tag="IVZ90_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8263492.1"
                     /translation="MIPIYICEDDPKQLEVLSTVI"
BASE COUNT         2336 a         1538 c         1347 g         2998 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
       61 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      121 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      181 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      241 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      301 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      361 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      421 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      481 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      541 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      601 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      661 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      721 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      781 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
      841 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
      901 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
      961 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1021 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1081 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1141 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1201 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1261 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1321 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1381 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1441 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1501 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1561 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1621 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1681 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1741 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1801 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     1861 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     1921 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     1981 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2041 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2101 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2161 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2221 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2281 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2341 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2401 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2461 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2521 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2581 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2641 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2701 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2761 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     2821 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     2881 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     2941 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3001 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3061 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3121 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3181 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3241 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3301 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3361 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3421 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3481 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3541 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3601 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3661 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3721 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3781 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     3841 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     3901 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     3961 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4021 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4081 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4141 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4201 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4261 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4321 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4381 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4441 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4501 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4561 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4621 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4681 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4741 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4801 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     4861 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     4921 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     4981 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5041 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5101 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5161 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5221 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5281 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5341 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5401 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5461 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5521 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5581 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5641 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5701 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5761 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     5821 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     5881 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     5941 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6001 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6061 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6121 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6181 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6241 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6301 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6361 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6421 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6481 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6541 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6601 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6661 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6721 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6781 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     6841 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     6901 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     6961 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7021 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7081 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7141 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7201 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7261 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7321 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7381 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7441 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7501 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7561 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7621 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7681 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7741 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7801 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     7861 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     7921 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     7981 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8041 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8101 aaccagcgtt tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt
     8161 cccatttata tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaa
//
LOCUS       DACDBG010000106         8129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDBG010000106 DACDBG010000000
VERSION     DACDBG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.106"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IVZ90_002493"
     CDS             <1..197
                     /locus_tag="IVZ90_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263493.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IVZ90_002494"
     CDS             314..811
                     /locus_tag="IVZ90_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8263494.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IVZ90_002495"
     CDS             898..1290
                     /locus_tag="IVZ90_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263495.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..2779
                     /locus_tag="IVZ90_002496"
                     /pseudo
     CDS             1274..2779
                     /locus_tag="IVZ90_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            3524..5350
                     /gene="ltrA"
                     /locus_tag="IVZ90_002497"
     CDS             3524..5350
                     /gene="ltrA"
                     /locus_tag="IVZ90_002497"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8263496.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5385..6401
                     /locus_tag="IVZ90_002498"
                     /pseudo
     CDS             5385..6401
                     /locus_tag="IVZ90_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6404..>8129
                     /locus_tag="IVZ90_002499"
     CDS             6404..>8129
                     /locus_tag="IVZ90_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8263497.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQ"
BASE COUNT         2592 a         1514 c         1766 g         2257 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag gtgcgaaacg ttcctacttg aaaagagtag gcacagcact
     2821 tcattaaatc aatatttaat gtgtgttgga gaactaaaca cacgaatagt cgaatgacgg
     2881 ggtaacgccc tgaaaggcta ctttaatact ctgactggca gaaacttgag taaacaaggc
     2941 tctgtcagaa gctcggtgaa gtcggcagac agacaccgta acagataatg ctgacgaaag
     3001 attcccagag gtgggcgttg tgtcctatat gccggaagtc tataaaatat ctatggttag
     3061 aatgtagatg agaggtctac tgacgaatcc ttgaatgtac aggtctatat cggggctgga
     3121 tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg tgagtaagac tccgcattta
     3181 tgaaaatcca tataacgtta aaggcgttat caaggtagca ggcttataga ggaaacctaa
     3241 ggatatatgt acagatagtg tgtttggaac gttgaaagct gacaacatgg agattttact
     3301 gtctgtgaag tgttcacatc agaaagttat tgtgagatta gcaatgatat aaggtgtgtt
     3361 aatgtcagtg agagtggtgg cacagtacct atgaaaccgt gataacaagc ggtggaggga
     3421 tagccactag tcatatgaag aatagaatta ctaagaaaaa tggtatgggt tcgagtaaga
     3481 ctaagagatg tacttgctcc tgcaaacaag gagggtacag accatgctaa agaaaaacaa
     3541 gctccgctat aacgagtatt atgatatgca acatatttat gacgagttat acgcacagag
     3601 caagaacggt aataactttt ataagttact tgaaattatt ggttcggaac aaaatatttg
     3661 tttagcttac cgtaatctga aatcaaacag tggcagtaaa acggctggaa cggatggaat
     3721 gacgatagac gatataaaac aactcagcaa tgctgagatt gttgctaccg ttcgggaaag
     3781 ccttagcaac taccgcccca aatcagtcag acgtgttttc attccaaaag ctgggagcga
     3841 taaaatgcgt ccattgggga ttccgtgtat ctgggacagg ctggtacaac aatgtatcct
     3901 gcaagtccta gagccgatat gcgaaccaaa gttccataat cattcctatg gattccgtgc
     3961 gaatcgaagt gcccaccatg cagtcagcag agtaacgaca ctgataaatt tatcaaaata
     4021 tcattactgt gttgacgtag acattaaagg atttttcgat aatgtcaatc atggaaaact
     4081 attaaaacaa atctggacac ttggtattcg ggataaaaga ttgatttgca ttatcagcaa
     4141 aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa aaaggaacgc cacaaggcgg
     4201 tctgttaagt ccactgctct ccctcatagt ccttaatgaa ttagactggt gggtaagcag
     4261 tcagtgggaa acattccagc cgaagaaccg cagtaaaaat gggtggcttc aatacgcaaa
     4321 gaaatatacc aaactgaaaa gcggttttat tgtacgttac gctgatgatt ttaaaatcat
     4381 gtgttcaacc tatggagaag cacaaagatt ttaccacagt accgtagatt ttcttaataa
     4441 aaggctgaaa ttagaaatca gtccagagaa atcaaaggta gtaaatctga agaaaaattc
     4501 atcagatttt ctggggttca aaatcaaagt cattccaaaa ggaagaacga aacacggtta
     4561 tgttgccaaa acagatatga accaaaaagc attaaagaaa gcgaaaacaa atctaaagtt
     4621 aaaggtaaaa gatatagcca gacatacgac tggcttcaat atcagcagat ataatctgac
     4681 agttatcggt atgcagaatt actactgcat agcaactaac gtatataaca acctgacaga
     4741 agtaagttac gctctcttac cgaccatacg aatccgttta aggaacattg caaaatcagt
     4801 tccatttgaa agtacaagtt cagaatttca gagcaggaca aaaggaatca gaccaaaaac
     4861 aaaaatagtg atgattgcgg ataatccgct attgccgatt caaggagtac aacacaaaaa
     4921 tcctatgaat ttctcgcaag atatttgtaa ttttacaaag caagggagaa acaaggtgca
     4981 tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg ttgttggaaa atgaaaaccc
     5041 tgccgatagc gtagagttta acgacaatcg tatttcggca tatatcgcac aacaaggcaa
     5101 ctgctatgta atgaatcgtc gaggaactcc gtccactctg atatgtattc acaaatcaga
     5161 caaaggagat aaccttgaca gatattctaa tctggcattt gtggaaattc ccattgcaaa
     5221 ggcaatccta acagaatcag cagacgaagc aaaatcactg ttaaaaggtt atgttcttaa
     5281 tagtcagcag aagaaaaaac tgaacagaat acggacgaat tacggatacc agacaataac
     5341 tgggaaataa cttataaatc tatttggata tgatggaacg ccgtatgaag cgaaagtttc
     5401 atgtacggtg tgaagcgggg gaaaacttgg agataacatc aaaaagttac ctatcgctat
     5461 aacgtggcag atggaaagaa accttgccag agatttccca tgaaatcaat atcgtcaatc
     5521 tgacttctga tgagaaaaac aaaggcttac ttgaccctta tgtaattatg aaaaatacca
     5581 aagattctga atcactggct attgatattt tgacattcct tacggggatt tcctctcgtg
     5641 atggggaacg cttcccaatc cttagaaaag ccattcgtgc agtaaccaat agtgaagtgc
     5701 gagggttgat gaaagtgatt gaagaattac gggttgagaa tacgccacta agtaccagca
     5761 tagccgacca tatcgaaagt tttacagact atgactttgc ccatctgctt tttagtgatg
     5821 gttatgtaga gcagtctatc agccttgaaa aacaactgaa cattatacag gttgccgact
     5881 tggtacttcc tgacaaggaa acttcctttg aggaatatac cacaatggag ttactatcgg
     5941 tagcaatgct gattgtcatt agtacctttg cgttggactt tatccataca gaccgaagta
     6001 ttttcaagat tgtagactta gacgaagcat ggagcttctt acaggtggca caagggaaaa
     6061 cactatctat gaagctggtt cgtgctggtc gtgctatgaa cgctggggta tatttcgtga
     6121 cccaaaatac agatgacctc ttagatgaaa aactgaaaaa taacctcggc ttaaaatttg
     6181 cctttcgttc cactgacctt aacgagatta aaaaaaccct tgcctttttt ggtgtagacc
     6241 cagaggacga aaacaatcag aagcgattgc gtgatttgga aaacgggcaa tgccttatca
     6301 gtgatttata tggtcgtgtc ggtgtgatac agttccaccc tgtatttgaa gaactgctcc
     6361 atgcctttga taccagacca cctgtgcgaa aagaggtgta aatgtgaaac catcaatatt
     6421 aaacagaata aaatcaaact ggacgctgaa acgtctaggt aaagtggcaa tgacagtggc
     6481 tttcacactt gtgattgcca tttttctttt agccatgctg ggaacggtgg ttcaagctgc
     6541 gggcttggta gatgatacgg tcaatgtggc aaatgaatac agccgatacc cacttgaaaa
     6601 ctatcaactg gatttttatg tggataatag ctggggctgg ctaccgtgga actggtcgga
     6661 cggaattgga aaacaagtca tgtatggact atatgccatt accaatttta tttggacaat
     6721 cagtctttat gtttccaatg cgacgggtta cttagtacag gaagcctatt ccttagactt
     6781 catttccgct acagcagatt ccattggcaa gaatatgcag acccttgcag gggttacgcc
     6841 tagtggacta tcaacagagg gtttctatgt tggattcctt ttactcttga ttttggttct
     6901 tggggtttat gttgcctata caggactgat aaagagagaa accacaaagg caattcatgc
     6961 cctcatgaat tttgtgctgg tttttatcct atcggcttcc tttattgcct acgctcccga
     7021 ctacattaaa aaaatcaatg acttttcatc agacatcagc aacgctagtt tgtcactggg
     7081 tacaaaaatt gtcatgcccc attcagatag tcaaggcaag gacagtgtgg acttgatacg
     7141 tgacagccta ttttccatac aggttcagca accgtggcta ctgcttcaat ataacagttc
     7201 agacattgaa agtattggta ttgaccgtgt ggaaagcctg ctctcaacca gtccagattc
     7261 caacaatggg gaagacagag aaaaaattgt tgcggaagaa attgaagata gaagcaatac
     7321 caatatgacg attacaaaga cgattaaccg tttaggtaca gtcttcttcc tatttgtctt
     7381 caatattggg atttccatat ttgtattcct attaacagga atcatgattt tctcgcaggt
     7441 actttttatc atctatgcta tgtttctgcc tgtgagcttt attttaagca tgattccatc
     7501 atttgatggt atgtcaaaac gagccataac aaagctcttt aataccattt tgacacgagc
     7561 tggaatcaca ttgattatta cgacagcatt tagtatttca accatgctct ataccttatc
     7621 ggctggttat ccgttctttt tgattgcttt tctacagatt gtgacctttg caggaatcta
     7681 ctttaagctg ggcgatttaa tgagtatgtt ttctctacag agtaacgatt ctcaaagtgt
     7741 gggaagtcgt atgatgagaa aacctcgtat gcttatgcac gctcacatgc accgtctaca
     7801 gcggaaactt ggacgctccg tgactgcttt aggggctggg tctgccattg ccagtgctac
     7861 gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt acacaagcag atcactctcg
     7921 accaaacggg caggaaaaat caacactagg aaagcgtatc ggtcaaacca tcggtacagt
     7981 agctgatacc aaagacagaa tggtagatac tgctggtaat ttgaaagaac aggttaagga
     8041 tttgccgacc aatgcaagat atgcagtata tcaaggaaaa tctaaagtga cggataatgt
     8101 ccatgattta accagaagta tttctcaaa
//
LOCUS       DACDBG010000107         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDBG010000107 DACDBG010000000
VERSION     DACDBG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.107"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IVZ90_002500"
     CDS             complement(138..428)
                     /locus_tag="IVZ90_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263498.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVZ90_002501"
     CDS             complement(546..806)
                     /locus_tag="IVZ90_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263499.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVZ90_002502"
     CDS             complement(943..1356)
                     /locus_tag="IVZ90_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8263500.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVZ90_002503"
     CDS             complement(1812..2297)
                     /locus_tag="IVZ90_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263501.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IVZ90_002504"
     CDS             complement(2436..2651)
                     /locus_tag="IVZ90_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8263502.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IVZ90_002505"
     CDS             2875..3675
                     /locus_tag="IVZ90_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8263503.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IVZ90_002506"
     CDS             3694..5562
                     /locus_tag="IVZ90_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8263504.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IVZ90_002507"
     CDS             5555..6046
                     /locus_tag="IVZ90_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8263505.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IVZ90_002508"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IVZ90_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8263506.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IVZ90_002509"
     CDS             6606..>7600
                     /locus_tag="IVZ90_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8263507.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDBG010000108         7454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDBG010000108 DACDBG010000000
VERSION     DACDBG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.108"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IVZ90_002510"
     CDS             <1..177
                     /locus_tag="IVZ90_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263508.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
     gene            190..693
                     /locus_tag="IVZ90_002511"
     CDS             190..693
                     /locus_tag="IVZ90_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8263509.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="IVZ90_002512"
     CDS             753..1142
                     /locus_tag="IVZ90_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263510.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..3576
                     /locus_tag="IVZ90_002513"
     CDS             1129..3576
                     /locus_tag="IVZ90_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263511.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3581..5704
                     /locus_tag="IVZ90_002514"
     CDS             3581..5704
                     /locus_tag="IVZ90_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8263512.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            5701..6705
                     /locus_tag="IVZ90_002515"
     CDS             5701..6705
                     /locus_tag="IVZ90_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8263513.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            6724..>7454
                     /locus_tag="IVZ90_002516"
     CDS             6724..>7454
                     /locus_tag="IVZ90_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263514.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRP"
BASE COUNT         2346 a         1245 c         1706 g         2157 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tggacaagct gtgattgtcg atccaaaagg ggaacggggt
     2641 ggctggaaag aaaccttgcc agaaattgcg gatgaaatca atattctcaa cttaactagt
     2701 aagccaaaaa atcaaggatt acttgatcct tatgtgatta tggagaaaaa gaaggattct
     2761 gaaagtctag caattgatat tctgaccttt ttaacaggaa tttctagtcg agatggggag
     2821 aagtttccag tgttgcgtaa ggccattcgg acggttaccc aaagcaatca acggggatta
     2881 ttacaagtaa ttaatgagct tcgaaaagaa ggctcaccag ttgcggaaaa tatcgcggat
     2941 cacatcgagt cctttaccga ttatgatttt gcccatttgc ttttctcaga tggttcgatt
     3001 acccagtcga ttagtttaga taggcagtta aatatcattc aagtagcgga tttggtttta
     3061 ccagatgcag aaaccacttt tgaagaatat accaccatgg aattgcttag tgtttccatg
     3121 ctaatcgtca tttccacttt tgccttggac tttatccata gtaatcgaga aatcttcaaa
     3181 attgtagact tagatgaggc gtggagtttt cttcaagtag cgcaagggaa aacattatcg
     3241 aataagctcg ttcgtgctgg gcgggctatg aatgccgggg tttactttgt cacgcaaaat
     3301 agtgatgatt tactggatga acgcttgaag aataatatcg ggttgaagtt tgcgtttcgc
     3361 agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggcttgga tcgagaagat
     3421 gaaagcaatc aaaaacgcct acgagatttg gaaaatggcc aatgcttgat gcaggatttg
     3481 tatggtcggg taggcgtggt tcaggtacat ccagtctttg aagaactctt ccatgccttt
     3541 gatacccgtc cacctgttca agagagaatg gagtgagggg atgaaaaaga aactttttcg
     3601 gattgtggga atcgttttta ttagcatact cactctgata gttctattga gtttagttgg
     3661 tacggtagcc gaggcgacag ggctagttga tgatacagtt gaagcaggaa atctatattc
     3721 caaatattca ctgaacaact atcaactaga tttctttgtg gatagctctt gggattggtt
     3781 gccttggaat tggggtgatg gcttagggaa aagcgtgatg tatggtcttt atgccattac
     3841 taatttcatt tggacggtga gtttgtattt gtcgaacgcc acgggttatg tggttcaaga
     3901 ggcttacaaa ttagatttca tttcggatac ggctgaaagt attggaaaga atatccaaac
     3961 attggctggt attactgaaa atggactcca gacctccgga ttttattttg ggtttctatt
     4021 attgatgatt ttagctctgg gggtgtatgt ggcgtatacc ggattattaa aacgagaaac
     4081 cacgaaagca gttcgagccg tgattaattt tgtgatgatt tttctactat cgggttcgtt
     4141 cattgcttat gcgcctacct atatcacaaa aatcaatgac tttagctcag atgttagtga
     4201 agcggccctt acccttggaa ccgagatcgt tgtacccaat tcagaaagcc aaggaaaaga
     4261 tagtgtggat ttgattcggg atagtttgtt ttcgattcaa gtccaacaac cttggttact
     4321 actgcaattt gatgattcca acatagaaga aattggtgaa gaccgggtca acaagattct
     4381 atctgtgagt ccagatgaaa acaaaggcaa agatcgggaa gaagcggtca aggcagagat
     4441 tgaagacaat gacaatgcga atctcagtat taccaagacc atgaaccgtt tagggaccgt
     4501 ggtctttttg gtattgttca atattggcat ttccttcttt gtttttcttc taacagggat
     4561 tatgttgttc tcgcaaattc tcttcattat ctttgcgatg tttctgccaa ttagtttctt
     4621 attgtccatg ttgccgactt atgaaagttt aggaaagaag gcgattattc gtttattcaa
     4681 tacgattatg atgcgagcgg gtgttacttt ggtgattacg actgcattta gcatctccac
     4741 gatgtttttc aatatttcgg caacctatcc atttttcatg gttgcttttc tgcaaattgt
     4801 gacctttgcc ggtatctatt tcaaattggg cgacattatg agtatgttca acctccagag
     4861 taatgatagc cagtcaatgg gaagacgcgt catgcgaaaa ccgcaaatgt taatgaatcg
     4921 aaaattgcgg caactcaatc gaaatgtggg acgagctttg gcttttggtg gtggtgcagc
     4981 agtcggaaat aagttggcaa aggaacaatc aaaatccaaa tttaagccat ccggttcttc
     5041 tctacgaaag aattcacgat taccgaacga tcacgaaata ctatctgact taactaagga
     5101 gaatccagtt tcaaataata agaaacaatc ccgaatgaat ttggctggga gaaaaatcgg
     5161 caaagttttg gatacccaag ctttggtgaa agataagact aaacaagtaa aggatcaggt
     5221 aaggaatacg ccaacgaatt tgaaatacaa tctccataaa ggaatcgaaa agtcgaaaaa
     5281 agcaccagaa gaattcaagc gcggactgtt ccaagaaaaa gcggatcgag tggatttgcg
     5341 agaaaaacaa cgacaacgaa gaaatgcgaa aatggacgaa aaacgcaaga tactgagtga
     5401 agcaacaaat cgtcagggaa aaagaagggc gaatattcca atagataaag cagctcaacc
     5461 tcagaaagat atgatgctga aaaaagaagc accaaagcgg attgtgagag caaatctgaa
     5521 tcccgaaatc aaacgaaaac taactagtca agaaatcatt tcaaaaggga aaagccaatc
     5581 agcaagtaag aaaaattctt tccaacaagt gaaacagagg tccactctgc caaagcagac
     5641 caatcaaaaa gtacaaaaaa gaatgaacca tcgtccaaaa tcaaggtcag gtgagaaaaa
     5701 atgaacttga agaaagtggg tgttatttca ttaacttttc tgaccatctc ttttatgggt
     5761 attttgatgt gtgtgggttt gttgtttggt gaagacagtg agggaaatgg tagttctggc
     5821 acttctcctg gcggaactag tgtctcagaa gaagtattga aacaccgtgc aatggtggaa
     5881 aagtatgcca aagaatcgga tataacagag tatattccaa ttcttttggc gattattcaa
     5941 gtggaatctg gtggcacaat ggaagacgtt atgcaaagtt ctgagtcagc cggattacca
     6001 cctaattcat taagtaccga ggcatccatc aaacaaggct gtctctattt tgcgtctttg
     6061 gttaagcgtt ccaaagaact agggtgtgat caagatagta ttattcaggc ctataactat
     6121 ggtggcgggt atctagatca tgtcgggaaa aacgggaaga agcatagttt tgctttagct
     6181 gaatcttttt caaaggaaaa atcaggtggt caaaaagtcg attaccccaa cccaatcgcg
     6241 attaaggaaa atggtgggtg gcggtataat tatggcaata tgttttactg tttattagtg
     6301 aagcaatacc taactacaac tcagtttgat gacaaaatag tccaagaaat ttttgatgag
     6361 gcatttaagt atgaaggaac ggcctatgtg tttggtggct caagtccaga aacggggttt
     6421 gattgtagtg gcttgaccca atggtgttat gccaaagttg acctgaaact tcctcgggtg
     6481 gctcaggatc aatatgatgc catgactcat attgatctaa aagatgccaa accaggtgat
     6541 ttagttttct ttcattcaac ctatgatgcc ggtacttatg tgacccatgt gggcatttac
     6601 gcaggggaga atcgcatgtt tcatgcagga gatccagtgg ggtggacgaa tctaacggaa
     6661 aactattggc ggcagcatct tatcggagct ggccgataca aataagagaa aagaggaaaa
     6721 agaatgaaga taaaaattga acggaatcaa aaggaaaaga gcccaaaaaa gaaaaaagaa
     6781 cgagttgtat ccgtgggtaa gcatcgaaaa atggtgcttg ccctttggct tttattgtct
     6841 tgtagtcttg cttttggcgt ttataaaaat ttcaccgcca ttgatcaaca tacaactcat
     6901 gaaaaagtgg ttataaaaga gaaggtagtg aatacttctg gcgttgaaag ttttacagag
     6961 gattttgcga aagaatattt ttcatggaaa aataacaaag aagcaattga aaagagaatg
     7021 agtaatctcg aacagtacct tacagaaaac ggtctcgctt taatccaaga tatggttcga
     7081 gcagatattc ccacgagttc cgaagttcaa tctgtaaaaa ttctagatgt tgaaaaacac
     7141 tcggaagaat ttgtcgtttc atttttagtg gagcaacata ttacagaggg aaaaaagacg
     7201 caatcaattt cttctgcata tcgagtgaca atctttgaag atgaaaacag gaatcattta
     7261 gtaactagtt tgccaacaat gattggaaag ccagacaagg caaaatatga agcaaaacaa
     7321 gtagaaaacg attcagaaat tgatgccaaa acaacggaag aaatcaccga ttttttagaa
     7381 acatttttta aactttatcc atcagcttct gaaaaagaat tggagtatta tgttgaaaat
     7441 ggtgcgatgc gacc
//
LOCUS       DACDBG010000109         7326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDBG010000109 DACDBG010000000
VERSION     DACDBG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.109"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1540)
                     /locus_tag="IVZ90_002517"
     CDS             complement(<1..1540)
                     /locus_tag="IVZ90_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8263515.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEK"
     gene            complement(1791..2837)
                     /locus_tag="IVZ90_002518"
     CDS             complement(1791..2837)
                     /locus_tag="IVZ90_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8263516.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(2848..3120)
                     /locus_tag="IVZ90_002519"
     CDS             complement(2848..3120)
                     /locus_tag="IVZ90_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8263517.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(3155..4063)
                     /locus_tag="IVZ90_002520"
     CDS             complement(3155..4063)
                     /locus_tag="IVZ90_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8263518.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(4195..5427)
                     /gene="pepT"
                     /locus_tag="IVZ90_002521"
     CDS             complement(4195..5427)
                     /gene="pepT"
                     /locus_tag="IVZ90_002521"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8263519.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(5442..6563)
                     /locus_tag="IVZ90_002522"
     CDS             complement(5442..6563)
                     /locus_tag="IVZ90_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8263520.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(6560..7261)
                     /locus_tag="IVZ90_002523"
     CDS             complement(6560..7261)
                     /locus_tag="IVZ90_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8263521.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         2050 a         1585 c         1244 g         2447 t
ORIGIN      
        1 ctttttctaa ttgaggtatc gtaccatgac gcaatacagc agcgcgttct aaatcatagt
       61 tattttctgc atcttctaat tcgtgttttg ctttatcgat ttcagcacgt tttgctgaaa
      121 cagaattgac ttcttctttt tctgtttccc actgcatctt catcgcattt gcttcttcac
      181 gaagttcagc taattcttct tgcaaatttt tcaagcgttt tttacttgca tcatctgatt
      241 cttttttcag tgcagcttct tcgatttcca actgcatcaa acgtctagta acttgatcta
      301 attccgttgg catagagttc atttctaccc gaatcgttgc actggcttca tcgatcaaat
      361 cgattgcttt gtccggtaag aaccgatcag taatataacg gtcagataaa gtagctgctg
      421 ctactaatgc gttgtcatgg atgttaacac catggtggat ctcaaaacgt tcttttaatc
      481 cacgcagaat actgatcgta tcttccactg ttggttcttt gactaatacc ttttgaaagc
      541 gacgttccaa cgctttgtct ttttccatat attgacggta ctcatctaaa gttgtggcac
      601 cgatcaaatg taattcaccg cgagcaagca ttggtttcaa cagatttccg gcatccatac
      661 ttccttctgt ttttcctgcc cctacgatat tgtggatttc atcgatgaac aagaggatac
      721 gtccatcact ttttttgact tctttcaaga ctgcttttag tctttcttca aattcaccac
      781 ggaattttgc tccagcgatc aatgcaccca tatctaatga aaaaatcgtt ttatctttta
      841 aattttcagg cacatctttg cgtacgatac gttgagcaag cccttcaacg attgccgttt
      901 tccctacacc gggttctcct atcagaacag gattattttt tgtttttcgt gaaagaatac
      961 ggatgacatc acggatttct tcatcacgac cgatgattgg atccattttt ccgcttttga
     1021 cttgctgtac taaatcaacg ccgtattttt ctaatgcttt gtattgttct tcttggtttt
     1081 gtgaggtcac tctttctcct cctctcatct cttcgatgtt tttacgcaat tctttttcag
     1141 ataatccgtt tttattcaaa taaacagtta acggataatt ttttaatttc atcaacgcta
     1201 aaataacgac ttccgttgaa agaaactcgt cgccgaagct ttcacgtatt tggtctgctt
     1261 cattcaataa tcgaaagaaa ttctggctga ggttttgtcc atattggacg tttccaccag
     1321 aaacaactgg atactcatcc agtagacgat caatttcatg ttcaaaagat tcgacatcca
     1381 atccggcatc cgtatagaaa tttcgaccaa aatgattagg ttgtaaaaag attttccata
     1441 catgggcaat gtcgatatct tgatgttttc gagtgaccgc aatctgttga gcttcagcaa
     1501 tcgcttcttg aagcgtcgta gtcattttct caatattcat acaggtatac cctcctaatt
     1561 aaaggtaaga tcacttattt ctttgatctt tacaaaaact attatatcgc tttggtcaat
     1621 tttggtcaaa atatacacat agttttttga ctttttttct cgacttatat aaatcttaaa
     1681 aaaacaaaat tgattgtatg tatataaaaa aagagccaaa caaacgtaaa attatcttat
     1741 ccataacgtt tttctttgat ttgataaaaa gggtagatct tataaaagac ctacccttga
     1801 ttttctttat tctctttttt tacttctatt atttcttctt tgatcactct tcgccgttca
     1861 gcaatcacat acacaaatcc atagagaaat ggcaagataa aggaactgaa acgatacagt
     1921 aaaagcgaag aaactgcgtc aactgttcca acaactggtc tgaataataa caaatagaca
     1981 aattcaaagg aaccaatacc agcaggcgta ggaataaccc ctgaaagcac tactgcaaag
     2041 ctgataaacg aaatggtcag tagaaaatct attttaggat ggttttcaat cagaatcaaa
     2101 taaggaagta cataccaaaa tacaagtttt aacatattcc atagataaat ccgcaatagc
     2161 gcagtcctgt cttggataat ggtttggacc gtttccctta aagagtagat ctgctgattg
     2221 catgtatcca cccatttccg caaacgctca cttttaaata acttattcga taagctgaca
     2281 aaaaatattt gaacattcaa gctgacggac atcgccaata aaaaggcaac aatcacgatc
     2341 gtcaaaagga taccagcgat gataaaccag atcattgatg gaccattaga ataaaaaaga
     2401 gagaattgga tgaccaagcc agctaatgca taagtgatca ctgccagttt gtacataatc
     2461 atatggagtg ctgtcacccc tgctccctgg gatagcttca ttcctttttt gttataaaaa
     2521 taaatctccg agatcagcgt tcctgtacca aacgaaacga ttcggtaaaa cgcaatatag
     2581 ctagaggtca agaacccatc tttcgttgta aaattcggcg cgaaaggttc agcaatttct
     2641 ttgattgagc gcccttcagc gatttggtaa actgtcccta atataacagc cgctgccaaa
     2701 acaacaacac tggtagacag caattcggca aagatatctg caaatgattg attgattaaa
     2761 taataaataa tggcaaaaat cacaatcagc aaaagcagat tcagaatgat tttagttttc
     2821 gttcctttag acgacatctc ctgctcctta atggaaaaaa taaatgagaa tcaaatcaac
     2881 aagtacacct gcagcgaatc ctatagtaaa cttctttttc cgagttttat gatggaaaac
     2941 ttttcgagag atcaatcctc ccaaaccacc gcctaacagg ctgacaaata tcaaattatt
     3001 ctcaggaaca cgccagccgc tagttctagc ctgatgttta tcatacccca tcagacaaaa
     3061 taagtaaaaa ttcacaatga aaaggtaaaa ccacaaaaga ttgtgtaaca atacatccat
     3121 tactcttctt ccaactttct gttaactact atttctaagc tttttcttct ttctttggtg
     3181 aataaactgt taatcctttt gatacaacag ataattcaac tggaagatca tctgtcgtgt
     3241 ctccatccgt tcgagtgccc acagattttt cctgcgcttg gattttcatg cgtttggctg
     3301 tgaaataagt aatgccgggg atcgtataga atttgcctct catgattcta ggcaaataaa
     3361 aaataaatcg gaaaatatct aaggaaggta cgatcgttac atgaaatttt ccatcatcta
     3421 gggtcgctga gtcgtcaaaa tttgtaaaac cgcccacgga gttagacatc gtgactgtca
     3481 acagttggga ttttcctttc caatgctcct cgtctgtttc taaatccaaa tgatattttt
     3541 tcttcttcag caataactta aaaaacttct tgataaagat ccacgagccg taacgctgtt
     3601 tttcttcttg actgatccaa actgctgtat ctgctaagat tccgattgtc agtgtagaga
     3661 taatgacttc atcattgact tgaccatagt ctattttccg tacattgcct tccaggatca
     3721 cgtcagcagc ttcttctttt ttgaggggaa tctcaagaac acgtgcaagg ttgttgacag
     3781 ttccacctgg aagtaatccg acatggtatt gagataatgt tttttggaac gcttggacga
     3841 tatgatgaat cgtgccgtct ccacctataa ttactaaagt atcagcttct atggcttctg
     3901 ctttcttgac taattccgca tcatcaatcg atgggccagt ttcagctagt tctacatata
     3961 attctggctg cttttctttt acataatctt gaaaccagcg tgctaatttc tcaccctcat
     4021 ctttccctga acttttgtta taaaaaacta gcagcttttc cattctttac ctcctattta
     4081 ctcttcaaaa aagagggcgg gacaaccaat tccattgtcc tgccctttag ccttagacag
     4141 atgacattcc atcattggga cgaacgcctt gtcataagct gttctttttc gattttaact
     4201 ctttgtgttc aattctgcaa tttttacaat cacgtctgtt gctttttcca ttacttgtaa
     4261 agaaacaaat tcgaatcgtc cgtgcatatt ttctcctccg gcaaatagat ttggcgtcgg
     4321 aatacccatg taagagattt ttgaaccatc tgttcctcca cgaactggtt cgatcactgg
     4381 agagatacca agctcgatca ttgcttgttt cgctaattcc acgatgctca tatctttttc
     4441 aatgatttct ttcatgttgt aatattgatc atacatatct actttgattc gttcttgatc
     4501 aaatcgctga ttcaactctt cttggatatg tgtgatttgt gctttgcgtg cttcgaattt
     4561 ttcacgatca tgatcgcgaa tgatataact catagaagct tcttcaggat tcccatccaa
     4621 tgccatcaaa tggaagaaac cttcgtatcc ttccgttttt tctggaactt catcggctgg
     4681 aagctgatta tgaaaatcga ttgctaactg tagagcattg atcattgtat cttttgccgt
     4741 ccctggatgt acatttttcc cttggatcgt aatatttgct tgagcagcac taaacgtttc
     4801 atactgcaac tcacctaaag gaccaccatc gactgtataa gcaaaatcta catcgaattg
     4861 ctcaacatcg aatttatccg ctccgacacc gatttcttca tcaggaccaa aagccacttt
     4921 gatggtaccg tgagggatag aaggattatt gatcaagatc tccattgcag tcatgatttc
     4981 cgcaatccct gatttgtcat ctgctcccag taatgtactg ccatctgttg tgatcaatgt
     5041 gtggccttca taatttttca aatttggaaa atcttttgta ttcaatgtat agcgttcttc
     5101 cgcatctagt acgatcgtag actcgccatc atagttttca atgatttgtg gattgactcc
     5161 tactgcatta aaatctgctg tatccatgtg ggaaataaat ccaatggaac gcacttcatg
     5221 atcaacattg cttggtaatg ttcccataac gaatccgtta tcttcattat agaaaacatc
     5281 agctaagcct aactcttcta attctttttt caattcttgc gcaaaagcaa cttgtgtctg
     5341 agtcgatgga gtcgttttgc ttgttgcatc ggagcgtgtt tctgttttga catatcgtaa
     5401 aaaacgtggc aaaagatttt cgtacatagt attctctcct tttaattaaa ttgaaatggg
     5461 tttgtatttg tttcagaaac gaaaaagtta atatcccaat tttcttcttg tttccaagat
     5521 tcaaatttct cgataaatcg ttgcttgcat aacgattcaa tataatgacc gggatcaatt
     5581 gcaatcaacc cagcgctttg catatcatga gccgtatgat aatagatatc tcccgtgata
     5641 tagacatctg ctctttgggc gattgcttgc ggataaaatt tttcaccgct tccaccgcaa
     5701 atcgcaatgc gcttcacaga ggattttgca ttttttggct ggactattct cagtccatct
     5761 agttggaacg cttcttttac ttgttctaca aatgcctcta tagaaatttc ttgcggcaat
     5821 tcacctactc gtcctaaccc aaacatttcg actggttcat ctatagcgaa aagatcataa
     5881 gcaggttcct cataaggatg agctgaccgc atggcttgaa tcacttgttt ttcgatagtt
     5941 tccggcaaaa taacctcaac ttttgcctct tgaacttgtt ctgtcttccc tactttccca
     6001 atcgctggtt gtgcaccagc ttccggagta aatcgaccgt gtccaatcga ggtaaaactt
     6061 gtaccggtat aatcaccttg tgtaccagca ccagctgcag ctaatacttc acgcattttc
     6121 tgtgcatgat ccacaggtac ataaacggcc agttttttat aatgaatctc atgagtcttt
     6181 actaaataac tctccacttc aataccgaga agttcacaaa accagtcatt cagtccatcc
     6241 caaataatat ccatgttcgt gtgtgctgca tagacggcaa tatcatgttt caacaaatct
     6301 gcatacattt tttcttgtgg ctgatcagta accagccgtt ttacaggacg aaagattgga
     6361 ggatgtttag caatcagcaa atcgattttt ttcttgattg cttcttcgac cacttctgga
     6421 cgaacgtcta atgtcatcat tactcgttgg attggcttat ccaatgttcc gatatgcagt
     6481 ccgactgggt ctccttcttc agctagccac tgaggacaat agctttcaaa cttttggatg
     6541 aaagtgcgtc cgctgattgt catggcttta aaacctcctc aatcatttgg atttcctgct
     6601 cgatttgttt ttgacgtgcc tcttgattag ctgcttgttt gagctgttca cggatagctt
     6661 ttctttgttg aagctctctt gtccattttg atttgaagat ctcacttttc tcttcaagca
     6721 gaaatggccc gaacatcagc tccatttcat tgtaagtaac tggtttttgt tctttttcgg
     6781 ctacaatgat ttcatatatt tttttatttt cttcaagtat tttttctgat tggattttat
     6841 actgattttc ttgcagccaa gatcgtaatg tacgctctcc gatgtttggc tgaaggatca
     6901 aacgttcttg tccgcttaag cgttgattca acttgccagc ttctaggata tctcggatca
     6961 gtgaacctcc catacccgca atcgtgacag ctgtaatacc atcttgtggc tggatagcat
     7021 ctagaccatt tgcaaggcgt acggtgatac ggtcttctaa tccatctttt ttgacttgct
     7081 tacgtgctga ttcataagga ccttgaacga cttcaccagc aactgcatat tcgatttttc
     7141 ctcttaatat caatgcaacg ggtaagtaag cgtgatctga tccaatgtct gctaaacgag
     7201 cggattcagg gacaaagtca cccactgcgg ctaagcgctg agataaatct aaatggttca
     7261 tagcttcact ccttctagat aattatagca ttttccaaat aaaataagga gactaggaat
     7321 aaaaaa
//
LOCUS       DACDBG010000110         6835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDBG010000110 DACDBG010000000
VERSION     DACDBG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.111"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1070
                     /locus_tag="IVZ90_002524"
     CDS             <1..1070
                     /locus_tag="IVZ90_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8263522.1"
                     /translation="LKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGE
                     REKLIKLNETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTE
                     LAKALAENLFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPY
                     TIVLLDEIEKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGV
                     TADGTIPEAVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQR
                     LEHQEILLTISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSK
                     VTITLLDGQLHFKTEELEEIV"
     gene            complement(1221..1892)
                     /locus_tag="IVZ90_002525"
     CDS             complement(1221..1892)
                     /locus_tag="IVZ90_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263523.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(1885..2379)
                     /locus_tag="IVZ90_002526"
     CDS             complement(1885..2379)
                     /locus_tag="IVZ90_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8263524.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(2399..3619)
                     /locus_tag="IVZ90_002527"
     CDS             complement(2399..3619)
                     /locus_tag="IVZ90_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263525.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            3766..5601
                     /gene="lepA"
                     /locus_tag="IVZ90_002528"
     CDS             3766..5601
                     /gene="lepA"
                     /locus_tag="IVZ90_002528"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8263526.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(5695..6822)
                     /locus_tag="IVZ90_002529"
     CDS             complement(5695..6822)
                     /locus_tag="IVZ90_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8263527.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2128 a         1358 c         1342 g         2007 t
ORIGIN      
        1 aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt caagagtccg
       61 ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc gtaacgaaac
      121 ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat aaacgagtga
      181 ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca cgggctggat
      241 tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact ggtgtcggaa
      301 aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat catatggtac
      361 ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc ggagctcctc
      421 caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga cgaaatcctt
      481 atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc aatatcttgt
      541 tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta gatttcaaaa
      601 atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa ggcgtgactg
      661 ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta cgtggaaact
      721 tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca ttgagcctag
      781 acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt ctggaacatc
      841 aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa aatgcttatg
      901 aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta gaaacaccac
      961 tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg attactttac
     1021 ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa tcttccattg
     1081 agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt ttttcaaata
     1141 aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga agctgcccgc
     1201 ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata actttcaaag
     1261 taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc agcacctatc
     1321 gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac aaacagtgtg
     1381 ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca taagaattcc
     1441 cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa gaggaacaac
     1501 caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa tccatagcca
     1561 agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa agagatgata
     1621 ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa ccagtaaaac
     1681 aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg tggcatgaaa
     1741 atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga agtcgaatat
     1801 ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa agcaagtaaa
     1861 gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta aaaatctttt
     1921 tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt tgatcaaacc
     1981 tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta gtgcagcgat
     2041 ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga tcactgggat
     2101 gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg tcagcccatc
     2161 tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa gagcaatcat
     2221 tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag gaagatagat
     2281 cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat ataacaaatc
     2341 acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat cctttttatc
     2401 aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt gacttcagtg
     2461 tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg cgataattca
     2521 tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata ggttcattta
     2581 agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta ttccaaggag
     2641 cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac ggaaaccgtt
     2701 gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc tgctctttga
     2761 tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct ccatgtaccc
     2821 agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga gcttcttgat
     2881 caagatagaa catttctttt acgtgaaata tttcttcagc tacccaatag aacagaacac
     2941 ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct tcattttcga
     3001 aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg ttttgttggg
     3061 caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc tcaaccatac
     3121 cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa agtttatcag
     3181 acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac aaataagaga
     3241 tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct tgcatcaatc
     3301 catggttagc atattctgga taagttccaa ctccagtcac accacccatt ttataacgtg
     3361 caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt ttattttcgt
     3421 ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg ataaaagctc
     3481 gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac acataggaaa
     3541 gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca agattttctc
     3601 taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca gtatagcaaa
     3661 attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt actgcttttc
     3721 gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga tataaagaaa
     3781 atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat cgaccatggg
     3841 aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc tcgcgaaatg
     3901 caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac gattaaatta
     3961 aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt tcacttgatt
     4021 gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc tgcctgtgag
     4081 ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt ggccaatgtt
     4141 tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat cgacctaccc
     4201 gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat cgatgctagt
     4261 gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct cgagcagatc
     4321 gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc tttgatcttt
     4381 gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac ggacggtatg
     4441 gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga tgttacagag
     4501 gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg tgatgtcggt
     4561 tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac agtaacgtta
     4621 gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc gatggtttat
     4681 tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc tttagaaaaa
     4741 ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc attaggtttt
     4801 gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga acgcttggaa
     4861 cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca tgttaataaa
     4921 acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc tgtaacgatt
     4981 caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga ttttgtcggt
     5041 gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga ttatttagat
     5101 gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt tgactttttc
     5161 gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat gtctggttat
     5221 caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt cgatgcatta
     5281 agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt tgaaaaactg
     5341 aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat tggacaaaaa
     5401 atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa atgttatggt
     5461 ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa gaagcggatg
     5521 aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct gaaaatggac
     5581 gaggacgaac cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa ataaaccatg
     5641 tttacatgga tttgccaccg gtttgccacc ggtggttttt ttcggtggca aaaatcaaga
     5701 attctttcta agattttcca tgatatcgac cgtttcattt ctcatcttat ctgttacatg
     5761 cgagtaagta tccatcgtaa ttgaaattct gctgtggccc aatcgttcag agatttcttt
     5821 catttttgcg ccattttcga gaagaagtgt agcatgagta tgtctgagag aatggaagtt
     5881 aaaagagagg gagagtgcat tcgatatcct cctagtattc catttcacta cacttggcgt
     5941 aactagctca ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga gctttccata
     6001 cttcattcga ttttctaatt gttgtttctt atgttttttc agaattgcta gcaaggtttg
     6061 tccaataaaa atcgttcgat tggaactgct tgtctttggc gtaccatata cccatgcgcc
     6121 gtcattcttt accatttgtt tctctacagt aattgttcca tttgaaaaat cgacatcatc
     6181 ccacgtcaga ccacaaactt cgccaacgcg cattcccgta taaaatccaa tattcaaagg
     6241 aatatagaaa ggatgtcctt caggagtaat ttctagcata tgatcaaagt cctcaagaga
     6301 aatgattttt agatcttttt tagtcgttgg tcgtgcttca tattttggta tttttacata
     6361 cagcatagga ttttgcttga ttaatcccca aggataaacc gccatattca gcgcattctt
     6421 aaggacagag tgagtaatag tcattgtttt cttcgagtaa ccctttttaa attcagcatt
     6481 gatgaaattt tgtaaaagag caggggagag atccgtaagt tttttctttc ctaaataacc
     6541 gtttatatga tttttgatgg taaatcggta gttttcatag gtattgtatt ttagatttag
     6601 tttaacgtat tcctccatcc aaaaatcaag gtattgtttt actcgagtat ccgtacctaa
     6661 aaagtattgt cctgtttcgt caatatctga taaaactttt cgtaaagcag cttcggcctc
     6721 tggtcgggtg tctccgccaa ctttctccac tttttttctt gagccatcat cattgatatc
     6781 ttcgccgttt gtataattaa gcgagacaaa taaaaaagcc atttatgtta cactc
//
LOCUS       DACDBG010000111         6508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDBG010000111 DACDBG010000000
VERSION     DACDBG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.112"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IVZ90_002530"
     CDS             complement(207..1169)
                     /locus_tag="IVZ90_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8263528.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVZ90_002531"
     CDS             complement(1171..2610)
                     /locus_tag="IVZ90_002531"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8263529.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVZ90_002532"
     CDS             2795..4741
                     /locus_tag="IVZ90_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8263530.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IVZ90_002533"
     CDS             5005..5877
                     /locus_tag="IVZ90_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8263531.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6173..>6508
                     /locus_tag="IVZ90_002534"
     CDS             6173..>6508
                     /locus_tag="IVZ90_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8263532.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRF"
BASE COUNT         2080 a         1135 c         1155 g         2138 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     6181 tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc
     6241 ggttgctatc gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa
     6301 ctttctcaaa tgtggtggaa cagcacttga atgctttgaa caatacaaag aaaataagcg
     6361 acgttgtggg agaaccgaaa tcatttttcc tgctgaggaa aaagaataca ttgcaaaaag
     6421 atcaactgaa ggttggaccc cagacgtcat tattggccgt gcagagcgaa ccttctcttg
     6481 ttcagtaagt accctctatc gccggttt
//
LOCUS       DACDBG010000112         6437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDBG010000112 DACDBG010000000
VERSION     DACDBG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.113"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..545)
                     /locus_tag="IVZ90_002535"
     CDS             complement(<1..545)
                     /locus_tag="IVZ90_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP8263533.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVV"
     gene            complement(672..911)
                     /locus_tag="IVZ90_002536"
     CDS             complement(672..911)
                     /locus_tag="IVZ90_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263534.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(978..1217)
                     /locus_tag="IVZ90_002537"
     CDS             complement(978..1217)
                     /locus_tag="IVZ90_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263535.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(1331..1672)
                     /locus_tag="IVZ90_002538"
     CDS             complement(1331..1672)
                     /locus_tag="IVZ90_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8263536.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(1659..1988)
                     /locus_tag="IVZ90_002539"
     CDS             complement(1659..1988)
                     /locus_tag="IVZ90_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263537.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(2579..2911)
                     /locus_tag="IVZ90_002540"
     CDS             complement(2579..2911)
                     /locus_tag="IVZ90_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8263538.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(3748..4788)
                     /locus_tag="IVZ90_002541"
     CDS             complement(3748..4788)
                     /locus_tag="IVZ90_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8263539.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(5137..5463)
                     /locus_tag="IVZ90_002542"
     CDS             complement(5137..5463)
                     /locus_tag="IVZ90_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263540.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(5450..6238)
                     /locus_tag="IVZ90_002543"
     CDS             complement(5450..6238)
                     /locus_tag="IVZ90_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8263541.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1923 a         1204 c          868 g         2442 t
ORIGIN      
        1 tgaactacct ggctgtaatc agaaattttt cctcttttgg agacttcata ctgatacttg
       61 taatgagcat cgattagatc aatttcttct ccttcagata aatcagtcat atcggataat
      121 aaaacgcttg gtgtatctac gttataacca gcaagaatat aatttccatt tcccatttca
      181 gtatctagat aataagtaga agctactcct aaactaagcg tgtcgctatt cgctccttga
      241 aaaacaggga gaataaatcc tgtagaagaa aaaacaagtt ttccagcaat ttcacctttg
      301 agatcctttt ctaatgcctg tttttgatta gtaatcagtt gtctcttaga aatatccgtt
      361 aaactggtta aattatcttg ataggtttta gcctgttcct gaatatggat tgtttttgtt
      421 acaccatcta ctttttcagc gattttgtcc ttttgataac cttgataagc aaaataacta
      481 aaaagaagaa aaacaatgat tacgcccacg taaggattaa ataaagtttt attgttttta
      541 ttcatctatc taccttctat atatgaatta atttacctac atctaaccaa gataaaccat
      601 gttaaatttt actattcttt ttattcaata acaatgccac atcataattg atcattttca
      661 tatcataatc gtcaagaaac ttctaataat agttggcaat tggaacctgt ttttacataa
      721 gttcgcctca actgttcaaa gtttttcatt gaacctaaca aacaagcttt ctcttttcct
      781 gttatcttca tatcattgat atattgcatg acagataatc ttttaaggtg tttttgtttc
      841 atcattttta gcaacttttc ttccatttct ttcgacactt tttttctgtt ttcgatttct
      901 tcataattca tgagttttcc tacctttctt gttttttgca aaaataaaaa agcctatgat
      961 cacatacgct ctacacttca gtcttgggtt atctttttct ttcgtttaat tactggccgt
     1021 gctggtttca aatccttgat tccattatgg gttctccaat aatcgaattc ttttcgtaat
     1081 ctttcttttt ccatatctgt tcctttagac agggatttga atacatcttt aggtgaataa
     1141 ccacgagcta acagctgtac ataccttcct tttgtcaaag tatccatatc catcgcctct
     1201 aaaactggtc gcgccatcta attttcctcc taaataaatt ccttactcaa tgcctaaggt
     1261 aatactacct ttttattatt cgtcgtgact ccttctaagc ctttatctgt aaaatttatt
     1321 cctccagttt ttattcttct ttatccgcta caaattcaat gatttcaacg ctgacaatat
     1381 aatcactcaa atatgaccaa gtatcttcaa cagataattc gtctccatta tcatccgtaa
     1441 gcgtcatttt aaaccattca ttaccttcat catcttcagg tagcaagccg aatccaattg
     1501 gattatcagt caatttttga tcgtataaat catcctcgtc taatgctaaa acatctaatc
     1561 ctttacaatt tccgcctctt ctaaacgtca caaatccttt gtaatcccaa cgttggaaag
     1621 aaaccttaat cgtatgtgtg cccaatcgta tattcggatc ataatttttc atttgttgta
     1681 acctcctcat aatcagttag tactataaag gtatctttgt ttttatccaa aaaatctaat
     1741 aaatcttttg tagaagatat ctcttcatca acttcaccat aataaatacc ttcgataatt
     1801 gttgctatat ctgaaaaact aatttttact ggacatttat cgtctaccgc attcaatacc
     1861 gacaaaattg cttctttttc tgcaatctct cgtttgaatt tctttggagc taatcgcatg
     1921 acactcaagt cattttcaag cttttcaatc catgcagttt tactcatttt cgcaaatttt
     1981 tgaatcattc ctctaccacc tttgctatct actaaattag ttttacttca taacgaatat
     2041 ctttatcgtt tttcgttcac tcaaaaatat ataaaataaa tcgactcgtt tatggcgtcc
     2101 gaaacgagaa accctagtag acagcggtaa atgctcgatt gatccaatga tcaacgtcct
     2161 cgcaagtatc tacatgtact gcagcacacg acacaatttc ttgtgcgtcc tccccattac
     2221 gagaagacct actccttatt ccatgagttt gcgtcaaagc ctagatccct tagtagctgc
     2281 tcatgattaa tctaggcgct attctaactt gtctaacttc taaattccct cttgtttact
     2341 ctgtgcatat tatttacaca aattcatttc accactatcc aaacaagggc atttactcga
     2401 ttgaccaatt ttttacgtta ccaatcgtaa aaacctaccc cgtcactccc cgaagggaat
     2461 tgttgataga tcaagtctac tccttatttt tctagtgatt ctaagttcct ttagaagtct
     2521 cgacaatatt ttcaagcaag cgaaaaacga taaaatacaa gttatgagaa tgatcaattc
     2581 agctgattaa acgccccatc ccttttgcgg tacaacgcaa gactccattt tttcttcgac
     2641 caatcagtac accgttttgg tcataaacat gtttgattgt tggatatagc tcttttaaag
     2701 ggataggatc ttttaaaatt atttgttttt gtttcccaat gtaataaatc atttcttctg
     2761 agactttgtc tttctggatt atatatttct tacactgcac aacggtttta ctttgaccag
     2821 ttgatagttt cttcgtgatc ttttctgtag cggtacaccg tttaccgtag tgacaaatat
     2881 cttcgagact caaaattcct gtatatgaca taaaataatc cacccactta ctttaaatat
     2941 ttttaacaag tttttcttgt tatttttcaa aaatagagaa ttgattttcg ttcatgacaa
     3001 acaaaaaaga gggaacgcat tcaaacatgc gttccctcta atttcagcgt tcgtcatacg
     3061 gcaaagagcc ccacggacat ttttataaca ttatgcttag gcatagattc atcgtaatat
     3121 cacgtatttt taaccttaac acagccaaaa aaagtggtat cacttttgaa tatctgttat
     3181 actaaatccg ttacgggtaa taccgtaaca ctgaaaggta aacgcattaa ggggtataga
     3241 cttaatgcca cctgtggacg tcgacgaagg ttaccagcct tccgagtctc acagggcctt
     3301 tctttttttt gtattttcaa ttgtccaaat ttatcgtcca aaagtgcaga acgaaaatca
     3361 attcatcaca agcatagcag acaattaaat ttaaaacaac taaaaatata caaaaaagtg
     3421 taattgaata ttagcagcac tgcctgaaaa gaagtaatat caggcttttc agcttgggga
     3481 aataagtgaa ttacctctct tcatgaatta ttataactca aaaaagcaca tatgcaaaca
     3541 ctagttccct atttttttga gtgaaaaata actaaaatat ctaacgatta tgaatttaaa
     3601 acctacgatt gtgattgttt tcataaactt ttaatctaaa aactccggaa tactaactat
     3661 ttatttgtaa tatttgtaat aaaattaaaa tcataaaaaa gttctaattt aaaattgatc
     3721 atttttatag taattgcttt ttatcaacta ctctaaccaa ttttccgtac taactgttgg
     3781 taattcgtta aatgtttctc tttgttcgtt tgcaagcttt gccgcaatag ctttttcgac
     3841 aaaccaattt tttacataaa cgaaaattag attctgcaaa ttgtttactt tttcagtttt
     3901 ttgtttttga taagctttca gtaaagtcat atataatcct ttgtcagcat caattccata
     3961 agattccagt tcttcataaa cgacaatagt ttgctctaat tcttcagctt ttttctttgc
     4021 attatgaata gtttggacgg tttgttgagc ttcactgtaa cttgaagaaa atgtttttat
     4081 caagtttaat acattttctg gtacaaatgt atttatactg tcctcttgca tgatgtcgac
     4141 gaagttatct agcaaaactt gattttgtaa ctggtcagtt tcagtgtcta ttatgtgtct
     4201 attagtgtct atagaattag aatagaatag attgtctgcg attttcgcaa ttacggttga
     4261 gtccttgata tgactggatt ttgccgtatt tgcgattttc gcaattacgg tagaatcatt
     4321 gatttttcgc ggttttgccg tatttgcgat tttcgcaatt acggtggaat cagtattgtc
     4381 ttcctcttcc atgattttaa agacgtcttg attcgtaact tctggcttgc ctaagtacaa
     4441 taaattaggc atagattcaa tcttaccatc acgtttattt actcttcctc tcttctgttt
     4501 cagaagacct gctttttcta attctttttt gatcttagat actttacctt cgcggcattg
     4561 taacaatttc attaattctt caactgtgaa tatgaagaat atattatcat cagaatctac
     4621 ccagttattt ttgactgaat aatcaaatcg atctttaagt agcatataag caatttttgc
     4681 atcatttgaa agatccatgt atttttcgtt agtaaaaaat accttaggca tttgataata
     4741 tttttcacga taaatttctt ttgatttgta ataattaaaa tcattcatag ccaatcctct
     4801 ttctgtagga tagcgttttg ttcaaaacta tgttacaatg aattaaacaa atatatctat
     4861 ccactatata tttttctact gctgataagt cgccaaactt tgttaaagca gtagtctttt
     4921 tttatctttt tttagtttac cttaaaaaat aaattaaagc aaaccgctct atttcagggt
     4981 ttaagctcat ggtaactata tcacgatatt acaataacat tatatcacga tatcgtaata
     5041 ttacaatatg aagataagcg acttatcagc tttgatacta ctgattttta taaaaaaaat
     5101 atcacgatat tatgatatcg tgatattgaa tgtaaattaa attttatttt gtttctttgc
     5161 tagtttatat ccttcaatca agtattcttc aggtatggat tttaatagcg catcttctat
     5221 aacgtctggt ttacttttgt ctgattcagc aatagtcgtc caatatttga gtgctgacat
     5281 tagaattgga tttatacgaa tgctatctgc tgttcttaca ctacttcctt gatcttttat
     5341 gactttactt atatttttct tggttaattc gatttcttct acttcttttg gtttgtagtt
     5401 ctttttattt ttatttccta gtagtctact tactgcagct tgactgcgat tatcttctgc
     5461 catcaaaatc accaacttct tttaaatatt cttctgtaaa tgctttatag tcatcttcag
     5521 ctgttgaggg tttattaaaa ttgattagat ctgatccagc agcttgcgct tcgtcaacgg
     5581 ttattgttct acgaatataa gttttgaaaa tttttgcacc aatgactcta gaaatttcgg
     5641 ttgctgcagt cgtcatttct ttagaaatgt tataacgctt attatactta gtaaatagga
     5701 ctcctcgtac ttcaagcttg tcgttcgtat attcttttac cgtttcaata gtttcactta
     5761 actttattag tccttttaat gagaaatacg cagcttcaac tggaatgata atttcatcag
     5821 aaacagtaaa tgcgttgact gtaagcattc ctaaacttgg tggacaatca attataatat
     5881 aatcatagtt gtccttgatt ggttgaagta ctcttttaag cctgtgttca cttcctactt
     5941 gagtaaattc cctctctgca gaagaaaggg ctatgtcggc tggcaaaaca tcaattcctt
     6001 tatagtccag aatagcatct gtaatgtcag cctccccttt taatacttca taaattgtag
     6061 gttggtttgt tccatctata tcgaaaccta cattatctgt agcatttccc tgaggatcaa
     6121 tgtccactaa aagtacattt ttaccgtaaa caaatttcaa acatgctgct aaattaacgc
     6181 tagtcgtagt tttagaaact ccaccttttt gattggctac tgacaatatt cttgccatta
     6241 aaatatcccc cattcatctt atattataat ataattatat cataatattg tgatattgca
     6301 atatcgtata tgcattatat tgtaatattg tgtgattatt ttgttttaaa gatatatatc
     6361 ggttcggata taaagattat tcatatgtta ttcgaaagta aaataatcac tttttattac
     6421 tttaaaattt tttatta
//
LOCUS       DACDBG010000113         6396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDBG010000113 DACDBG010000000
VERSION     DACDBG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.114"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..677
                     /locus_tag="IVZ90_002544"
     CDS             <1..677
                     /locus_tag="IVZ90_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8263542.1"
                     /translation="ISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            688..2892
                     /gene="topB"
                     /locus_tag="IVZ90_002545"
     CDS             688..2892
                     /gene="topB"
                     /locus_tag="IVZ90_002545"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8263543.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            3131..3724
                     /locus_tag="IVZ90_002546"
     CDS             3131..3724
                     /locus_tag="IVZ90_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8263544.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            3740..4639
                     /locus_tag="IVZ90_002547"
     CDS             3740..4639
                     /locus_tag="IVZ90_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8263545.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            4642..5127
                     /gene="ssb"
                     /locus_tag="IVZ90_002548"
     CDS             4642..5127
                     /gene="ssb"
                     /locus_tag="IVZ90_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8263546.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5493..5753
                     /locus_tag="IVZ90_002549"
     CDS             5493..5753
                     /locus_tag="IVZ90_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263547.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            6194..>6396
                     /locus_tag="IVZ90_002550"
     CDS             6194..>6396
                     /locus_tag="IVZ90_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8263548.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         2320 a         1130 c         1313 g         1633 t
ORIGIN      
        1 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
       61 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
      121 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
      181 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
      241 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
      301 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
      361 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
      421 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
      481 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
      541 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
      601 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
      661 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
      721 caagcgaaag cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
      781 atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc
      841 gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg
      901 cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt
      961 gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt
     1021 gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc
     1081 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag
     1141 ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc
     1201 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
     1261 ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa
     1321 ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
     1381 atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa
     1441 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg
     1501 atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg
     1561 agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc
     1621 atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt
     1681 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa
     1741 gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
     1801 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
     1861 caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
     1921 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
     1981 ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag
     2041 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
     2101 gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacagcc ttataccgaa
     2161 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
     2221 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
     2281 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
     2341 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
     2401 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
     2461 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
     2521 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
     2581 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
     2641 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
     2701 aaactcacag ccacacaact gaaagagatc attcaaaacg gccgaacaag ccaatcagtc
     2761 agaggactaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
     2821 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
     2881 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
     2941 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
     3001 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
     3061 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
     3121 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
     3181 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
     3241 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
     3301 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
     3361 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
     3421 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
     3481 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
     3541 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
     3601 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
     3661 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
     3721 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
     3781 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
     3841 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
     3901 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
     3961 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
     4021 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
     4081 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
     4141 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
     4201 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
     4261 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
     4321 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
     4381 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
     4441 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
     4501 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
     4561 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
     4621 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
     4681 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
     4741 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
     4801 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
     4861 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
     4921 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
     4981 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
     5041 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
     5101 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
     5161 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
     5221 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
     5281 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
     5341 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
     5401 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
     5461 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
     5521 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
     5581 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
     5641 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
     5701 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
     5761 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
     5821 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
     5881 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
     5941 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
     6001 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
     6061 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
     6121 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
     6181 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
     6241 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
     6301 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
     6361 cgattacatt ttgattgatg agaaaggaaa gaaacg
//
LOCUS       DACDBG010000114         6347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDBG010000114 DACDBG010000000
VERSION     DACDBG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.115"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IVZ90_002551"
     CDS             complement(99..1229)
                     /locus_tag="IVZ90_002551"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8263549.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKE
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVZ90_002552"
     CDS             complement(1878..3080)
                     /locus_tag="IVZ90_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8263550.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVZ90_002553"
     CDS             complement(3140..3343)
                     /locus_tag="IVZ90_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8263551.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVZ90_002554"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVZ90_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVZ90_002555"
     CDS             4880..5692
                     /locus_tag="IVZ90_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263552.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1895 a         1299 c         1001 g         2152 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaatttctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggtgcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatac
//
LOCUS       DACDBG010000115         6305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDBG010000115 DACDBG010000000
VERSION     DACDBG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.116"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IVZ90_002556"
     CDS             334..714
                     /locus_tag="IVZ90_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8263553.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IVZ90_002557"
     CDS             696..1610
                     /locus_tag="IVZ90_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8263554.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IVZ90_002558"
     CDS             1855..2544
                     /locus_tag="IVZ90_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263555.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IVZ90_002559"
     CDS             complement(2665..2751)
                     /locus_tag="IVZ90_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8263556.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IVZ90_002560"
     CDS             3427..4377
                     /locus_tag="IVZ90_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8263557.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IVZ90_002561"
     CDS             4398..4931
                     /locus_tag="IVZ90_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8263558.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5569..6102
                     /locus_tag="IVZ90_002562"
     CDS             5569..6102
                     /locus_tag="IVZ90_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263559.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2319 a         1103 c         1179 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt tttctagttt gttataacaa tgccactcta acaaaaatgt
     5461 aagcattatc cttattaaaa gctccttgac aaggactata ccttttattt tatgattttc
     5521 acaaagaaaa cgttttctat atatttttct gtatcaggag gaaattctat gaaaaaattt
     5581 actttagggg tagctttgtt aactattggg gcagttgttt taccgtcaac tactatagtt
     5641 tatgcttcag aaaattccca agtggtatca ttaaacgaaa acaatgctac tgaaaatgta
     5701 gttattgagt acactgaaga tggtatggtc gtaactaaat atgaggatgg ttatagtgcg
     5761 gaagattttg caaattcttc agatatcatg acaagagctg cagttgcaag atggggcaaa
     5821 aaggaatata caaatattgc tgtaaccact ggagtagctg ctaatgctat taatgctgcc
     5881 ttttgggcag gagggaaagc tagcacttct atgtttggta tcccaggttg ggcaattggt
     5941 gctttgttaa caggtgcaag ttggactaaa aaaggtgctt ctccagggaa tgcagttgca
     6001 aaagaatggg acaaaaatgg taatggctgg attggatttt attaccaaag gggatatgat
     6061 gcagctggaa gagttgtagc tactagatat tcaacgttat aagttaagga gtgatgtttt
     6121 tgatatctaa atcattaaaa tcattaaaaa catttactaa aaagaatccg caaatagcta
     6181 ctattcgtat ttttatattt gctatagctg ttgctataat tgttaatatg ctaagcggag
     6241 ggcaattata caaatctggt ttatttttag tgttaatctt agttgtctta gacataataa
     6301 agtct
//
LOCUS       DACDBG010000116         6234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDBG010000116 DACDBG010000000
VERSION     DACDBG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.117"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IVZ90_002563"
     CDS             1..1452
                     /locus_tag="IVZ90_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8263560.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="IVZ90_002564"
     CDS             1801..2433
                     /locus_tag="IVZ90_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8263561.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="IVZ90_002565"
     CDS             3021..4460
                     /locus_tag="IVZ90_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8263562.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="IVZ90_002566"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="IVZ90_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="IVZ90_002567"
     CDS             5222..5437
                     /locus_tag="IVZ90_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263563.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="IVZ90_002568"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="IVZ90_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2049 a          996 c         1259 g         1930 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gata
//
LOCUS       DACDBG010000117         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDBG010000117 DACDBG010000000
VERSION     DACDBG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.118"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IVZ90_002569"
     CDS             725..811
                     /locus_tag="IVZ90_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8263564.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVZ90_002570"
     CDS             complement(932..1621)
                     /locus_tag="IVZ90_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263565.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVZ90_002571"
     CDS             complement(1628..1906)
                     /locus_tag="IVZ90_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263566.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVZ90_002572"
     CDS             complement(1866..2780)
                     /locus_tag="IVZ90_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8263567.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVZ90_002573"
     CDS             complement(2762..3142)
                     /locus_tag="IVZ90_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8263568.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVZ90_002574"
     CDS             complement(3592..3879)
                     /locus_tag="IVZ90_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8263569.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVZ90_002575"
     CDS             complement(3899..4123)
                     /locus_tag="IVZ90_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8263570.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVZ90_002576"
     CDS             complement(4763..5296)
                     /locus_tag="IVZ90_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8263571.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVZ90_002577"
     CDS             complement(5317..6159)
                     /locus_tag="IVZ90_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8263572.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDBG010000118         6146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDBG010000118 DACDBG010000000
VERSION     DACDBG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.119"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IVZ90_002578"
     CDS             101..2542
                     /locus_tag="IVZ90_002578"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8263573.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IVZ90_002579"
     CDS             complement(2872..3576)
                     /locus_tag="IVZ90_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263574.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="IVZ90_002580"
     CDS             complement(3763..4008)
                     /locus_tag="IVZ90_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263575.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="IVZ90_002581"
     CDS             complement(4025..4876)
                     /locus_tag="IVZ90_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8263576.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="IVZ90_002582"
     CDS             5042..6046
                     /locus_tag="IVZ90_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8263577.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1811 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtg
//
LOCUS       DACDBG010000119         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDBG010000119 DACDBG010000000
VERSION     DACDBG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.120"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IVZ90_002583"
     CDS             <1..395
                     /locus_tag="IVZ90_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263578.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IVZ90_002584"
     CDS             398..1228
                     /locus_tag="IVZ90_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8263579.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IVZ90_002585"
     CDS             1244..2095
                     /locus_tag="IVZ90_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263580.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IVZ90_002586"
     CDS             2092..2451
                     /locus_tag="IVZ90_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8263581.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IVZ90_002587"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IVZ90_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IVZ90_002588"
     CDS             2624..2929
                     /locus_tag="IVZ90_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263582.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IVZ90_002589"
     CDS             2929..3240
                     /locus_tag="IVZ90_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263583.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IVZ90_002590"
     CDS             3407..3823
                     /locus_tag="IVZ90_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8263584.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IVZ90_002591"
     CDS             3823..4491
                     /locus_tag="IVZ90_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8263585.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IVZ90_002592"
     CDS             4488..4865
                     /locus_tag="IVZ90_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263586.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IVZ90_002593"
     CDS             4862..5161
                     /locus_tag="IVZ90_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263587.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IVZ90_002594"
     CDS             5403..5819
                     /locus_tag="IVZ90_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8263588.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDBG010000120         5813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDBG010000120 DACDBG010000000
VERSION     DACDBG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.121"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..353
                     /locus_tag="IVZ90_002595"
     CDS             <1..353
                     /locus_tag="IVZ90_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8263589.1"
                     /translation="TPLFKGLTIRKKYGKGRGKPVIGYRFTWKAEINHADDFSKGKQE
                     DLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEANFKKQQQTEKEEVEKQTLSNE
                     LKQDLLGNLQNLFD"
     gene            370..885
                     /locus_tag="IVZ90_002596"
     CDS             370..885
                     /locus_tag="IVZ90_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8263590.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            1181..1462
                     /locus_tag="IVZ90_002597"
     CDS             1181..1462
                     /locus_tag="IVZ90_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263591.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            1464..1880
                     /locus_tag="IVZ90_002598"
     CDS             1464..1880
                     /locus_tag="IVZ90_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263592.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
     gene            2537..3691
                     /locus_tag="IVZ90_002599"
     CDS             2537..3691
                     /locus_tag="IVZ90_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8263593.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3685..4107
                     /locus_tag="IVZ90_002600"
     CDS             3685..4107
                     /locus_tag="IVZ90_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263594.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            5230..>5813
                     /locus_tag="IVZ90_002601"
     CDS             5230..>5813
                     /locus_tag="IVZ90_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8263595.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELT"
BASE COUNT         2229 a          963 c          930 g         1691 t
ORIGIN      
        1 tcacaccatt atttaaagga ttaacgattc gtaaaaaata tggtaaaggc cgaggcaaac
       61 ctgttattgg ttatcgtttt acttggaaag ctgaaattaa tcatgctgac gatttttcaa
      121 aagggaagca agaagattta cgtacaaaat tatttaatat tgaacacaat ggagaattaa
      181 cgcaagaaga aaaatggcgc gctaaagatc gaatactaga tcttccgtta ggtactcatg
      241 aagctaattt taaaaaacaa caacaaactg aaaaagaaga agtggaaaaa caaacacttt
      301 caaacgaact taaacaagat ttattaggaa atttacaaaa cttattcgat taaagtataa
      361 ggagtattta tggcagataa aacgatcaaa gaccttgctg aagaattagg agttagtaag
      421 acagctatta acaaaaaagt ttctgacgaa aatcgaaaac tttggtttgc gaaaatcgga
      481 aacaagtttg tgattaacga aatcggtcaa aaagctataa aatcaatgtt ttttaccgaa
      541 aacgaaaact caaatcaaca accagttggc gaaaaatcga aaaccgaaaa ccataaaaac
      601 gccaaccaag tttccgaatt agaactcttt tttttcctag aaaaacaatt aaatcaaaaa
      661 gatatacagc tacaagaaaa agacgaacaa ttaaaacaaa tgcaaaaatt actcgaccag
      721 caacaacaac tcaccttaca agccaatcaa caaatcaaaa aacttcacga acaactccag
      781 ctaacctatg cagaagaatc ttcagaaaaa gaaccccttt ctgttactga agaaactcaa
      841 aaaggacaaa acaaggagaa aaaatggtgg caattctggc aataacccaa caatttttat
      901 tcataaaaat tgttgggtta ctttcttttt ttgcttttac aagcatagcg acatgatctc
      961 acgtcgctat tttttaaaga gcataaggaa acgcggtatc actttcttac gaaaggtgct
     1021 aggtaggctg tgcctaccac gtttctaaga aaaaaatttg ctatcacttt ttcgccattg
     1081 tggctagctt atgtatagta tttcggtaaa aaacagctat acaaatacta tatttgtatg
     1141 atatacataa attatacaca cataagaaag gacgttttct atggacattt tagttcgaaa
     1201 tatcaatcca aaaattatta caagagtaga taaacgttgt caggaactta caaccaaaac
     1261 aggagaaaaa tggagtcgaa ataaatattt aaaagtgcta atcgaaagtg attttgaccg
     1321 tcaacttatg aattacaaaa aagagcaatt cgatttatta ctcgaaaaat ttatcgatat
     1381 tcaaacaaaa aatactgaaa cattgaacga ctatgttcat acaaacaacc aactcattga
     1441 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gttacccttt
     1501 atcccagtca cattgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     1561 actcggaagc cattcgcttt ttatgtatga gttatgaacc tgataccaat acaacaacta
     1621 ccagtaacca agccaaacta aatgcgatgg gaaaagaaat ttctatcatg accgaaatta
     1681 tcgcagaatt tgctgatttg tatttaaaag atacaggcgt tcttatcggt acaaactcga
     1741 aagtatatca agaagtaaaa gaaaaaattg aaaaacagct aaatccctcc caacgaaatc
     1801 aaccaaagaa tcgtcaaaaa gaaacgagtc aaacattcac ttctaaaaat ttcgacccaa
     1861 ccaactttct aaaaagataa aaaaataaac caggaaattt ttaatttcct ggtttatttt
     1921 ctagagagcg gtcttccaat aagatttgca aatcgatccc aatttgcgta aaaaaaaagc
     1981 tgctcgtacg agcagctaaa caagaaaaag tcagcttgtt ttaagggtaa aataagtgta
     2041 acaaattttg cgaatgcaac gatctttttt attatacgca aacaatcaaa aaaaaagact
     2101 gctcgaaaga gcagtctcaa gcaaaaaata aacgaacaaa cgcaattact cacgaacaca
     2161 aacaaataca ataaaatacc taaataatga aaataagtga taaaaaacaa gggtatttat
     2221 agtttaacaa gcgctcataa aaataacaag tctttaaaaa atgacagata aaaatgcaca
     2281 aagaatcgga cacagataac cgcgaaaaat caagctttag aaagttgttt ttagctagac
     2341 caatgaaaaa tcacaaagtc cgcccctgta tccgttggta gaggggctac ttggtttagt
     2401 atacattatg ttaactagct cgttaaataa cgattatgtt cccaaaacaa tattttatga
     2461 actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt atatcactta
     2521 attttaacac aaaggcgtga ttattttgaa acccaaaaat aaaatttatc taggtctcct
     2581 cttttccttg atgattggaa tttttagtat aggaattaca aatacctatg cttcagaaaa
     2641 ttctaatttc aatatcaact cccaaccaat tgaagaacaa gcaattaatt ttgcgaaaac
     2701 tgattttgaa cactccataa cttcaattct tttaatggaa aataacactg aaactgatat
     2761 taactatact cttggttcgc catttaaaat gctcgatgga gaaaatacgg caaatagttc
     2821 tattttcttt ccagtatttt cagacgctga taatacgatt aaatatgttt ataccgtcaa
     2881 aaaaaataca aatggagaat attcaggaag tatttcacaa tttttagcaa aagaacttca
     2941 aaatctagta gatagcaata taacagatac aaccttattt tttcaagata atagcattta
     3001 ttattccgat aatggccaaa ttaaactact ttggaaatca cctttaagta cagaaaaaaa
     3061 tggtgtctct tcctttacag aaaaaaatga tcttatatcc gtaaatataa aaaataaaat
     3121 taaaaatttc tctaaaaaac aaaattttcc tgcggttaaa cgtgtaaaaa gtgtctcctc
     3181 cggagataac tttattatga ttaattggcg tatagcagaa acccaaacaa gttttccgtg
     3241 gtgcgctgct tacgtagaag ctgctatctt atgtaataaa acagatgtac gcgctacttc
     3301 agctagagcc attatgcatt atacctatcc taatttaaac aacagtcaaa ttgaatcaaa
     3361 aagtcttaat caagatcaaa taataaaata tgcaaattac tgtggtgttt atcctaaaaa
     3421 agtagcacga atactaaacc tttcagaggt ccaaacacaa ctaagaaaag gaaatgccat
     3481 ttatatggga actgctggtt atggaaatta tagtaacagc cgtcatgcac ttgcattatt
     3541 tgggtgggtc aatcaaggtg gttatcaaac ttactatgtg tggaatcctt ggtataacta
     3601 cccaactgta ataagtgcaa atgcagggcc agttacaatt cctgtacctg gaggtggttt
     3661 tacttgggac aataccatta caaattggta aataaaccca ttgttttagt tatgggtcta
     3721 gtgttagttc ttttcttaac tagttgttca aaaaaacaag aaatacaaaa gactgagctg
     3781 attccttttc taggaacttg gcaaactaaa gatcactcag aacgtctagt aatcactaat
     3841 ttaaatactt caaaaaatat agaacaatat accatcttag taaaagagaa caatcataaa
     3901 attcaaatca ttgataaaaa tttaaatata aatgaacaag ttttcattta caaagcaaca
     3961 aataatttac taacactgcg ctctaataaa aaacaattag attacacttt tagtttggat
     4021 tctccaaaaa agctcgtatt tattttgaca acgaatgatg ggacagaagg gacagctaaa
     4081 ccaattgaat tttttaaagc tgattgatga atagttgttt gatattacaa aacaataatt
     4141 tcaccgaagg tgaaacaagc gaaactttac aaaaagtggc tagcatttgc tagccttttt
     4201 tgtattgcgg agcgtgagtt ttgaggggga ttcaaggggg agtttctccc ctttgacaag
     4261 cggttatttg caccaaatac gaaggtattt ggctagcaaa tatagagctt gccaaaccct
     4321 ttagtgtgtt ttgtagaaag ggaccttata gtcactttca cattacaaac gatacgtcct
     4381 atggacgtat caaaagggtg ggacttatga gcaaattaaa gcaaaatttg gctagcaatc
     4441 cttctaaaaa atacacctat tgatcagagt caaaataaat cagttttcga gtgagcgaat
     4501 ccgaatttgc aaatctgaag ctttgcaaat gagtgtgccg gcttccgtaa aagtctaggt
     4561 aacattagtc gatatataca taaggctcta atttgcgttt taaggcgttt taaacgttct
     4621 ggtatgtaat ttataccaaa gcgactttaa aacgccttaa aacgaatttt ataggtcttg
     4681 taggacgttt cagggttgtt agggcttgcc ctgaccgcct gtaaggcgct ttaaaaacaa
     4741 gataagggat tttatctaga cttgtcttaa aacgccgtag atgagcgctc agagcttctt
     4801 aaaggactcc ttacgaactg tttttaagca aaggagcctt taggcgactg cgcagttcaa
     4861 tgtgagagcg tagcgatcac acctttaaag cgaccgtagg gagcagattc ccagtttagg
     4921 aaatttcgcg aacctgtttg gagaaaaaaa gtaggaaatt tttaggcggg aaattttgcc
     4981 cccctctttt ataatctctt ttataacctc tttttaaacc tctttaccgt cggctgtatc
     5041 cttactctcc caaggcctgt agctttttta gggcatgaaa ttatctgttt ataggcatga
     5101 aattatctgt ttataggcat gaaattatct gtttataggc atgaaattat ctgttttagg
     5161 tattgtttta agacataata taccttaaaa ttatgtcaat aaaattagtt agactggagt
     5221 atcaaataaa tggcaaatga aatcgtaaaa tataaaccag aattaaatac cattccttta
     5281 aggaaattta ctccaataga aatgaacttg tttttttcta ttgtttctag aatgcgtgat
     5341 aaaggtaatc aaaaaatacg cttttatttc gatcagctta aagatttaag tgcttataag
     5401 caaactgctg gagaagtctt tataaaagat ttaacaagaa catacgatca tttaatggat
     5461 ttacgttttg gaaaaaaaag caatggtgga ttaaccatag aacgatttac aatgtttact
     5521 gattttgaaa ttcatggaga agccgataac ccttatgtgg atataaaagt tcacgaaaaa
     5581 gctttgcctt tacttaacaa tttagaaagt tgggtacgtt tctctttaac agaatttcga
     5641 gatttaaata gcagttatgc taaaacaatg tttagattac taaaacaata ccgaacacaa
     5701 ggatgggctg aattttccaa ggaagatttt ttagaactat tagatattcc taaaagctat
     5761 agacaaacaa atataaatca gtttgtactc aaacccataa aagaagaact cac
//
LOCUS       DACDBG010000121         5680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDBG010000121 DACDBG010000000
VERSION     DACDBG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.122"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..1287
                     /locus_tag="IVZ90_002602"
     CDS             133..1287
                     /locus_tag="IVZ90_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8263596.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="IVZ90_002603"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="IVZ90_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP8263597.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="IVZ90_002604"
     CDS             3322..4584
                     /locus_tag="IVZ90_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP8263598.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5680
                     /locus_tag="IVZ90_002605"
     CDS             4829..>5680
                     /locus_tag="IVZ90_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP8263599.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1953 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa
//
LOCUS       DACDBG010000122         5194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDBG010000122 DACDBG010000000
VERSION     DACDBG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.123"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            369..557
                     /locus_tag="IVZ90_002606"
     CDS             369..557
                     /locus_tag="IVZ90_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263600.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            547..807
                     /locus_tag="IVZ90_002607"
     CDS             547..807
                     /locus_tag="IVZ90_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263601.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            848..1075
                     /locus_tag="IVZ90_002608"
     CDS             848..1075
                     /locus_tag="IVZ90_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263602.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1140..1712
                     /locus_tag="IVZ90_002609"
     CDS             1140..1712
                     /locus_tag="IVZ90_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263603.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1713..2024
                     /locus_tag="IVZ90_002610"
     CDS             1713..2024
                     /locus_tag="IVZ90_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8263604.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2015..2275
                     /locus_tag="IVZ90_002611"
     CDS             2015..2275
                     /locus_tag="IVZ90_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263605.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2436..2795
                     /locus_tag="IVZ90_002612"
     CDS             2436..2795
                     /locus_tag="IVZ90_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263606.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2792..4435
                     /locus_tag="IVZ90_002613"
     CDS             2792..4435
                     /locus_tag="IVZ90_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8263607.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4470..>5194
                     /locus_tag="IVZ90_002614"
     CDS             4470..>5194
                     /locus_tag="IVZ90_002614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263608.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDD"
BASE COUNT         1854 a          719 c         1089 g         1532 t
ORIGIN      
        1 tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat tatgtcactg
       61 tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt tcgattcctt
      121 gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
      181 tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta gattgagttt
      241 tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa acgtccctgc
      301 ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa aagagaggat
      361 tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat ttttatgaag
      421 tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg aaggctacgg
      481 gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg cttgcggagg
      541 tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg tgaaagaatt
      601 gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt catagtctgg
      661 tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa aggagcaccg
      721 tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac ttatctcaaa
      781 aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac ataaaggagg
      841 ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc gaaccattgg
      901 tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga
      961 aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat attgcggtca
     1021 tggctattgg aaagacgagt atatccaata caacattgag aggtacatca aatgaatgat
     1081 aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg aggaaaacta
     1141 tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc aatctagctt
     1201 acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct ttagatgcca
     1261 ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct gttcttagtg
     1321 ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt actggatatg
     1381 agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag atcaaaaaga
     1441 ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa atcaaactaa
     1501 gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat gagatgccga
     1561 taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc caagtaccaa
     1621 aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag tacttagata
     1681 gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt aaacaagcaa
     1741 gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc ttagagcgag
     1801 atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa catgattcaa
     1861 ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct ctagatataa
     1921 acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt atgaaatatc
     1981 gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa cagcaaacag
     2041 agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg ataagttggt
     2101 tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat ctatcaacaa
     2161 tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg tagacattga
     2221 ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg gctgataaat
     2281 cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt tgagaagaaa
     2341 taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg ctattttcac
     2401 acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa gagagaaata
     2461 ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt gataaggata
     2521 aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt cttcttgatg
     2581 aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag tcagaaaatc
     2641 aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga gaccaagcat
     2701 atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc gaacctacaa
     2761 aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat tctattttga
     2821 tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa gaattcagtt
     2881 aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg atgaagaaat
     2941 gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta aatatcaaaa
     3001 atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt tttttgatga
     3061 aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag caagagactc
     3121 attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta cagcgaattc
     3181 tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa tgcgcaaact
     3241 ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta attcgatttt
     3301 tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct gcttagaatt
     3361 tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta gtgggctagg
     3421 gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta gagaaggttt
     3481 ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca agaatgatcg
     3541 cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc ctaaaatgtg
     3601 gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc tgttttctac
     3661 agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg aatttgttgt
     3721 taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc atgaaaaatt
     3781 aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg ctggatttga
     3841 ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg atgataaatt
     3901 tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca aactgaaagc
     3961 gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc catcaattga
     4021 tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa tagaaatcgt
     4081 ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag aatcatataa
     4141 ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta ttattgaaga
     4201 tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt ggtatacaaa
     4261 taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa aagagccagt
     4321 aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac gagaagaaat
     4381 aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat tttaaggtat
     4441 actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta tttgttacaa
     4501 tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt actgagtatt
     4561 taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta agtaggctag
     4621 aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg gtatttgtga
     4681 atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa gtaaagcaag
     4741 ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat cgtgagttct
     4801 attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa caagctgtta
     4861 ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac tatgcattta
     4921 cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca ggaccgctga
     4981 agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg atggggaatt
     5041 atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta gctatattga
     5101 atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg tttcaccgaa
     5161 tagttaaaac attaaatgag tattatgacg atta
//
LOCUS       DACDBG010000123         5187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDBG010000123 DACDBG010000000
VERSION     DACDBG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.124"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..609)
                     /locus_tag="IVZ90_002615"
     CDS             complement(<1..609)
                     /locus_tag="IVZ90_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HAP8263609.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSK"
     gene            complement(578..2731)
                     /locus_tag="IVZ90_002616"
     CDS             complement(578..2731)
                     /locus_tag="IVZ90_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8263610.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2989..3225
                     /locus_tag="IVZ90_002617"
     CDS             2989..3225
                     /locus_tag="IVZ90_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8263611.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3244..3459
                     /locus_tag="IVZ90_002618"
     CDS             3244..3459
                     /locus_tag="IVZ90_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8263612.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(4162..4914)
                     /locus_tag="IVZ90_002619"
     CDS             complement(4162..4914)
                     /locus_tag="IVZ90_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8263613.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(4929..>5187)
                     /locus_tag="IVZ90_002620"
     CDS             complement(4929..>5187)
                     /locus_tag="IVZ90_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263614.1"
                     /translation="IESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITT
                     KKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         1701 a          884 c          793 g         1809 t
ORIGIN      
        1 tttactatct gaactctcaa tttgtttttc tagttgtctt tttgtctttt cgtatacttc
       61 taattgcttt tgatcattta attcactttg cttcaaagcg tagcggagtg attctttttc
      121 tgacaacatg ctctttaatt gattactgta gccaaaactg tcatccgttg aaaacaaatt
      181 ttttccttta cttatacttt caataaatag ctgagccatc tttatttgtt gagtaatacg
      241 ttggttttct tgttctatct gagccttttc attctctatt aaagaacaat ccaatctaac
      301 caacacatcg tccttatgaa caaattgatt ttcttttagg aagttttcca ttattttagc
      361 ttctaatggc acttgtaact tctctatttt ttcggggttc aattgagccg ttgtccgaat
      421 aacaacctct tttttagcaa aggttaaaaa caatccaata atacataaaa aaagaattac
      481 agggtataac acccaacggt agaagctatg atgttgttta gtatagatac tagaatgttc
      541 gaaccaatta ttattctgca taaattgttt ccctctccta gatttcccat aatttttgat
      601 agataccatc ggcaaaacga agttcatcgt gcgttccttt ttctaccaaa tgcccttcat
      661 ggagaactaa aatttgatca catgcttttg ctattggtaa atgatgcgca ataaaaataa
      721 tcgttttttc ttttaaattg aggagataat tcataattgc atgttctaac aaggtatcta
      781 atccactagt tgcttcatct aaaattaata tgtccggatt ttttaataaa gcccgtgcta
      841 aggctaatcg ttgtcgttgc cctcctgaaa gattatttcc accttcttcc aacaatgtct
      901 cgtaacgtaa tggctgttga ttaataaatt catttagttg gactatctca caaacttcta
      961 ttatttgttc gaaacttggt gggctatcta aaccgaatat aagattctca atgattgttc
     1021 cactaaagaa aaatgactct tgaggcaaat atgtcactcg atcgcgtaat atctgaaaag
     1081 gaatgtctaa gtgattaatg tttccataat ggatcgatcc ttctgatgct tcataaaaac
     1141 gaacaaataa ctttgcaaga gttgattttc cagaaccact aactccaact atagccgttt
     1201 tactgtgcga aggaattgta catgaaattt cgttcaacgt tggggccttc atgctatacg
     1261 agaatgaaac gttttttaca gtgatatctt gttgaaataa ttgttcagaa agcatatatt
     1321 caaaagactg gttcgatttt tctgaatcaa tcgcgaaaat ttcatttaaa cgattatttg
     1381 caacttgagc cgtttgcatt ttcacttgta aattaataat gttttgtaaa ggatctgtca
     1441 aaaatactag taaagcattg tatgtgataa gttgacctag actaattcta ttatccataa
     1501 caaaataaga acctagccat aaaattaatg cactgctaat taattgaatc aaatgtttaa
     1561 ttccttgttg aatattatca agcgtaaccg tacgaaatga ttttttcatt aatttaacaa
     1621 attcatggtc tatacggtta tatactttct cttcaccgct atatgcttta attgtttcta
     1681 ttcctttaag actttctatg atactagaat ttacagctgc accggctgcc atttcttccc
     1741 tgttcgattt ttcatagctt ttcacgaaag cgaatattgc aactacataa aaaggcaaag
     1801 agcatagagt gattaaaaac aacgtactat tctgaatagc taacgtaacc ccaacaagca
     1861 acaccatacc aatatctaaa aatattgata acgtagcact tgctaaagca tcaataatct
     1921 tactggcatc aagaaaacga gaaataattt cccctgattt tctcgtagca aaaaaattca
     1981 ttggtaaagt taacacatgt ttgaaatact gaagcataat tgccatactc attcgctgtc
     2041 ctaaaataat aagaagataa ctacgactat attcaaacaa tacccgaaat aaatagacag
     2101 cgatcaagcc aatggaaatt atatttaaag ttgaacgtgc ttgattaggg atgaaaaaat
     2161 caagaatacc ttgaaaataa tatgagcttc ctatgccaaa aaacgtaatg aaaatcgatg
     2221 ctaaaacgat gtggaatact aatccttttt gtttccaaat tataggcaaa aaagaactta
     2281 atccagctac tttttcttta ataggaatat aagtttctct aggcataggt aataaaagaa
     2341 tacctgtcca ttctttttga aacgcttcaa tcgtttttct attttttcca ttagcgggat
     2401 ctgcaatcca tagtacgttt cccttcgttt tataaattac aacgtagtgc atatagctgt
     2461 tatcaaccaa tatatgtgca attaatggta agataagatc tctttctttc cagatctcac
     2521 tatctgcttg aatagccata caatcaaaag acaatttttc aaatgtcttt ttcaatccaa
     2581 atgctgaagt accctctaaa tcagtcccag ataggtcccg taatttatga atcgggatat
     2641 cagttttaaa atattttaaa atcatcgaaa ggcaagcaac accacaatct ttttcatctt
     2701 gttgctgcac aaaaggaaat gattttatca cttatctaac acaactcctt ctaaaaaatt
     2761 tttatagaaa aattctcaat atagctttaa tacagtgaaa atattgtctc ataattccac
     2821 aaaaactttg aaaacagttc ttgaacacta actttttcat tcctgaatca aatttatttt
     2881 ggaaatttta ctattcaaga ataaacttga taagtttcag gaagattttt actgttaatc
     2941 aaatacgtat gttacagtag caccgtaaaa aatattattt aaagaagcgt gtttaaacat
     3001 gcaaaatgta aaggaactaa gtattgtgga aatgcacaaa ctgttggtgg aggcaataat
     3061 gcttggggaa aattggggca agttgtaggt ggacttacca caggggcagt aggtggtgca
     3121 ggtttaggaa cagcaatttg cggaccagct tgcggtgttg ttggagggtt atatggagct
     3181 gtcgctggag gtgctgcagc tggctgggat gctcgaaaaa aataaaaaag gagaataagt
     3241 agtatgcata ttaaaaacac aaaaacaaca tttatattat cttcagaaga gttgaaaaat
     3301 attcaaggtg ggagtgccgt gggagtttta ggcactactt tttctggggc aacagcagga
     3361 gtaaaacttt gttcagccgg aggaccttat gcaatagctg cttgtggtgt aggtggagct
     3421 ttattaggtg ctggattcag catgtggacc ggtgcgtaaa caattaaaaa atatcaaacc
     3481 tcaagattgg tttgtgtcga ttacgaacat aggtatgttc tttatctttt caaaagtttg
     3541 gaatggacag aactttttac taaagcttct tgtgctagtt tttataatta tattaaattt
     3601 ttatctttta ttaaagtcag ataaaaattc tttggaaatg taatggtaga gatattttta
     3661 caaaaggact tgcagcgctg tagttggtgc tgcaggagga cctatttcgg ccgttacagg
     3721 aggtattggt aatgtacttg tcggatgtgc attaaagcct gttcctgcaa gttaaattta
     3781 taaaaacagg aaagtaatta tctcaaatcc tatcaagtac aatcatagga ttgcacttga
     3841 caggacaaat tttttaacta aaaaatgaaa aacactttct aaaaattagt agaatttgcc
     3901 aaatctttcg catcttccac catacattta attttaattc ccctttatgg tacttaataa
     3961 aaacaagaaa agaactattc atttggccct tttttggttc cagaccatag aaatattcct
     4021 ccagtaacta atattgctgt tatgcacgta actaataaca gctataaaaa ttggtacaac
     4081 tttaatagga ttcagaaaaa tttgcactag tgaaggaatc tattttatta gaaaaatgca
     4141 atctccctaa agaaaaaacc actaaacagt gctttctttc aacttctcct taattttttg
     4201 tgctttccct aaagtaaagt ttctggccaa ctctgaatca aaatatagtg aacgtttttt
     4261 aaaatctatt tctttaacgt taaagatatt tactaaacac gctcgactaa ttcttgttaa
     4321 cataggatat ttcttttcaa gctcactaat tcttccataa aattcgtact gcccatcttt
     4381 agtacacaat gataatctat gtggtaattt tgaagcttct acaaaataaa tgtcatttat
     4441 atcaaaagta aatgtttgag aacctatgga aaaaacaaat gcttgattcg taacctgttt
     4501 tgttgcatct atcctttctt gcgctaataa caataactca acaatctcgt ttcgatactc
     4561 atctagtgtt tgatcttttg ttacaaatcc taacgtttca actcttcttt ttattgttac
     4621 gggtaacatc tcatcatgag tagttgtaaa aatgattttt gcttgaacat catattttct
     4681 gattacttct gctaattcta taccgttaac ttcatgattt aaatcaatat ctaagaaata
     4741 tattccttgc tttggtcgga attgtttgag gtatttttta acttctaacg gactttgtgt
     4801 tttcaatact atcttaaata ctttatcgtg aaaaagaatg aaattatcaa taattcgttc
     4861 taactgattt aattgtataa tttgatcttc acataatatt attggataac tcataaatcc
     4921 tccttccctc aaaacaacaa aataacttga gtagtaaatt gatacggatc gttttgatag
     4981 ttcacaaaca tattgggatt ctttttattg atttcctcta tactagataa acctaaccct
     5041 tgatgtccct tttttgttgt tattcctttt ttcattagag tatctataga gagtagtatc
     5101 tctctacaac tattcgatat cgaaatttct atctgtttat catctcggta aatagctaga
     5161 gacagttctc tatcttgact ctctata
//
LOCUS       DACDBG010000124         5043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDBG010000124 DACDBG010000000
VERSION     DACDBG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.125"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IVZ90_002621"
     CDS             complement(406..780)
                     /locus_tag="IVZ90_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8263615.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IVZ90_002622"
     CDS             complement(803..2203)
                     /locus_tag="IVZ90_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8263616.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IVZ90_002623"
     CDS             complement(2207..2617)
                     /locus_tag="IVZ90_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8263617.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IVZ90_002624"
     CDS             complement(2635..3126)
                     /locus_tag="IVZ90_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8263618.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IVZ90_002625"
     CDS             complement(3136..3927)
                     /locus_tag="IVZ90_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8263619.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IVZ90_002626"
     CDS             complement(3920..4693)
                     /locus_tag="IVZ90_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8263620.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5043)
                     /locus_tag="IVZ90_002627"
     CDS             complement(4849..>5043)
                     /locus_tag="IVZ90_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8263621.1"
                     /translation="SGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYC
                     IEIPKGEYELIYTIFEQTIF"
BASE COUNT         1605 a         1027 c          662 g         1749 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 aga
//
LOCUS       DACDBG010000125         5010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDBG010000125 DACDBG010000000
VERSION     DACDBG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.126"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(314..712)
                     /locus_tag="IVZ90_002628"
     CDS             complement(314..712)
                     /locus_tag="IVZ90_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263622.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            928..1983
                     /locus_tag="IVZ90_002629"
     CDS             928..1983
                     /locus_tag="IVZ90_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8263623.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2003..2356
                     /locus_tag="IVZ90_002630"
     CDS             2003..2356
                     /locus_tag="IVZ90_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263624.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2538..2834)
                     /locus_tag="IVZ90_002631"
     CDS             complement(2538..2834)
                     /locus_tag="IVZ90_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263625.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2838..3656)
                     /locus_tag="IVZ90_002632"
     CDS             complement(2838..3656)
                     /locus_tag="IVZ90_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8263626.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3882..4103
                     /locus_tag="IVZ90_002633"
     CDS             3882..4103
                     /locus_tag="IVZ90_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8263627.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4105..4371
                     /locus_tag="IVZ90_002634"
     CDS             4105..4371
                     /locus_tag="IVZ90_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8263628.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4484..>5010
                     /locus_tag="IVZ90_002635"
     CDS             4484..>5010
                     /locus_tag="IVZ90_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263629.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDV"
BASE COUNT         1736 a          870 c          776 g         1628 t
ORIGIN      
        1 tcctttaaaa agcacttatt ttaatataat gttattttat aatcttatta caagctgatt
       61 gttaggcgtt gtcctttcat tacatttatt aacggttatt ttattgttta atttattaat
      121 tatttttgat ctaaaactta gcttaacaaa taaataatta accaaccgcc agataaaaat
      181 gcgatcattg ctaatacata agcaaagata aaaatatgtt cgatcagctt aaaccctgat
      241 gcgattaagc agaccaagta aaatagaaat tctcttacag ataatggtaa ttttctaact
      301 agcatactca taattaacac cccaaagtaa tattgtattt tgaacatttt acatccaata
      361 cagcatcaat caaccattgc caatcaaaaa tacaaggctt cattgaagta tgtttttcag
      421 atgaaaagat ataatataat ttcttcggaa taatattttc agttgttggg atcaagctaa
      481 atctaatacc gtctaactca tagctaccag ctttctctcc attaaaaatt tccttagcac
      541 tttcttctaa agaatcaaaa tatttttcga cagcaatctt tgaagctttt actattgaag
      601 catttccctt ttcgataaaa tctgcttttg ttttaccgac aataagtaat tcttgatcag
      661 taaaatcttc taaaataatg gtagtgtaat tcttatttct tctcaatcgc atcaggatca
      721 ctccttaata taatttatgt ttttataata tcataataag ataaatttgc aagcatttat
      781 ttacaccttt ttatttacac tttattataa ttaattagac ttttatgatt gcattttttt
      841 cttataaaca gttgcaacta atagtaaata ggtgtaaact attttcgtac cgaaatacca
      901 taaacacaaa cagaaagagg tcataccatg aaagaaccat tattgttttc cgttgactta
      961 gggaataaat cagtaaaaga aatgagtaca cacgtttctc cactctcact tccctctcgt
     1021 attttgaaca aaaatatcat taattcagga cgtttttcta atataggttc ctcattaaaa
     1081 cgatcactaa acgtttctga atatagccta gtaaacacta ctgaaagcta ttactttggg
     1141 aaggatatac acgagctagg aaaagatgac tacgtgatag aatcactagg ttttgggatc
     1201 agcagataca agcaggaaac ctaccaaaat atcctatctt tctctttagc taacctagca
     1261 cgtttagaag atcaatctga tgacgtttta accgctaatc tagttttagg tctgccttca
     1321 tccgactata ccgaaacaat cgtcaaatac gtgatgcaac aagccacacg acaacattca
     1381 gttgaagttg acggtaaagc ttataatata aacgtagata ccgtttgggt actgccacag
     1441 ccgattggaa cactatataa cctcctactt gctgatgatg gtagtgtaca agatgaaaca
     1501 ttgatcagtc agaagatcgg ttttgttgat attggtggtg gcacgaaact ttttgatgtt
     1561 gtccaaaatt ttgaaattga cgaaaacaac cgtgaacaaa aggataccgg tatttttact
     1621 ttatataatc gtatcgtaaa tatgcttgac cttccgatca gtcctaatgc tttccaactt
     1681 gaaaagatta tccgagaagg caaagaaaaa ggcgtatatc tatttaaacc aagcggaaat
     1741 gaaagttttg atatttcaga agtagttgaa agagaaataa aaacatacac caatatggtt
     1801 gttgcagaag ttttagctac attcaaaaac ttcacaacct tagataaagt tgtctttact
     1861 ggtggtgggg cttctattat cgacaaacaa cttgtaaaag ataaaattcc gcataccttg
     1921 tttgttgaag acagtgaatt ttctaatgta aaaggctttt acaagtttgg tttaactcaa
     1981 taaaacagcg aaggagatca acatgaataa gggaaaatca gagcgttatg tagttataat
     2041 caatagagaa aaggttagcg agttaccttt agcagagttt cttgaatctg aatatgaaac
     2101 actaggcgtt aaatactctc agacgatcaa aaaagctttg aatctatatc ggcgtgtaaa
     2161 gaacaatgaa gttacaatta ttgaagcagg caacgataga aacgatagca aagtagctga
     2221 agtaaagaat aaaccaactg atcataaccc tattcaacaa gcgcatcctt caacggttac
     2281 atccgatcat tcagcaaaga aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa
     2341 caaagactta ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta
     2401 caaatacaaa cataaacata tacgtataca tgtatataaa catgtatacg tatatatcca
     2461 taaacacgta tacgaaattt agttaagcta tacctaaccg cttattccaa taaaaaaagt
     2521 gatcagcttt aaactgatca ctcaccaaaa acttcatcat acaagttatc tatcatttca
     2581 ccaagtaaag ctgaatctga tcgaattcct aacttttcgc tgattttaga tagtttcttt
     2641 ctttgtgatg gttgaagcgt gaattgataa tttttcttct tttcagaact atcacttttt
     2701 agtttagcta ccaaagaact tgttgtttct ttaggtgttt gtttaggttc ttcaactgct
     2761 tcaaccactt caggaacttc ttctacttca gcttcagcga attttttaaa tctattttct
     2821 ttcacttcaa tcactcctta ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac
     2881 gtgctttcaa agaaatcctt attcttacga tagatattct tatccttagc gaactcagca
     2941 atattcttac cttctaagct agttgcatta agcaattcct tctcttgtac atatccaaga
     3001 taattagata acgttggtac caattctagg aactcacgac ttgaattagt attatgtttg
     3061 atcctgctac cgatcgtagc gtatttagtt tccatcagtg tttcaccagt cttaatatca
     3121 atcaactgtt ttgctagtgc atcaatatct ttaccaagct taagcaaagc ttcaaaacca
     3181 tctttagatg gatcagaaat gcctacgaca agatcagata cagcccaagc attttgagct
     3241 agtagacctg catcattatg agtatcaatc agaattagat catacttctc tttaagctgt
     3301 tcatagttaa caacgatcca tctaaacagt agggttctgt tgttaggcat atcacgcatt
     3361 tgcatctcaa cttgttttaa atcttcacct gctactaaaa tatccatgtt ctcatttata
     3421 gctaaaggtt cagctgatcc atgaagatat aaatgattga ttgtattctc tcttttcgct
     3481 ttactgaaag catcaggaaa tcgttttgat aggttttgcg acttatcttc atcaatatac
     3541 agcaccttca tacctaaatt ttcgacagca tatccaccta gattgtagat tgttgttgtt
     3601 ttacctgttc cacctttttc aatgtttcca gtaatgattg acaacttctt ttccatgatt
     3661 aaacctccct tattaatctt ttgtaactcc attatacact ataaacacgt ttatgtatat
     3721 atttatatac ataaacgtgt ttatatttat atttgctttt atattctaca acataaacat
     3781 atatatgtat atgtttatgt ttatgtatat attcaaatat ataactattg catttgtcat
     3841 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     3901 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     3961 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gactttgcca
     4021 cctacaagca actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     4081 tgctaaaaga gttaggctta taagatgact taccaagtaa gatatgaacg tgaagctcaa
     4141 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     4201 aatcttgtag gcactgatga tccaagaaag catggtaaag gtctaacagc taataaatca
     4261 ggctactggc gatatagggt aggtgcatac cgtataatag ctgatattag cgagcatgaa
     4321 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     4381 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     4441 acctaaaaaa gaacaatatc ctcttgatat tatacacacc agtgtgtata atataagtgt
     4501 aaacaagcta attaactttc aaaaggagcg attttttatg ggatggtatg gtgattattc
     4561 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     4621 aggtaccaaa gtaacgaaga cctatggaat tatccttttc aaagatgcag aaggaaccta
     4681 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     4741 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     4801 agaaaaaatg gttaagctac aactagatag aattaaagaa gaaagcaata aaccaaaact
     4861 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     4921 ctatcagtat aaactcaaac gtagctttat ttttcgacta caagatggat cactcactcg
     4981 gttcactcga ctatcagcag gagatgttaa
//
LOCUS       DACDBG010000126         5009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDBG010000126 DACDBG010000000
VERSION     DACDBG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.127"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IVZ90_002636"
     CDS             <1..395
                     /locus_tag="IVZ90_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263630.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IVZ90_002637"
     CDS             398..1147
                     /locus_tag="IVZ90_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263631.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IVZ90_002638"
     CDS             1159..1428
                     /locus_tag="IVZ90_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263632.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IVZ90_002639"
     CDS             1590..1892
                     /locus_tag="IVZ90_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263633.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IVZ90_002640"
     CDS             1889..2050
                     /locus_tag="IVZ90_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8263634.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IVZ90_002641"
     CDS             2047..2352
                     /locus_tag="IVZ90_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263635.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IVZ90_002642"
     CDS             2352..2708
                     /locus_tag="IVZ90_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8263636.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IVZ90_002643"
     CDS             2668..2913
                     /locus_tag="IVZ90_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263637.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IVZ90_002644"
     CDS             2910..3329
                     /locus_tag="IVZ90_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8263638.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IVZ90_002645"
     CDS             3326..3883
                     /locus_tag="IVZ90_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8263639.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IVZ90_002646"
     CDS             3880..4176
                     /locus_tag="IVZ90_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263640.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IVZ90_002647"
     CDS             4253..4666
                     /locus_tag="IVZ90_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8263641.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1864 a          774 c         1140 g         1231 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagt
//
LOCUS       DACDBG010000127         4936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDBG010000127 DACDBG010000000
VERSION     DACDBG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.128"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IVZ90_002648"
     CDS             complement(26..466)
                     /locus_tag="IVZ90_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8263642.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IVZ90_002649"
     CDS             626..1132
                     /locus_tag="IVZ90_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8263643.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IVZ90_002650"
     CDS             1300..3213
                     /locus_tag="IVZ90_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8263644.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IVZ90_002651"
     CDS             3218..4219
                     /locus_tag="IVZ90_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8263645.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IVZ90_002652"
     CDS             complement(4421..4627)
                     /locus_tag="IVZ90_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8263646.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1822 a          756 c          997 g         1361 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaat
//
LOCUS       DACDBG010000128         4828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDBG010000128 DACDBG010000000
VERSION     DACDBG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.130"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            222..473
                     /locus_tag="IVZ90_002653"
                     /pseudo
     CDS             222..473
                     /locus_tag="IVZ90_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            566..1360
                     /locus_tag="IVZ90_002654"
                     /note="aph(3')-IIIa"
     CDS             566..1360
                     /locus_tag="IVZ90_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8263647.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1770..1881
                     /locus_tag="IVZ90_002655"
                     /pseudo
     CDS             1770..1881
                     /locus_tag="IVZ90_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1902..1985
                     /locus_tag="IVZ90_002656"
     CDS             1902..1985
                     /locus_tag="IVZ90_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8263648.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            2110..2847
                     /gene="erm(B)"
                     /locus_tag="IVZ90_002657"
     CDS             2110..2847
                     /gene="erm(B)"
                     /locus_tag="IVZ90_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8263649.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(3110..3973)
                     /locus_tag="IVZ90_002658"
     CDS             complement(3110..3973)
                     /locus_tag="IVZ90_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8263650.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3975..4247)
                     /locus_tag="IVZ90_002659"
     CDS             complement(3975..4247)
                     /locus_tag="IVZ90_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8263651.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4264..4479)
                     /locus_tag="IVZ90_002660"
     CDS             complement(4264..4479)
                     /locus_tag="IVZ90_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8263652.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4571..>4828)
                     /locus_tag="IVZ90_002661"
     CDS             complement(4571..>4828)
                     /locus_tag="IVZ90_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8263653.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1486 a          812 c          884 g         1646 t
ORIGIN      
        1 tttattttgt ggtgatttaa tcatactaga gaacgatatg ttttttaata cctaaaatga
       61 aaatggggct gaagaatcaa ttctgattca tcagtcccat aaattataga gcccatttct
      121 tgggagtttt gagactacta tatgcgtttt tttgttgatt taccaacatg tttagacatt
      181 ttcctctagg aaaaaattaa ctagcaccac gggttatctt ttatatagaa gatatcgccg
      241 tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat
      301 gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag
      361 cttgtaaatt ctatcataat tgtggtttca aaatcggctc cgtcgatact atgttatacg
      421 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
      481 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
      541 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
      601 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
      661 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
      721 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
      781 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
      841 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
      901 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
      961 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
     1021 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
     1081 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     1141 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     1201 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     1261 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     1321 gaaaataaaa tattatattt tactggatga attgttttag tacctagatt tagatgtcta
     1381 aaaagcttta actacaagct ttttagacat ctaatctttt ctgaagtaca tccgcaactg
     1441 tccatactct gatgttttat atcttttcta aaagttcgct agataggggt cccgagcgcc
     1501 tacgaggaat ttgtatcgat aagaaataga tttaaaaatt tcgctgttat tttgtacatt
     1561 taacttgacg gtgacatctc tctattgtga gttattagtg gtacagtttt caaccgtttt
     1621 aattataaaa aagtggtgca tttttaaatt ggcacaaaca ggtaacggtt attgcaggtg
     1681 tatttcttat ctatgggttt aacatggatt ttatcattaa aatcatgagt attgtccgag
     1741 agtgattggt cttgcgtatg gttaacccta aagttatgga aataagactt agaagcaaac
     1801 ttaagagtgt gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa
     1861 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
     1921 gttatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
     1981 aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
     2041 ctaaaattac aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg
     2101 agtgataaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
     2161 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
     2221 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
     2281 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
     2341 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
     2401 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
     2461 cgtgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
     2521 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
     2581 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
     2641 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
     2701 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
     2761 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
     2821 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
     2881 acacgttact aaagggaatg tagataaatt attaggtata gggcacctct aataactacc
     2941 aattaattca cctcaaaatg aaattagaaa aaaacagcga aatttttaaa tctatttctt
     3001 atcgatacaa attcctcgta ggcgctcggg accccaacat gaggaaatgt ttttataaaa
     3061 aaagagtagt taccgttttt tggtaactac tcttttcctt ttttaaggtt taaatacctg
     3121 gaagtttagg tgttttgggt attggtggtg taggtggctg caagctttcc aatttttgtt
     3181 gaattgcttt aaattcaggt gtctcttggt gtttatttag aaccattttt tgctctattc
     3241 gttctaaagt cggttgaatt tctttccctg atactttacg attcaattct ttttctaagg
     3301 tttcttttgg actaatagaa ggtgtttcca aacttgaata gagttttact ccttctctgt
     3361 tatatagcct tatatcgcta aataagcccg ttttatgaag ggtttctaaa ttggtcggta
     3421 agtttttgac aacaatatca tgcgcttgtt ttggtgttgc cctggctgtc attggatcat
     3481 ctgcatacat ggtttcatat cgttcaattg ttcctaaata tgagttgatt ttaggtactg
     3541 ccatgacata catttttgtt tcataacctt tggcttgaag cattgttgcg gtttgaatag
     3601 gaacgtctgt tgttcgtcct gtaccttcga tcaccaaatt atacccttga tcgctcaaac
     3661 ggcttatgat cgcttctgtc atgcgattag aataaggggt aacgtgtttt actacgtctt
     3721 tttcataaag tttcactagt tcatcaaaat tagggtgctg ttgtttaaag gtatcattat
     3781 caatgacaat aacattccct tgtgtttctt caaaaattgc tgatcgcaaa ctggttttcc
     3841 ctgaccctgg ttgcccacca agtaaaaaag cggttggcga ttcaaccgct ttttttcctt
     3901 gaatcaattc ttctaaatta tcatttaagc gattctcaaa ttgtttgtca gtaaaattga
     3961 ctatatttgc catattaagc cactttctct ttattcaaaa cttgttttga gtaacgattc
     4021 attgaccgcc aatactcatg aacggcttgt aattcttcta aagaataatc aaaaagattt
     4081 acacgttttg caagctttaa ttggtttaat aaattgactt ccaataattg agagtcattt
     4141 ttatttaatt catggttcaa aacatagttt aatactcgat tataaacgct tgccgataat
     4201 tcgttaatga tctctatttc aaatgttttt tcatacgtaa ctgccatttc attttcactc
     4261 cttttaaagt ttgtcaggta aatatttccg tatatattct tctaaggctt gatccataac
     4321 ttcttttacg tttccgccat tctttgctgt ttcgattttt atgatatggt gcaagtcagc
     4381 acgaacacga accgtcttat ctcccattat atcttttttt gcactgattg gtgtatcatt
     4441 tcgttttgct ttttgtgcgc ctaaatttcc cacaatcact cacttctttc tatttcttct
     4501 tactcttatt ttatcatcaa caatcacaaa tcacaagtga tttgtgatta atcacttgtg
     4561 atttgtgatt ctattctttt tcgttttcta attgaataat tcgctcaagc atttcaaaaa
     4621 atacgttctt atacatggat aaaacttttt tgtcatagcc tttatgctct gtaatgccgt
     4681 ttttagacca ggtacttact ttattacttc gcttgataat attttggaaa accaagttat
     4741 cttctttatg ttgcttatac agttcttcca ggtttgattt tatcgttgcg ctgtccgtat
     4801 caactaaata aggaacaaaa ccgatcat
//
LOCUS       DACDBG010000129         4737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDBG010000129 DACDBG010000000
VERSION     DACDBG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.131"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            304..1281
                     /locus_tag="IVZ90_002662"
     CDS             304..1281
                     /locus_tag="IVZ90_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8263654.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            1291..1788
                     /locus_tag="IVZ90_002663"
     CDS             1291..1788
                     /locus_tag="IVZ90_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263655.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            2164..2445
                     /locus_tag="IVZ90_002664"
     CDS             2164..2445
                     /locus_tag="IVZ90_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263656.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            2447..2863
                     /locus_tag="IVZ90_002665"
     CDS             2447..2863
                     /locus_tag="IVZ90_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263657.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            3516..3941
                     /locus_tag="IVZ90_002666"
     CDS             3516..3941
                     /locus_tag="IVZ90_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263658.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
BASE COUNT         1744 a          807 c          773 g         1413 t
ORIGIN      
        1 gtttgaggaa aaaagcagga gttttttagg cgggaaattt tgcctcctct tttataatct
       61 ctttttaaac ctctttttaa acctctttac cgtcggctgt atgcttactc tcccaaggtg
      121 tgcagaatct tttgggggtc tttttgtctg tttttagggg tctttttgtc tgtttttagg
      181 ggtctttttg tctgtttttg ggggtctttt tgtctgtttt taggggtctt taattagggg
      241 cttgacccct attagaaagc tctttataat tttgttaagt agttaatctt gaaggaggat
      301 tttatggcaa atgaaattgt gaaataccac catgaattaa atacgattcc tttacgcaaa
      361 tttacgtcag tagaaatgaa tttattcttc tctatagtat ctagaatgcg tgacgtaggc
      421 gataaaaaag tacaatttac attcgaacaa ttaaaagatt taagcaatta taaagcaact
      481 gcaaatgttc gattcataga tgatttagaa acaacgtatg acaaattaat ggatttacgt
      541 tttggtagaa gaagtgctga cggtttgcaa cgtgaacgat ttgttttatt taatcaattt
      601 aaaatcgatg gaaaagctga tattcctttt gctgaaatac aagtccatga gaaagcctta
      661 cccttactta ataatcttga agaatgggtt cgatattctc tccaacaatt caatgaatta
      721 gaaagcagtt attccaaaac catgtttaga ttattaaaac aatacagaac caaaggaatt
      781 gcctattttt caaaagaaga ctttcatgaa ttacttgata tccctaaaag ttatagagaa
      841 agtgatataa ataaaaaaat tataaagcct attcgccaag aactaaccgc aatcttcaaa
      901 ggtctgacaa tcaaaaagaa atacggaaaa ggaagaggaa aacctgtcat tggctatcaa
      961 ttcacgttta aacctgaaat aaaaaaagca gacgacttta ataaacacgc accaacaaaa
     1021 gaagaacaaa ataaagcttt agaactagtt aatcaacaaa tttactctga aaaagacccc
     1081 acttcattac aagaaaaaat caaagaacag aaacgaaaat tagaactttt acaacaatta
     1141 gaagagctag aaagaaaaca aaataatctg aataaagaaa ataaggagat taacgaacaa
     1201 ataccagata acaattccga tattccccca gaattaaaaa aaggcttact agataatctt
     1261 caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa agaattagca
     1321 gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt acctagtaat
     1381 tttacctaca aaaaaggaaa tattatctac ctgaaaaaag aagcagtaac aacaattaca
     1441 aaggttttat caggtaatga gccgggtaac ttacctagtg aattacctga ttcattacct
     1501 accgattacc tactcaagga gattgaaact aagaataatc atattgaaga aaaagatagg
     1561 caaattgaaa aacttcaact agctttagaa acacaacaaa aattactcga ccagcaacaa
     1621 caactcaccc tgcaagccaa ccaacaaatc gagaaactgc aggaacaact acagttgact
     1681 tatacggaag aatcttcgga aaataaatca acgactcttt ctgaagaaac agaatcgata
     1741 gaaaaacaat caaagacgga gaaaaaatgg tggcaattct ggcaataaaa ccagcaattt
     1801 aaattaataa aaattgctgg ttttattctt ctttttgctt ttgcgagcag agcaacatgg
     1861 gcccatgttg cgatattttg gtggcgcccc aatccccgtt ttttcgttga atcttactcg
     1921 gactgcactc gttttacagc tgatttttct gaattttgaa agagcataag gaaacgtggt
     1981 atcactttct tacgaaaggt gctagataag ctgtgcctat cacgtttctc agaaaaaaat
     2041 ttgctatcac ttttttgcca ttgtggctag gctatgtata gtgttttggc gaaaaatagc
     2101 tatacaaata ctatatttgt atgatataca taaattatat actgatacga aaggaaaatt
     2161 tccatggaca ttttagttcg aaatatcaat cccaaaatta ttacaagaat agataaacgt
     2221 tgtcaggaac taacaaccaa aacgggcaaa aaatggagtc gaaatcaata cctaaaaatt
     2281 ttaatcgaaa acgattttga ccacgaactt atgaattaca aaaaagatca attcgattta
     2341 ttattcgaaa aatttattgc cattcaagca gaaaatacga acatactaaa tgactatgtt
     2401 cacacaaaca accaactcat tgaaacactt atcggaggaa tataacatga gaaaaacttc
     2461 tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac gttttagatt ccatcatcga
     2521 aaaaaatcca gacgtcaaca attattcaga agccattcgc tttttatgta tgagttatga
     2581 acctgctacc aatacaacca caaccagtaa ccaagcaaaa ctcaatgcca tgggaaaaga
     2641 aatttctatc atgacagaaa tcatggcaga atttgctgat ttatatctaa aagatactgg
     2701 tgttcttatc ggtacaaatt ctaaagtata tcaagaagtg aaagaaaaaa ttgaaaatca
     2761 actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa aaagaaacca atcaaacctt
     2821 tacttctgga aattttgacc caacaaactt tctaaaaaga taaaaaaaga aaccgaaaaa
     2881 taaaaaaatt cctcgatttc tttttaggga tcggtcttcc agcaagattt gcaagttggt
     2941 accaacttgc gaaaaaaaaa agaaaaatcc gcttctgcga attcttcttt tttcattttg
     3001 gggcgctgcc ctaatcccta agtcccggtt attcaatagc atggaaactc aaagaataag
     3061 acgttccctg gaataaaaaa aaaggctgct cgttcgagca gtccatacat aggaaaatga
     3121 aaaaacgacg tcccttactg agaaacgcta ttaaactttt aacaaatcct ctaaaaaata
     3181 acaagtcttt aaaaagtgac cggtaaaaat gcacaaagaa ctaaaaaaga gaaattgcat
     3241 caaatcaagc attagaagct agtaactagc tagaccaatt gaaaatcaca aagtgagcgc
     3301 ctgtatctat tgctacaggc gctacttggc atagtgtaca ttatgtcaac aagaatttaa
     3361 tataacatat ttttctagtt tgttataaca atgccactct aacaaaaatg taagcattat
     3421 ccttattaaa agctccttga caaggactat accttttatt ttatgatttt tacatagaaa
     3481 acgttttctc tattttttgt ttagaggtga tttttatgat ttgcaagaac aagaaaaaaa
     3541 ttattgctat tattgctagt ttctctttga tttttagtac tgcatgtact gtcttattaa
     3601 caaataatgc taatgttgaa gctgcaagct cttcttacaa acataatcat gctggttata
     3661 gatgttacaa aatgagttat tatgtaagtc cttctaaatg caaacaattg gctcgccaaa
     3721 ctaataaact taataccgtc tcagctattg caaactttat tggactatct ggattaacac
     3781 cggggattat atcattagtt ttcggatcag cagttagtgc taataatgta tttgttacag
     3841 ccgctagaag aggaaaaggc gttcaattaa attatattgc ccattttagt aataccacta
     3901 cctataatta tagttctaac caaaattatg ttataaaata attaaaactt taaactagga
     3961 ggaatgcttt atgaaattaa caaaaattgc tgtaatttta gctgttattg ctggcattat
     4021 tttatatttt gtattgcctg ctaatcaatt cttcctttac tttggaatac ctgcgatttt
     4081 aatagtaaat ggcgtaagaa tctttgaaca acgaaaaaaa tgtagctcta cctaatcgtt
     4141 acaaaacaac aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa
     4201 atgctagcct tttttgtatt gtagagcgtg aattttgagg gggattcaag ggggagcttg
     4261 cgcccctttg acaagcggtt atttgcacca aatacctccg tatttggcta gcaatccttc
     4321 taaaaaatac acctatcgat cagagtcaaa acaaatcaat tttcgagtga gcgaatccga
     4381 atttgcaaag ctgaagcttt gcaaatgagt gtgccggctt ccgtaaaaat acatatggct
     4441 ataatttgcg ttttaaggcg ttttaaacgt tctggtatat aattatacca aaacaacttt
     4501 aaaacgcctt agatctaatt ttagaggtct tatagaacat tttagggttg ttagggcttg
     4561 ccctgaccgc ctgtaaggcg ctttgagaac aagataaggg ttttatacta agactgtctt
     4621 aaaacggctt aaacaaacgc tcagaacttt tcaaaggagt tctttatgaa ctgtttttaa
     4681 gcaaaggagc ctttaggcga ctgcgcagta taatgtgaga gcgtagcgat cacacat
//
LOCUS       DACDBG010000130         4701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDBG010000130 DACDBG010000000
VERSION     DACDBG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.132"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            221..655
                     /locus_tag="IVZ90_002667"
     CDS             221..655
                     /locus_tag="IVZ90_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8263659.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IVZ90_002668"
     CDS             678..998
                     /locus_tag="IVZ90_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263660.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IVZ90_002669"
     CDS             1139..1330
                     /locus_tag="IVZ90_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263661.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IVZ90_002670"
     CDS             complement(1401..1658)
                     /locus_tag="IVZ90_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8263662.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IVZ90_002671"
     CDS             1816..2079
                     /locus_tag="IVZ90_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263663.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IVZ90_002672"
     CDS             2103..2291
                     /locus_tag="IVZ90_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263664.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="IVZ90_002673"
     CDS             complement(2374..2559)
                     /locus_tag="IVZ90_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263665.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="IVZ90_002674"
     CDS             2774..3145
                     /locus_tag="IVZ90_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8263666.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="IVZ90_002675"
     tRNA            complement(3295..3367)
                     /locus_tag="IVZ90_002675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="IVZ90_002676"
     CDS             complement(3413..3841)
                     /locus_tag="IVZ90_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263667.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="IVZ90_002677"
     CDS             complement(3901..4659)
                     /locus_tag="IVZ90_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8263668.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1643 a          736 c          918 g         1404 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa a
//
LOCUS       DACDBG010000131         4674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDBG010000131 DACDBG010000000
VERSION     DACDBG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.133"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..658
                     /locus_tag="IVZ90_002678"
     CDS             <1..658
                     /locus_tag="IVZ90_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8263669.1"
                     /translation="KHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFS
                     CVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKF
                     GETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIE
                     RSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW
                     "
     gene            670..1674
                     /gene="neuB"
                     /locus_tag="IVZ90_002679"
     CDS             670..1674
                     /gene="neuB"
                     /locus_tag="IVZ90_002679"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8263670.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1671..2819
                     /gene="neuC"
                     /locus_tag="IVZ90_002680"
     CDS             1671..2819
                     /gene="neuC"
                     /locus_tag="IVZ90_002680"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8263671.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            2792..3469
                     /locus_tag="IVZ90_002681"
     CDS             2792..3469
                     /locus_tag="IVZ90_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8263672.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3459..4601
                     /locus_tag="IVZ90_002682"
     CDS             3459..4601
                     /locus_tag="IVZ90_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8263673.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
BASE COUNT         1729 a          608 c          922 g         1415 t
ORIGIN      
        1 aaaacatttt gtaggtatca gtacagataa agtagtgagt cctactaaca ccatgggtgc
       61 gacaaagtta ttgagtgaaa aattaataat agctgccaat cagtataaag gaaaaacaga
      121 aacaatattt tcttgtgtaa ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc
      181 tctttttagt aaacaaataa aaaacaataa agatattact ttgacagatg ttgatatgac
      241 acgatttatg atgtcaatac cggatgctgc aagattagtt ctgtctgctg ctatgaattc
      301 aaaatttgga gaaacatatg tcttaaaaat gccggcaatc aatattttaa ctttagctca
      361 atcattaata aaaatatata atgatacaca tgatgaaaaa tatagaggtg aaataaaagt
      421 tgtaggtatc aggccaggag aaaaattata tgaagagcta atgactacag acgaaattga
      481 acgctcatat gaaaatgata aaatgtatat cattccacca cactatcatg ctactgacga
      541 actgtacaaa gattatgaaa aaattaaaat cactgcatac tcttcagatg caacagaatt
      601 aatgagtgga caggaattta ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg
      661 agttttatga tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa ccataatgga
      721 agtctagatt tagctttaga aagtattgat aaagctaaag aatgtggagc taacgcggtt
      781 aaatttcaaa catttaaaac gaataagcta gtttccaaaa aggctccaat ggcaaaatat
      841 cagaaggata atcttaaaga ggacagttct caatttgaaa tgctaaaaaa attagaatta
      901 tctcaatctg attttattag aataaaagat tattgtatac aaaataaaat tgattttttg
      961 tctactcctt ttgatgaaga aagtgcagac tttctaaaac aaattggtgt acaaggattt
     1021 aaaattggct caggagattt aacaaattta ccttttctta gaaaaataga tatgtatggg
     1081 ttgccagttt tattatctac aggtatggct gatattcatg aggtcagtga ggctgtagat
     1141 tgtttcatag atagtccagt tactatttta cattgtactt caaattatcc ggctagttct
     1201 gaagatatta atttattggc gattaacaca ttaaaagaaa tgttcgggat acccgttggg
     1261 tactccgatc attcattggg atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag
     1321 gtaattgaga aacattttac cttggataag gaactacctg gtccagatca taaagcttct
     1381 ttagatcctc aggaatttgc agattttgta aagcatatta gaaacactga aatattttta
     1441 ggtgatggtg ttaagcgagc aatgccttct gaacttagta caaaagaagt atcaagaaaa
     1501 agcctagtgg tcactgagaa tttaaatgtg ggagatattc ttacagagaa atctcttaca
     1561 ataaaacgtc ctggaaatgg aatagctcct aagtttttat atcaatatat tggcaaaaaa
     1621 gttaaaagac caataagtgc agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa
     1681 tacttatagt aagtggaaca cgagctgatt atggaatata taaacctgtt gctgagaatt
     1741 tactacaaga taattgggat gtatcatttg ttgtttctgg aatgcattta tcccataaat
     1801 atggatttac taaagaattg atcttagctg acggatttag aattagtgga gaagttcaaa
     1861 gtttgttttt agagaacaat aagggaaaca tggctcggtc tgtggcatta gagataaatg
     1921 gtttcacaaa tatatttgaa caaactagcc ctgattttgt attattacta ggggatagag
     1981 gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca gcacatattc
     2041 acggaggcga agtgtctggt actgtagatg aatcaataag acatgccata acgaaaatag
     2101 catctgttca ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa atgggggaag
     2161 attcttggag agtttttccc gtcggagctc ctagaataga tactatttta aataatagat
     2221 tacctagttt tagagatata aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg
     2281 ttttgcaagt atttcatcca gttgtaaccg aactagaaaa tatagaaaaa caaattttag
     2341 ctttgatcaa ttcaatggaa caacaatctc aaccaattat ttgcatttta cctaatagtg
     2401 atgctggtag cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt
     2461 atattacgaa tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga
     2521 tcggaaattc ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt
     2581 taggaaccag acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg
     2641 aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga
     2701 aaaatgttaa gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga
     2761 atcaaatatt gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa
     2821 atatactagt gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga
     2881 aaccttaaga ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat
     2941 gcaagatctg aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa
     3001 aaaaatctat aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa
     3061 aaaattgaat atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc
     3121 aaaagaaatt tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga
     3181 tgctgtaatt ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag
     3241 aattaataac tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg
     3301 tgtaggaact caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg
     3361 gtgtatgata ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta
     3421 tggcaatcca gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat
     3481 caagtcctca tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt
     3541 ggatagcgcc attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg
     3601 gttctaaggg cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa
     3661 gactgcttaa tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta
     3721 cagtaaatcc tattttatat caaggggctg taccagtgtt cattgactca gaacctaata
     3781 catggaatat gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc
     3841 aattacctaa ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa
     3901 ttttagctat tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag
     3961 gttctactta taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta
     4021 acggtaataa gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat
     4081 atgttgagca ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac
     4141 atagcgaaat aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac
     4201 aaatggaagt attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg
     4261 aagctttttc tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa
     4321 atcgttggtt gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg
     4381 taataaataa actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt
     4441 tgcaaccagt atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag
     4501 aaaagttatt taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac
     4561 aagaatttgt tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca
     4621 taggaattat tggtttaggt tccatgggga aaagaagact tagactcatt caag
//
LOCUS       DACDBG010000132         4634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDBG010000132 DACDBG010000000
VERSION     DACDBG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.134"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IVZ90_002683"
     CDS             174..518
                     /locus_tag="IVZ90_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8263674.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IVZ90_002684"
     CDS             519..938
                     /locus_tag="IVZ90_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8263675.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IVZ90_002685"
     CDS             complement(1030..1380)
                     /locus_tag="IVZ90_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263676.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IVZ90_002686"
     CDS             complement(1346..2566)
                     /locus_tag="IVZ90_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8263677.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IVZ90_002687"
     CDS             2932..3498
                     /locus_tag="IVZ90_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8263678.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IVZ90_002688"
     tRNA            3643..3730
                     /locus_tag="IVZ90_002688"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="IVZ90_002689"
     CDS             4064..>4634
                     /locus_tag="IVZ90_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8263679.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DACDBG010000133         4628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDBG010000133 DACDBG010000000
VERSION     DACDBG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.135"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(179..790)
                     /locus_tag="IVZ90_002690"
     CDS             complement(179..790)
                     /locus_tag="IVZ90_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8263680.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(913..1038)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1120..1836)
                     /locus_tag="IVZ90_002691"
     CDS             complement(1120..1836)
                     /locus_tag="IVZ90_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8263681.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1842..2411)
                     /gene="scpB"
                     /locus_tag="IVZ90_002692"
     CDS             complement(1842..2411)
                     /gene="scpB"
                     /locus_tag="IVZ90_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8263682.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2395..3192)
                     /locus_tag="IVZ90_002693"
     CDS             complement(2395..3192)
                     /locus_tag="IVZ90_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8263683.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3162..3533)
                     /locus_tag="IVZ90_002694"
     CDS             complement(3162..3533)
                     /locus_tag="IVZ90_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8263684.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3552..4439)
                     /gene="xerD"
                     /locus_tag="IVZ90_002695"
     CDS             complement(3552..4439)
                     /gene="xerD"
                     /locus_tag="IVZ90_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8263685.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1277 a          998 c          800 g         1553 t
ORIGIN      
        1 aaacaaacat tttcgtcttt taaaaggaga agaatttcac atttgcaaag aattgattga
       61 tttttatgca taaatcaaaa aaatactcaa aacaaacaaa taaaacgttt acattattgc
      121 atgtcttgta tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt
      181 atttgactgc tgtatgacgt tgttcgaact gatgttgttt tcgacttaac catggaagaa
      241 gttgcgcatg cagcactaaa aagacagcac tcacgatgaa tcctttgatc aagttgaaag
      301 gaacgatccc aatcagtaca tagtttgcta aagacatgcc cagcatctgt gttgcgttca
      361 gccctaaaaa cattaagtaa agaggcgtaa taacgaaata atttgcgata ctcataaaaa
      421 tagttagagc gatggttcct gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt
      481 tctttacact aaagaaataa taaatcggga atgtaaagat cgttgttgat aagaaactag
      541 cagtgtctcc taccaaatta tccggagaaa aacctgtagt cagcagatgt aatactgaac
      601 gcaagaacgc tgttgcaact ccggccgctg gaccgaataa gaacatgctg atcaataccg
      661 agatatcact aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat
      721 actgtaaaac taatgcgatc gcagcaaaca tcgctactgc aaccattttc tgtacacggg
      781 tgttcttcat ttgaaatcct cctgttagga ctcctcttca gcgaactttt tgacgtttct
      841 ctccaaatga aaaaactcta ttttccaggg aaaatagagt tagagttatt tgatcaagcg
      901 tcgtcaaata agctctatct tctttatcca gactatactg tcggtatcgg aatttcaccg
      961 attcagctat ttttttgcaa aataggtcgt ggactataac caccggtcgg gaatttcacc
     1021 ctgcccctga agacgaacct tatataattt ttgtttacat attcattata catatctatt
     1081 caaaaaacgc aacatctaac aattataaaa agtaagtgtt cattttgagt ggttcagcaa
     1141 actgctgact tcttttttgt tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt
     1201 cagatctccg tatttttcac gttttagttt ttgcacagga agaccaactg cttgaagcat
     1261 atttttcact tgatgattac gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt
     1321 cttaggatcc actgataaaa tttggaattt agctggagat gtttttcgtc cttcgatctt
     1381 tattcctttt gtcaaaggtg caagcatcga ttttgtagcg atccctttta cctttgcaac
     1441 gtaaacttta tccacttcat gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt
     1501 tgtcagtagc aagatccccg aagtatcgta atccagtcga cctacaggat agatacgttc
     1561 ttttaccatc ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg caacagcaga
     1621 aataactcct ttaggtttgt acaataaata gtaaccgtat tcttcttgat aaattgggac
     1681 ttcatccact tccacagtgt catgtttccc aacttttatc cctaattccc ttacaacttg
     1741 tccgtttact tttacacgtc ctgcaaggat gaattcttct gcttttcgac gagaagtgat
     1801 gccagcgtgg gcaattactt tctgcaatct ctccattaca attattctcc tttattcatt
     1861 ccatcaaaga atagatctgg tgacacgtct tcttcaagtg aaatttccat ttcttgtgta
     1921 tcaggcagtt cttctaatga tttcaaacca aaataatcta agaagtagcc agttgttcca
     1981 tataatatcg gccgacctgg tccttctact ctaccttttt cttcaatcag tttatgggca
     2041 gctagacgct gtaccgctcc tgaaacctga actcctcgaa tctgctcgat ttcagctctt
     2101 gtgattggct gtttataagc gatgattgct aaggtttcga cggcggcttg agataatgtg
     2161 tttgatgtcc cttgcgcata tcgcttaagt aagggagcca gctctttttt agtcgttaat
     2221 acataatggc tttccgtttc aaaaatatgc aaagctgagt gcgagttttg agtgtaagat
     2281 tcttttaatt ctttcactaa acttgtgact tctgcttcgc tgagatttaa cagtccgctt
     2341 aattcttcat tactgatccc ttcctcgcca acaataaaaa gaagggcttc aagttcactt
     2401 aatttcgtca tgttcggtat ctcctactgc tggataaagc ataattgatt catagttttc
     2461 tttttgttgc gcaatgatct gccctttttt catcaattct agcaaagcca taaaagtagt
     2521 gacaacttca cttttagaag gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt
     2581 tctttttata cgctcttcga taaataccat tcgttcttcg atcgttgtgt catcgggcgt
     2641 gatcgttttt tccacttttt gagaaaattt cttacgttct aagattttct gcatggcacg
     2701 gatcaaatcg atttttttga cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc
     2761 atcgatgttc ataggttctt ttgtgtaata aagacttcgt tcctctgctt tttctgtcag
     2821 tctttcagct gcatatttat atttacggta ttctaaaagc tgagtgacca attcttctcg
     2881 aggatcttct tcccataatt cttcatcgtc tgtgccgaat tcttggacag gaagcaatgt
     2941 cttgcttttg attgccatca atgtagcagc catcactaag tattctcctg caacagctag
     3001 ctcgagtgtt ttcattgtat ggatataatt catatattgc tccgtgatcg tagcgatcgg
     3061 tatatcatat atatcgattt ccatttgctg aatcagatga agcagcagat cgagaggacc
     3121 ctcgaataca tctaatttaa tattgatctc cgtcaactca ttcactcttt cttctttttc
     3181 tttgctagcc attttccaat gtaaggctct gtgatttcag ttgcacggat ggagacatcc
     3241 gggtactttt cctgtaattc atctaacatt tgaaagccca tattctcacc gcgataagac
     3301 ggattcaaag aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta aataccaata
     3361 atcccttgaa tgttggttga accttctggc gtaaagaaaa aaagttcata atcgtcttca
     3421 gattcgtacg tatcaatctc cttcaacaaa gaagttttac ctgtcagttt cttatggaaa
     3481 ctcaatagtc ccatagcaat tttacggttt tctttccggt aatgtgttaa catcttctca
     3541 ccttcctatg actaagccct tggaaaatgc tgcttataaa catccgtcat tcttttcttc
     3601 gtgatatgtg tataaatctg agtcgtagag atatccgcgt gacctaacaa ttcctgtact
     3661 gtccgcaaat cagctccatt ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt
     3721 ggtgttactt ctttgtaaat cccagcctct cgtactaact gcttcagatt tttccagatt
     3781 ccttgccgag aaagaccttt cccatgatta ttgacaaata catgcatttc agatgcgtcc
     3841 tttacaagta atggacgtgc ttcgtctaag tagcgttcca gccattggat cgcataatca
     3901 cctaaaggaa tgatccgctc tttatctcct tttccaactg tctgtaccag tccaagagaa
     3961 agatgaagat ctcccaattt caatccaatc aattcactga cgcgcatccc agtagcatac
     4021 atgacttcca agattgcacg atctcttatc cctaacgttt ttgtcgtatc aggtgcttca
     4081 atcagctttt ctacttctgt cagagtcaat gtacttggta atttctgtgc tttcttcggc
     4141 gtatcgatat gttgcatcgg atcatgatca gtgatacgct cttgccgtaa aaactgatgg
     4201 aatcttctga gacttgagat catccgagta acagttgctg tcgctttttt ttcttcatgc
     4261 aacttctcca gaaaacggat gactaaaaag cgatcgacat cctgccatga agcaattttc
     4321 tgctcctgta gaaaatgcaa gtactgttcc aaatcacgtt catagctttg tcgtgtattg
     4381 gcggacaatc cccgttcaat attcaagtag tggagataat cgttgatttg ctctttcata
     4441 ataatccccc tcatgcctct ctatcatatc agataccgct tcattctgat agtctttttc
     4501 cataatctga gcaaagaaaa agccgtctag acactcagac ggactggttt cttttttcgg
     4561 ctctataatt tatgggactg atgaatcaga attgattctt cagccccatt ttcattttag
     4621 gtattaaa
//
LOCUS       DACDBG010000134         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDBG010000134 DACDBG010000000
VERSION     DACDBG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.137"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1142)
                     /locus_tag="IVZ90_002696"
     CDS             complement(45..1142)
                     /locus_tag="IVZ90_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8263686.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1250..3277)
                     /locus_tag="IVZ90_002697"
     CDS             complement(1250..3277)
                     /locus_tag="IVZ90_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8263687.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFFEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3422..4153)
                     /locus_tag="IVZ90_002698"
     CDS             complement(3422..4153)
                     /locus_tag="IVZ90_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8263688.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1262 a          923 c          716 g         1574 t
ORIGIN      
        1 aaccatccta caataataaa aatttactat ttgttatctt aaccttactt ttctttctct
       61 tctttttttc tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa
      121 gctactaaga tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat
      181 tccttggcta cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag
      241 ttgatcgtag tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa
      301 acagtcattg taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga
      361 aaagaaaaat ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact
      421 tcagaatgtc ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt
      481 tgatcatttt gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact
      541 tcatgaagag taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata
      601 atataggtgt attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt
      661 tccgtctgga tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc
      721 ggcatcctga catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt
      781 ggatagctca cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt
      841 tttccgtatt caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt
      901 ttcacttctt tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga
      961 tcaagttgaa gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc
     1021 gtcactgaaa aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa
     1081 ataactagga gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc
     1141 atatgtaaga gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac
     1201 ctgtgccctt cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg
     1261 ggacatctgc aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta
     1321 caggaatatc tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc
     1381 tttcaccctt ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat
     1441 tttcagaggt aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt
     1501 tctcttgttt caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc
     1561 cacctgaaaa ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat
     1621 tgacatattg aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat
     1681 cctctgtatt ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc
     1741 cttcaatatc aataggagtt tctttatact tctctcctat tttcccagtg attgttttca
     1801 tttcagcaat atcaatctga ctgtctttat ctttataata aacatggata tctatctcct
     1861 tcagctttgc gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa
     1921 ggtattcaaa atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca
     1981 attcgattat taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat
     2041 ttccggttat caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg
     2101 gaatagttaa ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaaaaaaat
     2161 ccccctcgac cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga
     2221 tggccccata atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa
     2281 gtcgcgtgac agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc
     2341 aatttacttc tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagtgac
     2401 cgacaccttc caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca
     2461 agcgccatgt gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag
     2521 aataattatt attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat
     2581 cagcagttat ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact
     2641 caatcgagag cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc
     2701 ccatacctgg cgatccaata acttgactgg gattactagc cattctagga tcgccagtca
     2761 atgtaaaggt catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt
     2821 taaatgtaaa atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag
     2881 acctttctgc tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac
     2941 cgataggtgt ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa
     3001 ccgcagaaat tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt
     3061 cagtggtctc tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg
     3121 attctattaa actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat
     3181 gctccaactg attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga
     3241 gactgcttaa aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt
     3301 aaattcctag atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg
     3361 ctcgattacc tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa
     3421 actagttgtt aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta
     3481 acttgacaga tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg
     3541 caccaaatcg atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg
     3601 tattgtttcc tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg
     3661 gatcaaccaa agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat
     3721 ccccactggc agttttaaac tgttctggtt gagagacact taatttccac ccagataagg
     3781 tacttctcct atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat
     3841 cagcagctgc ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt
     3901 ttgatccata atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat
     3961 cgacaggttt attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc
     4021 ctgcttcaaa tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag
     4081 aaacaaaagt cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt
     4141 cttttttcat cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt
     4201 aatccatagc aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt
     4261 tggtcctatg tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta
     4321 ttgcaacttg atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg
     4381 taagtccccc tcctatctat caaaatgtga cttgactgcc tagacttcaa tcaatattaa
     4441 ttataattaa ataaaaaatg tatattatat ttttt
//
LOCUS       DACDBG010000135         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDBG010000135 DACDBG010000000
VERSION     DACDBG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.138"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IVZ90_002699"
     CDS             690..1658
                     /locus_tag="IVZ90_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8263689.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IVZ90_002700"
     CDS             1669..2484
                     /locus_tag="IVZ90_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8263690.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IVZ90_002701"
     CDS             2552..3262
                     /locus_tag="IVZ90_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8263691.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IVZ90_002702"
     CDS             3298..>4425
                     /locus_tag="IVZ90_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8263692.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDBG010000136         4373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDBG010000136 DACDBG010000000
VERSION     DACDBG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.139"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            60..287
                     /locus_tag="IVZ90_002703"
     CDS             60..287
                     /locus_tag="IVZ90_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263693.1"
                     /translation="MKNEKSWHLAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILK
                     NENFFVSVLMGESLILILILCLIVHEVNKQL"
     gene            711..974
                     /locus_tag="IVZ90_002704"
     CDS             711..974
                     /locus_tag="IVZ90_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263694.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            1290..1720
                     /locus_tag="IVZ90_002705"
                     /pseudo
     CDS             1290..1720
                     /locus_tag="IVZ90_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            1859..2281
                     /locus_tag="IVZ90_002706"
     CDS             1859..2281
                     /locus_tag="IVZ90_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263695.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            2426..2671
                     /locus_tag="IVZ90_002707"
                     /pseudo
     CDS             2426..2671
                     /locus_tag="IVZ90_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2780..3736
                     /locus_tag="IVZ90_002708"
     CDS             2780..3736
                     /locus_tag="IVZ90_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263696.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
BASE COUNT         1471 a          654 c          891 g         1357 t
ORIGIN      
        1 tgaaaaataa tcctcctaca aaaattaatt ctttttatgg ctttagaact aaaaagtcaa
       61 tgaaaaatga aaaaagttgg catttagcac aaaaattgtt gggggatttt tcaaaaaaaa
      121 aagttatccc agtatttttg atgggtattg tttttttcag tatagagatt tatagtttag
      181 ctattttaaa aaatgaaaat tttttcgtta gtgtattaat gggtgaaagt ttaatcctaa
      241 tactgatttt gtgtttaatt gttcatgaag tgaataaaca attgtgaatt aaaaaaataa
      301 aaacctagta taatcacttt aataaattgt tggtagaagg tgatcaaaat gggagtaaac
      361 gaaatgatga aagtcagatt gagtttttaa atgatacctt cctcagaact tcaaaacgaa
      421 ttcaaagatt cgttgacaaa tcttgagcaa gatcatttaa aaaaatattt tctcttcatt
      481 tgacgaaaaa tgtttagaaa tcttatataa gtgaagataa agtaattggt gtaattaatt
      541 gtagagaata gcctttgagc aattgaagcg atacaattat ttagaaaatg aacttcctcc
      601 agcatactac atgattcctt acgggacgat gttaaagaag aaagcaagaa attttagaag
      661 tattagtaga taaactaatg ggcttttctc aatttaaggg agtagcccaa atgtttatca
      721 aatatggtgc agatacagta gcaaccatgt ttacagaaga atatcaagta gactattatg
      781 aagtaataga taaatgtgta cattttgttt atgaaaaagt gtggaatttt atgaattttg
      841 cgataagtga agaaatctta cgagtacaag aaaacagaac aggaatattt accagagaca
      901 gaaagcttaa tttgattgcg cattatatac ggaatcgctg gtcatcaata ccaaatttta
      961 attataaaaa ataattatga acaatctttt atacaatata cacaataaat caagtagtat
     1021 ttatgaaaaa agacttcaaa aacagaatga ttttctatca gtcttagaaa ttatagagct
     1081 aaaaattttg gtcacgcgta gaaacgcgtt ttttttgttt ttttgatagt aaagaacgta
     1141 caaaacaatg agaatttaaa agattgtact accagttttt ttaccttttc atttgagttt
     1201 agagtaagta aaatattact ataaaaacac atattcttga agaaatcttt tttttgctgt
     1261 attcatttag attatgatag agaaaatacg tgagaggggg acctgggatg tggctatctg
     1321 atgaaaaaat tcagtggcgt agctatcaga agcgtatatt gactaacttt gctacttatt
     1381 taaataacaa ttcagtccat ctagtggctc caccaggggc tggcaagacc tttttaggaa
     1441 ttgagatgat ccgacgtttg gattgtaaaa ccttaatttt ggttccttcc ctaatattga
     1501 agaaacagtg gttaacaaca atcgaggaat atttcttagt aaatccagca gatagttgct
     1561 atctttctgg ggcgattgaa catcctagtc atattacagt ggaaacgtat cagaccattt
     1621 acaaccgact gaaggaggag ccaacttatg gtgatcagct ggagctgtta gttttttgat
     1681 gaagctcatc atttaaaaaa aagctggggt gaattgttga ttgacttgaa gaaaaaacaa
     1741 ccgaatctgt tgacgatttc cttgacagcc acgcctccga ttgatagctc tcagacggag
     1801 tggaagcaat atattgagtt aaaatggcct gattgacgaa gaaattagca ttcccgagtt
     1861 ggtaaaggag gaggttctat gtcctcatca ggattttcta tatttgattc cggctaatca
     1921 ggaaactact cagcattatt ttaccttttt agagaaacaa aatgagctgg tggaagcgtt
     1981 aatggaaagc caagagacgg tggattattt gctttcttta gaatttgttc agcatccgct
     2041 tgagcattcg aaggaaattt atgaaaattt tgagctgttc actagtgcat taatttactt
     2101 gaatcatcat aactatgaat tgatacccga acattggcag ctattaggca gttctaagca
     2161 gcaaggtaaa aaagcttcag ttccatcttt aggtaaaaaa gagctgatca ctatttatca
     2221 gcagatgttt gaagatcaac cggaattgcc tattttttat caattgaaaa aagctggatg
     2281 attgtttgat gagtcgctgc atttgtttcc agagtatcca gacactgttg gtagtcaggc
     2341 gattcctgag aaaaagcaag cagtttgtca aattttgtta aaagaagaag ttcagctgaa
     2401 gcaagaactg acagcggtca ttttgatgga tcgtatttgc aaggaggtat tagcaggcag
     2461 tgatggtacc ttagcttatg gcgtggttcc tttgtttgat gaagttgttc gtctattagg
     2521 taacactcag caaatcggtg ccatctgtgg agagttctta ctgttaccga aaatgctagt
     2581 aactgttgag ctggcagaac tagcgacggg tatgaatgaa catccttata tgtcgggtta
     2641 tttttatttg acggtggatg acaaaaaccg ccaagcattg atgcaagcag ttactcgcct
     2701 attaaccgaa aagaagttga atattttggt aggaaccgtt agtttgctag gagaaggctg
     2761 ggattgtcct gcaattaatc tgttgatctt ggctaatcaa gcttccactt ttgtgcaaac
     2821 acaacaacta cgagggcggg gcttacgcct aggtgcacaa aataaagtaa cgaacatttg
     2881 gcatttagcg attggattac cgggagttcc tctgtctgaa cagcctgatt tgaaaagaat
     2941 gctacggcgt ttgggcaata tttcaggtct gacctttgaa tcttcaccaa aaattgagag
     3001 tggtggcgat cgctttgctt taccactgga atatacgacc accacgatgg aaacttatac
     3061 tcagtcgatg ttctattttt cttgtgaacg aagtcggttg aaagaacaat ggagtactgc
     3121 tttagctgat ggctggcacc tatctatgcc aatcgtcgtt aggaaagtcg atcaggtaaa
     3181 gcaaaaacaa gaaacttcat tgccgatagt agtaaaaaat atagagcctc taagcttctg
     3241 gtcagctata tttaaaggta acttgctgga atatttccaa attcgtaggc gacaaaagct
     3301 atgggaaaag gaatgtgccc gcaaaaaaag cttggccata tgcgttcaaa cgacgttgca
     3361 gcgccttggt gaagttagtg atagaaaagt aatcgtagcg gctgatcagc aggaatttag
     3421 atgctatctt gagaatgctg gctatcgaga aggacagctt tttactgaag ctatgaggga
     3481 gcttttagca ccgctggaga atccgcggta tttaatcgaa gggaaaaaga tagttctgac
     3541 ggtaccagaa cgctttggcc gaaataagca aatcgctaac ctttttctaa acgaattaaa
     3601 tcaatcgtcg ctgcccaaat gtcagttggt gtatactaga aatcctgaag gacgccaaaa
     3661 gctagtgact tatcgaatga ggcaagtctt ccagacccgc gatgaagtaa ttattcaaaa
     3721 aaacatttgg acgtaagttg atgaaaataa attttgctag agtagaacaa gtccgagaat
     3781 taagttgcta cttaactgat aatgccaagg acaggagaat cttttcttga cgactgaaag
     3841 aaactatttg acttatataa cggtgtgtga tgaatatttg caaggcattc tcttaaaaag
     3901 tactcgtaat agtttttttg aaaacaaaac agtactttat taataggact gacagagcga
     3961 tataagaaca tctgcagaca gaggtgaatg cttttctcat gatcgatgaa acaatggtac
     4021 ttcggatatt cttgctttta gtttgaaccg ttgtgatttt ctttgattcc taaaagtagt
     4081 tttatagaat gaaaatcaag catacggaat gctcctttga ttgtgttgtg gtgattcaat
     4141 ctaatggagc ttctatatgt ttttttattt gcaaaaagaa ccgatagaat atttttatca
     4201 gtcttagaaa ttatagagcc aagaaatttc aagcacacat aaaaacgcgt gttttatgca
     4261 ttttatgagc aaaaataact aaagaccatt acgtaaaaca taggtaaatc agcattcaat
     4321 ctcaaattat gtgatcaaat gttgatatta tttgattata agttttcttt tgc
//
LOCUS       DACDBG010000137         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDBG010000137 DACDBG010000000
VERSION     DACDBG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.140"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IVZ90_002709"
     CDS             complement(387..1853)
                     /locus_tag="IVZ90_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8263697.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IVZ90_002710"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IVZ90_002710"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8263698.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACDBG010000138         4289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDBG010000138 DACDBG010000000
VERSION     DACDBG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.141"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..759
                     /locus_tag="IVZ90_002711"
     CDS             <1..759
                     /locus_tag="IVZ90_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HAP8263699.1"
                     /translation="LKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQ
                     LKKEVEQYQSEKAKLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQ
                     SVIKSLDDQLSKGILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTAL
                     LPANESTYIKEGMKVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTT
                     QSFPFRYGLTGELSLIVGEKTYWQQIVDVFLNKK"
     gene            792..1826
                     /gene="sppA"
                     /locus_tag="IVZ90_002712"
     CDS             792..1826
                     /gene="sppA"
                     /locus_tag="IVZ90_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8263700.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            1828..2349
                     /locus_tag="IVZ90_002713"
     CDS             1828..2349
                     /locus_tag="IVZ90_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8263701.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            2346..2732
                     /locus_tag="IVZ90_002714"
     CDS             2346..2732
                     /locus_tag="IVZ90_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8263702.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            3761..4159
                     /locus_tag="IVZ90_002715"
     CDS             3761..4159
                     /locus_tag="IVZ90_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263703.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            4165..>4289
                     /locus_tag="IVZ90_002716"
     CDS             4165..>4289
                     /locus_tag="IVZ90_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263704.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMV"
BASE COUNT         1593 a          569 c          785 g         1342 t
ORIGIN      
        1 ttaaaagatt tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat
       61 aatgtttctg atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa
      121 caaattgagc aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt
      181 aaaccaacta cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta
      241 gaacaagcaa taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag
      301 aaccagtcag ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct
      361 gtatctggaa cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga
      421 acagtgcttg cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt
      481 ttacctgcga atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat
      541 caaaaaggtg gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct
      601 actgccactg agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg
      661 tttccgtttc gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat
      721 tggcaacaaa ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag
      781 gaggagttta tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt
      841 tactatccat tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa
      901 ccctaacagg aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag
      961 aagaaggcgc atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata
     1021 caggtgaaag caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc
     1081 taaagaaaat tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg
     1141 ggggaggaat atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag
     1201 atattcctat ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg
     1261 ctggttcaga taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta
     1321 tttctggttt aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata
     1381 aaagtggtgc attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata
     1441 aagttataca ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag
     1501 gaagaaatat ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta
     1561 accaagcagt agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt
     1621 ccctaaaaaa agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa
     1681 ctggctttgc ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa
     1741 ctacatcaga tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac
     1801 caatgtatta ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct
     1861 aattatgtat ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt
     1921 attagcatca tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct
     1981 gacaaatcca ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt
     2041 gtacttttga ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga
     2101 gtagtgagct ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata
     2161 tgtaggttta tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg
     2221 cccaagaaac aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca
     2281 ttagaagcta ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga
     2341 attaaatgaa taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga
     2401 ttttatgtag tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta
     2461 ctttaaatac tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga
     2521 atgttgtttt atttggagga attaccctag ttttttcgat aatgattttc ataataggtt
     2581 ttttagtaag aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta
     2641 ttattccatt ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg
     2701 cgggattgat gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg
     2761 attaatcaat taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac
     2821 atgtagataa aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact
     2881 ctctgattgt tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta
     2941 aatattcttc actttttaac cttttatagt acttagattt aatcctatat gtttagagag
     3001 ttcattggca gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact
     3061 gttcataatt atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt
     3121 aaaaataaaa agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga
     3181 aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg
     3241 gctcgaaccc gcgacccgct gattaagagt cattaatgtc aatttttaaa taaaattaat
     3301 atgtctcttt aagtattaaa ctatcactct ttatcctatg ataaatcaat gttttttaat
     3361 attagtagtg atatattaac atcaaataat attaactaag aaagataagg aaagatagaa
     3421 atagggaaaa aaatcggaaa aaatgttggc aaaaattttg ataaatttac tataaaaata
     3481 acgtcgatgc tttaataagt atcgacgctt tttacattat aggaaatata ttatgaatgg
     3541 tgtttaaaaa ttaatagaat caaacttttg ggcacatttc acttgatctt tttctcttta
     3601 aaatgaataa atgaaggcga agattctaca atctaaatat tagtaagtgt ttagttttta
     3661 cccgaaacgt attttagaaa ttaaatatca acaagaagaa actctgtgta attcgattta
     3721 tacagagctt tttttgcgtg tttttctagg aggaaaaaat atggtaaaaa acaaatttcg
     3781 cgcaacagat gtgctgacac aagcaacttt tcatatgcca aaactattct ttataaaatc
     3841 gtcaaaatat tatgcgatga ctgcacaagc aaaatacgcc tatagcttac tgaatgagta
     3901 cttagaagaa tccttaattg aaaaacggat tgatgaaaag aaacatgtct atatttctta
     3961 taccaatcaa gaattagcca ttttactcaa tgtgtctgaa gaagaaattg aagaaatcaa
     4021 aaatgaatta gaagattacg acttactaac aattgaattc ggtcgaattt atctaaaaga
     4081 attgattgct acaatggaag atgttcgtgt ctaccaacac caaaattctt tcttaatata
     4141 tggacaagat gaggtgtaat aacgatggca acaaaaagat ttaccgcaac tcgtaaatac
     4201 aatgaattat actatcaatt tccaaaggta tttattacaa gtgaaaaata tcgctcattg
     4261 tctgataaag caaaaatcgc gtatatggt
//
LOCUS       DACDBG010000139         4232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDBG010000139 DACDBG010000000
VERSION     DACDBG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.142"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(84..848)
                     /locus_tag="IVZ90_002717"
     CDS             complement(84..848)
                     /locus_tag="IVZ90_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8263705.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(876..1574)
                     /locus_tag="IVZ90_002718"
     CDS             complement(876..1574)
                     /locus_tag="IVZ90_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8263706.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(1586..2365)
                     /locus_tag="IVZ90_002719"
     CDS             complement(1586..2365)
                     /locus_tag="IVZ90_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8263707.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(2381..3325)
                     /locus_tag="IVZ90_002720"
     CDS             complement(2381..3325)
                     /locus_tag="IVZ90_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8263708.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(3371..4150)
                     /locus_tag="IVZ90_002721"
     CDS             complement(3371..4150)
                     /locus_tag="IVZ90_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263709.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1052 a          899 c          783 g         1498 t
ORIGIN      
        1 caatatgttt agaccaacca cttgagtttt ttctatacat ctctcactct ctcctcattt
       61 cctttttatc tacgaataaa gaactaaaat aaaccaaact tcttcttttt tacctcttca
      121 tagattggat aattgatctc atccccgttg atcagatcct ttgcgacttg tttcatctgt
      181 actacttttt ccttaccaaa ctccttggca atctgctcat agcactcttt taagtaaaag
      241 gtgcgcttag agaccccgtg tgcatcagaa gcagccatct gcacgagatt atgtttgacc
      301 attgttttgg ctgttttctg gatatctttg ccaaatacac caacataact tggcgcagta
      361 agttgggcca aacatcccat atctaggaac ggaagcaaat gatttgggtc ttctcgaaaa
      421 tgcgcgttgc gttctggatg gacgatcact ggtatctttc ctagttctaa caactcaaaa
      481 aaaagccgtt ctgcatataa cggcacttcc agtgtcggaa attcgatcag aagatatgta
      541 tcatctaaat ctgtaaataa aatttcatct cggtggatgt cttcgatgag cgttccggta
      601 atacgtactt cttgtccttc caataacgtc aaaggcaact gtcttttctc taattcggct
      661 tgtaacgaag cgactagaga aatgacttgt gatttttcat tgctgtattt tccattattg
      721 tggtgtggtg tgcataaaat atgcgtaatg ccttgctgaa tcgctttttc agccattgca
      781 atacttgctt ctaagttttc tgccccgtca tcgattcctg gtaggatatg gcaatgtaga
      841 tcgatcattt ctcttgtctc ctctcttctt attccttaat ttccgtagta ataaccagca
      901 tctttgctgt gttctgctcc gttgtatacg acacctaaga ctcgtgcttg taccatatct
      961 aacagttctt ttgctttagt caatgattct tttcgtgacg tattttcccg tacgaccaat
     1021 aaggtgccat ccgtttttga agacatgatc tgtgcatctg ttacggcaac gactggcggc
     1081 atgtcgaaga tcactacatc aaatagttgt cttgcttctg ctaaaatctg atccattctt
     1141 ggcgaactta gtagttctga tggatttggt ggtttaggtc cacttggcag aatcgacaaa
     1201 ttgtcgactg gtgttcgttg gatctcatct gctacacttc ctgatgaact aagaacagta
     1261 cttaatccgc ttgcgttgtt caacttgaac gttttataga tgacgggctt acgtaagtct
     1321 gcatcgacta gcagcacccg ttgaccagat ttggcaaaaa caacggcaat gtttgctgca
     1381 gtggtcgatt tcccttctcc aggtcctgat gaagtcacaa caatcgtttt gatttgctga
     1441 ccagctgcgg aggcaaactg gatattcgtc cgaatcgtac gatattgttc agcgattggt
     1501 gaagagggat tggtgagtgt gaccaaactg actgcatgag tttgggctgc tttttgtttc
     1561 tgtgttcgtg ccattttctt tcctcctaga ctcttgagcg gcttctgcgt gatggcgctg
     1621 ttttatcgtt tgtagctttt ggttgtgtct gagctggtcc ttttttacgg ataccagcat
     1681 tcaattcttt cgctgacatt tctggaactg ttcctaagat tgggaatcct aatgtatctg
     1741 tcacatattt ttcatctttc actgtccgat cgagtaattc caataagaat gccagaccta
     1801 cacctaccat cattcctaac attaagccag tcattaaatt caatttatta tttggcgaaa
     1861 caggtgtcgt attggcaact gcacctgaaa tgatcgagat tttatcaaca ttcatcacat
     1921 cttttgcgtt ctcttggaaa acttgtgaag ttgtgttagc gatctgttga gcagccactg
     1981 cattcgtact tattgcctga atcgagaaca tttgtgaatt ttctgcctga ttaactgaga
     2041 tggcatcttt gatttgttca gcagtcattt tcacattgtc ttctgtttct aaacgatctt
     2101 tgacttcatt gagcactaaa tcactgacga tcatatcttt atacgtattg atcatttgca
     2161 ggttcgtatt gacatcattg acatttgcag tgtctgattg tggcagtgtc acgatcaatt
     2221 gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt tacgattcct gagatggcca
     2281 agccagcaaa cattgagatg atgattatta ccaagtgttt gcgtaatgtt tcaaaaattt
     2341 ctttcaagct gattgtctct tccatttttt ctccactttc ctatttattc tctagattca
     2401 tttgatcttt taattctttt ttcacacgat caagttcttg atcactgact ttttgatagg
     2461 agatattatc ttgcatgaag ccttctcctt gcagctgatc ttgcttaatc gtaccaaatg
     2521 cctcccgata atctaaagca atttttttca tttcatcaaa gttcatgtct gttttcatat
     2581 tcgactctag agcattcaaa acggattgat agttgctgac accgtttaga ctcaatactt
     2641 ttttcgctac gccttctacg atctttcttt ggcgcccttg tctaccgtaa tcaccatttg
     2701 ggtcttcata acgcatacga gaataagata atgcctgatc cccgttcaat gtaattttac
     2761 cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc attattgact tcgataccac
     2821 caactgcatc aacgagttcc tttagccctt tcatattgat agagacatag tgatcgattg
     2881 ggatgtctac atatttttgt acggtatcca tcgccatggc agctccgccg aatgcgtaag
     2941 cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc gacatacgta tcacgaggaa
     3001 tgctgacgat cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc atcgtatctg
     3061 atcttccttg atcgacccga cctaaatctc cagtatcaat ccccatcaat agaacagaga
     3121 aagattcttg tttgtccaga tcaactggtt tcgttcgttc tccttgggtt cgttccactg
     3181 attcgtaagt tttctcaata gaatgtgaga gatccagata taattttgct cctactccta
     3241 caattgctaa tactaatacg ataagaactg ctaaaataat cagaaatatt tttttccctc
     3301 ttgattttct tcttcgtctt gccatagttt cctccgtttg ctaaccttgc ttgaatcaac
     3361 gttttaactg tcaatagctt atattgctat agcccatttg ctcagcaaca gtttgtgctt
     3421 ttgttgcgta tgctgcgctt attttttcat tccggatctg ctctatttga ctgagcaccg
     3481 tcaaatattg atcgtaagta gcggtggcag tcacttgacc atcctttaac atcgtatcga
     3541 ataactgatc tagttgcgtg acttgcgcga tctgaatatc agcgaatcct agatattgca
     3601 taacaaccgt tttccaagga ctatctttaa aaaagctcat attttcacgt acacgagcga
     3661 catctgcact tgtcaccttt tctttgatga tgacatcgtc tacagcttgc tgccagtttg
     3721 atacattttt agtaaataaa tcgttgacag ctgtctggat cttcagtttt gattctgcac
     3781 tatctaactg tgtcacgatt gctttcttct catcttggat tgcaacagct tctttaggca
     3841 attctgattc tttgatgtcg taatcatctg ctgaaagttt gacagaggat agcttatttg
     3901 acaaacttgt gattgtttct tctgttattt cagggtttag aaaaattttt tcttttgtac
     3961 tatacagctt gtctacttct tttgataaag aagtcaaggt gtctttttga ttggtgatgg
     4021 cagattctgc ataattgatc cgttgctgat gttgccgctg attattgata tggtatccaa
     4081 acaaacagat cgcaatagca cctgcaccta ccgccacagc agtcaatgac ttgacgaatg
     4141 gcttttccat agattcactc ctaagtattg atgatagata aattaagata taattcactt
     4201 acataaaaat gacagctttt tatttaaaaa aa
//
LOCUS       DACDBG010000140         4225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDBG010000140 DACDBG010000000
VERSION     DACDBG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.143"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IVZ90_002722"
     CDS             <1..572
                     /locus_tag="IVZ90_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8263710.1"
                     /translation="SKNQTSILELLDGYNSDLISEYKNEIETCVNEIRKLNWDIVSVK
                     KEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKN
                     LTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNK
                     SDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            575..1471
                     /locus_tag="IVZ90_002723"
     CDS             575..1471
                     /locus_tag="IVZ90_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8263711.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1521..1910
                     /locus_tag="IVZ90_002724"
     CDS             1521..1910
                     /locus_tag="IVZ90_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263712.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1897..>4225
                     /locus_tag="IVZ90_002725"
     CDS             1897..>4225
                     /locus_tag="IVZ90_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263713.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1451 a          653 c          884 g         1237 t
ORIGIN      
        1 tttcaaaaaa tcaaactagt atactagaat tactagatgg atataactct gatttgattt
       61 cagagtataa aaatgagatt gaaacatgtg taaacgaaat aagaaaatta aattgggata
      121 ttgtttctgt gaaaaaagaa atacaagata aggcgaaggt agagcttgaa attgaagatt
      181 taaaaaaaca agtagaaaag ctatctcata agagttataa agatgtttat gatgagtatt
      241 caaaagagac agatatttat agggaactga aacgtgatgt ggaggcactt aaggaaattc
      301 ccaaaaatct aactgatagt gttgacaaaa ttgactttgg caatggtata aaagttacag
      361 acgaaattga tgaacaccgc ggagaattga ttaagaattt agtgaccaat cgggctaaga
      421 ttcaaacaat aattaatgaa atgtcagatg aaattgatag ttatagagca gagctgaata
      481 aatcagattg gaaagttaac tataatgagg tttcaaagag gtatcaaaga ggtatcaaaa
      541 acttagtgaa gagttatctc aagatgaatt aaaaatgtta caaaaccttg attctttata
      601 tcaatcttta tcaaaaactg aagagcgatt ggaagaaatt attcaacaag aaataaagct
      661 taaagattac caagttgagg ttgactcagc aatgtcaaga atgaataaat catatcaaaa
      721 tctctattct gagagaagta gttcctcgcg cgaaattttt tctgatatta caggaataga
      781 ggctactatt caaccaaata gggatttcaa taattatgtt aaagagctca gagcttgtat
      841 taataaggag gacacttttg acagtgagtt tggatctctt attgataagc tgacaaaaaa
      901 ttccattact aatcaagata tatataatga catacttgat ataagagtta gtggggaaag
      961 tagtattttt tcagataaaa aattaatcaa ttctataaga aaattgaatg atgcacaagt
     1021 tcttgaatta caaaccctta agccaaatga tttagtagtt atccgacttg aactaaatgg
     1081 aaaaaaagtt agtttatcga atgctagtgc tggtcaaaag acatctgcaa ttctttctat
     1141 gattttggcg tatggaaatt caccactcat attggatcag cctgaagatg atttggatag
     1201 tcaattaatt aataacttaa tcgtaagaag tatcattaca aaaaaggaaa acagacaaat
     1261 tttaattgta actcataatg caaatatccc agttaatggt gattcagaat ggttgattag
     1321 catgggagat tcaaaagaaa tatcggctga tcagtgcggg tcggtagatg aacccaatat
     1381 caagccaaga atatgtactg tgatggaagg tggcgaggat gcttttacaa atagagcgaa
     1441 acgctatggt tttaaacaaa ttgtagaata acactagagg gtgagacata ttattatgtc
     1501 cgcccttttt aggaggaaaa atgaaaaaaa taaaaagcta taccagtatc tgggcagttg
     1561 aaaaagtcat ttacgcaatc aatgattttc agttgccttt tcctgtgact tttaaccaaa
     1621 tggcttggtt cgttttatct ttactgtttg tcatcgtatt tgcccatgtg cctccactat
     1681 ctatgataga ggaggtcttt ttaaaatact taggcatacc ggtggctgtc acttggttta
     1741 tgtcgcaaaa gacgtttgat ggcaagaaac cactgggatt tttacgatcc tttatcagct
     1801 atcacttgcg atttaaggtg acttttgcag gaaagaaagt caaagaacag aagaaacgat
     1861 gggatgaacc cagtactctt gtaaggagtg tgaactatgt atccgattaa gtatattgaa
     1921 aataatctcg tctttaacca agaaggcgag tgttttgcct actatgaatt agtgccttac
     1981 aattactcct ttttgtcacc cgaacagaaa tttcaagtgc atgataactt tcgccaacta
     2041 attgcgcaaa atcgtgaagg caagatccat gcgttacaaa ttgccacaga aagtagtatt
     2101 cgcgccacac aagaacgatc aaaaaaagaa attaccggta gactcaaaga agtagcgaaa
     2161 caacgaattg atatacagac agatgcatta gtatccatga ttggcgatag tcagattgat
     2221 tatcgctttt ttattggctt taaactaatt gcgacagatg aagaagtcaa tctaaagagt
     2281 ctcaagaaat ctttcttttc tgggcttcaa gaatttgtct atggagtgaa tcatcatttg
     2341 atgggcgatt ttgtttcctt atccaatgaa gaaattcgct gctattctaa gcttgaaaaa
     2401 ttaatggaaa gcaaattggc tcgaagattt aaagtcaggc gagtcacacc gagtgacctg
     2461 acgtatttga ttgaacatat ttatggcgaa aaagggatac cgtttgaaga atacgaattt
     2521 cagctaccaa agaaaaagct aaaatcagaa acactggtga aacgttatga cttattacgt
     2581 ccaaatcgtt gtttaattga agaaaaacca aggtgcttac ggatggaaca tgagaatcat
     2641 gagtcgtacg tagcgtattt aacaattaat acaattgtgg gggagatgga atttccttcc
     2701 tcagaacttt tctattacca gcaacaacaa tttacattcc caatcgacac aagtatgaat
     2761 gtagagattg tgacgaataa aaaggcttta gctaccgttc gtaataagaa aaaagaactg
     2821 aaagatttag acaatcatgc ttaccaatct gacaatgaaa cgaattccaa tgtcttagat
     2881 gctttggatt ccgtggatga attggaaacc accttggatc agtcaaaaga atctatgtac
     2941 aaactgagtt atgtggttcg agtgagtgcc gaatcagtgg atgaactaaa gcgacgctgt
     3001 gatgaagtgc tcgattttta tgatgatacc aatgtaaaac tagtccggcc ctttggcgat
     3061 atgatggggc tacatgaaga attcttaccc tcaagtaaac gatatatgaa cgactatatt
     3121 cagtatgtta cgtctgattt tttggcgggg cttggttttg gggctactca gatgctagga
     3181 gaattagaag ggatttactt tggctacaat gtggatacag gtcgtaatgt ttacctgaaa
     3241 ccggcattgg cttcacaagg agtcaagggt tcagtaacca atgcgctggc cgccgctttc
     3301 ctgggttctc ttggtggagg taaatccttt agtaacaatc tcttagtcta ctatgctgtt
     3361 ttgttcggtg gtcaagctgt gattgtcgat ccaaaagggg agcgaggagg gtggaaagaa
     3421 accttgccag aaattgcgga tgaaatcagt atcctcaact tgacgagtaa gtcagaaaat
     3481 caaggtctgc ttgatcctta tgtgattatg aagaaaaaga aagactcgga aagtttagca
     3541 attgatattc tgactttttt aacaggaatt tcaagtcgag acggggagaa gtttcctgtg
     3601 ttacgtaaag ccattcgaac ggtgactcaa agtgatcaac gggggttatt acaagttatt
     3661 aacgaattgc gaaaagacgg gtcaccaatc gcggaaaata tcgccgatca tatcgagtcc
     3721 tttaccgatt atgattttgc tcatttgctt ttctcagacg gctcgattac ccaatcgatt
     3781 agtttagata aacagctaaa tatcattcaa gtggcggatt tggttttacc ggatgcagaa
     3841 acgacttttg aagaatatac caccatggaa ttgcttagtg tttcgatgct aatcgtcatt
     3901 tccacttttg ccttggactt tatccatagt aaccgagaaa tcttcaaaat tgtcgatttg
     3961 gatgaggctt ggagttttct tcaagtggct caaggaaaaa cgttatcgaa taagcttgtt
     4021 cgtgctggtc gggccatgaa tgctggggtc tatttcgtca ctcaaaatag cgacgattta
     4081 ttagatgaac gtttaaaaaa taatatcggg ttgaagtttg cttttcgcag taccgatatt
     4141 catgaaatca agaaaacctt ggaattcttt ggtttggatc aggaggatca aagcaatcaa
     4201 aaacgcctgc gagatttgga aaacg
//
LOCUS       DACDBG010000141         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDBG010000141 DACDBG010000000
VERSION     DACDBG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.144"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IVZ90_002726"
     CDS             <1..106
                     /locus_tag="IVZ90_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8263714.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IVZ90_002727"
     CDS             970..1182
                     /locus_tag="IVZ90_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263715.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IVZ90_002728"
     CDS             complement(1460..3259)
                     /locus_tag="IVZ90_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8263716.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IVZ90_002729"
     CDS             complement(3383..3979)
                     /locus_tag="IVZ90_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8263717.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDBG010000142         4095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDBG010000142 DACDBG010000000
VERSION     DACDBG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.145"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IVZ90_002730"
     CDS             complement(147..773)
                     /locus_tag="IVZ90_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263718.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IVZ90_002731"
     CDS             complement(1068..1403)
                     /locus_tag="IVZ90_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8263719.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IVZ90_002732"
     CDS             complement(1390..1674)
                     /locus_tag="IVZ90_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8263720.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IVZ90_002733"
     CDS             complement(1730..3253)
                     /locus_tag="IVZ90_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8263721.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..>4095)
                     /locus_tag="IVZ90_002734"
     CDS             complement(3246..>4095)
                     /locus_tag="IVZ90_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8263722.1"
                     /translation="NGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILH
                     FKYFTQDGLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAK
                     NAIREKFEEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAF
                     GVPIERLGVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETD
                     PETKVKNVLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQE
                     GVTPVE"
BASE COUNT         1179 a          854 c          609 g         1453 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttgaacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttg
//
LOCUS       DACDBG010000143         4094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDBG010000143 DACDBG010000000
VERSION     DACDBG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.146"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IVZ90_002735"
                     /pseudo
     CDS             1..492
                     /locus_tag="IVZ90_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IVZ90_002736"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IVZ90_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IVZ90_002737"
     CDS             1171..1617
                     /locus_tag="IVZ90_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263723.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IVZ90_002738"
     CDS             1619..1756
                     /locus_tag="IVZ90_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8263724.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="IVZ90_002739"
     CDS             1794..2087
                     /locus_tag="IVZ90_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263725.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="IVZ90_002740"
     CDS             2084..2308
                     /locus_tag="IVZ90_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8263726.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="IVZ90_002741"
     CDS             2305..3330
                     /locus_tag="IVZ90_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8263727.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="IVZ90_002742"
     tRNA            3520..3593
                     /locus_tag="IVZ90_002742"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1424 a          695 c          823 g         1152 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct tttt
//
LOCUS       DACDBG010000144         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDBG010000144 DACDBG010000000
VERSION     DACDBG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.147"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IVZ90_002743"
     CDS             complement(85..339)
                     /locus_tag="IVZ90_002743"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8263728.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IVZ90_002744"
     CDS             complement(450..704)
                     /locus_tag="IVZ90_002744"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8263729.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IVZ90_002745"
     CDS             complement(897..1172)
                     /locus_tag="IVZ90_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8263730.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IVZ90_002746"
     CDS             complement(1144..2097)
                     /locus_tag="IVZ90_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8263731.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..>4073
                     /locus_tag="IVZ90_002747"
     CDS             2709..>4073
                     /locus_tag="IVZ90_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8263732.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRL"
BASE COUNT         1343 a          698 c          676 g         1356 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga cta
//
LOCUS       DACDBG010000145         4071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDBG010000145 DACDBG010000000
VERSION     DACDBG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.148"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(180..1685)
                     /locus_tag="IVZ90_002748"
     CDS             complement(180..1685)
                     /locus_tag="IVZ90_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8263733.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1682..2368)
                     /locus_tag="IVZ90_002749"
     CDS             complement(1682..2368)
                     /locus_tag="IVZ90_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8263734.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2561..3982)
                     /gene="gndA"
                     /locus_tag="IVZ90_002750"
     CDS             complement(2561..3982)
                     /gene="gndA"
                     /locus_tag="IVZ90_002750"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8263735.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1120 a          859 c          694 g         1398 t
ORIGIN      
        1 attacagcat tttattgttt tttcgttcat ctaaaagata cttacattta attaacggat
       61 gttattctat ttaattgttc gctagttttt ttataatgac atccctgaat ttctcacttg
      121 cttgtatttt gtaccttatt ctttgttgga tatataaaaa aacaagtaaa gcacatgctt
      181 tacttgtctt ctgcctttgc gataggaata aagattctaa aaattgtccc ttgccctact
      241 acactttctg ctaagatacg tcctttatag ttttcaacca gttgtttagc gatagacaac
      301 cccagaccat ttccaccctt cgttctagct cttgctttat ccactcgata aaaacgattg
      361 aagatcttat taaggtcttc tttgggtatt ccttcgccaa agtcctgaat cgcgatctca
      421 aattcattca gagtggaaga aatagagata tggacttctt ttcgtgtagt tgaatacttt
      481 actgcgttat ccaatatgat aatgattaat tgttcaaagt gattacgata aatttgcagg
      541 gtcacttctt ttgtcaaatc atcatctaaa gtgatcacat agtctggata taatatcttg
      601 aaattattat aaacttgata ggtgacttct tttcctttag ttgtttcatt cgcataatga
      661 acatctactt gttctgctct tgataagtct agcatctctt gaactaagct tttcatacgg
      721 ctgatttctt gcatgctggc gcttaatgat tcttctaata tctcaggatc atctttcccc
      781 caacgattta gcagactaag atgcccttca ataattgcaa caggtgtcct taactcatga
      841 gatacgtctt ccacgaattg ctcttgttgt tcaatataca aacgcatacg gtcaagcatc
      901 tcattgaata tttcagctaa atctgccaat tcatcatttg tgtcgatttc aggcatgtgg
      961 atatctgatt gcggatcttt acgtatagta tccattgtat ctctgagtac cttgagtggt
     1021 tttaagaaat aagaagataa aaagaaacct aaaatactac ttaagatcaa agaaacaact
     1081 tctaaaacaa taagagtcaa taatagctta tttcgaatat catagaaaga agataattca
     1141 tagaagcttt gcgcatagcc gatcttttct cttgtacctt tagaatatat aggctggata
     1201 gataaaaagc cagtttttcc ttctagtgta acgatcgttg gaactttacg tgtagtctga
     1261 atcaggtttc gactgttttc atgggtttta aaaatcaagt tctcgtccaa atcataaaca
     1321 taaagatcca ttgatggctg ccctagctca gctataaagg aatcgatctt cattaagttt
     1381 ccttcgacca gtgaagtatg atctacgacc tcatctccgt catttgtcgc tttcgtcaga
     1441 ttacgatacg tatttactaa tgtcagttct tcatccgagt tagccaaacg agaagctact
     1501 tctgaaatcg tacgctctac attgtttctt tcttttgcca caatcagatt gatagatgat
     1561 ttataggtaa tgacagcgaa aattgtaaac acaacgaata tgaagaaaga actcgcaaag
     1621 gcccacttga ttgtcaagga gggccctttc agttctcttt tttccgattt tttcggcttt
     1681 atcacgagcg catcacataa cctgttccgc gtacagtttg gatatagctt tcctccccgg
     1741 gaacgtcaat tttgttgcga agataacgaa tatatacgtc cactacgttt gtttcaactt
     1801 ctgtttcgta tccccacact ttatttagta aaacgtcacg tgctaatacc acgttcacgt
     1861 tttccattaa agtaagcagc aactcgtatt ctctttttgt tagttcaatt acttcatttc
     1921 cgcgacgtac gacacggttt tctttttcga tagtcaaatc gcgataagta atagtagttt
     1981 gtttagccac atttttatcg ccttcaatat cgattcgacg caacaatgca cgaagacgtg
     2041 ctaataattc ttcgatagca aatggtttga cgatgtagtc atctgctccg tggtcaagtc
     2101 cagatacacg atcgatcaca gaatctcttg cagtcatcat aatgattggt gtatttttga
     2161 cttggcgtac acgtctgcag acttctaaac cgttcaattc tgggagcatc agatccaaca
     2221 atatggcatc ccactcatta tttaatgccg catctaaacc agttcggccg ttgtagtgga
     2281 cttctgcgtt atacccttca tgcttcaact ctagctcgac aaatcgtgcc aagttctttt
     2341 catcttcaat gattagaata ttgctcattt attattttat cctttctatt ctactggctt
     2401 tttaaaaact ctatcattat accgataata tgcttaaaaa tctatagtca ttttatcatg
     2461 gatagcaatt ttaaaaaaga gctgaagtct actcataaca atcgacttca gctctttcgt
     2521 cctcttttta ttttctcatt tcctaacagc cgaaagacta ttattcttca tggtaccatg
     2581 agtaatggaa gattccttct ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc
     2641 gttgggcttg gattatattt gcaggcagac gttctgctct gtatgaatcg aagtaagcga
     2701 tggctgatga gaaagtcggt acaggtacgc cagcttggac agccaaggca acgacgtctc
     2761 tgactgattg ctgatatttt ttcgtgattt caacgaagta atcatctagc aataaatttt
     2821 cgatctcagg atttttgtca taggcatccg taattttttg caagaatcga gctcggatga
     2881 tacatccagc gcgccagatt tttgcgattt caccaaatgg caagtcccaa tcaaattctt
     2941 ttgaagcgac acgtaattga gcaaatcctt gggcataact catgatttta ctgaaataca
     3001 acgcttctcg gattttttcg accaattctt ttttgtctcc cgtgaactca aaatcgttcg
     3061 tacgagacaa aatcttgctt gcttgtacgc gttcttcttt ataagcagaa atatagcggg
     3121 caaatacaga ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg
     3181 tccatttacc tgttccttta ttcccagctg catcaaggat cacatctact actggtttac
     3241 ctgttccttc atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt
     3301 ctaattcgcc ttgattccat tctttaaaga tatcagccat ttcttctact gaaaggttta
     3361 aaatattctt cattaagtca taagattcag caatcagttg catatctcca tactcaatac
     3421 cgttgtgtac cattttcaca taatgtccgg ctccatttgg gccaatatat gtgacacatg
     3481 gtgctccgtc ttctgctttt gcagaaattt gctctaagat tggtgcaact agcgcatagg
     3541 cttctttttg tccaccaggc atgattgaag gtccttttaa agctccttct tcaccaccgg
     3601 aaacgcctgt accgataaag ttgatgccag agttggctaa ctcttcatta cgacgcatcg
     3661 tatctttaaa gaatgtattt cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa
     3721 gtaactcttg gattgttgca tctgttgccg gtcctgcttt gaccattagc aagatccgac
     3781 gtggtttctc gatagattct acaaattctt cgattgtata tgttgctttg aaatttttat
     3841 ctgggtgttc ttctactaca gcggttgttt tagcccctgt acgattgaat aaagccactg
     3901 aatagccgcg gctttctatg ttcaaggcta gatttttacc cataacggcc ataccgacaa
     3961 cgccgatttg ttgttttgac atatgaactc ctcctacata cgggatttct ccctaatctc
     4021 ttacattata gcttaacacg gctacattgc aaactaaaga acaacatatt t
//
LOCUS       DACDBG010000146         3859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDBG010000146 DACDBG010000000
VERSION     DACDBG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.149"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1170
                     /locus_tag="IVZ90_002751"
     CDS             <1..1170
                     /locus_tag="IVZ90_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8263736.1"
                     /translation="IKGKLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILEL
                     KRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGT
                     VTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRR
                     LYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSF
                     NQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTE
                     IVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHC
                     YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1553..2281)
                     /locus_tag="IVZ90_002752"
     CDS             complement(1553..2281)
                     /locus_tag="IVZ90_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8263737.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2259..3833)
                     /locus_tag="IVZ90_002753"
     CDS             complement(2259..3833)
                     /locus_tag="IVZ90_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8263738.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
BASE COUNT         1196 a          673 c          696 g         1294 t
ORIGIN      
        1 attaaaggaa aattggatga ctctccttcc tattgtgaac actgtcgatc ggttcgtatt
       61 atccgcaacg gttcctatac gacacgcact cagatattga aagtcaaaga aaagttaact
      121 attttagaat taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg
      181 aaaacggact tagttgatga acatcatcaa ctaacaaagg aactgaagca ggcaattctc
      241 atggaactgt acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat
      301 gggacggtga cacggatctt gcgtgaagca acgaagcatt accagtcacg tatgaacttc
      361 cttccgacgg tgttgtgtat ggatgagttc aaatcaatga agtctgtttc tggttccatg
      421 agttttattt gtgttgatgg gacaacccat cagctattta cgatccttga agatcgcaga
      481 ctttacaaac taactcaata cttcctgcac tttcctagaa aagcccgttt aaaggtcaag
      541 tacctagtga tggacatgaa tgccagctat tgtcagttgc ttaaaacagt ctttccaaat
      601 gcggagatcg taactgaccg ctttcatatt gtccaacaca tcaatcgcag cttcaatcaa
      661 ttacgtgtcc agatcatgaa tcgttttcga acctcacact ctgaaaatca gaagaagtac
      721 cgtcggctca agcgatattg gaaactctta ctcaaagatt ccacgacatg ggagccactc
      781 aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat cgttgatgag
      841 ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt attacgctat
      901 tatttcgtta aacgaggtac ggaaggcttt caagaaaccc ttaaaacaat ttcatctacg
      961 ttacctcagt cgtttaaaaa gaaattcacg atcttccatt gctaccaatc gggtatttca
     1021 aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgaataa caagatcaag
     1081 ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc acggatctat
     1141 ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga acgcgccgca
     1201 tatcggacta agttttattc aaatttgtct tcaccaacac gatttgacga agagcctatt
     1261 taaataacta catccattta ttctatcttc taaaatagcc tttttcattt cagttaaata
     1321 gttcagcgcc atttttgcac tcgacttgca tagtagtttt ttccatattc ctcaacctca
     1381 gcgggggttg aagacataag gaaaatccta tgcgttatgc tcaactggat ctgattaaat
     1441 attctaatgt atttttctct accatcatta aatggttaat agccaaaaat caccactttt
     1501 caatggctcc ttgttgtata agtataacta gaagatgaac acgtctgaga aattatagaa
     1561 gctttttacg gtaattactg gggctgtgtc cagtagtttt tttgaagaga acagaaaaat
     1621 agctcacgtg atctattcct actttttgac taatttcatc aatagataag aatgtgtctt
     1681 tcagtagagc acttgctctt ttgattcgaa agtctagcaa gtaatcatga aaggaataac
     1741 ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg gtaatggaat tgtgtagcta
     1801 catctttgag cgtaagcttt tcactataat tgttttgaac gtaatcaatc acttgttgaa
     1861 ttgactcatt aaaagaatca tcagttagag agttttgatt agctgctgat ttacgcattt
     1921 tttcttcttg tactaactta actaaataag gaaaatcaat gggtttcgtt atgtaacttt
     1981 taatagataa atcaatcccc ttttttgcat actgaaaatt ttggtgggca ctaatgacaa
     2041 tactagtaaa gtagcgattg gatgtttgtt gcaaagattc gattaattcg agtccagaaa
     2101 ttttccccat cgtaatatct gtgataagta aatcaaagcg ctggttttgc aggtttttaa
     2161 atgcttcgtc tccgtctgtg tagcagtgat cgataatgat gtcggggact ttcttcaaaa
     2221 gataatgcct tatgccttct acaattgtac aatcatcgtc aacaattgca attctcaaca
     2281 tgcatattcc cccctttttg tattttgata gtaatcgttg ttccgacgtg tagttggctt
     2341 tctataacaa aacgtgtatc tccaggatag agatgcttta tacgtcgaaa agaatttgag
     2401 atgccgatat tctcactata aggaatatca tcattaagtt tttgttgaat atgctccagc
     2461 ttgtcttggt ccattccttt cccattttca acgattttta acacataatg gttggttgtt
     2521 tcagttgcat agacagtcaa acgattttcc ttttttgatg aatcgaagcc gtgtttaata
     2581 taattttcaa caaatggctg taaagaaagt tttggtaaca ttaattgatc aatatgatca
     2641 atcagctgat agtcaaagtt atgtcgataa cgacgttgat gtagttgcaa ggagaggtcg
     2701 atattaatta attcctcttt caaatcaatt atgtcgggct gatatgtatt attgcgatac
     2761 aacttgctca ataaatatat actgtcgctg atttcttcgt catcattcaa tatggcagtc
     2821 attcgaataa attccaaatt attaaataga aaatgaggct cgatttgata ttgaagtgct
     2881 gcatactttg cttcttgctt caaccaatta ttttcataaa gtgttttttg atatgtagca
     2941 ttatctaaat aaaactgatt gatatgcata gcgatttctt tcaaatcacc gactttgttt
     3001 tcgatattga ttgacttgta gtctcctgta gatgaatgat ttaaggaagt aatgatttcc
     3061 tgatactgct ggatatagtt acgaaaactt ctatttaata attgatgaat agcaagaata
     3121 aacaaactca ttaataagaa aatcatgatt gccagaccta ataaggtttg tgacgaataa
     3181 tttggatttt catatacttg taatgtgtgg ttaccgatag ctatctgttg cgtttttttc
     3241 catttagaag gcgagatgtc ttcagataaa ttgacggaat caataccatt agacaaagaa
     3301 taattgtaat gtgttggaaa gagactcttt tttagcgcgt tgatcgaaac gtctagaatc
     3361 aatgtagcat cgggcatgtc ctcactaatg aaacttaatg aacgaataaa agaaattttt
     3421 tcgttttttg taggtgcgta ggcacttaag tatttttcgt aatctaatga gatagatata
     3481 tttttaactt catcatcttg cacaagtttt tttaagttat ctactaaata ggtaggggag
     3541 cgatcctttg acaaaatatc ttccatgtat tcagcaggtg atgagaggta gtagtcatta
     3601 aggttttctt ggaaatcata attttgatag atctctgtca cgataccgga gagaatacga
     3661 ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt gcaggttttg atttttttct
     3721 ttttcaatca cattgatata ggaaaagaac aatgtgaaac aggcaagtaa agtaataaaa
     3781 aaaatcaata tgtatgtaaa acgcataata acttttgata aatcgttttt cattcaaagt
     3841 cctcattttg ataatctct
//
LOCUS       DACDBG010000147         3753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDBG010000147 DACDBG010000000
VERSION     DACDBG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.150"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..115)
                     /locus_tag="IVZ90_002754"
     CDS             complement(<1..115)
                     /locus_tag="IVZ90_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8263739.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="IVZ90_002755"
     CDS             246..698
                     /locus_tag="IVZ90_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263740.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="IVZ90_002756"
     CDS             712..1401
                     /locus_tag="IVZ90_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263741.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..>3753
                     /locus_tag="IVZ90_002757"
     CDS             1526..>3753
                     /locus_tag="IVZ90_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8263742.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DK"
BASE COUNT         1267 a          507 c          771 g         1208 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aat
//
LOCUS       DACDBG010000148         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDBG010000148 DACDBG010000000
VERSION     DACDBG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.151"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVZ90_002758"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVZ90_002758"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8263743.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IVZ90_002759"
     CDS             complement(2260..2511)
                     /locus_tag="IVZ90_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8263744.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IVZ90_002760"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IVZ90_002760"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8263745.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IVZ90_002761"
     CDS             3461..3679
                     /locus_tag="IVZ90_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263746.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACDBG010000149         3564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDBG010000149 DACDBG010000000
VERSION     DACDBG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.152"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(213..1565)
                     /locus_tag="IVZ90_002762"
     CDS             complement(213..1565)
                     /locus_tag="IVZ90_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8263747.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(1837..2283)
                     /locus_tag="IVZ90_002763"
     CDS             complement(1837..2283)
                     /locus_tag="IVZ90_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8263748.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(2519..3445)
                     /gene="cysK"
                     /locus_tag="IVZ90_002764"
     CDS             complement(2519..3445)
                     /gene="cysK"
                     /locus_tag="IVZ90_002764"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8263749.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         1000 a          754 c          566 g         1244 t
ORIGIN      
        1 ttctaaaaat gcatcttgtt ggacaaaata ttctttattc atcttctttc tcacctttct
       61 tttcaaaatc aaataaagct gcttgttctt ttatgtaaca gctttatctt tgctgcttat
      121 tgtgacaaaa aattcgaaga aagacaacta attgtacttt tcacaaaaaa agacagaggg
      181 tcgtgacaaa attgtgtcac aacccttttt ctttattgtg cagtttcgat ttcgcctagc
      241 gcttcttgtg caagtaggtt caagtaattc catgggcggt caaaatgtgg ttggaagaag
      301 aaatctgaaa gtgcgagatc ttctacagtc attttatttt gaacagctaa agacatggta
      361 tttgctgatt gtgtaatgtc gtatttggac atgaactgag cacctacgat acgattcgtt
      421 cctttttcat agactagctc catcattact ttttcagtag ttggcatgaa ctctggacga
      481 taattatctt caaatacagt cgcttggact tctagatcgt tcaaagttgc gctttctttg
      541 gtcacacctg tcgaaccgat cgtccagcca aataggtaaa gtcctgaagt tccttgagta
      601 ccacgatacg ccattttatg atctgtcaag ttatttccca caagtaaacc ttgacgaacg
      661 gcatttgttg ccaaaggaat atagttttgc gtgccacttg gattgtagtt gactactgca
      721 gagtctccag ctgcaaaaat gtctggatca cttgtctgca tgtattcatt cactttgatt
      781 gccccgtttg gcagtgtatc tacttttcct tcgatcagtt ttgtatttgg acggaagccc
      841 acacataaaa tgaccatatc tgtatcaaat gtatctgttg gtgtagctac ttgtttcact
      901 tttccttctt catctgtgat aaattcagta acattttcgc ctagcgcaag agtaacaccg
      961 cggtctgtca attctttttc taagatatct gtgaatggtt tatctagata tttattcaag
     1021 atgcgatcta atccatcaat caatgtcact tgttttccag attcgacaaa agcttctact
     1081 aattcaatac cgatatatcc accaccaaca attactacac gttttgcttc tttcgcttgt
     1141 gcaataattt catttgcttg attgtagttt ttgcatagga gtacattttt cgcatcgatt
     1201 ccactaatcg gtgggataat aggccatgaa ccagttgtca tcactaattt atcataagaa
     1261 accgtttctg tttctcctgt tttcagatct tttgccgtta ctgttttatt ttctgtatcg
     1321 acgtctgtta catcgtgttc cattttaact gttgctccta atgaagataa ttcttcagga
     1381 tttgagtaga acaagcctgc tgggtccttt actactccgc cgacataaag tgcgataccg
     1441 caagataaga aagaaatatt gtcatttctt tcatatactg tcacttctgc ttctggatga
     1501 ttttttaaaa tactttttac agctgcagta cctgcatgag tacagccaat tactactact
     1561 ttcatttatg gtccctccga ttaacatttt gtccttcgac aaaactacta taacagaaat
     1621 aaaacggata caacagttta atgcttgtga attcacaaag gtttgtttgt agtttcattc
     1681 cttttcaagc ataagaaccc aatatctaca agattcttat tgtgataata ttcactttga
     1741 tatctaagat tgtgaataaa agacaaaaaa ataaaaaagc tagcgttttt ttacacagct
     1801 agcttttttt aggtataaca cttatagaaa ctgtttttac atttgcgcct tttttgctga
     1861 ctctttacaa tccggacaga gtccatacaa ttccaaccga tgttcattaa tctcaaaatc
     1921 agtcagtctg ctcgcagcca tttctatatc ttcaagccct gggtaatgaa aatcaacaat
     1981 cttaccgcag ttttcacaaa ttacatgata atgacgcttt gaactgaaat caaatcggct
     2041 agaagcatcg ccgtacttca tttcttgaac aaatccaatc gaagtaaata agcgaaggtt
     2101 attgtaaaca gtcgcaacac tcatatttgg aaaccgatgt tcaagtgctt tataaatctc
     2161 atcagccgtt ggatgggaat gatgttcaat caaatattct aaaaccgcgt accgttgagg
     2221 tgtgattcga atgttctctt tttttaactt ttcgataacc tcttcgacta acttattttc
     2281 catgtaaaca ccccacgttg ttattctcgt cttatcatac tatttttaca ttttaaaatc
     2341 cacttaacat gaaaagaaaa ttcaatttta ttgtatttta atgaaaaaag acgtaaaaca
     2401 gtcgcgttca ctattttacg tctctattca aataattttt ctcatcataa atttttgtgt
     2461 gttcttttca tctttaaaag ttttttacat aaacctatct ggagaaaaac caagtgcctt
     2521 atatctcttg gtaaagggct gtagataaat aacgttcacc attgtcggga accacagcta
     2581 ataccttctt gccttttcct aattcttttg cgacttttag cgcggcagca attgcagcgc
     2641 cagatgagat gcctactaaa atcccttctt ttcgcccaac ttctctagct gtttccatcg
     2701 cttcgtctcc actaatagaa aggactttat catacacaga agtatccagt gttttaggaa
     2761 caaaacctgt accgattcct tggattttgt gaggtcccgc ttctttccct tctaaaatag
     2821 cagattctgc tggttctacc ccgattaatt cgattttcgg atacacacgt tttaattctc
     2881 ggccagcgcc agtgatcgtt cctcctgtac cgatacctgc aacaaacgcg tccaagccat
     2941 ttactccaaa tgcttgatgt atctcaggac cagtcgtctt ctcatgcaca agaggatttg
     3001 cttcattttc aaattgtaat ggtaaaaagt agccgttttc ttttgccaaa cgttctgctt
     3061 ctctgattga tccagaaata ccatccgcac caggagtcaa aatcaactct gcgccgtacc
     3121 ctttcatcaa taaacgtctt tcgatactca tcgtctcagg catcacgatg attactttat
     3181 atccttttgc aactcctacc atcgacaatc cgattcctgt atttccagaa gtaggttcaa
     3241 tgatagtatc acctggtttc agtagaccat cgtgttctgc tttttctatc atacttaaag
     3301 caatacgatc ttttacactt cctcctggat taaaaaactc aagctttaca aaaacatctg
     3361 ctgaatcttc tggtacgatt ttatttaatt tgacgattgg cgttttgcca atcaattcgg
     3421 tgactgagtt atagatagct gtcatcttca attcctcctt gtctttgctc ttcttactta
     3481 tcataataca atctgttcat ttatggcgag tgattcgtct ttgactagca aaaaaaagac
     3541 aaagatatac catatatggt aatt
//
LOCUS       DACDBG010000150         3508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDBG010000150 DACDBG010000000
VERSION     DACDBG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.153"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IVZ90_002765"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IVZ90_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IVZ90_002766"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IVZ90_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8263750.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IVZ90_002767"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IVZ90_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IVZ90_002768"
     CDS             complement(2143..2373)
                     /locus_tag="IVZ90_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8263751.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IVZ90_002769"
     CDS             complement(2603..3421)
                     /locus_tag="IVZ90_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8263752.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT         1140 a          615 c          586 g         1167 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactt
//
LOCUS       DACDBG010000151         3484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDBG010000151 DACDBG010000000
VERSION     DACDBG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.154"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(453..1427)
                     /gene="sgrA"
                     /locus_tag="IVZ90_002770"
     CDS             complement(453..1427)
                     /gene="sgrA"
                     /locus_tag="IVZ90_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8263753.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1623..2462)
                     /locus_tag="IVZ90_002771"
     CDS             complement(1623..2462)
                     /locus_tag="IVZ90_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8263754.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2647..>3484
                     /locus_tag="IVZ90_002772"
     CDS             2647..>3484
                     /locus_tag="IVZ90_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8263755.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMK"
BASE COUNT         1155 a          722 c          517 g         1090 t
ORIGIN      
        1 tctgcttgtt gaatcaccga attcatctta gccagggtgt agtttagatc taaagcgata
       61 tccttcacca catataattt ctcgtctctt tcataacaat agttcaaaaa ttgatatact
      121 tgcctttcat tgatatcaat aaattcttca aacaaagtac atactccttt ttatctatta
      181 tttagactta ttccgacagc tacatgaata acgtacaaat agattcgtta ctgatttttc
      241 agagatacta ccatgcgcag tgtgcattgt caattattct tgctcttatt ttccatttgt
      301 gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct
      361 acaacttcgc caggcaatac agcactctcg aatgaaatat tcactcaaaa atctatcgtt
      421 ttattcaatt ttagatctgt tgcttttatt ctttatttca ctcgatggaa gagagaacga
      481 aaatagcttg ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc
      541 aaggttctac taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt
      601 tcttgatgat caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg
      661 ctccctgcat ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta
      721 tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc actactcatg
      781 cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct cgtgcttcct
      841 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta
      901 ctttcactgc tcgtactctc tgtactgctt tcaccgctcg tgttttctgt actgctttca
      961 ctacttgtgc tatccgtact gcttggcggt ccaacaactg tccaactgct tggtttcaaa
     1021 ttctccgtga tcgtcatctg tccatcataa aaaacaatca aatcaacttt atctttactc
     1081 ttgatctcat caatcgatag ttctgcagaa atcattattg tatactcaat accagcggtc
     1141 cattgtacgt cttttaaata aacttcccca ataacttttt ctgaacttcc attaccatcc
     1201 caaccatatg ttagaacagc atgtacatca ctagcattca cgcttttgtt tggtttgact
     1261 ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc gatcagtttt cacagtaaat
     1321 gaaagattct catttgcccg ttcatttgca gaagtactga ttccaatcat tgctgcaatt
     1381 atactcagcc caagaataac aaataatacg gtcgctgttt tcttcatttc tatcactctc
     1441 catataatta ccttctattt gataaaggaa ttatataaaa tgaaaatgat gagaacacga
     1501 cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt gtttactttt
     1561 tcgtttaatc aatgacagac ttaaaaaagc aagaccgata tcagccttgc tttcttcatc
     1621 ttttattgat aaacagcagt gtatttcttc atccgctttg ttttatatgc atagagtaac
     1681 aaaccaacaa tcaagactaa caagaataat gtagttgata tagatgcttc agccccaaag
     1741 tttcccccag taattattgt attttgcgat tggatcagag tggagaaaaa accttgttct
     1801 tgcgttccgc tgactggtat acccaaaata gctccttgtg tccaattcca agtaatatga
     1861 gcaccaatag ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa aaagcaacct
     1921 gctaaaaaga tattgaaaac tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga
     1981 gcaaataatg cagcagatcc aatcattgct attaacggac caaatctaag catctgatgc
     2041 tgaaaaaagc cacggaataa aagctcttct gatgccccca ctgcaataaa taaaataaaa
     2101 ctcgttatca gcggcacaat gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa
     2161 tataatggac tagtaataag cagaaacact ccaattccaa caacgagacc tcccgctaaa
     2221 tacccccagt cttttcctcg aacagaaaca ccataatctt ctattctttt tccttcgacc
     2281 ttactagcaa gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt accggcaatt
     2341 gctccaatcc attcactatt tccaataaac atatccgcaa gtgcattatt gacaagaatt
     2401 cctaaaacaa aaccaactaa gactattaaa acagatccaa tcaaccgaat aatcctgatc
     2461 atctttcatc cctcctttat attaagttta acaacccttt tctttcttgt ctcgtgaaaa
     2521 ctatgtaaat gttctccatc atagagagaa tagattttta cgaactaaga atatctacag
     2581 caaccttacg aaaatgaaag actgtctccc taattcttct gttagactgt ttttaataag
     2641 gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca
     2701 attttactat tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga
     2761 acacttacag ttcaggattt ttatgataac ccaaaagtta aatcaaataa tcaaacacta
     2821 ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat acggaaaaga gatttcttca
     2881 aaggaagtaa caaaagaata taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa
     2941 caattaaaag cagttggcca aacagaagag acatataaga aactctttaa acagatgctg
     3001 gctttccaat acggtttgaa agccaatgta aaattaacag ataaagatct agacactgct
     3061 tggaaagaat tctatccaga agttagtaca cagatcattc tattttcgac taaagaagaa
     3121 gcagataaag caaaaaaaga agcaaatgaa ggggaaaatt tctccaagct tgttcaagct
     3181 tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa tgaattttga ttcaaccaat
     3241 ccagagattc ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct
     3301 gatatcattc ctgtaacaga tccaacgact tatcagcaaa gctattatct agtaaaaatg
     3361 gtaaaaaaac aggataaagg ttctaataaa gacaaataca aatctgagct agaaaaaatt
     3421 gctaccgaag caaaaactct tgatacggaa ttcatggata aaacaatccg taaagtgatg
     3481 aaaa
//
LOCUS       DACDBG010000152         3401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDBG010000152 DACDBG010000000
VERSION     DACDBG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.155"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IVZ90_002773"
                     /pseudo
     CDS             75..470
                     /locus_tag="IVZ90_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IVZ90_002774"
     CDS             790..1104
                     /locus_tag="IVZ90_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263756.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IVZ90_002775"
     CDS             1208..2386
                     /locus_tag="IVZ90_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8263757.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IVZ90_002776"
     CDS             2789..3082
                     /locus_tag="IVZ90_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8263758.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1160 a          494 c          672 g         1075 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata a
//
LOCUS       DACDBG010000153         3282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDBG010000153 DACDBG010000000
VERSION     DACDBG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.156"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..146
                     /locus_tag="IVZ90_002777"
     CDS             <1..146
                     /locus_tag="IVZ90_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP8263759.1"
                     /translation="VFPSENDKSFTTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQEN
                     GSL"
     gene            172..717
                     /locus_tag="IVZ90_002778"
     CDS             172..717
                     /locus_tag="IVZ90_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263760.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            718..1284
                     /locus_tag="IVZ90_002779"
     CDS             718..1284
                     /locus_tag="IVZ90_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263761.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            1299..1547
                     /locus_tag="IVZ90_002780"
     CDS             1299..1547
                     /locus_tag="IVZ90_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263762.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            1567..1806
                     /locus_tag="IVZ90_002781"
     CDS             1567..1806
                     /locus_tag="IVZ90_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263763.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            2177..2458
                     /locus_tag="IVZ90_002782"
     CDS             2177..2458
                     /locus_tag="IVZ90_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8263764.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            2448..2789
                     /locus_tag="IVZ90_002783"
     CDS             2448..2789
                     /locus_tag="IVZ90_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8263765.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         1258 a          373 c          721 g          930 t
ORIGIN      
        1 atgtttttcc aagtgaaaat gacaaaagtt ttactacgaa tttgaaaaac gcaggaaaaa
       61 caattggtat tgaattgtta gaccatatca tcgtaacaag taaaaacaca agctattact
      121 catttcaaga aaatggttct ttataaaaat tagagaaaag aggaaaacaa gatgggcgaa
      181 agaacacaag cgttaattac agtcaaatat ggtaacagca aaacgaaagc aagcgtgtca
      241 attcattatc agtggggata tggtcgatac atgttgatgg acgttttgaa tcaaggaata
      301 tatttaatca ataaagcata tgacttacga gatgaatcaa gagaagaatt agcaaaagga
      361 atctttgata gaagtacagg agttatcaat tttgttacct atgaatctgg agtgagtgct
      421 tatgatgaaa cagggtttgg tgggcgctat gaaccttatc aagaaacgat tgaagatgtg
      481 atgaagtttt cagataacaa tgatggctat gctcatttag agatggtgtt cgatgaaaat
      541 ggatttagtc tagatgataa aaccagattg cgattgtttg attttgaggg tcaacagata
      601 ggactaaaca agttttgtga aagcggaaaa gagtttgcta cgaaagattt tattaaggga
      661 tacagattat tgctagaaaa ttatggaata aacctagaag aagagagcgt gaaataagtg
      721 tttgaaaaaa gaaaagacca gttccctaat ttagaagcga aaagtcaaaa agtgattgat
      781 ggtgaaatga ttgaattatt ccaaaaaagt tatggaacca tagaaactac cgcaagttgc
      841 aaatactact ttcaaggaaa gagaaacacg tttaaaggat ttgccgattc tgatgaagaa
      901 ttggcaaaga aaatcattga ttggcttgtc agcagtgaac acgtagcaag agaagatacc
      961 gttagaacaa cgattaatgg ggaagtccta acaggtacta gatgtgtttg ttgtaatcat
     1021 gaatttgtag aagatgaagg ttgctaccga atcaatgaat atggtgattt tatttgccag
     1081 agttgccacg agatagaatt tccaaccgaa agaagttggt ttgaatacat gctacaagat
     1141 tatgagatgg aaaaagaaaa tggtgaagta gttttttgta ccaaagagga attgagtaag
     1201 agaagcgatg atgaaattga agattggtgt gtgtggttag taggggcaga tgattgcgtt
     1261 gcatattaca caacagcaga ataggagtgt gaatcagcat ggaaggaatg gattttctag
     1321 atcatgaaga tttggttgat tttggttata catggaaagg aatggtaggt attagcagaa
     1381 gtcttgctaa tgccttttac gaaagaaatt atgcagtata tgttctttat gatgacgata
     1441 cagaaagcct tgttgacgag gaatataaat tggatttaga gaatgtttta tatggaatag
     1501 aaaaggaaga tttagcaaaa tatatttttt catggttggg tcaataagtg aaaggaagat
     1561 agaaacatga caaaatttaa aagtaagggt atgcaatggt gttcttatta ttatgttgga
     1621 aatgatgaag atttattgag aaaaacccaa cgaaaaggtt atccagataa gatattgaaa
     1681 caaataggtt attacatgcc agaagagagg atagaaaaag aattttgtga ggttcttttt
     1741 gatagtgaac agctattagt tttgttaacc caaaataaat tgatttgcca atttgaaaat
     1801 aattaagatg aggtggtgaa ccatgaatga agataagatg gtattaagtg aatatgctag
     1861 attgagaact attaattctt tagcatctca tttaagttgt tctttcctaa caaaggtgct
     1921 aaaattaatt tacaaatgtg aagattttgt acaagagaag cacaaagaca agcctactgc
     1981 gaataggctt gtcttttttt cattcattac gattccgtat gtgtgggtta gcgacaattt
     2041 gttcaattac cgtatgatat agcatcttaa tttgattaga ttacttgcaa tgtagtttat
     2101 taagagtata atataatcat aaatgtggac aatttgataa ataatgagtg ttttatactc
     2161 aaaaggagtg gatgaaatga aggtcaaaaa tccaactgaa aaaagcagaa tccaagtcgg
     2221 aatagacaag aatttaaaag agaacgcaga aatgatattg gaagagctag gtttaaatcc
     2281 tacaacagca ataacaattc tttacaaaca agtagtcgct agaggtgagt ttccagtaga
     2341 gataaagcta agtgaagagg agaagcaagg aatcagattg caacagctaa ctaaggacat
     2401 gccagtagat gttctagata cagatgaaaa gttagaagag tggttcgatg aagcctaatg
     2461 agatagtaac ggtatatgtt gcatttacag ataaaaatgg tggtaaaaga agacctattt
     2521 tggtggttag tgataaagaa gatagagtag aattctttgg aatcacaagt caatatgaga
     2581 aaaaatcaga ccaaataaag agggtgtatt ttcctattgc agaatgggaa aaagcaggac
     2641 ttaaaaagca atcatggatt gacgtagggt ctttaaaggc tattcccaaa agccgagaga
     2701 acatatcatt caaaaaggtt ggtatgttat cagctagtga tacgaaaaag ctaaataggt
     2761 ttatcgagaa attacaaaga aaagtctaac aaggcttttc ttttttgttt atatgtatat
     2821 taagatatac gtttatataa acgtatttat gtatatgaaa atatacgtaa atatatacgt
     2881 gattataaac attaagaaaa cgttaagaat tgttaaatag tgatatttag taatatacga
     2941 attataagat atattttata tagaatgtat gtaaataaat aaaatgtctg acggatattg
     3001 aatagcaata aataacaata attcagacgg atacagtgag tgaagtagct tgctacgaac
     3061 gaacaggcag aatgaaacgg cagtttcatt agagcaagtc gcttgcgacg tagcgttacc
     3121 gctgatgtaa ctggcaagtt acatagagag agtttgcttg caaacgaacg ttacctactt
     3181 gaatagaaat aaaaaaataa catattagtg aattttgaat aggagccaca actcaaccaa
     3241 ccgcttgcgg tgggtgagtc ccacggagga aaagacgaac ga
//
LOCUS       DACDBG010000154         3276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDBG010000154 DACDBG010000000
VERSION     DACDBG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.157"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..744)
                     /locus_tag="IVZ90_002784"
     CDS             complement(<1..744)
                     /locus_tag="IVZ90_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP8263766.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILST"
     gene            complement(716..1915)
                     /locus_tag="IVZ90_002785"
     CDS             complement(716..1915)
                     /locus_tag="IVZ90_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8263767.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2045..>3276)
                     /locus_tag="IVZ90_002786"
     CDS             complement(2045..>3276)
                     /locus_tag="IVZ90_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8263768.1"
                     /translation="NITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVK
                     RINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVE
                     GKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFD
                     GHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDL
                     GKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNA
                     RKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTG
                     DIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIIS
                     SFNILRRNPKELLIDNN"
BASE COUNT          981 a          643 c          510 g         1142 t
ORIGIN      
        1 tgtgctaaga ataaatccat tgtcgtcgca tgaagtatgg gcagaataag caaattgttt
       61 ttctcgttcg cctttgacgt agtagccgct ttccggatca gctttactta tttttctttc
      121 tttcagcttc acttcctttt ctaagtccaa agtaaaaggg cgttttccag cctctattcg
      181 ttgacgattg atctcatctt ctaattcatc ctgataggct ttcgcttcat catgggtcat
      241 ttctttcgta aacttatgtt tattggcgtt agctttgatg tgggtagaat ccagatacaa
      301 attatcctct gttacaaaac ctgctttaac tgcttgttct aaaatatagg caaaaatatc
      361 ctcaaacacc gtcgtttctc taaatcggcg aacataattc tttccaaaag ttgagaaatg
      421 ggggattttc tcctcgaagg agtagcctaa gaaccagcgg taagcgacat tggtatcgac
      481 ttccttaatg gtttgtcgca tggaacggat accgaataaa tactgaataa acaccaattt
      541 gatcagaatc actggatcga tacttgggcg acctaaatcg gagtaagtgg cttcgacgat
      601 tggataaata aattcaaaat caagcgcggc atcaatcttt cgaacgagat gatcttgagg
      661 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
      721 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      781 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      841 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      901 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      961 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
     1021 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
     1081 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
     1141 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
     1201 tcgaaatgaa aaaaacatct tctacaccac ctactttcag ttcatcaaat acttgcatcc
     1261 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
     1321 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
     1381 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
     1441 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
     1501 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
     1561 acatggaaag aaccttacct tcgatgtcgg agacatctcg ttttctatta ggaatcaact
     1621 caggttcaaa agaagcctcc cggtctctgg gaacatcaat gtttacttta ccaaagctcg
     1681 ttttaagcgt cttattgcca taaccgtttc gtcgattgtc atgtttcttt ggctctttag
     1741 aatgggcgtc ataccctaaa tggttattca attctccttg aagcattttt tcaaataagg
     1801 gaccaaacac atcttttaaa acatcttgca tatcctctac agattcgggt tgataagtat
     1861 tcaaaatgga ttcagctaac ttctcagcat caggatttct tttctttcta gccatcgtgt
     1921 aatcacctct tgatcttatt ttagaaaaat aaaaaccgtg aagcaaccac ccgcctagga
     1981 ttgatggtta cttacacggt ttatattaca ctctcatttt tggtgctaaa aagcaccgaa
     2041 aattttaatt attgtcaatt aataattctt ttggattcct tcttaatata ttgaatgaag
     2101 aaataatcaa tgatacaaga agtactaaac tcataaatat aactacataa atcatgaatt
     2161 taggtaatac attgatatct aaacttgata atgtcttgtt aaatccttca gcttctgccc
     2221 caccacctaa ttggctagat gcagattgtt tagctatttg tttagctata tcaccagtaa
     2281 ccttattcaa tatattattc ccaagcttgt cagctgtata ttgagctaag aaataggaac
     2341 caacgaatgc ggggattgat ataaatacca tctcaattag gaattgacca aaaatttcta
     2401 attttgatat acctaatgat agtaatactg ctatttcttt ctttctagca ttcatccata
     2461 agaataataa taatgaaact acaactcctg caaatattaa tgagccaaaa aataatttat
     2521 ttgaaattga gtaaatacct gatatagatt gttgtaatgc agggtaattt gatgaacttt
     2581 taatcaaatt atattctctc caatttatat ctaatttccc aagatctttt attacactat
     2641 caagattctt atcacctttt acaaagaatg ttgcatcttg gtatacagct gtatcttcgg
     2701 tattaccata aactttagca gctgtatgaa tatcagtgat taatgtattt tcgtatagtt
     2761 cttgtgctgc acttactcca ccgctattat gcccatcgaa tagaccttta atttctactt
     2821 ctactgtttc atctgcacct ttctcattat cagcatcaaa taaattagat tttattttta
     2881 tcttatctcc aactttaagg ttattttttt cagctaaatc tttatgcatt aagattttat
     2941 ttttatcttc attttctaaa tgctttcctt ctactaattt atatgcttct gatacaaatt
     3001 ttgtttcttt tgttgagtcg ttaaccccag ttaacataac tgttctttta aaattctttg
     3061 ctctttcagg tgattgattc gcaagagtct cttgagtttc aataatatca taatcaacta
     3121 aatctgcaac actgtttatt ctttttacat aggagtctat actatctgtt tgagatatct
     3181 ttttaatatc ttcacctttt acatttcccc cacctctagg tgttcctgga tttacttgtc
     3241 tatttatctc catagaaaaa ctatttgtta tattag
//
LOCUS       DACDBG010000155         3228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDBG010000155 DACDBG010000000
VERSION     DACDBG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.158"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(355..471)
                     /locus_tag="IVZ90_002787"
     CDS             complement(355..471)
                     /locus_tag="IVZ90_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8263769.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(947..1825)
                     /locus_tag="IVZ90_002788"
     CDS             complement(947..1825)
                     /locus_tag="IVZ90_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263770.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(2148..3173)
                     /locus_tag="IVZ90_002789"
     CDS             complement(2148..3173)
                     /locus_tag="IVZ90_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8263771.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         1093 a          604 c          533 g          998 t
ORIGIN      
        1 cctttgatta tgtagttacg tttccattct tgactaaaaa tgtttcaaaa acgaacaaag
       61 gtgttaaaag gtaaagtgat caatactact ggtcaaaata agaggttgaa agggaattga
      121 aaaataacta cagataatac ttttataaac gtacgtgctt tgtggttctg tagttaacta
      181 tataactgga attagggaat ggtatcaatt tgaaaagaaa gattctacca atttatcata
      241 tgttaaaaat ggaaaatcaa cagcagttct ttcggaaacc ttacacaaaa accgattatt
      301 gaaaatagcc gaaaaacgtt tttcggctat ttttttaatt aagtttcctt cttcttacct
      361 tttcccacta ttccgtttca catctttgat gatcttcagc gcacgttttc gtgttttgat
      421 atttgggctt ttcaagcggc ggtatgcact tgagatatct gttttttcca tatggacgac
      481 ccactttctt ctttgattgt agtcatgcta aaaaacacaa atcgaaacaa ttacgatttg
      541 tgttttaaat ggtatcaaaa gttaaaatga aagtcaactt gttaaaacag tcgatcagac
      601 tagctgattg actcctatct ctggaaataa attttcatta ttacgtttta gctacctaaa
      661 aaaggccctc ttgtccgtta actttaatat ctattgttat agctagttta cctattgaac
      721 gtactgtgtg ttcgatagaa cactaaaaaa gaggctggga cagaagcgtt taactccaag
      781 aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc
      841 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
      901 tttacttttg tctcacgctc ttttaaacta tttcgaagta ctaaacttat gaagtaagaa
      961 tacctaagcc agagccaatg tgctcgcctg caagcaattg ctcttttgct tgtcgctgta
     1021 aactcgtact ttctggataa tcatgtattt gggcatctac tcgtccaaag atccaaggga
     1081 ctccactatc gtcctgttca tcaactgata ataatcgata atcggctgca aaaagacaga
     1141 tttttgactc ttttttcaac catttagtaa gattgggtga tagtaaccaa gtatctgtgt
     1201 acatcacttg gtaatggtaa tcaggaaaat aaagttcaaa aaaacgaatg gcactttcgt
     1261 aactatctct tatcttattc tgagataaat ctgcatcgga aggaatatga agagaaaggc
     1321 actgctttcc ttctaatctt agatccgtgt accctttcgg aaaatatgtc atttcgtact
     1381 ccaattcacc aatccgaaag atcaagccac tagtgtaatg ccaaatccaa aaaccacggt
     1441 caaagcgata gcttcctgtt acttggaaat gttcatgaat aaatctagta aaagcattca
     1501 ttgttgccaa atagatagtt cttggaatct tcaatggttg ataaattttt ttccaacttt
     1561 gagaagctgc atatagataa atagctaatg ctgcaaaagg ttcctcttca tagactttgt
     1621 ctattttctt tgcagtcgcc tttgctttat taggatccat tagttcatga ctatattgct
     1681 caaacacctc atcaaggatg ttttttgctt gtacaatcat ttgctctata acgactttgg
     1741 gaagagttaa agtttctgcc agaatttgga tttcattgct tattttcttc atcaaatttc
     1801 cccttttcat atctttgaac ctcattgttt aaatgactga ttatccggac accatatggt
     1861 ataaacatac ttttattgta cactaactat aaaacttaaa ctttctatga agaaagaata
     1921 tctgtaagac tttcaaggaa agcattgacc atctcaggaa agtttattca gttaccatca
     1981 taaacaaaat tttttgatag aataaatata aaattttttt gaaagttcct ttccacattt
     2041 ttgtgtgaat ttgacttaat gctgagaatc tggcttttga acaccttgaa ttagaggttg
     2101 tgacaaaaat cttgtcataa cctcttttgg cacatatatt tgtccttcta ttctaatttt
     2161 tgacgaacaa ccgttaaata atgttgcaaa ccaaccgtca ttacccacgc aacacctaaa
     2221 ccgatgaaac ttcctatgac gatccctaaa aaacgatatt cgatcaaaga aatcaagtat
     2281 gctccacgaa taagtgtaga tagcatcaac gccatcggat taagaaaaat attagcaact
     2341 gcataatttc tagcaactag aaattgtgcg attccatata gaaaagtgat cagaaaaatg
     2401 gtctgcaact gcgtaaacgg cacaaatgac aagaatgccg acacacataa cccacccatc
     2461 gatccaatca gatattggac ttgtcgatgc ttcatcgcat ccaagttttc tgctagcaaa
     2521 atcgatgcac atgacaaggt caaccaataa ggattatgca gattcaagcc gtgactgaca
     2581 taaacggaaa gaaataaggc agcgctgtag aaaacagaat caatgatcac taaaggctct
     2641 tcgtgaaatc tttcttttaa agttgctttt tcaattgtct gctcaggccg tgaatccagt
     2701 aattttgtaa tcactgccgc aatcagggca aaaacgacac ccataaaaaa ataaagtgag
     2761 acgatcggta attgagcgat tggcagctgc atgctcgttc ccatggcaga aagcatcgca
     2821 aaaaacaagc cacctggttt agaaatatgg aataatcgca acacgaatct gctggaaaaa
     2881 gcaaccaaag ctacggcaaa tggttcaagc cataaaacat aagtcgataa gatccccaaa
     2941 acgaaagcaa ccaacagtcc gcaaccgatc agaaacatac gtttcagcaa atatttcatc
     3001 ggtatgtttt gataatagaa aaaagtaaag atccctaatg atgaaaaact tgaaatcaaa
     3061 aaatcatgat ttatgtaacc taaccataat acaataaaga taagtagcat cagattcatg
     3121 atacgaaaag gattatcttg tattttattg aaacgaaaat aagtcttgaa caatctatcc
     3181 ctccttccta gtaattggaa aaaaagctga accaagatga aatgccaa
//
LOCUS       DACDBG010000156         3211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDBG010000156 DACDBG010000000
VERSION     DACDBG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.159"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..821
                     /locus_tag="IVZ90_002790"
     CDS             <1..821
                     /locus_tag="IVZ90_002790"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8263772.1"
                     /translation="TMVDSNGKPILNKYGKPKKVVKMPIDNSVTLSIQRLRHLASLGN
                     ESNKQIVDRYFSNQPEPLTINEQMELLQTGINLHSIYMVDENENEIITAPAIARWVIN
                     KHTLEVTARINPEYLEWFSEVVNQYILFNLRDFVKLKSKYSMILFLELSTFWNTGMYY
                     KRINDLREILGVNPTRNNRDFINNVLKPAIAALKEYFPELSLTVEKNSSDSRKITHVR
                     LKFDKLTITDMQERLRSSEGASLVEYQQELENMEGEEKAVNDPTPEIDLSNVEW"
     gene            935..1303
                     /locus_tag="IVZ90_002791"
     CDS             935..1303
                     /locus_tag="IVZ90_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263773.1"
                     /translation="MENTNQTPNTAPTNQTSPAERKADLIRLYSRLEGMKLQHVPTAP
                     KAPDGFPKSAESLEFFECKVCGSTFQTILRESVASGCPKCEHDRLGILADHLPEPDKN
                     SCLNNRKKPVTVMAMGFLCI"
     gene            1477..1785
                     /locus_tag="IVZ90_002792"
     CDS             1477..1785
                     /locus_tag="IVZ90_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263774.1"
                     /translation="MKEMKMKQVIYPVIIGVLAIALIILGVNCYNLSKENNQLETKNN
                     KQEIQIAKQAYVISDYKTYFRENYVDTVKYRSHFTDKQILNVYREALRDGKISDEGAN
                     "
     gene            2048..2368
                     /locus_tag="IVZ90_002793"
     CDS             2048..2368
                     /locus_tag="IVZ90_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263775.1"
                     /translation="MERFLTKNRKGGGKLEKKEDLLNAFKNLDEKDQLKALLRLHDIT
                     YSDIAERLTVKKGEREGQKGVSLVTVQKAFQSKTNLESDRWKEIVKVATKLIEERQND
                     QNKA"
     gene            2499..2708
                     /locus_tag="IVZ90_002794"
     CDS             2499..2708
                     /locus_tag="IVZ90_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263776.1"
                     /translation="MIRKLNNIKLSLAILTLFFGIYNNSRMALFFLTLLIFMNLIIHN
                     FKELENTAPAPSQGNQESEGMPHAW"
     gene            2698..2970
                     /locus_tag="IVZ90_002795"
     CDS             2698..2970
                     /locus_tag="IVZ90_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263777.1"
                     /translation="MLGSFKKDKEVKTRGALTPSQQNKLGTKATFHPVRDQANQKELI
                     GVCVGFTSEKFFPIIECKIHGITTREVARKVVKWHTSQTKTNHKKR"
     gene            3018..>3211
                     /locus_tag="IVZ90_002796"
     CDS             3018..>3211
                     /locus_tag="IVZ90_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263778.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAF
                     FIVAVNSSGGDKKEAASKDTT"
BASE COUNT         1212 a          577 c          647 g          775 t
ORIGIN      
        1 taactatggt agatagtaac ggcaagccta tcttaaacaa atatggaaag ccgaaaaagg
       61 tagtaaaaat gccgatagat aactcggtta ctttgagtat tcagcgccta cggcacctag
      121 caagcttagg aaacgaaagc aacaaacaaa tagttgatcg ttacttttca aaccagccag
      181 aacctctgac aattaacgag caaatggaat tactccaaac ggggataaat ttacactcta
      241 tttatatggt agatgaaaat gaaaatgaga tcataaccgc gcccgctata gcaaggtggg
      301 taataaataa gcatacgcta gaagtaacag cgcggatcaa tccagaatat ttagagtggt
      361 tttcagaagt ggttaaccaa tacattttat ttaatctaag agactttgtg aagttaaaaa
      421 gtaagtactc catgatcctt ttcttagagc tttctacctt ttggaataca ggaatgtatt
      481 ataaaaggat taatgattta agagaaattt taggagtcaa tccaacgaga aataacagag
      541 attttataaa caacgttcta aagcctgcta tcgctgctct caaagagtac tttccagagc
      601 taagcctaac agtggaaaag aattctagcg actccagaaa gatcactcat gtaagattga
      661 agtttgataa gctaacaata accgatatgc aagaacgcct aagaagcagc gagggagctt
      721 ctttagtaga gtaccaacaa gagttggaaa atatggaagg agaggaaaaa gctgtcaacg
      781 atccaactcc agagatcgac ctttccaacg tggaatggta acacctgtca gcaatctaat
      841 ccaataaacc aaagtacttg taaggcgcga gggctccttt tagagccttc tagtcctata
      901 ccaaaccaag caaaataaag gagctataac caacatggaa aacactaacc aaacaccaaa
      961 tacagcacca actaaccaaa ctagcccagc cgaacgcaaa gccgatctta ttagattgta
     1021 ctcacgcctt gaaggtatga aactccaaca cgttccgacc gctccaaagg cgccagacgg
     1081 cttcccaaaa tcggcagaga gccttgagtt tttcgagtgt aaggtgtgcg gatcgacttt
     1141 tcaaacgatt ttaagggaat ccgttgcgag tggctgcccc aaatgtgaac atgatcgctt
     1201 aggaatttta gccgatcatt taccagaacc agacaaaaac agttgtttaa ataatcgaaa
     1261 aaaacctgta accgttatgg ctatggggtt tttgtgtatt tgagataatt tccaagggtt
     1321 atttatggat ttattttaaa aaatctatta aattatacta ccactggtag taatataaat
     1381 atatcgaaag ggtacgaggg ttaaagcgta atggcggaat gagtaccgtt ctcttttgaa
     1441 aataccaaac caaccaatta aaggagagaa agtaatatga aagaaatgaa aatgaagcaa
     1501 gtaatttatc ccgtgattat tggagtttta gctatcgctc ttattatcct tggtgtaaat
     1561 tgctataact taagcaagga aaataatcaa cttgagacca aaaacaacaa gcaagaaatc
     1621 caaatagcca agcaagctta cgttatcagc gattacaaga cttattttcg tgaaaactat
     1681 gtagacaccg tgaaatatcg ctcccacttc actgataaac aaattttaaa tgtttatcga
     1741 gaagctctta gagatggaaa aatcagcgat gagggagcta actagtggaa gagctcattt
     1801 tgctggctat gttattacag gaaaagaagc gtgaacgaga agcctaacag ctggaaagaa
     1861 tccgaatagg atatgaggaa gcagatcggg aaagagatca gaaaatagag gagctggaga
     1921 agcgtatcca agaactttcc aaaaactatg ataataacca agattgggaa caagagtggc
     1981 ggaaaatcgt tggtataaag gaaaatgaat acttagtgag aaagccgcca gaagaagagg
     2041 aagacccttg gagcgatttc taactaaaaa tcggaaggga ggtggtaagt tggagaaaaa
     2101 ggaagattta ttgaacgctt ttaagaacct agacgagaaa gatcaactta aagcgttatt
     2161 aaggcttcat gatataacat acagcgatat tgctgaaaga ttaaccgtta aaaaaggcga
     2221 gagggaagga caaaaaggtg ttagcctcgt gactgtacaa aaagctttcc aatcaaagac
     2281 caacttggag agtgatcgct ggaaagagat tgtaaaagtc gctaccaagc taattgaaga
     2341 gcgacaaaac gaccagaaca aagcataaaa aaagcccgtc catttaccgc tgaccaaagc
     2401 aataaaatga acgagccaca ccatttactt ttaggggtaa ccacacgccc taaaagctac
     2461 caaaccattt accaaataac cactaaggag atttagaaat gattagaaaa cttaataata
     2521 tcaagctttc tttggcaatt ttaacactat tttttggaat ttacaataat tccagaatgg
     2581 ctttgttttt tcttacgtta ttgatattca tgaacttaat tatccataat ttcaaggagt
     2641 tagaaaatac agctccagcg ccctctcaag gaaaccaaga aagtgagggc atgccacatg
     2701 cttggtagtt ttaaaaagga taaggaagta aaaacacgag gagcccttac accaagccag
     2761 caaaataagc ttggtacaaa agccactttc caccccgtcc gagatcaagc caaccaaaaa
     2821 gaactaattg gcgtttgtgt agggtttact tcagagaaat tttttccaat aatagagtgc
     2881 aaaatacacg ggatcacaac tagagaagtt gctagaaaag ttgtgaaatg gcacaccagc
     2941 caaaccaaaa ccaaccataa gaaacgttaa aaacgttcct aagagcgttt gaaaattaaa
     3001 ttaagggaga tttaagaatg aacaacaaaa acaccaaaaa taccaatact aaccacgaga
     3061 agagaagctt ctttgataag cttttcaaaa agaagaagaa tggaaaagta tctattttga
     3121 aaatgatcgg ctggatattt ttagcctttt tcatagttgc tgtgaacagt agcggcggtg
     3181 ataagaaaga ggctgcttca aaagatacca c
//
LOCUS       DACDBG010000157         3191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDBG010000157 DACDBG010000000
VERSION     DACDBG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.160"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..741
                     /locus_tag="IVZ90_002797"
     CDS             <1..741
                     /locus_tag="IVZ90_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8263779.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFY
                     SSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            916..1212
                     /locus_tag="IVZ90_002798"
     CDS             916..1212
                     /locus_tag="IVZ90_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263780.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1219..3171
                     /locus_tag="IVZ90_002799"
     CDS             1219..3171
                     /locus_tag="IVZ90_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8263781.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1171 a          486 c          631 g          903 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca
      601 aaaagtaata tagggattga taaccttata gaagttatta ctaataaatt ttattcatca
      661 acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa
      721 agacaacgtc ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac
      781 ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt
      841 ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa
      901 ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa
      961 ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat
     1021 tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt
     1081 actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt
     1141 gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat
     1201 agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt
     1261 gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat
     1321 ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct
     1381 tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt
     1441 tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat
     1501 aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct
     1561 attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt
     1621 gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa
     1681 tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt
     1741 tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag
     1801 ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc
     1861 attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat
     1921 attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt
     1981 ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg
     2041 tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt
     2101 tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct
     2161 gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg
     2221 gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt
     2281 aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa
     2341 aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat
     2401 ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg
     2461 gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt
     2521 tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt
     2581 aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg
     2641 ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat
     2701 tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga
     2761 gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa
     2821 ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca
     2881 aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt
     2941 tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt
     3001 tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac
     3061 aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta
     3121 gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag
     3181 cttggagtaa t
//
LOCUS       DACDBG010000158         3164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDBG010000158 DACDBG010000000
VERSION     DACDBG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.161"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..281
                     /locus_tag="IVZ90_002800"
     CDS             <1..281
                     /locus_tag="IVZ90_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8263782.1"
                     /translation="FSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKS
                     MDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(352..1662)
                     /locus_tag="IVZ90_002801"
     CDS             complement(352..1662)
                     /locus_tag="IVZ90_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8263783.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1674..3092)
                     /locus_tag="IVZ90_002802"
     CDS             complement(1674..3092)
                     /locus_tag="IVZ90_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8263784.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1002 a          655 c          492 g         1015 t
ORIGIN      
        1 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
       61 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      121 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      181 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      241 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata tatcattaaa
      301 aaaaataaag gtcaagctaa aatattattt aagcttgacc ttattataat ttcaacgatt
      361 gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata gataaatcac agcttttgaa
      421 acataagttt tgtactgctt cgattcgccc gaattctttt gcagttattt ggcaatgaga
      481 aaagcagaca tcttctatag gcttttcagg aaatgctact aaatcaaaag ctcgggaagg
      541 aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg atgtgtatgt ctttgacttt
      601 cgtcatttgc tttgattcag gtatttcttc agccactgct tgccaacttt ctggcatttg
      661 agcaaggtca ttgctatttt ttctattgta ttcattatgc cagtccatta tccaagaaaa
      721 aggaaattgt acatcaatca tattcagatt ttctaccctg atattttcga tcactccccc
      781 tctttctttt gaagatttca ttcgaatacc acagtctgac tgaaaaaaat tgatatcgtg
      841 aatatatacg tcactaactc cagcactaac ttcgctacca atagttacac cgtatcctga
      901 acggatttta catctagcaa cctcgattct tgatgatttt cgattgactc ttgcaccatt
      961 gccatctctg ccagacttaa tagcaataca atcatcacca caggccaaat cacattcata
     1021 gacacgcaca tttgttgaag aatcaatatc aatcccatct gtacttggtc catcattgtt
     1081 gcggatgatt actttttcta ctaatacatc gttagaataa gtgatttgca agttccaaaa
     1141 acctgatttc tgaaaaatca aatcacgaac aacaacatgt tctgcatgat acagtaaaca
     1201 agcgcgtgga cgtttaatag cataatccgc aatccaacga agtccctttt gatcgtattc
     1261 agctcttgtc ccgctttttt gatcttttcc ccaatacagc tcccaccaat ggtctccttg
     1321 accgtcgatt attccttttc cttctataag aatatctttc gccgagagaa cattcaaaat
     1381 cgctgctggc catttcattt ctacgccggc tactcgtgta tcgatttctg gaaatgcttc
     1441 gatatctgtt ctccccttga ggactgcacc ttcttcaaag ataagatgac tcttatcttt
     1501 taaaaaaagg gctccgacta aataaatgcc ttttggaata aatacaggta agtgttcttt
     1561 cgcaccttga tcgattgctt tttgcagtat tattgtatca tcttgttgag tgtcgtttgt
     1621 tttaaagttc aaagcattga taatcccttt ttttgtccgc aatacgtttc tccctatctt
     1681 tcattgattt ttttcaattc ttcaccaata aatacagttt ttgaacgatt cagtgggtaa
     1741 cctactagca aaatgattgc ggatacaaat aagcagaatg ctggtaacaa gtttgccatc
     1801 gtataaatcc tttcttgtac taaaactgat tggatggctc cacctgttgt agacgattgg
     1861 taaccaatga gtgccagcat aaatccgcct accgctcctg cacatgcttg tcctactttt
     1921 ctcgcaaaag aatttactcc atatactgtt ccatcttctc ttaagcctgt cttatattga
     1981 tgataatcaa tcacgtcagt aatgaatgcc cagaccatca aattgaataa accagatcct
     2041 aaagtagcga taaataaaag aatcagatat acccatgcat ttgttatatg aaggaaatac
     2101 atcagcaagt acatacatga agaagcaagt aacgcaaata cgcttgcttc ctttttacca
     2161 aataattttg tcagctttgt agcaaatggt gctaaaacta ctactgttcc gtaagtaaat
     2221 aaaagagcaa cagacattgc ttgtttatta tggaagtaat cattgaataa ataagtcata
     2281 ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt caacaatgac taaaataatc
     2341 agtgctcgat tagctaacaa accttttacc aactgagaag cagatacttt ttcgtttttg
     2401 acaactctta ctctttcagt agtcaattta caagttaaca tatatgcgac aaatgctatt
     2461 gcagcacatc cgattgaaat ccaaaagaaa tgattccctg aaacgacttg ttgtccactc
     2521 ttgttatggg tatacattaa aatcggaatg aagaatcctg tagttgatcc gccaatcgca
     2581 gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat taggatttga gctaattgct
     2641 gctgccattg ctccatacgg gatattgatt gttgagagaa aaatcccata aacaatgtat
     2701 gaaacaaaaa tataagcaat cttcaccgtc attggaagat gttggacgaa tggcaaaaac
     2761 aaaatgacag tcactaagca aaatggatac ttcatttttc tgatccaagg aataaatcgg
     2821 ccagtttctg ttaactctga gacatcacat aatcttccaa ccatcacatc agcaaacgca
     2881 tccaaaattc tcgaaatcaa gaataacgta cctacgattg cccctgaaac gccaagaaca
     2941 ttcgtataat aaatcatcaa gaaactattg actaaaccta aaataaaaca gttacctaga
     3001 tcaccgaaca tgtagcctat cttatcggaa atcccaaatt cctttttttc tttttttact
     3061 gtatttgctg taacagcatt ttgtgaaagc atttgaattc ctcctacctc ttctagtaaa
     3121 cgttttcaaa attataactc tttttttctt gtttttatat tcac
//
LOCUS       DACDBG010000159         3150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDBG010000159 DACDBG010000000
VERSION     DACDBG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.162"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IVZ90_002803"
     CDS             complement(<1..2303)
                     /locus_tag="IVZ90_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263785.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT          857 a          717 c          521 g         1055 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc
//
LOCUS       DACDBG010000160         3139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDBG010000160 DACDBG010000000
VERSION     DACDBG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.163"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            974..1240
                     /locus_tag="IVZ90_002804"
     CDS             974..1240
                     /locus_tag="IVZ90_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263786.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1298..1810
                     /locus_tag="IVZ90_002805"
     CDS             1298..1810
                     /locus_tag="IVZ90_002805"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8263787.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1807..2844
                     /locus_tag="IVZ90_002806"
     CDS             1807..2844
                     /locus_tag="IVZ90_002806"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8263788.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3004..3139
                     /locus_tag="IVZ90_002807"
                     /pseudo
     CDS             3004..3139
                     /locus_tag="IVZ90_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1127 a          548 c          625 g          839 t
ORIGIN      
        1 aataaaagac cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa
       61 aggcgttaca aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg
      121 tccgataggt catttctttg gtattgaaca tttatatagc tttattaatg gttttattga
      181 tgaccacgaa agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt
      241 cttcgctatc attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt
      301 agatttatat ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg
      361 caaataaggg ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg
      421 ccgagaaaac ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt
      481 ggacttgaat tgggattaat cccaagaaag taccaactca acaacacata aagccctgta
      541 ggttccgacc aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga
      601 gaaaacgaag aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt
      661 gatagtttat tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat
      721 gtaataagtt ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt
      781 tatattaata acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca
      841 aaataaacag tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt
      901 attcttcaag atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa
      961 ggagtcgctt tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta
     1021 cacaaaaaaa ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa
     1081 tccgaaattg agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa
     1141 aaagctatcc gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac
     1201 aaaatttaaa gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata
     1261 ataagttata ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg
     1321 ttactctacc gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg
     1381 gattcgccaa ttaagtcaaa ctttgggcat tccagaagcg actctttatt cttggcgtaa
     1441 aaaagctaga attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc
     1501 aagtgaagat aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc
     1561 ggaatattgc cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg
     1621 cttatctgcg aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga
     1681 agagaaaaaa caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc
     1741 ggaagccgct gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga
     1801 ggaagaatga tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat
     1861 cgcaatgggg cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac
     1921 gaacgatgga cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct
     1981 gtacccaaaa ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa
     2041 aaagaattta tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt
     2101 tatattgcat cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac
     2161 cgtggccgaa gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca
     2221 aatgaagttt ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac
     2281 cggttatatc tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa
     2341 tcagaagaag ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc
     2401 caaggacagc cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt
     2461 caagtcttac tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat
     2521 gacaatcctt attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat
     2581 aacggcttta aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat
     2641 aacgattctc atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga
     2701 cgacatctcg ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat
     2761 cctgaacgct ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat
     2821 cctttaaaag aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct
     2881 cgtcacccaa taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca
     2941 tagggaggtt ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac
     3001 accgaccatc gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct
     3061 acaacgatta aaggcatgga agccatccga ggattatata agaaaacccg aaaagaaggc
     3121 actctcttcg ggttttcgg
//
LOCUS       DACDBG010000161         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDBG010000161 DACDBG010000000
VERSION     DACDBG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.164"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..279)
                     /locus_tag="IVZ90_002808"
     CDS             complement(34..279)
                     /locus_tag="IVZ90_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263789.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(273..674)
                     /locus_tag="IVZ90_002809"
     CDS             complement(273..674)
                     /locus_tag="IVZ90_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8263790.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(846..2654)
                     /gene="uidA"
                     /locus_tag="IVZ90_002810"
     CDS             complement(846..2654)
                     /gene="uidA"
                     /locus_tag="IVZ90_002810"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8263791.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(2682..>3128)
                     /locus_tag="IVZ90_002811"
     CDS             complement(2682..>3128)
                     /locus_tag="IVZ90_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8263792.1"
                     /translation="KQILSAKGFEVEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIK
                     DLTEKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVP
                     QVKTYINTYDGKQETLEILVDKLMGLSEFKGISPIDAYCGLKDTFI"
BASE COUNT          947 a          593 c          410 g         1178 t
ORIGIN      
        1 ttaaacagaa taagtggaaa ggggactttt tagttagtta attttaaatt ttaagatcgc
       61 ttctatcaat ttaatttcta gctgtcttag catatactca tctacatagg taataaattg
      121 ccccgcctca ttaaatccat ttgtcaaaca taacttagct atataactat gataatgttt
      181 gagtatcatc ttgattgaaa ttggatcttc ttctagagca gattggataa tggatagagg
      241 aactaatgga tactgccgtt ttttggggtt gtctaccata agtcataccc catttttaat
      301 tgttcaactg ccttctttcg ttgataccag ataccacttg tactcatatt gaggatttta
      361 ccgatttcag tatcattaaa acctgcaaaa taataaagta ggataacctt tcttcttatt
      421 tccgataaat gatcaatggc tgccgataac ttttgatctg aaattaatat ttccattcca
      481 aataagaaat agacatcttg ttcatcaaaa tgagtttggg agtacttacc ttcatttgaa
      541 ttcgtcaatg gttcctcatt atctgttatg ttaatgtttc gttttcgaat agacttagtt
      601 tcgtttcgta atactttttt acagtaggca tcaaattgat gttgtataga tttctcattg
      661 taattttgtt gcatgataaa tatgctcctt ttttcccctt tccactaata caacaatcag
      721 aaagcgcctt tttttcgaaa aaactagaaa aaaatatcct tctttaaaca gaaaatcttt
      781 ttataaaaaa aaggagttgg caattaagca gccactcctt ttttatattc cttataaaag
      841 gtaaattatt ttttataact gaaatttggt attgatgacc atcgtttctt tagatagtga
      901 gcaatcattt taggctttct atctctagta aagattcctt tcttatttcc ttgcactctt
      961 aaaatccctt gacttgtttc aaaatctgca aaattccaca cttgttcacc aacaaaattg
     1021 tcacatttat ctattacttc gtgatttgct tcgtaataac ccacttgata ctcttctgta
     1081 aacattgttg gtactgtatc atgaagtcca gctactgtat ctgctccata ttctgtaaac
     1141 attattggcg tatcgggaca tctttccatc catccatcta gctctttttt caacagatat
     1201 tttgcatgtt ttaggtctcc acctgaaaca taccaaccat aatagcgatt taaacataaa
     1261 acatcgacaa tatctcctac tttacaaata tctggtgttg actttaagta agtaacgatt
     1321 gttacaggtc tcttctgagg gtcaagttct ttagtcaatt caactaaagg ttcaaaatat
     1381 tctttagcgc cttcagaatc cgaatctggt tcatttgcaa tagaccacat tacgacactc
     1441 ggatggttct tgtcgcgttc tatcaattcc ttaatcgcca ttttgtgagc attttctgtc
     1501 cgtatttctt tccaagtatc atgtttttct acgttgtcat ctaataaagt agccatgaaa
     1561 ttcaaatgta atcctactgc aggtacttca tcaataacta caataccttc tcgatctgct
     1621 aagcgcataa tttcttctga ataaggataa tgtgaagtcc taaatgaatt agcaccgatc
     1681 catttcatta aattaaaatc tttgatattt actgcttcat tcaaaccacg accattaata
     1741 tacgtgtcct catgcttccc aaatccctta aagtaaaaag gtttattatt aatcaaaaac
     1801 ttcccattcg aaacctgaat actacgaatg ccaaattctt ctgtgtacgt atcaatcact
     1861 ttattttcgt taattaaact aactttcata ctatataagt aagcatttag aggctcccaa
     1921 agtgttgggt tcttaagttg aatcactcct tctttcccct tagcttctgc tataagttga
     1981 gcttcttcgt catagattgc tattagaatt tctccggtcc attctgtatc gatattaaat
     2041 tctacatttg catctgaatc aattattttt gtattaatag tgacatcctt tatatagtct
     2101 ttcggagttg tatacaattt aacgggtcta ttaatgcctg cataattgaa aaagtcaaaa
     2161 ttaaccaaat tttctttttt tatgtctcca tcttcctgta tcgtttcttt caaaatccct
     2221 acaggtaaag tgctatcgtc aataacattg tttacagcca ctgtaattct gttacttcca
     2281 tcttttataa actgattaat ttctgattca aatggcgtaa accctccttt gtgttgatta
     2341 atcaattttc cattcaaaaa tacttttgct tcatgtgttg cagatccaaa acgaagaact
     2401 aatctttctt ctaataattt tttaggaaga atcacttctt tttcatacca cacccatcca
     2461 acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaagg aactgaaatt
     2521 ggataactct ctttttcaac cattggttct aaagcaagtt cttcttgtaa gtcatcattt
     2581 gtatctaact taaatctcca aagaccgctt aaatcaatta cttgcctcgt ctctgtcata
     2641 atgggatata acatgtaaat cactcctata taatttaaat attaaataaa tgtatccttt
     2701 aacccacaat aagcatctat tggacttatt cccttaaatt cagaaaggcc cattagtttg
     2761 tccactaata tttctaaagt ttcttgtttt ccatcataag tattgatata agtttttact
     2821 tgaggcacat cagctaaatg aaacggacac tgtacagata caaaaattgt tggaatttca
     2881 tgaatataaa atggaatatc cggagtacct ttacttgctg gccaaacaat tctttgaact
     2941 gtacctgggt taacattagc tatattaata atcaagtcat atttttcagt taaatctttg
     3001 attggtctct tagcagcata tattccagct atatttttaa ttttttcttc ttgcgataat
     3061 cctttcaatt cttcttcagg tgaaatgtat ttttcaactt caaatccttt tgctgacaaa
     3121 atctgttt
//
LOCUS       DACDBG010000162         3125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDBG010000162 DACDBG010000000
VERSION     DACDBG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.165"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IVZ90_002812"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IVZ90_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IVZ90_002813"
     CDS             1360..1992
                     /locus_tag="IVZ90_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8263793.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IVZ90_002814"
     CDS             2197..2424
                     /locus_tag="IVZ90_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8263794.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IVZ90_002815"
     CDS             2493..2759
                     /locus_tag="IVZ90_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8263795.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IVZ90_002816"
     CDS             2749..3018
                     /locus_tag="IVZ90_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8263796.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          941 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gcttt
//
LOCUS       DACDBG010000163         3085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDBG010000163 DACDBG010000000
VERSION     DACDBG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.166"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IVZ90_002817"
     CDS             complement(65..1285)
                     /locus_tag="IVZ90_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8263797.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IVZ90_002818"
     CDS             complement(1305..1910)
                     /locus_tag="IVZ90_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8263798.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IVZ90_002819"
     CDS             complement(1959..2891)
                     /locus_tag="IVZ90_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8263799.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          866 a          633 c          528 g         1058 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacat
//
LOCUS       DACDBG010000164         3052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDBG010000164 DACDBG010000000
VERSION     DACDBG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.167"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..442)
                     /locus_tag="IVZ90_002820"
     CDS             complement(<1..442)
                     /locus_tag="IVZ90_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8263800.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="IVZ90_002821"
     CDS             complement(500..1552)
                     /locus_tag="IVZ90_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8263801.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="IVZ90_002822"
     CDS             complement(1565..2503)
                     /locus_tag="IVZ90_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8263802.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1028 a          645 c          465 g          914 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca ac
//
LOCUS       DACDBG010000165         3034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDBG010000165 DACDBG010000000
VERSION     DACDBG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.168"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..621
                     /locus_tag="IVZ90_002823"
                     /pseudo
     CDS             1..621
                     /locus_tag="IVZ90_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1515
                     /locus_tag="IVZ90_002824"
     CDS             1069..1515
                     /locus_tag="IVZ90_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263803.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="IVZ90_002825"
     CDS             1517..1654
                     /locus_tag="IVZ90_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8263804.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="IVZ90_002826"
     CDS             1691..1984
                     /locus_tag="IVZ90_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263805.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="IVZ90_002827"
     CDS             1981..2205
                     /locus_tag="IVZ90_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8263806.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..>3034
                     /locus_tag="IVZ90_002828"
     CDS             2202..>3034
                     /locus_tag="IVZ90_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8263807.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RP"
BASE COUNT         1062 a          534 c          640 g          798 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gacc
//
LOCUS       DACDBG010000166         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDBG010000166 DACDBG010000000
VERSION     DACDBG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.169"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IVZ90_002829"
     CDS             177..359
                     /locus_tag="IVZ90_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8263808.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IVZ90_002830"
     CDS             462..746
                     /locus_tag="IVZ90_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263809.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IVZ90_002831"
     CDS             762..998
                     /locus_tag="IVZ90_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263810.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IVZ90_002832"
     CDS             1020..1274
                     /locus_tag="IVZ90_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263811.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IVZ90_002833"
     CDS             1356..1541
                     /locus_tag="IVZ90_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8263812.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IVZ90_002834"
     CDS             1608..2003
                     /locus_tag="IVZ90_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8263813.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IVZ90_002835"
     CDS             1993..2370
                     /locus_tag="IVZ90_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263814.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IVZ90_002836"
     CDS             2371..2766
                     /locus_tag="IVZ90_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263815.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDBG010000167         2932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDBG010000167 DACDBG010000000
VERSION     DACDBG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.170"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(552..2165)
                     /locus_tag="IVZ90_002837"
     CDS             complement(552..2165)
                     /locus_tag="IVZ90_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8263816.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>2932)
                     /locus_tag="IVZ90_002838"
     CDS             complement(2306..>2932)
                     /locus_tag="IVZ90_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8263817.1"
                     /translation="HITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEIN
                     SFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILY
                     SDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKELKME
                     KAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          941 a          524 c          511 g          956 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tg
//
LOCUS       DACDBG010000168         2908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDBG010000168 DACDBG010000000
VERSION     DACDBG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.171"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1419
                     /locus_tag="IVZ90_002839"
     CDS             <1..1419
                     /locus_tag="IVZ90_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8263818.1"
                     /translation="HIDFILLDLFCVQLAFYISYVMRQGDWNPYVIPIYRHMAIFIEL
                     ENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQDGNDYSRVSLFI
                     MGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRNLKEKNYGMYKL
                     SGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESVPYPKELIARCS
                     EMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIKRTIDILAGIIG
                     CIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYMDAEERKAELMK
                     ENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQFYNVLMGKMSL
                     VGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVVKLDAEYIRNWS
                     MGLDFRILFRTVKTVLHRNGSM"
     gene            1451..2587
                     /locus_tag="IVZ90_002840"
     CDS             1451..2587
                     /locus_tag="IVZ90_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8263819.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            2587..>2908
                     /locus_tag="IVZ90_002841"
     CDS             2587..>2908
                     /locus_tag="IVZ90_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8263820.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAG"
BASE COUNT         1027 a          339 c          636 g          906 t
ORIGIN      
        1 catattgatt ttattttatt agacttattt tgtgtccagc ttgcttttta tatatcatat
       61 gtaatgcgac agggtgactg gaatccgtat gtgattccta tttatcgcca catggcaatt
      121 tttatagaac ttgagaatct tacgattata tttttatttg aggcatataa aaatgtttta
      181 aaaagaggat attataaaga ggctgtagct tctataaagc aatcttttat gctgttactt
      241 gtgtgcagtt tataccttat tacaatgcag gatggaaatg attattccag agtttcttta
      301 tttataatgg gggtggtgta tggattatta acatatataa ttcgtatact ttggaaaggg
      361 ttgttgcatc accgcatgaa aaacggtgaa gatgtatcgt tagttattgt aacatctcaa
      421 aagattgcaa aaaatgtagt tcgtaatctt aaagagaaga attatggcat gtataaactt
      481 agtggtgtga ttgtagtaga cagagatatg tatggaacta ccatcggagg tgtcccagta
      541 gttgcaaatg ctgataatgc agcggaatat cttttacaga attgggtaga tgaagtattc
      601 atcaatgttg acgaaagtgt tccatatccc aaagaattaa tcgccagatg ttcggaaatg
      661 ggacttgctg tacatatgaa tcttacaaaa gtgtctgata gtacacgtgg aaaacaattt
      721 attggccgag ttggtgatta tactgttatt acatctagta ttaatgttat gacgatgagg
      781 caagcattta taaagagaac gattgacatt cttgctggaa ttattggatg tattgcaaca
      841 ggtgttattt ttatcttcgt agcaccagtt atttacatca aatcaccagg gccgattttc
      901 tttgcacaag aacgtattgg tcaaaatggt aagacattta agatgtataa gttccgtagt
      961 atgtatatgg atgcagaaga acgcaaagca gagttgatga aagaaaataa gatgagtaat
     1021 aatctcatgt ttaagatgga ttttgaccca agaatcattg gcaataaaat acttcctgat
     1081 ggaacaaaga agacaggaat tggacaattt attcgatcta catcattgga tgagtttcca
     1141 caattttata atgtcctaat gggaaaaatg agcctggtgg ggtttagacc ttgcttgaag
     1201 tcagaatatg agaagtatga ttttcatcat cgtgcgagaa ttgcgatgaa acctgggatt
     1261 actggaatgt ggcaggtgag tggtagaagt gatatcacag acttcgaaga ggttgtaaaa
     1321 cttgatgcag aatatatcag gaattggagt atgggattag attttagaat tttgtttagg
     1381 acagtcaaaa ctgtgcttca tagaaacggt tccatgtaag ataaagatac tatagaagga
     1441 gtaaaacgtg atggcaagaa aaaaattaaa aatctgtatg ctaggtcata aaagaattcc
     1501 atcaagagaa ggtggaattg agatagtggt agaagaattg gcaacgcgaa tggttgcact
     1561 tgggcatgaa gtgacttgct ataatcgaag tggtcatcat gttagcggaa aggaatttga
     1621 ccagcataaa aataaagaat ataaaggtgt aaaattaaag actattttta cattaaatat
     1681 aaaaggaatt gcagcaatga gttcttcagt atttggtgga attagagcag cattcggaaa
     1741 atatgatgtt gttcattttc atgcagaagg accatgtgct atgttgtggt tgccaaaact
     1801 ttttgggaaa agatgtgttg caacaattca tggtctagat catcaaagag aaaaatggaa
     1861 caagcttgct agtacataca tcatgttagg ggaaaagtgt gctgtaaaat ttgcagatga
     1921 aataattgta cttagtgaaa gcgtccaaaa ttattttgaa gatatatatg gaagaaaaac
     1981 aaggtttata ccaaatggcg ttaaaaaaat tgaaataaaa tcagcaggat tgattacaga
     2041 aaagtatgga ttaacaaaag attcttatat tttgtttttg gggcgattgg ttcccgaaaa
     2101 aggaattcgg tatctaattg aggcatttaa agatgttcag acagataaaa aacttataat
     2161 agccggagga agctctgata cagatgaatt tgcaaatgaa ctaaaggaat tagcaaaagg
     2221 tgatgagcga attatattta ctggatttgt acaaggacag gaattagaag aattatatag
     2281 taatgcgtat atctatacac ttccaagtga tttagaagga atgccattaa gtttgttaga
     2341 agcaatgagt tatgggaact gttgtatagt ttcaaatatc tcagagtgta cggaagtagt
     2401 agaagacaag gctatgattt ttaagaaatc ggatgtaagt gatttaaaga taagattaga
     2461 agaggcatgc aatcaatcgg aaatggttaa agttttgaag aatcaagcta ctgagtttat
     2521 ttgtagtaaa tataattggg ataaaatcgt acaagaaaca ttaaatttgt ataggggaaa
     2581 tatataatga ttcgaatatt acagtgtgtg aatgatatgc atcgtgcagg gttggagacg
     2641 atgctaatga actattatag gaatatagat cgaactaaaa ttcagtttga ttttttgaca
     2701 catcgtccat ataaaagtga ttatgatgag gaaataataa gtcttggagg aaaggtctat
     2761 tatgcaccga gactttatcc acaaaattat cctaaatatt ttaaatggat ggaacaattt
     2821 tttaaggatc atccagaata tgagattgtt cattctcata ttgatgcgat gagttattta
     2881 cctttaaaag ctgcaaaaaa agctggga
//
LOCUS       DACDBG010000169         2863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDBG010000169 DACDBG010000000
VERSION     DACDBG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.172"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            160..555
                     /locus_tag="IVZ90_002842"
     CDS             160..555
                     /locus_tag="IVZ90_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263821.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            880..1497
                     /locus_tag="IVZ90_002843"
     CDS             880..1497
                     /locus_tag="IVZ90_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8263822.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1538..1837
                     /locus_tag="IVZ90_002844"
     CDS             1538..1837
                     /locus_tag="IVZ90_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263823.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1871..2038
                     /locus_tag="IVZ90_002845"
     CDS             1871..2038
                     /locus_tag="IVZ90_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8263824.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2083..2688)
                     /locus_tag="IVZ90_002846"
     CDS             complement(2083..2688)
                     /locus_tag="IVZ90_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8263825.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1012 a          417 c          524 g          910 t
ORIGIN      
        1 ttggagatga ggggaaagtt gcaaatggat tttctgaaaa aattattcaa tttcaagaaa
       61 atagaagtaa acttaattaa gaatcgttta ctatttaaac agtgtccttt tcctttttaa
      121 atatatacga taaaataagt atatgaattg taaaaggaca tgatgattat ggaagaaata
      181 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
      241 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
      301 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
      361 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg
      421 tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat
      481 attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt
      541 gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg
      601 tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa
      661 tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg
      721 ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt
      781 tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt
      841 tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc
      901 cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga
      961 atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa
     1021 tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat
     1081 agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag
     1141 cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta
     1201 gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa
     1261 gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag
     1321 ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct
     1381 gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga
     1441 aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag
     1501 atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt
     1561 cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc
     1621 tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca
     1681 gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga
     1741 cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat
     1801 tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag
     1861 gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa
     1921 tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca
     1981 aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa
     2041 acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt
     2101 ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg
     2161 aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt
     2221 tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa
     2281 tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag
     2341 cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc
     2401 taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt
     2461 attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga tgacgcaatc
     2521 accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat ctttaccact
     2581 ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca attgtcgatc
     2641 caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa aaaactcctt
     2701 tttaaaagtg atataattta catgtaaaat ttacttgtag ttttactgct atcatagcat
     2761 ttttaaaaat aaattttatg tagacttaaa atttacttca tggagggttt aaatggatga
     2821 aatcaaaagt aacaaaaaag catttggcat ttatttagga gaa
//
LOCUS       DACDBG010000170         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDBG010000170 DACDBG010000000
VERSION     DACDBG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.173"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IVZ90_002847"
     CDS             562..843
                     /locus_tag="IVZ90_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263826.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IVZ90_002848"
     CDS             845..1261
                     /locus_tag="IVZ90_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263827.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IVZ90_002849"
     CDS             complement(1422..1688)
                     /locus_tag="IVZ90_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263828.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IVZ90_002850"
     CDS             complement(1646..2620)
                     /locus_tag="IVZ90_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8263829.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACDBG010000171         2844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDBG010000171 DACDBG010000000
VERSION     DACDBG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.174"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..660)
                     /locus_tag="IVZ90_002851"
     CDS             complement(<1..660)
                     /locus_tag="IVZ90_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263830.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FS"
     gene            complement(1374..1664)
                     /locus_tag="IVZ90_002852"
     CDS             complement(1374..1664)
                     /locus_tag="IVZ90_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263831.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1872..2564)
                     /locus_tag="IVZ90_002853"
     CDS             complement(1872..2564)
                     /locus_tag="IVZ90_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8263832.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          870 a          516 c          395 g         1063 t
ORIGIN      
        1 agagaaaatt aattctgcta agcttattat tgaaaatact gataacttat aattatatcg
       61 tccattgaca atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac
      121 ttgttcttga ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt
      181 tatttcttct cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac
      241 tggccactga tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg
      301 tattaaacag tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac
      361 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
      421 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      481 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      541 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      601 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      661 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      721 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      781 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      841 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      901 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      961 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
     1021 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
     1081 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
     1141 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
     1201 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
     1261 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
     1321 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1381 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1441 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1501 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1561 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1621 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1681 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1741 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1801 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1861 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1921 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1981 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     2041 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     2101 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     2161 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     2221 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     2281 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     2341 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaag cgcttgctct
     2401 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2461 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2521 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2581 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2641 ttacatgtgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2701 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2761 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2821 ttactgtatt tattacaaaa aacg
//
LOCUS       DACDBG010000172         2805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDBG010000172 DACDBG010000000
VERSION     DACDBG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.176"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..461
                     /locus_tag="IVZ90_002854"
     CDS             <1..461
                     /locus_tag="IVZ90_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8263833.1"
                     /translation="TKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERP
                     ATKQEQSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQK
                     PVTSTAPTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            458..1459
                     /locus_tag="IVZ90_002855"
     CDS             458..1459
                     /locus_tag="IVZ90_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8263834.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            1474..2388
                     /locus_tag="IVZ90_002856"
     CDS             1474..2388
                     /locus_tag="IVZ90_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8263835.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            2633..2749
                     /locus_tag="IVZ90_002857"
     CDS             2633..2749
                     /locus_tag="IVZ90_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8263836.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT          940 a          524 c          579 g          762 t
ORIGIN      
        1 aaaccaaagc ggataaagcc agtggacgca agaaacagca ggaacaaaga cggaaaacca
       61 ttgcggagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca gcttcttccg
      121 ttcatgaaag accagccact aagcaagaac aatctcatga tggacagact tcaaggcaaa
      181 caactgtaca ggcttcctat agggaatctc aacaagccaa acaagagcgt ccaacagtta
      241 agtccgattc ttcaagttta aaaacggaac gtcaaagtaa tacagttcaa gaaagaacgg
      301 ttcaaaagcc agtaacttca actgcaccaa cagatagagc ttcacaacgt tcaatcacaa
      361 aagaacgtca gtctactgtt caaagagtac cactacaaaa tacaaaaagt agaccaccaa
      421 tcaaaaccgc caccattaag aaaggcagta agaaaccatg aagctgaaaa ctttagtgat
      481 tggtggttcg ggattattct tgatggtctt ctcactgctt ctgtttgtcg ccattttatt
      541 ttcagatgaa caagatggcg gtttttccaa cattcactat ggcggtgtgg atgtttccgc
      601 agaagtgctg gctcataagc ctatggtaga aaaagttgcc aaagaatatg gaattgaaga
      661 atacgtcaat atacttcttg cgattataca ggtggaatcg ggtggtacag cagaagatgt
      721 tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat
      781 taagcaaggt gtgaagtatt tcagtgagtt attagccagt agcgaaaggc ttggtgtaga
      841 tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttaggat atgtggctaa
      901 tcgtgggaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcgggtga
      961 cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa
     1021 ttacgggaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa cagagtttga
     1081 tgatgataca gtacaagcca tcatggacga agctctgaaa tttgaaggct ggaagtatgt
     1141 ctatggcgga gcttccccga ctacctcttt tgattgtagc ggactgacac aatggacgta
     1201 tggaaaagct ggaatcaact taccacgaac agcacaacag caatatgatg taacccggca
     1261 tatcccatta ttggaagcaa aagccggcga tttggttttc tttcattcta cctataacgc
     1321 tggctcttat attactcata tcgggattta tttaggcgat aaccgtatgt ttcatgcagg
     1381 cgaccctatc ggttatgccg acttaacaag ctcttactgg caacagcatt tagtgggagc
     1441 aggacgaatc aaacaataag aaaggacgat tcaatgatga aattcggaaa aaatcagaat
     1501 acagaaaaac agacaccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcgtaaa
     1561 aaggttgtga ttgtcctatg ggtactttta ggacttagtt tcagctttgc gatattcaag
     1621 cactttacag ccattgatac ccataccatt catgaaacca ccatcataga aaaggaatat
     1681 gtcgatactc atcatgtgga aaattttgta gagaactttg cgaaagtcta ctattcatgg
     1741 gaactaaacg acaagtccat tgataatcgc atagaaaacc tgaaagcata tctgacagag
     1801 gaacttcaag ctctgaatgt cgatacagtt cgtaaagata ttcctgtatc gtcttctgta
     1861 aaaggatttc agatatggac ggtagaagca gatggcgaca atcaatttga tgtaacctac
     1921 agtgtagacc aactcattac agagggggaa aatacaaaga ccgtccactc tgcttatata
     1981 gtgagtgtct atgtagacag tactggaaat atggtcctca ttaagaatcc gaccattact
     2041 agcataccaa agaaatcaga ctataaacca aaagctattg aaagtgaggg tacggttgat
     2101 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
     2161 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
     2221 gagtacatct ttcaagaact ggtaaatcct atttacaatc gtaaggataa tcaagtcacg
     2281 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
     2341 gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca aatattggta
     2401 cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg agattccttt
     2461 acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg
     2521 cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc
     2581 ttttttagga gggcttagtt ttttgcaccc agtttaagaa tacctttatc atgtgattct
     2641 aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag
     2701 tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa tggaggaaaa
     2761 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgc
//
LOCUS       DACDBG010000173         2781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDBG010000173 DACDBG010000000
VERSION     DACDBG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.177"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..618
                     /locus_tag="IVZ90_002858"
     CDS             <1..618
                     /locus_tag="IVZ90_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8263837.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..2134
                     /locus_tag="IVZ90_002859"
     CDS             632..2134
                     /locus_tag="IVZ90_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8263838.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2147..2623
                     /locus_tag="IVZ90_002860"
     CDS             2147..2623
                     /locus_tag="IVZ90_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8263839.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT          897 a          380 c          551 g          953 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
      901 tggtggagta gcttatctag tatatactgc aactattagt gggtttcttg aacttttttc
      961 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     1021 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     1081 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     1141 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     1201 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     1261 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     1321 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     1381 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     1441 gtttggatta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     1501 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     1561 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     1621 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     1681 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     1741 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     1801 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     1861 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     1921 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     1981 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     2041 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     2101 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     2161 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     2221 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     2281 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     2341 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     2401 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     2461 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     2521 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     2581 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     2641 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     2701 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     2761 gtaatttaaa gttatgattt g
//
LOCUS       DACDBG010000174         2738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDBG010000174 DACDBG010000000
VERSION     DACDBG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.178"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IVZ90_002861"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IVZ90_002861"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8263840.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1071 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatat
//
LOCUS       DACDBG010000175         2702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDBG010000175 DACDBG010000000
VERSION     DACDBG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.179"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(731..2071)
                     /gene="glnA"
                     /locus_tag="IVZ90_002862"
     CDS             complement(731..2071)
                     /gene="glnA"
                     /locus_tag="IVZ90_002862"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8263841.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2099..2491)
                     /locus_tag="IVZ90_002863"
     CDS             complement(2099..2491)
                     /locus_tag="IVZ90_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8263842.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          796 a          586 c          445 g          875 t
ORIGIN      
        1 ttcaccctta tttacctcct ttacgtaaaa aacgtagtcc acagttctcg gatttttggc
       61 atatcttttc tattaaataa tatcaaaaac aaaacaattc cactaatttt ttcaaagatg
      121 accttttttt caacaaaagt gccttgtagg gattcattga aggtaacgaa tcacataagt
      181 aaaaaataag gagatgcgac aaaagtcgtg cctccttatt tctaaataaa cggtgttctc
      241 ctgccaactc ctcggtcaca agtcacaata tccatcaaac atgagaagct gtttttgaaa
      301 ttatttcttg tgattcggag ttaaatggta ggctcacaac ctctggataa acggtattca
      361 aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca
      421 aaatttttct taatactcga aactaaacgt ctttttcaaa acctcttggt aaacggtgtt
      481 caaaagcttg catctgcggt aataagttca gccaattcaa aaacaagaaa agctgttttt
      541 gcttggctat tcttattact tgttgcaggg cagctttttc acaacctctc cactaaacgg
      601 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
      661 ttccgttagt ttctctaata ctcaggtcaa aacgctgatt tcacagcctc taattcacgg
      721 tattcctgtt ttaataaagt tctagatatt gttctctttc ccactcggaa acagtttgtc
      781 tgaatgccgc ccattcgaga cgttttgctt caacaaaatt tgagaagata tgttgtccta
      841 atgcgtcaat cattacatta tccttacgca attctttgat agcattatgg atagttgaag
      901 gtaagtcctg gatatgtgca gcttctcttt cttcctcatc cattacataa atgttacggt
      961 caacagctgc tggcggtgtg atctgattac ggataccgtc tagtcctgct tgcaacaata
     1021 ctgccattgc caagtatggg tttgcagatg gatcaactga ccgcaattcc aaacgtgtgg
     1081 ataaaccgcg agattctggt acacgtacta atggcgaacg attacggcca ctccatgcga
     1141 cataaacagg cgcttcataa cctggaacga gacgtttata agaattgact gttggattgc
     1201 aaacggctgt gtaggcacga gcatgtttca gcaaaccacc taagaaatga taagctgttt
     1261 cacttagttc caattcaccg tctttatcat agaaaacatt tccttcatca ttgaacaaag
     1321 acatattgca atgcatacca gaaccattga tcccaaataa cggtttcggc atgaaagtcg
     1381 cgtgaagtcc atgcttgcgt gcaatcgttt tgacaacgag cttgaaggtt tggatattat
     1441 cacaagcatc taccacatct gcatatttaa agtcaatttc atgctgtcct ggcgctactt
     1501 catgatgtga agcttccact tcaaagccta gactttctaa ttccaacacg atgtcacggc
     1561 ggcaattttc acctaaatct gttggtgcaa aatcaaaata accaccttta tcattcaagg
     1621 tcgttgtgat cccaccgtct tcgtctaatt taaataagaa gaattctggt tcaggtccta
     1681 aattgaaaga ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc aaattcccgc
     1741 gaggatcgcc ggcaaatggt gtcccatccg gattatagat atcacaaatt aaacgagcaa
     1801 cttttccatg agcactttcc cacgggaaga tcatccaagt ggagagatct ggatacaaat
     1861 acatatcgct ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca aacatcattt
     1921 tattttctaa tactttgtcg atctggctca ctggaacttc tacatttttg atcgtaccta
     1981 agatgtctgt gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg attcgtttga
     2041 tttcttcacc tgttatctgt ttctttacca tggtatccct cttccttttt taatttattc
     2101 agtcaaagaa ttgtctttgc aatccttgcg actgataatg acctgttttg cttaacccac
     2161 cttgcgccaa aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc aacggcttct
     2221 tgttttttgc ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg atcccagcca
     2281 tattcaatcc ctctgaaaga tagtctttaa tctcaagcaa catatcaata tcattcagcg
     2341 aatacatacg tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt tcttcataat
     2401 agcgaatttg ccgagcagac aaatcagtca gcttcataac tgtaccaatt ggaaatacgg
     2461 acatagatcg gcgcaattcc ttttctccca tcagacgtcc cctttcgttt tataagttgg
     2521 ctaaagtgta ccaattaaaa aaaatattgt caactgcata tgttaggttt tctaacatga
     2581 gatctctatt ttttataaaa taacaaaaaa ataagaacac aaaaaaggta tctagttgat
     2641 tttcgtacca gatacctttt attttttatt atactgtact atcctttatt agtgttttta
     2701 tt
//
LOCUS       DACDBG010000176         2665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDBG010000176 DACDBG010000000
VERSION     DACDBG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.180"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IVZ90_002864"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IVZ90_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8263843.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          752 a          534 c          409 g          970 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2581 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2641 atgcgatgaa gcatgttttc aaaat
//
LOCUS       DACDBG010000177         2533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDBG010000177 DACDBG010000000
VERSION     DACDBG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.183"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..379)
                     /locus_tag="IVZ90_002865"
     CDS             complement(<1..379)
                     /locus_tag="IVZ90_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8263844.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="IVZ90_002866"
     CDS             598..1968
                     /locus_tag="IVZ90_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP8263845.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="IVZ90_002867"
     CDS             1989..2273
                     /locus_tag="IVZ90_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263846.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DACDBG010000178         2420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDBG010000178 DACDBG010000000
VERSION     DACDBG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.184"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(804..1322)
                     /locus_tag="IVZ90_002868"
     CDS             complement(804..1322)
                     /locus_tag="IVZ90_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8263847.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          783 a          443 c          359 g          835 t
ORIGIN      
        1 tgatagtaaa gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact
       61 tgcttctgaa aacttatgaa atgaaagacc ttttacatct tgcagaaatc ggtactggga
      121 ttaaactaag caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt
      181 atttattctg ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat
      241 ttaagatctc ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt
      301 agaataaact gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc
      361 tccaaattat aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca
      421 aagtcagaag atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca
      481 tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa
      541 tctttcccct gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt
      601 tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc
      661 accctctacg gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag
      721 caaagaactt atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca
      781 attgcttagc agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt
      841 ctaaaagtcc caaatggagc aactgtattt acatgaataa atttatatac ttcccataca
      901 gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca
      961 ctcattgaat caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat
     1021 tctttcaatg gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga
     1081 ttccatttaa attgatcaga aggcgtttta acagataatc catttctttc atcattttcc
     1141 cattttagaa ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta
     1201 tccacttcct caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata
     1261 tatttagtat aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc
     1321 attatattac ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac
     1381 gatattttaa tccgttctac aatgcatatc tgatatgcat tgactgtcta actcatatgt
     1441 gaatgattta taaaatatcc caacgtctca tctaaagaaa tgttgatcgt cagcaaggag
     1501 ctaaaaaata cgaatatatt tcttttgtag taaaatcgga ctggtttgca tatcattttc
     1561 ttcgtaaata tcaaaatcat gttaactact atcaattact aataaaaact accactttaa
     1621 gcgttaagaa ctgttaagtt tagttaagtg aggatagttc acaaaatcca ttgttaaact
     1681 tcacgaaagt ggtaaaactc aaaatgcttt atataggatt acagcatcgc tctttcttcg
     1741 attgcgcgtt gggtaaaaaa tattatgaag taaaaattga cgatgacacg ttccttacgg
     1801 ctcatcagat gaaacagcta caaaaacgaa atgatcaact cgaagaaaat ttaattctaa
     1861 aaaagcgatt gccatattca cgcctcgctc gacgaactat taaaggtcaa gatttacctg
     1921 aagatcatcc tttttaggaa attgaagctt tttatgttgc acaatgcatt tataatacta
     1981 caatgatgtt ttcaccagat attcttctag ggtgcggtgt aatgaaacag atacacttgc
     2041 aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat
     2101 ttttttccaa aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa
     2161 taacttcttt ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt
     2221 cgatttttat gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat
     2281 agtatggacg tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt
     2341 ttagtgaact acccccactt agcttggata ggctacaatt aactagacag aaaaattaag
     2401 gtgtgtagac tagaagaaaa
//
LOCUS       DACDBG010000179         2403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDBG010000179 DACDBG010000000
VERSION     DACDBG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.185"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IVZ90_002869"
     CDS             5..1150
                     /locus_tag="IVZ90_002869"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8263848.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVZ90_002870"
     CDS             1321..1779
                     /locus_tag="IVZ90_002870"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8263849.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2403
                     /locus_tag="IVZ90_002871"
     CDS             1938..>2403
                     /locus_tag="IVZ90_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8263850.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESV"
BASE COUNT          801 a          452 c          455 g          695 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttc
//
LOCUS       DACDBG010000180         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDBG010000180 DACDBG010000000
VERSION     DACDBG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.186"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IVZ90_002872"
     CDS             207..2069
                     /locus_tag="IVZ90_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8263851.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IVZ90_002873"
     CDS             2069..>2400
                     /locus_tag="IVZ90_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8263852.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDBG010000181         2387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDBG010000181 DACDBG010000000
VERSION     DACDBG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.187"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..429)
                     /locus_tag="IVZ90_002874"
     CDS             complement(79..429)
                     /locus_tag="IVZ90_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263853.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(422..1747)
                     /locus_tag="IVZ90_002875"
     CDS             complement(422..1747)
                     /locus_tag="IVZ90_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8263854.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(2118..>2387)
                     /locus_tag="IVZ90_002876"
     CDS             complement(2118..>2387)
                     /locus_tag="IVZ90_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263855.1"
                     /translation="MFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQD
                     LELLKTLVDSGTPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          694 a          499 c          359 g          835 t
ORIGIN      
        1 attattggga atcctagctt aaatcataga taccgtaagg gattttattc tttatttaaa
       61 actttgcaac agaaccgttc attctaatcg actccattta ttatcctggt aaaattcgat
      121 atgacgtatt tcgtcgtagt gaattttgtc ttttccaacg tagatcccca gctcatcact
      181 acctaagatc ttgccaacaa tatcttctgg atagtatcct tcagcgtttc gttcttcttt
      241 ttgaatagat actgatctat tttttatgac tgcagcgttc aatacttccg caatctcttc
      301 agtcgtcatt tgttcttttg ctaaattttg actggttcgt tgttcattgt cggcatcaat
      361 ttcttcagta tgatcagata aataaaaccc tgcccacttt aaacgtccac gatcattgta
      421 atcaatcatt tttaattccc ccatttccgc ccgcatgacc accaacaagt gtgcttctag
      481 taatgcttct agcgctttct aacattgaag aagcatgtac aatcgacgta aaaccatatt
      541 tttgccgaat cttgtcaata attttatcta aattttcttc gttaatttgc ttttgtggat
      601 cactaaataa atccagctga agcgaatcgg tgtacacaag cttgctatag gttattccaa
      661 cgtgccttac ttcttgtcct tcataatact tatcaaacaa gaaaagacaa taattctgca
      721 attcctttgt attatctgtg ataggtattt tcatctggtg tgaaaatcct ggtctcgttt
      781 ctaagatcga cgtaccaatg tttaaatgaa cacatcctgt tttacaatta tgctgcctga
      841 tacgaatggc tacttgttcc gccatttctt ttaccaccag ctcgatttca tttcttctag
      901 tataatctcg tggtaacact tgtgaattac cataagattt ttcctttgtc ggttctggcg
      961 ggattgctat atctgttcga tcgattccgt tagcatggaa gtacagctgt aatccaatca
     1021 cacctaatct attcttaatc gtataaggat tccagttagc aaggtctcta atgctcaaaa
     1081 ttcccatttg gttgagcctt tttttcattc gagaaccaat tccccaaaag tccgtcattt
     1141 cgggaatatt ccatactttc tcaggaacgt tttcgtacgt ccattccgca ataaatcctt
     1201 cattatgctt tgcttcattg tctaaggcta atttagccaa aaggggatta tcgccgacgc
     1261 ctaccgtagc aatcaaacct aactcttctt taatacgttc ttggatcatt tgagcgagct
     1321 tcttccttcg ctgacttcgt gtttcttcag tcgtaaaaag attcagtgat tttgtcactt
     1381 ttaaaatcga ttcatcgatc gagtaaatca gtagatcttc atcagccaca tatcttctga
     1441 aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt ggaacaacgt
     1501 gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt gtaataccat
     1561 accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta ttatcagatc
     1621 gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata cattcgacac
     1681 ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct tttgaataat
     1741 ccatcataaa taacactcca ttcttccaca ttatacaaac atatgttcga tgttgtaaag
     1801 aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct gcttcttctt
     1861 atgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa tctaataaaa
     1921 actctcactc taaaagagtg agagactgca aattagagta cggacttttt tggagttccg
     1981 ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgataataag ttatcagtag
     2041 acacttacaa aaaattctca tactattgtc acttttctag ataaatggca aaaattttca
     2101 gcattaaatg acaaaaacta atgagaattt gtctttccat ttttctcctc catttttgcc
     2161 aaataacgat aaatggtggt tcttgagacg ttccaccgtt ccgcaatagt cttaataggt
     2221 gtaccgctat ccactaaagt cttcagtaat tccaaatctt gttcagacaa tttttcagga
     2281 cgaccaccaa accgtcctct tgcacgagca gctgctcgtc ctgctgcgga tcgttccagg
     2341 atcaaattac gctcaaattc tgcaaatgcc gcaaacaaat gaaacat
//
LOCUS       DACDBG010000182         2234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDBG010000182 DACDBG010000000
VERSION     DACDBG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.188"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IVZ90_002877"
     CDS             <1..544
                     /locus_tag="IVZ90_002877"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8263856.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IVZ90_002878"
     CDS             complement(900..1472)
                     /locus_tag="IVZ90_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8263857.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IVZ90_002879"
     CDS             complement(1488..2093)
                     /locus_tag="IVZ90_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8263858.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          339 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ctag
//
LOCUS       DACDBG010000183         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDBG010000183 DACDBG010000000
VERSION     DACDBG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.189"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IVZ90_002880"
     CDS             <1..378
                     /locus_tag="IVZ90_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8263859.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IVZ90_002881"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IVZ90_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8263860.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IVZ90_002882"
     CDS             1859..2026
                     /locus_tag="IVZ90_002882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263861.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IVZ90_002883"
     CDS             2080..>2232
                     /locus_tag="IVZ90_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8263862.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDBG010000184         2163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDBG010000184 DACDBG010000000
VERSION     DACDBG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.190"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            437..1051
                     /locus_tag="IVZ90_002884"
     CDS             437..1051
                     /locus_tag="IVZ90_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8263863.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1075..1425)
                     /locus_tag="IVZ90_002885"
     CDS             complement(1075..1425)
                     /locus_tag="IVZ90_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8263864.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1419..1682)
                     /locus_tag="IVZ90_002886"
     CDS             complement(1419..1682)
                     /locus_tag="IVZ90_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8263865.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          732 a          372 c          314 g          745 t
ORIGIN      
        1 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
       61 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
      121 cattgtctat cacctcgaaa tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga
      181 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
      241 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacaca
      301 tactatttcc tccatcatta gtttttgtct atataaggta taccctaact agacacgacc
      361 gcgacattga aaaatctggt tttctcgtgt atgattaggg tgttttttaa atagttgcag
      421 aaagtaggta aaaattttga gagttgcata catacgtgtc tcatccatcg atcaaaatga
      481 acaacggcag attgaagaaa tgaaaaaatt cggtgcagag agaattttca ttgagaaaca
      541 atctggcgca acgattactc atcgacttgt ttttcaagaa gcactggatt tcgtccgaga
      601 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattacg acgagatcat
      661 cgcttcagtc aattacttga agaagaaaaa tgtgcagctg atcatcacaa gtctaccgat
      721 catggccgaa gcaattggca atcctctcct ggataaattc attaaagatt tgatcattca
      781 aattttggcc atgatcgctg aacaagaacg taccgaatct aaacgacgtc aggcacaagg
      841 cattaagatt gcaaaagcga acggtgttta taaaggacgt cctaagctat acagtgccaa
      901 tgcaaaagat ccccaacgtc gtcttgttta taaaaacatc gttgaggatc taaaaaaagg
      961 tgttgcgatc gctaagattg caaaagacta caatgttacg agacaaaccg tatatagaat
     1021 aaaaaaagac agcatggtca atcacgaata gttgatcatg ctgctgtttc tattttattt
     1081 gcttactttt tgaaaaactg ttctaacttt catcaataac tcctcaacta aactatcatg
     1141 cgctttttcc aaaaaaatac actctctggc ttcataatca attgtcgtta gttgatctaa
     1201 taatactttt cctgttgtct tcatttcata ttctgagttt atatttacat atagcggata
     1261 gtttctagtt gtactagaaa ttggagcaac tgtaacaacg ttactataat ccgcaataat
     1321 atcatgactt aaaacaatat aaggtctttt acctgtctgt tcgtgtccag atcgaggtgc
     1381 tgtattcaat aatagaatat cgccttgtct aggttttctc accatttttc attacctact
     1441 gcttcaccta aattttgaat cgtcacatga tttctttcat cagtgtaatt ttcaaataac
     1501 tgttcaattg ttaattcatt ttctatatta gccgcttcga taattagccg cttctgaccg
     1561 tttacttctt cttgtattat cttcactttg tcttctgtat gaattccaag atattccata
     1621 atatactttg gcaatctaag gccattacta tttccccatt tttttatagg taacatttcc
     1681 atagtagaca cctctttctg tatatacata gtataacata cataagaaaa aacgtatata
     1741 cttaattatc cttttctatt aaattagatt aacattccat gaatccataa actatgttaa
     1801 catttatatg aaaatagtcg aaaggaaatc ataatatgtt taagaaaaca aaatctttag
     1861 aatcaggtaa gaaaaaaatt gatctaaaaa taatctcttc tggatacttt aaagatttca
     1921 tagttattga tacaaaatgg aataaaaagg acatagaaaa tattctaaat agtaggaaca
     1981 attaagtgct atcacttttg tttcttcaaa tgaaaagtga cgtcactttt ttagtgctgg
     2041 tatatcaaat cctgcttttt cttgcgcgat caatagatct aacgcaatac acataatctt
     2101 agattcactt tttccgtact tctgctttag ttgctgcaat tctttttgtc tcttttcatc
     2161 caa
//
LOCUS       DACDBG010000185         2045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDBG010000185 DACDBG010000000
VERSION     DACDBG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.191"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..321
                     /locus_tag="IVZ90_002887"
     CDS             <1..321
                     /locus_tag="IVZ90_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263866.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="IVZ90_002888"
     CDS             735..1553
                     /locus_tag="IVZ90_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8263867.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="IVZ90_002889"
     CDS             complement(1626..1985)
                     /locus_tag="IVZ90_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8263868.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          685 a          285 c          400 g          675 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattc
//
LOCUS       DACDBG010000186         2042 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDBG010000186 DACDBG010000000
VERSION     DACDBG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.192"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..321)
                     /locus_tag="IVZ90_002890"
     CDS             complement(<1..321)
                     /locus_tag="IVZ90_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8263869.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEK"
     gene            complement(388..543)
                     /locus_tag="IVZ90_002891"
     CDS             complement(388..543)
                     /locus_tag="IVZ90_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8263870.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1160..1381
                     /locus_tag="IVZ90_002892"
     CDS             1160..1381
                     /locus_tag="IVZ90_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263871.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1382..1639
                     /locus_tag="IVZ90_002893"
     CDS             1382..1639
                     /locus_tag="IVZ90_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263872.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1775..>2042)
                     /locus_tag="IVZ90_002894"
     CDS             complement(1775..>2042)
                     /locus_tag="IVZ90_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8263873.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          672 a          461 c          312 g          597 t
ORIGIN      
        1 tttttctgga ttatccatat gagcaaaata tcgcaccata ccttttgcat tagcacattt
       61 ttgcgggttt ggagaccgaa gcttttgagt tatctcacgt atttgatcat acgtcttttt
      121 accgctaaac attatcaaaa catgccaatg aggctttttc aattcaccgt caggattcac
      181 atctttatca tgaagtggac tctcaaccca aggaacatgt aactcatcta aaaactccct
      241 ccaattttca ggagccgatt caggatagac tacaaacgtc caattccttg tgcgttcatc
      301 tttagttttt ctactcgtca tgctataatt accttaattg aacaacagtc taacagcaaa
      361 agcagtgcgc caacactaat gcttttgcta ttttttttgc tcattttcct ttccgttagc
      421 attaacccaa attgtaataa gaccagactt actaagaccc atttgtttcg caattttatc
      481 aagattatct gccacttcct tagtaagcgt tacaacgact ctcttttttt cattactgat
      541 cataattcgt atcctccaac gactttttct taaaaaccat agtaacaaaa aagtaactaa
      601 ggtgcaacag tttagatact aattagcgaa taataggcct taaacccttg gcattactga
      661 ttttgtcaaa aaaaaacgct gaaacccttg ggagagtaag gctaaacagc tatcgaccaa
      721 tgaccaatga ccaataaggg gggtgtcgta gtccccccct cctccgccca attcataaac
      781 accaaaacca aaaaacaaac ctcagggctc cgccccgaac cccgacctcc ttctaccgaa
      841 atatatgcag gcgccacgct tactatttcg ttcagctatc ggcttttgca atctaacacg
      901 accgtttcgc taccagtcgc cttacaggct ccattctcgc ttctcttacg tgttttaacg
      961 attgcgccga taactaaacg aaatagacgc taaatcgctt aaaacgcaaa atagaccctt
     1021 tttagagcat tgattttttc tactagtcat ttcgataata taaaattgac cacaacacag
     1081 aaaacttata ttataagctt atgtcgtaag tccgacgtac tcacgtaata tagacggacg
     1141 ttaaatggaa ggagcataaa tgagagtgat aaaatcagaa aaattcactt tggccgaaat
     1201 tatgacgacc agcgaacttg cacaagaact tgattacaac caagcttacg ttttgcgctt
     1261 agcgaaagaa catctgactg aaggaaaaga atatcgctct gccggtagaa gaaactatct
     1321 tttcagccca gaagccctta aaaaactaga aaaagtcata accaccaaag aaggtgagta
     1381 aatgcctaaa gaaacaacta tcaacgttga acaactagaa aaattgctag aatcatattc
     1441 caatgatttt atcgaattga ccggcggaaa agtcgaagaa accaccgacc tctttgacct
     1501 acttctcaca aatattctgg aattaaactc tgaagaacaa aaagagcata agggaaaact
     1561 gatagcacct tcctacagga aggtggctaa gtcggctgtg ccaactggtt ttcttacaaa
     1621 acaaccttat attttttgat atcacaaaaa aatccatttt ggacctattt tcggtcataa
     1681 tatagtacct acttttggtc atagtttcgt ccgtagtttt ggtcatagtg actaagactt
     1741 tcacgcacaa aaaaagagct gaaattaaca gctcttatcc tttatttatt tttgaattta
     1801 cacttccacc atgacgattc gattttatat attgcccgat aatataagct gaattatcac
     1861 aaagaagcgg aaaccaatca tcaaatcttt ctgacattgc atagtcaatt aaatctttga
     1921 tttcagtgat attcttagaa tcaacaaacg acatcatctc tctaatcaac tcatatcgtt
     1981 ctgcactcgt tactgacaaa taactagcaa tttctgcacc accatgagcg ataatatcac
     2041 tt
//
LOCUS       DACDBG010000187         2040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDBG010000187 DACDBG010000000
VERSION     DACDBG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.193"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IVZ90_002895"
     CDS             200..742
                     /locus_tag="IVZ90_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263874.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IVZ90_002896"
     CDS             898..1347
                     /locus_tag="IVZ90_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8263875.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2040)
                     /locus_tag="IVZ90_002897"
     CDS             complement(1377..>2040)
                     /locus_tag="IVZ90_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8263876.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          733 a          322 c          391 g          594 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
//
LOCUS       DACDBG010000188         2022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDBG010000188 DACDBG010000000
VERSION     DACDBG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.194"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..565
                     /locus_tag="IVZ90_002898"
     CDS             47..565
                     /locus_tag="IVZ90_002898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263877.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            573..1613
                     /locus_tag="IVZ90_002899"
     CDS             573..1613
                     /locus_tag="IVZ90_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8263878.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1862..>2022
                     /locus_tag="IVZ90_002900"
     CDS             1862..>2022
                     /locus_tag="IVZ90_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8263879.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          712 a          246 c          414 g          650 t
ORIGIN      
        1 catctttgtt tggggattgt gctgttttaa ttgcactata tgtcatatgt tatacactga
       61 aaaaaacgat aataatattt ttgagagaca cagaatcata ggaacaattg tatgtataac
      121 agttatacca cttctcatat ccataggaat acttttcaaa tttataccaa atgcaatggt
      181 taaccagatg gttcatattg gttctccatt gatggttata gcaagtatat ttttatttta
      241 tgtatttaaa aatttttcat tttacagtaa gtatataaat aaacttgcga gaaccagttt
      301 aggggtatat attttacatg aaaatcatta tttcattgat ggtatgaagt ctagaggctt
      361 aatatgggat gggatatttg gattaggcaa aaggttttta gatagggatt tttggatttg
      421 gtttataatt gcagttgtag ttgtgtgcat aatttgcatg gctatcgatg ttgtaagaga
      481 aaaatttgaa agacagattt ttaaaattaa gagtgtaaag aacctgtgta aacttatgaa
      541 tcatgttata aatatatacc cataattaat aaatgaggag aaaagaagtg aaagttatat
      601 cattcggaga attattattg agacttgcag cacccggtta ttcaaagtta tttcaaagag
      661 atagtttgga gactacattc tgcggagggg aagctaatgt agcggtatcc ttagcaatat
      721 ttggatatca atcagaattt ataacaaagc ttccggattc agatgttgga tatgcggcag
      781 ctaatgcttt gcgttatttt ggagtaaatg ttgataatat tatatttgga gagggacgca
      841 tgggactgta ttatttagaa aaaggagcag ctcagagacc atctaaagta atttatgaca
      901 gaaaattttc tgcaatttcg ttagctgata gtgatgaatt taagtggaag gagatcctaa
      961 acgatgcaaa ttggtttcat tggacaggga taaatccagc attgtctgag aatctctttg
     1021 agacgtcctt agatgcatgt caggctgcca aaaaaagagg tataacaatt tcttgtgatt
     1081 taaattatcg atccaagctt tggacatcag aggaagcaaa agaaaagatg gaacagatac
     1141 taccattggt tgatatttgt atttgtaatg aagaagatgc agaaaaagtg ctaggaatta
     1201 aagctgagca tacaaatatt aatagtggag aattaaataa agatggctat gtgcaggtgg
     1261 ctagggagat tcacaagaag tttggatgta ggtatgtagc tacaactctg cgtagaagtt
     1321 attcggcaag tagaaatggt tggagtgcaa tgttatacga agcagaaaaa gataaagcgt
     1381 atttttccac agaatacaac atacaaattg ttgatcgcgt tggtggtgga gacagctttt
     1441 ctgctggtat aatatatgcg ttatgtgaaa atatgtccgg acaaaaggct attgaatttg
     1501 cgactgcatc tagttgttta aaacatacta tagaaggtga ttttaatcga actacaattg
     1561 aggatgtata taatttaatg aatagcggtg gaaatggtcg cgtgttgcgt tagtcaacat
     1621 ataaagataa atcacagtaa tttttatggt gattatctgt cttagtagtg attagaataa
     1681 cgtgaaaata aattgttgag cgtttatact gttttgaaaa ataaatttaa catattgaaa
     1741 aatacaaata tatacgtata ttataaagcg ccgattgtaa aattaagcta gacaactaaa
     1801 acagtcattt gtgctacact caaaatagtt ccgaccaaag aattataagg agtgagtgca
     1861 aataacctat acctatctta caacggatga acttgtaata atagagtctt atttgaaaat
     1921 taaccaatct gttgctaaaa ctgcgcattg cttgaatcgt tcaagtcaaa cgatccataa
     1981 agtatatctg ttcttcaagc aaagaaaatc agccttagaa ta
//
LOCUS       DACDBG010000189         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDBG010000189 DACDBG010000000
VERSION     DACDBG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.195"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            635..967
                     /locus_tag="IVZ90_002901"
     CDS             635..967
                     /locus_tag="IVZ90_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8263880.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1586..1927
                     /locus_tag="IVZ90_002902"
     CDS             1586..1927
                     /locus_tag="IVZ90_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8263881.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          703 a          272 c          427 g          618 t
ORIGIN      
        1 caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta taataatacg
       61 cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag
      121 gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt
      181 gatgaattga ttttcgttct gcacttttgg atgataaatt tggacaattg aaaatacaaa
      241 aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc
      301 attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat
      361 ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat
      421 acgtgatatt acgatgaatc tatgcctaag cacaatgtta taaaaatgtc cgtgtggctc
      481 tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt
      541 tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt
      601 taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct
      661 caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact
      721 atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa
      781 agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc
      841 tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact
      901 gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat
      961 cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat
     1021 tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga cttgatctat
     1081 caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg taaaaaattg
     1141 gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataataag
     1201 cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt ctagattaat
     1261 catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa ggagtaggtc
     1321 ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac
     1381 ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact agggtttctc
     1441 gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga
     1501 taaaatatga acaagtcctg aataaagtga aaaaattgat ataaaacata gagaaaatga
     1561 aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac
     1621 acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag
     1681 aggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca
     1741 aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga
     1801 atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg
     1861 gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag cggataaaga
     1921 agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt cacgatgatt
     1981 aataaaaagg tagtattacc ttaggcattg agtaaggaat
//
LOCUS       DACDBG010000190         2011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDBG010000190 DACDBG010000000
VERSION     DACDBG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.196"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IVZ90_002903"
     CDS             <1..177
                     /locus_tag="IVZ90_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263882.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IVZ90_002904"
     CDS             190..690
                     /locus_tag="IVZ90_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8263883.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2011
                     /locus_tag="IVZ90_002905"
     CDS             787..>2011
                     /locus_tag="IVZ90_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8263884.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERL"
BASE COUNT          645 a          274 c          456 g          636 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg a
//
LOCUS       DACDBG010000191         1988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDBG010000191 DACDBG010000000
VERSION     DACDBG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.197"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IVZ90_002906"
     CDS             complement(<1..425)
                     /locus_tag="IVZ90_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8263885.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IVZ90_002907"
     CDS             complement(534..1565)
                     /locus_tag="IVZ90_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8263886.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          612 a          327 c          344 g          705 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtag
//
LOCUS       DACDBG010000192         1983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDBG010000192 DACDBG010000000
VERSION     DACDBG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.198"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IVZ90_002908"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IVZ90_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8263887.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IVZ90_002909"
     CDS             852..1040
                     /locus_tag="IVZ90_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8263888.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IVZ90_002910"
     CDS             1053..1610
                     /locus_tag="IVZ90_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8263889.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IVZ90_002911"
     CDS             1642..1884
                     /locus_tag="IVZ90_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8263890.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          306 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttc
//
LOCUS       DACDBG010000193         1964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDBG010000193 DACDBG010000000
VERSION     DACDBG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.199"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..172
                     /locus_tag="IVZ90_002912"
     CDS             <1..172
                     /locus_tag="IVZ90_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8263891.1"
                     /translation="NNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLY
                     HANEVLKIAEEK"
     gene            173..925
                     /locus_tag="IVZ90_002913"
     CDS             173..925
                     /locus_tag="IVZ90_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8263892.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            930..1877
                     /locus_tag="IVZ90_002914"
     CDS             930..1877
                     /locus_tag="IVZ90_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8263893.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          690 a          263 c          390 g          621 t
ORIGIN      
        1 aaataataaa ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga
       61 tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg
      121 gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat
      181 tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata
      241 tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa
      301 acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt
      361 gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga
      421 tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa
      481 tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag tttctaatct
      541 tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact tttcagttgt
      601 ttctgcaaga cgtaatccct attttaatat ggtaaaaaga attgaaaatg aagttactat
      661 agttaataaa tcaagataca catctaggca acagacacct gaagtttttg aaatgaatgc
      721 ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt aattatattt ttgatgggca
      781 atgtgggata ataaaaatga gagattatct aattttagat attgatagtg aagaagattt
      841 taaatggtta gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat
      901 ctataattat ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg
      961 gcttcattgg ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg
     1021 atgacttatc aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg
     1081 agggagatgt cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt
     1141 atatctttca tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa
     1201 cacatcgtgt gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac
     1261 ctgatttaaa acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt
     1321 taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg
     1381 ctgcagaaca atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc
     1441 gttttttcaa tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt
     1501 ctattctcgt agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat
     1561 ttggtgatgg aagccagtct agagactttg tttatataga tgatgtaatc caagcattac
     1621 ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag
     1681 caaccacact tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac
     1741 ttgaatacaa agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga
     1801 tccagtcaat tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga
     1861 aaactgaaat caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt
     1921 caataaggac tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DACDBG010000194         1951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDBG010000194 DACDBG010000000
VERSION     DACDBG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.200"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(247..468)
                     /locus_tag="IVZ90_002915"
     CDS             complement(247..468)
                     /locus_tag="IVZ90_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263894.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="IVZ90_002916"
     CDS             complement(566..811)
                     /locus_tag="IVZ90_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263895.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="IVZ90_002917"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="IVZ90_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          655 a          340 c          286 g          670 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt c
//
LOCUS       DACDBG010000195         1930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDBG010000195 DACDBG010000000
VERSION     DACDBG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.201"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            285..881
                     /locus_tag="IVZ90_002918"
     CDS             285..881
                     /locus_tag="IVZ90_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8263896.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(939..1421)
                     /locus_tag="IVZ90_002919"
     CDS             complement(939..1421)
                     /locus_tag="IVZ90_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263897.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1527..1724)
                     /locus_tag="IVZ90_002920"
     CDS             complement(1527..1724)
                     /locus_tag="IVZ90_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263898.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          668 a          374 c          286 g          602 t
ORIGIN      
        1 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
       61 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
      121 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
      181 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
      241 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
      301 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
      361 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
      421 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
      481 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
      541 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
      601 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
      661 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
      721 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
      781 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
      841 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
      901 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
      961 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     1021 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     1081 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     1141 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     1201 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     1261 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     1321 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     1381 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     1441 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     1501 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     1561 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     1621 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     1681 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     1741 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     1801 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     1861 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     1921 aactaattat
//
LOCUS       DACDBG010000196         1798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDBG010000196 DACDBG010000000
VERSION     DACDBG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.202"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1059
                     /locus_tag="IVZ90_002921"
     rRNA            <1..1059
                     /locus_tag="IVZ90_002921"
                     /product="23S ribosomal RNA"
     gene            1153..1268
                     /gene="rrf"
                     /locus_tag="IVZ90_002922"
     rRNA            1153..1268
                     /gene="rrf"
                     /locus_tag="IVZ90_002922"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1282..1354
                     /locus_tag="IVZ90_002923"
     tRNA            1282..1354
                     /locus_tag="IVZ90_002923"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1315..1317,aa:Val,seq:tac)
     gene            1358..1430
                     /locus_tag="IVZ90_002924"
     tRNA            1358..1430
                     /locus_tag="IVZ90_002924"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Lys,seq:ttt)
     gene            1438..1519
                     /locus_tag="IVZ90_002925"
     tRNA            1438..1519
                     /locus_tag="IVZ90_002925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1472..1474,aa:Leu,seq:tag)
     gene            1537..1609
                     /locus_tag="IVZ90_002926"
     tRNA            1537..1609
                     /locus_tag="IVZ90_002926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1570..1572,aa:Thr,seq:tgt)
     gene            1621..1692
                     /locus_tag="IVZ90_002927"
     tRNA            1621..1692
                     /locus_tag="IVZ90_002927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1653..1655,aa:Gly,seq:gcc)
     gene            1709..1794
                     /locus_tag="IVZ90_002928"
     tRNA            1709..1794
                     /locus_tag="IVZ90_002928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1743..1745,aa:Leu,seq:taa)
BASE COUNT          456 a          398 c          531 g          413 t
ORIGIN      
        1 ctggaaggtt aagaggagtg cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac
       61 ggcggccgta actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc
      121 cgcacgaaag gcgtaacgat ttgggcactg tctcaacgag agactcggtg aaattttagt
      181 acctgtgaag atgcaggtta cccgcgacag gacggaaaga ccccatggag ctttactgta
      241 gtttgatatt gagtgtctgt accgcatgta caggataggt aggagccgta gaaatcggaa
      301 cgctagtttc gatggaggcg ctggtgggat actacccctg cgttatggcc actctaaccc
      361 gcaccactaa tcgtggtggg agacagtgtc agatgggcag tttgactggg gcggtcgcct
      421 cctaaaaggt aacggaggcg cccaaaggtt ccctcagaat ggttggaaat cattcgaaga
      481 gtgtaaaggc agaagggagc ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg
      541 ggcttagtga tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaagctaccc
      601 tggggataac aggcttatct cccccaagag tccacatcga cggggaggtt tggcacctcg
      661 atgtcggctc gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat
      721 taaagcggca cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc
      781 gggcgttgga aatttgagag gagctgtcct tagtacgaga ggaccgggat ggacttaccg
      841 ctggtgtacc agttgttctg ccaagggcat tgctgggtag ctatgtaggg aagggataaa
      901 cgctgaaagc atctaagtgt gaagcccacc tcaagatgag atttcccatt tctttaagaa
      961 agtaagatcc ctgagagatg atcaggtaga taggtcagga gtggaagtac agtgatgtat
     1021 ggagcggact gatactaatc gatcgaggac ttaaccaata ggttaaaaga aaagaactcg
     1081 gaaaaaaact tatgtgactt caaatccagt tttgagtgaa caaaatttac tcaataaaag
     1141 ataacaccac agtgtggtgg cgatagcgag aaggatacac ctgtaaccat gccgaacaca
     1201 gaagttaagc ttcttagcgc cgattgtagt gaggggttgc cccttgtgag agtaggacgt
     1261 cgccacgctt aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag
     1321 cagagggtca gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga
     1381 gcatctgact tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg
     1441 ggtgtggcgg aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt
     1501 tcaagtccct tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag
     1561 catctgattt gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac
     1621 gcggaaatag ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc
     1681 ccgttttccg ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga
     1741 cttaaaatcc tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttc
//
LOCUS       DACDBG010000197         1709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDBG010000197 DACDBG010000000
VERSION     DACDBG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.203"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..989
                     /locus_tag="IVZ90_002929"
     CDS             <1..989
                     /locus_tag="IVZ90_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8263899.1"
                     /translation="PSQISIDVTYKCNFRCKHCYNASGGDNCFMDNELTDDELLELTK
                     DICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFK
                     AGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQ
                     LREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTK
                     IDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGL
                     YKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            1045..1263
                     /locus_tag="IVZ90_002930"
     CDS             1045..1263
                     /locus_tag="IVZ90_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263900.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1334..>1709
                     /locus_tag="IVZ90_002931"
     CDS             1334..>1709
                     /locus_tag="IVZ90_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263901.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEE"
BASE COUNT          655 a          195 c          316 g          543 t
ORIGIN      
        1 gtcctagtca aatatctatt gatgttacat ataaatgtaa ttttagatgt aaacattgtt
       61 acaatgcgag tggtggagac aattgtttta tggataatga gttgactgat gatgaactgt
      121 tagaacttac aaaagatata tgtaatttaa agccatataa tatttgtttc tgtggaggag
      181 aaactttact gagaaaaaaa atagttttat cttgtgccac tttattaaaa gaggcaggta
      241 ttcctaatat tgctattgtt actaatggat cattagtaac tcaggatgtt gcaaaagaat
      301 tattcaaagc aggtattaat cgatttcaag taagtttaga tggaaaaaat gctagtagcc
      361 atgaagcact tagattatat ccaaacagtt ttgaaaaggc aattaatgct atcaaattat
      421 ttagagaatt gtccgaagaa gtggatgttg ctttttgtcc tactagtttt aattacaatc
      481 aattacgaga agtatatcaa ctagtaaata ctcttaaagt gaagtcttta agaattcaac
      541 catttatgct tagtggtagg ggaatgcaaa atagggaaat agtgccaact gtttctcaat
      601 atagaaaact agtttctgat attaactata ttaaaagaaa tgaagattat accaagatag
      661 attggggaga tccaattgat catatcatcc gacacagaac tttattgaaa gatttttcga
      721 attttgtttc tattaggtca gacggaggat taatggcatc accttacttg ccaattcaag
      781 ttggaaatat taaatgtcac agtctttcta aatattggcg agcaggactt tataaagttt
      841 tttctttaga tttagttcaa gatatagcta ctcaagtttc ttcaatacaa gatatgagta
      901 aagaattaaa aaaaggacat attaaatggc ttaatgaaga tatagaaatt gatattctcg
      961 atgaatacaa acatgatatt ttaatttagg tttatcattg gaaagtttag ataaacaaag
     1021 tacttttaga aaggagggaa aaaaatgatt tctgaacctc aaatgaataa tatgtatgaa
     1081 agcccaacta taatgggagc tggtgaagct gcgccaagag cttgttcagc aattatggtg
     1141 tgtgtgttat tagcatctgc aggagtaggt atccaagcag gagcggtgta tacttatgct
     1201 gtagttgcta cagtagctgt agctgcagcc gcagtttgga aaacagtagg attagttggt
     1261 taggaattaa taggagacta actgtctcct attgattcca atgattaagc taaggaggaa
     1321 ttttaagaaa ataatgaaat acaataattt aaaaccgaaa ttacttaatg agaacataga
     1381 atatataaga gaagaaaatt atggatattt aaccatagtt cccaaaaatg gaactgaatt
     1441 gaaagataac gttttcaatc caacagtaag atatatattg gctaactgta ctggaaaaaa
     1501 cagtgtaaaa aaaatagcga atggtttaaa aaagaagttt cctagtttag aatatgaaaa
     1561 attctatcaa gatgttgtag gaactattaa atttttcaaa cactataata ttattgatat
     1621 aggagattat agtatggaag aaaagaaaat atataaaatt gaacaaaatt tagaattaga
     1681 attactaaat gaatcacatt atgaagaaa
//
LOCUS       DACDBG010000198         1651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDBG010000198 DACDBG010000000
VERSION     DACDBG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.204"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1014
                     /locus_tag="IVZ90_002932"
     CDS             <1..1014
                     /locus_tag="IVZ90_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263902.1"
                     /translation="NKLSEKAKENLCKTAFKFVFQLSYGNEEADRDRESTFRLLNALI
                     FDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTKGFFLLTFIATHPELQH
                     DFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNNNEELQNHLNSLVDPIL
                     QQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIY
                     NKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNA
                     VTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDYLRKEINSEKELLNYVH
                     KIK"
     gene            complement(1271..1606)
                     /locus_tag="IVZ90_002933"
     CDS             complement(1271..1606)
                     /locus_tag="IVZ90_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263903.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          622 a          254 c          215 g          560 t
ORIGIN      
        1 aataaactaa gcgaaaaagc caaagaaaac ctatgtaaga cagcctttaa gtttgttttt
       61 caattatctt atggaaatga ggaagcagat agagataggg aaagtacttt cagactactt
      121 aatgccctaa tttttgataa taaagaattc tatttaaata ttctaaaaaa agaaattgca
      181 aagtataggt tcacagctga atcaagagag gaaattaata aatcacttgg tgaaaatcaa
      241 cagatcacgt atacaaaagg gttcttttta cttactttta tagctaccca tccagaatta
      301 caacatgatt tttcagacga gacaattcaa aatttacaaa tatcaataaa tgattttaaa
      361 ccgaaacaac aagtactaac agaaatagaa tattactacc gtttacgttc ccttgcagtt
      421 ctaggaaaca acaatgaaga attacaaaat cacttaaata gtctagttga tcctatactc
      481 cagcaaaaaa tgagagtttc ttcgctacca tcttcaacat tagaaacaat atataatcaa
      541 tatttatatt ttggtgaaaa agagatgttc atagatttta tgatcgaaca tcttaccgat
      601 gctagttcat tcaggttagc agataaggat atggaagtat taccatggat ttacaataaa
      661 ttaaatcagg agcaatttaa caaattacta tacaacctaa atggaaataa tcaattttac
      721 aaaaacagta attttccgcc ttttatttct aatctgttag acttttataa tcagaaattc
      781 caagtgaatt tacgtaaaca ttatagtggg cttctatatc caaatgcagt gactatggat
      841 cagggttttc aattaaagga taaagaatta aaaaaactgt atgaattagc tgaatcgaaa
      901 agtgatatgt atctagacat aatagataat attattaata agtctgaaga ctatttaaga
      961 aaagaaatca attccgagaa agaattgtta aattatgttc acaaaataaa ataatttttt
     1021 ataacacaag atgcactttt atagcgtata tttactttca aattaaacta attagttata
     1081 ttctcacaat agcatttaat aataaatata ctgtcgcttg atttatatat ttccctcttc
     1141 tttctttaga atctctcttt attttgatat aataaaatag aaatcgcacc aaacaatgaa
     1201 atggcggaaa acatttcatt gaaaaaagga ttgaacataa tgttcaatcc ttttttatag
     1261 ttataatcaa ttaacattcg gtgtgtgaaa ttgaatcttc gatgctttca catggaaaat
     1321 tattgattcc ttcttcactc atttcccatg tagtatagaa aggagttaac tgtcctccat
     1381 actcctgaat aataaaatat tttccatcat tgatatggtt acaacagtac actccaatag
     1441 ttggatactc gactactaaa aattggtctg gatcaaactg ttcttttttc aacatttctc
     1501 gtatttgaaa caaatgatcc ttctctaagc aatcaggtaa ttcctttatt ctttgcgtta
     1561 ctaatgaccc ctctatcgct ttccattcat ttaaatattg tttcataaca attcattcct
     1621 tttatgttaa ctatttttcc taggaaattg t
//
LOCUS       DACDBG010000199         1647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDBG010000199 DACDBG010000000
VERSION     DACDBG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.205"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          626 a          196 c          326 g          499 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     1621 tagaatcata ttatcttcaa cataata
//
LOCUS       DACDBG010000200         1633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDBG010000200 DACDBG010000000
VERSION     DACDBG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.206"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IVZ90_002934"
     CDS             144..542
                     /locus_tag="IVZ90_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8263904.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IVZ90_002935"
     CDS             526..1221
                     /locus_tag="IVZ90_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8263905.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          623 a          235 c          321 g          454 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaa
//
LOCUS       DACDBG010000201         1598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDBG010000201 DACDBG010000000
VERSION     DACDBG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.207"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(3..87)
                     /locus_tag="IVZ90_002936"
     tRNA            complement(3..87)
                     /locus_tag="IVZ90_002936"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Leu,seq:caa)
     gene            complement(105..176)
                     /locus_tag="IVZ90_002937"
     tRNA            complement(105..176)
                     /locus_tag="IVZ90_002937"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Gln,seq:ttg)
     gene            complement(186..258)
                     /locus_tag="IVZ90_002938"
     tRNA            complement(186..258)
                     /locus_tag="IVZ90_002938"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:His,seq:gtg)
     gene            complement(320..404)
                     /locus_tag="IVZ90_002939"
     tRNA            complement(320..404)
                     /locus_tag="IVZ90_002939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Leu,seq:caa)
     gene            complement(425..495)
                     /locus_tag="IVZ90_002940"
     tRNA            complement(425..495)
                     /locus_tag="IVZ90_002940"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(461..463),aa:Cys,seq:gca)
     gene            complement(541..612)
                     /locus_tag="IVZ90_002941"
     tRNA            complement(541..612)
                     /locus_tag="IVZ90_002941"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Gln,seq:ttg)
     gene            complement(624..696)
                     /locus_tag="IVZ90_002942"
     tRNA            complement(624..696)
                     /locus_tag="IVZ90_002942"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:His,seq:gtg)
     gene            complement(699..772)
                     /locus_tag="IVZ90_002943"
     tRNA            complement(699..772)
                     /locus_tag="IVZ90_002943"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(738..740),aa:Trp,seq:cca)
     gene            complement(779..859)
                     /locus_tag="IVZ90_002944"
     tRNA            complement(779..859)
                     /locus_tag="IVZ90_002944"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Tyr,seq:gta)
     gene            complement(861..936)
                     /locus_tag="IVZ90_002945"
     tRNA            complement(861..936)
                     /locus_tag="IVZ90_002945"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(901..903),aa:Phe,seq:gaa)
     gene            complement(972..1044)
                     /locus_tag="IVZ90_002946"
     tRNA            complement(972..1044)
                     /locus_tag="IVZ90_002946"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1009..1011),aa:Asp,seq:gtc)
     gene            complement(1054..1126)
                     /locus_tag="IVZ90_002947"
     tRNA            complement(1054..1126)
                     /locus_tag="IVZ90_002947"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1091..1093),aa:Val,seq:tac)
     gene            complement(1142..1213)
                     /locus_tag="IVZ90_002948"
     tRNA            complement(1142..1213)
                     /locus_tag="IVZ90_002948"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Glu,seq:ttc)
     gene            complement(1228..1317)
                     /locus_tag="IVZ90_002949"
     tRNA            complement(1228..1317)
                     /locus_tag="IVZ90_002949"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1281..1283),aa:Ser,seq:gga)
     gene            complement(1357..1430)
                     /locus_tag="IVZ90_002950"
     tRNA            complement(1357..1430)
                     /locus_tag="IVZ90_002950"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1394..1396),aa:Asn,seq:gtt)
     gene            complement(1440..1555)
                     /gene="rrf"
                     /locus_tag="IVZ90_002951"
     rRNA            complement(1440..1555)
                     /gene="rrf"
                     /locus_tag="IVZ90_002951"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          442 a          426 c          364 g          366 t
ORIGIN      
        1 tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag
       61 cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt
      121 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa
      181 taaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa
      241 ccacttcgcc acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag
      301 atgaagacat cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca
      361 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt
      421 actaaggcgg taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca
      481 cttggctata ccgccattat actattatca atctataaaa taaagaataa attatagaaa
      541 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
      601 ggctataccc caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc
      661 cacaatccgg tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga
      721 acccacacta acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg
      781 gaggagagtg gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta
      841 gccacttcgc tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc
      901 ttcaatccgt tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat
      961 aacaagaacg acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca
     1021 tgttaacccc tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga
     1081 ccctctgctt gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa
     1141 atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct
     1201 tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt
     1261 ttggcaccat ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga
     1321 gtgaaagaag ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta
     1381 cgacatcatg attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag
     1441 cgtggcgacg tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct
     1501 taacttctgt gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg
     1561 gtgttatctt ttattgagta aattttgttc actcaaaa
//
LOCUS       DACDBG010000202         1521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDBG010000202 DACDBG010000000
VERSION     DACDBG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.208"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            236..850
                     /locus_tag="IVZ90_002952"
     CDS             236..850
                     /locus_tag="IVZ90_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8263906.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1015..>1521)
                     /locus_tag="IVZ90_002953"
     CDS             complement(1015..>1521)
                     /locus_tag="IVZ90_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263907.1"
                     /translation="EFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRK
                     EIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIE
                     LTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDK
                     EPTNTVGD"
BASE COUNT          521 a          256 c          232 g          512 t
ORIGIN      
        1 tctatcacct cgaaatcaaa actaagaaaa cgttttctta ataaatctta acagataaat
       61 caagttatat actagaaaat tcataaaata tatttttaat gtcataatcg tttaaatttt
      121 cttgattgac atttatttac aaaaaatatt gccatttaaa aagaaaagtg acatatggct
      181 tcctcgtgta tgattagggt gtattttaaa tagttgcaga aagtaggtaa aagttttgag
      241 agttgcatac atacgtgtct catcgatcga tcaaaatgaa caacgccaga ttgaagaaat
      301 gaaaaaattc ggtgcagaga gaattttcat cgagaaacaa tctggcgcaa cgattactca
      361 tcgacctgtt ttccaagaag cattagatta cgtccgagat caagatattt ttattgtgga
      421 agctattgat cgacttggaa gaaattatga cgagatcatc gcttcagtca attacttgaa
      481 gaagaaaaat gtgaagctga tcattactag tttaccgatc atggctgaag caattggcaa
      541 tcctctcctg gataaattca ttaaggatct gatcatccaa attttggcca tgatcgctga
      601 acaagaacgg actgaatcga aacgtcgcca ggcacaagga attaagatcg caaaagcaaa
      661 cggcgtctac aaagggcggc ccaaattata cagtgcagac gcaaaagatc cccaacgtcg
      721 ccttgtttac aaaagcatcg ttgaagatct taaaaatggt gttgcgattg ctaagattgc
      781 taaagactac aatgttacga gacaaaccgt gtatagaata aaaaaagaca gcatggtcaa
      841 tgacaaatag ttgatcatgc tgcttttatt atcttctcat ctttaatttc ctacatataa
      901 ttactatcta taaaattaag tcttactcag aacagataaa gcataaaatt ccaacaaacc
      961 attttttact taactaaata aagagtctgt taatataatc ttcacttatt tacgttaatc
     1021 ccctactgta tttgttggtt ccttatctat attttctaca aaatccttat tatcttgtga
     1081 tttagcttcg tataattctt tgtatgtcga cacattggtt atactaattc ctatagctag
     1141 ttcttgagac tctacttttc tctctagctc ctttgacaaa gtgatttgag atttatttgg
     1201 ttccgttgga gtcaattcta tagattcttc ataaaaatct ctatacataa agctatcatt
     1261 aattttataa aaaaaattaa atgtttttag cactgccata taacattcaa aacattcttc
     1321 tatagttggt ttacttcctt gaggagaatg aaccaattta ttacgttttt ctatgaaatt
     1381 tttaatttcc tttcttattt ctttcggaaa ctcaatatta aagtaccctt taaacatgct
     1441 ccctaataat ttgtctacag ggggagattg taaattctca aatattagag ttaaatcagg
     1501 tttctcttgt tgataaaatt c
//
LOCUS       DACDBG010000203         1472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDBG010000203 DACDBG010000000
VERSION     DACDBG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.209"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..1252
                     /locus_tag="IVZ90_002954"
     CDS             50..1252
                     /locus_tag="IVZ90_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8263908.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
BASE COUNT          529 a          250 c          286 g          407 t
ORIGIN      
        1 aagtaattga agaaattata ggtgttcctc tttcacagga ggaataacta tgaaaacaaa
       61 atacccttgt gtgtatcaag ataaaaatgg tcgcttttct tatctagttg agttaggaac
      121 agatcaagtg actggaaaga ggatccagaa gaaaggaaca aaagattctc taggaaaacg
      181 attctcatca gctcgtgaag cacacaaaga agtcatgcgt ataaaaaatg aatacatggt
      241 tgtaaatgga tttgcgaatt atgatttaac ttacaaagaa tttatggagc agtcttacgt
      301 tccacactac aaagctagcg tacaaaaaag tacttggagt tctcgtgagt gtggattgat
      361 tcagattact gaatttttcg gtgataagaa gcttcgtgaa atcaatgtgc gagattgtga
      421 aaattatcga atttggttat tgaacgatag cggatactca caagcataca gttcacttat
      481 gtacggtatg tttcgtaaat cattagacta tgcggtcacc ctgcaatttc tgaatgagaa
      541 tatttccaaa agaacaaaag caattccaaa aggaaaatca atcgttcctt actggacaaa
      601 agaagaattt gaagaagttt tggcaaaaat ttataaagat gatttctacg aacatatgtg
      661 ttttttaatg atctggctat attacatgac tggtgttcgt gtaagcgaag gattagcact
      721 taattggaat gacgtggact tcaaacgtaa aaagttgagg gttcatcaca ccttggaaat
      781 gacaaatcaa aaagatttta ttcggaaacc ttacacaaaa actgaaagcg gcttgcggat
      841 catttctctg gacgatgata caattgaagt gctaaaaaag tggaaagtta ttcaaaaaaa
      901 tcatggtgta gaacaattta ttcttagcta cactgacctc ccactctatc gttcaactgt
      961 tcaacgaatt attcagcgtt atgccaaact agctaaggta cccctaatac aaggaaaagg
     1021 attacgtcat tctcatgtta gctacctaat caatgagttt aacgcagaca ttctggtcgt
     1081 gtcgcaacgg ttaggccatt ctagccctga aatcacttta aagcactacg ctcatctatg
     1141 gagccgtaat gatgaaggaa ttgcagaaca aatgacagga aacattaaat ttacctgtgc
     1201 aacaaaatct aaagttgcaa actttaatgg aaatcaagca gtaaaaatct aaccctacca
     1261 tttccctacc aaaatgagga ataggctgaa aaagcactga tataatagct tctttacgag
     1321 gctactcata ttgagtggga ataagacgta aatataatat ggatggggaa tgccgataaa
     1381 tcaacgtttt tcaacgtaag cgccccatag aacagtacct tactaacaaa tgcacctcaa
     1441 gatatacttg agctatcaag aagtctggag gt
//
LOCUS       DACDBG010000204         1454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDBG010000204 DACDBG010000000
VERSION     DACDBG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.211"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1410
                     /locus_tag="IVZ90_002955"
     CDS             <1..1410
                     /locus_tag="IVZ90_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8263909.1"
                     /translation="FFTEMWERFSYYGMRALLIYYMYDTVANGGLGLPRATALAIMSI
                     YGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFGVSTLFVSMALIII
                     GTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLIVGTIGQEYNYHLG
                     FSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRNLIIALVVIAAVFG
                     GAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPRVWAYIPLFLAAII
                     FWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFILTPVFVTMWTKLGE
                     RQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSFFIVVIAELCLSPV
                     GLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTESAYFGIIGAVAIVG
                     GILLFTVKEPIKKLMGSIH"
BASE COUNT          396 a          278 c          302 g          478 t
ORIGIN      
        1 ttcttcactg agatgtggga acgatttagt tattacggta tgcgtgcatt acttatctat
       61 tatatgtacg atacagttgc caacggggga cttggattac cacgagcaac agcattagct
      121 atcatgtcga tctacggatc tttgatctac atgtctagta tcgtcggcgg atgggtatct
      181 gaccgagtgt taggagcaaa acgcactgta ttcttcggtg gagttttcat tatgcttggc
      241 cacatcgtct tggctttacc atttggtgta agtacattgt ttgtttcgat ggcattgatt
      301 attatcggaa ccgccttttt gaaaccaaat gtttctggca tggtcggaca tctttattca
      361 aagactgact tgcgaagaga tgcaggattt tcgatttttt acatgggtgt caacttaggg
      421 gcattattag caccactaat cgttggtaca atcggtcaag aatataacta tcatttaggg
      481 ttctctttag ctgctattgg gatgttcttt ggattattac aatacatcat ccaaggacgc
      541 aagacattag acggagtggg tacaacaccg ccgaatcctt tgacaactga agaacgtaaa
      601 ggattcatcc gtaacttgat catcgcactt gttgttatcg ctgcagtatt cggcggggca
      661 caactaactg gtcatctgac aattgacttt tttgtcaata caatcagtgt attggggatt
      721 atcttaccgt tgtattactt tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa
      781 aaaccaagag tatgggctta tatcccatta ttccttgcag caatcatttt ctggtcatta
      841 caagaacaag gttcatctat cttagcttta tttgctagtg aacgtacaca atctacgcta
      901 tttggtgtaa cgatccaagc aagctggtat caatcattga atccaatttt catctttatt
      961 ctgacaccag tattcgtaac gatgtggacg aaacttggtg aacgtcaacc atctacagta
     1021 gtgaaattct ctcttggact tttatttgca ggattatcgt acttgctatt gatgttgccg
     1081 ggtatgcttt atggtacaag tggaaaagta agtccattgt ggttgttggg aagtttcttc
     1141 atcgtggtta ttgctgaatt gtgtctttca ccagttggtt tgtctgtgac aacaaaatta
     1201 gcaccacgag catttgaatc acaaacaatc gctatttggt tcttagctga tgcttcttca
     1261 caagcaatca atgcacaaat tgcacgcttc tacacaccgg gaacagaatc tgcttacttc
     1321 ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc tatttactgt gaaagaacca
     1381 atcaaaaaac taatgggctc tattcattaa tcaatgaaca taaaaacagg aattgcttat
     1441 ttgcagcagt tcct
//
LOCUS       DACDBG010000205         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDBG010000205 DACDBG010000000
VERSION     DACDBG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.212"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IVZ90_002956"
     CDS             complement(<1..625)
                     /locus_tag="IVZ90_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8263910.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IVZ90_002957"
     CDS             complement(784..1050)
                     /locus_tag="IVZ90_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8263911.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IVZ90_002958"
     CDS             complement(1040..1396)
                     /locus_tag="IVZ90_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8263912.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DACDBG010000206         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDBG010000206 DACDBG010000000
VERSION     DACDBG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.213"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IVZ90_002959"
     CDS             105..1406
                     /locus_tag="IVZ90_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8263913.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DACDBG010000207         1434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDBG010000207 DACDBG010000000
VERSION     DACDBG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.214"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IVZ90_002960"
     CDS             <1..1350
                     /locus_tag="IVZ90_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8263914.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          564 a          225 c          311 g          334 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgt
//
LOCUS       DACDBG010000208         1434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDBG010000208 DACDBG010000000
VERSION     DACDBG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.215"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            316..1356
                     /locus_tag="IVZ90_002961"
     CDS             316..1356
                     /locus_tag="IVZ90_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8263915.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          562 a          191 c          226 g          455 t
ORIGIN      
        1 agtatgtttt atatgcataa ggtagttctt aatttatttt ttgttttgac actggtagta
       61 cttaattatc tttaatttta aaactaaatt ttattcatac gtttttctgg tagtatttaa
      121 ttataaataa tatttattta aagtagttct taatgacttt aaaggtagta cataattcat
      181 tttatttctg attttaggta gtatttaatt aaaaaacagt tttaatgcgt ttgcattttt
      241 tatttatctt tgctattttc gaaaagagta ggtaatttta cataaataaa acaacgtgaa
      301 agtagaggat ctacaatgac tgattttaaa ttttttaagg cggatagagt ttacaacgaa
      361 ttattttatc aatttccaaa agtctttatt gtttctgacg aatacaaaaa aatgaaagat
      421 tcaactaaga ttgcctatat gcttttaaaa gcaagattag agatcgcaat cagcaaacgg
      481 caaatcgatg aagaaggtaa tgtttatttt acttatacga caaatgaact atgtagagta
      541 ttaaactgcc aaaaacaaaa agcgatagca atcaaaaaag agttggaatc ctttggttta
      601 ttattacaaa agcagatggg atttaacaaa cagttaggga aaaataatcc taatagacta
      661 tatctagcag aattaaaagt ctcagaaaat gatatctact tactcgaaaa atttgataga
      721 gagaataggg aaaacgttga taaatcagag ggtatgaaaa tcatacccac cctcgacgaa
      781 aaatcagacg ctgaatccct tggggctcaa gagggtatga aaatcatacc gtgccaaaac
      841 gttgataaat cagagggtat gaaaatcata ccagaactta ataataatat attagacact
      901 aatagacaca atatagacac tgaaaaagac cgcctacaag atcaattgtt gttagacaat
      961 tttgagacaa ttatgacaaa cgacagcatt gctacgtttg tccctgaacg agtattaaat
     1021 ttgataaaaa cattttcttc aagttacagt gaagctcaaa aaaccgtcca gactattcat
     1081 aatgcaaaga aaaaagctga aatagaaagt ggtatttcga tagtttttga agaactcgat
     1141 agttattatg tcaatgcaga acaagaatta tacacgacac tgttaaaagc ctatcaaaaa
     1201 ttaaaaaccg aaaaagtcga aaatatccag aacctgattt ttgtctatgt aaaaaattgg
     1261 tttatcgaaa aaccaatagc tgctaaagta tcaagtgaaa aacgtttgaa ttatgaaagc
     1321 tccccaagca ctattacgaa agactggtta gagtgaaact ttactgacaa agtgattgtt
     1381 gcttcatgga gtaacattaa aattaaaaat tttttattat tctaattata ttac
//
LOCUS       DACDBG010000209         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDBG010000209 DACDBG010000000
VERSION     DACDBG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.216"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IVZ90_002962"
     CDS             complement(150..1337)
                     /locus_tag="IVZ90_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8263916.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDBG010000210         1402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDBG010000210 DACDBG010000000
VERSION     DACDBG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.217"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..831)
                     /locus_tag="IVZ90_002963"
     CDS             complement(<1..831)
                     /locus_tag="IVZ90_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8263917.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSIAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSGGAVVGGIAGNIVFEVFLRLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWIWYLICGGILGFLASFLGIGGGPI
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSGFMRFDLSMLFYIIPAAVI
                     GGLWGAKFSRILSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     S"
BASE COUNT          481 a          284 c          253 g          384 t
ORIGIN      
        1 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
       61 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
      121 aaaggtcact ttttttggag acaaaatcct gctaaatttt gctccccaca aacctccaat
      181 aacagcagcg gggataatat aaaacaacat agataaatca aagcgcataa agccacttgt
      241 taaagcaatc gttactaatt tagccagttg agaaaagaaa atcgtagata gagaatacag
      301 cgttgcttct tttattggca aggcaaacat cagcatcagt aatgaaacat tgatgggtcc
      361 accaccgata cctaaaaagc tagctaaaaa gcccaagatc cccccacata taagatacca
      421 aatccatgac gttagatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
      481 caaggtcaat accgtcaaga aaatttggat cagctgcaca tgcttttcat tttcaaacaa
      541 ccgtaaaaaa acttcgaata caatgttacc ggctattcct ccaactacag ctccaccaga
      601 tacccaaccg acaatctgcc aattcagcga tttcccactt gatacttgtc gcactgtcga
      661 aacaatagac atcgtgaata ctgcaaccgt tgagtaaaaa gaaatacctg caatagaatg
      721 ggcaccgata agatcaagaa tcggtttgat caacacgcct ccgcccatgc cggaaatagc
      781 gccaactgta tttgccagaa cgatcaccac aaaataaatc agaccaatca tatcatcttg
      841 tatatccttt ctcttattta tatcttttag cgatttttac tctttgctta tattatacag
      901 taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatg tagtactaaa
      961 cgaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     1021 agctcctcat aattttcgtt tggttgaact tattaccgca gatgcaagct tttgaacacc
     1081 gtttaagaaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa
     1141 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg gcaggagaac
     1201 accgtttaag gaagagactg tgaagtcaac gttttgacct gattattaga gaaacaaatg
     1261 gcaaaatagt tcttcatatt tcgccgtttt tcgagcgaat gccgaggggc agctgattga
     1321 acatcgttta tttggaaaaa gggattgtga caagacgttt gtcacaatcc ctttttttat
     1381 atcttattaa ttcattcttt tg
//
LOCUS       DACDBG010000211         1379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDBG010000211 DACDBG010000000
VERSION     DACDBG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.218"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            192..446
                     /locus_tag="IVZ90_002964"
     CDS             192..446
                     /locus_tag="IVZ90_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8263918.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="IVZ90_002965"
     CDS             447..764
                     /locus_tag="IVZ90_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8263919.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          495 a          197 c          267 g          420 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaagaca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccttagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacttccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattaac
//
LOCUS       DACDBG010000212         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDBG010000212 DACDBG010000000
VERSION     DACDBG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.219"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..>1372
                     /locus_tag="IVZ90_002966"
     CDS             122..>1372
                     /locus_tag="IVZ90_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8263920.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDBG010000213         1315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDBG010000213 DACDBG010000000
VERSION     DACDBG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.220"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IVZ90_002967"
     CDS             <1..848
                     /locus_tag="IVZ90_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8263921.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          392 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaatt
//
LOCUS       DACDBG010000214         1304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDBG010000214 DACDBG010000000
VERSION     DACDBG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.221"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..>1304
                     /locus_tag="IVZ90_002968"
     CDS             22..>1304
                     /locus_tag="IVZ90_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8263922.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          406 a          284 c          261 g          353 t
ORIGIN      
        1 cattcaaagg agtgtttcaa gatgtcctat tcagagttta ccaaagagtt actagatatt
       61 ctagacctaa atcttacctt tcatgaagat gcctttcgca aagagcgcat taatgacgaa
      121 acatgttttg tttttgatgg cacattgacc tatcaaccag aggaatgctt ccattgccac
      181 tatcaaaata agcaaacgat cattaaatgg ggctggaaaa aagtatccat cttattgaat
      241 gatgtcagta actacaagac cattgtgcgg attaacaaac agcgcttcaa atgtaaacac
      301 tgtggaaaga cttttttagc agaagattca gttagcgatc gtcgctgctc cattgcccgc
      361 agagtcaaac aagccattct tgagctatta agtgagccta tttctatgtc tttgattgca
      421 cgtatgaagc acatttctcc gaccactgtc attcgaatac ttcgcagcct tcggcctaaa
      481 accgtttctt tgaaccagcc gttaccagaa gtggtgtgtt tcgatgaatt caaatcggtg
      541 aaaaacgtct cgggagccat gagctttgtc atgatggacg gcaagaccca tcaactgatt
      601 gatattgtgg aaaatcggca actcaatcct ttacgcgact atttcttgcg ctaccctaga
      661 aaagtccgtg aacaagtacg tctagtggtt tcagatttct atacacccta tcgcacattg
      721 gtgaaagagt gttttccgaa tgcccgaatc gtcgccgatc gctttcatat tagccaacac
      781 atcggtcggg cctttaccaa ccaccgaatt caagtaatga agacctttaa aaaaggggat
      841 cgtcggtcga agcacttaaa gaaatactgg agactacttc aaaagaatgc gtgggaattg
      901 aatggtcagc atcgctactg gcgtccgtca tttcgagatc atctgacaga agcggagata
      961 gtcgatcggc tattatatta tgatgactcc ttaaagcgag gctacgaagt ctaccaggtt
     1021 tttctttccg ctatcagaag acaagatgtt cctgaattcg tcgcgctcct aaaagaagac
     1081 tacaaggagt tgccagagca ctatcaacca gtgttcacga ccttcaagaa atatcgaaca
     1141 gaaatcaaac gagcgttgcg agttccctat tccaacggcc ctatagaatg tttgaacaac
     1201 catatcaaag tactaaaacg aattgcctat ggctttagaa actttcaaaa ctaccgagaa
     1261 cgaatctttt tatatcgagg aaaatatttc aagaaaacta agaa
//
LOCUS       DACDBG010000215         1303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDBG010000215 DACDBG010000000
VERSION     DACDBG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.222"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..634)
                     /locus_tag="IVZ90_002969"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="IVZ90_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          409 a          210 c          216 g          468 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tatgtaatat aaaaaaacat gaggtggtat atgaatttga
     1141 aaaaaatttt ttctgtaaaa atatttatag tttatgcatt tgttagttta ttatatttat
     1201 ttccagcatt taaaaacatg gaaatatttg gcttttatga tacggcattt cacattaatc
     1261 gtgcgttaag tttagaatct atttttagct cccctattaa ttt
//
LOCUS       DACDBG010000216         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDBG010000216 DACDBG010000000
VERSION     DACDBG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.223"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(525..1028)
                     /locus_tag="IVZ90_002970"
     CDS             complement(525..1028)
                     /locus_tag="IVZ90_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8263923.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT          494 a          190 c          216 g          388 t
ORIGIN      
        1 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaa
//
LOCUS       DACDBG010000217         1253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDBG010000217 DACDBG010000000
VERSION     DACDBG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.224"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            90..1247
                     /locus_tag="IVZ90_002971"
     CDS             90..1247
                     /locus_tag="IVZ90_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263924.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT          468 a          186 c          228 g          371 t
ORIGIN      
        1 gagggaacga ttggttatat tttactggat tcactttaca aaggtactta tcaatcctta
       61 gaaaatctta gtcaaaaaat tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac
      121 ttaatactat tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt aatccgatag
      181 aaggagaaga ataccgtatc cgatactttt ttcacttgat gtattggcag tcattcgacg
      241 aaaatcattc tgatatatta tcaagtataa aaaataaacg tcaactatta gaaaaatttg
      301 cgaaacatgc gccatatacg cgtagtatag atagaaagaa atggatcagc ctactaagaa
      361 tttcgttgca acgaatgaaa caaggccact cgattacgta tgtcccagaa gagatgaaca
      421 aatttgtgca tccattgata acaaaagaag agtttacgca ttacatacta gagccttttt
      481 ttgaagcaaa cttctggtcg atgaatcaca tcactccaac agagcaagct tacctttatt
      541 ttatgtttgg tgtaatgaac acctatttgg aagaagattt gttggatact ccagataaag
      601 tacaagaaag ttggggctgg attgaatcgt ctattgtaga aaattttgaa aaaaacttca
      661 agatttgttt tacaacttca gaaaaaaatt atctatatgt caatttggca atgatccatt
      721 tgtattcaag ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag
      781 acagagattt tcagcatata tttccagtga tgtatccaat catgcaacag ttttttagtg
      841 gggtagctcg cagaattcca gagcttaata cttattatga aaagaacaga cggttgattt
      901 ttcaatactg tatgttagtc agggatattt ttataaaaca cgaacaacct gtcatattct
      961 atattcaatc aaagtatggt aaaacacagg aattatgggc aaagaaaagg atattatcta
     1021 tgactgaacg cccaataaaa tatagtgcat ccgctgatca gttacctgat attgtcatta
     1081 gcgattatcc tatcgacaaa aaggcatatg atcaaacgaa tacacagata ttttactgga
     1141 atggacaacc tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata aaaatcagga
     1201 atgagaaaaa tagatccata ttggaaaaat tggatttcgt taattaagaa aag
//
LOCUS       DACDBG010000218         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDBG010000218 DACDBG010000000
VERSION     DACDBG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.225"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IVZ90_002972"
     CDS             <1..1244
                     /locus_tag="IVZ90_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8263925.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDBG010000219         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDBG010000219 DACDBG010000000
VERSION     DACDBG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.226"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1244
                     /locus_tag="IVZ90_002973"
     CDS             82..>1244
                     /locus_tag="IVZ90_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8263926.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYP"
BASE COUNT          413 a          245 c          239 g          347 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atcc
//
LOCUS       DACDBG010000220         1225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDBG010000220 DACDBG010000000
VERSION     DACDBG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.227"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(227..1087)
                     /locus_tag="IVZ90_002974"
     CDS             complement(227..1087)
                     /locus_tag="IVZ90_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8263927.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          364 a          233 c          179 g          449 t
ORIGIN      
        1 ttttgtgtgt tgcttatttc cctctaacta tcgttttcta tttgatggtt aacctactta
       61 aaataaatac tgcaacgaca aacggagatc aagcatgctt tgtaacgtat tttacgataa
      121 ttacgacttt attttattac tctatcttgc tgccataccc gtctatccct aatcatcttt
      181 ggtgaatttt ttcaaagtta gcataacaaa aataagtatt ttttccttag ttaatcaata
      241 gtatcatttt tttcatatga tttaattcat caattaattt tttgttttta ttaattttag
      301 caaaagctag tgcatcttga atcaattcga gcacttctgc cttcgactta tttttagcct
      361 gtgcgttctt cgctaattgg gcagcagctc gtgctaagta ataagtcaaa tggttatccg
      421 aacaaatatc gattgcgtaa tttagtagtg catcacttgt ttccaaatca ccgtcttgcg
      481 caaaaaaaac accacaatga aagacgatat ttaacacccg ccagatatct tctgttgtac
      541 gaatcggata cgtataaatt ttttcaaaca ctcgattgaa ataaacttct gctttttcca
      601 actctttttc tctagcatag accattccaa tacctgtata tgccaataaa cgaaacaacg
      661 tgttggctgt ttctgccatc aacaactgat caaaatggaa caacacatcc gtaattggtg
      721 cattcgtaaa aatcatttca aatcctttta gatagtagta acgtgcaatc agctcagact
      781 cgtcaattac cttgggatca atggctgcta aaatctgtgt caattgttca tattcactcg
      841 taatcagtga aaactctgct ttttctagct gttcatcaag ttgtgtattg atcacgttca
      901 tcgaagaaaa aagttcaact aacggaagat tgattcgatt acataatttg attaaaattt
      961 ttaacgaagg gacttgacca ttgttctcaa atttacttaa tgttgcttgt gtacaaattc
     1021 cttcagacac ttctttttga gataagttgg cagcctttct tgccgtaata aatttttcaa
     1081 tgttcattat tttctctcca atcagacctc acacgaaaat catttctgtc tcgattttag
     1141 catgttttct ttgggaatgc tttaggttct gttgcaaagt tttaaataaa gaataaagtc
     1201 ccttacggta tctatgattt aagct
//
LOCUS       DACDBG010000221         1209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDBG010000221 DACDBG010000000
VERSION     DACDBG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.228"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..>1209
                     /locus_tag="IVZ90_002975"
     CDS             83..>1209
                     /locus_tag="IVZ90_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8263928.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVH"
BASE COUNT          437 a          182 c          200 g          390 t
ORIGIN      
        1 agatatttag ggtagaaaaa aagaaagtcc attctgtaaa attaaagtta ccacaaccga
       61 aattttatag gaggactttc tcatgcccaa ttttactaca gaaattatgg aaacactaat
      121 caataaaggt gatttagatg aattatttcg atgtcattta gaattagctg ttaattcatt
      181 attgcaggct gaattaacag cttttcttga ttatgaaaaa tatgatcgtg ctgggttcaa
      241 ttcaggtaat tcccgtaatg ggaattattc acgttcattc agaacagagt atggagaatt
      301 aaatttggta attcctagag atagaaatgg taagttttct caacaaactt tacctgctta
      361 taaaagaacc aatgactctt tagaaaccac tattattcag ctatttcaaa aaggaattac
      421 tatgtctgaa atctctgaat tgattgaaaa aatgtacggg catcactata cgccacaaac
      481 catttcaaat atgactaaaa tagtttctga agatattatt gcctttaaag aaaggtcctt
      541 agaatccaga tactctgtca tttttatgga tgctactcac attcctttaa aaagacaaac
      601 tgtttcaaaa gaagcagttt atattgtaat tggcattcga ttagatggaa ccaaagaggt
      661 tcttgggttt agtcttgctc caactgaatc tgcttatgtt tggaaagaga ttcttcaaga
      721 cctaaaagac cgtggtttaa aagaggtttt attagtcgta actgatggtt taagtggcat
      781 taacgatagt attcatagtg tttatccaaa tgctcaattt caacaatgtt gtgtccatat
      841 atctagaaat atcgctcata aggttcgtgt tagtgatcga caagaaatct gtagcgattt
      901 caaattagtt tatcaagcaa gttcaaaaga agaagcaaat aatcaaataa ggtttatgat
      961 agataaatgg aaaaagcagt atccacgagt ggttaaattg ctcatgaatc ctgctatatt
     1021 aaccttttat aatttccctc cgtcaatcag aagaactatt tattcaacta atttgattga
     1081 aggttttaac aaacaattaa agaaatatac caaaagaaaa gagcaatttc ctaatgaaga
     1141 atctctggag agatttctag tatctcaatt caacaactat aatcaaaagt ttctatgccg
     1201 agttcataa
//
LOCUS       DACDBG010000222         1197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDBG010000222 DACDBG010000000
VERSION     DACDBG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.230"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IVZ90_002976"
     CDS             <1..153
                     /locus_tag="IVZ90_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8263929.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IVZ90_002977"
     CDS             159..575
                     /locus_tag="IVZ90_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263930.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IVZ90_002978"
     CDS             681..944
                     /locus_tag="IVZ90_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8263931.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1197
                     /locus_tag="IVZ90_002979"
     CDS             944..>1197
                     /locus_tag="IVZ90_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8263932.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          455 a          154 c          244 g          344 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DACDBG010000223         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDBG010000223 DACDBG010000000
VERSION     DACDBG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.232"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IVZ90_002980"
     CDS             <1..1076
                     /locus_tag="IVZ90_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8263933.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDBG010000224         1179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDBG010000224 DACDBG010000000
VERSION     DACDBG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.233"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            238..471
                     /locus_tag="IVZ90_002981"
     CDS             238..471
                     /locus_tag="IVZ90_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8263934.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            468..875
                     /locus_tag="IVZ90_002982"
     CDS             468..875
                     /locus_tag="IVZ90_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8263935.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          450 a          153 c          190 g          386 t
ORIGIN      
        1 tattaatttg tttcaaaaaa agcatataaa caggtatacg tttaataatt taaaataaat
       61 ggtatacata aatagggaag gaaatagaag ggagcaatct aaattttttg aattttctta
      121 attaattata tagaaagaac aatctttcta tagagttaga catgagaggg actatgcttg
      181 atttaaaatc aacaagcgta tatactgtat atacaaagaa aaaggaggga gaagactatg
      241 gcaattaaag aaagaaaatt tagggaagtg gggaactcag tagtattaac tatttcaaag
      301 gagtttctta aagaaaatgg gttaaatccc ggagatacgg tttttttaga tgaagaaaaa
      361 cttcgagcgg caattacaaa aaaacaagcc ttatctgatg aaattgattt agcaatatct
      421 caagcattaa ctgaatatga tactactttt agagagcttg tcgataaatg agtattcatt
      481 acctagaagc caaagatatt ataaaaatga atgtaatcca aataaaaaaa tactctccta
      541 atgaacccct tggtgtaaag gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag
      601 cctcagcttt tggtgaagag gtttatcctt cgatttatga aaaagcagct atcctactta
      661 tacaattgat aaagaaacat ccttttcaca atgcaaataa acgtactgct ttcttagcta
      721 cttttgtctt tttgaaaatc aatggacagc ttttagctat tgatcaaaaa gatgctgtaa
      781 atttagcggt atatattgct acgtatgata aggatttcga tcaactaaaa tatgaagtga
      841 tcaacactat aaagacacat acgcagccca tttaaggagt tactgtatgt gtctttatag
      901 ggttattagc tgctatagcg aaataattga agtaaattac aaatagctat aattcaaatc
      961 caactatata gaaaacgtaa attttctata tagttggatt aagataacga ctatttattt
     1021 tatcatattt ttactatacc ctatactttt acttggtata tttatgaata atcgcaaatt
     1081 tacccctata ctactagttt gtaagttaga ttaataaaaa tactaaagtt atttgttctt
     1141 ttattaaata cacctacaac ttataaattg atggtattt
//
LOCUS       DACDBG010000225         1170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDBG010000225 DACDBG010000000
VERSION     DACDBG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.234"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IVZ90_002983"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IVZ90_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IVZ90_002984"
     CDS             complement(545..739)
                     /locus_tag="IVZ90_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263936.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          416 a          193 c          164 g          397 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta
//
LOCUS       DACDBG010000226         1170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDBG010000226 DACDBG010000000
VERSION     DACDBG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.235"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..833)
                     /locus_tag="IVZ90_002985"
     CDS             complement(<1..833)
                     /locus_tag="IVZ90_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8263937.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RS"
     gene            complement(844..1050)
                     /locus_tag="IVZ90_002986"
     CDS             complement(844..1050)
                     /locus_tag="IVZ90_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8263938.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          320 a          254 c          194 g          402 t
ORIGIN      
        1 gatctataaa ttccatcagc tcttactcga gctaattctt tcccagtatt gtaatccata
       61 atgaagatat actcgtattt atagttagca atgtgccatc cagctacatg caaatttgca
      121 ttttcgattt ctccaaactg atcaatgtgg gcgtaattcg ttccatctgt cagtgtagga
      181 ttagctgcac cagctcgtgt tggatcaatg acaggcttgt tttcagaagt tgttggattt
      241 tcatcggtaa atccatgagc taaatcatat gctaattttt ctttacttac gcccatttca
      301 gaaagataac cgtaaggatc tgtatgatcg ccccagatgt tttgtgttac ccataaatgc
      361 gatttgattc ccggttggtt ataaggagtg tccaacgtta atggaatacc atatttcatt
      421 gctgaatctc tagccaattc aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca
      481 tgtgtgtgtt gtaactcaat ctgcacagga ctgttggcat tagcatacga accagcaccg
      541 tactgtacat aaccaggttg accgacttga taaacaattc cgccgtctcc cacaatataa
      601 gcagtataag cgctagtcca tgaacgttgc atatactgcg cttcattgcg tcctgttgct
      661 gtttcattag ccgtttcatg cagtaaaata tactgattat ttgctacttg tgagctacct
      721 tcatttgcgc ccaaattaaa ttcattgttg atagtatagg caaatccgtt aattggcaat
      781 aaaaaaagag ccgttaacag gctcatcgca gtaatagtaa ttttcttttt catatctatc
      841 ctcctattta tctgatgaaa ttttcttata cgttcgattt gatacaccta aaatactccc
      901 tagaaatgct ccaaaacctg taataatcat caccgttata tctgtgtacc gccagttaac
      961 accttttccg acaagaccga caaaagtcgc taaagcgggg ataactacta tagcgatcca
     1021 tttcaaaata tcaaaggttc tattgttcat actatccacc tcccttcatt ggttgggcta
     1081 ctttcttttt caatcgcttg ttttcttcta tcaatatttc tctttctgaa agcaaggcat
     1141 caatttgccc gtccagttgt tcaattttat
//
LOCUS       DACDBG010000227         1163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDBG010000227 DACDBG010000000
VERSION     DACDBG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.236"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..548)
                     /gene="dfrG"
                     /locus_tag="IVZ90_002987"
     CDS             complement(51..548)
                     /gene="dfrG"
                     /locus_tag="IVZ90_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8263939.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            778..>1163
                     /locus_tag="IVZ90_002988"
     CDS             778..>1163
                     /locus_tag="IVZ90_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8263940.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT          380 a          213 c          147 g          423 t
ORIGIN      
        1 ccaagctctt gtgctttatc tttactgttc ttcttcaatt aacgcacccg ttaactcaat
       61 aagttttttc tttcatatac atgaaaatag tagttatacg gatttttatc attctttatc
      121 cctttttggg caaatacctc attccattcc tcataattca cttctggaaa aaaagtatct
      181 ccttcgaatt catgatgtat ttttgtgatg tacattttct caacataagg gaaaaacaaa
      241 ttataaatct gttctcctcc gaaaataaaa atttcttctt cgtttttaca taactcaaaa
      301 acatcttcta ttgaatgaac aatttcacaa ccattaaagg taaacccctt atctctcgtc
      361 agaataatat ttcttctgtc aggtaaggct cttccgattg attcaaggtt cttcctacct
      421 aatattatcg gatgtccctt tgtagtattt ttaacatatt cccagtcctt gggaatcctc
      481 caaggaatgt cattctcttt gccaatcact ctattcttat ccatcgcagc aatcaaagaa
      541 actttcattt ataacacagc ctttttaact aatttaccat ctaatttctt atttctatca
      601 aaaaaccttt tctcattatc ctgcccgtta gttgaataag caattttttc tataagttat
      661 attatcataa taccacaaaa tcacaaattt tcattcaatt atcattttca attgcatttt
      721 ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat gaaaaaagta gtccattttg
      781 aagtacattt tgtcattttg tactccaaaa ctgaactact tacttgcata tatacgcctt
      841 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      901 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
      961 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1021 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1081 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1141 agtgatccgc ttctacgata tta
//
LOCUS       DACDBG010000228         1122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDBG010000228 DACDBG010000000
VERSION     DACDBG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.237"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..>1122)
                     /locus_tag="IVZ90_002989"
     CDS             complement(26..>1122)
                     /locus_tag="IVZ90_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8263941.1"
                     /translation="KQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGG
                     NDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITE
                     LMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLF
                     STSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTL
                     CFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLI
                     HLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDT
                     THNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          360 a          163 c          201 g          398 t
ORIGIN      
        1 ataagaggat gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt
       61 tcatatacag ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg
      121 tatccagata gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca
      181 aatcagcatg ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca
      241 aatggctttg aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta
      301 actgctcttt cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga
      361 tttggtttct ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt
      421 cctgtataaa agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac
      481 tactgctgga ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat
      541 aaattggtat tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca
      601 attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct
      661 gtttttgtcg aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg
      721 ctctctctgt ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg
      781 aataatcatg tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg
      841 gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt
      901 tttcttgttt tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta
      961 attcaagtga ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa
     1021 gatagagacg gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga
     1081 tccgtaaatg gaattttttt aaggaatgat tgagctgttt tg
//
LOCUS       DACDBG010000229         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDBG010000229 DACDBG010000000
VERSION     DACDBG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.238"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IVZ90_002990"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IVZ90_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8263942.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DACDBG010000230         1097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDBG010000230 DACDBG010000000
VERSION     DACDBG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.239"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IVZ90_002991"
     CDS             complement(<1..626)
                     /locus_tag="IVZ90_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8263943.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1097
                     /locus_tag="IVZ90_002992"
     CDS             914..>1097
                     /locus_tag="IVZ90_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8263944.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEV"
BASE COUNT          352 a          182 c          212 g          351 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgt
//
LOCUS       DACDBG010000231         1084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDBG010000231 DACDBG010000000
VERSION     DACDBG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.240"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1084
                     /locus_tag="IVZ90_002993"
     CDS             <1..>1084
                     /locus_tag="IVZ90_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8263945.1"
                     /translation="KKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETIV
                     RFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVS
                     LSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICA
                     DGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVI
                     DRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTW
                     KSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPE
                     TLDDGFREKLQNLLTYEEGITNAMIYP"
BASE COUNT          347 a          216 c          214 g          307 t
ORIGIN      
        1 agaagaaaaa gacgttgatc gtcgatgctg ttttctcacc tgctccccat acctgtagaa
       61 attgtggttc tactgtggta gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga
      121 aggaaacgat tgtccgcttt gaacaataca atcacatgcc tttggttatg cgcttaaaaa
      181 agcagcgcta tacctgtaaa aactgtcgaa cccattggac ggctcaaagt tattttgttc
      241 aacccagaca ttcaatcgcg aatcatgtca gatataaaat tgcttcttta ctgactgaaa
      301 aagtatcttt atcttttatt gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta
      361 cgttgaaaga gtttaaaagc tacttaccaa agcaatctaa gaggattctc cccagagtat
      421 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      481 cagatggcga aacaggacaa ttaattgatg tcttgcctac gcgtaaattg cctcgattaa
      541 caagctattt cttaggctgc gctaatcctg aagaagtaaa atttttagtc acagacatga
      601 acgccgccta cttccagctt accaaacgta ttctgccaaa tgcgaaagta gtgattgatc
      661 ggtttcatat tatcaaacac atgaatcaag cctttaatga gttgcgtatc cgtgaaatga
      721 atgaacttcg taaagtagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      781 ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg aaaagttttc
      841 gagcaccaaa atacccattt cttacggaag cgatgatgat tgatcgattg cttggctttt
      901 ctacgtccct gaaggaggcg tatccctatt ttcatgaatt agttgaagcc tttcgagaca
      961 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1021 ttcgggaaaa gctccaaaac cttctgacct atgaagaagg catcaccaac gcgatgatct
     1081 atcc
//
LOCUS       DACDBG010000232         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDBG010000232 DACDBG010000000
VERSION     DACDBG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.241"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IVZ90_002994"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IVZ90_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8263946.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          315 a          219 c          181 g          355 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa
//
LOCUS       DACDBG010000233         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDBG010000233 DACDBG010000000
VERSION     DACDBG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.242"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1064
                     /locus_tag="IVZ90_002995"
     rRNA            <1..>1064
                     /locus_tag="IVZ90_002995"
                     /product="23S ribosomal RNA"
BASE COUNT          327 a          211 c          309 g          217 t
ORIGIN      
        1 cttggcacta ggagccgatg aaggacggga ctaacaccga tatgctttgg ggagctgtac
       61 gtaagctatg atccagagat ttccgaatgg gggaacccaa catctttaat aggatgttac
      121 gattgtgtga atacatagca cattcgaggt agacgcagag aactgaaaca tctaagtacc
      181 tgcaggaaga gaaagaaaat tcgattccct gagtagcggc gagcgaaacg ggaaaagccc
      241 aaaccagcaa gcttgcttgt tggggttgta ggactccaat atggtagttc tttcagatag
      301 tcgaatgact tggaaaagtc agtcaaagag ggtaaaaacc ccgtagacga aatgtggaag
      361 acacctagga ggatcctgag tacggcggaa cacgagaaat tccgtcggaa tccgggagga
      421 ccatctccca aggctaaata ctccctagtg accgatagtg aaccagtacc gtgagggaaa
      481 ggtgaaaagc accccggaag gggagtgaaa tagaacctga aaccgtgtgc ctacaacaag
      541 tcaaagcccg ttaatgggtg atggcgtgcc ttttgtagaa tgaaccggcg agttacgatt
      601 gcatgcgagg ttaagttgaa gagacggagc cgcagcgaaa gcgagtctga atagggcgtt
      661 tgagtatgta gtcgtagacc cgaaaccatg tgatctaccc atgtccaggt tgaaggtgcg
      721 gtaaaacgca ctggaggacc gaacccacgt acgttgaaaa gtgcggggat gaggtgtggg
      781 tagcggagaa attccaaacg aacttggaga tagctggttc tctccgaaat agctttaggg
      841 ctagcctcgg aattgagaat gatggaggta gagcactgtt tggactaggg gcccatctcg
      901 ggttaccgaa ttcagataaa ctccgaatgc cattcattca tatccgggag tcagactgtg
      961 agtgataaga tccatagtcg aaagggaaac agcccagacc accagctaag gtcccaaaat
     1021 atatgttaag tggaaaagga tgtggggttg cacagacaac tagg
//
LOCUS       DACDBG010000234         1063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDBG010000234 DACDBG010000000
VERSION     DACDBG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.243"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IVZ90_002996"
     CDS             complement(36..992)
                     /locus_tag="IVZ90_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263947.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          379 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca act
//
LOCUS       DACDBG010000235         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDBG010000235 DACDBG010000000
VERSION     DACDBG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.244"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IVZ90_002997"
     CDS             6..980
                     /locus_tag="IVZ90_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8263948.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACDBG010000236          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDBG010000236 DACDBG010000000
VERSION     DACDBG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.245"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IVZ90_002998"
     CDS             complement(26..913)
                     /locus_tag="IVZ90_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8263949.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DACDBG010000237          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDBG010000237 DACDBG010000000
VERSION     DACDBG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.246"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IVZ90_002999"
     rRNA            complement(<1..>994)
                     /locus_tag="IVZ90_002999"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDBG010000238          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDBG010000238 DACDBG010000000
VERSION     DACDBG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.247"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IVZ90_003000"
     CDS             <1..365
                     /locus_tag="IVZ90_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8263950.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..914
                     /locus_tag="IVZ90_003001"
     CDS             366..914
                     /locus_tag="IVZ90_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263951.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          384 a          137 c          204 g          242 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacagagta
      541 gaggaagaac aaaaaaagtt ggcgcaccaa tcagaaagtt tgataaggtt tattccacca
      601 agcatccttt aaaactgtcg actttgttaa atgcgacttc cagagtttta gtagaaaagt
      661 atgagactaa tatgacaaga gatgaattgc ttagaaaagc tttagatgag tatattaaac
      721 agaatcttac gcaagaagat aagcaagatt tgtttaatga cgtggtgaaa gaactagact
      781 tgttcagaga aaaatatcca attgttccag aattagatga tcgtggagaa ataataagaa
      841 ctaccagtca gataaaacaa gataccgaag actcactgcg caaagggtgg ggaatgaata
      901 ataaagttag ataataatca aagaagaact aagtaactac ctttaaacgg tagttactta
      961 gttcttc
//
LOCUS       DACDBG010000239          956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDBG010000239 DACDBG010000000
VERSION     DACDBG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.248"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(253..>956)
                     /locus_tag="IVZ90_003002"
     CDS             complement(253..>956)
                     /locus_tag="IVZ90_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8263952.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          319 a          160 c          157 g          320 t
ORIGIN      
        1 cttattgaaa gtgaacagaa aataattgat acaagaaatc aggatttgaa cacgctaata
       61 aaaatattgt tagtaaaact atacgaggta gtcttttcgg atgcagagat tgtttaaata
      121 tttgatagaa tagaaactta aaaagtttaa taaaactcaa ttatataagt agtagatgaa
      181 tatgatggtt atttagatta tggtagcgtc aagaattata tgtaaatgga aaatccattc
      241 tgttaattac agttaaatca tcgactctaa tgtatcctga atttctttaa atcccttgtg
      301 aattctacct aaaaattttt ggttatattg attaaattga gaaatgaaga atctctctag
      361 agattcttca ttagggaatt gttcttttct tcgagtatat cgttttagct gcttattaaa
      421 gccttcaatc aggttcgtcg aataaattgt tcgtctgatg gcgtgaggga aattataaaa
      481 ggttaatagg gcaggattca agagtaaatt gacgactctt ggatactgct ttttccattt
      541 cctagtcata aagtcgatgt gatccaatgc ctcttctttt gaattcgctt ggtaaaccaa
      601 tttgaaatcc tcacaaattt cttttcgacc tcgaacacga actttatgag cgatatttct
      661 agatacatgc acacaacatt gttgaaattg ggcattcgga tacacactat ggatactttc
      721 ttcaatacag cttaatccgt ctgtcactac taataaaact tcttctaacc cacgttttct
      781 aagatcttga agaacttctt tccaaatata agcagactca gttggggcaa tagtaaaccc
      841 aagaacttct ttggttccat ccaaacgaat accaatcgta atatacactg cttccttcga
      901 aacagtctgt cgcttcacag ggatatgcgt cgcatccata aatataacgg aataat
//
LOCUS       DACDBG010000240          911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDBG010000240 DACDBG010000000
VERSION     DACDBG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.249"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            211..795
                     /locus_tag="IVZ90_003003"
     CDS             211..795
                     /locus_tag="IVZ90_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8263953.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNEIDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          323 a          126 c          153 g          309 t
ORIGIN      
        1 gatttagatg agtctattaa ttcttgtagg attgcttttt gtatcagtta ttctgctctt
       61 tctcattagt ttagctctaa ggaatgagtt cagtaataaa cgttaacgat tgaaacttca
      121 agtcctgtgt aagataataa agttaattca tcttattagg aggaagtacc catagaaacc
      181 agagattata ttataactca ccaaaagtta atagaaagtg gactaagcca ttttgaaaaa
      241 gaaggatttc agcaaactaa gataaggcaa ctgtgcctga ccgcaggcgt aactacgggt
      301 gctttttata aacattttac ttctaaggaa gcgttatttg aagaaattat tcagccgcat
      361 attaatgcgc ttattaatat gtataatgaa gagtttacca aatacctctt aaacttagaa
      421 aatatagata tagagaaaag ttggagagag aacttcaatg aaatagatcc atttattaat
      481 tatatttttg agaataaacg tatattcgat ttgattttat tccaatcagc tggaaccaaa
      541 tatcaagata ttgttgaaaa aattacatca tttgtgacga aaaagacagt ctgttgtcta
      601 gataagttaa agactatcgg aatgatcaat caaaatttag tctttaatga agaagaaatc
      661 cacttctaca tttactcatt ctacgctaca ttctatgata tattgaagca tagctattca
      721 aaagaaaaga ctatgctatt aaccagaaga ttgtatcaat tttctgttcc tgggtggatt
      781 gaattactga gttaattaaa aaaagcttgc attgagaatt gttctcagtt atgctttttg
      841 ataagatcgc actgttatct gaataacggt gcatttttat cagcttaaga taacagtgcg
      901 atcttatttt t
//
LOCUS       DACDBG010000241          895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDBG010000241 DACDBG010000000
VERSION     DACDBG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.251"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            328..>895
                     /locus_tag="IVZ90_003004"
     CDS             328..>895
                     /locus_tag="IVZ90_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8263954.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIV"
BASE COUNT          326 a          114 c          157 g          298 t
ORIGIN      
        1 ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg ttttgatgct
       61 tatcaactta ttcaacccgc tatccacttc aagtataatt tttccatacg ccaataaagt
      121 agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt gaaagctact
      181 tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca gaaaaaaatc
      241 taaactataa aatttagggt tgactaaaat ataatttttg tataccatat gatcgtgata
      301 atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa tgtttgttat
      361 aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc tttaacaggt
      421 attattggtc caaatggtgc tggaaaatcc acattgctta aagcaatgtt aaatattatc
      481 cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact ggtgaagatt
      541 gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt aaaagaatgt
      601 gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg
      661 caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa tcatcaaata
      721 ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct tgctcaaaat
      781 gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag tgaacgaata
      841 attatggata cgttaaaaaa attgaaacat cagggaaaaa cagtgctaat tgtgc
//
LOCUS       DACDBG010000242          894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDBG010000242 DACDBG010000000
VERSION     DACDBG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.252"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IVZ90_003005"
     CDS             188..391
                     /locus_tag="IVZ90_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263955.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            593..>894
                     /locus_tag="IVZ90_003006"
     CDS             593..>894
                     /locus_tag="IVZ90_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8263956.1"
                     /translation="MIFLKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSN
                     RPGLEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMD"
BASE COUNT          336 a          119 c          159 g          280 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca ctttcttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaa
//
LOCUS       DACDBG010000243          884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDBG010000243 DACDBG010000000
VERSION     DACDBG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.254"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..735
                     /locus_tag="IVZ90_003007"
     CDS             <1..735
                     /locus_tag="IVZ90_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8263957.1"
                     /translation="PHEYEPLPEDVQKTSKADLIFYNGVNLETGGNAWFTKLVKNANK
                     EENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNIEKQLAEKDPDNKK
                     FYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFSKAYNVPSAYIWEI
                     NTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDSIA
                     EKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            750..>884
                     /locus_tag="IVZ90_003008"
     CDS             750..>884
                     /locus_tag="IVZ90_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8263958.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     M"
BASE COUNT          357 a          117 c          165 g          245 t
ORIGIN      
        1 ccccacgaat atgaaccttt gcctgaagat gttcaaaaaa cttcaaaagc agatttgatt
       61 ttttataacg gtgttaactt ggaaactgga ggaaatgctt ggtttacaaa attagttaaa
      121 aatgcgaaca aagaggaaaa caaagactat tttgcagcaa gtgatggcat agatgttatt
      181 tacttagagg gtcagagtga gaaagggaag gaagatcctc atgcttggtt aaatttagaa
      241 aacggtatta tttacgctaa aaatattgaa aaacagttag cggaaaaaga tcctgataat
      301 aaaaaattct ataaagaaaa tctagataag tatattgaaa aattggattc tctagacaaa
      361 gaagctaaat ctaaatttgc ttcaattccg aatgataaaa aaatgattgt tacaagtgaa
      421 ggatgcttta aatatttctc gaaagcgtat aatgtgcctt ctgcttacat ttgggaaatc
      481 aacactgaag aagaaggaac accagatcaa ataaaacact tagttgaaaa attacgcaca
      541 acaaaagttc cctccttatt cgtagaaagc agtgtggatg atagaccaat gaaaacagta
      601 tcaaaagata ccaatattcc tatctattca acgattttta ctgattcaat tgcagaaaaa
      661 ggacaagatg gtgatagtta ctatgcgatg atgaaatgga acctggataa aattgctgaa
      721 ggcctttcga aataatttgg agggtaggta tggtacaaat taaaacgact actatactaa
      781 tttatttact agcaattcag gaattatcaa aaagaaaaaa gaaattaaaa aataatgatt
      841 tagcaaaagt tctaagtgtt agtccagcat ctgtaagtga gatg
//
LOCUS       DACDBG010000244          829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDBG010000244 DACDBG010000000
VERSION     DACDBG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.256"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..321)
                     /locus_tag="IVZ90_003009"
     CDS             complement(<1..321)
                     /locus_tag="IVZ90_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8263959.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDI"
     gene            complement(393..551)
                     /locus_tag="IVZ90_003010"
     CDS             complement(393..551)
                     /locus_tag="IVZ90_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8263960.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT          241 a          140 c          121 g          327 t
ORIGIN      
        1 tatatctttt gtattactaa tggttatatc actaggaggc ctgtttgatc taattgtttg
       61 acctaattca tatctaggaa gccatttaga ttcgtaaaat tttttaaacg ttatttttcc
      121 ttttaattct aatgagtttg cattctcctc tctcaaaact ttttctattt tcttcttcat
      181 atcattttcg gctttaatcg ccaattgcct atttggaaat gttttttgat acttttctga
      241 tgcaattcct gttatttctt taaacctttt tggatatctc agattagttt tatatttccc
      301 atttgaaagc tttgtaggca aaatcctcac tccttaaaaa aagtaaggca gtatatttca
      361 ctaattttag tatatgcggc gagaagaaaa tttcaagtta ttttaaatgt attcataaag
      421 tctttaatat cttctacgtc aaagcgaact acaccaccaa ctcttattac tgggaaatta
      481 tatatacgaa tccacttatc tagagtatta ttatcgattc ctagaaatgc acaggtttgt
      541 tttttattca ggtagactct ggattctata aaatcatctc ctaaaatagt agttatgaaa
      601 tttttgaatt gctttgggta agcacatagg aatttcacct ctccccagtt tacggcgagc
      661 cttacggaag tatcattatt tgctgctatt gtcatggcat tttgttttcc cagaacaaaa
      721 agaaatctct aagtatttgt cgctaatcag gtcatggcgt acttgagttt ctagctcgaa
      781 tagcagggat cgttattcaa aaattgtatt taattgtcaa tgatcaaaa
//
LOCUS       DACDBG010000245          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDBG010000245 DACDBG010000000
VERSION     DACDBG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.257"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            254..>823
                     /locus_tag="IVZ90_003011"
     CDS             254..>823
                     /locus_tag="IVZ90_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8263961.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNALKKIAKEKDTMIFKNTICVLSTTEFY"
BASE COUNT          300 a           93 c          155 g          275 t
ORIGIN      
        1 attagatgaa ttcttggcgg atatcagtct atttataatg tttattgatg taattgaaga
       61 aacatcaaga aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa atgataaaca
      121 aaaattattg gcaaatgaga tttggagtag tttagaagtc gaagaaagta aacaagaact
      181 tttgttgaaa tggatacttt ttcttgtaaa gaagttctat gtgaaaatac cagaaaccca
      241 ttgtctgata gttatgcacg gtaaaataac agcttctgcg atcgcaaggg aaacaaataa
      301 gttgctgaat acatatgttt atgaggcttt cgatatgcca attgaaggtg agactagtga
      361 cttaattaac ctaatcaaca atttttgtaa aagtatagat acgacgaatg gactcattct
      421 gctagttgat atgggatcat tggaacaaat gtatgaaaaa attgaatcca atgtaatagg
      481 tgatttggta attttaaaca atgtttctac agctttagcg attgaatgtg ggattcaagt
      541 ttgccaaaaa aagccaatta gtcattttta ccagatggat tttgattcgt ttcaagttaa
      601 agttcagtat tataaaggat tatcacaaaa gaaaaatgta atagtatctt gtctctctgg
      661 tgaggggatt tctgaaaaag ttaaggatat tttaaaacgg tatctcgata atcaagtgga
      721 gattttgatt tttgatttta acgcattaaa gaaaatagca aaggaaaaag atactatgat
      781 ttttaagaat acaatttgtg ttctttcaac cactgaattt tac
//
LOCUS       DACDBG010000246          797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDBG010000246 DACDBG010000000
VERSION     DACDBG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.258"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..90)
                     /locus_tag="IVZ90_003012"
     CDS             complement(<1..90)
                     /locus_tag="IVZ90_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8263962.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFI"
     gene            complement(74..797)
                     /gene="aph(2'')-I"
                     /locus_tag="IVZ90_003013"
                     /pseudo
     CDS             complement(74..797)
                     /gene="aph(2'')-I"
                     /locus_tag="IVZ90_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          282 a          130 c           56 g          329 t
ORIGIN      
        1 tataaaccca aatgaactgg tatcattaag aaatcctttt aaattatcaa tatcaaaaac
       61 catattatca tcaataaaca atagtttcaa taggataata ttcttcaact atatcttgat
      121 attcttttgc tttctcaata tctatatttc catacattct taatatatct tctccaaaat
      181 ttgttcctat ttcttcttca ctatcttcaa gtaagtatat aaaatcacaa tattcatcta
      241 taattccaga atctccaaaa tcaattattc cagttaatct attattgcca tctaacaata
      301 gatgattaca actaaaatca ttatggcata aacacttttt accctcaaaa actgttgttg
      361 catttagtct ttccataaaa ctttctatat aatctttttc tatatcagtt aaatcattat
      421 aaatagtttc acgcaacaat atatactctt ctaatacatt ttgtttatta tcaatagtac
      481 attcactaat atctgtataa tctaaaccgt gcatttgtct taaaaaactg gcaatatctc
      541 gttttaacaa attttgttct tcttctgaca tagtagaata aatttctggt gttaaaaaag
      601 ttcctttaat ttctttataa cctagtatag ataattcatc actaatatac gaatattcaa
      661 tattaggaat ttttacatta gtttctaaat ttgtatttaa aaaattatat attgcttttt
      721 cttttgcata accttttttc ttattagtac taaattttgt tttaaaaatg tattcattat
      781 taactaaata tgccaca
//
LOCUS       DACDBG010000247          779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDBG010000247 DACDBG010000000
VERSION     DACDBG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.260"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..640
                     /gene="tnpB"
                     /locus_tag="IVZ90_003014"
     CDS             <1..640
                     /gene="tnpB"
                     /locus_tag="IVZ90_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8263963.1"
                     /translation="EEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKL
                     KREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIK
                     NHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSS
                     QICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          289 a          123 c          146 g          221 t
ORIGIN      
        1 agaagaaatt atcgaattac ctaaaactaa ttccacaatt ggtattgact taggtattat
       61 ggactttgcg attctttctg atggacaaaa aattgataat aataaattca cgtccaaaat
      121 ggaaaagaaa ctaaagcgtg aacaacgcaa attgtctagg cgtgctttat tggctaaaaa
      181 taaaggtacc aatctatttg aagctaaaaa ttatcaaaaa caaaaacgca aagtagctag
      241 attgcatgaa aaagtaatga atcaacgtac tgattttctg aataagttga gtacagaaat
      301 gatcaaaaat cacgatatta tctgtattga agacttaaac acaaaaggta tgttgcgaaa
      361 tcataaatta gctaaaagta tttctgatgt ttcttggtca aagtttgtga ctaaattaca
      421 atacaaggct gactggtatg gacgaaaaat catcaaagta gataaatggt ttccgtctag
      481 tcagatttgt tcagagtgcg gacataaaga tggcaagaaa tctcttgaaa ttcgagaatg
      541 gacttgtcct gtttgtcata ctcatcatga tcgtgatatc aatgctagta tcaatattct
      601 gaccgaaggt ctaagactac attctatggg attggcttag atccaatcat caagaaccgt
      661 aggaactacg gggatagctt ggtaaataag agacaccgct gttagtaaag aaatacgcta
      721 tcaagtatgc tctattccca agaagctccc acttcaagcg ttaagaaccg tcaggttta
//
LOCUS       DACDBG010000248          756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDBG010000248 DACDBG010000000
VERSION     DACDBG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.261"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..442)
                     /locus_tag="IVZ90_003015"
     CDS             complement(152..442)
                     /locus_tag="IVZ90_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8263964.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          261 a          123 c          111 g          261 t
ORIGIN      
        1 ttgcgagcag caactagaat aatttaaaca ttattaaact gtctatattg ttactaataa
       61 ttcccttctt atagtgcatt ttcttagaaa aaaacaatat tacgatatca tgatattaag
      121 atatcgtaat attatacaat tgtgattgat attatatatg tttttttatt tttttatcat
      181 gtttaatctt gaaggaatca tattcttttt ctaaagacag gagcttttca taaacatcag
      241 acatgtttaa actagaggcc aggcttaaat cataaacgaa tgaagcaggt aatgatttta
      301 ttctacgttg tcgggttatc cagcttgcaa ccgttccttg tgggtaggca tataactcac
      361 aaaattgttc tacagtgatt cctaaattct tgataatgta aacattgatc gggtgcgagt
      421 aaacaaatgt attgatagac atggtgcgta agcctacttt cttttattat tgcgtttgca
      481 ataatagttt aacacacgca ttattctagt atctactcct atttgtaaaa acaaaaaagg
      541 attcatagag ataactctat agaatccgta aaatacctta tatatataac gggaaaatat
      601 aaaacctaaa attatgataa cctgctgcag cttctacata gaaagagaag cgctcaacac
      661 caacctcatt tatcaaacgt tggccataac cccaaatctc ttctgatagc tgatcaaagg
      721 ttatatactt tagctcatat tgctggataa tgttca
//
LOCUS       DACDBG010000249          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDBG010000249 DACDBG010000000
VERSION     DACDBG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.262"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..623
                     /locus_tag="IVZ90_003016"
     CDS             6..623
                     /locus_tag="IVZ90_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263965.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          286 a           94 c          139 g          232 t
ORIGIN      
        1 gaaccttggg ttttcaagtg ctttttggtt cattaatctt aaccatagtg tttttaaagt
       61 ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg aatagttctt attattttta
      121 ctgtgactgc agcaacagaa gcaaattatt cgcataaaat tcagtcgtat aaaggtcgtt
      181 tagttatgac caaaaaagat gttacacgtt atacatcctc aagaactgga ggcctatatg
      241 actatgcacc attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag
      301 ttggtgatcg gtgggagaat gcatctcata aagcatataa ttccaaaata gtctatacag
      361 tagaagatgc cgatgagcaa aatatcacat tacctgtatt caattattgg ggaacagttg
      421 tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa tggtttagtg tctattaaag
      481 gtacggggaa aagaactata gttgaggttt catctacgta tccaactacg atgattctat
      541 ctttattaat tagttcaata acttttatat tcttatttac acgttattta ataagatgct
      601 gcggtgaaat gaaaatagat tagatagata ttataaatat gttgattcta ttttcatagc
      661 aatggagaaa atacattagc aatatataaa agaaggtatc aagagtcaca gtgaatttat
      721 tccgaaaagt gaaaagagca aatataaaat t
//
LOCUS       DACDBG010000250          723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDBG010000250 DACDBG010000000
VERSION     DACDBG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.264"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..355
                     /locus_tag="IVZ90_003017"
     CDS             86..355
                     /locus_tag="IVZ90_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8263966.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="IVZ90_003018"
     CDS             348..605
                     /locus_tag="IVZ90_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8263967.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          277 a           85 c          136 g          225 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 ttt
//
LOCUS       DACDBG010000251          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDBG010000251 DACDBG010000000
VERSION     DACDBG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.265"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            259..540
                     /locus_tag="IVZ90_003019"
                     /pseudo
     CDS             259..540
                     /locus_tag="IVZ90_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            614..>716
                     /locus_tag="IVZ90_003020"
     CDS             614..>716
                     /locus_tag="IVZ90_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8263968.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT          257 a           91 c          125 g          243 t
ORIGIN      
        1 aagtttttga tcctttattt tcaagtctga tttggcagct tttcggcagc tcatttattt
       61 atggtcagaa atagatgctg aaattttaat ctattagcaa atagtccaga acgtatgaag
      121 aggagtcgga tatcatttgt cattaacacc ataaatattt ataaaattcg tgttcaccta
      181 caattaaatt taatatataa tattcgttgt aagggcttat aattttttgt taatgaacaa
      241 gataggaaga aacatttaat ggacagtttt cagagattgt cactagtaaa tcaatttgag
      301 ttattggcta aatttgagga ccaaaacaga tcttattatg aaagaaaaat cgaaatattg
      361 agagaaggtt acgagtatca ttatgatgat gaaatttgga gtgatctttc tgagccattt
      421 ccgaaagaag attctcgatt tgtattagat gtattgaaca tgtatcgaga tatcaatttt
      481 agtaaatcaa agctaaactt agatgaaaac caaattacta attcatacta tactcatttc
      541 ggttctgttg caaagttttc caaaaaatct attttagtgt aaaattgaga aaaaagacag
      601 agaggacaga gtaatgaatc attttaaagg caaacaattc aaaaaagacg tcattattgt
      661 cgctgttggt tactacctgc gttacaatct aagctatcgt gaagttcagg aattgt
//
LOCUS       DACDBG010000252          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDBG010000252 DACDBG010000000
VERSION     DACDBG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.266"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            93..422
                     /locus_tag="IVZ90_003021"
     CDS             93..422
                     /locus_tag="IVZ90_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8263969.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IVZ90_003022"
     CDS             412..699
                     /locus_tag="IVZ90_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8263970.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          272 a           80 c          136 g          228 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttct
//
LOCUS       DACDBG010000253          711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDBG010000253 DACDBG010000000
VERSION     DACDBG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.267"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>711
                     /locus_tag="IVZ90_003023"
     CDS             <1..>711
                     /locus_tag="IVZ90_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8263971.1"
                     /translation="YLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVV
                     QGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFR
                     RSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDI
                     EKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENS
                     NGLLRRDGLLKSMDFNSV"
BASE COUNT          240 a          121 c          142 g          208 t
ORIGIN      
        1 tatatctgtt cttcaagcaa ggaaaatcag ccttagaata ttatcaacag tataagaaaa
       61 acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga ggaacaatca gaatatattc
      121 aaagaaaggt tgttcaagga tggacacccg atgtgattgt tggtcgtgca gcgtttccta
      181 ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa ggggctattt gattcttctg
      241 acttaccgat gaaaggcaag cgtaaaccga atggacatca agaaagacgt ggaaaacaaa
      301 ctttccgccg ctctattcat gaacgtgaaa aggattatag ccaattctca aatgagtttg
      361 gtcaccttga aggtgacact atcgtaggtc tgaaacataa aagtgctgta attaccttag
      421 ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa gcgattgata
      481 ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa aaacctattc aaatccatca
      541 cttttgattg tggaaaggaa ttttcaaatt ggaaacagat cagtaatgtc aatgatattg
      601 ccatttattt cgctgatcca ggaacgccgt ctcaaagagg cctaaacgag aattctaacg
      661 gattgttacg tagagatggt ttattgaaat ctatggattt caattcagta g
//
LOCUS       DACDBG010000254          708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDBG010000254 DACDBG010000000
VERSION     DACDBG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.268"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..>708
                     /locus_tag="IVZ90_003024"
     CDS             290..>708
                     /locus_tag="IVZ90_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAP8263972.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELE"
BASE COUNT          267 a           79 c          117 g          245 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagta
//
LOCUS       DACDBG010000255          701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDBG010000255 DACDBG010000000
VERSION     DACDBG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.269"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..436)
                     /locus_tag="IVZ90_003025"
     CDS             complement(<1..436)
                     /locus_tag="IVZ90_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8263973.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          214 a          119 c           95 g          273 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc atttttatta
      661 ataaaatggt atattttata ttgttataaa aagagcccaa c
//
LOCUS       DACDBG010000256          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDBG010000256 DACDBG010000000
VERSION     DACDBG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.270"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..667
                     /locus_tag="IVZ90_003026"
     CDS             <1..667
                     /locus_tag="IVZ90_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8263974.1"
                     /translation="GKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYR
                     WVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKK
                     RDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNL
                     IEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IPA"
BASE COUNT          239 a          122 c          139 g          197 t
ORIGIN      
        1 aggaaagcaa tttcagcagg atgtgattat tgtagccgtg ggctactatc ttcgttataa
       61 ccttagctat cgtgaagttc aagaaatctt atatgatcgt ggcattaacg tttctcatac
      121 gacgatttat cgttgggtgc aagaatatgg caaactactc tatcaaattt ggaaaaagaa
      181 aaataaaaaa tccttttatt catggaaaat ggatgaaacg tacatcaaaa ttaaaggaaa
      241 atggcattat ttgtatcgag ccatcgatgc agatggttta accttggata tttggttacg
      301 taaaaaacgg gacacacaag cagcctatgc ttttcttaag cggttagtga agcagtttga
      361 tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact
      421 aaaagaatac ggcttttatc aagggacaga acatcgtacc attaaatacc tgaataattt
      481 gattgaacaa gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
      541 tgcctctacc acgattaaag gcatggaagc cattcgagga ttatataaga aaacccgaaa
      601 agaaggcact ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc
      661 agcttaaatc atagataccg taagggattt tattctt
//
LOCUS       DACDBG010000257          691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDBG010000257 DACDBG010000000
VERSION     DACDBG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.271"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..632
                     /locus_tag="IVZ90_003027"
     CDS             <1..632
                     /locus_tag="IVZ90_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8263975.1"
                     /translation="VHISTSDPEELLNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKL
                     IRELDPLGKIIYVTSHTEMSMKILKSNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQ
                     MSQIQKDIFKIEFNDEIKFLPIKDIQYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEK
                     MHRNFIRCHKSYVINTQNVRSINKKTREVTLANGETIPASIRGLKKLIS"
BASE COUNT          247 a          132 c          118 g          194 t
ORIGIN      
        1 atgtccatat ttctacctct gatccggaag aacttctcaa tgcagccaag atcagaactt
       61 cttcttttgg tatttacttt ttagatatcg aactagaaga cagtgaaatg gatggtgtag
      121 cgatcggtaa attgatccgt gaattagatc ctttaggaaa aataatctat gtgacttccc
      181 atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga accgactgat tatatcgtca
      241 aagaagatct ttatgattta aaagaaaatg tcgagcatat cctgagcaaa attttcgaag
      301 attttcaaat gtcccaaatc caaaaagata tcttcaaaat agagtttaat gatgagatca
      361 aatttttacc gatcaaagac atccaatatt tttctacttc gctaggagcg ccacataaac
      421 tggaagtcca tttgactcat gcccaattac aattctatga gaagatcaaa gaaatcgaga
      481 aaatgcatcg gaattttatc cgttgtcata aatcctatgt tatcaataca caaaatgtgc
      541 ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa cggcgagacc atcccagcct
      601 ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc gaatagtttg gaccgactaa
      661 atgaccagcc gatccaaacc cttcggttct t
//
LOCUS       DACDBG010000258          690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDBG010000258 DACDBG010000000
VERSION     DACDBG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.272"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..554
                     /gene="spxA"
                     /locus_tag="IVZ90_003028"
     CDS             156..554
                     /gene="spxA"
                     /locus_tag="IVZ90_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8263976.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          241 a          103 c          133 g          213 t
ORIGIN      
        1 tatattaacc ttagataact ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt
       61 taaaagttga ttatgtataa tagaaacaga tgggagatta ttatgatctt ctgttacaaa
      121 aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt aacattatat acttccccaa
      181 gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg
      241 aacgtaatat tttttcagaa ccattgaaca gttctgaatt aaaagcgatt ttacaaatga
      301 cagaagacgg aacagaagaa atcatctcga cacgttccaa agtattccaa aaactgaaca
      361 tggatttaga tgatctgccg ttacaagaat tattggaact tgtgcaaaac aatcctggat
      421 tgcttcgccg tccaatcatg attgatgata aacgtctgca agttggtttt aatgaagatg
      481 aaattcgtcg gttcctaccg cgcgatgtac gacaattaga attacgtcaa gcacaattga
      541 tggctggatt atagaaggat aatacgaaac ttggaaaaga ctggctcttt gtcaaatgat
      601 gttggtaaag agaaatctta gaataatttg gacagaacag aaagaagaaa acctctttct
      661 gttctgttta tttggttaaa aatgttttgc
//
LOCUS       DACDBG010000259          684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDBG010000259 DACDBG010000000
VERSION     DACDBG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.273"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>684
                     /locus_tag="IVZ90_003029"
     CDS             <1..>684
                     /locus_tag="IVZ90_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8263977.1"
                     /translation="LFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVSAAIPIIGSYF
                     ILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSL
                     FLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVL
                     IFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDY
                     KNLLLSLKE"
BASE COUNT          230 a           72 c          101 g          281 t
ORIGIN      
        1 cttattttat atgactaata ctgatcttaa caaaagaaaa tatctttttg tatgtttatt
       61 gataccactc actttttttg ttgaattatt tactgatgtt tccgcagcta tacctataat
      121 cggaagttat tttatattaa aggaaaagaa gaaaactaat atagtgcttt taaataaatt
      181 actattatgt atgcttattg atgatttcag atctattatt acttccatgg ttatgacgta
      241 tatgttttca tttgaaggga taaaagggta tggttatgta gtattccaga taatgcttga
      301 ttctttgttt ttgctgatgt ttatttggtt atatagaaaa ttagctataa ataggattgt
      361 tgaacagtcc agttctaaac ttactgctat attcatgatt tatttactta caattggttt
      421 atttgtttct tatgttgctc atcaatatca agtatttgat cattttgtgt ttggtgtctt
      481 aatattttta atcattcaaa tgtttgttgt tctattttta tttattagaa taactattag
      541 gcaacgagaa caatatgaac agcaattgaa aagaaaagaa ttggtttatc taaaaaaata
      601 tactgattca ctggaaaaag atcaagaaaa atttgccaga tttagacatg actataaaaa
      661 tttattgctg agtttaaaag aaat
//
LOCUS       DACDBG010000260          644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDBG010000260 DACDBG010000000
VERSION     DACDBG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.274"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..>644
                     /locus_tag="IVZ90_003030"
     CDS             278..>644
                     /locus_tag="IVZ90_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8263978.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNY"
BASE COUNT          236 a           82 c          101 g          225 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatt
//
LOCUS       DACDBG010000261          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDBG010000261 DACDBG010000000
VERSION     DACDBG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.275"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IVZ90_003031"
     CDS             complement(<1..270)
                     /locus_tag="IVZ90_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263979.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="IVZ90_003032"
     CDS             complement(276..>635)
                     /locus_tag="IVZ90_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8263980.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DACDBG010000262          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDBG010000262 DACDBG010000000
VERSION     DACDBG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.276"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..609
                     /locus_tag="IVZ90_003033"
     CDS             <1..609
                     /locus_tag="IVZ90_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8263981.1"
                     /translation="QKNTKAFTNGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIP
                     EGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVL
                     TGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTEL
                     HRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQGS"
BASE COUNT          228 a           85 c          127 g          185 t
ORIGIN      
        1 caaaaaaaca cgaaagcttt taccaatgga aaaccattgt gttattatat actttcaaca
       61 cttttaaaag taaaacagat tgataaagtg tatgtctatt gtagtaaccc agatattaaa
      121 gaatttatac cagaaggtgt aacattttta aagagatcta gttccttaga tcaggataca
      181 acgagtatta cacaggtatt agccggtttc acaaaagaag tagaggctga tgtatatgta
      241 atgacacaca caacagcacc atttattagc gaaaaatcta ttgaaaaagg actagatgcc
      301 gtgctgacag gagaatatga ttctgcattt gctgcaaaaa aattgcaaga tttcttatgg
      361 aaagacggtg aaccatttaa ttatgaattg gacaatattc ctagaacaca ggatttgccg
      421 cctttatacg aagaaactag tggattttat atttataatc gcaatgtgat gaccgagttg
      481 catagaagaa ttgggaaaaa accttgcata gttgaagtag gtgagattga gtcagttgat
      541 attgatgaag ccgaggattt tatgattgct gatgcgattt ataatcatct tattttacaa
      601 ggtagttaag acagggggaa ggtta
//
LOCUS       DACDBG010000263          605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDBG010000263 DACDBG010000000
VERSION     DACDBG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.277"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..233
                     /locus_tag="IVZ90_003034"
     CDS             30..233
                     /locus_tag="IVZ90_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263982.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYEIEYWLTKLEN"
     gene            248..>605
                     /locus_tag="IVZ90_003035"
     CDS             248..>605
                     /locus_tag="IVZ90_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263983.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMAS"
BASE COUNT          235 a          100 c          121 g          149 t
ORIGIN      
        1 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
       61 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
      121 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
      181 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
      241 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
      301 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
      361 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
      421 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
      481 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
      541 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
      601 tagcg
//
LOCUS       DACDBG010000264          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDBG010000264 DACDBG010000000
VERSION     DACDBG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.278"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..>575
                     /locus_tag="IVZ90_003036"
     CDS             51..>575
                     /locus_tag="IVZ90_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263984.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYEL"
BASE COUNT          221 a           74 c          127 g          153 t
ORIGIN      
        1 tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata atgaataagg
       61 tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaaacaaa gaacacttga
      121 atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta
      181 ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac gagtcaaacg aaggttacag
      241 tcagccataa tcgtttagac gtgtataaac gtcgtcctga cacattaaaa aaaggaacag
      301 agcttctacg actagaaaca gaaaaatata tcatgaatgt ttacctagaa aggagtgagc
      361 tggtcgaaaa ctttgtcagc tacacgattt gtaggaaaca ctattactca gaatcagaat
      421 attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca
      481 aagatgcgac ggtagacgat tattaccttg tatttactag atttttgacg tgtgggacgg
      541 aatctatcgt gttaatggga atggattatg aactc
//
LOCUS       DACDBG010000265          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDBG010000265 DACDBG010000000
VERSION     DACDBG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.279"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..>575
                     /locus_tag="IVZ90_003037"
     CDS             51..>575
                     /locus_tag="IVZ90_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263985.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYEL"
BASE COUNT          222 a           77 c          129 g          147 t
ORIGIN      
        1 tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata atgaataagg
       61 tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaagcaaa gaacacttga
      121 atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta
      181 ttcaaagagt ggaagaacag ttgtatatgg aaagcgaaac aagtcaaacg aaggtcacag
      241 tcagccataa tcatttagac gtgtataaac gtcgtcctga aacggtaaaa aaaggaacag
      301 agcttctacg actagaaaca gaaaaataca tcatgaatgt gtacctagaa aggagtgagc
      361 tggtcgaaaa ctttgtcagc tacacgattt gtaggaaaca ctattactca gaatcagaat
      421 attattcaat tttcgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca
      481 aaaatgcgac ggtagacgat tattaccttg tatttactag gtttttgacg tgtgggacgg
      541 aatctatcgt gttaatggga atggattatg aactc
//
LOCUS       DACDBG010000266          574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDBG010000266 DACDBG010000000
VERSION     DACDBG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.280"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>574)
                     /locus_tag="IVZ90_003038"
     CDS             complement(<1..>574)
                     /locus_tag="IVZ90_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8263986.1"
                     /translation="FPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYL
                     FDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYS
                     MDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINR
                     SSVDFSISNALRTAYIATQHINNQDFTPSGS"
BASE COUNT          179 a          106 c           76 g          213 t
ORIGIN      
        1 gggaaccaga aggtgtaaaa tcttgattgt taatatgttg agttgcaata taagctgttc
       61 ttagagcatt agaaatagaa aaatcaacac tacttctatt aatggacgta aaaaaagttg
      121 tagcacaatc cccaatagca tatatatctt ttatatttgt ttgctggtat tcattagtta
      181 gatatgctcc actaacatct ctcaagagtt ccttttcccc aattggacta ttagatagaa
      241 atcctatagc ttgtacgacc atatccatag agtattgggt accacttgta gtgactattt
      301 tttcaatctt ttttcttcct aaatattttt taggactttc tgatagacaa agatgtattc
      361 cattttttga tagtatttct tcaactttat ctgaaaaact acggtcgtaa taagtcgaca
      421 acactctatc tgcaacatcg aaaagatata cttctttccc tctttttctt atggcttcag
      481 caatttcaac tccaatatat cctgctccaa taattcctac ttttttaata gtactatcat
      541 ccaaagcttt aaaaatctct tttgcatttg gaaa
//
LOCUS       DACDBG010000267          558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDBG010000267 DACDBG010000000
VERSION     DACDBG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.281"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          185 a           99 c          119 g          155 t
ORIGIN      
        1 gggaatatgt agaagaagta gataacccag gcgacgactg gtacacgact ttaactgatt
       61 ttttagacac ctacacttac gacgatacag aagaaatcca aaatgaactt gaccgtaaac
      121 gaatgccata tcaacgattt gaaaggaaaa aaggtaaata accatttata ccgaaaagtg
      181 aggatgtgtg aaataagaac aaaaaacctt gaaattaagg gaatggttct tatttcgcac
      241 caatcgctcc aaccgccccc gccgcttagg tggtgcgaaa taagcgggca aagcccgata
      301 acctaagaga aagaaggaga tccatatgaa taaaatcatt cctctattgt tcgtactgtt
      361 tgttggcgag atacttatgc gtcatgctgc agctgtacat ttaaacttcg tagtattctt
      421 aggaatactg gtattttgtt tgatcttttg gcgatttgta ttagcccttg tgagtctact
      481 attcaagatg attgcagcca tatttttcat agggttattt cgatgaaagg agagagtact
      541 aatgccaatt gcaatagg
//
LOCUS       DACDBG010000268          547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDBG010000268 DACDBG010000000
VERSION     DACDBG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.283"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>547
                     /locus_tag="IVZ90_003039"
     CDS             <1..>547
                     /locus_tag="IVZ90_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8263987.1"
                     /translation="IRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSIT
                     LGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSV
                     VQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKS
                     NHTISEVSLGLGFSDSSHFSRV"
BASE COUNT          199 a           68 c           95 g          185 t
ORIGIN      
        1 tattcgaaat ttagaaaaca tggtttttaa tctgagtaat ggaataattc caagtgttag
       61 tggagatacg attcggtctg aaaaaaatta ttctatcatt gtttttgaaa agttagcaca
      121 aacgtctata acattaggaa tggatataat agaagcgtat caaagtagag atgctttgat
      181 acaagaaaac gaattagctg tatcgttacc tgaggtctta aaagtgaggg attctgggat
      241 tgtttattat actaaggaaa tcggaaaaac taagatagag catctttctc ccttaatttc
      301 ttctgtcgtt caattcatcg gcttaaatat ctataaaaga attactgtaa aggaaatagc
      361 taattatttt tctgtaagtg aaactaaatt aagagaaagc tttaagaatg aaatgcatat
      421 tacgatttat aattatatct ctaagcgaaa aatttctacg gctaaaataa tgttgaaatc
      481 aaaccatact atcagtgaag tttctttagg tttaggattt tcagattctt cacacttttc
      541 gagagta
//
LOCUS       DACDBG010000269          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDBG010000269 DACDBG010000000
VERSION     DACDBG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.284"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            266..>541
                     /locus_tag="IVZ90_003040"
     CDS             266..>541
                     /locus_tag="IVZ90_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8263988.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRK"
BASE COUNT          152 a           76 c          126 g          187 t
ORIGIN      
        1 tagattattc aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga
       61 catttagaca attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag
      121 atacaggttg aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg
      181 taggtgttgc tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag
      241 tcttatgtat catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt
      301 taatgtgttt atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg
      361 ttttaagcag gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc
      421 gattgagttt cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga
      481 ttgtttgtgt gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaaa
      541 a
//
LOCUS       DACDBG010000270          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDBG010000270 DACDBG010000000
VERSION     DACDBG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.285"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          202 a           61 c           74 g          197 t
ORIGIN      
        1 aattcatttt taccgatagg ctaaatcgta taaaaactaa ccacttttaa tcattatttt
       61 attcttagtt ttatgtgaga taaattaacc attttaagtt tacaaatgta atcaatttct
      121 attactttat aagtatcgtt tacattcgtc aacgatagag cttgaaatat ttttatttga
      181 aaattttgta tttagatgat tatgaactaa tacagtgtcg gaggatactt atgtatttaa
      241 actaaactat tttagaaaaa ccagtaatcc tttgaatttt ctattggaaa atgaagtaaa
      301 atattataag aataaccttt aaagttgggc aactattatt caaacatcta aacatactcc
      361 taagagaaat agatatataa tagactaagg aagcgtttta aatattgtag aaaatagaga
      421 aaatttatca gatcttcggt ttaattttta cagcgttaat cgctcttgat atgaatgagc
      481 tctgataaag ccgaagtaaa ttggcgattt gaaaagttca attatttgag aata
//
LOCUS       DACDBG010000271          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDBG010000271 DACDBG010000000
VERSION     DACDBG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.286"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>534
                     /locus_tag="IVZ90_003041"
     CDS             <1..>534
                     /locus_tag="IVZ90_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8263989.1"
                     /translation="HRFYYLKELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFN
                     SSIEVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILM
                     KIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKK
                     MELYKMKDQLPFDLSGGE"
BASE COUNT          216 a           61 c           98 g          159 t
ORIGIN      
        1 catcgttttt actatttgaa agaattaatt gatcaagttc tatttgttaa aaatagaact
       61 gtaaaaacta caactattca gttgttaaaa actgaaaaca ataacgcatt aaggaaattt
      121 gacattttta atagttcgat tgaagttccc tatatcgaaa aggtaagtgc agatgtatta
      181 aaagttgaag aaattactta tgaaaatatt ctaaaagaca taaattttga tgtcaaagtt
      241 ggagatgtta ttggtttggt agggaaaaac ggtgtgggga aaacaacctt actaagaata
      301 ctcatgaaga ttattcgccc taccaaagga aagataatcg aaaataaaaa aaatttgtca
      361 tcgccgtttt tagtaatgca agaaatggat taccaatttt tcacagaatc agtgaggagc
      421 gagttagccc tggggaatga aatagttagt aaagatcagc aagaaaaaat tttaaaaaaa
      481 atggaacttt ataaaatgaa agatcaatta ccgtttgatt tatccggtgg agaa
//
LOCUS       DACDBG010000272          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDBG010000272 DACDBG010000000
VERSION     DACDBG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.287"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..>529
                     /locus_tag="IVZ90_003042"
     CDS             33..>529
                     /locus_tag="IVZ90_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8263990.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          194 a           98 c           87 g          150 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACDBG010000273          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDBG010000273 DACDBG010000000
VERSION     DACDBG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.288"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IVZ90_003043"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IVZ90_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8263991.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDBG010000274          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDBG010000274 DACDBG010000000
VERSION     DACDBG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.289"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            57..>517
                     /locus_tag="IVZ90_003044"
     CDS             57..>517
                     /locus_tag="IVZ90_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8263992.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DACDBG010000275          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDBG010000275 DACDBG010000000
VERSION     DACDBG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.290"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..>514)
                     /locus_tag="IVZ90_003045"
     CDS             complement(107..>514)
                     /locus_tag="IVZ90_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8263993.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DACDBG010000276          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDBG010000276 DACDBG010000000
VERSION     DACDBG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.293"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDBG010000277          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDBG010000277 DACDBG010000000
VERSION     DACDBG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.294"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IVZ90_003046"
     CDS             complement(62..259)
                     /locus_tag="IVZ90_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8263994.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACDBG010000278          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDBG010000278 DACDBG010000000
VERSION     DACDBG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.295"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            222..>479
                     /locus_tag="IVZ90_003047"
     CDS             222..>479
                     /locus_tag="IVZ90_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8263995.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEY"
BASE COUNT          187 a           65 c           82 g          145 t
ORIGIN      
        1 ttcgtgatta cttgaacaaa ttttctaaga atcatcttcg tattatttga tcaaaataaa
       61 agtattttgt tgttattaac cgattaatca aataaaaaat gattatcagt tcatcgacat
      121 acacataata cctcgataca aaaggggtgt caatatgttg tgtgaattta tttacaacac
      181 cccctttttg aggtatacta attttaggag gtgtggtcaa tatgataaag cgtccaccaa
      241 taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta
      301 aaaaattaac acaaccagca tttggtctta tgattaacaa tggtcaattg attgacaaaa
      361 aaactatcta cgaatgggaa aagggaacct atttaccaat accagagcga ttatcccgaa
      421 ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatat
//
LOCUS       DACDBG010000279          466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDBG010000279 DACDBG010000000
VERSION     DACDBG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.296"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          190 a           47 c           49 g          180 t
ORIGIN      
        1 cataaagtat gcgctttcat ttttttggat gagattaata tcaataaata acgatgtaaa
       61 aatcatttat tatgctcttt tataaggata ttaattgatc taatttgaaa aaaatcaaaa
      121 tttaaatgtt ttaatattac aaataaaatc atgttattgt aaaatggtat aaaaaagcta
      181 aatattctat ttttgttatt ttattagcag gaaaatttaa atagttttac aagaatccgt
      241 agaaatatat tggttttgcc ttcagattaa tactggatca tattaatctg aatataatat
      301 atgaactaat aaatagctta acctcgctat tttgtttttt ctgatcacta tctatacagc
      361 attagtaaat agaattttaa aaataaaaaa attaagacaa aaaagtatca acataaacat
      421 atacatatac acgtttatgt tgatttactt aatttgtcct ttaaaa
//
LOCUS       DACDBG010000280          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDBG010000280 DACDBG010000000
VERSION     DACDBG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.297"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>461)
                     /locus_tag="IVZ90_003048"
     CDS             complement(<1..>461)
                     /locus_tag="IVZ90_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8263996.1"
                     /translation="QFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIA
                     NILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRY
                     VVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKF"
BASE COUNT          160 a           92 c           60 g          149 t
ORIGIN      
        1 aaattttgca gtctcattat ctccatattc tttaattgca gaaatttgcc aggcctgtat
       61 aaatattccc tgcaaagtat taattatttg aggtatttta tatgccacag acaataagcc
      121 attcgccgag acacctacta taaaggaaac cacataccta tcagctgtac tattaatcca
      181 ccatccaaca acagttgcaa ttaatggaat cgagtaattt agcatttctc tctttagctg
      241 aggatcttta gcctcaaaat cgatatattc ccatattctc actaatactg acagataagt
      301 agctgaaata atctgtgata aaatatttgc aatgaaaaaa ccaattaatc cccatttcag
      361 aataagcaag aagaaaatat tggcaattat tgttacaatt gtgctgataa tacctgctat
      421 gcccatagct ttaacttttt ctaacccttt agcaaattgt a
//
LOCUS       DACDBG010000281          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDBG010000281 DACDBG010000000
VERSION     DACDBG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.298"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IVZ90_003049"
     CDS             <1..>452
                     /locus_tag="IVZ90_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8263997.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DACDBG010000282          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDBG010000282 DACDBG010000000
VERSION     DACDBG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.301"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..185)
                     /locus_tag="IVZ90_003050"
     CDS             complement(<1..185)
                     /locus_tag="IVZ90_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8263998.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDG"
     gene            complement(204..359)
                     /locus_tag="IVZ90_003051"
                     /pseudo
     CDS             complement(204..359)
                     /locus_tag="IVZ90_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT          144 a           79 c           47 g          167 t
ORIGIN      
        1 ccatcttttt ttgttattga tattgaagaa ttagaacttt catataaagc cttttctatt
       61 tcattgcttg atttcattat tgttaaacaa gaatacaagc aagaaagaac tatagttaat
      121 atgataaaaa ttattaaagt tctatttttc tttcttgtaa tatatgctat agcatttttt
      181 atcactttcc taactactcc tctctatttt cttactccat aactaattgc atccactgga
      241 caagcatttt tacatcgtcc acatctgata cactccagat gattgcaatt ctcaactgaa
      301 tctttattca tttggcaaac ttttttacat tttccacaat taatacactt atccttatcc
      361 tgaatcggct acactagact agacagaaaa aataaggtgt gtagaataat aaaaaacaca
      421 ctggaggatt tttcatg
//
LOCUS       DACDBG010000283          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDBG010000283 DACDBG010000000
VERSION     DACDBG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.303"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>432)
                     /locus_tag="IVZ90_003052"
     CDS             complement(<1..>432)
                     /locus_tag="IVZ90_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8263999.1"
                     /translation="PVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTI
                     WKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVL
                     VSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIF"
BASE COUNT          152 a           79 c           54 g          147 t
ORIGIN      
        1 aaaaattaaa acaataccaa gaaacgcata attcacttta cttctaaaaa atgttttgag
       61 taaactaaag atatttaaca catagaaagc atagatacat ataaaagcaa ttataccact
      121 tgatacaagt acttgaagaa atccattatg catgtttcca tgtgctcttt ttagttcatt
      181 cacatctgta gtatcaagat ctactttttt caaaaatttt gcatcgctat tcccatagaa
      241 atcggtctct ccataaccaa ataacaaagt tggtcttata actttaagcg cagctttcca
      301 aatcgtaaaa cgaccagctg aaatttcgga gttatcacta gaatgctgta atattacttc
      361 tttttctgga agtggagttt tatctgattc atttgactct ataacttcta cttttctatc
      421 aatcgataca gg
//
LOCUS       DACDBG010000284          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDBG010000284 DACDBG010000000
VERSION     DACDBG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.304"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..423
                     /locus_tag="IVZ90_003053"
                     /pseudo
     CDS             1..423
                     /locus_tag="IVZ90_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          171 a           75 c           81 g           96 t
ORIGIN      
        1 aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag aatatgaact
       61 aacggcttca gcatttgaca aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga
      121 aaaagataat cttacgcctg aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca
      181 attagaaatg gaaaatgata ttttaaagca agcagcgctg atattcggac gaagagacaa
      241 gtaatcgatg cgaataagca tctttaccct atatcagcga tgtgcagaat attaggtcta
      301 tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa
      361 gtagtcgctg aagaatttat ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa
      421 gcc
//
LOCUS       DACDBG010000285          418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDBG010000285 DACDBG010000000
VERSION     DACDBG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.305"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>418
                     /locus_tag="IVZ90_003054"
     CDS             <1..>418
                     /locus_tag="IVZ90_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8264000.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVE"
BASE COUNT          174 a           61 c           96 g           87 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaag
//
LOCUS       DACDBG010000286          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDBG010000286 DACDBG010000000
VERSION     DACDBG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.306"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IVZ90_003055"
     CDS             complement(<1..339)
                     /locus_tag="IVZ90_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8264001.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDBG010000287          398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDBG010000287 DACDBG010000000
VERSION     DACDBG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.denovo.310"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>398
                     /locus_tag="IVZ90_003056"
     CDS             <1..>398
                     /locus_tag="IVZ90_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8264002.1"
                     /translation="TTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSIDGI
                     RIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAFYI
                     VDSFGMMKGKDLTRLFYMIEHNLEKDIQIG"
BASE COUNT          151 a           41 c           77 g          129 t
ORIGIN      
        1 tacgacctta gaagaattag cacagataat tcctgaaaaa agagaaggaa aattatttgt
       61 agcaatgatg aactatggcg aatataatat agaggatctt ccagattatg atggttcatc
      121 tattgatgga attaggattg cgtttcataa aaaaaattgc aatgaagcgt tgaaattatg
      181 tcgtgagata aagaaaaaag gctacttagt ttttgttcaa gctatggtat ctctcaatta
      241 tacagatgaa gagtttttag cagtaataaa aaatgtgaat cagtttaaac catacgcttt
      301 ttatattgtt gatagttttg gaatgatgaa aggaaaagat ttaactcgtc ttttttacat
      361 gattgagcat aatctagaaa aagatataca gataggtt
//
LOCUS       DACDBG010000288         7070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.110, whole genome shotgun sequence.
ACCESSION   DACDBG010000288 DACDBG010000000
VERSION     DACDBG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.110"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1102
                     /locus_tag="IVZ90_003057"
     CDS             <1..1102
                     /locus_tag="IVZ90_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8264003.1"
                     /translation="VSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLT
                     DGQTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVM
                     IAQAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYP
                     SYKESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLN
                     NVIETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQA
                     PNSGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNE
                     KTGTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1589..2053
                     /locus_tag="IVZ90_003058"
     CDS             1589..2053
                     /locus_tag="IVZ90_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8264004.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2037..2666
                     /locus_tag="IVZ90_003059"
     CDS             2037..2666
                     /locus_tag="IVZ90_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8264005.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3020..4498
                     /gene="msr"
                     /locus_tag="IVZ90_003060"
     CDS             3020..4498
                     /gene="msr"
                     /locus_tag="IVZ90_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8264006.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4545..4991
                     /locus_tag="IVZ90_003061"
     CDS             4545..4991
                     /locus_tag="IVZ90_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8264007.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5190..5705)
                     /locus_tag="IVZ90_003062"
     CDS             complement(5190..5705)
                     /locus_tag="IVZ90_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8264008.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            5822..7024
                     /locus_tag="IVZ90_003063"
     CDS             5822..7024
                     /locus_tag="IVZ90_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8264009.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2220 a         1221 c         1476 g         2153 t
ORIGIN      
        1 tgtctcagaa aatacaattt caaccgaaga accaacaact gaaacaacag aacaaacaca
       61 aaccgaagac agtacatcag cagaagctca gaacaatcca gagaatgcag aagaaatcac
      121 gaaccaattg acagatgggc aaacattgac acatacccat caagcagatt tgtctgaatt
      181 cgaaaactat actggctatg ctttttatgc tcgctcatct gctaattctc aacaagcgtt
      241 cattgatagt attgcctcaa ctgcacaaag tttggcaagt gccaatgact tatatgcatc
      301 agtcatgatt gctcaagcca tcgttgaaag tggctggggg aacagtactt tggcttctgc
      361 ccctaattac aatctatttg ggattaaagg aagctataat ggccaatctg ttacgatgcc
      421 tacttcggaa tatgtgaatg ggcaatggat tacggtgaat gccgctttta gaaaatatcc
      481 ttcgtacaaa gaatcattgc aagataacgt aacggtattg aagacgactt cttttcagcc
      541 aggcgtttac tactattccg gtgcatggaa aagcaatacg aactcttaca aagatgctac
      601 ggcttggcta acaggtcgtt atgccactgc gcctaattat ggttctacat tgaacaatgt
      661 aatcgaaacc tataatctga cacagtatga tacagcacca acttcaactt ctccgatgta
      721 tcgtctgtat aaccgtcata caggagaaca tttatataca ttgaatgccg gagaaaaaaa
      781 ttatctgcca actgtaggct gggaatatga agggattgct tggcaagcac caaacagcgg
      841 acaacctgtc taccgtttat acaatcctta cagtggagac catcattaca caatggcaca
      901 atctgagatc aactctttaa caaaaatcgg ttggcgttat gaaggtctaa gtttttattc
      961 tggtggttcc aagccaattt atcgactatt taatccaaat gaaaaaactg gtacccatca
     1021 ctataccttg agtgctaagg aaagagattg gctaacaccg atgggctgga gatatgaagg
     1081 aataggattt tatggttatt aaaaagaggt agtgaaaaag ctgccctgca tcaagtagta
     1141 agaacagcca aacaaaaaca gattttcatg ttttttgttt ggttgaactt attaccgcag
     1201 atgcaagttt ttgaacaccg tttactcaaa aaaggttgtg acaacatctt gtcataactt
     1261 ttttattact gtttaagcat actgatccaa tttctaatat tcgacgggat tcttgcaaaa
     1321 ggcgatttcg gaaatttttg gcggtgtaaa attatctact gcagaagagc tatcttctat
     1381 agtttgataa gaatcaataa tcttggtttt agcgttgtgc tttctttatc attttttccg
     1441 ttcattctac aaaaaatttc ttttctctat acttttctat tgagagcgtt ttttttatta
     1501 cacagctgat tctttgactt ttttcttttt ttattctatt attgacaagt caatcgttga
     1561 cgagtcaatt attagaaagg aggaaattat gtctttagaa gagattagtc accttttata
     1621 tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt gaagaaaata tcggtataag
     1681 tcttactaga tacgagatca tgatgagact gaaagaagaa aatccattgc tacaaagcag
     1741 tttgcaagaa aagttgcaga ttgatcaagg agcagttact cgtcatctga aggtattgga
     1801 agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac aaccgggaag tgtatgtagc
     1861 gctaacagaa aaagcccaga agaaaatcaa gcattgtgaa atggaccata aagatacagg
     1921 ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa caatttctaa cacttttaga
     1981 taaattgaat acccaacttt gcaatataac aacagaaacg aaagaggtta ataataatgg
     2041 aacaaaaaaa tgatttttca acaattatga ctggtagaag atccgttcgc gtatatgatg
     2101 aaacatacaa aatcccacac gaagaaatgc tggaaatgat caaagaagcg acgactgcac
     2161 cttcttctgt caatatgaaa ccatggcgct ttgtggtcgc tgaaagcgag gaagcaaaag
     2221 aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa tgatacttct tctgcaatgg
     2281 tcatgatttt tggcgatatg caatgttacg aatatggcga agaaatctac aaccaagctt
     2341 acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg atcattcctt
     2401 attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat tctagcttag
     2461 cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat ccaattggtg
     2521 gattcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt tatgttccag
     2581 ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt cgtttagcac
     2641 cagaaaagat cactatgttc gaataaaagg ttttcagtta ctattcagta gctattgctt
     2701 tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga ctgcatcgat
     2761 gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca tgtataaaca
     2821 acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag gtgaaagcta
     2881 tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag atgtgatgca
     2941 gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt gttttacatg
     3001 aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc tacaagtcag
     3061 ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc aagatcgtat
     3121 agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa tagccggtga
     3181 actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc tgcctcaatt
     3241 aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt tccagttaag
     3301 actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga tcgagttgat
     3361 acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa cccatctaga
     3421 tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta cgctggtttt
     3481 tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg aagtaaaaga
     3541 cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa aggaattgga
     3601 gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac gattggaaaa
     3661 atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca gtaaaaagaa
     3721 aaagcaacaa atcagactgg atcggttgtc ttcctctaaa caaaaagaca gtgtacaaaa
     3781 agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag gagaaacaac
     3841 caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg aactccacag
     3901 ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa cattactggt
     3961 aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg aaaatggttc
     4021 aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc ttctctctcc
     4081 gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg aagaatccat
     4141 cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta gtttgctgaa
     4201 tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa gtgggggaga
     4261 agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt tgctgttaga
     4321 tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc taatgcaaat
     4381 atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc gtacggctga
     4441 tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa atttttaaat
     4501 gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct acaaataaaa
     4561 aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg atgagcgcgt
     4621 ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt ctagtgcctg
     4681 gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg aaaaaaattg
     4741 cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact atttcatgct
     4801 tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg gaacaaggaa
     4861 tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg attgccgctt
     4921 taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa gcaattcaaa
     4981 gtaatgaatg atggaacact gttatttgaa aaaaggcctt ggcatgcttt ttgtcatagt
     5041 ctcttgaatg cagttctaga aaaatagtga tccgaacgat aggttttggc ctgtcgttcg
     5101 gatcgctttt tcttttccaa ctgtattgct cttttattga agataatttg ctcttcaata
     5161 gatatttcta gaaaggttct gtttatccct tactcaaaaa actgctgcat ttgttctctt
     5221 acttgctgac accattggag ttcctgctga caaagcataa ttctgcgctg gatcacaaca
     5281 taacgtccaa attcttgttt cttgtctagc gagaggtcat tcaatttttt ttgatttttt
     5341 tgcatgattt tgttcagctg ttgttctcta gtgaatagca gtccagtagc tgttgtttta
     5401 tcgagcgtcg aaaaagcata gatttttgct agaaattcat ctttttgcgt aggagcggga
     5461 gtttcctctt tgatccattc ctctacaaga gcacgtcctt catcagtcaa gtggtagatc
     5521 tttttttgag agtgctcgtc tttgtccaaa acggtcacat aaccctgctg gtctaacttg
     5581 gcaagcgttg tatagatctg actgtgatgg gcttcccaaa aaattgtgat cagctgcttc
     5641 aattcatacc cagaacgtgg tttggataaa agcacgcata ataatatata ggctaaagta
     5701 ttcataaaaa tcctacctct cgtcttattt tctttattat atcacagtaa agtttccacg
     5761 ttttacatgt gcatattgac atgtaaaata aatcatgtat gatgctaata gagagaaagg
     5821 gatggaaata ttggagaatc aaatagatat acaagaacaa acaaatgaag aaacaaaggg
     5881 gaaaagttca gcgctagttg tagcagctac ctgtatgatc gcttttatgg gagtgggtct
     5941 agtagaccca atcttaaaaa cgattgctgc acagttaaac gctacgccag ctgaaacgac
     6001 cctgttattt actagttaca tgttagtcac tggtgtggtg atgctattta caggtttctt
     6061 atctagtcgg atcggtttga aaaaaacatt gatgacagga ttgtttatca tcgttgtatt
     6121 cgctggtctt ggcggattat ctgggaatat cggaatgctg attgggttac gtgccggctg
     6181 gggtgtcggg aatgctttat ttgtctctac tgctttagca acgatcgtca gtattttagt
     6241 tggtaacaca gaaaaagcaa tcatgatgta tgaagcagca cttggctgcg gaatggctgt
     6301 tggccctcta gtcggtgggg tcttgggcag tggttcatgg cgttatccat tttttggggt
     6361 agcatttttg atgttttgcg gttttatagc cctagcggta cttttacctg aaactgagaa
     6421 accaaagaga aaagtaggcc tttgggaagg tgtcaaagcg ttaagcaatc ataaacttcg
     6481 ttcagttgga ctaatcgcat tgttatataa tttcggattc tttactttac ttgcgtatgc
     6541 gccattttta ttgattggct attctgaatt agaagtcggt tttgtatttt ttggctgggg
     6601 tgtgcttctg gctattgctt cgattttcgt tgcaccggct cttgagcgga agtttacgac
     6661 ttatcggaca attttagcag catttgtctt attcattatt tgtttgctgt tattaggagc
     6721 tggaacttct atgccagtcc ttgtaccgat cagcattgtg ttagcaggat ttttccaagg
     6781 aattatcaat acactgttga cgacgattgc tatggaaata ccaggtctgc aacgaaacgt
     6841 tgcttcgtcc agctatagtt ttgtccgctt tttcggtgga gctttagcac catttattgc
     6901 aggtgtgatt ggtgaacata ttggtgaaag ctatgcgttt tattttgcag gtgccattgt
     6961 tcttatcagt gtatgttgta tcatttatca ccgtaattat tttgctacaa atgaagaaga
     7021 ataagaaatc agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa
//
LOCUS       DACDBG010000289         4849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.129, whole genome shotgun sequence.
ACCESSION   DACDBG010000289 DACDBG010000000
VERSION     DACDBG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.129"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(229..891)
                     /locus_tag="IVZ90_003064"
     CDS             complement(229..891)
                     /locus_tag="IVZ90_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8264010.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1255..1342
                     /locus_tag="IVZ90_003065"
                     /pseudo
     CDS             1255..1342
                     /locus_tag="IVZ90_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            1400..2131
                     /gene="erm(A)"
                     /locus_tag="IVZ90_003066"
     CDS             1400..2131
                     /gene="erm(A)"
                     /locus_tag="IVZ90_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8264011.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2257..3039)
                     /locus_tag="IVZ90_003067"
     CDS             complement(2257..3039)
                     /locus_tag="IVZ90_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8264012.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3193..3570)
                     /locus_tag="IVZ90_003068"
     CDS             complement(3193..3570)
                     /locus_tag="IVZ90_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8264013.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(3577..>4849)
                     /locus_tag="IVZ90_003069"
     CDS             complement(3577..>4849)
                     /locus_tag="IVZ90_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8264014.1"
                     /translation="INFKGFRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNF
                     LDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDV
                     PSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISEL
                     CTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYL
                     FPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQH
                     IVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQ
                     WFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELL
                     AIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
BASE COUNT         1538 a          914 c          693 g         1704 t
ORIGIN      
        1 ttatcaagag taaattacaa aagaatcatt taaatcaata ctttcacttt gaatacatgt
       61 atttgaagtg gaaggtactt atttcaaaaa tagtaaaacc tgtatcttaa attacttaat
      121 agtaacataa gatacaggct gattttttat tcattgttat ttatactaaa gcacccgata
      181 gctctgaaaa caatcacaaa ttcaactttt caaagccaca gctttaagtt attttgtcca
      241 gacaaccccc attgcccaac ccattttatg gaattggcat ccaggcaaca acttttcata
      301 taaatcgtaa taattttgtt cagataggta cttatctgaa gctaaatgct caagccatga
      361 tttagatgtg ttgtgattat aaattctaat cgcatttttt attccaaatt tgaaacaatg
      421 cggtaaaaac tcttggattg cccctaattt ataaacatag gtaggcggtg tctcgacttc
      481 agatacatta tcaagaataa ctattcttcc ttcctcattc aacagttcct tcatttgctg
      541 tattacgctg gctatatcat ccaaatgatg aaaggttgtt cggcttacaa taaaatcaaa
      601 cttctcatta aaattaagtt gttctgcatt catattcaga tagaccgtat ttgttagttg
      661 acgtttagat ttggcaagat cgagcatttg attagaaata tcaatcccta ccacttcatc
      721 ataataactt gctaatttct ccactaacaa acccgagcca catccgatat ctaatgctct
      781 gcctttcttt ggagacatat tagacacaaa gaatgaataa tcattcaaaa gctcattcac
      841 gaaatcgtaa tcttctgcaa ccttatcaaa ctgtgattct attgtattca aaaagatccc
      901 ccattcctac tttatcgaca ttctttcatt acttaccact ttagatgttt tttcgttggg
      961 gataaaactt ccctttagac aattttatcc aaagacaata caacagtgca actttattaa
     1021 agtcactgtc ctttatcgca gcctttactt tttagtaaag acagtggctt ctcttatcaa
     1081 gtttcaaaac atattatttt gaagaaaacg tccatctgaa gtgtcaagtg caaaattaca
     1141 tataaaggtt tattctaaaa tgaaaagatg atacaatcat attcagttac ataaggaggt
     1201 ttcaattatg tgcaccagta tcgcagtagt agaaattact ttatctcatt cataatgaaa
     1261 aaaatggaaa ggagataaaa gtatgggtac tttttctata tttgttatta ataaagttcg
     1321 ttatcaacca aatcaaaatt aattggttat aatgaacgct taatgtcagt tcattataac
     1381 cagtaaggag aaggttataa tgaaccagaa aaaccctaaa gacacgcaaa attttattac
     1441 ttctaaaaag catgtaaaag aaatattgaa tcacacgaat atcagtaaac aagacaacgt
     1501 aatagaaatc ggatcaggaa aaggacattt taccaaagag ctagtcaaaa tgagtcgatc
     1561 agttactgct atagaaattg atggaggctt atgtcaagtg actaaagaag cggtaaaccc
     1621 ctctgagaat ataaaagtga ttcaaacgga tattctaaaa ttttccttcc caaaacatat
     1681 aaactataag atatatggta atattcctta taacatcagt acggatattg tcaaaagaat
     1741 tacctttgaa agtcaggcta aatatagcta tcttatcgtt gagaagggat ttgcgaaaag
     1801 attgcaaaat ctgcaacgag ctttgggttt actattaatg gtggagatgg atataaaaat
     1861 gctcaaaaaa gtaccaccac tatattttca tcctaagcca agtgtagact ctgtattgat
     1921 tgttcttgaa cgacatcaac cattgatttc aaagaaggac tacaaaaagt atcgatcttt
     1981 tgtttataag tgggtaaacc gtgaatatcg tgttcttttc actaaaaacc aattccgaca
     2041 ggctttgaag catgcaaatg tcactaatat taataaacta tcgaaggaac aatttctttc
     2101 tattttcaat agttacaaat tgtttcacta aattaaagta ataaagcgtt ctctaatttc
     2161 acaagaggac gctttattct tcccaaaaat tgttcaatat ttatcaataa atcagtagtt
     2221 ttaaaagtaa gcacctgtta ttgcaataaa attagcctaa ttgagagaag tttctataga
     2281 atttttcata tacttaacga gtgctttcac ctttgaatat agtccttccc acttatcatc
     2341 acactctccc cgatagcctt ttctagctat atccagtaaa gttacatgct ctttaggtaa
     2401 aagaggtata gcccattctg cagcgacatc tttcgaggta atttcaccag tagtcactgt
     2461 ttgccacatt cgagctaggg ttaaaattac attacgctca tcacctttta tcccctcaat
     2521 tagttctggc aaagaatcct taattgctct tcgaatatct gtcaaaggta cggagacaag
     2581 tatacttgaa gaatcaggac caaatagaga aatactattc tttcttgctt gtgctaaaac
     2641 aatagccaaa tcaggatcat agcttggttc ctgaatttgt ccattctcaa attcacccct
     2701 gagccactca ccgtatataa attctctttt tggaggatat tgccaaggga caacttcact
     2761 cctatttata accgtaactt caagtggtct aacagaatcc gtatttccaa tctttcctga
     2821 tatagtcatt agtctttctg ttagtttttt tcgagttaat tgaggtaaac tatgattcac
     2881 gacgactaga acatctacat cgctgttaat gcgtaaacca ccatttactg ctgaaccaaa
     2941 tagatatact ccaactattg aacttccaaa taaatctttt acgattttta atgtttgaat
     3001 cgcttgattt ggtatttttc cgttaatcaa attgctcatg atttcacctc gtaactgatt
     3061 atgttcatat aaagtttata ttgatactca atttacttac cctagattgg acatatacct
     3121 aaatgactgt tcaataaagc tgaccgttag cgtttaagta catcctttca caatttgtct
     3181 acagattaat aattattctt tattatacag atctccatat aatttttgaa tttggttctg
     3241 taatttttta ttttcttttt ctaattccat tactcttctt tttaaggttt taataaggat
     3301 ttcctccgaa cgagaacttt tcttgggttt tgagactaca tttgctgtta tttgacgctc
     3361 acgaagggat tcgattcttt gcctaatatc gtgttcctta taaagccatg atttagaaac
     3421 attagcttcc tttgctattg aattaaaatt aataacttta ccttcaatcg aaaatttaga
     3481 aatcgctttg tctacttttt cccttgtctt ttttgatttc tgcttcgcca aacgtacaat
     3541 ttctgttgta tttctaactt gtttatccat tgataattac cccgtcaaac ttccaatgat
     3601 ttgttctaaa cgctctttaa cacggctatt agtctctact tgtctttgcc attgtttatc
     3661 cttagctatg gctaataact cttctgtacg ctctaactgt tcttcgtgct gtggtaagaa
     3721 ttgcttactg gtacagaagt gagtgcaatc taagcatgca ttcgcatgtg gacaaccacc
     3781 tgctactact ggcaatctac aataaccatt tggaagcact tgtgcattta tatttttctt
     3841 gaaccattga agctctacat catcgacttc attatcttca tctagatcaa gcacatctcc
     3901 attattggta accagttttt cctgaaattt agtaaattca ttttttagag tttcatcaaa
     3961 gatatgagcg tatctgcttg tcatttctgg gctttcatgc cccaaaaatt tctgcacaat
     4021 atgctggggc atcccgttgt taatcattct tgttcctact gtatggcgaa aggcatgggc
     4081 atggaatcta taaatctcac ctgatttatc cactatattt tgctcataag ctaatttatt
     4141 taactcacct ctaaatgttt cttgttttaa tggcgatcca tcttttcttg gaaagaggta
     4201 ttcactatct ggaaattcct ctgaaacttt atcttcccga actttaataa gtaaagctac
     4261 ctctttagat attggaacta tatgctcctt tttcattttc cattgataat actttaaaaa
     4321 gaaatctcca tctttgtcct ctaatagaca gccttttttc aaggtgcaca attcacttat
     4381 cctcattcca cattcttgaa caatcatagt catcgtagct atatattcgg gtaatttatc
     4441 aagatgactg ttcaattgct ctaggacgaa ttcatctata aagcgtggtt ttgctcttgg
     4501 tattttcgga tagtcctcag aataaattaa tattttggaa ggaacatcat cccattctag
     4561 cctaagaagg gtactaaata gtccttccaa tatagagatc ctcccagtta ttgtactagg
     4621 ttttattccc atcatgttta gttcacttaa atatgcttca atttccactc tcgttaattg
     4681 gtgtactctc tgaacttgtt taaatttcat gtccagaaaa ttaaagaact ctttaagtct
     4741 ttgggcaata tcacttacat aggaaaagct atccatgttc aatctcaact tacaatatct
     4801 ttttacaagt tgtttaaaat atgtattccg aaacccttta aagttaatt
//
LOCUS       DACDBG010000290         4547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.136, whole genome shotgun sequence.
ACCESSION   DACDBG010000290 DACDBG010000000
VERSION     DACDBG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.136"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..436
                     /locus_tag="IVZ90_003070"
     CDS             <1..436
                     /locus_tag="IVZ90_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8264015.1"
                     /translation="QEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILEL
                     LREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTS
                     FKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(586..1527)
                     /locus_tag="IVZ90_003071"
     CDS             complement(586..1527)
                     /locus_tag="IVZ90_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8264016.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1653..2774
                     /locus_tag="IVZ90_003072"
     CDS             1653..2774
                     /locus_tag="IVZ90_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8264017.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3032..4534
                     /locus_tag="IVZ90_003073"
     CDS             3032..4534
                     /locus_tag="IVZ90_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8264018.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
BASE COUNT         1489 a          820 c          949 g         1289 t
ORIGIN      
        1 gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa
       61 tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg
      121 gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat
      181 gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg
      241 tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca
      301 tacatcattt aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc
      361 aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc
      421 tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca
      481 caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa
      541 aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag
      601 gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc
      661 atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg
      721 gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata
      781 agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc
      841 gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc
      901 tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca
      961 agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc
     1021 ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag
     1081 ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca
     1141 catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg
     1201 acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga
     1261 acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc
     1321 gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc
     1381 gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc
     1441 gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc
     1501 tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
     1561 ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag
     1621 tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca attgattcat
     1681 ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc
     1741 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
     1801 tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc
     1861 agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat
     1921 ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg
     1981 cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa
     2041 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
     2101 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
     2161 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
     2221 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
     2281 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
     2341 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
     2401 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
     2461 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
     2521 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
     2581 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
     2641 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
     2701 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
     2761 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
     2821 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     2881 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
     2941 tgtatgggtc tttttgtgtg tatccaaccc ttgtatcctc tttagccagt tctgaaaata
     3001 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     3061 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     3121 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     3181 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     3241 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     3301 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     3361 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     3421 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     3481 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     3541 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     3601 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     3661 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     3721 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     3781 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     3841 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     3901 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     3961 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     4021 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     4081 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     4141 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     4201 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     4261 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     4321 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     4381 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     4441 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     4501 atctactgag attatttctc ttgagagaat ctaacccttg taatctc
//
LOCUS       DACDBG010000291         2844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.175, whole genome shotgun sequence.
ACCESSION   DACDBG010000291 DACDBG010000000
VERSION     DACDBG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.175"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..195
                     /locus_tag="IVZ90_003074"
     CDS             <1..195
                     /locus_tag="IVZ90_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8264019.1"
                     /translation="ILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVC
                     QPRRPNSRIDKVRYMFNKIT"
     gene            293..1666
                     /gene="tet(L)"
                     /locus_tag="IVZ90_003075"
     CDS             293..1666
                     /gene="tet(L)"
                     /locus_tag="IVZ90_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP8264020.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2230..>2844
                     /locus_tag="IVZ90_003076"
     CDS             2230..>2844
                     /locus_tag="IVZ90_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP8264021.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEK"
BASE COUNT          894 a          419 c          582 g          949 t
ORIGIN      
        1 attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaactttc
       61 tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt
      121 gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc
      181 aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga
      241 tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa ttatgaagtg
      301 taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg ttttatcttt
      361 ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg caaatgattt
      421 taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa ccttttccat
      481 tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt tactcctatt
      541 tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt ctttcttttc
      601 cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc cagcactcgt
      661 aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat ttggtcttat
      721 tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa tgatagccca
      781 ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca ctgttccgtt
      841 tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata tcaaaggaat
      901 tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat atagcatttc
      961 ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca ggaaagtaac
     1021 agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg gagttctttg
     1081 tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt atatgatgaa
     1141 agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc ctggaacaat
     1201 gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag gtcctttata
     1261 cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt cctttctttt
     1321 agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg ggctttcgtt
     1381 caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg aagctggtgc
     1441 tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta ttgcaattgt
     1501 aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg aagttgatca
     1561 gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg tcattagttg
     1621 gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg ttaagggatc
     1681 aactttggga gagagttcaa aattgatcct ttttttataa caggaattca aatctttttg
     1741 ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt gtatcttttt
     1801 tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa attttattct
     1861 tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt ggcaagggtt
     1921 taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc ttgctgattt
     1981 ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca ggtttttttg
     2041 tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt ggtcggcact
     2101 gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt tatactttac
     2161 ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct aaaaaggagc
     2221 gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc tggactaaaa
     2281 ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga tattgaccat
     2341 gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta caacgaacgt
     2401 gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa agatgctgtt
     2461 cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact ggatccagga
     2521 gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata tggcaagcaa
     2581 aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca tttaggtgtt
     2641 gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca agaactgtta
     2701 tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt gaagcgtggt
     2761 gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca aactttggaa
     2821 aaagagattg attttctaga aaaa
//
LOCUS       DACDBG010000292         2603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.181, whole genome shotgun sequence.
ACCESSION   DACDBG010000292 DACDBG010000000
VERSION     DACDBG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.181"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IVZ90_003077"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IVZ90_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8264022.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>2603
                     /locus_tag="IVZ90_003078"
     CDS             2039..>2603
                     /locus_tag="IVZ90_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8264023.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLL"
BASE COUNT          858 a          404 c          536 g          805 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taa
//
LOCUS       DACDBG010000293         2603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.182, whole genome shotgun sequence.
ACCESSION   DACDBG010000293 DACDBG010000000
VERSION     DACDBG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.182"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IVZ90_003079"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IVZ90_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8264024.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>2603
                     /locus_tag="IVZ90_003080"
     CDS             2039..>2603
                     /locus_tag="IVZ90_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8264025.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLL"
BASE COUNT          857 a          404 c          537 g          805 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taa
//
LOCUS       DACDBG010000294         1462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.210, whole genome shotgun sequence.
ACCESSION   DACDBG010000294 DACDBG010000000
VERSION     DACDBG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.210"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..504
                     /gene="thyA"
                     /locus_tag="IVZ90_003081"
     CDS             <1..504
                     /gene="thyA"
                     /locus_tag="IVZ90_003081"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8264026.1"
                     /translation="WRHWETKDGGFIDQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSM
                     ALPPCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLIAHETGLAVGEF
                     VHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPA
                     IKAPIAV"
     gene            524..1051
                     /locus_tag="IVZ90_003082"
     CDS             524..1051
                     /locus_tag="IVZ90_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8264027.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1114..>1462)
                     /locus_tag="IVZ90_003083"
     CDS             complement(1114..>1462)
                     /locus_tag="IVZ90_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8264028.1"
                     /translation="LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIY
                     IFMGWLCVVAAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVML
                     GAGFMYFSVLFFT"
BASE COUNT          485 a          284 c          308 g          385 t
ORIGIN      
        1 tggcgtcact gggaaacaaa agacggtggc tttatcgatc aaatcaaaca agtgatcgaa
       61 gcaatcaaaa agactcctga ttctagaaga ttgatcgttt ctgcatggaa tccggaagat
      121 gttcctagca tggctttgcc tccttgtcat acgatgtttc aattttatgt tcaagaggga
      181 cggctaagct gccaattgta tcaacgcagt ggggatgtgt ttctaggcgt tccgttcaac
      241 attgcaagtt atgcgttgct gactcaccta attgcacatg aaacaggttt agcggttggc
      301 gaatttgttc acacattagg agatgctcat ctttatatca atcatatcga acaaatgaaa
      361 gaacaattga gccgagaaat acgcgcattt ccgactttag ttttgaatca ggaaaagcag
      421 tcagtatttg attttgagat ggaagatatc caattagaag gatatgaccc acatccggcg
      481 atcaaagctc ctatcgctgt ttagaatcag aaaggatgag aaaatgttag ctgcaatttg
      541 ggcacaggat gaaaacggat tgattggaaa agaagaccgt cttccttggc gtttgccaaa
      601 tgatctgaaa ttcttcaaac aaatgacaga agcaaataca ctagtgatgg gacgaaaaac
      661 attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag acgattgttt tgacgagaga
      721 ttcttcctat aaagcagaag gcgtgcatgt catgcatagt gtggaagacg tgcttgctct
      781 ggaaaaagaa acagacggta tctttttcat cgctggaggg tcagctgttt atcaagaatt
      841 tctgccattt tgtacgatat tataccgtac agtcatccat catgtattcg atggagatgc
      901 ttattttcca cctgttgact ggtctgattg gtcactgatc aatactagtc aaggagaaat
      961 ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat cagcgtaatg agaatcctgt
     1021 aaaaagcggg acaggcgaat tgacgatata aaaaagctat ggatagacta gctgttattt
     1081 agagctgtcc catccatagc tttatttttt ttattaagta aagaataaaa cagagaagta
     1141 cataaaacct gcacctaaca taacaaacaa atgccaaaca acatgcatat agcggataga
     1201 attcaagcta taaaagaatg cgcctatcgt gtaagaaagc ccgcctgcta ccatcaaaat
     1261 aacaccagtc attccaagag agtgataaag aggaacagca gccacgacac agagccaccc
     1321 cataaaaata tagatgatcg tagatacttt tgatactctc tcccgtttat gcaacgtcaa
     1381 cgctttatat ataatgccaa gaatcgcaaa caaccagatt aatacaaata aagtccaacc
     1441 tagccatcct cggatactca gc
//
LOCUS       DACDBG010000295         1208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.229, whole genome shotgun sequence.
ACCESSION   DACDBG010000295 DACDBG010000000
VERSION     DACDBG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.229"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IVZ90_003084"
                     /pseudo
     CDS             46..297
                     /locus_tag="IVZ90_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IVZ90_003085"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IVZ90_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8264029.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          385 a          205 c          297 g          321 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaa
//
LOCUS       DACDBG010000296         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.231, whole genome shotgun sequence.
ACCESSION   DACDBG010000296 DACDBG010000000
VERSION     DACDBG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.231"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="IVZ90_003086"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="IVZ90_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8264030.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DACDBG010000297          908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.250, whole genome shotgun sequence.
ACCESSION   DACDBG010000297 DACDBG010000000
VERSION     DACDBG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.250"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..743
                     /gene="erm(B)"
                     /locus_tag="IVZ90_003087"
     CDS             6..743
                     /gene="erm(B)"
                     /locus_tag="IVZ90_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8264031.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          347 a          155 c          137 g          269 t
ORIGIN      
        1 ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa aaagtactca
       61 accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag
      121 gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat
      181 tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact cgtgtcactt
      241 taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg
      301 ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccgtg
      361 cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc
      421 gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag
      481 cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata
      541 ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca
      601 atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca
      661 aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc
      721 tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg aaagttacac
      781 gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata actaccaatt
      841 aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta tttcttatcg
      901 atacaaat
//
LOCUS       DACDBG010000298          859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.255, whole genome shotgun sequence.
ACCESSION   DACDBG010000298 DACDBG010000000
VERSION     DACDBG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.255"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..755
                     /gene="aph(2'')-I"
                     /locus_tag="IVZ90_003088"
                     /pseudo
     CDS             1..755
                     /gene="aph(2'')-I"
                     /locus_tag="IVZ90_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            739..>859
                     /locus_tag="IVZ90_003089"
     CDS             739..>859
                     /locus_tag="IVZ90_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8264032.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGF"
BASE COUNT          355 a           58 c          144 g          302 t
ORIGIN      
        1 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
       61 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      121 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      181 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      241 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      301 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      361 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      421 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      481 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
      541 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
      601 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
      661 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
      721 ttgaagaata ttatcctatt gaaactattg tttattgatg ataatatggt ttttgatatt
      781 gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat
      841 aataaaatta taggatttg
//
LOCUS       DACDBG010000299          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.259, whole genome shotgun sequence.
ACCESSION   DACDBG010000299 DACDBG010000000
VERSION     DACDBG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.259"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            203..700
                     /gene="dfrG"
                     /locus_tag="IVZ90_003090"
     CDS             203..700
                     /gene="dfrG"
                     /locus_tag="IVZ90_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8264033.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          307 a           80 c          165 g          239 t
ORIGIN      
        1 acataccaaa ttacatacaa aatgatttat aaaatgcaat tgaaaatgat aattgaatga
       61 aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac
      121 taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt
      181 agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag
      241 agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa
      301 aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag
      361 agccttacct gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg
      421 ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat
      481 ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta
      541 catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga
      601 ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta
      661 ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa
      721 gaacagtaaa gataaagcac aagagcttgg gattacggct ctttgtcaat aaggactgat
      781 gggttgtaca a
//
LOCUS       DACDBG010000300          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.263, whole genome shotgun sequence.
ACCESSION   DACDBG010000300 DACDBG010000000
VERSION     DACDBG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.263"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            180..728
                     /gene="aac(6')"
                     /locus_tag="IVZ90_003091"
     CDS             180..728
                     /gene="aac(6')"
                     /locus_tag="IVZ90_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8264034.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          258 a          111 c          161 g          205 t
ORIGIN      
        1 atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta
       61 taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg
      121 agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta
      181 tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac
      241 tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga
      301 tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg
      361 gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct
      421 cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt
      481 ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa
      541 gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg
      601 aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg
      661 caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt
      721 ctcaataata aaaag
//
LOCUS       DACDBG010000301          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24015_2016
            SAMN12640372-rid9893113.guided.291, whole genome shotgun sequence.
ACCESSION   DACDBG010000301 DACDBG010000000
VERSION     DACDBG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640372
            Sequence Read Archive: SRR10041172
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743116.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640372-rid9893113.guided.291"
                     /isolate="E24015_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IVZ90_003092"
     CDS             <1..75
                     /locus_tag="IVZ90_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8264035.1"
                     /translation="EKLGGKNDYKDEIEYVYDYEKGDK"
     gene            76..>514
                     /locus_tag="IVZ90_003093"
     CDS             76..>514
                     /locus_tag="IVZ90_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8264036.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR"
BASE COUNT          222 a           33 c           94 g          165 t
ORIGIN      
        1 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag aatatgtata tgattatgaa
       61 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      121 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      181 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      241 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      301 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      361 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      421 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      481 gaccctcata aaaataatcc aagagcaata aggg
//
